MD01G1189900.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013557
Physical Location & Seq
Forward (+)
28886302 .. 28887624
1323 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1189900.v1.1.491

Sequence Viewer

Length: 1323 bp
ATGGTAGAGAAAAGTACTACGGTGGGAGCTAGCACCATGGATAGAGATTTTCCAGCCACTGTGAAGCTCGGAAATGGCAGGACTATTACCGGGGTTTCGCTCTACAGGGGAAGGATGATGCTGTCAACAAACAAGCAATACCCTGTTGTGTATTTGGGAAGCAATTCAACTAGTCCTAATCCGAGCTCGCTGTGTTTGGAGGGGACGTTGGATCGCCGAGTTGTGGCCGGAAAGATTGTGATTTGCGACCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGAGGTGGTGAAAGAAGCCGGAGGAGTTGGGATGATCTTGGCAAACACAGCTGCAAACGGAGAGGAGCTTGTGGCGGATTGCCACCTTGTTCCGGCGGTTGCTGTGGGAGAGAACGAAGCAAAGGGAATCAAGCATTATGCTTCAACAAGCCCGAGAGCCACCGCAACTCTAACATTTCTTGGAACTAGAGTCGGGGTTAGACCGTCGCCTGTGGTGGCAGCGTTTTCATCCAGAGGGCCTAATCTGGTAAGCCTTGAGATTCTCAAGCCGGATATGGTGGCACCAGGAGTGAACATCCTCGCCGCTTGGACAGGCGCATTAGGCCCGTCCAGCTTGCCAGTAGATCGTAGAAACGTGAAGTTCAATATACTGTCCGGGACTTCAATGTCCTGCCCCCATGTGAGCGGCATTGCCGCTCTGCTCAAGGCAAGGCACCCGGATTGGAGTCCGGCTGCAATAAAGTCCGCCTTGATGACAACCGCTTATGTCCACGACAACACGCGAAAGCCTCTACAAGACTCCTCAGCAGCTTCAATTTCAACCCCGTATGATCACGGTGCGGGGCACATAAACCCGGTTAGGGCTCTTGACCCGGGATTGATTTACGACATTGAGGCGCAGGACTATCTTGAATTCCTCTGCACACAAAGGCTAACACCAACGCAGCTCAAAGTTTTTACCAAGTACTCGAACAGAAGTTGCAAGAACAATCTAGCCAGTCCGGGGGACTTGAACTATCCGGCACTCTCGGTTGTGTTCCCGGACAGAACAAATGTTTCTGTTTTGACACTTCACAGAACTGTCACTAACGTTGGCCCTGCGGTTTCGAATTACCACGCCATTGTTTCACCGTTCAAAGGTGCTTATGTGAAAGTCGAGCCTCGGATGCTGAAATTCACCACAGCCAATCAGAAGCTGTCATACAAAATCATCTTCACTACGAAATCCCGGCAGGCAGTCCCGGAGTTCGGCGGGTTGGTGTGGAAGGATGGAGTGCACAGGGTGAGAAGCCCCATTGTTGTAGTGTGGCTGCCACCACTATAA

Protein Analysis

441

Amino Acids

47.21

Weight (kDa)

9.69

Isoelectric Point (pI)

40.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 46 - 134 1.1e-12 PA domain
Peptidase_S8 PF00082 88 - 260 1.5e-21 Subtilase family
fn3_6 PF17766 336 - 434 8e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 664
AccB1I GGYRCC 2 cut(s) 559, 711
AccBSI CCGCTC 2 cut(s) 684, 695
AccII CGCG 1 cut(s) 781
AclI AACGTT 1 cut(s) 1089
AclWI GGATC 1 cut(s) 219
AcoI YGGCCR 1 cut(s) 225
AcsI RAATTY 2 cut(s) 911, 1172
AdeI CACNNNGTG 1 cut(s) 1282
AfaI GTAC 2 cut(s) 16, 965
AfiI CCNNNNNNNGG 6 cut(s) 223, 370, 859, 1002, 1136, 1247
AgsI TTSAA 9 cut(s) 168, 423, 643, 663, 813, 819, 911, 1012, 1135
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 1 cut(s) 562
AluBI AGCT 9 cut(s) 29, 67, 186, 329, 346, 612, 809, 946, 1195
AluI AGCT 9 cut(s) 29, 67, 186, 329, 346, 612, 809, 946, 1195
Alw21I GWGCWC 2 cut(s) 188, 1278
Alw44I GTGCAC 1 cut(s) 1274
AlwI GGATC 1 cut(s) 219
AlwNI CAGNNNCTG 2 cut(s) 59, 1195
Ama87I CYCGRG 2 cut(s) 430, 871
AoxI GGCC 4 cut(s) 225, 515, 601, 1093
ApaLI GTGCAC 1 cut(s) 1274
ApeKI GCWGC 6 cut(s) 329, 497, 731, 806, 943, 1309
ApoI RAATTY 2 cut(s) 911, 1172
Asp700I GAANNNNTTC 1 cut(s) 163
AspLEI GCGC 2 cut(s) 596, 898
AspS9I GGNCC 3 cut(s) 515, 602, 1094
AsuHPI GGTGA 4 cut(s) 298, 1119, 1168, 1294
AsuII TTCGAA 1 cut(s) 1106
AsuNHI GCTAGC 1 cut(s) 29
AvaI CYCGRG 2 cut(s) 430, 871
BaeGI GKGCMC 2 cut(s) 846, 1278
BanI GGYRCC 2 cut(s) 559, 711
BanII GRGCYC 2 cut(s) 188, 865
BarI GAAGNNNNNNTAC 2 cut(s) 1184, 1216
Bbv12I GWGCWC 2 cut(s) 188, 1278
BbvCI CCTCAGC 1 cut(s) 802
BbvI GCAGC 6 cut(s) 316, 509, 718, 818, 955, 1296
BccI CCATC 1 cut(s) 1262
BciT130I CCWGG 1 cut(s) 564
BclI TGATCA 1 cut(s) 829
BcuI ACTAGT 1 cut(s) 170
BfaI CTAG 5 cut(s) 30, 171, 264, 465, 992
BfmI CTRYAG 1 cut(s) 103
BisI GCNGC 9 cut(s) 330, 498, 582, 685, 693, 732, 807, 944, 1310
BlsI GCNGC 9 cut(s) 331, 499, 583, 686, 694, 733, 808, 945, 1311
BmcAI AGTACT 2 cut(s) 16, 965
BmeT110I CYCGRG 2 cut(s) 430, 871
BmgT120I GGNCC 3 cut(s) 515, 602, 1094
BmiI GGNNCC 2 cut(s) 561, 713
BmsI GCATC 2 cut(s) 108, 1155
BmtI GCTAGC 1 cut(s) 33
Bpu10I CCTNAGC 1 cut(s) 802
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 3 cut(s) 527, 554, 686
BsaJI CCNNGG 6 cut(s) 36, 90, 250, 871, 1001, 1160
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 270, 300
Bsc4I CCNNNNNNNGG 6 cut(s) 223, 370, 859, 1002, 1136, 1247
Bse1I ACTGG 2 cut(s) 617, 996
Bse3DI GCAATG 1 cut(s) 687
BseBI CCWGG 1 cut(s) 564
BseDI CCNNGG 6 cut(s) 36, 90, 250, 871, 1001, 1160
BseGI GGATG 6 cut(s) 120, 315, 506, 573, 1170, 1273
BseLI CCNNNNNNNGG 6 cut(s) 223, 370, 859, 1002, 1136, 1247
BseMI GCAATG 1 cut(s) 687
BseMII CTCAG 1 cut(s) 816
BseNI ACTGG 2 cut(s) 617, 996
BseRI GAGGAG 3 cut(s) 315, 356, 790
BseSI GKGCMC 2 cut(s) 846, 1278
BseXI GCAGC 6 cut(s) 316, 509, 718, 818, 955, 1296
BsgI GTGCAG 2 cut(s) 290, 904
Bsh1236I CGCG 1 cut(s) 781
Bsh1285I CGRYCG 1 cut(s) 250
BshFI GGCC 4 cut(s) 227, 517, 603, 1095
BshNI GGYRCC 2 cut(s) 559, 711
BsiEI CGRYCG 1 cut(s) 250
BsiHKAI GWGCWC 2 cut(s) 188, 1278
BsiHKCI CYCGRG 2 cut(s) 430, 871
BslFI GGGAC 4 cut(s) 217, 670, 1019, 1223
BslI CCNNNNNNNGG 6 cut(s) 223, 370, 859, 1002, 1136, 1247
BsmFI GGGAC 4 cut(s) 217, 670, 1019, 1223
BsnI GGCC 4 cut(s) 227, 517, 603, 1095
BsoBI CYCGRG 2 cut(s) 430, 871
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 4 cut(s) 188, 846, 865, 1278
Bsp143I GATC 4 cut(s) 211, 312, 622, 829
Bsp19I CCATGG 1 cut(s) 36
BspANI GGCC 4 cut(s) 227, 517, 603, 1095
BspCNI CTCAG 1 cut(s) 815
BspFNI CGCG 1 cut(s) 781
BspLI GGNNCC 2 cut(s) 561, 713
BspOI GCTAGC 1 cut(s) 33
BspPI GGATC 1 cut(s) 219
BspT104I TTCGAA 1 cut(s) 1106
BspT107I GGYRCC 2 cut(s) 559, 711
BsrBI CCGCTC 2 cut(s) 684, 695
BsrDI GCAATG 1 cut(s) 687
BsrI ACTGG 2 cut(s) 617, 996
BssECI CCNNGG 6 cut(s) 36, 90, 250, 871, 1001, 1160
BssMI GATC 4 cut(s) 211, 312, 622, 829
BssT1I CCWWGG 1 cut(s) 36
Bst2UI CCWGG 1 cut(s) 564
Bst4CI ACNGT 8 cut(s) 22, 61, 251, 483, 651, 836, 1081, 1131
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 4 cut(s) 31, 188, 614, 1233
BstDEI CTNAG 1 cut(s) 802
BstDSI CCRYGG 2 cut(s) 36, 250
BstF5I GGATG 6 cut(s) 120, 315, 506, 573, 1170, 1273
BstFNI CGCG 1 cut(s) 781
BstHHI GCGC 2 cut(s) 596, 898
BstKTI GATC 4 cut(s) 214, 315, 625, 832
BstMBI GATC 4 cut(s) 211, 312, 622, 829
BstMCI CGRYCG 1 cut(s) 250
BstMWI GCNNNNNNNGC 4 cut(s) 326, 600, 609, 1165
BstNI CCWGG 1 cut(s) 564
BstSFI CTRYAG 1 cut(s) 103
BstSLI GKGCMC 2 cut(s) 846, 1278
BstUI CGCG 1 cut(s) 781
BstV1I GCAGC 6 cut(s) 316, 509, 718, 818, 955, 1296
BsuRI GGCC 4 cut(s) 227, 517, 603, 1095
BtgI CCRYGG 2 cut(s) 36, 250
BtsCI GGATG 6 cut(s) 120, 315, 506, 573, 1170, 1273
BtsIMutI CAGTG 1 cut(s) 57
Cac8I GCNNGC 4 cut(s) 31, 188, 614, 1233
CaiI CAGNNNCTG 2 cut(s) 59, 1195
CfoI GCGC 2 cut(s) 596, 898
Cfr13I GGNCC 3 cut(s) 515, 602, 1094
Cfr9I CCCGGG 1 cut(s) 871
Csp6I GTAC 2 cut(s) 15, 964
CviAII CATG 2 cut(s) 37, 677
CviQI GTAC 2 cut(s) 15, 964
DdeI CTNAG 1 cut(s) 802
DpnI GATC 4 cut(s) 213, 314, 624, 831
DpnII GATC 4 cut(s) 211, 312, 622, 829
DraIII CACNNNGTG 1 cut(s) 1282
DrdI GACNNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 664
EaeI YGGCCR 1 cut(s) 225
EciI GGCGGA 2 cut(s) 368, 733
Ecl136II GAGCTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 36
Eco24I GRGCYC 2 cut(s) 188, 865
Eco53kI GAGCTC 1 cut(s) 186
Eco88I CYCGRG 2 cut(s) 430, 871
EcoICRI GAGCTC 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 515
EcoRI GAATTC 1 cut(s) 911
EcoRII CCWGG 1 cut(s) 562
EcoT14I CCWWGG 1 cut(s) 36
EcoT38I GRGCYC 2 cut(s) 188, 865
ErhI CCWWGG 1 cut(s) 36
FaeI CATG 2 cut(s) 40, 680
FalI AAGNNNNNCTT 2 cut(s) 731, 763
FaqI GGGAC 4 cut(s) 217, 670, 1019, 1223
FatI CATG 2 cut(s) 36, 676
FauI CCCGC 2 cut(s) 832, 1244
FbaI TGATCA 1 cut(s) 829
Fnu4HI GCNGC 9 cut(s) 330, 498, 582, 685, 693, 732, 807, 944, 1310
FokI GGATG 6 cut(s) 127, 322, 493, 560, 1177, 1280
FriOI GRGCYC 2 cut(s) 188, 865
Fsp4HI GCNGC 9 cut(s) 330, 498, 582, 685, 693, 732, 807, 944, 1310
FspBI CTAG 5 cut(s) 30, 171, 264, 465, 992
GlaI GCGC 2 cut(s) 595, 897
GluI GCNGC 9 cut(s) 330, 498, 582, 685, 693, 732, 807, 944, 1310
HaeIII GGCC 4 cut(s) 227, 517, 603, 1095
HhaI GCGC 2 cut(s) 596, 898
Hin1II CATG 2 cut(s) 40, 680
Hin6I GCGC 2 cut(s) 594, 896
HinP1I GCGC 2 cut(s) 594, 896
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HinfI GANTC 5 cut(s) 405, 468, 538, 724, 797
HphI GGTGA 4 cut(s) 298, 1119, 1168, 1294
Hpy166II GTNNAC 4 cut(s) 126, 571, 769, 1276
Hpy188I TCNGA 4 cut(s) 71, 183, 1164, 1191
Hpy188III TCNNGA 3 cut(s) 510, 866, 908
Hpy8I GTNNAC 4 cut(s) 126, 571, 769, 1276
Hpy99I CGWCG 1 cut(s) 487
HpyAV CCTTC 3 cut(s) 105, 268, 1258
HpyCH4III ACNGT 8 cut(s) 22, 61, 251, 483, 651, 836, 1081, 1131
HpyCH4IV ACGT 3 cut(s) 206, 633, 1089
HpyCH4V TGCA 6 cut(s) 271, 332, 734, 921, 981, 1276
HpyF10VI GCNNNNNNNGC 4 cut(s) 326, 600, 609, 1165
HpyF3I CTNAG 1 cut(s) 802
HpySE526I ACGT 3 cut(s) 206, 633, 1089
Hsp92II CATG 2 cut(s) 40, 680
HspAI GCGC 2 cut(s) 594, 896
Ksp22I TGATCA 1 cut(s) 829
Kzo9I GATC 4 cut(s) 211, 312, 622, 829
LmnI GCTCC 2 cut(s) 26, 343
Lsp1109I GCAGC 6 cut(s) 316, 509, 718, 818, 955, 1296
LweI GCATC 2 cut(s) 108, 1155
MaeI CTAG 5 cut(s) 30, 171, 264, 465, 992
MaeII ACGT 3 cut(s) 206, 633, 1089
MaeIII GTNAC 1 cut(s) 1081
MalI GATC 4 cut(s) 213, 314, 624, 831
MbiI CCGCTC 2 cut(s) 684, 695
MboI GATC 4 cut(s) 211, 312, 622, 829
MboII GAAGA 1 cut(s) 1204
MhlI GDGCHC 4 cut(s) 188, 846, 865, 1278
MluCI AATT 5 cut(s) 163, 813, 911, 1108, 1172
MlyI GAGTC 3 cut(s) 477, 733, 791
MmeI TCCRAC 1 cut(s) 189
MroXI GAANNNNTTC 1 cut(s) 163
MspA1I CMGCKG 1 cut(s) 329
MvaI CCWGG 1 cut(s) 564
MvnI CGCG 1 cut(s) 781
MwoI GCNNNNNNNGC 4 cut(s) 326, 600, 609, 1165
NcoI CCATGG 1 cut(s) 36
NdeII GATC 4 cut(s) 211, 312, 622, 829
NheI GCTAGC 1 cut(s) 29
NlaIII CATG 2 cut(s) 40, 680
NlaIV GGNNCC 2 cut(s) 561, 713
NmeAIII GCCGAG 1 cut(s) 242
NmuCI GTSAC 1 cut(s) 1081
NspV TTCGAA 1 cut(s) 1106
PdmI GAANNNNTTC 1 cut(s) 163
PfeI GAWTC 2 cut(s) 405, 538
PfoI TCCNGGA 3 cut(s) 653, 1038, 1239
PkrI GCNGC 9 cut(s) 331, 499, 583, 686, 694, 733, 808, 945, 1311
PleI GAGTC 3 cut(s) 476, 732, 791
PpsI GAGTC 3 cut(s) 476, 732, 791
Psp124BI GAGCTC 1 cut(s) 188
Psp1406I AACGTT 1 cut(s) 1089
Psp6I CCWGG 1 cut(s) 562
PspGI CCWGG 1 cut(s) 562
PspN4I GGNNCC 2 cut(s) 561, 713
PspPI GGNCC 3 cut(s) 515, 602, 1094
PstNI CAGNNNCTG 2 cut(s) 59, 1195
PvuII CAGCTG 1 cut(s) 329
RsaI GTAC 2 cut(s) 16, 965
RsaNI GTAC 2 cut(s) 15, 964
SacI GAGCTC 1 cut(s) 188
SatI GCNGC 9 cut(s) 330, 498, 582, 685, 693, 732, 807, 944, 1310
Sau3AI GATC 4 cut(s) 211, 312, 622, 829
Sau96I GGNCC 3 cut(s) 515, 602, 1094
ScaI AGTACT 2 cut(s) 16, 965
SchI GAGTC 3 cut(s) 477, 733, 791
SduI GDGCHC 4 cut(s) 188, 846, 865, 1278
SfaNI GCATC 2 cut(s) 108, 1155
SfcI CTRYAG 1 cut(s) 103
SfuI TTCGAA 1 cut(s) 1106
SmaI CCCGGG 1 cut(s) 873
SmlI CTYRAG 3 cut(s) 533, 542, 701
SmoI CTYRAG 3 cut(s) 533, 542, 701
SpeI ACTAGT 1 cut(s) 170
Sse9I AATT 5 cut(s) 163, 813, 911, 1108, 1172
SspMI CTAG 5 cut(s) 30, 171, 264, 465, 992
SstI GAGCTC 1 cut(s) 188
StyI CCWWGG 1 cut(s) 36
TaaI ACNGT 8 cut(s) 22, 61, 251, 483, 651, 836, 1081, 1131
TaiI ACGT 3 cut(s) 209, 636, 1092
TaqI TCGA 3 cut(s) 968, 1106, 1155
TasI AATT 5 cut(s) 163, 813, 911, 1108, 1172
TatI WGTACW 2 cut(s) 14, 963
TauI GCSGC 3 cut(s) 584, 687, 695
TfiI GAWTC 2 cut(s) 405, 538
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 1 cut(s) 1081
TseI GCWGC 6 cut(s) 329, 497, 731, 806, 943, 1309
Tsp45I GTSAC 1 cut(s) 1081
TspDTI ATGAA 1 cut(s) 495
TspGWI ACGGA 1 cut(s) 351
TspMI CCCGGG 1 cut(s) 871
TspRI CASTG 1 cut(s) 64
VneI GTGCAC 1 cut(s) 1274
XapI RAATTY 2 cut(s) 911, 1172
XmaI CCCGGG 1 cut(s) 871
XmnI GAANNNNTTC 1 cut(s) 163
XspI CTAG 5 cut(s) 30, 171, 264, 465, 992
ZrmI AGTACT 2 cut(s) 16, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.