Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Contig00028

Orthogroup ID

OG0013557

Physical Location & Seq

Genomic Coordinates Reverse (-)

33 .. 2180

Gene Structure 2148 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw0G000960.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2085 bp

ATGGTTCATGTTCAAAAACCAGTGATCAAATGGCTGGTTCTGATTCTAACAAGCTCTGTCTTCTTCAGCATGGCCCTCTCTGCAAAAACCCAAACTTATATCATCCAAATGGACAAGTCCGCCAAGCCTGAATCCTTCACCACCCACCTCGATTGGTACTCATCCAAAGTAAACTCAGTACTCAACAAACCAGAAAAAGGAGACGATCAAAACCAAGACAGAATCATTTACACTTACCAAACCGCCTTTCATGGCGTCGCAGCTCAACTGAGCAAACAAGAAGCAGACCTACTAGAGCAACAAGACGGAGTCTTGGCTATATTCCCAGAACAGAAGTACCAGCTCCACACCACCAGAAGTCCTCTGTTTCTCGGGCTCGAACCACACGACAGCACCACCGACAACGTCTGGTCCCAGCGAGTCTCCGACCACGACGTCATCGTAGGAGTACTCGACACGGGAGTCTGGCCGGAGAGCCAGAGCTTCAACGACACCGGAATGTCTCCGGTTCCGCGGCGCTGGCGCGGCGCATGCGAGACCGGCCGCGGCTTCACAAAAAGCAACTGCAACAGGAAAATCGTCGGCGCCAGAATCTTCCACCGGGGTACGAGTCCGCCACCGGAAAAATCAACGAGAAGACGGAGTACAAGTCGCCGAGGGACCAGGACGGACACGGGACCCACACCGCCGCAACCGTCGCCGGATCTCCCGTACGTGGCGCCAATCTTCTCGGCTACGCGTACGGTACGGCAAGAGGAATGGCGCCGGCGGCGAGGATCGCGGTGTACAAGGACGGTGTCGATGTTTTGTCTATTTCTCTTGGCGGTGGAGTTTCCTCGTATTACCGGGATAGTTGTCTATTGCGGCGTTTGGTGCGATGGAGATGGGTGTTTTTGTCTCTGCTCTGCCGGGAATGGTGGGCCAGACCCGGTGAGTCTGACTAATGTGTCGCCTTGGATCACCACCGTCGGTGCTAGCACTATGGATAGGGATTTTCCGGCCACTGTGAAGTTGGGGAATGGGAGGACTATTACTGGAGTTTCGCTTTATAGAGGGAGAATGAAGCTTGACGCAAAGAAGCTGTACCCTGTTGTGTTAGCGGGTGGGAATTCGAGTAGCCCCAATCCGAGCTCGTTGTGTTTAGAGGGTACTTTAGATCCGAAGAAAGTTAAGGGGAAGATTGTGATTTGTGATCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGACGTGGTGAAGAATGCCGGAGGAGTTGGGATGATCTTGGCTAATACGGCTTCGAATGGGGAGGAGCTGGTTGCTGATTGTCATCTGATTCCGGCGGTTGCGGTGGGAGAGAATGAAGCAAAATCGATCAAGCATTACGCCTTAACAAGTCCGAAAGCAAAGGCAACTCTAGCATTTGTTGGTACTAGACTAGGGGTTAGACCGTCTCCCATTGTGGCTGCATTTTCGTCAAGAGGGCCTAATCTTTTGAGTTTTGAGATTATGAAGCCGGATTTGGTGGCGCCGGGAGTGAACATTCTTGCTGCTTGGACTGGGGCATTGGGTCCGTCGAGCTTGGCAACGGATCGCAGGAGAGTGAAGTTCAATATCTTGTCCGGGACTTCAATGTCGTGTCCTCATGTGAGTGGGATTGCTGCTTTGCTCAAATCTAGGCACCCCGAATGGAGTCCAGCCGCGATCAAATCCGCCTTGATGACTACAGCTTATGTTCATGACAACACACATAAGCCTCTGGAAGATGCCTCAACAGCTAAAATTTCAACCCCTTTTGATCATGGAGCTGGACACATAAACCCGAGGAAGGCTCTTGACCCGGGCTTGGTTTATGACATTCAGCCCCAGGATTATCTTGAGTTCCTCTGCACACAAAAACTAACCCCAACGCAGCTCAAGGTGTTTAGCAATAGCACTTGCCGTCACACTCTGGCCAATGCAGGGGACCTCAACTATCCGGCAATTTCCGTTGTGTTTGTGGAGAACACTAATGTCTCTGTCTTGACAGTTCATAGAACTGTCACTAATGTTGGCCCTGCGGTTTCGAGTTACCATGCCATGTTTCGCCATTCAAGGGTGCTTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004175 GO:0004252 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008236 GO:0016787 GO:0017171 GO:0019538 GO:0043170 GO:0044238 GO:0070011 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I9 PA Peptidase_S8

Protein Analysis

694

Amino Acids

75.62

Weight (kDa)

9.44

Isoelectric Point (pI)

43.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I9	PF05922	32 - 116	1.3e-20	Peptidase inhibitor I9
Peptidase_S8	PF00082	297 - 575	8.3e-24	Subtilase family
PA	PF02225	362 - 451	1.5e-13	PA domain
fn3_6	PF17766	648 - 689	7e-07	Fibronectin type-III domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013557)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G51750
fragaria_vesca	FvH4_7g28501
malus_domestica	MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica	Prupe.2G285500_v2.0.a1
pyrus_communis	pycom01g20050
rosa_chinensis	RchiOBHm_Chr1g0375891
rosa_laevigata	RLG00000026646
rosa_multiflora	Rmu_sc0001943.1_g000010
rosa_roxburghii	Rroxscaffold_4G00282550
rosa_rugosa	Rorug01G0392200
rosa_samantha	Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana	Rw0G000960

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	4 cut(s)	434, 461, 946, 1609
AatII	GACGTC	1 cut(s)	438
AccB1I	GGYRCC	5 cut(s)	584, 718, 762, 1504, 1656
AccII	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
AclWI	GGATC	5 cut(s)	711, 784, 965, 1151, 1575
AcoI	YGGCCR	4 cut(s)	467, 541, 999, 1929
AcsI	RAATTY	2 cut(s)	1108, 1758
AcuI	CTGAAG	1 cut(s)	49
AcyI	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
AfiI	CCNNNNNNNGG	6 cut(s)	197, 715, 1804, 1822, 1938, 2071
AflIII	ACRYGT	1 cut(s)	737
AgsI	TTSAA	6 cut(s)	14, 487, 1588, 1608, 1764, 2070
AjiI	CACGTC	1 cut(s)	1228
AjnI	CCWGG	2 cut(s)	662, 1842
AjuI	GAANNNNNNNTTGG	4 cut(s)	231, 263, 1481, 1513
Alw21I	GWGCWC	1 cut(s)	1133
Alw26I	GTCTC	8 cut(s)	195, 427, 507, 530, 902, 1218, 1434, 1996
AlwI	GGATC	5 cut(s)	711, 784, 965, 1151, 1575
Ama87I	CYCGRG	3 cut(s)	371, 1798, 1816
AoxI	GGCC	8 cut(s)	72, 467, 541, 920, 999, 1460, 1929, 2029
ApeKI	GCWGC	5 cut(s)	260, 1442, 1526, 1637, 1888
ApoI	RAATTY	2 cut(s)	1108, 1758
AspLEI	GCGC	7 cut(s)	519, 525, 530, 587, 721, 765, 1507
AspS9I	GGNCC	9 cut(s)	73, 411, 660, 677, 920, 1460, 1547, 1942, 2030
AsuC2I	CCSGG	8 cut(s)	602, 847, 910, 929, 1509, 1600, 1817, 1818
AsuHPI	GGTGA	4 cut(s)	130, 943, 952, 1243
AsuII	TTCGAA	1 cut(s)	1277
AsuNHI	GCTAGC	1 cut(s)	974
AvaI	CYCGRG	3 cut(s)	371, 1798, 1816
AvaII	GGWCC	5 cut(s)	411, 660, 677, 1547, 1942
BaeI	ACNNNNGTAYC	2 cut(s)	320, 353
BalI	TGGCCA	1 cut(s)	1931
BanI	GGYRCC	5 cut(s)	584, 718, 762, 1504, 1656
BanII	GRGCYC	2 cut(s)	378, 1133
BarI	GAAGNNNNNNTAC	4 cut(s)	273, 305, 628, 660
BbsI	GAAGAC	2 cut(s)	52, 643
Bbv12I	GWGCWC	1 cut(s)	1133
BbvI	GCAGC	5 cut(s)	272, 1429, 1513, 1624, 1900
BccI	CCATC	2 cut(s)	872, 878
BceAI	ACGGC	3 cut(s)	764, 1287, 1902
BciT130I	CCWGG	2 cut(s)	664, 1844
BclI	TGATCA	2 cut(s)	24, 1774
BcnI	CCSGG	8 cut(s)	602, 847, 910, 929, 1509, 1600, 1817, 1818
BcoDI	GTCTC	8 cut(s)	195, 427, 507, 530, 902, 1218, 1434, 1996
BfaI	CTAG	7 cut(s)	293, 975, 1209, 1394, 1410, 1415, 1653
BfmI	CTRYAG	1 cut(s)	1701
BfoI	RGCGCY	5 cut(s)	520, 588, 722, 766, 1508
BmcAI	AGTACT	2 cut(s)	180, 450
Bme18I	GGWCC	5 cut(s)	411, 660, 677, 1547, 1942
BmeT110I	CYCGRG	3 cut(s)	371, 1798, 1816
BmgBI	CACGTC	1 cut(s)	1228
BmgT120I	GGNCC	9 cut(s)	73, 411, 660, 677, 920, 1460, 1547, 1942, 2030
BmrI	ACTGGG	1 cut(s)	1545
BmsI	GCATC	1 cut(s)	1732
BmtI	GCTAGC	1 cut(s)	978
BmuI	ACTGGG	1 cut(s)	1545
BpiI	GAAGAC	2 cut(s)	52, 643
BplI	GAGNNNNNCTC	2 cut(s)	1792, 1824
BpmI	CTGGAG	1 cut(s)	1056
Bpu14I	TTCGAA	1 cut(s)	1277
BpuEI	CTTGAG	2 cut(s)	1874, 1877
BpuMI	CCSGG	8 cut(s)	602, 847, 910, 929, 1509, 1600, 1817, 1818
Bsa29I	ATCGAT	1 cut(s)	1349
BsaAI	YACGTR	1 cut(s)	715
BsaBI	GATNNNNATC	1 cut(s)	1305
BsaHI	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
BsaI	GGTCTC	1 cut(s)	530
BsaJI	CCNNGG	8 cut(s)	512, 544, 601, 655, 953, 1799, 1816, 1842
BsaWI	WCCGGW	3 cut(s)	494, 505, 619
BsaXI	ACNNNNNCTCC	4 cut(s)	634, 664, 1215, 1245
Bsc4I	CCNNNNNNNGG	6 cut(s)	197, 715, 1804, 1822, 1938, 2071
Bse118I	RCCGGY	2 cut(s)	539, 765
Bse1I	ACTGG	3 cut(s)	20, 1039, 1540
Bse8I	GATNNNNATC	1 cut(s)	1305
BseBI	CCWGG	2 cut(s)	664, 1844
BseCI	ATCGAT	1 cut(s)	1349
BseDI	CCNNGG	8 cut(s)	512, 544, 601, 655, 953, 1799, 1816, 1842
BseGI	GGATG	3 cut(s)	102, 161, 1260
BseJI	GATNNNNATC	1 cut(s)	1305
BseLI	CCNNNNNNNGG	6 cut(s)	197, 715, 1804, 1822, 1938, 2071
BseMII	CTCAG	2 cut(s)	189, 260
BseNI	ACTGG	3 cut(s)	20, 1039, 1540
BseRI	GAGGAG	2 cut(s)	1260, 1301
BseX3I	CGGCCG	1 cut(s)	541
BseXI	GCAGC	5 cut(s)	272, 1429, 1513, 1624, 1900
BseYI	CCCAGC	1 cut(s)	414
BsgI	GTGCAG	2 cut(s)	1235, 1849
Bsh1236I	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
Bsh1285I	CGRYCG	1 cut(s)	544
BshFI	GGCC	8 cut(s)	74, 469, 543, 922, 1001, 1462, 1931, 2031
BshNI	GGYRCC	5 cut(s)	584, 718, 762, 1504, 1656
BshVI	ATCGAT	1 cut(s)	1349
BsiEI	CGRYCG	1 cut(s)	544
BsiHKAI	GWGCWC	1 cut(s)	1133
BsiHKCI	CYCGRG	3 cut(s)	371, 1798, 1816
BsiWI	CGTACG	2 cut(s)	711, 740
BslFI	GGGAC	5 cut(s)	397, 673, 690, 1615, 1955
BslI	CCNNNNNNNGG	6 cut(s)	197, 715, 1804, 1822, 1938, 2071
BsmAI	GTCTC	8 cut(s)	195, 427, 507, 530, 902, 1218, 1434, 1996
BsmBI	CGTCTC	3 cut(s)	195, 1218, 1434
BsmFI	GGGAC	5 cut(s)	397, 673, 690, 1615, 1955
BsmI	GAATGC	1 cut(s)	1243
BsnI	GGCC	8 cut(s)	74, 469, 543, 922, 1001, 1462, 1931, 2031
Bso31I	GGTCTC	1 cut(s)	530
BsoBI	CYCGRG	3 cut(s)	371, 1798, 1816
Bsp119I	TTCGAA	1 cut(s)	1277
Bsp1286I	GDGCHC	2 cut(s)	378, 1133
Bsp1407I	TGTACA	1 cut(s)	785
BspANI	GGCC	8 cut(s)	74, 469, 543, 922, 1001, 1462, 1931, 2031
BspCNI	CTCAG	2 cut(s)	188, 261
BspDI	ATCGAT	1 cut(s)	1349
BspFNI	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
BspHI	TCATGA	1 cut(s)	1714
BspOI	GCTAGC	1 cut(s)	978
BspPI	GGATC	5 cut(s)	711, 784, 965, 1151, 1575
BspT104I	TTCGAA	1 cut(s)	1277
BspT107I	GGYRCC	5 cut(s)	584, 718, 762, 1504, 1656
BspTNI	GGTCTC	1 cut(s)	530
BsrFI	RCCGGY	2 cut(s)	539, 765
BsrGI	TGTACA	1 cut(s)	785
BsrI	ACTGG	3 cut(s)	20, 1039, 1540
BssAI	RCCGGY	2 cut(s)	539, 765
BssECI	CCNNGG	8 cut(s)	512, 544, 601, 655, 953, 1799, 1816, 1842
BssNI	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
BssT1I	CCWWGG	1 cut(s)	953
Bst2UI	CCWGG	2 cut(s)	664, 1844
Bst4CI	ACNGT	8 cut(s)	696, 745, 796, 967, 1006, 1428, 2005, 2017
BstACI	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
BstAUI	TGTACA	1 cut(s)	785
BstBAI	YACGTR	1 cut(s)	715
BstBI	TTCGAA	1 cut(s)	1277
BstC8I	GCNNGC	4 cut(s)	521, 532, 767, 976
BstDEI	CTNAG	2 cut(s)	175, 269
BstDSI	CCRYGG	2 cut(s)	512, 544
BstF5I	GGATG	3 cut(s)	102, 161, 1260
BstFNI	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
BstH2I	RGCGCY	5 cut(s)	520, 588, 722, 766, 1508
BstHHI	GCGC	7 cut(s)	519, 525, 530, 587, 721, 765, 1507
BstMAI	GTCTC	8 cut(s)	195, 427, 507, 530, 902, 1218, 1434, 1996
BstMCI	CGRYCG	1 cut(s)	544
BstNI	CCWGG	2 cut(s)	664, 1844
BstNSI	RCATGY	1 cut(s)	534
BstSFI	CTRYAG	1 cut(s)	1701
BstUI	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
BstV1I	GCAGC	5 cut(s)	272, 1429, 1513, 1624, 1900
BstV2I	GAAGAC	2 cut(s)	52, 643
BstX2I	RGATCY	2 cut(s)	703, 1156
BstYI	RGATCY	2 cut(s)	703, 1156
BstZI	CGGCCG	1 cut(s)	541
Bsu15I	ATCGAT	1 cut(s)	1349
BsuRI	GGCC	8 cut(s)	74, 469, 543, 922, 1001, 1462, 1931, 2031
BsuTUI	ATCGAT	1 cut(s)	1349
BtgI	CCRYGG	2 cut(s)	512, 544
BtgZI	GCGATG	1 cut(s)	891
BtrI	CACGTC	1 cut(s)	1228
BtsCI	GGATG	3 cut(s)	102, 161, 1260
BtsIMutI	CAGTG	2 cut(s)	27, 1002
Cac8I	GCNNGC	4 cut(s)	521, 532, 767, 976
CciI	TCATGA	1 cut(s)	1714
CfoI	GCGC	7 cut(s)	519, 525, 530, 587, 721, 765, 1507
Cfr10I	RCCGGY	2 cut(s)	539, 765
Cfr13I	GGNCC	9 cut(s)	73, 411, 660, 677, 920, 1460, 1547, 1942, 2030
Cfr42I	CCGCGG	2 cut(s)	515, 547
Cfr9I	CCCGGG	1 cut(s)	1816
ClaI	ATCGAT	1 cut(s)	1349
CseI	GACGC	2 cut(s)	244, 1079
CspCI	CAANNNNNGTGG	2 cut(s)	134, 169
CviAII	CATG	9 cut(s)	8, 70, 251, 531, 1622, 1715, 1778, 2051, 2056
DdeI	CTNAG	2 cut(s)	175, 269
DinI	GGCGCC	4 cut(s)	586, 720, 764, 1506
DrdI	GACNNNNNNGTC	4 cut(s)	434, 461, 946, 1609
DseDI	GACNNNNNNGTC	4 cut(s)	434, 461, 946, 1609
EaeI	YGGCCR	4 cut(s)	467, 541, 999, 1929
EagI	CGGCCG	1 cut(s)	541
EciI	GGCGGA	3 cut(s)	109, 603, 1678
Ecl136II	GAGCTC	1 cut(s)	1131
EclXI	CGGCCG	1 cut(s)	541
Eco130I	CCWWGG	1 cut(s)	953
Eco24I	GRGCYC	2 cut(s)	378, 1133
Eco31I	GGTCTC	1 cut(s)	530
Eco47I	GGWCC	5 cut(s)	411, 660, 677, 1547, 1942
Eco52I	CGGCCG	1 cut(s)	541
Eco53kI	GAGCTC	1 cut(s)	1131
Eco57I	CTGAAG	1 cut(s)	49
Eco88I	CYCGRG	3 cut(s)	371, 1798, 1816
EcoICRI	GAGCTC	1 cut(s)	1131
EcoO109I	RGGNCCY	3 cut(s)	677, 1460, 1942
EcoRI	GAATTC	1 cut(s)	1108
EcoRII	CCWGG	2 cut(s)	662, 1842
EcoT14I	CCWWGG	1 cut(s)	953
EcoT38I	GRGCYC	2 cut(s)	378, 1133
EgeI	GGCGCC	4 cut(s)	586, 720, 764, 1506
EheI	GGCGCC	4 cut(s)	586, 720, 764, 1506
ErhI	CCWWGG	1 cut(s)	953
Esp3I	CGTCTC	3 cut(s)	195, 1218, 1434
FaeI	CATG	9 cut(s)	11, 73, 254, 534, 1625, 1718, 1781, 2054, 2059
FaqI	GGGAC	5 cut(s)	397, 673, 690, 1615, 1955
FatI	CATG	9 cut(s)	7, 69, 250, 530, 1621, 1714, 1777, 2050, 2055
FauI	CCCGC	1 cut(s)	1093
FbaI	TGATCA	2 cut(s)	24, 1774
FokI	GGATG	3 cut(s)	89, 148, 1267
FriOI	GRGCYC	2 cut(s)	378, 1133
FspBI	CTAG	7 cut(s)	293, 975, 1209, 1394, 1410, 1415, 1653
GlaI	GCGC	7 cut(s)	518, 524, 529, 586, 720, 764, 1506
GsaI	CCCAGC	1 cut(s)	418
GsuI	CTGGAG	1 cut(s)	1056
HaeII	RGCGCY	5 cut(s)	520, 588, 722, 766, 1508
HaeIII	GGCC	8 cut(s)	74, 469, 543, 922, 1001, 1462, 1931, 2031
HgaI	GACGC	2 cut(s)	244, 1079
HhaI	GCGC	7 cut(s)	519, 525, 530, 587, 721, 765, 1507
Hin1I	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
Hin1II	CATG	9 cut(s)	11, 73, 254, 534, 1625, 1718, 1781, 2054, 2059
Hin6I	GCGC	7 cut(s)	517, 523, 528, 585, 719, 763, 1505
HinP1I	GCGC	7 cut(s)	517, 523, 528, 585, 719, 763, 1505
HindIII	AAGCTT	1 cut(s)	1064
HphI	GGTGA	4 cut(s)	130, 943, 952, 1243
Hpy166II	GTNNAC	3 cut(s)	172, 786, 1516
Hpy188I	TCNGA	7 cut(s)	42, 427, 939, 1128, 1161, 1311, 1377
Hpy188III	TCNNGA	6 cut(s)	1455, 1715, 1736, 1811, 1853, 1999
Hpy8I	GTNNAC	3 cut(s)	172, 786, 1516
Hpy99I	CGWCG	6 cut(s)	260, 437, 584, 700, 971, 1555
HpyAV	CCTTC	3 cut(s)	145, 1213, 1798
HpyCH4III	ACNGT	8 cut(s)	696, 745, 796, 967, 1006, 1428, 2005, 2017
HpyCH4IV	ACGT	4 cut(s)	405, 435, 714, 1227
HpyCH4V	TGCA	6 cut(s)	83, 567, 1216, 1445, 1866, 1937
HpyF3I	CTNAG	2 cut(s)	175, 269
HpySE526I	ACGT	4 cut(s)	405, 435, 714, 1227
Hsp92I	GRCGYC	6 cut(s)	255, 435, 585, 719, 763, 1505
Hsp92II	CATG	9 cut(s)	11, 73, 254, 534, 1625, 1718, 1781, 2054, 2059
HspAI	GCGC	7 cut(s)	517, 523, 528, 585, 719, 763, 1505
KasI	GGCGCC	4 cut(s)	584, 718, 762, 1504
KflI	GGGWCCC	1 cut(s)	677
KroI	GCCGGC	1 cut(s)	765
KroNI	GCCGGC	1 cut(s)	767
Ksp22I	TGATCA	2 cut(s)	24, 1774
KspI	CCGCGG	2 cut(s)	515, 547
LmnI	GCTCC	3 cut(s)	348, 1288, 1781
Lsp1109I	GCAGC	5 cut(s)	272, 1429, 1513, 1624, 1900
LweI	GCATC	1 cut(s)	1732
MaeI	CTAG	7 cut(s)	293, 975, 1209, 1394, 1410, 1415, 1653
MaeII	ACGT	4 cut(s)	405, 435, 714, 1227
MaeIII	GTNAC	3 cut(s)	1919, 2017, 2045
MboII	GAAGA	9 cut(s)	52, 55, 586, 648, 718, 1174, 1189, 1246, 1751
MflI	RGATCY	2 cut(s)	703, 1156
MhlI	GDGCHC	2 cut(s)	378, 1133
MlsI	TGGCCA	1 cut(s)	1931
MluCI	AATT	3 cut(s)	1108, 1758, 1959
MluI	ACGCGT	1 cut(s)	737
MluNI	TGGCCA	1 cut(s)	1931
Mly113I	GGCGCC	4 cut(s)	585, 719, 763, 1505
MlyI	GAGTC	6 cut(s)	318, 429, 471, 619, 943, 1678
MmeI	TCCRAC	1 cut(s)	450
Mox20I	TGGCCA	1 cut(s)	1931
MreI	CGCCGGCG	1 cut(s)	765
MroNI	GCCGGC	1 cut(s)	765
MscI	TGGCCA	1 cut(s)	1931
MseI	TTAA	2 cut(s)	1170, 1367
MslI	CAYNNNNRTG	3 cut(s)	107, 497, 1626
Msp20I	TGGCCA	1 cut(s)	1931
MspA1I	CMGCKG	2 cut(s)	514, 546
MteI	GCGCNGCGC	1 cut(s)	526
Mva1269I	GAATGC	1 cut(s)	1243
MvaI	CCWGG	2 cut(s)	664, 1844
MvnI	CGCG	6 cut(s)	514, 525, 546, 739, 781, 1679
NaeI	GCCGGC	1 cut(s)	767
NarI	GGCGCC	4 cut(s)	585, 719, 763, 1505
NciI	CCSGG	8 cut(s)	602, 847, 910, 929, 1509, 1600, 1817, 1818
NgoMIV	GCCGGC	1 cut(s)	765
NheI	GCTAGC	1 cut(s)	974
NlaIII	CATG	9 cut(s)	11, 73, 254, 534, 1625, 1718, 1781, 2054, 2059
NmeAIII	GCCGAG	2 cut(s)	680, 710
NmuCI	GTSAC	2 cut(s)	1919, 2017
NspI	RCATGY	1 cut(s)	534
NspV	TTCGAA	1 cut(s)	1277
PaeI	GCATGC	1 cut(s)	534
PagI	TCATGA	1 cut(s)	1714
PcsI	WCGNNNNNNNCGW	2 cut(s)	384, 438
PctI	GAATGC	1 cut(s)	1243
PdiI	GCCGGC	1 cut(s)	767
PfeI	GAWTC	5 cut(s)	43, 131, 222, 591, 1312
Pfl23II	CGTACG	2 cut(s)	711, 740
PflFI	GACNNNGTC	3 cut(s)	308, 404, 796
PfoI	TCCNGGA	1 cut(s)	1598
PleI	GAGTC	6 cut(s)	317, 428, 470, 618, 942, 1677
PluTI	GGCGCC	4 cut(s)	588, 722, 766, 1508
PpsI	GAGTC	6 cut(s)	317, 428, 470, 618, 942, 1677
Ppu21I	YACGTR	1 cut(s)	715
PpuMI	RGGWCCY	2 cut(s)	677, 1942
Psp124BI	GAGCTC	1 cut(s)	1133
Psp5II	RGGWCCY	2 cut(s)	677, 1942
Psp6I	CCWGG	2 cut(s)	662, 1842
PspFI	CCCAGC	1 cut(s)	414
PspGI	CCWGG	2 cut(s)	662, 1842
PspLI	CGTACG	2 cut(s)	711, 740
PspPI	GGNCC	9 cut(s)	73, 411, 660, 677, 920, 1460, 1547, 1942, 2030
PspPPI	RGGWCCY	2 cut(s)	677, 1942
PsuI	RGATCY	2 cut(s)	703, 1156
PsyI	GACNNNGTC	3 cut(s)	308, 404, 796
RseI	CAYNNNNRTG	3 cut(s)	107, 497, 1626
SacI	GAGCTC	1 cut(s)	1133
SacII	CCGCGG	2 cut(s)	515, 547
SaqAI	TTAA	2 cut(s)	1170, 1367
Sau96I	GGNCC	9 cut(s)	73, 411, 660, 677, 920, 1460, 1547, 1942, 2030
ScaI	AGTACT	2 cut(s)	180, 450
SchI	GAGTC	6 cut(s)	318, 429, 471, 619, 943, 1678
SduI	GDGCHC	2 cut(s)	378, 1133
SfaNI	GCATC	1 cut(s)	1732
SfcI	CTRYAG	1 cut(s)	1701
SfoI	GGCGCC	4 cut(s)	586, 720, 764, 1506
Sfr303I	CCGCGG	2 cut(s)	515, 547
SfuI	TTCGAA	1 cut(s)	1277
SgrAI	CRCCGGYG	1 cut(s)	765
SgrBI	CCGCGG	2 cut(s)	515, 547
SinI	GGWCC	5 cut(s)	411, 660, 677, 1547, 1942
SmaI	CCCGGG	1 cut(s)	1818
SmiMI	CAYNNNNRTG	3 cut(s)	107, 497, 1626
SmlI	CTYRAG	2 cut(s)	1853, 1892
SmoI	CTYRAG	2 cut(s)	1853, 1892
SphI	GCATGC	1 cut(s)	534
Sse9I	AATT	3 cut(s)	1108, 1758, 1959
SspDI	GGCGCC	4 cut(s)	584, 718, 762, 1504
SspMI	CTAG	7 cut(s)	293, 975, 1209, 1394, 1410, 1415, 1653
SstI	GAGCTC	1 cut(s)	1133
StyI	CCWWGG	1 cut(s)	953
TaaI	ACNGT	8 cut(s)	696, 745, 796, 967, 1006, 1428, 2005, 2017
TaiI	ACGT	4 cut(s)	408, 438, 717, 1230
TaqI	TCGA	9 cut(s)	150, 378, 453, 800, 1112, 1277, 1349, 1553, 2042
TasI	AATT	3 cut(s)	1108, 1758, 1959
TatI	WGTACW	4 cut(s)	178, 448, 644, 785
TauI	GCSGC	8 cut(s)	517, 528, 546, 549, 691, 772, 867, 1679
TfiI	GAWTC	5 cut(s)	43, 131, 222, 591, 1312
Tru1I	TTAA	2 cut(s)	1170, 1367
Tru9I	TTAA	2 cut(s)	1170, 1367
TscAI	CASTG	2 cut(s)	27, 1009
TseFI	GTSAC	2 cut(s)	1919, 2017
TseI	GCWGC	5 cut(s)	260, 1442, 1526, 1637, 1888
Tsp45I	GTSAC	2 cut(s)	1919, 2017
TspDTI	ATGAA	6 cut(s)	239, 1076, 1353, 1502, 1703, 1997
TspGWI	ACGGA	6 cut(s)	321, 655, 683, 1539, 1580, 1954
TspMI	CCCGGG	1 cut(s)	1816
TspRI	CASTG	2 cut(s)	27, 1009
Tth111I	GACNNNGTC	3 cut(s)	308, 404, 796
VpaK11BI	GGWCC	5 cut(s)	411, 660, 677, 1547, 1942
XapI	RAATTY	2 cut(s)	1108, 1758
XceI	RCATGY	1 cut(s)	534
XcmI	CCANNNNNNNNNTGG	2 cut(s)	27, 1009
XmaI	CCCGGG	1 cut(s)	1816
XspI	CTAG	7 cut(s)	293, 975, 1209, 1394, 1410, 1415, 1653
ZraI	GACGTC	1 cut(s)	436
ZrmI	AGTACT	2 cut(s)	180, 450

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.