pycom01g20050

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0013557
Physical Location & Seq
Forward (+)
18511008 .. 18513959
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g20050.3

Sequence Viewer

Length: 2760 bp
ATGAAAAAACCAACCATCTTTAATACGCGCACATGTTGCCACAGCAGATGTCCCTCTCCCTCCTTTCTCACTGATATTTCTACCCTCCAAAGTACCATTTTCCATAAACCCTCACTCGCTCGCTCGCTTACTCCCCCACTTCACTATCTCTTCCTCAACACTTCTTCTTCTTCTTCTTCCTCCTCATTGCCTGATGAAAATGGTAGAAAAACCAGAGAAATGGCAGGTTTTAACCCTAACAATCTTCATGTTCTTCAACATTGCCCTCTCTGCAAAAACCCCATTGACAAAAAAGACCTACATTGTCCAGATGGACAAGTCAGCCAAGCCAGAGTCTTTCTCCAACCACATAGATTGGTACTCATCAAAAGTGCAAGCCATCAACAAAGGGTACTCTACACTTACCAGAACGCCTTCCACGGCGTCGCCGCCCGGCTAAGCGAGCAAGAAGCCGAGCGGCTAGAGGAGCAAGACGGAGTCTTAGCGATTTTCCCAGAGACCAAGTACGAGCTCCACACCACAAGGAGTCCCCTGTTTCTTGGGCTCGAACCGCATGTCAGAGCCAGCACCACCAATGTCTGGTCACAACGAGTCACCGACCACGACGTCATTGTCGGGGTGCTGGACACGGGTGTCTGGCCGGAGAGCCAGAGCTTCAACGACACTGGCATGACCCCGGTGCCCGCCCACTGGAAGGGAGCGTGCGAAACCGGCCGCGGCTTCACCAAAAGCAACTGCAACAAAAAAATTGTTGGAGCGAGAATTTTTTACCACGGATATGAGGCAGCCACGGGGAAGATCAACGAGCAGACGGAGTTTAAGTCGCCGAGAGATCAGGACGGGCATGGGACCCACACTGCCGCCACAGTTGCCGGGTCTCCGGTGCGCGGTGCTAATCTTCTCGGGTATGCTCGTGGAACGGCCAGAGGAATGGCGCCGAGTGCGAGAATTGCGGCGTACAAGGTTTGCTGGGTCGGCGGCTGCTTCAGCTCGGATATTCTGTCGGCGGTGGACAGAGCGGTGGCGGATGGAGTCAATGTGTTGTCTATATCTTTAGGAGGAGGTGTGTCTTCTTATTACCGGGACAGTTTGTCCGTGGCGGCTTTTGGGGCGATGGAGATGGGAATTTTCGTGTCCTGCTCCGCCGGAAATGGAGGGCCGGATCCTGTTAGTCTGACCAATGTGTCCCCCTGGATAACCACGGTTGGAGCTAGCACCATGGATAGGGATTTTCCAGCTACCGTGAAGCTCGGAAATGGCAGGACTATTACCGGGGTTTCACTCTACAGGGGAAGGATGAAGCTGTCAACAAACAAGCAATACCCTGTTATGTATTTGGGAAGCAATTCTACTAGTCCTAATCCGAGCTCGCTGTGTTTGGAGGGGACGTTGGATCGCCGAGTTGTGGCAGGAAAGATTGTGATTTGCGACCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGAAGTGGTGAAAGAAGCCGGAGGAGTTGGGATGATCTTGGCAAACACAGCTGCAAACGGAGAGGAGCTTGTGGCGGATTGCCACCTTGTTCCGGCGGTTGCTGTAGGAGAAAACGAAGCAAGGGGAATCAAGCATTATGCTTCAACAAGCCCGAGAGCCACCGCAACTCTAACGTTTCTTGGAACTAGAGTTGGGGTTAGACCGTCGCCTGTGGTGGCAGCGTTTTCATCCAGAGGGCCTAATCTGGTAAGCCTTGAGATTCTCAAGCCGGATATGGTGGCACCAGGAGTGAACATCCTCGCCGCTTGGACAGGCGCATTAGGCCCGTCCAGCTTGCCAGCGGATCGTAGAAGGGTGAAGTTCAATATACTGTCCGGGACTTCAATGTCCTGCCCCCATGTGAGCGGCATTGCCGCTCTGCTCAAGGCAAGGCACCCGGATTGGAGTCCGGCTGCAATAAAGTCCGCCTTGATGACAACCGCTTATGTCCACGACAACACGCGAAAGCCTCTACAAGACTCCTCGGCAGCTTCAATTTCAACCCCGTATGATCACGGTGCAGGGCACATCAACCCGGGGAGGGCTCTTGACCCGGGATTGATTTACGACATTGAGGCGCAGGACTATCTTGAATTTCTCTGCACACAAAGGCTAACACCAACACAGCTCAAAGTTTTTACCAAGTACTCAAACAGAAGTTGCAAGAACAATCTAGCCAGTCCCGGGGACTTGAACTATCCGGCACTATCGGTTGTGTTCCCGGACAGAACAAATGTTTCTGTTTTGACACTCCACAGGACTGTCACTAATGTTGGCCCTGCGGTTTCGAATTACCACGCCATTGTTTCACCGTTCAAAGGTGCTAATGTGAAAGTCGAGCCTCGGACGCTGAAATTCACCAAAGCCAATCAGAAGCTGTCATACAAAATCATCTTCACTACGAAATCCCGGCAGGCGGTCCCGGAGTTCGGAGGGTTGGTGTGGAAGGATGGAGTGCACAGGGTGAGAAGCCCCATTGTTGTAGTGTGGCTAAACAAATTTTCTGTAATAGTTAGCATCCTTCCTCTGCTTCTTACTGTGATCTTCTATGAAAACGTAGATTACGCAAGTTCAATTCACATTGATTTTCTCAATTTCCATATTGATTCACATGTTTCCGCACTATTTTCATGTCACCACTCACAAGCACTTAGTAGGAGTTTTAGTAGTTTTTGCGAGGATGACAATTTCAGACATGCACGAAACAAAACAAATTTAAACATGACATGTTGTAGGAATCAAGATCCACGATATTTGTAG

Protein Analysis

920

Amino Acids

99.76

Weight (kDa)

9.27

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 5 cut(s) 605, 611, 632, 1183, 1846
AatII GACGTC 1 cut(s) 609
Acc36I ACCTGC 1 cut(s) 215
AccB1I GGYRCC 4 cut(s) 679, 934, 1741, 1893
AccB7I CCANNNNNTGG 1 cut(s) 579
AccBSI CCGCTC 4 cut(s) 457, 1019, 1866, 1877
AccII CGCG 4 cut(s) 28, 717, 888, 1963
AclI AACGTT 1 cut(s) 1634
AclWI GGATC 5 cut(s) 1157, 1170, 1401, 1812, 2738
AcoI YGGCCR 3 cut(s) 638, 712, 921
AcsI RAATTY 6 cut(s) 762, 1125, 2093, 2354, 2498, 2713
AcuI CTGAAG 1 cut(s) 970
AcyI GRCGYC 3 cut(s) 423, 606, 935
AdeI CACNNNGTG 1 cut(s) 2464
AfaI GTAC 6 cut(s) 94, 360, 393, 506, 959, 2147
AflIII ACRYGT 3 cut(s) 32, 2611, 2726
AhdI GACNNNNNGTC 1 cut(s) 1090
AhlI ACTAGT 1 cut(s) 1352
AjnI CCWGG 2 cut(s) 1190, 1744
Alw21I GWGCWC 3 cut(s) 513, 1370, 2460
Alw26I GTCTC 2 cut(s) 491, 882
Alw44I GTGCAC 1 cut(s) 2456
AlwI GGATC 5 cut(s) 1157, 1170, 1401, 1812, 2738
AlwNI CAGNNNCTG 1 cut(s) 2377
Ama87I CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
AoxI GGCC 7 cut(s) 638, 712, 921, 1157, 1697, 1783, 2275
ApaLI GTGCAC 1 cut(s) 2456
ApeKI GCWGC 6 cut(s) 785, 981, 1511, 1679, 1913, 1988
ApoI RAATTY 6 cut(s) 762, 1125, 2093, 2354, 2498, 2713
ArsI GACNNNNNNTTYG 2 cut(s) 540, 572
Asp700I GAANNNNTTC 2 cut(s) 413, 1345
AspLEI GCGC 5 cut(s) 30, 888, 937, 1778, 2080
AspS9I GGNCC 6 cut(s) 849, 1157, 1697, 1784, 2276, 2419
AsuHPI GGTGA 8 cut(s) 586, 715, 1480, 1828, 2301, 2350, 2476, 2627
AsuII TTCGAA 1 cut(s) 2288
AsuNHI GCTAGC 1 cut(s) 1211
AvaI CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
AvaII GGWCC 2 cut(s) 849, 2419
BaeGI GKGCMC 3 cut(s) 684, 2028, 2460
BamHI GGATCC 1 cut(s) 1162
BanI GGYRCC 4 cut(s) 679, 934, 1741, 1893
BanII GRGCYC 4 cut(s) 513, 546, 1370, 2047
BarI GAAGNNNNNNTAC 4 cut(s) 1333, 1365, 2366, 2398
BauI CACGAG 1 cut(s) 912
BbsI GAAGAC 1 cut(s) 1062
Bbv12I GWGCWC 3 cut(s) 513, 1370, 2460
BbvI GCAGC 6 cut(s) 797, 968, 1498, 1691, 1900, 2000
BccI CCATC 7 cut(s) 23, 305, 387, 1022, 1108, 1114, 2444
BceAI ACGGC 2 cut(s) 436, 936
BciT130I CCWGG 2 cut(s) 1192, 1746
BclI TGATCA 1 cut(s) 2011
BcoDI GTCTC 2 cut(s) 491, 882
BcuI ACTAGT 1 cut(s) 1352
BfaI CTAG 6 cut(s) 461, 1212, 1353, 1446, 1647, 2174
BfmI CTRYAG 2 cut(s) 1285, 1563
BfoI RGCGCY 1 cut(s) 938
BfuAI ACCTGC 1 cut(s) 215
BlpI GCTNAGC 1 cut(s) 437
BmcAI AGTACT 1 cut(s) 2147
Bme18I GGWCC 2 cut(s) 849, 2419
BmeRI GACNNNNNGTC 1 cut(s) 1090
BmeT110I CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
BmgT120I GGNCC 6 cut(s) 849, 1157, 1697, 1784, 2276, 2419
BmiI GGNNCC 8 cut(s) 681, 850, 851, 936, 1164, 1743, 1895, 2421
BmsI GCATC 1 cut(s) 2526
BmtI GCTAGC 1 cut(s) 1215
BpiI GAAGAC 1 cut(s) 1062
BplI GAGNNNNNCTC 2 cut(s) 324, 356
Bpu1102I GCTNAGC 1 cut(s) 437
Bpu14I TTCGAA 1 cut(s) 2288
BpuEI CTTGAG 3 cut(s) 1709, 1736, 1868
BsaHI GRCGYC 3 cut(s) 423, 606, 935
BsaI GGTCTC 2 cut(s) 491, 882
BsaWI WCCGGW 1 cut(s) 880
Bse118I RCCGGY 1 cut(s) 710
Bse1I ACTGG 3 cut(s) 670, 695, 2178
Bse3DI GCAATG 3 cut(s) 185, 259, 1869
BseBI CCWGG 2 cut(s) 1192, 1746
BseGI GGATG 8 cut(s) 1033, 1302, 1497, 1688, 1755, 2455, 2517, 2686
BseMI GCAATG 3 cut(s) 185, 259, 1869
BseNI ACTGG 3 cut(s) 670, 695, 2178
BseRI GAGGAG 6 cut(s) 172, 479, 1074, 1497, 1538, 1972
BseSI GKGCMC 3 cut(s) 684, 2028, 2460
BseX3I CGGCCG 1 cut(s) 712
BseXI GCAGC 6 cut(s) 797, 968, 1498, 1691, 1900, 2000
BseYI CCCAGC 1 cut(s) 969
BsgI GTGCAG 3 cut(s) 1472, 2040, 2086
Bsh1236I CGCG 4 cut(s) 28, 717, 888, 1963
Bsh1285I CGRYCG 2 cut(s) 715, 1432
BshFI GGCC 7 cut(s) 640, 714, 923, 1159, 1699, 1785, 2277
BshNI GGYRCC 4 cut(s) 679, 934, 1741, 1893
BsiEI CGRYCG 2 cut(s) 715, 1432
BsiHKAI GWGCWC 3 cut(s) 513, 1370, 2460
BsiHKCI CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
BsmAI GTCTC 2 cut(s) 491, 882
BsnI GGCC 7 cut(s) 640, 714, 923, 1159, 1699, 1785, 2277
Bso31I GGTCTC 2 cut(s) 491, 882
BsoBI CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
Bsp119I TTCGAA 1 cut(s) 2288
Bsp1286I GDGCHC 7 cut(s) 513, 546, 684, 1370, 2028, 2047, 2460
Bsp143I GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
Bsp1720I GCTNAGC 1 cut(s) 437
Bsp19I CCATGG 1 cut(s) 1218
BspANI GGCC 7 cut(s) 640, 714, 923, 1159, 1699, 1785, 2277
BspFNI CGCG 4 cut(s) 28, 717, 888, 1963
BspLI GGNNCC 8 cut(s) 681, 850, 851, 936, 1164, 1743, 1895, 2421
BspMI ACCTGC 1 cut(s) 215
BspOI GCTAGC 1 cut(s) 1215
BspPI GGATC 5 cut(s) 1157, 1170, 1401, 1812, 2738
BspT104I TTCGAA 1 cut(s) 2288
BspT107I GGYRCC 4 cut(s) 679, 934, 1741, 1893
BspTNI GGTCTC 2 cut(s) 491, 882
BsrBI CCGCTC 4 cut(s) 457, 1019, 1866, 1877
BsrDI GCAATG 3 cut(s) 185, 259, 1869
BsrFI RCCGGY 1 cut(s) 710
BsrI ACTGG 3 cut(s) 670, 695, 2178
BssAI RCCGGY 1 cut(s) 710
BssMI GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
BssNI GRCGYC 3 cut(s) 423, 606, 935
BssSI CACGAG 1 cut(s) 912
BssT1I CCWWGG 1 cut(s) 1218
Bst2BI CACGAG 1 cut(s) 912
Bst2UI CCWGG 2 cut(s) 1192, 1746
Bst6I CTCTTC 1 cut(s) 155
BstACI GRCGYC 3 cut(s) 423, 606, 935
BstAPI GCANNNNNTGC 1 cut(s) 36
BstBI TTCGAA 1 cut(s) 2288
BstDEI CTNAG 3 cut(s) 437, 481, 2651
BstDSI CCRYGG 8 cut(s) 418, 715, 772, 789, 1095, 1200, 1218, 1432
BstF5I GGATG 8 cut(s) 1033, 1302, 1497, 1688, 1755, 2455, 2517, 2686
BstFNI CGCG 4 cut(s) 28, 717, 888, 1963
BstH2I RGCGCY 1 cut(s) 938
BstHHI GCGC 5 cut(s) 30, 888, 937, 1778, 2080
BstKTI GATC 9 cut(s) 801, 835, 1165, 1396, 1497, 1807, 2014, 2544, 2746
BstMAI GTCTC 2 cut(s) 491, 882
BstMBI GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
BstMCI CGRYCG 2 cut(s) 715, 1432
BstNI CCWGG 2 cut(s) 1192, 1746
BstNSI RCATGY 5 cut(s) 36, 557, 2615, 2699, 2730
BstSFI CTRYAG 2 cut(s) 1285, 1563
BstSLI GKGCMC 3 cut(s) 684, 2028, 2460
BstUI CGCG 4 cut(s) 28, 717, 888, 1963
BstV1I GCAGC 6 cut(s) 797, 968, 1498, 1691, 1900, 2000
BstV2I GAAGAC 1 cut(s) 1062
BstX2I RGATCY 2 cut(s) 1162, 2743
BstXI CCANNNNNNTGG 2 cut(s) 220, 931
BstYI RGATCY 2 cut(s) 1162, 2743
BstZI CGGCCG 1 cut(s) 712
BsuRI GGCC 7 cut(s) 640, 714, 923, 1159, 1699, 1785, 2277
BtgI CCRYGG 8 cut(s) 418, 715, 772, 789, 1095, 1200, 1218, 1432
BtgZI GCGATG 1 cut(s) 1127
BtsCI GGATG 8 cut(s) 1033, 1302, 1497, 1688, 1755, 2455, 2517, 2686
BtsI GCAGTG 1 cut(s) 855
BtsIMutI CAGTG 4 cut(s) 69, 663, 688, 855
BveI ACCTGC 1 cut(s) 215
CaiI CAGNNNCTG 1 cut(s) 2377
CfoI GCGC 5 cut(s) 30, 888, 937, 1778, 2080
Cfr10I RCCGGY 1 cut(s) 710
Cfr13I GGNCC 6 cut(s) 849, 1157, 1697, 1784, 2276, 2419
Cfr42I CCGCGG 1 cut(s) 718
Cfr9I CCCGGG 3 cut(s) 2035, 2053, 2183
CseI GACGC 2 cut(s) 412, 2356
Csp6I GTAC 6 cut(s) 93, 359, 392, 505, 958, 2146
CspCI CAANNNNNGTGG 3 cut(s) 336, 371, 2736
CviQI GTAC 6 cut(s) 93, 359, 392, 505, 958, 2146
DdeI CTNAG 3 cut(s) 437, 481, 2651
DinI GGCGCC 1 cut(s) 936
DpnI GATC 9 cut(s) 800, 834, 1164, 1395, 1496, 1806, 2013, 2543, 2745
DpnII GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
DraI TTTAAA 1 cut(s) 2718
DraIII CACNNNGTG 1 cut(s) 2464
DrdI GACNNNNNNGTC 5 cut(s) 605, 611, 632, 1183, 1846
DriI GACNNNNNGTC 1 cut(s) 1090
DseDI GACNNNNNNGTC 5 cut(s) 605, 611, 632, 1183, 1846
EaeI YGGCCR 3 cut(s) 638, 712, 921
EagI CGGCCG 1 cut(s) 712
Eam1104I CTCTTC 1 cut(s) 155
Eam1105I GACNNNNNGTC 1 cut(s) 1090
EarI CTCTTC 1 cut(s) 155
EciI GGCGGA 4 cut(s) 1040, 1132, 1550, 1915
Ecl136II GAGCTC 2 cut(s) 511, 1368
EclXI CGGCCG 1 cut(s) 712
Eco130I CCWWGG 1 cut(s) 1218
Eco24I GRGCYC 4 cut(s) 513, 546, 1370, 2047
Eco31I GGTCTC 2 cut(s) 491, 882
Eco47I GGWCC 2 cut(s) 849, 2419
Eco52I CGGCCG 1 cut(s) 712
Eco53kI GAGCTC 2 cut(s) 511, 1368
Eco57I CTGAAG 1 cut(s) 970
Eco88I CYCGRG 5 cut(s) 902, 1612, 2035, 2053, 2183
EcoICRI GAGCTC 2 cut(s) 511, 1368
EcoO109I RGGNCCY 2 cut(s) 849, 1697
EcoRII CCWGG 2 cut(s) 1190, 1744
EcoT14I CCWWGG 1 cut(s) 1218
EcoT38I GRGCYC 4 cut(s) 513, 546, 1370, 2047
EgeI GGCGCC 1 cut(s) 936
EheI GGCGCC 1 cut(s) 936
ErhI CCWWGG 1 cut(s) 1218
FalI AAGNNNNNCTT 2 cut(s) 1913, 1945
FauI CCCGC 1 cut(s) 691
FbaI TGATCA 1 cut(s) 2011
FokI GGATG 8 cut(s) 1040, 1309, 1504, 1675, 1742, 2462, 2504, 2693
FriOI GRGCYC 4 cut(s) 513, 546, 1370, 2047
FspBI CTAG 6 cut(s) 461, 1212, 1353, 1446, 1647, 2174
GlaI GCGC 5 cut(s) 29, 887, 936, 1777, 2079
GsaI CCCAGC 1 cut(s) 973
HaeII RGCGCY 1 cut(s) 938
HaeIII GGCC 7 cut(s) 640, 714, 923, 1159, 1699, 1785, 2277
HgaI GACGC 2 cut(s) 412, 2356
HhaI GCGC 5 cut(s) 30, 888, 937, 1778, 2080
Hin1I GRCGYC 3 cut(s) 423, 606, 935
Hin6I GCGC 5 cut(s) 28, 886, 935, 1776, 2078
HinP1I GCGC 5 cut(s) 28, 886, 935, 1776, 2078
HincII GTYRAC 1 cut(s) 1308
HindII GTYRAC 1 cut(s) 1308
HphI GGTGA 8 cut(s) 586, 715, 1480, 1828, 2301, 2350, 2476, 2627
Hpy166II GTNNAC 5 cut(s) 1012, 1308, 1753, 1951, 2458
Hpy188I TCNGA 9 cut(s) 560, 994, 1176, 1253, 1365, 2346, 2373, 2432, 2693
Hpy188III TCNNGA 6 cut(s) 308, 836, 1692, 2048, 2090, 2741
Hpy8I GTNNAC 5 cut(s) 1012, 1308, 1753, 1951, 2458
Hpy99I CGWCG 3 cut(s) 428, 608, 1669
HpyAV CCTTC 7 cut(s) 424, 688, 1287, 1450, 1806, 2440, 2531
HpyCH4IV ACGT 4 cut(s) 606, 1388, 1634, 2556
HpyF3I CTNAG 3 cut(s) 437, 481, 2651
HpySE526I ACGT 4 cut(s) 606, 1388, 1634, 2556
Hsp92I GRCGYC 3 cut(s) 423, 606, 935
HspAI GCGC 5 cut(s) 28, 886, 935, 1776, 2078
KasI GGCGCC 1 cut(s) 934
KflI GGGWCCC 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 2011
KspI CCGCGG 1 cut(s) 718
Kzo9I GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
LmnI GCTCC 7 cut(s) 466, 516, 698, 755, 1145, 1208, 1525
Lsp1109I GCAGC 6 cut(s) 797, 968, 1498, 1691, 1900, 2000
LweI GCATC 1 cut(s) 2526
MaeI CTAG 6 cut(s) 461, 1212, 1353, 1446, 1647, 2174
MaeII ACGT 4 cut(s) 606, 1388, 1634, 2556
MaeIII GTNAC 4 cut(s) 582, 592, 2263, 2633
MalI GATC 9 cut(s) 800, 834, 1164, 1395, 1496, 1806, 2013, 2543, 2745
MbiI CCGCTC 4 cut(s) 457, 1019, 1866, 1877
MboI GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
MflI RGATCY 2 cut(s) 1162, 2743
MhlI GDGCHC 7 cut(s) 513, 546, 684, 1370, 2028, 2047, 2460
Mly113I GGCGCC 1 cut(s) 935
MlyI GAGTC 7 cut(s) 342, 486, 535, 600, 1041, 1915, 1973
MmeI TCCRAC 4 cut(s) 367, 733, 1186, 1371
MroXI GAANNNNTTC 2 cut(s) 413, 1345
MseI TTAA 4 cut(s) 21, 231, 819, 2717
MslI CAYNNNNRTG 2 cut(s) 668, 777
MspA1I CMGCKG 3 cut(s) 717, 1511, 1802
MvaI CCWGG 2 cut(s) 1192, 1746
MvnI CGCG 4 cut(s) 28, 717, 888, 1963
NarI GGCGCC 1 cut(s) 935
NcoI CCATGG 1 cut(s) 1218
NdeII GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
NheI GCTAGC 1 cut(s) 1211
NlaIV GGNNCC 8 cut(s) 681, 850, 851, 936, 1164, 1743, 1895, 2421
NmeAIII GCCGAG 5 cut(s) 478, 852, 963, 1424, 1964
NmuCI GTSAC 4 cut(s) 582, 592, 2263, 2633
NspI RCATGY 5 cut(s) 36, 557, 2615, 2699, 2730
NspV TTCGAA 1 cut(s) 2288
PciI ACATGT 3 cut(s) 32, 2611, 2726
PdmI GAANNNNTTC 2 cut(s) 413, 1345
PfeI GAWTC 4 cut(s) 1587, 1720, 2606, 2737
PflFI GACNNNGTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 579
PfoI TCCNGGA 3 cut(s) 1835, 2220, 2421
PleI GAGTC 7 cut(s) 341, 485, 534, 599, 1040, 1914, 1973
PluTI GGCGCC 1 cut(s) 938
PpsI GAGTC 7 cut(s) 341, 485, 534, 599, 1040, 1914, 1973
PpuMI RGGWCCY 1 cut(s) 849
PscI ACATGT 3 cut(s) 32, 2611, 2726
Psp124BI GAGCTC 2 cut(s) 513, 1370
Psp1406I AACGTT 1 cut(s) 1634
Psp5II RGGWCCY 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 1190, 1744
PspFI CCCAGC 1 cut(s) 969
PspGI CCWGG 2 cut(s) 1190, 1744
PspN4I GGNNCC 8 cut(s) 681, 850, 851, 936, 1164, 1743, 1895, 2421
PspPI GGNCC 6 cut(s) 849, 1157, 1697, 1784, 2276, 2419
PspPPI RGGWCCY 1 cut(s) 849
PstNI CAGNNNCTG 1 cut(s) 2377
PsuI RGATCY 2 cut(s) 1162, 2743
PsyI GACNNNGTC 1 cut(s) 476
PvuII CAGCTG 1 cut(s) 1511
RsaI GTAC 6 cut(s) 94, 360, 393, 506, 959, 2147
RsaNI GTAC 6 cut(s) 93, 359, 392, 505, 958, 2146
RseI CAYNNNNRTG 2 cut(s) 668, 777
SacI GAGCTC 2 cut(s) 513, 1370
SacII CCGCGG 1 cut(s) 718
SaqAI TTAA 4 cut(s) 21, 231, 819, 2717
Sau3AI GATC 9 cut(s) 798, 832, 1162, 1393, 1494, 1804, 2011, 2541, 2743
Sau96I GGNCC 6 cut(s) 849, 1157, 1697, 1784, 2276, 2419
ScaI AGTACT 1 cut(s) 2147
SchI GAGTC 7 cut(s) 342, 486, 535, 600, 1041, 1915, 1973
SduI GDGCHC 7 cut(s) 513, 546, 684, 1370, 2028, 2047, 2460
SfaNI GCATC 1 cut(s) 2526
SfcI CTRYAG 2 cut(s) 1285, 1563
SfoI GGCGCC 1 cut(s) 936
Sfr303I CCGCGG 1 cut(s) 718
SfuI TTCGAA 1 cut(s) 2288
SgrBI CCGCGG 1 cut(s) 718
SinI GGWCC 2 cut(s) 849, 2419
SmaI CCCGGG 3 cut(s) 2037, 2055, 2185
SmiMI CAYNNNNRTG 2 cut(s) 668, 777
SmlI CTYRAG 3 cut(s) 1715, 1724, 1883
SmoI CTYRAG 3 cut(s) 1715, 1724, 1883
SpeI ACTAGT 1 cut(s) 1352
SspDI GGCGCC 1 cut(s) 934
SspMI CTAG 6 cut(s) 461, 1212, 1353, 1446, 1647, 2174
SstI GAGCTC 2 cut(s) 513, 1370
StyI CCWWGG 1 cut(s) 1218
TaiI ACGT 4 cut(s) 609, 1391, 1637, 2559
TaqI TCGA 3 cut(s) 546, 2288, 2337
TatI WGTACW 1 cut(s) 2145
TfiI GAWTC 4 cut(s) 1587, 1720, 2606, 2737
Tru1I TTAA 4 cut(s) 21, 231, 819, 2717
Tru9I TTAA 4 cut(s) 21, 231, 819, 2717
TscAI CASTG 4 cut(s) 76, 670, 695, 862
TseFI GTSAC 4 cut(s) 582, 592, 2263, 2633
TseI GCWGC 6 cut(s) 785, 981, 1511, 1679, 1913, 1988
Tsp45I GTSAC 4 cut(s) 582, 592, 2263, 2633
TspDTI ATGAA 7 cut(s) 17, 210, 236, 1313, 1677, 2565, 2619
TspGWI ACGGA 5 cut(s) 489, 789, 827, 1084, 1533
TspMI CCCGGG 3 cut(s) 2035, 2053, 2183
TspRI CASTG 4 cut(s) 76, 670, 695, 862
Tth111I GACNNNGTC 1 cut(s) 476
Van91I CCANNNNNTGG 1 cut(s) 579
VneI GTGCAC 1 cut(s) 2456
VpaK11BI GGWCC 2 cut(s) 849, 2419
XapI RAATTY 6 cut(s) 762, 1125, 2093, 2354, 2498, 2713
XceI RCATGY 5 cut(s) 36, 557, 2615, 2699, 2730
XmaI CCCGGG 3 cut(s) 2035, 2053, 2183
XmnI GAANNNNTTC 2 cut(s) 413, 1345
XspI CTAG 6 cut(s) 461, 1212, 1353, 1446, 1647, 2174
ZraI GACGTC 1 cut(s) 607
ZrmI AGTACT 1 cut(s) 2147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.