Rroxscaffold_4G00281520

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013721
Physical Location & Seq
Reverse (-)
3790746 .. 3792080
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281520.1

Sequence Viewer

Length: 1335 bp
ATGCATACCAAATTCGATAGGGTGGTATCTACAATAAACCCTAGGCTCCAACACGGCTTACCACACATAAGAGCTAGTGAGGTACTCAAAGATTCAGATTCAATGCATCATCAATGGGAATGTCTTGATCTGGACGTGGTGTTTTTGGTTCTTGATAAGTTGTTGGAACCCATCGACCGTGTTAGTTTCGGGGTAGTTTGTAAAGAATGGGGTCGTCTTTCAAGAGCCTATAAGCGATGGTGTGAGCGAAAGGTGCTTCCCATGCTTCTAGTCCCTAGCGACTACGATGAATCACGTCAGGAATGGCGTTATGGAAAGAAGAACAGACGACCAAAGCACCAAGGATTACTGTACAGCATTTTGGAACGAAGAATCTACAGCCATGTGCAGAGTCTTCTGCTCCCATCAGTAAGTAAACCAAGACGTGGATGTAGCAGATACTATCGTGGATGTGGTCATGGTTGGGTGGCCATGATAGACATGGATGAGTTTAGAAGGGAAGTGATTATAACTCTCGTCAACCCCTTCACAACGGCAATGTTGCAGCGTTTCCCTCCATTGGAAATCTATGGCTGGGGTATCCCAAAAGTTATCTTGTCAGAAGATCCGACTTTGAATCAAGACAATTATGTGGTTGTAGCTTTATCTGATCATGACTCCTTGGATTTCATCAAAGGAAGACAAGATTGGTATCATATCGAATGCAAACCGTACCTCGCAGAGTTTGATCATTGCAAATTTACTGATGCTATATTTTACAGAGGCCAAATCTATATGGCTGATAATTGTTGCGGAATAATTGCGGTGTTCGATTACAAAAGCAAACAACAAATGTACTTCGAAAAGTTATGCACACCATTCCAAAATTTATCACAACGAGCTGAACGAGCATATCTTGTGCAATCAATCAAGGGAGACTTGTTGTATATTCGAAGATTTACAGCATTAAAGAGTTTAGACGAAGGCCATGTGACCGAGAATTTTAGGGTGTATAAGATGGTGCTCAATGGATCCATTGTGGAGCATGTTGAGGTAAAGAGCATTGGAGACGAGGCTTTGTTTGTTGGTGACAGTCATTCAATATCTTTATTGGCATCAAGCTCTTCAGGATCGTGTCAACCAAATTCGATTTACTTTACAGATGATTCGATAAATTTTTGCCTATATTTAGATAACTACGGCGGACTACAAGTCCTCGACAAGAATATTGAAGTACAAGTTGATATGGGCATCTTCCATTTAGAGGATGGAACTATTAGCCAATATTACCCAAAATATTCCTTTGACAGTAAGCTGCTACCACCCGCTTTTTGGATTATACCACCGGCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

444

Amino Acids

51.55

Weight (kDa)

6.57

Isoelectric Point (pI)

49.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 142 - 389 8e-41 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 425
AciI CCGC 4 cut(s) 792, 803, 1182, 1305
AclWI GGATC 4 cut(s) 599, 1005, 1018, 1117
AcoI YGGCCR 2 cut(s) 468, 1326
AcsI RAATTY 6 cut(s) 11, 737, 865, 979, 1123, 1153
AcuI CTGAAG 1 cut(s) 1089
AfaI GTAC 5 cut(s) 84, 353, 713, 836, 1215
AfiI CCNNNNNNNGG 4 cut(s) 425, 559, 1243, 1311
AgsI TTSAA 5 cut(s) 102, 222, 616, 1080, 1211
AhdI GACNNNNNGTC 1 cut(s) 1190
AjiI CACGTC 3 cut(s) 136, 296, 425
AjuI GAANNNNNNNTTGG 2 cut(s) 670, 702
AluBI AGCT 5 cut(s) 74, 641, 881, 1101, 1294
AluI AGCT 5 cut(s) 74, 641, 881, 1101, 1294
Alw21I GWGCWC 1 cut(s) 1005
Alw26I GTCTC 2 cut(s) 909, 1041
AlwI GGATC 4 cut(s) 599, 1005, 1018, 1117
AoxI GGCC 4 cut(s) 468, 763, 964, 1326
ApeKI GCWGC 2 cut(s) 544, 1294
ApoI RAATTY 6 cut(s) 11, 737, 865, 979, 1123, 1153
AspA2I CCTAGG 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 1079
AsuII TTCGAA 2 cut(s) 840, 931
AvrII CCTAGG 1 cut(s) 41
BaeI ACNNNNGTAYC 2 cut(s) 430, 463
BalI TGGCCA 1 cut(s) 470
BamHI GGATCC 1 cut(s) 1010
BbsI GAAGAC 2 cut(s) 386, 685
Bbv12I GWGCWC 1 cut(s) 1005
BbvI GCAGC 2 cut(s) 556, 1281
BccI CCATC 5 cut(s) 179, 231, 412, 991, 1241
BceAI ACGGC 4 cut(s) 70, 549, 1195, 1313
BciVI GTATCC 1 cut(s) 590
BclI TGATCA 2 cut(s) 649, 727
BcoDI GTCTC 2 cut(s) 909, 1041
BfaI CTAG 4 cut(s) 42, 75, 269, 276
BfmI CTRYAG 1 cut(s) 376
BfuI GTATCC 1 cut(s) 590
BisI GCNGC 2 cut(s) 545, 1295
BlnI CCTAGG 1 cut(s) 41
BlsI GCNGC 2 cut(s) 546, 1296
BmeRI GACNNNNNGTC 1 cut(s) 1190
BmgBI CACGTC 3 cut(s) 136, 296, 425
BmiI GGNNCC 3 cut(s) 47, 168, 1012
BmsI GCATC 4 cut(s) 115, 736, 1103, 1239
BpiI GAAGAC 2 cut(s) 386, 685
Bpu14I TTCGAA 2 cut(s) 840, 931
BsaBI GATNNNNATC 1 cut(s) 690
BsaJI CCNNGG 3 cut(s) 41, 340, 660
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 559, 1243, 1311
Bse118I RCCGGY 1 cut(s) 1324
Bse3DI GCAATG 2 cut(s) 543, 730
Bse8I GATNNNNATC 1 cut(s) 690
BseDI CCNNGG 3 cut(s) 41, 340, 660
BseGI GGATG 4 cut(s) 434, 455, 490, 1252
BseJI GATNNNNATC 1 cut(s) 690
BseLI CCNNNNNNNGG 4 cut(s) 425, 559, 1243, 1311
BseMI GCAATG 2 cut(s) 543, 730
BseX3I CGGCCG 1 cut(s) 1326
BseXI GCAGC 2 cut(s) 556, 1281
BseYI CCCAGC 1 cut(s) 573
BsgI GTGCAG 1 cut(s) 407
Bsh1285I CGRYCG 2 cut(s) 178, 1329
BshFI GGCC 4 cut(s) 470, 765, 966, 1328
BsiEI CGRYCG 2 cut(s) 178, 1329
BsiHKAI GWGCWC 1 cut(s) 1005
BsiSI CCGG 1 cut(s) 1325
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 4 cut(s) 425, 559, 1243, 1311
BsmAI GTCTC 2 cut(s) 909, 1041
BsmBI CGTCTC 1 cut(s) 1041
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 1 cut(s) 707
BsnI GGCC 4 cut(s) 470, 765, 966, 1328
Bsp119I TTCGAA 2 cut(s) 840, 931
Bsp1286I GDGCHC 1 cut(s) 1005
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
BspACI CCGC 4 cut(s) 792, 803, 1182, 1305
BspANI GGCC 4 cut(s) 470, 765, 966, 1328
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 3 cut(s) 47, 168, 1012
BspPI GGATC 4 cut(s) 599, 1005, 1018, 1117
BspQI GCTCTTC 1 cut(s) 1108
BspT104I TTCGAA 2 cut(s) 840, 931
BsrDI GCAATG 2 cut(s) 543, 730
BsrFI RCCGGY 1 cut(s) 1324
BsrGI TGTACA 1 cut(s) 351
BssAI RCCGGY 1 cut(s) 1324
BssECI CCNNGG 3 cut(s) 41, 340, 660
BssMI GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
BssT1I CCWWGG 3 cut(s) 41, 340, 660
Bst4CI ACNGT 5 cut(s) 179, 351, 711, 1073, 1289
Bst6I CTCTTC 1 cut(s) 1108
BstAUI TGTACA 1 cut(s) 351
BstBI TTCGAA 2 cut(s) 840, 931
BstF5I GGATG 4 cut(s) 434, 455, 490, 1252
BstKTI GATC 6 cut(s) 130, 607, 652, 730, 1013, 1112
BstMAI GTCTC 2 cut(s) 909, 1041
BstMBI GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
BstMCI CGRYCG 2 cut(s) 178, 1329
BstMWI GCNNNNNNNGC 3 cut(s) 253, 262, 887
BstNSI RCATGY 1 cut(s) 1028
BstSFI CTRYAG 1 cut(s) 376
BstV1I GCAGC 2 cut(s) 556, 1281
BstV2I GAAGAC 2 cut(s) 386, 685
BstX2I RGATCY 2 cut(s) 604, 1010
BstYI RGATCY 2 cut(s) 604, 1010
BstZI CGGCCG 1 cut(s) 1326
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 4 cut(s) 470, 765, 966, 1328
BtgZI GCGATG 1 cut(s) 250
BtrI CACGTC 3 cut(s) 136, 296, 425
BtsCI GGATG 4 cut(s) 434, 455, 490, 1252
CciI TCATGA 1 cut(s) 652
Cfr10I RCCGGY 1 cut(s) 1324
Csp6I GTAC 5 cut(s) 83, 352, 712, 835, 1214
CviAII CATG 8 cut(s) 262, 383, 458, 472, 481, 653, 968, 1025
CviQI GTAC 5 cut(s) 83, 352, 712, 835, 1214
DpnI GATC 6 cut(s) 129, 606, 651, 729, 1012, 1111
DpnII GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
DriI GACNNNNNGTC 1 cut(s) 1190
EaeI YGGCCR 2 cut(s) 468, 1326
EagI CGGCCG 1 cut(s) 1326
Eam1104I CTCTTC 1 cut(s) 1108
Eam1105I GACNNNNNGTC 1 cut(s) 1190
EarI CTCTTC 1 cut(s) 1108
EciI GGCGGA 1 cut(s) 1197
EclXI CGGCCG 1 cut(s) 1326
Eco130I CCWWGG 3 cut(s) 41, 340, 660
Eco52I CGGCCG 1 cut(s) 1326
Eco57I CTGAAG 1 cut(s) 1089
EcoT14I CCWWGG 3 cut(s) 41, 340, 660
EcoT22I ATGCAT 2 cut(s) 6, 108
ErhI CCWWGG 3 cut(s) 41, 340, 660
Esp3I CGTCTC 1 cut(s) 1041
FaeI CATG 8 cut(s) 265, 386, 461, 475, 484, 656, 971, 1028
FalI AAGNNNNNCTT 2 cut(s) 902, 934
FaqI GGGAC 1 cut(s) 257
FatI CATG 8 cut(s) 261, 382, 457, 471, 480, 652, 967, 1024
FauI CCCGC 1 cut(s) 1312
FbaI TGATCA 2 cut(s) 649, 727
Fnu4HI GCNGC 2 cut(s) 545, 1295
FokI GGATG 4 cut(s) 441, 462, 497, 1259
Fsp4HI GCNGC 2 cut(s) 545, 1295
FspBI CTAG 4 cut(s) 42, 75, 269, 276
GluI GCNGC 2 cut(s) 545, 1295
GsaI CCCAGC 1 cut(s) 577
HaeIII GGCC 4 cut(s) 470, 765, 966, 1328
HapII CCGG 1 cut(s) 1325
Hin1II CATG 8 cut(s) 265, 386, 461, 475, 484, 656, 971, 1028
HincII GTYRAC 2 cut(s) 520, 1118
HindII GTYRAC 2 cut(s) 520, 1118
HinfI GANTC 8 cut(s) 92, 98, 290, 372, 391, 616, 656, 1145
HpaII CCGG 1 cut(s) 1325
HphI GGTGA 1 cut(s) 1079
Hpy166II GTNNAC 3 cut(s) 416, 520, 1118
Hpy188I TCNGA 4 cut(s) 97, 601, 609, 649
Hpy188III TCNNGA 8 cut(s) 125, 131, 152, 222, 299, 620, 653, 1107
Hpy8I GTNNAC 3 cut(s) 416, 520, 1118
HpyAV CCTTC 3 cut(s) 489, 535, 956
HpyCH4III ACNGT 5 cut(s) 179, 351, 711, 1073, 1289
HpyCH4IV ACGT 3 cut(s) 135, 295, 424
HpyCH4V TGCA 8 cut(s) 4, 106, 388, 544, 705, 735, 852, 901
HpyF10VI GCNNNNNNNGC 3 cut(s) 253, 262, 887
HpySE526I ACGT 3 cut(s) 135, 295, 424
Hsp92II CATG 8 cut(s) 265, 386, 461, 475, 484, 656, 971, 1028
Ksp22I TGATCA 2 cut(s) 649, 727
Kzo9I GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
LguI GCTCTTC 1 cut(s) 1108
LmnI GCTCC 3 cut(s) 51, 405, 1021
LpnPI CCDG 4 cut(s) 116, 284, 559, 1092
Lsp1109I GCAGC 2 cut(s) 556, 1281
LweI GCATC 4 cut(s) 115, 736, 1103, 1239
MaeI CTAG 4 cut(s) 42, 75, 269, 276
MaeII ACGT 3 cut(s) 135, 295, 424
MaeIII GTNAC 2 cut(s) 970, 1067
MalI GATC 6 cut(s) 129, 606, 651, 729, 1012, 1111
MboI GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
MboII GAAGA 8 cut(s) 331, 381, 386, 614, 690, 945, 1095, 1225
MflI RGATCY 2 cut(s) 604, 1010
MhlI GDGCHC 1 cut(s) 1005
MlsI TGGCCA 1 cut(s) 470
MluCI AATT 9 cut(s) 11, 625, 737, 784, 798, 865, 979, 1123, 1153
MluNI TGGCCA 1 cut(s) 470
MlyI GAGTC 2 cut(s) 400, 650
MmeI TCCRAC 3 cut(s) 73, 144, 632
MnlI CCTC 8 cut(s) 73, 564, 725, 755, 1024, 1045, 1205, 1237
Mox20I TGGCCA 1 cut(s) 470
Mph1103I ATGCAT 2 cut(s) 6, 108
MscI TGGCCA 1 cut(s) 470
MseI TTAA 2 cut(s) 947, 1333
Msp20I TGGCCA 1 cut(s) 470
MspI CCGG 1 cut(s) 1325
Mva1269I GAATGC 1 cut(s) 707
MwoI GCNNNNNNNGC 3 cut(s) 253, 262, 887
NdeII GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
NlaIII CATG 8 cut(s) 265, 386, 461, 475, 484, 656, 971, 1028
NlaIV GGNNCC 3 cut(s) 47, 168, 1012
NmuCI GTSAC 2 cut(s) 970, 1067
NsiI ATGCAT 2 cut(s) 6, 108
NspI RCATGY 1 cut(s) 1028
NspV TTCGAA 2 cut(s) 840, 931
PagI TCATGA 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 1108
PcsI WCGNNNNNNNCGW 1 cut(s) 883
PctI GAATGC 1 cut(s) 707
PfeI GAWTC 6 cut(s) 92, 98, 290, 372, 616, 1145
PflMI CCANNNNNTGG 1 cut(s) 425
PkrI GCNGC 2 cut(s) 546, 1296
PleI GAGTC 2 cut(s) 399, 650
PpsI GAGTC 2 cut(s) 399, 650
PsiI TTATAA 1 cut(s) 509
PspFI CCCAGC 1 cut(s) 573
PspN4I GGNNCC 3 cut(s) 47, 168, 1012
PsuI RGATCY 2 cut(s) 604, 1010
RsaI GTAC 5 cut(s) 84, 353, 713, 836, 1215
RsaNI GTAC 5 cut(s) 83, 352, 712, 835, 1214
SapI GCTCTTC 1 cut(s) 1108
SaqAI TTAA 2 cut(s) 947, 1333
SatI GCNGC 2 cut(s) 545, 1295
Sau3AI GATC 6 cut(s) 127, 604, 649, 727, 1010, 1109
SchI GAGTC 2 cut(s) 400, 650
SduI GDGCHC 1 cut(s) 1005
SfaNI GCATC 4 cut(s) 115, 736, 1103, 1239
SfcI CTRYAG 1 cut(s) 376
SfuI TTCGAA 2 cut(s) 840, 931
Sse9I AATT 9 cut(s) 11, 625, 737, 784, 798, 865, 979, 1123, 1153
SsiI CCGC 4 cut(s) 792, 803, 1182, 1305
SspI AATATT 3 cut(s) 1207, 1265, 1277
SspMI CTAG 4 cut(s) 42, 75, 269, 276
StyI CCWWGG 3 cut(s) 41, 340, 660
TaaI ACNGT 5 cut(s) 179, 351, 711, 1073, 1289
TaiI ACGT 3 cut(s) 138, 298, 427
TaqI TCGA 9 cut(s) 15, 174, 699, 810, 840, 931, 1127, 1148, 1197
TaqII GACCGA 1 cut(s) 989
TasI AATT 9 cut(s) 11, 625, 737, 784, 798, 865, 979, 1123, 1153
TatI WGTACW 3 cut(s) 351, 834, 1213
TfiI GAWTC 6 cut(s) 92, 98, 290, 372, 616, 1145
Tru1I TTAA 2 cut(s) 947, 1333
Tru9I TTAA 2 cut(s) 947, 1333
TseFI GTSAC 2 cut(s) 970, 1067
TseI GCWGC 2 cut(s) 544, 1294
Tsp45I GTSAC 2 cut(s) 970, 1067
TspDTI ATGAA 2 cut(s) 303, 658
Van91I CCANNNNNTGG 1 cut(s) 425
XapI RAATTY 6 cut(s) 11, 737, 865, 979, 1123, 1153
XceI RCATGY 1 cut(s) 1028
XcmI CCANNNNNNNNNTGG 3 cut(s) 478, 1244, 1308
XmaJI CCTAGG 1 cut(s) 41
XspI CTAG 4 cut(s) 42, 75, 269, 276
Zsp2I ATGCAT 2 cut(s) 6, 108
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.