FvH4_7g29280

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013721
Physical Location & Seq
Forward (+)
21583585 .. 21584922
1338 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g29280.t1

Sequence Viewer

Length: 1338 bp
ATGCATACCAAATTCAAGAGGGTGGCATCTACAACAGACCCTAGGCATCAACAAGGCCTCCTACCTTCAGCACATCGTCAATGGGAGTCTCTTGATCTGGTTGTGGTGTTTGTGGTACTTGACAAGTTGTTGGAACCCATCGACCTCGTTAGATTCGGCGCGGTTTGCAAACAATGGTCTCGGTTTGCACAAGACTACAAGCAATGGTACAAGCGAGCGGCGCTACCCATGCTCCTAATTCCTATCGACTACAATGAATCAAGTCAGGAATGGCGTTACGGAAAGAAGAACAGGCAACCAAAGCACCAAGGATTGCTGTACAGCGTTTCGGAACGAAGAGTCTACAGCAATGTGCAGAGTATTGTGCTGCCATCAGTTAGCAAACCAAGAAGCAATACACAGTGTAGAAGATACTATCGTGGATGTGGGCATGGTTGGGTGGCCATGATAGACAAGGATGTGGATGGAACTCATGGAAGCGGCGTGATTATAACTCTGGTCAACCCTTTCACAACAGCAATGTTGCAGTTGGTTAGTACTTCAATTTTCTATGGCATCCCGAAAGTTATATCGTTGGGAGATCCGACTTCGAATTCAGGTGTGCAATTAATTTTCTGTGATGAGATGTCCTGGATTCAAGTCAGAGGAAGACAACGACAAGCATGGAAAACTTGCCATCCACATTACCTCTTAGATGATCCAAATGACCTCTTACATCATGTGTGCAAATTTACTGATGCTATATTTTACAGAGGCCAAATCTACATGGCTGATTGTAGTAATTGCGGAATTGAGGTGATTGATTATGAAGATCTAGACCAAAACAAAATAGTATACGCATCACCTCCTTCAGCTGTTGTGGATTCCCTAAATTTTCCACAACGAGCTGAACGAGCTTATCTTGTGGAATCAATCAAGGGAGACTTGTTCTATATTCGAAGGTTTACAAAGTTAAACAGTATTCAAATGGTGACTGAGAGTTTTAGGGTTTATAAGGTAGTGCTCCATGGATCCATTGTGGAGTATGTTGAGGTGAAAAGCATTGGAGATGAGGCTTTCTTTGTTGGTGATAGTCCTTCGGTATCTGTTTTGGCTTCAAGCTCTTCAGGGTGTCAACCAAATTCGATTTACTTTACGGACGATTCGATAAATTATTCTCTATATTCGGCTAATTACTTTGATGAGAATCTACAAGTCGAAAACCAGAATAGTGAAGTACAAGTTGATATGGGTGTCTTCCATTTAGAGGATGAAACTATTAGCCAACATTACCCAAAATATAGTTTTGATGGAAGGCTGCTACCACCGCCGTTTTGGATTATGCCGCCGGCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

446

Amino Acids

50.93

Weight (kDa)

6.36

Isoelectric Point (pI)

43.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 123 - 388 8.5e-36 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 491, 993
AccBSI CCGCTC 1 cut(s) 218
AccI GTMKAC 2 cut(s) 342, 834
AccII CGCG 1 cut(s) 161
AciI CCGC 6 cut(s) 161, 218, 480, 786, 1307, 1325
AclWI GGATC 4 cut(s) 575, 692, 1005, 1018
AcoI YGGCCR 2 cut(s) 441, 1329
AcsI RAATTY 5 cut(s) 11, 592, 728, 871, 1120
AcuI CTGAAG 3 cut(s) 51, 834, 1089
AfaI GTAC 5 cut(s) 117, 209, 320, 538, 1218
AfiI CCNNNNNNNGG 1 cut(s) 1246
AgsI TTSAA 5 cut(s) 16, 543, 638, 965, 1098
AjnI CCWGG 1 cut(s) 629
AluBI AGCT 4 cut(s) 854, 887, 896, 1101
AluI AGCT 4 cut(s) 854, 887, 896, 1101
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 3 cut(s) 93, 183, 915
AlwI GGATC 4 cut(s) 575, 692, 1005, 1018
AoxI GGCC 4 cut(s) 55, 441, 754, 1329
ApeKI GCWGC 2 cut(s) 367, 1297
ApoI RAATTY 5 cut(s) 11, 592, 728, 871, 1120
AseI ATTAAT 1 cut(s) 608
AspA2I CCTAGG 1 cut(s) 41
AspLEI GCGC 2 cut(s) 161, 223
AsuHPI GGTGA 5 cut(s) 808, 834, 982, 1045, 1079
AsuII TTCGAA 2 cut(s) 590, 937
AvrII CCTAGG 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 403, 436
BalI TGGCCA 1 cut(s) 443
BamHI GGATCC 1 cut(s) 1010
BbsI GAAGAC 2 cut(s) 655, 1228
Bbv12I GWGCWC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 354, 1284
BccI CCATC 5 cut(s) 146, 379, 458, 684, 1283
BceAI ACGGC 2 cut(s) 1294, 1316
BciT130I CCWGG 1 cut(s) 631
BcoDI GTCTC 3 cut(s) 93, 183, 915
BfaI CTAG 2 cut(s) 42, 815
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 224
BglII AGATCT 1 cut(s) 811
BisI GCNGC 5 cut(s) 219, 368, 481, 1298, 1325
BlnI CCTAGG 1 cut(s) 41
BlsI GCNGC 5 cut(s) 220, 369, 482, 1299, 1326
BmcAI AGTACT 1 cut(s) 538
Bme1390I CCNGG 1 cut(s) 631
BmiI GGNNCC 2 cut(s) 135, 1012
BmrFI CCNGG 1 cut(s) 631
BmsI GCATC 5 cut(s) 35, 55, 564, 727, 848
BpiI GAAGAC 2 cut(s) 655, 1228
Bpu14I TTCGAA 2 cut(s) 590, 937
BsaBI GATNNNNATC 1 cut(s) 1185
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 3 cut(s) 41, 307, 1006
BsaXI ACNNNNNCTCC 4 cut(s) 42, 72, 216, 246
Bsc4I CCNNNNNNNGG 1 cut(s) 1246
Bse118I RCCGGY 1 cut(s) 1327
Bse3DI GCAATG 3 cut(s) 209, 355, 525
Bse8I GATNNNNATC 1 cut(s) 1185
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 3 cut(s) 41, 307, 1006
BseGI GGATG 6 cut(s) 428, 463, 469, 555, 676, 1255
BseJI GATNNNNATC 1 cut(s) 1185
BseLI CCNNNNNNNGG 1 cut(s) 1246
BseMI GCAATG 3 cut(s) 209, 355, 525
BseMII CTCAG 1 cut(s) 966
BseX3I CGGCCG 1 cut(s) 1329
BseXI GCAGC 2 cut(s) 354, 1284
BsgI GTGCAG 1 cut(s) 374
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 1332
BshFI GGCC 4 cut(s) 57, 443, 756, 1331
BsiEI CGRYCG 1 cut(s) 1332
BsiHKAI GWGCWC 1 cut(s) 1005
BsiSI CCGG 1 cut(s) 1328
BslI CCNNNNNNNGG 1 cut(s) 1246
BsmAI GTCTC 3 cut(s) 93, 183, 915
BsnI GGCC 4 cut(s) 57, 443, 756, 1331
Bso31I GGTCTC 1 cut(s) 183
Bsp119I TTCGAA 2 cut(s) 590, 937
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 5 cut(s) 94, 580, 697, 811, 1010
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 6 cut(s) 161, 218, 480, 786, 1307, 1325
BspANI GGCC 4 cut(s) 57, 443, 756, 1331
BspCNI CTCAG 1 cut(s) 967
BspFNI CGCG 1 cut(s) 161
BspLI GGNNCC 2 cut(s) 135, 1012
BspPI GGATC 4 cut(s) 575, 692, 1005, 1018
BspQI GCTCTTC 1 cut(s) 1108
BspT104I TTCGAA 2 cut(s) 590, 937
BspTNI GGTCTC 1 cut(s) 183
BsrBI CCGCTC 1 cut(s) 218
BsrDI GCAATG 3 cut(s) 209, 355, 525
BsrFI RCCGGY 1 cut(s) 1327
BsrGI TGTACA 1 cut(s) 318
BssAI RCCGGY 1 cut(s) 1327
BssECI CCNNGG 3 cut(s) 41, 307, 1006
BssMI GATC 5 cut(s) 94, 580, 697, 811, 1010
BssNAI GTATAC 1 cut(s) 835
BssT1I CCWWGG 3 cut(s) 41, 307, 1006
Bst1107I GTATAC 1 cut(s) 835
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 2 cut(s) 402, 959
Bst6I CTCTTC 2 cut(s) 331, 1108
BstAUI TGTACA 1 cut(s) 318
BstBI TTCGAA 2 cut(s) 590, 937
BstC8I GCNNGC 2 cut(s) 216, 1329
BstDEI CTNAG 2 cut(s) 691, 975
BstDSI CCRYGG 1 cut(s) 1006
BstF5I GGATG 6 cut(s) 428, 463, 469, 555, 676, 1255
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 2 cut(s) 161, 223
BstKTI GATC 5 cut(s) 97, 583, 700, 814, 1013
BstMAI GTCTC 3 cut(s) 93, 183, 915
BstMBI GATC 5 cut(s) 94, 580, 697, 811, 1010
BstMCI CGRYCG 1 cut(s) 1332
BstMWI GCNNNNNNNGC 6 cut(s) 165, 220, 229, 301, 893, 1306
BstNI CCWGG 1 cut(s) 631
BstSCI CCNGG 1 cut(s) 629
BstSFI CTRYAG 1 cut(s) 343
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 354, 1284
BstV2I GAAGAC 2 cut(s) 655, 1228
BstX2I RGATCY 3 cut(s) 580, 811, 1010
BstYI RGATCY 3 cut(s) 580, 811, 1010
BstZ17I GTATAC 1 cut(s) 835
BstZI CGGCCG 1 cut(s) 1329
BsuRI GGCC 4 cut(s) 57, 443, 756, 1331
BtgI CCRYGG 1 cut(s) 1006
BtsCI GGATG 6 cut(s) 428, 463, 469, 555, 676, 1255
BtsIMutI CAGTG 1 cut(s) 407
Cac8I GCNNGC 2 cut(s) 216, 1329
CfoI GCGC 2 cut(s) 161, 223
Cfr10I RCCGGY 1 cut(s) 1327
Csp6I GTAC 5 cut(s) 116, 208, 319, 537, 1217
CviAII CATG 8 cut(s) 229, 431, 445, 473, 663, 719, 766, 1007
CviQI GTAC 5 cut(s) 116, 208, 319, 537, 1217
DdeI CTNAG 2 cut(s) 691, 975
DpnI GATC 5 cut(s) 96, 582, 699, 813, 1012
DpnII GATC 5 cut(s) 94, 580, 697, 811, 1010
EaeI YGGCCR 2 cut(s) 441, 1329
EagI CGGCCG 1 cut(s) 1329
Eam1104I CTCTTC 2 cut(s) 331, 1108
EarI CTCTTC 2 cut(s) 331, 1108
EclXI CGGCCG 1 cut(s) 1329
Eco130I CCWWGG 3 cut(s) 41, 307, 1006
Eco147I AGGCCT 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 183
Eco52I CGGCCG 1 cut(s) 1329
Eco57I CTGAAG 3 cut(s) 51, 834, 1089
EcoRI GAATTC 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 629
EcoT14I CCWWGG 3 cut(s) 41, 307, 1006
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 41, 307, 1006
FaeI CATG 8 cut(s) 232, 434, 448, 476, 666, 722, 769, 1010
FalI AAGNNNNNCTT 2 cut(s) 908, 940
FatI CATG 8 cut(s) 228, 430, 444, 472, 662, 718, 765, 1006
FblI GTMKAC 2 cut(s) 342, 834
Fnu4HI GCNGC 5 cut(s) 219, 368, 481, 1298, 1325
FokI GGATG 6 cut(s) 435, 470, 476, 542, 663, 1262
Fsp4HI GCNGC 5 cut(s) 219, 368, 481, 1298, 1325
FspBI CTAG 2 cut(s) 42, 815
GlaI GCGC 2 cut(s) 160, 222
GluI GCNGC 5 cut(s) 219, 368, 481, 1298, 1325
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 57, 443, 756, 1331
HapII CCGG 1 cut(s) 1328
HhaI GCGC 2 cut(s) 161, 223
Hin1II CATG 8 cut(s) 232, 434, 448, 476, 666, 722, 769, 1010
Hin6I GCGC 2 cut(s) 159, 221
HinP1I GCGC 2 cut(s) 159, 221
HincII GTYRAC 2 cut(s) 502, 1115
HindII GTYRAC 2 cut(s) 502, 1115
HinfI GANTC 9 cut(s) 86, 153, 257, 339, 634, 863, 908, 1142, 1186
HpaII CCGG 1 cut(s) 1328
HphI GGTGA 5 cut(s) 808, 834, 982, 1045, 1079
Hpy166II GTNNAC 5 cut(s) 343, 502, 835, 945, 1115
Hpy188I TCNGA 3 cut(s) 331, 585, 644
Hpy188III TCNNGA 5 cut(s) 16, 92, 266, 559, 815
Hpy8I GTNNAC 5 cut(s) 343, 502, 835, 945, 1115
HpyAV CCTTC 5 cut(s) 75, 858, 933, 1086, 1287
HpyCH4III ACNGT 2 cut(s) 402, 959
HpyCH4V TGCA 7 cut(s) 4, 168, 188, 355, 526, 604, 726
HpyF10VI GCNNNNNNNGC 6 cut(s) 165, 220, 229, 301, 893, 1306
HpyF3I CTNAG 2 cut(s) 691, 975
Hsp92II CATG 8 cut(s) 232, 434, 448, 476, 666, 722, 769, 1010
HspAI GCGC 2 cut(s) 159, 221
KroI GCCGGC 1 cut(s) 1327
KroNI GCCGGC 1 cut(s) 1329
Kzo9I GATC 5 cut(s) 94, 580, 697, 811, 1010
LguI GCTCTTC 1 cut(s) 1108
LmnI GCTCC 2 cut(s) 237, 1008
LpnPI CCDG 9 cut(s) 83, 251, 277, 482, 582, 616, 643, 1092, 1217
Lsp1109I GCAGC 2 cut(s) 354, 1284
LweI GCATC 5 cut(s) 35, 55, 564, 727, 848
MaeI CTAG 2 cut(s) 42, 815
MaeIII GTNAC 2 cut(s) 275, 970
MalI GATC 5 cut(s) 96, 582, 699, 813, 1012
MbiI CCGCTC 1 cut(s) 218
MboI GATC 5 cut(s) 94, 580, 697, 811, 1010
MboII GAAGA 7 cut(s) 298, 348, 420, 660, 821, 1095, 1228
MflI RGATCY 3 cut(s) 580, 811, 1010
MhlI GDGCHC 1 cut(s) 1005
MlsI TGGCCA 1 cut(s) 443
MluNI TGGCCA 1 cut(s) 443
MlyI GAGTC 2 cut(s) 95, 348
MmeI TCCRAC 2 cut(s) 111, 608
Mox20I TGGCCA 1 cut(s) 443
Mph1103I ATGCAT 1 cut(s) 6
MroNI GCCGGC 1 cut(s) 1327
MscI TGGCCA 1 cut(s) 443
MseI TTAA 3 cut(s) 608, 953, 1336
Msp20I TGGCCA 1 cut(s) 443
MspA1I CMGCKG 1 cut(s) 854
MspI CCGG 1 cut(s) 1328
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 6 cut(s) 165, 220, 229, 301, 893, 1306
NaeI GCCGGC 1 cut(s) 1329
NcoI CCATGG 1 cut(s) 1006
NdeII GATC 5 cut(s) 94, 580, 697, 811, 1010
NgoMIV GCCGGC 1 cut(s) 1327
NlaIII CATG 8 cut(s) 232, 434, 448, 476, 666, 722, 769, 1010
NlaIV GGNNCC 2 cut(s) 135, 1012
NmuCI GTSAC 1 cut(s) 970
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 2 cut(s) 590, 937
PceI AGGCCT 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 1108
PcsI WCGNNNNNNNCGW 2 cut(s) 889, 1142
PdiI GCCGGC 1 cut(s) 1329
PfeI GAWTC 7 cut(s) 153, 257, 634, 863, 908, 1142, 1186
PfoI TCCNGGA 1 cut(s) 629
PkrI GCNGC 5 cut(s) 220, 369, 482, 1299, 1326
PleI GAGTC 2 cut(s) 94, 347
PpsI GAGTC 2 cut(s) 94, 347
PshBI ATTAAT 1 cut(s) 608
PsiI TTATAA 2 cut(s) 491, 993
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 2 cut(s) 135, 1012
PsuI RGATCY 3 cut(s) 580, 811, 1010
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 5 cut(s) 117, 209, 320, 538, 1218
RsaNI GTAC 5 cut(s) 116, 208, 319, 537, 1217
SapI GCTCTTC 1 cut(s) 1108
SaqAI TTAA 3 cut(s) 608, 953, 1336
SatI GCNGC 5 cut(s) 219, 368, 481, 1298, 1325
Sau3AI GATC 5 cut(s) 94, 580, 697, 811, 1010
ScaI AGTACT 1 cut(s) 538
SchI GAGTC 2 cut(s) 95, 348
ScrFI CCNGG 1 cut(s) 631
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 5 cut(s) 35, 55, 564, 727, 848
SfcI CTRYAG 1 cut(s) 343
SfuI TTCGAA 2 cut(s) 590, 937
SseBI AGGCCT 1 cut(s) 57
SsiI CCGC 6 cut(s) 161, 218, 480, 786, 1307, 1325
SspMI CTAG 2 cut(s) 42, 815
StuI AGGCCT 1 cut(s) 57
StyD4I CCNGG 1 cut(s) 629
StyI CCWWGG 3 cut(s) 41, 307, 1006
TaaI ACNGT 2 cut(s) 402, 959
TaqI TCGA 7 cut(s) 141, 246, 590, 937, 1124, 1145, 1197
TatI WGTACW 3 cut(s) 318, 536, 1216
TauI GCSGC 3 cut(s) 221, 483, 1327
TfiI GAWTC 7 cut(s) 153, 257, 634, 863, 908, 1142, 1186
Tru1I TTAA 3 cut(s) 608, 953, 1336
Tru9I TTAA 3 cut(s) 608, 953, 1336
TscAI CASTG 1 cut(s) 407
TseFI GTSAC 1 cut(s) 970
TseI GCWGC 2 cut(s) 367, 1297
Tsp45I GTSAC 1 cut(s) 970
TspDTI ATGAA 3 cut(s) 270, 822, 1266
TspGWI ACGGA 2 cut(s) 294, 1151
TspRI CASTG 1 cut(s) 407
VspI ATTAAT 1 cut(s) 608
XapI RAATTY 5 cut(s) 11, 592, 728, 871, 1120
XbaI TCTAGA 1 cut(s) 814
XcmI CCANNNNNNNNNTGG 1 cut(s) 1311
XmaJI CCTAGG 1 cut(s) 41
XmiI GTMKAC 2 cut(s) 342, 834
XspI CTAG 2 cut(s) 42, 815
ZrmI AGTACT 1 cut(s) 538
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.