Rh1AG417900

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0013721
Physical Location & Seq
Forward (+)
64959067 .. 64960401
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG417900.1

Sequence Viewer

Length: 735 bp
ATGCATACCAAATTCGATAGGGTGGTATCTACAATAAACCCTAGGCTCCAACAAGGCTTACCACACATAAGAGCTAGTGAGGTACTCAAAGATTCAGATTCAATGCATCATCAATGGGAATGTCTTGATCTGGACGTGGTGTTTTTGGTTCTTGATAAATTGTTGGAACCCATCGACCGTGTTAGTTTCGGGGTAGTTTGTAAAGAATGGGGTCGTCTTTCAAGAGCCTATAAGCGATGGTGTGAGCGAAAGGTGCTCCCCATGCTTCTAGTCCCTAGCGACTACGATGAATCACGTCAGGAATGGCGTTATGGAAAGAAGAACAGACGACCAAAGCACCAAGGATTACTGTACAGCATTTTGGAACGAAGAATCTACAGCCATGTGCAGAGTCTTCTGCTCCCATCAGTAAGTAAACCAAGACGTGGATGTAGCAGATACTATCGTGGATGTGGTCATGGTTGGGTGGCCATGATAGACATGGATGAGTTTAGAAGGGAAGTGATTATAACTCTCGTCAACCCCTTCACAACTGCAATGTTGCAGCGTTTCCCTCCATTGGAAATCTATGGCTGGGGTATCCCAAAAGTTATCTTGTCCGAAGATCCGACTTTGAATCAAGACAATTATGTGGTTGTAGCTTTATCTGATCATGACTCCTTGGATTTCATCAAAGGAAGACAAGATTGTAAGTTGCTACCACCCGCTTTTTGGATTATACCACCGGCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

244

Amino Acids

28.56

Weight (kDa)

9.0

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 142 - 219 2.9e-08 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 425
AciI CCGC 1 cut(s) 705
AclWI GGATC 1 cut(s) 599
AcoI YGGCCR 2 cut(s) 468, 726
AcsI RAATTY 1 cut(s) 11
AfaI GTAC 2 cut(s) 84, 353
AfiI CCNNNNNNNGG 3 cut(s) 425, 559, 711
AgsI TTSAA 3 cut(s) 102, 222, 616
AjiI CACGTC 3 cut(s) 136, 296, 425
AluBI AGCT 2 cut(s) 74, 641
AluI AGCT 2 cut(s) 74, 641
Alw21I GWGCWC 1 cut(s) 258
AlwI GGATC 1 cut(s) 599
AoxI GGCC 2 cut(s) 468, 726
ApeKI GCWGC 1 cut(s) 544
ApoI RAATTY 1 cut(s) 11
AspA2I CCTAGG 1 cut(s) 41
AvrII CCTAGG 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BalI TGGCCA 1 cut(s) 470
BbsI GAAGAC 2 cut(s) 386, 685
Bbv12I GWGCWC 1 cut(s) 258
BbvI GCAGC 1 cut(s) 556
BccI CCATC 3 cut(s) 179, 231, 412
BceAI ACGGC 1 cut(s) 713
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 1 cut(s) 649
BfaI CTAG 4 cut(s) 42, 75, 269, 276
BfmI CTRYAG 1 cut(s) 376
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 1 cut(s) 545
BlnI CCTAGG 1 cut(s) 41
BlsI GCNGC 1 cut(s) 546
BmgBI CACGTC 3 cut(s) 136, 296, 425
BmiI GGNNCC 2 cut(s) 47, 168
BmsI GCATC 1 cut(s) 115
BpiI GAAGAC 2 cut(s) 386, 685
BsaJI CCNNGG 3 cut(s) 41, 340, 660
Bsc4I CCNNNNNNNGG 3 cut(s) 425, 559, 711
Bse118I RCCGGY 1 cut(s) 724
Bse3DI GCAATG 1 cut(s) 543
BseDI CCNNGG 3 cut(s) 41, 340, 660
BseGI GGATG 3 cut(s) 434, 455, 490
BseLI CCNNNNNNNGG 3 cut(s) 425, 559, 711
BseMI GCAATG 1 cut(s) 543
BseX3I CGGCCG 1 cut(s) 726
BseXI GCAGC 1 cut(s) 556
BseYI CCCAGC 1 cut(s) 573
BsgI GTGCAG 1 cut(s) 407
Bsh1285I CGRYCG 2 cut(s) 178, 729
BshFI GGCC 2 cut(s) 470, 728
BsiEI CGRYCG 2 cut(s) 178, 729
BsiHKAI GWGCWC 1 cut(s) 258
BsiSI CCGG 1 cut(s) 725
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 3 cut(s) 425, 559, 711
BsmFI GGGAC 1 cut(s) 257
BsnI GGCC 2 cut(s) 470, 728
Bsp1286I GDGCHC 1 cut(s) 258
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 3 cut(s) 127, 604, 649
BspACI CCGC 1 cut(s) 705
BspANI GGCC 2 cut(s) 470, 728
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 2 cut(s) 47, 168
BspPI GGATC 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 543
BsrFI RCCGGY 1 cut(s) 724
BsrGI TGTACA 1 cut(s) 351
BssAI RCCGGY 1 cut(s) 724
BssECI CCNNGG 3 cut(s) 41, 340, 660
BssMI GATC 3 cut(s) 127, 604, 649
BssT1I CCWWGG 3 cut(s) 41, 340, 660
Bst4CI ACNGT 2 cut(s) 179, 351
BstAUI TGTACA 1 cut(s) 351
BstF5I GGATG 3 cut(s) 434, 455, 490
BstKTI GATC 3 cut(s) 130, 607, 652
BstMBI GATC 3 cut(s) 127, 604, 649
BstMCI CGRYCG 2 cut(s) 178, 729
BstMWI GCNNNNNNNGC 2 cut(s) 253, 262
BstSFI CTRYAG 1 cut(s) 376
BstV1I GCAGC 1 cut(s) 556
BstV2I GAAGAC 2 cut(s) 386, 685
BstX2I RGATCY 1 cut(s) 604
BstYI RGATCY 1 cut(s) 604
BstZI CGGCCG 1 cut(s) 726
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 470, 728
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 3 cut(s) 136, 296, 425
BtsCI GGATG 3 cut(s) 434, 455, 490
CciI TCATGA 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 724
Csp6I GTAC 2 cut(s) 83, 352
CviAII CATG 6 cut(s) 262, 383, 458, 472, 481, 653
CviJI RGCY 9 cut(s) 46, 57, 74, 227, 381, 470, 573, 641, 728
CviKI_1 RGCY 9 cut(s) 46, 57, 74, 227, 381, 470, 573, 641, 728
CviQI GTAC 2 cut(s) 83, 352
DpnI GATC 3 cut(s) 129, 606, 651
DpnII GATC 3 cut(s) 127, 604, 649
EaeI YGGCCR 2 cut(s) 468, 726
EagI CGGCCG 1 cut(s) 726
EclXI CGGCCG 1 cut(s) 726
Eco130I CCWWGG 3 cut(s) 41, 340, 660
Eco52I CGGCCG 1 cut(s) 726
EcoT14I CCWWGG 3 cut(s) 41, 340, 660
EcoT22I ATGCAT 2 cut(s) 6, 108
ErhI CCWWGG 3 cut(s) 41, 340, 660
FaeI CATG 6 cut(s) 265, 386, 461, 475, 484, 656
FaqI GGGAC 1 cut(s) 257
FatI CATG 6 cut(s) 261, 382, 457, 471, 480, 652
FauI CCCGC 1 cut(s) 712
FbaI TGATCA 1 cut(s) 649
Fnu4HI GCNGC 1 cut(s) 545
FokI GGATG 3 cut(s) 441, 462, 497
Fsp4HI GCNGC 1 cut(s) 545
FspBI CTAG 4 cut(s) 42, 75, 269, 276
GluI GCNGC 1 cut(s) 545
GsaI CCCAGC 1 cut(s) 577
HaeIII GGCC 2 cut(s) 470, 728
HapII CCGG 1 cut(s) 725
Hin1II CATG 6 cut(s) 265, 386, 461, 475, 484, 656
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 7 cut(s) 92, 98, 290, 372, 391, 616, 656
HpaII CCGG 1 cut(s) 725
Hpy166II GTNNAC 2 cut(s) 416, 520
Hpy188I TCNGA 4 cut(s) 97, 601, 609, 649
Hpy188III TCNNGA 7 cut(s) 125, 131, 152, 222, 299, 620, 653
Hpy8I GTNNAC 2 cut(s) 416, 520
HpyAV CCTTC 2 cut(s) 489, 535
HpyCH4III ACNGT 2 cut(s) 179, 351
HpyCH4IV ACGT 3 cut(s) 135, 295, 424
HpyCH4V TGCA 5 cut(s) 4, 106, 388, 536, 544
HpyF10VI GCNNNNNNNGC 2 cut(s) 253, 262
HpySE526I ACGT 3 cut(s) 135, 295, 424
Hsp92II CATG 6 cut(s) 265, 386, 461, 475, 484, 656
Ksp22I TGATCA 1 cut(s) 649
Kzo9I GATC 3 cut(s) 127, 604, 649
LmnI GCTCC 3 cut(s) 51, 261, 405
LpnPI CCDG 3 cut(s) 116, 284, 559
Lsp1109I GCAGC 1 cut(s) 556
LweI GCATC 1 cut(s) 115
MaeI CTAG 4 cut(s) 42, 75, 269, 276
MaeII ACGT 3 cut(s) 135, 295, 424
MalI GATC 3 cut(s) 129, 606, 651
MboI GATC 3 cut(s) 127, 604, 649
MboII GAAGA 5 cut(s) 331, 381, 386, 614, 690
MflI RGATCY 1 cut(s) 604
MhlI GDGCHC 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 3 cut(s) 11, 158, 625
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 400, 650
MmeI TCCRAC 3 cut(s) 73, 144, 632
MnlI CCTC 2 cut(s) 73, 564
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 2 cut(s) 6, 108
MscI TGGCCA 1 cut(s) 470
MseI TTAA 1 cut(s) 733
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 1 cut(s) 725
MwoI GCNNNNNNNGC 2 cut(s) 253, 262
NdeII GATC 3 cut(s) 127, 604, 649
NlaIII CATG 6 cut(s) 265, 386, 461, 475, 484, 656
NlaIV GGNNCC 2 cut(s) 47, 168
NsiI ATGCAT 2 cut(s) 6, 108
PagI TCATGA 1 cut(s) 652
PfeI GAWTC 5 cut(s) 92, 98, 290, 372, 616
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 1 cut(s) 546
PleI GAGTC 2 cut(s) 399, 650
PpsI GAGTC 2 cut(s) 399, 650
PsiI TTATAA 1 cut(s) 509
PspFI CCCAGC 1 cut(s) 573
PspN4I GGNNCC 2 cut(s) 47, 168
PsuI RGATCY 1 cut(s) 604
RsaI GTAC 2 cut(s) 84, 353
RsaNI GTAC 2 cut(s) 83, 352
SaqAI TTAA 1 cut(s) 733
SatI GCNGC 1 cut(s) 545
Sau3AI GATC 3 cut(s) 127, 604, 649
SchI GAGTC 2 cut(s) 400, 650
SduI GDGCHC 1 cut(s) 258
SetI ASST 7 cut(s) 76, 84, 138, 255, 298, 427, 643
SfaNI GCATC 1 cut(s) 115
SfcI CTRYAG 1 cut(s) 376
Sse9I AATT 3 cut(s) 11, 158, 625
SsiI CCGC 1 cut(s) 705
SspMI CTAG 4 cut(s) 42, 75, 269, 276
StyI CCWWGG 3 cut(s) 41, 340, 660
TaaI ACNGT 2 cut(s) 179, 351
TaiI ACGT 3 cut(s) 138, 298, 427
TaqI TCGA 2 cut(s) 15, 174
TasI AATT 3 cut(s) 11, 158, 625
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 5 cut(s) 92, 98, 290, 372, 616
Tru1I TTAA 1 cut(s) 733
Tru9I TTAA 1 cut(s) 733
TseI GCWGC 1 cut(s) 544
TspDTI ATGAA 2 cut(s) 303, 658
Van91I CCANNNNNTGG 1 cut(s) 425
XapI RAATTY 1 cut(s) 11
XcmI CCANNNNNNNNNTGG 2 cut(s) 478, 708
XmaJI CCTAGG 1 cut(s) 41
XspI CTAG 4 cut(s) 42, 75, 269, 276
Zsp2I ATGCAT 2 cut(s) 6, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.