MD01G1188800.v1.1

subtilisin-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0013557
Physical Location & Seq
Forward (+)
28716918 .. 28719269
2352 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1188800.v1.1.491

Sequence Viewer

Length: 2352 bp
ATGAAAATGGTGGAAAAACCAGAGAAATGGCAGGTTTTAACCCTAACAATCTTCATGTTCTTCAACATTGCCCTCTCTGCGAAAACCCCATTGACAAAAAAGACCTACATTGTCCAGATGGACAAGTCAGCCAAGCCAGAGTCTTTCTCCAACCACATAGATTGGTACTCATCAAAAGTGCAATCCGTACTCATCAATCCAGAGAATCAAGAAGACGGAGGCGAAGATCAACAGAGGGTAATCTACACTTACCAGAACGCCTTCCACGGCGTCGCTGCCCGGCTAAGCGAGCAAGAAGCCGAGCGGCTAGAGGAGCAAGACGGAGTCTTGGCGATTTTCCCAGAGACTAAGTACGAGCTCCACACCACAAGGAGTCCCCTGTTTCTTGGGCTCGAACCGCATGTCAGAGCCAGCACCACCAATGTCTGGTCACAACGAGTCACCGACCACGACGTCATTGTCGGGGTGCTGGACACGGGTGTCTGGCCGGAGAGCCAGAGCTTCAACGACACTGGCATGACCCCAGTGCCGGCCCACTGGAAGGGAGCGTGCGAAACCGGCCGTAGCTTCACCAAAAGCAACTGCAACAAAAAAATTGTTGGAGCGAGAATTTTTTATCACGGATATGAGGCAGCCACGGGGAAGATCAACGAGCAGACGGAGTTTAAGTCGCCGAGAGATCAGGACGGGCATGGGACCCACACTGCCGCCACAGTTGCCGGGTCTCCGGTGCGCGGTGCTAATCTTCTCGGGTATGCTCGTGGAACGGCCAGAGGAATGGCGCCGAGTGCGAGAATTGCGGCGTACAAGGTTTGCTGGGTCGGCGGCTGCTTCAGCTCAGATATTCTGTCGGCGGTGGACAGAGCGGTGGCCGATGGAGTCAATGTGTTGTCTATATCTTTAGGAGGAGGTGTGTCTTCTTATTACCGGGACAGTTTGTCCGTGGCGGCTTTTGGGGCTATGGAGATGGGAATTTTCGTGTCCTGCTCCGCCGGAAATGGAGGGCCGGATCCTGTTAGTCTGACCAATGTGTCCCCCTGGATAACCACGGTTGGAGCTAGCACCATGGATAGGGATTTTCCAGCTACTGTGAAGCTCGGAAATGGCAGGACTATTACCGGGGTTTCGCTCTACAGGGGAAGGATGAAGCTGTCAACAAACAAGCAATACCCTGTTGTGTATTTGGGAAGCAATTCAACTAGTCCTAATCCGAGCTCGCTGTGTTTGGAGGGGACGTTGGATCGCCGAGTTGTGGCCGGAAAGATTGTGATTTGCGACCGTGGGATTAGTCCTAGAGTGCAGAAGGGAGAAGTGGTGAAAGAAGCCGGAGGAGTTGGGATGATCTTGGCAAACACAGCTGCAAACGGAGAGGAGCTTGTGGCGGATTGCCACCTTGTTCCGGCGGTTGCTGTGGGAGAGAACGAAGCAAAGGGAATCAAGCATTATGCTTCAACAAGCCCGAGAGCCACCGCAACTCTAACATTTCTTGGAACTAGAGTTGGGGTTAGACCGTCGCCTGTGGTGGCAGCGTTTTCATCCAGAGGGCCTAATCTGGTAAGCCTTGAGATTCTCAAGCCGGATATGGTGGCACCAGGAGTTAACATCCTCGCCGCTTGGACAGGCGCATTAGGCCCGTCCAGCTTGCCAGCAGATCGTAGAAACGTGAAGTTCAATATACTGTCCGGGACTTCAATGTCCTGCCCCCATGTGAGCGGCATTGCCGCTCTGCTCAAGGCAAGGCACCCGGATTGGAGTCCGGCTGCAATAAAGTCCGCCTTGATGACAACCGCGTATGTCCACGACAACACGCGAAAGCCTCTACAAGACTCCTCAGCAGCTTCAATTTCAACCCCGTATGATCACGGTGCAGGGCACATAAACCCGGTTAGGGCTCTTGACCCGGGATTGATTTACGACATTGAGGCGCAGGACTATCTTGAATTTCTCTGCACACAAAGGCTAACACCAACACAGCTCAAAGTTTTTACCAAGTACTCGAACAGAAGTTGCAAGAACAATCTAGCCAGTCCCGGGGACTTGAACTACCCGGCACTTTCGGTTGTGTTCCCGGAGAGAACAAATGTTTCTGTTTTGACACTTCACAGAACTGTCACTAACGTTGGCCCTGCGGTTTCGAATTACCACGCCATTGTTTCACCGTTCAAAGGTGCTTATGTGAAAGTCGAGCCTCGGACGCTGAAATTCACCAAAGCCAATCAGAAGCTGTCATACAAAATCATCTTCACTACGAAATCCCGGCAGGCAGTCCCGGAGTTCGGCGGGTTGGTGTGGAAGGATGGAGTGCACAGGGTGAGAAGCCCCATTGTTGTAGTGTGGCTGCCACCACTATAA

Protein Analysis

784

Amino Acids

84.48

Weight (kDa)

9.17

Isoelectric Point (pI)

38.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Inhibitor_I9 PF05922 35 - 121 1.6e-21 Peptidase inhibitor I9
Peptidase_S8 PF00082 150 - 603 5.5e-49 Subtilase family
PA PF02225 389 - 477 2.6e-12 PA domain
fn3_6 PF17766 679 - 777 4.3e-27 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013557)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51750
fragaria_vesca FvH4_7g28501
malus_domestica MD01G1188800.v1.1 MD01G1189100.v1.1 MD01G1189900.v1.1 MD01G1190400.v1.1
prunus_persica Prupe.2G285500_v2.0.a1
pyrus_communis pycom01g20050
rosa_chinensis RchiOBHm_Chr1g0375891
rosa_laevigata RLG00000026646
rosa_multiflora Rmu_sc0001943.1_g000010
rosa_roxburghii Rroxscaffold_4G00282550
rosa_rugosa Rorug01G0392200
rosa_samantha Rh1AG404100 Rh1BG367500 Rh1CG380300 Rh1DG397500
rosa_wichuraiana Rw0G000960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 5 cut(s) 452, 458, 479, 1030, 1693
AatII GACGTC 1 cut(s) 456
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 3 cut(s) 781, 1588, 1740
AccB7I CCANNNNNTGG 1 cut(s) 426
AccBSI CCGCTC 4 cut(s) 304, 866, 1713, 1724
AccII CGCG 3 cut(s) 735, 1790, 1810
AclI AACGTT 1 cut(s) 2118
AclWI GGATC 3 cut(s) 1004, 1017, 1248
AcoI YGGCCR 5 cut(s) 485, 559, 768, 870, 1254
AcsI RAATTY 4 cut(s) 609, 972, 1940, 2201
AcuI CTGAAG 1 cut(s) 817
AcyI GRCGYC 3 cut(s) 270, 453, 782
AdeI CACNNNGTG 1 cut(s) 2311
AfaI GTAC 5 cut(s) 167, 189, 353, 806, 1994
AhdI GACNNNNNGTC 1 cut(s) 937
AhlI ACTAGT 1 cut(s) 1199
AjnI CCWGG 2 cut(s) 1037, 1591
Alw21I GWGCWC 3 cut(s) 360, 1217, 2307
Alw26I GTCTC 2 cut(s) 338, 729
Alw44I GTGCAC 1 cut(s) 2303
AlwI GGATC 3 cut(s) 1004, 1017, 1248
AlwNI CAGNNNCTG 2 cut(s) 1088, 2224
Ama87I CYCGRG 4 cut(s) 749, 1459, 1900, 2030
ApaLI GTGCAC 1 cut(s) 2303
ApeKI GCWGC 8 cut(s) 275, 632, 828, 1358, 1526, 1760, 1835, 2338
ApoI RAATTY 4 cut(s) 609, 972, 1940, 2201
ArsI GACNNNNNNTTYG 2 cut(s) 387, 419
Asp700I GAANNNNTTC 2 cut(s) 260, 1192
AspLEI GCGC 4 cut(s) 735, 784, 1625, 1927
AspS9I GGNCC 6 cut(s) 532, 696, 1004, 1544, 1631, 2123
AsuHPI GGTGA 6 cut(s) 433, 562, 1327, 2148, 2197, 2323
AsuII TTCGAA 1 cut(s) 2135
AsuNHI GCTAGC 1 cut(s) 1058
AvaI CYCGRG 4 cut(s) 749, 1459, 1900, 2030
AvaII GGWCC 1 cut(s) 696
BaeGI GKGCMC 2 cut(s) 1875, 2307
BamHI GGATCC 1 cut(s) 1009
BanI GGYRCC 3 cut(s) 781, 1588, 1740
BanII GRGCYC 4 cut(s) 360, 393, 1217, 1894
BarI GAAGNNNNNNTAC 2 cut(s) 2213, 2245
BauI CACGAG 1 cut(s) 759
BbsI GAAGAC 2 cut(s) 219, 909
Bbv12I GWGCWC 3 cut(s) 360, 1217, 2307
BbvCI CCTCAGC 1 cut(s) 1831
BbvI GCAGC 8 cut(s) 262, 644, 815, 1345, 1538, 1747, 1847, 2325
BccI CCATC 4 cut(s) 112, 869, 961, 2291
BceAI ACGGC 3 cut(s) 283, 546, 783
BciT130I CCWGG 2 cut(s) 1039, 1593
BclI TGATCA 1 cut(s) 1858
BcoDI GTCTC 2 cut(s) 338, 729
BcuI ACTAGT 1 cut(s) 1199
BfaI CTAG 6 cut(s) 308, 1059, 1200, 1293, 1494, 2021
BfmI CTRYAG 1 cut(s) 1132
BfoI RGCGCY 1 cut(s) 785
BfuAI ACCTGC 1 cut(s) 22
BlpI GCTNAGC 1 cut(s) 284
BmcAI AGTACT 1 cut(s) 1994
Bme18I GGWCC 1 cut(s) 696
BmeRI GACNNNNNGTC 1 cut(s) 937
BmeT110I CYCGRG 4 cut(s) 749, 1459, 1900, 2030
BmgT120I GGNCC 6 cut(s) 532, 696, 1004, 1544, 1631, 2123
BmiI GGNNCC 6 cut(s) 697, 698, 783, 1011, 1590, 1742
BmrI ACTGGG 1 cut(s) 518
BmtI GCTAGC 1 cut(s) 1062
BmuI ACTGGG 1 cut(s) 518
BpiI GAAGAC 2 cut(s) 219, 909
BplI GAGNNNNNCTC 2 cut(s) 131, 163
Bpu10I CCTNAGC 1 cut(s) 1831
Bpu1102I GCTNAGC 1 cut(s) 284
Bpu14I TTCGAA 1 cut(s) 2135
BpuEI CTTGAG 3 cut(s) 1556, 1583, 1715
BsaHI GRCGYC 3 cut(s) 270, 453, 782
BsaI GGTCTC 1 cut(s) 729
BsaWI WCCGGW 1 cut(s) 727
Bse118I RCCGGY 2 cut(s) 529, 557
Bse1I ACTGG 4 cut(s) 517, 524, 542, 2025
Bse3DI GCAATG 2 cut(s) 66, 1716
BseBI CCWGG 2 cut(s) 1039, 1593
BseGI GGATG 5 cut(s) 1149, 1344, 1535, 1602, 2302
BseMI GCAATG 2 cut(s) 66, 1716
BseMII CTCAG 2 cut(s) 852, 1845
BseNI ACTGG 4 cut(s) 517, 524, 542, 2025
BseRI GAGGAG 5 cut(s) 326, 921, 1344, 1385, 1819
BseSI GKGCMC 2 cut(s) 1875, 2307
BseX3I CGGCCG 1 cut(s) 559
BseXI GCAGC 8 cut(s) 262, 644, 815, 1345, 1538, 1747, 1847, 2325
BseYI CCCAGC 1 cut(s) 816
BsgI GTGCAG 3 cut(s) 1319, 1887, 1933
Bsh1236I CGCG 3 cut(s) 735, 1790, 1810
Bsh1285I CGRYCG 2 cut(s) 562, 1279
BshNI GGYRCC 3 cut(s) 781, 1588, 1740
BsiEI CGRYCG 2 cut(s) 562, 1279
BsiHKAI GWGCWC 3 cut(s) 360, 1217, 2307
BsiHKCI CYCGRG 4 cut(s) 749, 1459, 1900, 2030
BslFI GGGAC 9 cut(s) 360, 709, 944, 1018, 1246, 1699, 2013, 2048, 2252
BsmAI GTCTC 2 cut(s) 338, 729
BsmFI GGGAC 9 cut(s) 360, 709, 944, 1018, 1246, 1699, 2013, 2048, 2252
Bso31I GGTCTC 1 cut(s) 729
BsoBI CYCGRG 4 cut(s) 749, 1459, 1900, 2030
Bsp119I TTCGAA 1 cut(s) 2135
Bsp1286I GDGCHC 6 cut(s) 360, 393, 1217, 1875, 1894, 2307
Bsp143I GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
Bsp1720I GCTNAGC 1 cut(s) 284
Bsp19I CCATGG 1 cut(s) 1065
BspCNI CTCAG 2 cut(s) 851, 1844
BspFNI CGCG 3 cut(s) 735, 1790, 1810
BspLI GGNNCC 6 cut(s) 697, 698, 783, 1011, 1590, 1742
BspMI ACCTGC 1 cut(s) 22
BspOI GCTAGC 1 cut(s) 1062
BspPI GGATC 3 cut(s) 1004, 1017, 1248
BspT104I TTCGAA 1 cut(s) 2135
BspT107I GGYRCC 3 cut(s) 781, 1588, 1740
BspTNI GGTCTC 1 cut(s) 729
BsrBI CCGCTC 4 cut(s) 304, 866, 1713, 1724
BsrDI GCAATG 2 cut(s) 66, 1716
BsrFI RCCGGY 2 cut(s) 529, 557
BsrI ACTGG 4 cut(s) 517, 524, 542, 2025
BssAI RCCGGY 2 cut(s) 529, 557
BssMI GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
BssNI GRCGYC 3 cut(s) 270, 453, 782
BssSI CACGAG 1 cut(s) 759
BssT1I CCWWGG 1 cut(s) 1065
Bst2BI CACGAG 1 cut(s) 759
Bst2UI CCWGG 2 cut(s) 1039, 1593
BstACI GRCGYC 3 cut(s) 270, 453, 782
BstBI TTCGAA 1 cut(s) 2135
BstC8I GCNNGC 9 cut(s) 290, 412, 531, 550, 1060, 1217, 1643, 1647, 2262
BstDEI CTNAG 4 cut(s) 284, 348, 838, 1831
BstDSI CCRYGG 6 cut(s) 265, 636, 942, 1047, 1065, 1279
BstF5I GGATG 5 cut(s) 1149, 1344, 1535, 1602, 2302
BstFNI CGCG 3 cut(s) 735, 1790, 1810
BstH2I RGCGCY 1 cut(s) 785
BstHHI GCGC 4 cut(s) 735, 784, 1625, 1927
BstKTI GATC 8 cut(s) 229, 648, 682, 1012, 1243, 1344, 1654, 1861
BstMAI GTCTC 2 cut(s) 338, 729
BstMBI GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
BstMCI CGRYCG 2 cut(s) 562, 1279
BstNI CCWGG 2 cut(s) 1039, 1593
BstNSI RCATGY 1 cut(s) 404
BstSFI CTRYAG 1 cut(s) 1132
BstSLI GKGCMC 2 cut(s) 1875, 2307
BstUI CGCG 3 cut(s) 735, 1790, 1810
BstV1I GCAGC 8 cut(s) 262, 644, 815, 1345, 1538, 1747, 1847, 2325
BstV2I GAAGAC 2 cut(s) 219, 909
BstX2I RGATCY 1 cut(s) 1009
BstXI CCANNNNNNTGG 2 cut(s) 27, 778
BstYI RGATCY 1 cut(s) 1009
BstZI CGGCCG 1 cut(s) 559
BtgI CCRYGG 6 cut(s) 265, 636, 942, 1047, 1065, 1279
BtsCI GGATG 5 cut(s) 1149, 1344, 1535, 1602, 2302
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 4 cut(s) 510, 531, 535, 702
BveI ACCTGC 1 cut(s) 22
Cac8I GCNNGC 9 cut(s) 290, 412, 531, 550, 1060, 1217, 1643, 1647, 2262
CaiI CAGNNNCTG 2 cut(s) 1088, 2224
CfoI GCGC 4 cut(s) 735, 784, 1625, 1927
Cfr10I RCCGGY 2 cut(s) 529, 557
Cfr13I GGNCC 6 cut(s) 532, 696, 1004, 1544, 1631, 2123
Cfr9I CCCGGG 2 cut(s) 1900, 2030
CseI GACGC 2 cut(s) 259, 2203
Csp6I GTAC 5 cut(s) 166, 188, 352, 805, 1993
CspCI CAANNNNNGTGG 2 cut(s) 143, 178
CviAII CATG 6 cut(s) 55, 401, 517, 692, 1066, 1706
CviQI GTAC 5 cut(s) 166, 188, 352, 805, 1993
DdeI CTNAG 4 cut(s) 284, 348, 838, 1831
DinI GGCGCC 1 cut(s) 783
DpnI GATC 8 cut(s) 228, 647, 681, 1011, 1242, 1343, 1653, 1860
DpnII GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
DraIII CACNNNGTG 1 cut(s) 2311
DrdI GACNNNNNNGTC 5 cut(s) 452, 458, 479, 1030, 1693
DriI GACNNNNNGTC 1 cut(s) 937
DseDI GACNNNNNNGTC 5 cut(s) 452, 458, 479, 1030, 1693
EaeI YGGCCR 5 cut(s) 485, 559, 768, 870, 1254
EagI CGGCCG 1 cut(s) 559
Eam1105I GACNNNNNGTC 1 cut(s) 937
EciI GGCGGA 3 cut(s) 979, 1397, 1762
Ecl136II GAGCTC 2 cut(s) 358, 1215
EclXI CGGCCG 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 1065
Eco24I GRGCYC 4 cut(s) 360, 393, 1217, 1894
Eco31I GGTCTC 1 cut(s) 729
Eco47I GGWCC 1 cut(s) 696
Eco52I CGGCCG 1 cut(s) 559
Eco53kI GAGCTC 2 cut(s) 358, 1215
Eco57I CTGAAG 1 cut(s) 817
Eco88I CYCGRG 4 cut(s) 749, 1459, 1900, 2030
EcoICRI GAGCTC 2 cut(s) 358, 1215
EcoO109I RGGNCCY 2 cut(s) 696, 1544
EcoRII CCWGG 2 cut(s) 1037, 1591
EcoT14I CCWWGG 1 cut(s) 1065
EcoT38I GRGCYC 4 cut(s) 360, 393, 1217, 1894
EgeI GGCGCC 1 cut(s) 783
EheI GGCGCC 1 cut(s) 783
ErhI CCWWGG 1 cut(s) 1065
FaeI CATG 6 cut(s) 58, 404, 520, 695, 1069, 1709
FalI AAGNNNNNCTT 2 cut(s) 1760, 1792
FaqI GGGAC 9 cut(s) 360, 709, 944, 1018, 1246, 1699, 2013, 2048, 2252
FatI CATG 6 cut(s) 54, 400, 516, 691, 1065, 1705
FauI CCCGC 1 cut(s) 2273
FbaI TGATCA 1 cut(s) 1858
FokI GGATG 5 cut(s) 1156, 1351, 1522, 1589, 2309
FriOI GRGCYC 4 cut(s) 360, 393, 1217, 1894
FspBI CTAG 6 cut(s) 308, 1059, 1200, 1293, 1494, 2021
GlaI GCGC 4 cut(s) 734, 783, 1624, 1926
GsaI CCCAGC 1 cut(s) 820
HaeII RGCGCY 1 cut(s) 785
HgaI GACGC 2 cut(s) 259, 2203
HhaI GCGC 4 cut(s) 735, 784, 1625, 1927
Hin1I GRCGYC 3 cut(s) 270, 453, 782
Hin1II CATG 6 cut(s) 58, 404, 520, 695, 1069, 1709
Hin6I GCGC 4 cut(s) 733, 782, 1623, 1925
HinP1I GCGC 4 cut(s) 733, 782, 1623, 1925
HincII GTYRAC 2 cut(s) 1155, 1600
HindII GTYRAC 2 cut(s) 1155, 1600
HpaI GTTAAC 1 cut(s) 1600
HphI GGTGA 6 cut(s) 433, 562, 1327, 2148, 2197, 2323
Hpy166II GTNNAC 5 cut(s) 859, 1155, 1600, 1798, 2305
Hpy188I TCNGA 7 cut(s) 407, 841, 1023, 1100, 1212, 2193, 2220
Hpy188III TCNNGA 7 cut(s) 115, 200, 209, 683, 1539, 1895, 1937
Hpy8I GTNNAC 5 cut(s) 859, 1155, 1600, 1798, 2305
Hpy99I CGWCG 3 cut(s) 275, 455, 1516
HpyAV CCTTC 5 cut(s) 271, 535, 1134, 1297, 2287
HpyCH4IV ACGT 4 cut(s) 453, 1235, 1662, 2118
HpyCH4V TGCA 9 cut(s) 181, 585, 1300, 1361, 1763, 1868, 1950, 2010, 2305
HpyF3I CTNAG 4 cut(s) 284, 348, 838, 1831
HpySE526I ACGT 4 cut(s) 453, 1235, 1662, 2118
Hsp92I GRCGYC 3 cut(s) 270, 453, 782
Hsp92II CATG 6 cut(s) 58, 404, 520, 695, 1069, 1709
HspAI GCGC 4 cut(s) 733, 782, 1623, 1925
KasI GGCGCC 1 cut(s) 781
KflI GGGWCCC 1 cut(s) 696
KroI GCCGGC 1 cut(s) 529
KroNI GCCGGC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 1858
KspAI GTTAAC 1 cut(s) 1600
Kzo9I GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
LmnI GCTCC 7 cut(s) 313, 363, 545, 602, 992, 1055, 1372
Lsp1109I GCAGC 8 cut(s) 262, 644, 815, 1345, 1538, 1747, 1847, 2325
MaeI CTAG 6 cut(s) 308, 1059, 1200, 1293, 1494, 2021
MaeII ACGT 4 cut(s) 453, 1235, 1662, 2118
MaeIII GTNAC 3 cut(s) 429, 439, 2110
MalI GATC 8 cut(s) 228, 647, 681, 1011, 1242, 1343, 1653, 1860
MbiI CCGCTC 4 cut(s) 304, 866, 1713, 1724
MboI GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
MboII GAAGA 8 cut(s) 43, 52, 224, 236, 655, 737, 909, 2233
MflI RGATCY 1 cut(s) 1009
MhlI GDGCHC 6 cut(s) 360, 393, 1217, 1875, 1894, 2307
MluCI AATT 9 cut(s) 594, 609, 795, 972, 1192, 1842, 1940, 2137, 2201
Mly113I GGCGCC 1 cut(s) 782
MlyI GAGTC 7 cut(s) 149, 333, 382, 447, 888, 1762, 1820
MmeI TCCRAC 4 cut(s) 174, 580, 1033, 1218
MroNI GCCGGC 1 cut(s) 529
MroXI GAANNNNTTC 2 cut(s) 260, 1192
MseI TTAA 3 cut(s) 38, 666, 1599
MslI CAYNNNNRTG 2 cut(s) 515, 624
MspA1I CMGCKG 1 cut(s) 1358
MvaI CCWGG 2 cut(s) 1039, 1593
MvnI CGCG 3 cut(s) 735, 1790, 1810
NaeI GCCGGC 1 cut(s) 531
NarI GGCGCC 1 cut(s) 782
NcoI CCATGG 1 cut(s) 1065
NdeII GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
NgoMIV GCCGGC 1 cut(s) 529
NheI GCTAGC 1 cut(s) 1058
NlaIII CATG 6 cut(s) 58, 404, 520, 695, 1069, 1709
NlaIV GGNNCC 6 cut(s) 697, 698, 783, 1011, 1590, 1742
NmeAIII GCCGAG 4 cut(s) 325, 699, 810, 1271
NmuCI GTSAC 3 cut(s) 429, 439, 2110
NspI RCATGY 1 cut(s) 404
NspV TTCGAA 1 cut(s) 2135
PdiI GCCGGC 1 cut(s) 531
PdmI GAANNNNTTC 2 cut(s) 260, 1192
PfeI GAWTC 3 cut(s) 205, 1434, 1567
PflFI GACNNNGTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 426
PfoI TCCNGGA 3 cut(s) 1682, 2067, 2268
PleI GAGTC 7 cut(s) 148, 332, 381, 446, 887, 1761, 1820
PluTI GGCGCC 1 cut(s) 785
PpsI GAGTC 7 cut(s) 148, 332, 381, 446, 887, 1761, 1820
PpuMI RGGWCCY 1 cut(s) 696
Psp124BI GAGCTC 2 cut(s) 360, 1217
Psp1406I AACGTT 1 cut(s) 2118
Psp5II RGGWCCY 1 cut(s) 696
Psp6I CCWGG 2 cut(s) 1037, 1591
PspFI CCCAGC 1 cut(s) 816
PspGI CCWGG 2 cut(s) 1037, 1591
PspN4I GGNNCC 6 cut(s) 697, 698, 783, 1011, 1590, 1742
PspPI GGNCC 6 cut(s) 532, 696, 1004, 1544, 1631, 2123
PspPPI RGGWCCY 1 cut(s) 696
PstNI CAGNNNCTG 2 cut(s) 1088, 2224
PsuI RGATCY 1 cut(s) 1009
PsyI GACNNNGTC 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 1358
RsaI GTAC 5 cut(s) 167, 189, 353, 806, 1994
RsaNI GTAC 5 cut(s) 166, 188, 352, 805, 1993
RseI CAYNNNNRTG 2 cut(s) 515, 624
SacI GAGCTC 2 cut(s) 360, 1217
SaqAI TTAA 3 cut(s) 38, 666, 1599
Sau3AI GATC 8 cut(s) 226, 645, 679, 1009, 1240, 1341, 1651, 1858
Sau96I GGNCC 6 cut(s) 532, 696, 1004, 1544, 1631, 2123
ScaI AGTACT 1 cut(s) 1994
SchI GAGTC 7 cut(s) 149, 333, 382, 447, 888, 1762, 1820
SduI GDGCHC 6 cut(s) 360, 393, 1217, 1875, 1894, 2307
SfcI CTRYAG 1 cut(s) 1132
SfoI GGCGCC 1 cut(s) 783
SfuI TTCGAA 1 cut(s) 2135
SinI GGWCC 1 cut(s) 696
SmaI CCCGGG 2 cut(s) 1902, 2032
SmiMI CAYNNNNRTG 2 cut(s) 515, 624
SmlI CTYRAG 3 cut(s) 1562, 1571, 1730
SmoI CTYRAG 3 cut(s) 1562, 1571, 1730
SpeI ACTAGT 1 cut(s) 1199
Sse9I AATT 9 cut(s) 594, 609, 795, 972, 1192, 1842, 1940, 2137, 2201
SspDI GGCGCC 1 cut(s) 781
SspMI CTAG 6 cut(s) 308, 1059, 1200, 1293, 1494, 2021
SstI GAGCTC 2 cut(s) 360, 1217
StyI CCWWGG 1 cut(s) 1065
TaiI ACGT 4 cut(s) 456, 1238, 1665, 2121
TaqI TCGA 4 cut(s) 393, 1997, 2135, 2184
TasI AATT 9 cut(s) 594, 609, 795, 972, 1192, 1842, 1940, 2137, 2201
TatI WGTACW 1 cut(s) 1992
TauI GCSGC 8 cut(s) 307, 710, 803, 828, 950, 1613, 1716, 1724
TfiI GAWTC 3 cut(s) 205, 1434, 1567
Tru1I TTAA 3 cut(s) 38, 666, 1599
Tru9I TTAA 3 cut(s) 38, 666, 1599
TscAI CASTG 4 cut(s) 517, 531, 542, 709
TseFI GTSAC 3 cut(s) 429, 439, 2110
TseI GCWGC 8 cut(s) 275, 632, 828, 1358, 1526, 1760, 1835, 2338
Tsp45I GTSAC 3 cut(s) 429, 439, 2110
TspDTI ATGAA 4 cut(s) 17, 43, 1160, 1524
TspGWI ACGGA 7 cut(s) 175, 231, 336, 636, 674, 931, 1380
TspMI CCCGGG 2 cut(s) 1900, 2030
TspRI CASTG 4 cut(s) 517, 531, 542, 709
Tth111I GACNNNGTC 1 cut(s) 323
Van91I CCANNNNNTGG 1 cut(s) 426
VneI GTGCAC 1 cut(s) 2303
VpaK11BI GGWCC 1 cut(s) 696
XapI RAATTY 4 cut(s) 609, 972, 1940, 2201
XceI RCATGY 1 cut(s) 404
XmaI CCCGGG 2 cut(s) 1900, 2030
XmnI GAANNNNTTC 2 cut(s) 260, 1192
XspI CTAG 6 cut(s) 308, 1059, 1200, 1293, 1494, 2021
ZraI GACGTC 1 cut(s) 454
ZrmI AGTACT 1 cut(s) 1994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.