Rroxscaffold_4G00281530

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0013721
Physical Location & Seq
Reverse (-)
3802842 .. 3804086
1245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00281530.1

Sequence Viewer

Length: 1245 bp
ATGCATCATCAATGGGAATGTCTTGATCTGGACGTGGTGTTTTTGGTTCTTGATAAGTTGTTGGAACCCATCGACCGTGCTAGTTTCGGTGTGGTTTGCAAAGAATGGGGTCGTCTTGCAAGAGCCTATAAGCGATGGTGTAAGCGAAAGGTGCTTCCCATGCTCCTTATCCCTAGCAACTACGATGGATCACGTCAGCAATGGCGTTATGGAAAGAAGAGAAGACGACGACGTCGACCAAAGCACCAGGGACTACTGTACAGCATTTTGGAACGAAGAATCTACAGCCATGTGCAGAGTATTATGCTCCCATCAGTGACAGTGAGTAAACAAAGACATGGATGTAGCAGATACTATCGTGGATGTGGTCATGGTTGGGTGGCCATGATAGACATGAATGAGTTTAGAAGGGAAGCGATTATAACACTCGTCAACCCCTTCACAACAGCAATGTTGCAGTCTTTCCCTCCTTTGAAAGTCCATGGCTGGGGTATCCCAAAAGTTATCTTGTCCGAAGATCCGACTTTGAATGATCAAGACAATTATGTGGTTGTAGCTTTCTCTGATAGTGACTGCTTGGATTCCATCAAAGGAAGACAAGATTGGTATCATATCGAATTCAAACCGTACCTCGCAGAGTTTGATCTTTGCCAATTTACTGATGCTATATTTTACAGAGGCCAAATCTATATAGCTGATTTGGGAATAATTGCGGTGTCCGATTACAAAAGCAAACAACAAATGTATTTGAAAAAGTTATGCACAGTACCATTCCAAAATTTAACACAACAAGCTGAACGAGCATATCTTGCGGAATCAATCAAGGGAGACTTGTTGTATATTCAAAGATTTACAACATTTAAGAGTTCAGACGAAGGCCAGGTGACCGAGAATTTTAGGGTATATAAGATGGTGCTCAATGGATCCATTATGGAGTATGTTGAGGTAAAGAGCATTGGAGATGAGGCTTTGTTTGTCGGTGACAGTCATTCAGTATCTTTTTTGGCTTCAAGCTCTTCAGGGTGTCAACCAAATTCGATTTACTTCACAGATGATTCGATAAATTTTTGCCTATATTTAGATAACTACGGCGGACGACCAGTCCTCGACAAGAATATTGAAGTACAAGTTGATATGGGCATCTTCCATCTAGAGGATGGAACTATTAGCCAACATTACCCAAAATATTCCTTTGACGGTACGCTGCTACCACCCGCTTTTTGGATTATACCACCGGCCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295

Protein Analysis

414

Amino Acids

47.91

Weight (kDa)

8.21

Isoelectric Point (pI)

50.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 113 - 359 1.8e-40 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 422
AasI GACNNNNNNGTC 1 cut(s) 231
AatII GACGTC 1 cut(s) 235
AccI GTMKAC 1 cut(s) 235
AciI CCGC 4 cut(s) 713, 812, 1092, 1215
AclWI GGATC 4 cut(s) 196, 512, 918, 931
AcoI YGGCCR 2 cut(s) 381, 1236
AcsI RAATTY 5 cut(s) 617, 778, 892, 1033, 1063
AcuI CTGAAG 1 cut(s) 1002
AcyI GRCGYC 1 cut(s) 232
AfaI GTAC 5 cut(s) 260, 629, 768, 1125, 1201
AfiI CCNNNNNNNGG 3 cut(s) 487, 1153, 1221
AgsI TTSAA 7 cut(s) 475, 529, 622, 751, 845, 1011, 1121
AhdI GACNNNNNGTC 1 cut(s) 1100
AjiI CACGTC 2 cut(s) 34, 194
AjnI CCWGG 2 cut(s) 246, 879
AjuI GAANNNNNNNTTGG 2 cut(s) 586, 618
AluBI AGCT 4 cut(s) 557, 695, 794, 1014
AluI AGCT 4 cut(s) 557, 695, 794, 1014
Alw21I GWGCWC 1 cut(s) 918
Alw26I GTCTC 1 cut(s) 822
AlwI GGATC 4 cut(s) 196, 512, 918, 931
AoxI GGCC 4 cut(s) 381, 679, 877, 1236
ApeKI GCWGC 1 cut(s) 1204
ApoI RAATTY 5 cut(s) 617, 778, 892, 1033, 1063
AsuHPI GGTGA 2 cut(s) 895, 992
BaeI ACNNNNGTAYC 2 cut(s) 343, 376
BalI TGGCCA 1 cut(s) 383
BamHI GGATCC 1 cut(s) 923
BbsI GAAGAC 2 cut(s) 229, 601
Bbv12I GWGCWC 1 cut(s) 918
BbvI GCAGC 1 cut(s) 1191
BccI CCATC 8 cut(s) 77, 129, 179, 319, 593, 904, 1151, 1155
BceAI ACGGC 2 cut(s) 1105, 1223
BciT130I CCWGG 2 cut(s) 248, 881
BciVI GTATCC 1 cut(s) 503
BclI TGATCA 1 cut(s) 532
BcoDI GTCTC 1 cut(s) 822
BfaI CTAG 3 cut(s) 81, 174, 1151
BfmI CTRYAG 1 cut(s) 283
BfuI GTATCC 1 cut(s) 503
BisI GCNGC 1 cut(s) 1205
BlsI GCNGC 1 cut(s) 1206
Bme1390I CCNGG 2 cut(s) 248, 881
BmeRI GACNNNNNGTC 1 cut(s) 1100
BmgBI CACGTC 2 cut(s) 34, 194
BmiI GGNNCC 2 cut(s) 66, 925
BmrFI CCNGG 2 cut(s) 248, 881
BmsI GCATC 3 cut(s) 13, 652, 1149
BpiI GAAGAC 2 cut(s) 229, 601
BsaBI GATNNNNATC 1 cut(s) 606
BsaHI GRCGYC 1 cut(s) 232
BsaJI CCNNGG 2 cut(s) 247, 481
Bsc4I CCNNNNNNNGG 3 cut(s) 487, 1153, 1221
Bse118I RCCGGY 1 cut(s) 1234
Bse1I ACTGG 1 cut(s) 1100
Bse3DI GCAATG 2 cut(s) 206, 456
Bse8I GATNNNNATC 1 cut(s) 606
BseBI CCWGG 2 cut(s) 248, 881
BseDI CCNNGG 2 cut(s) 247, 481
BseGI GGATG 3 cut(s) 347, 368, 1162
BseJI GATNNNNATC 1 cut(s) 606
BseLI CCNNNNNNNGG 3 cut(s) 487, 1153, 1221
BseMI GCAATG 2 cut(s) 206, 456
BseNI ACTGG 1 cut(s) 1100
BseX3I CGGCCG 1 cut(s) 1236
BseXI GCAGC 1 cut(s) 1191
BseYI CCCAGC 1 cut(s) 486
BsgI GTGCAG 1 cut(s) 314
Bsh1285I CGRYCG 2 cut(s) 76, 1239
BshFI GGCC 4 cut(s) 383, 681, 879, 1238
BsiEI CGRYCG 2 cut(s) 76, 1239
BsiHKAI GWGCWC 1 cut(s) 918
BsiSI CCGG 1 cut(s) 1235
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 3 cut(s) 487, 1153, 1221
BsmAI GTCTC 1 cut(s) 822
BsmFI GGGAC 1 cut(s) 264
BsnI GGCC 4 cut(s) 383, 681, 879, 1238
Bsp1286I GDGCHC 1 cut(s) 918
Bsp1407I TGTACA 1 cut(s) 258
Bsp143I GATC 6 cut(s) 25, 188, 517, 532, 643, 923
Bsp19I CCATGG 1 cut(s) 481
BspACI CCGC 4 cut(s) 713, 812, 1092, 1215
BspANI GGCC 4 cut(s) 383, 681, 879, 1238
BspLI GGNNCC 2 cut(s) 66, 925
BspPI GGATC 4 cut(s) 196, 512, 918, 931
BspQI GCTCTTC 1 cut(s) 1021
BsrDI GCAATG 2 cut(s) 206, 456
BsrFI RCCGGY 1 cut(s) 1234
BsrGI TGTACA 1 cut(s) 258
BsrI ACTGG 1 cut(s) 1100
BssAI RCCGGY 1 cut(s) 1234
BssECI CCNNGG 2 cut(s) 247, 481
BssMI GATC 6 cut(s) 25, 188, 517, 532, 643, 923
BssNI GRCGYC 1 cut(s) 232
BssT1I CCWWGG 1 cut(s) 481
Bst2UI CCWGG 2 cut(s) 248, 881
Bst4CI ACNGT 7 cut(s) 77, 258, 322, 627, 766, 986, 1199
Bst6I CTCTTC 2 cut(s) 212, 1021
BstACI GRCGYC 1 cut(s) 232
BstAPI GCANNNNNTGC 1 cut(s) 809
BstAUI TGTACA 1 cut(s) 258
BstDSI CCRYGG 1 cut(s) 481
BstEII GGTNACC 1 cut(s) 883
BstF5I GGATG 3 cut(s) 347, 368, 1162
BstKTI GATC 6 cut(s) 28, 191, 520, 535, 646, 926
BstMAI GTCTC 1 cut(s) 822
BstMBI GATC 6 cut(s) 25, 188, 517, 532, 643, 923
BstMCI CGRYCG 2 cut(s) 76, 1239
BstMWI GCNNNNNNNGC 4 cut(s) 151, 160, 800, 809
BstNI CCWGG 2 cut(s) 248, 881
BstPI GGTNACC 1 cut(s) 883
BstSCI CCNGG 2 cut(s) 246, 879
BstSFI CTRYAG 1 cut(s) 283
BstV1I GCAGC 1 cut(s) 1191
BstV2I GAAGAC 2 cut(s) 229, 601
BstX2I RGATCY 2 cut(s) 517, 923
BstYI RGATCY 2 cut(s) 517, 923
BstZI CGGCCG 1 cut(s) 1236
BsuI GTATCC 1 cut(s) 503
BsuRI GGCC 4 cut(s) 383, 681, 879, 1238
BtgI CCRYGG 1 cut(s) 481
BtgZI GCGATG 1 cut(s) 148
BtrI CACGTC 2 cut(s) 34, 194
BtsCI GGATG 3 cut(s) 347, 368, 1162
BtsIMutI CAGTG 2 cut(s) 321, 327
Cfr10I RCCGGY 1 cut(s) 1234
Csp6I GTAC 5 cut(s) 259, 628, 767, 1124, 1200
CviAII CATG 7 cut(s) 160, 290, 338, 371, 385, 394, 482
CviQI GTAC 5 cut(s) 259, 628, 767, 1124, 1200
DpnI GATC 6 cut(s) 27, 190, 519, 534, 645, 925
DpnII GATC 6 cut(s) 25, 188, 517, 532, 643, 923
DrdI GACNNNNNNGTC 1 cut(s) 231
DriI GACNNNNNGTC 1 cut(s) 1100
DseDI GACNNNNNNGTC 1 cut(s) 231
EaeI YGGCCR 2 cut(s) 381, 1236
EagI CGGCCG 1 cut(s) 1236
Eam1104I CTCTTC 2 cut(s) 212, 1021
Eam1105I GACNNNNNGTC 1 cut(s) 1100
EarI CTCTTC 2 cut(s) 212, 1021
EciI GGCGGA 1 cut(s) 1107
EclXI CGGCCG 1 cut(s) 1236
Eco130I CCWWGG 1 cut(s) 481
Eco52I CGGCCG 1 cut(s) 1236
Eco57I CTGAAG 1 cut(s) 1002
Eco91I GGTNACC 1 cut(s) 883
EcoO65I GGTNACC 1 cut(s) 883
EcoRI GAATTC 1 cut(s) 617
EcoRII CCWGG 2 cut(s) 246, 879
EcoT14I CCWWGG 1 cut(s) 481
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 481
FaeI CATG 7 cut(s) 163, 293, 341, 374, 388, 397, 485
FalI AAGNNNNNCTT 2 cut(s) 815, 847
FaqI GGGAC 1 cut(s) 264
FatI CATG 7 cut(s) 159, 289, 337, 370, 384, 393, 481
FauI CCCGC 1 cut(s) 1222
FbaI TGATCA 1 cut(s) 532
FblI GTMKAC 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 1205
FokI GGATG 3 cut(s) 354, 375, 1169
Fsp4HI GCNGC 1 cut(s) 1205
FspBI CTAG 3 cut(s) 81, 174, 1151
GluI GCNGC 1 cut(s) 1205
GsaI CCCAGC 1 cut(s) 490
HaeIII GGCC 4 cut(s) 383, 681, 879, 1238
HapII CCGG 1 cut(s) 1235
Hin1I GRCGYC 1 cut(s) 232
Hin1II CATG 7 cut(s) 163, 293, 341, 374, 388, 397, 485
HincII GTYRAC 3 cut(s) 236, 433, 1028
HindII GTYRAC 3 cut(s) 236, 433, 1028
HinfI GANTC 4 cut(s) 279, 581, 815, 1055
HpaII CCGG 1 cut(s) 1235
HphI GGTGA 2 cut(s) 895, 992
Hpy166II GTNNAC 4 cut(s) 236, 329, 433, 1028
Hpy188I TCNGA 5 cut(s) 514, 522, 565, 721, 871
Hpy188III TCNNGA 5 cut(s) 23, 29, 50, 536, 1151
Hpy8I GTNNAC 4 cut(s) 236, 329, 433, 1028
Hpy99I CGWCG 3 cut(s) 231, 234, 237
HpyAV CCTTC 3 cut(s) 402, 448, 869
HpyCH4III ACNGT 7 cut(s) 77, 258, 322, 627, 766, 986, 1199
HpyCH4IV ACGT 3 cut(s) 33, 193, 232
HpyCH4V TGCA 6 cut(s) 4, 99, 119, 295, 457, 762
HpyF10VI GCNNNNNNNGC 4 cut(s) 151, 160, 800, 809
HpySE526I ACGT 3 cut(s) 33, 193, 232
Hsp92I GRCGYC 1 cut(s) 232
Hsp92II CATG 7 cut(s) 163, 293, 341, 374, 388, 397, 485
Ksp22I TGATCA 1 cut(s) 532
Kzo9I GATC 6 cut(s) 25, 188, 517, 532, 643, 923
LguI GCTCTTC 1 cut(s) 1021
LmnI GCTCC 2 cut(s) 168, 312
LpnPI CCDG 8 cut(s) 14, 233, 260, 472, 866, 893, 1005, 1113
Lsp1109I GCAGC 1 cut(s) 1191
LweI GCATC 3 cut(s) 13, 652, 1149
MaeI CTAG 3 cut(s) 81, 174, 1151
MaeII ACGT 3 cut(s) 33, 193, 232
MaeIII GTNAC 4 cut(s) 316, 569, 883, 980
MalI GATC 6 cut(s) 27, 190, 519, 534, 645, 925
MboI GATC 6 cut(s) 25, 188, 517, 532, 643, 923
MboII GAAGA 7 cut(s) 229, 234, 288, 527, 606, 1008, 1135
MflI RGATCY 2 cut(s) 517, 923
MhlI GDGCHC 1 cut(s) 918
MlsI TGGCCA 1 cut(s) 383
MluCI AATT 8 cut(s) 541, 617, 653, 708, 778, 892, 1033, 1063
MluNI TGGCCA 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 42, 545
MnlI CCTC 7 cut(s) 477, 641, 671, 937, 958, 1115, 1147
Mox20I TGGCCA 1 cut(s) 383
Mph1103I ATGCAT 1 cut(s) 6
MscI TGGCCA 1 cut(s) 383
MseI TTAA 3 cut(s) 782, 861, 1243
Msp20I TGGCCA 1 cut(s) 383
MspI CCGG 1 cut(s) 1235
MspR9I CCNGG 2 cut(s) 248, 881
MvaI CCWGG 2 cut(s) 248, 881
MwoI GCNNNNNNNGC 4 cut(s) 151, 160, 800, 809
NcoI CCATGG 1 cut(s) 481
NdeII GATC 6 cut(s) 25, 188, 517, 532, 643, 923
NlaIII CATG 7 cut(s) 163, 293, 341, 374, 388, 397, 485
NlaIV GGNNCC 2 cut(s) 66, 925
NmuCI GTSAC 4 cut(s) 316, 569, 883, 980
NsiI ATGCAT 1 cut(s) 6
PciSI GCTCTTC 1 cut(s) 1021
PcsI WCGNNNNNNNCGW 1 cut(s) 232
PfeI GAWTC 4 cut(s) 279, 581, 815, 1055
PflFI GACNNNGTC 1 cut(s) 231
PkrI GCNGC 1 cut(s) 1206
PsiI TTATAA 1 cut(s) 422
Psp6I CCWGG 2 cut(s) 246, 879
PspEI GGTNACC 1 cut(s) 883
PspFI CCCAGC 1 cut(s) 486
PspGI CCWGG 2 cut(s) 246, 879
PspN4I GGNNCC 2 cut(s) 66, 925
PsuI RGATCY 2 cut(s) 517, 923
PsyI GACNNNGTC 1 cut(s) 231
RsaI GTAC 5 cut(s) 260, 629, 768, 1125, 1201
RsaNI GTAC 5 cut(s) 259, 628, 767, 1124, 1200
SalI GTCGAC 1 cut(s) 234
SapI GCTCTTC 1 cut(s) 1021
SaqAI TTAA 3 cut(s) 782, 861, 1243
SatI GCNGC 1 cut(s) 1205
Sau3AI GATC 6 cut(s) 25, 188, 517, 532, 643, 923
ScrFI CCNGG 2 cut(s) 248, 881
SduI GDGCHC 1 cut(s) 918
SfaNI GCATC 3 cut(s) 13, 652, 1149
SfcI CTRYAG 1 cut(s) 283
Sse9I AATT 8 cut(s) 541, 617, 653, 708, 778, 892, 1033, 1063
SsiI CCGC 4 cut(s) 713, 812, 1092, 1215
SspI AATATT 2 cut(s) 1117, 1187
SspMI CTAG 3 cut(s) 81, 174, 1151
StyD4I CCNGG 2 cut(s) 246, 879
StyI CCWWGG 1 cut(s) 481
TaaI ACNGT 7 cut(s) 77, 258, 322, 627, 766, 986, 1199
TaiI ACGT 3 cut(s) 36, 196, 235
TaqI TCGA 6 cut(s) 72, 235, 615, 1037, 1058, 1107
TaqII GACCGA 1 cut(s) 902
TasI AATT 8 cut(s) 541, 617, 653, 708, 778, 892, 1033, 1063
TatI WGTACW 2 cut(s) 258, 1123
TfiI GAWTC 4 cut(s) 279, 581, 815, 1055
Tru1I TTAA 3 cut(s) 782, 861, 1243
Tru9I TTAA 3 cut(s) 782, 861, 1243
TscAI CASTG 2 cut(s) 321, 327
TseFI GTSAC 4 cut(s) 316, 569, 883, 980
TseI GCWGC 1 cut(s) 1204
Tsp45I GTSAC 4 cut(s) 316, 569, 883, 980
TspDTI ATGAA 1 cut(s) 410
TspRI CASTG 2 cut(s) 321, 327
Tth111I GACNNNGTC 1 cut(s) 231
XapI RAATTY 5 cut(s) 617, 778, 892, 1033, 1063
XbaI TCTAGA 1 cut(s) 1150
XcmI CCANNNNNNNNNTGG 2 cut(s) 1154, 1218
XmiI GTMKAC 1 cut(s) 235
XspI CTAG 3 cut(s) 81, 174, 1151
ZraI GACGTC 1 cut(s) 233
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.