MD01G1190400.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013557
Physical Location & Seq
Forward (+)
28916720 .. 28917928
1209 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1190400.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGAAGCTGTCAACAAACAAGCAATACCCTGTTGTGTATTTGGGAAGCAATTCAACTATTCATAATCCGAGCTCGCTCAGTTTGGAGGGGACGTTGGATCGCCAAGTTGTGGCCGGAAAGATTGTGATTTGCGACCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGAAGTGGTGAAAAAAGCCGGAGGAGTTGGGATGATCTTGGCAAACATAGCTGCAAACGGAGAGGAGCTTGTGGCGGATTGCCACCTTGTTCCGGCGGTTGCTGTGGGAGAGAACGAAGCAAAGGGAATCAAGCATTATGCTTCAACAAGCCCGAGAGCCACCGCAACTCTAACATTTCTTGGAACTAGAGTTGGGGTTAGACCGTCGCCTGTGGTGGCAGCGTTTTCATCCAGAGGGCCTAATCTGGTAAGCCTTGATATTCTCAAGCCGGATATGGTGGCACCAGGAGTGAACATCATCGCCGCTTGGACAGGCGGATTGGGCCCGTCCAGCTTGCCAGCAGATCGTAGAAGGGTGAAGTTCAATATACTGTCCGGGACTTCAATGTCCTGCCCCCATGTGAGCGGCATTGCCGCTCTACTCAAGGCAAGGCACCCGGATTGGAGTCCGGCTGCAATAAAGTCCGCCTTGATGACAACCGCGTATGTCCACGACAACACGCGAAAGCCTCTACAAGACTCCTCAGCAGCTTCAATTTCAACCCCGTATGATCACGGTGCAGGGCACATAAACCCGGTTAGGGCTCTTGACCTGGGATTGATTTACGACATTGAGGCGCAGGACTATCTTGAATTCCTCTGCACACAAAGGCTAACACCAACCCAGCTCAAAGTTTTTACCAAGTACTCAAACAGAAGTTGCAAGAACAATCTAGCCAGTCCCGGGGAGTTGAACTATCCGGCACTTTCGGTTGTGTTCCCGGAGAGAACAAATGTTTCTGTTTTGACACTTCACAGAACTGTCACTAACGTTGGCCCTGCGGTTTCGAATTACCACGCCATTGTTTCACCGTTCAAAGGTGCTTATGTGAAAGTCGAGCCTCGGACGCTGAAATTCACCAAAGCCAATCAGAAGCTGTCATACAAAATCATCTTCACTACGAAATCCCGGCAGGCAGTCCCGGAGTTCGGAGGGTTGGTGTGGAAGGATGGAGTGCATAGGGTGAGAAGCCCCATTGTTGTAGTGTGGCTGCCACCACTATAA

Protein Analysis

403

Amino Acids

43.21

Weight (kDa)

9.78

Isoelectric Point (pI)

45.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PA PF02225 8 - 96 6.9e-12 PA domain
Peptidase_S8 PF00082 95 - 222 3.6e-21 Subtilase family
fn3_6 PF17766 298 - 396 3.6e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 550
AccB1I GGYRCC 2 cut(s) 445, 597
AccB7I CCANNNNNTGG 1 cut(s) 109
AccBSI CCGCTC 2 cut(s) 570, 581
AccII CGCG 2 cut(s) 647, 667
AclI AACGTT 1 cut(s) 975
AclWI GGATC 1 cut(s) 105
AcoI YGGCCR 1 cut(s) 111
AcsI RAATTY 2 cut(s) 797, 1058
AfaI GTAC 1 cut(s) 851
AfiI CCNNNNNNNGG 6 cut(s) 109, 256, 745, 888, 1022, 1133
AgsI TTSAA 9 cut(s) 54, 309, 529, 549, 699, 705, 797, 898, 1021
AjnI CCWGG 2 cut(s) 448, 756
AluBI AGCT 8 cut(s) 7, 72, 215, 232, 498, 695, 832, 1081
AluI AGCT 8 cut(s) 7, 72, 215, 232, 498, 695, 832, 1081
Alw21I GWGCWC 1 cut(s) 74
AlwI GGATC 1 cut(s) 105
AlwNI CAGNNNCTG 1 cut(s) 1081
Ama87I CYCGRG 2 cut(s) 316, 887
AoxI GGCC 4 cut(s) 111, 401, 487, 979
ApaI GGGCCC 1 cut(s) 491
ApeKI GCWGC 5 cut(s) 215, 383, 617, 692, 1195
ApoI RAATTY 2 cut(s) 797, 1058
Asp700I GAANNNNTTC 1 cut(s) 49
AspLEI GCGC 1 cut(s) 784
AspS9I GGNCC 4 cut(s) 401, 487, 488, 980
AsuC2I CCSGG 8 cut(s) 541, 602, 740, 888, 889, 926, 1114, 1127
AsuHPI GGTGA 5 cut(s) 184, 532, 1005, 1054, 1180
AsuII TTCGAA 1 cut(s) 992
AvaI CYCGRG 2 cut(s) 316, 887
BaeGI GKGCMC 2 cut(s) 491, 732
BanI GGYRCC 2 cut(s) 445, 597
BanII GRGCYC 3 cut(s) 74, 491, 751
BarI GAAGNNNNNNTAC 2 cut(s) 1070, 1102
Bbv12I GWGCWC 1 cut(s) 74
BbvCI CCTCAGC 1 cut(s) 688
BbvI GCAGC 5 cut(s) 202, 395, 604, 704, 1182
BccI CCATC 1 cut(s) 1148
BciT130I CCWGG 2 cut(s) 450, 758
BclI TGATCA 1 cut(s) 715
BcnI CCSGG 8 cut(s) 541, 602, 740, 888, 889, 926, 1114, 1127
BfaI CTAG 3 cut(s) 150, 351, 878
BisI GCNGC 8 cut(s) 216, 384, 468, 571, 579, 618, 693, 1196
BlsI GCNGC 8 cut(s) 217, 385, 469, 572, 580, 619, 694, 1197
BmcAI AGTACT 1 cut(s) 851
BmeT110I CYCGRG 2 cut(s) 316, 887
BmgT120I GGNCC 4 cut(s) 401, 487, 488, 980
BmiI GGNNCC 3 cut(s) 447, 489, 599
Bpu10I CCTNAGC 1 cut(s) 688
Bpu14I TTCGAA 1 cut(s) 992
BpuEI CTTGAG 2 cut(s) 413, 572
BpuMI CCSGG 8 cut(s) 541, 602, 740, 888, 889, 926, 1114, 1127
BsaJI CCNNGG 5 cut(s) 136, 757, 887, 888, 1046
BsaXI ACNNNNNCTCC 6 cut(s) 77, 107, 156, 186, 1128, 1158
Bsc4I CCNNNNNNNGG 6 cut(s) 109, 256, 745, 888, 1022, 1133
Bse1I ACTGG 1 cut(s) 882
Bse3DI GCAATG 1 cut(s) 573
BseBI CCWGG 2 cut(s) 450, 758
BseDI CCNNGG 5 cut(s) 136, 757, 887, 888, 1046
BseGI GGATG 3 cut(s) 201, 392, 1159
BseLI CCNNNNNNNGG 6 cut(s) 109, 256, 745, 888, 1022, 1133
BseMI GCAATG 1 cut(s) 573
BseMII CTCAG 2 cut(s) 91, 702
BseNI ACTGG 1 cut(s) 882
BseRI GAGGAG 3 cut(s) 201, 242, 676
BseSI GKGCMC 2 cut(s) 491, 732
BseXI GCAGC 5 cut(s) 202, 395, 604, 704, 1182
BseYI CCCAGC 1 cut(s) 828
BsgI GTGCAG 3 cut(s) 176, 744, 790
Bsh1236I CGCG 2 cut(s) 647, 667
Bsh1285I CGRYCG 1 cut(s) 136
BshFI GGCC 4 cut(s) 113, 403, 489, 981
BshNI GGYRCC 2 cut(s) 445, 597
BsiEI CGRYCG 1 cut(s) 136
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 2 cut(s) 316, 887
BslFI GGGAC 4 cut(s) 103, 556, 870, 1109
BslI CCNNNNNNNGG 6 cut(s) 109, 256, 745, 888, 1022, 1133
BsmFI GGGAC 4 cut(s) 103, 556, 870, 1109
BsnI GGCC 4 cut(s) 113, 403, 489, 981
BsoBI CYCGRG 2 cut(s) 316, 887
Bsp119I TTCGAA 1 cut(s) 992
Bsp120I GGGCCC 1 cut(s) 487
Bsp1286I GDGCHC 4 cut(s) 74, 491, 732, 751
Bsp143I GATC 4 cut(s) 97, 198, 508, 715
BspANI GGCC 4 cut(s) 113, 403, 489, 981
BspCNI CTCAG 2 cut(s) 90, 701
BspFNI CGCG 2 cut(s) 647, 667
BspLI GGNNCC 3 cut(s) 447, 489, 599
BspPI GGATC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 992
BspT107I GGYRCC 2 cut(s) 445, 597
BsrBI CCGCTC 2 cut(s) 570, 581
BsrDI GCAATG 1 cut(s) 573
BsrI ACTGG 1 cut(s) 882
BssECI CCNNGG 5 cut(s) 136, 757, 887, 888, 1046
BssMI GATC 4 cut(s) 97, 198, 508, 715
Bst2UI CCWGG 2 cut(s) 450, 758
Bst4CI ACNGT 6 cut(s) 137, 369, 537, 722, 967, 1017
BstBI TTCGAA 1 cut(s) 992
BstC8I GCNNGC 4 cut(s) 74, 500, 504, 1119
BstDEI CTNAG 2 cut(s) 77, 688
BstDSI CCRYGG 1 cut(s) 136
BstF5I GGATG 3 cut(s) 201, 392, 1159
BstFNI CGCG 2 cut(s) 647, 667
BstHHI GCGC 1 cut(s) 784
BstKTI GATC 4 cut(s) 100, 201, 511, 718
BstMBI GATC 4 cut(s) 97, 198, 508, 715
BstMCI CGRYCG 1 cut(s) 136
BstMWI GCNNNNNNNGC 4 cut(s) 212, 486, 495, 1051
BstNI CCWGG 2 cut(s) 450, 758
BstSLI GKGCMC 2 cut(s) 491, 732
BstUI CGCG 2 cut(s) 647, 667
BstV1I GCAGC 5 cut(s) 202, 395, 604, 704, 1182
BsuRI GGCC 4 cut(s) 113, 403, 489, 981
BtgI CCRYGG 1 cut(s) 136
BtgZI GCGATG 1 cut(s) 448
BtsCI GGATG 3 cut(s) 201, 392, 1159
Cac8I GCNNGC 4 cut(s) 74, 500, 504, 1119
CaiI CAGNNNCTG 1 cut(s) 1081
CfoI GCGC 1 cut(s) 784
Cfr13I GGNCC 4 cut(s) 401, 487, 488, 980
Cfr9I CCCGGG 1 cut(s) 887
CseI GACGC 1 cut(s) 1060
Csp6I GTAC 1 cut(s) 850
CviAII CATG 1 cut(s) 563
CviQI GTAC 1 cut(s) 850
DdeI CTNAG 2 cut(s) 77, 688
DpnI GATC 4 cut(s) 99, 200, 510, 717
DpnII GATC 4 cut(s) 97, 198, 508, 715
DrdI GACNNNNNNGTC 1 cut(s) 550
DseDI GACNNNNNNGTC 1 cut(s) 550
EaeI YGGCCR 1 cut(s) 111
EciI GGCGGA 3 cut(s) 254, 495, 619
Ecl136II GAGCTC 1 cut(s) 72
Eco24I GRGCYC 3 cut(s) 74, 491, 751
Eco53kI GAGCTC 1 cut(s) 72
Eco88I CYCGRG 2 cut(s) 316, 887
EcoICRI GAGCTC 1 cut(s) 72
EcoO109I RGGNCCY 1 cut(s) 401
EcoRI GAATTC 1 cut(s) 797
EcoRII CCWGG 2 cut(s) 448, 756
EcoT38I GRGCYC 3 cut(s) 74, 491, 751
FaeI CATG 1 cut(s) 566
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 4 cut(s) 103, 556, 870, 1109
FatI CATG 1 cut(s) 562
FbaI TGATCA 1 cut(s) 715
Fnu4HI GCNGC 8 cut(s) 216, 384, 468, 571, 579, 618, 693, 1196
FokI GGATG 3 cut(s) 208, 379, 1166
FriOI GRGCYC 3 cut(s) 74, 491, 751
Fsp4HI GCNGC 8 cut(s) 216, 384, 468, 571, 579, 618, 693, 1196
FspBI CTAG 3 cut(s) 150, 351, 878
GlaI GCGC 1 cut(s) 783
GluI GCNGC 8 cut(s) 216, 384, 468, 571, 579, 618, 693, 1196
GsaI CCCAGC 1 cut(s) 832
HaeIII GGCC 4 cut(s) 113, 403, 489, 981
HgaI GACGC 1 cut(s) 1060
HhaI GCGC 1 cut(s) 784
Hin1II CATG 1 cut(s) 566
Hin6I GCGC 1 cut(s) 782
HinP1I GCGC 1 cut(s) 782
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 3 cut(s) 291, 610, 683
HphI GGTGA 5 cut(s) 184, 532, 1005, 1054, 1180
Hpy166II GTNNAC 3 cut(s) 12, 457, 655
Hpy188I TCNGA 4 cut(s) 69, 1050, 1077, 1136
Hpy188III TCNNGA 3 cut(s) 396, 752, 794
Hpy8I GTNNAC 3 cut(s) 12, 457, 655
Hpy99I CGWCG 1 cut(s) 373
HpyAV CCTTC 3 cut(s) 154, 510, 1144
HpyCH4III ACNGT 6 cut(s) 137, 369, 537, 722, 967, 1017
HpyCH4IV ACGT 2 cut(s) 92, 975
HpyCH4V TGCA 7 cut(s) 157, 218, 620, 725, 807, 867, 1162
HpyF10VI GCNNNNNNNGC 4 cut(s) 212, 486, 495, 1051
HpyF3I CTNAG 2 cut(s) 77, 688
HpySE526I ACGT 2 cut(s) 92, 975
Hsp92II CATG 1 cut(s) 566
HspAI GCGC 1 cut(s) 782
Ksp22I TGATCA 1 cut(s) 715
Kzo9I GATC 4 cut(s) 97, 198, 508, 715
LmnI GCTCC 1 cut(s) 229
Lsp1109I GCAGC 5 cut(s) 202, 395, 604, 704, 1182
MaeI CTAG 3 cut(s) 150, 351, 878
MaeII ACGT 2 cut(s) 92, 975
MaeIII GTNAC 1 cut(s) 967
MalI GATC 4 cut(s) 99, 200, 510, 717
MbiI CCGCTC 2 cut(s) 570, 581
MboI GATC 4 cut(s) 97, 198, 508, 715
MboII GAAGA 1 cut(s) 1090
MhlI GDGCHC 4 cut(s) 74, 491, 732, 751
MluCI AATT 5 cut(s) 49, 699, 797, 994, 1058
MlyI GAGTC 2 cut(s) 619, 677
MmeI TCCRAC 1 cut(s) 75
MroXI GAANNNNTTC 1 cut(s) 49
MvaI CCWGG 2 cut(s) 450, 758
MvnI CGCG 2 cut(s) 647, 667
MwoI GCNNNNNNNGC 4 cut(s) 212, 486, 495, 1051
NciI CCSGG 8 cut(s) 541, 602, 740, 888, 889, 926, 1114, 1127
NdeII GATC 4 cut(s) 97, 198, 508, 715
NlaIII CATG 1 cut(s) 566
NlaIV GGNNCC 3 cut(s) 447, 489, 599
NmuCI GTSAC 1 cut(s) 967
NspV TTCGAA 1 cut(s) 992
PdmI GAANNNNTTC 1 cut(s) 49
PfeI GAWTC 1 cut(s) 291
PflMI CCANNNNNTGG 1 cut(s) 109
PfoI TCCNGGA 3 cut(s) 539, 924, 1125
PkrI GCNGC 8 cut(s) 217, 385, 469, 572, 580, 619, 694, 1197
PleI GAGTC 2 cut(s) 618, 677
PpsI GAGTC 2 cut(s) 618, 677
Psp124BI GAGCTC 1 cut(s) 74
Psp1406I AACGTT 1 cut(s) 975
Psp6I CCWGG 2 cut(s) 448, 756
PspFI CCCAGC 1 cut(s) 828
PspGI CCWGG 2 cut(s) 448, 756
PspN4I GGNNCC 3 cut(s) 447, 489, 599
PspOMI GGGCCC 1 cut(s) 487
PspPI GGNCC 4 cut(s) 401, 487, 488, 980
PstNI CAGNNNCTG 1 cut(s) 1081
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
SacI GAGCTC 1 cut(s) 74
SatI GCNGC 8 cut(s) 216, 384, 468, 571, 579, 618, 693, 1196
Sau3AI GATC 4 cut(s) 97, 198, 508, 715
Sau96I GGNCC 4 cut(s) 401, 487, 488, 980
ScaI AGTACT 1 cut(s) 851
SchI GAGTC 2 cut(s) 619, 677
SduI GDGCHC 4 cut(s) 74, 491, 732, 751
SfuI TTCGAA 1 cut(s) 992
SmaI CCCGGG 1 cut(s) 889
SmlI CTYRAG 2 cut(s) 428, 587
SmoI CTYRAG 2 cut(s) 428, 587
Sse9I AATT 5 cut(s) 49, 699, 797, 994, 1058
SspMI CTAG 3 cut(s) 150, 351, 878
SstI GAGCTC 1 cut(s) 74
TaaI ACNGT 6 cut(s) 137, 369, 537, 722, 967, 1017
TaiI ACGT 2 cut(s) 95, 978
TaqI TCGA 2 cut(s) 992, 1041
TasI AATT 5 cut(s) 49, 699, 797, 994, 1058
TatI WGTACW 1 cut(s) 849
TauI GCSGC 3 cut(s) 470, 573, 581
TfiI GAWTC 1 cut(s) 291
TseFI GTSAC 1 cut(s) 967
TseI GCWGC 5 cut(s) 215, 383, 617, 692, 1195
Tsp45I GTSAC 1 cut(s) 967
TspDTI ATGAA 3 cut(s) 17, 50, 381
TspGWI ACGGA 1 cut(s) 237
TspMI CCCGGG 1 cut(s) 887
Van91I CCANNNNNTGG 1 cut(s) 109
XapI RAATTY 2 cut(s) 797, 1058
XmaI CCCGGG 1 cut(s) 887
XmnI GAANNNNTTC 1 cut(s) 49
XspI CTAG 3 cut(s) 150, 351, 878
ZrmI AGTACT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.