FvH4_7g32420

Rhodanese-like domain-containing protein 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0008416
Physical Location & Seq
Forward (+)
23302538 .. 23304637
2100 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g32420.t1

Sequence Viewer

Length: 696 bp
ATGGCTTCTCTTACTTCAATCAGCCTACACTCAACTTCACCATTAGGATTTGCTTCTAAACATGATCATAGTTCTTGCAGCTGGCTCAAAGTGAAATCAGTAAGATCCACAAGGCAAGGATTGTCATCGCAGACCGTCCGACGGGGTCTGAAAATTCGTTGTGCAGCAACAAAACAGGCCAAATCACCAGCTGAAGAGGAATGGAAGATTAAGAGGGAGCTTCTGCTACAGAAAAAGGTAAGAAGTGTGGATGCAAAAGAAGCTCTACGGCTCCAGAAAGAAAACAACTTCGTGATTCTTGATGTACGGCCTGTGAAAGAGTTTAAAGAGGCTCATCCGCCGAATGCTGTCAATGTGCAAATCTATAGGCTTATAAAGGAGTGGACAGCTTGGGATATCGCCAGGCGCGCTGCGTTTGCATTTTTCGGCATTTTTGCAGGCACAGAAGAGAACCCTGAGTTCATCCAGACTGTGGAATCAAAGATAGATAAGGGGGCAAAGATAATAGTGGCTTGTGCTGCTGGGGGTACAATGCGACCAACCCAAAATCTACCAGAAGGTCAACAGTCAAGATCACTGATAGCGGCCTACTTGCTTGTCCTCAATGGTTATACCAATGTGTTCCACTTAGAAGGCGGACTTTACTCTTGGTTCAAAGAGGGACTGCCAGTAGAATCAGAAGACGAAGAAGAGTGA

Protein Analysis

232

Amino Acids

25.92

Weight (kDa)

9.2

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 85 - 219 3e-13 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AccB7I CCANNNNNTGG 1 cut(s) 472
AccII CGCG 1 cut(s) 408
AciI CCGC 3 cut(s) 338, 584, 636
AclWI GGATC 1 cut(s) 99
AcsI RAATTY 1 cut(s) 153
AcuI CTGAAG 1 cut(s) 213
AfaI GTAC 2 cut(s) 306, 529
AfiI CCNNNNNNNGG 2 cut(s) 141, 472
AgsI TTSAA 2 cut(s) 18, 655
AjnI CCWGG 1 cut(s) 401
AluBI AGCT 5 cut(s) 81, 191, 220, 263, 389
AluI AGCT 5 cut(s) 81, 191, 220, 263, 389
AlwI GGATC 1 cut(s) 99
AoxI GGCC 3 cut(s) 177, 308, 585
ApeKI GCWGC 4 cut(s) 78, 164, 410, 518
ApoI RAATTY 1 cut(s) 153
AspLEI GCGC 2 cut(s) 408, 410
AsuHPI GGTGA 2 cut(s) 30, 177
BbsI GAAGAC 1 cut(s) 687
BbvI GCAGC 4 cut(s) 90, 176, 397, 505
BceAI ACGGC 2 cut(s) 284, 323
BciT130I CCWGG 1 cut(s) 403
BclI TGATCA 1 cut(s) 64
BfmI CTRYAG 2 cut(s) 227, 364
BisI GCNGC 5 cut(s) 79, 165, 411, 519, 585
BlsI GCNGC 5 cut(s) 80, 166, 412, 520, 586
Bme1390I CCNGG 1 cut(s) 403
BmiI GGNNCC 1 cut(s) 272
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 1 cut(s) 241
BpiI GAAGAC 1 cut(s) 687
BpmI CTGGAG 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 124
Bsc4I CCNNNNNNNGG 2 cut(s) 141, 472
Bse1I ACTGG 1 cut(s) 668
Bse8I GATNNNNATC 1 cut(s) 124
BseBI CCWGG 1 cut(s) 403
BseGI GGATG 3 cut(s) 256, 334, 462
BseJI GATNNNNATC 1 cut(s) 124
BseLI CCNNNNNNNGG 2 cut(s) 141, 472
BseMII CTCAG 1 cut(s) 447
BseNI ACTGG 1 cut(s) 668
BsePI GCGCGC 1 cut(s) 406
BseXI GCAGC 4 cut(s) 90, 176, 397, 505
BseYI CCCAGC 1 cut(s) 521
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 408
BshFI GGCC 3 cut(s) 179, 310, 587
BslFI GGGAC 1 cut(s) 675
BslI CCNNNNNNNGG 2 cut(s) 141, 472
BsmFI GGGAC 1 cut(s) 675
BsmI GAATGC 1 cut(s) 349
BsnI GGCC 3 cut(s) 179, 310, 587
Bsp143I GATC 3 cut(s) 64, 104, 572
BspACI CCGC 3 cut(s) 338, 584, 636
BspANI GGCC 3 cut(s) 179, 310, 587
BspCNI CTCAG 1 cut(s) 448
BspFNI CGCG 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 272
BspPI GGATC 1 cut(s) 99
BsrI ACTGG 1 cut(s) 668
BssHII GCGCGC 1 cut(s) 406
BssMI GATC 3 cut(s) 64, 104, 572
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 3 cut(s) 136, 472, 567
Bst6I CTCTTC 3 cut(s) 189, 441, 684
BstC8I GCNNGC 3 cut(s) 83, 408, 439
BstDEI CTNAG 2 cut(s) 456, 628
BstF5I GGATG 3 cut(s) 256, 334, 462
BstFNI CGCG 1 cut(s) 408
BstHHI GCGC 2 cut(s) 408, 410
BstKTI GATC 3 cut(s) 67, 107, 575
BstMBI GATC 3 cut(s) 64, 104, 572
BstMWI GCNNNNNNNGC 4 cut(s) 260, 407, 416, 518
BstNI CCWGG 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 401
BstSFI CTRYAG 2 cut(s) 227, 364
BstUI CGCG 1 cut(s) 408
BstV1I GCAGC 4 cut(s) 90, 176, 397, 505
BstV2I GAAGAC 1 cut(s) 687
BstX2I RGATCY 1 cut(s) 104
BstYI RGATCY 1 cut(s) 104
BsuRI GGCC 3 cut(s) 179, 310, 587
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 3 cut(s) 256, 334, 462
BtsIMutI CAGTG 1 cut(s) 575
Cac8I GCNNGC 3 cut(s) 83, 408, 439
CfoI GCGC 2 cut(s) 408, 410
Csp6I GTAC 2 cut(s) 305, 528
CviAII CATG 1 cut(s) 62
CviQI GTAC 2 cut(s) 305, 528
DdeI CTNAG 2 cut(s) 456, 628
DpnI GATC 3 cut(s) 66, 106, 574
DpnII GATC 3 cut(s) 64, 104, 572
DraI TTTAAA 1 cut(s) 325
Eam1104I CTCTTC 3 cut(s) 189, 441, 684
EarI CTCTTC 3 cut(s) 189, 441, 684
EciI GGCGGA 2 cut(s) 327, 651
Eco32I GATATC 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 213
EcoRII CCWGG 1 cut(s) 401
EcoRV GATATC 1 cut(s) 397
FaeI CATG 1 cut(s) 65
FaiI YATR 5 cut(s) 63, 69, 366, 374, 612
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FaqI GGGAC 1 cut(s) 675
FatI CATG 1 cut(s) 61
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 5 cut(s) 79, 165, 411, 519, 585
FokI GGATG 3 cut(s) 263, 321, 449
Fsp4HI GCNGC 5 cut(s) 79, 165, 411, 519, 585
GlaI GCGC 2 cut(s) 407, 409
GluI GCNGC 5 cut(s) 79, 165, 411, 519, 585
GsaI CCCAGC 1 cut(s) 525
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 3 cut(s) 179, 310, 587
HhaI GCGC 2 cut(s) 408, 410
Hin1II CATG 1 cut(s) 65
Hin6I GCGC 2 cut(s) 406, 408
HinP1I GCGC 2 cut(s) 406, 408
HincII GTYRAC 1 cut(s) 563
HindII GTYRAC 1 cut(s) 563
HinfI GANTC 3 cut(s) 295, 476, 674
HphI GGTGA 2 cut(s) 30, 177
Hpy166II GTNNAC 2 cut(s) 384, 563
Hpy188I TCNGA 3 cut(s) 140, 150, 679
Hpy188III TCNNGA 5 cut(s) 274, 292, 299, 466, 570
Hpy8I GTNNAC 2 cut(s) 384, 563
Hpy99I CGWCG 1 cut(s) 144
HpyAV CCTTC 2 cut(s) 551, 626
HpyCH4III ACNGT 3 cut(s) 136, 472, 567
HpyCH4V TGCA 6 cut(s) 78, 164, 254, 358, 419, 437
HpyF10VI GCNNNNNNNGC 4 cut(s) 260, 407, 416, 518
HpyF3I CTNAG 2 cut(s) 456, 628
Hsp92II CATG 1 cut(s) 65
HspAI GCGC 2 cut(s) 406, 408
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 3 cut(s) 64, 104, 572
LmnI GCTCC 2 cut(s) 217, 276
Lsp1109I GCAGC 4 cut(s) 90, 176, 397, 505
LweI GCATC 1 cut(s) 241
MalI GATC 3 cut(s) 66, 106, 574
MboI GATC 3 cut(s) 64, 104, 572
MboII GAAGA 4 cut(s) 206, 217, 458, 692
MflI RGATCY 1 cut(s) 104
MluCI AATT 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 163
MnlI CCTC 5 cut(s) 190, 207, 322, 611, 652
MseI TTAA 2 cut(s) 210, 324
MspA1I CMGCKG 2 cut(s) 81, 191
MspR9I CCNGG 1 cut(s) 403
Mva1269I GAATGC 1 cut(s) 349
MvaI CCWGG 1 cut(s) 403
MvnI CGCG 1 cut(s) 408
MwoI GCNNNNNNNGC 4 cut(s) 260, 407, 416, 518
NdeII GATC 3 cut(s) 64, 104, 572
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 272
PauI GCGCGC 1 cut(s) 406
PctI GAATGC 1 cut(s) 349
PfeI GAWTC 3 cut(s) 295, 476, 674
PflFI GACNNNGTC 1 cut(s) 144
PflMI CCANNNNNTGG 1 cut(s) 472
PkrI GCNGC 5 cut(s) 80, 166, 412, 520, 586
PsiI TTATAA 1 cut(s) 374
Psp6I CCWGG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 521
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 1 cut(s) 272
PsuI RGATCY 1 cut(s) 104
PsyI GACNNNGTC 1 cut(s) 144
PteI GCGCGC 1 cut(s) 406
PvuII CAGCTG 2 cut(s) 81, 191
RsaI GTAC 2 cut(s) 306, 529
RsaNI GTAC 2 cut(s) 305, 528
SaqAI TTAA 2 cut(s) 210, 324
SatI GCNGC 5 cut(s) 79, 165, 411, 519, 585
Sau3AI GATC 3 cut(s) 64, 104, 572
ScrFI CCNGG 1 cut(s) 403
SetI ASST 7 cut(s) 83, 193, 222, 240, 265, 391, 562
SfaNI GCATC 1 cut(s) 241
SfcI CTRYAG 2 cut(s) 227, 364
Sse9I AATT 1 cut(s) 153
SsiI CCGC 3 cut(s) 338, 584, 636
StyD4I CCNGG 1 cut(s) 401
TaaI ACNGT 3 cut(s) 136, 472, 567
TasI AATT 1 cut(s) 153
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 3 cut(s) 295, 476, 674
Tru1I TTAA 2 cut(s) 210, 324
Tru9I TTAA 2 cut(s) 210, 324
TscAI CASTG 1 cut(s) 582
TseI GCWGC 4 cut(s) 78, 164, 410, 518
TspDTI ATGAA 1 cut(s) 451
TspRI CASTG 1 cut(s) 582
Tth111I GACNNNGTC 1 cut(s) 144
Van91I CCANNNNNTGG 1 cut(s) 472
XapI RAATTY 1 cut(s) 153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.