RLG00000026196

Rhodanese-like domain-containing protein 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0008416
Physical Location & Seq
Reverse (-)
1154849 .. 1156150
1302 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000026196

Sequence Viewer

Length: 696 bp
ATGGCTTCTCTTACTTCAATCAGCCTACACTCATCTTCACTATTGGGATTTGCTTCTAAACATGATCATACTTCTTGCTGGTTTACAGTGAGATCAGTAAGATCCACAAGGCAAGGATTGTCATCGCAGACCGTCCCACGGGGCCTGAAAATTCGAAGTGCAGCAACAAAACAAGCCAAAACACCAGCTGAAGAGGATTGGAAGATTAAGAGGGAACTTCTGCTACAGAAGAAGGTAAGAAGTGTGGATGCAAAAGAAGCTCTGCGGCTTCAGAAAGAAAACAACTTCTTGATTCTTGACGTACGGCCTGTAGCAGAGTTTAAAGAGGCTCATCCGCCGAACGCTGTCAATGTGCAAATTTACAGGCTTATAAAGGAGTGGACAGCTTGGGATATTGCTAGGCGAGCTGCATTTGCATTTTTTGGCATTTTTGCGGGCACAGAAGAGAACCCTGAGTTCATCCAAACTGTGGAATCAAAGATAGATAAGAAGGCAAAGATAATAGTGGCTTGTGCATCTGGGGGTACAATGAGACCAACCCAGAATCTACCAGAAGGTCAACAATCAAGATCACTGATAGCGGCCTACTTGCTTGTCCTCAACGGTTATACTAATGTGTTCCACTTAGAAGGCGGACTTTACTCGTGGTTTAAAGAGGGACTGCCGGTAGAATCAGAAGAAGAAGAAGAAGAGTGA

Protein Analysis

232

Amino Acids

25.99

Weight (kDa)

8.8

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 84 - 218 7.4e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 469
AciI CCGC 5 cut(s) 265, 335, 434, 581, 633
AclWI GGATC 1 cut(s) 96
AcsI RAATTY 2 cut(s) 150, 357
AcuI CTGAAG 2 cut(s) 210, 254
AfaI GTAC 2 cut(s) 303, 526
AfiI CCNNNNNNNGG 2 cut(s) 138, 469
AgsI TTSAA 1 cut(s) 18
AluBI AGCT 4 cut(s) 188, 260, 386, 407
AluI AGCT 4 cut(s) 188, 260, 386, 407
Alw26I GTCTC 1 cut(s) 526
AlwI GGATC 1 cut(s) 96
AoxI GGCC 3 cut(s) 142, 305, 582
ApeKI GCWGC 2 cut(s) 161, 407
ApoI RAATTY 2 cut(s) 150, 357
AspS9I GGNCC 1 cut(s) 142
AsuII TTCGAA 1 cut(s) 154
BaeGI GKGCMC 1 cut(s) 440
BauI CACGAG 1 cut(s) 643
BbvI GCAGC 2 cut(s) 173, 394
BceAI ACGGC 1 cut(s) 320
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 1 cut(s) 526
BfaI CTAG 1 cut(s) 399
BfmI CTRYAG 2 cut(s) 224, 309
BisI GCNGC 4 cut(s) 162, 266, 408, 582
BlsI GCNGC 4 cut(s) 163, 267, 409, 583
BmgT120I GGNCC 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 143
BmsI GCATC 2 cut(s) 238, 524
Bpu14I TTCGAA 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 121
BsaI GGTCTC 1 cut(s) 526
BsaJI CCNNGG 1 cut(s) 137
Bsc4I CCNNNNNNNGG 2 cut(s) 138, 469
Bse118I RCCGGY 1 cut(s) 664
Bse8I GATNNNNATC 1 cut(s) 121
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 3 cut(s) 253, 331, 459
BseJI GATNNNNATC 1 cut(s) 121
BseLI CCNNNNNNNGG 2 cut(s) 138, 469
BseMII CTCAG 1 cut(s) 444
BseSI GKGCMC 1 cut(s) 440
BseXI GCAGC 2 cut(s) 173, 394
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 3 cut(s) 144, 307, 584
BsiSI CCGG 1 cut(s) 665
BsiWI CGTACG 1 cut(s) 301
BslFI GGGAC 2 cut(s) 119, 672
BslI CCNNNNNNNGG 2 cut(s) 138, 469
BsmAI GTCTC 1 cut(s) 526
BsmFI GGGAC 2 cut(s) 119, 672
BsnI GGCC 3 cut(s) 144, 307, 584
Bso31I GGTCTC 1 cut(s) 526
Bsp119I TTCGAA 1 cut(s) 154
Bsp1286I GDGCHC 1 cut(s) 440
Bsp143I GATC 4 cut(s) 64, 92, 101, 569
BspACI CCGC 5 cut(s) 265, 335, 434, 581, 633
BspANI GGCC 3 cut(s) 144, 307, 584
BspCNI CTCAG 1 cut(s) 445
BspLI GGNNCC 1 cut(s) 143
BspPI GGATC 1 cut(s) 96
BspT104I TTCGAA 1 cut(s) 154
BspTNI GGTCTC 1 cut(s) 526
BsrFI RCCGGY 1 cut(s) 664
BssAI RCCGGY 1 cut(s) 664
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 4 cut(s) 64, 92, 101, 569
BssSI CACGAG 1 cut(s) 643
Bst2BI CACGAG 1 cut(s) 643
Bst4CI ACNGT 4 cut(s) 88, 133, 469, 605
Bst6I CTCTTC 3 cut(s) 186, 438, 684
BstBI TTCGAA 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 405, 436
BstDEI CTNAG 2 cut(s) 453, 625
BstDSI CCRYGG 1 cut(s) 137
BstF5I GGATG 3 cut(s) 253, 331, 459
BstKTI GATC 4 cut(s) 67, 95, 104, 572
BstMAI GTCTC 1 cut(s) 526
BstMBI GATC 4 cut(s) 64, 92, 101, 569
BstMWI GCNNNNNNNGC 3 cut(s) 257, 404, 413
BstSFI CTRYAG 2 cut(s) 224, 309
BstSLI GKGCMC 1 cut(s) 440
BstV1I GCAGC 2 cut(s) 173, 394
BstX2I RGATCY 1 cut(s) 101
BstYI RGATCY 1 cut(s) 101
BsuRI GGCC 3 cut(s) 144, 307, 584
BtgI CCRYGG 1 cut(s) 137
BtgZI GCGATG 1 cut(s) 108
BtsCI GGATG 3 cut(s) 253, 331, 459
BtsIMutI CAGTG 2 cut(s) 93, 572
Cac8I GCNNGC 2 cut(s) 405, 436
Cfr10I RCCGGY 1 cut(s) 664
Cfr13I GGNCC 1 cut(s) 142
Csp6I GTAC 2 cut(s) 302, 525
CviAII CATG 1 cut(s) 62
CviQI GTAC 2 cut(s) 302, 525
DdeI CTNAG 2 cut(s) 453, 625
DpnI GATC 4 cut(s) 66, 94, 103, 571
DpnII GATC 4 cut(s) 64, 92, 101, 569
DraI TTTAAA 2 cut(s) 322, 652
Eam1104I CTCTTC 3 cut(s) 186, 438, 684
EarI CTCTTC 3 cut(s) 186, 438, 684
EciI GGCGGA 2 cut(s) 324, 648
Eco31I GGTCTC 1 cut(s) 526
Eco57I CTGAAG 2 cut(s) 210, 254
EcoO109I RGGNCCY 1 cut(s) 142
FaeI CATG 1 cut(s) 65
FaiI YATR 4 cut(s) 63, 69, 371, 609
FalI AAGNNNNNCTT 2 cut(s) 621, 653
FaqI GGGAC 2 cut(s) 119, 672
FatI CATG 1 cut(s) 61
FauI CCCGC 1 cut(s) 427
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 4 cut(s) 162, 266, 408, 582
FokI GGATG 3 cut(s) 260, 318, 446
Fsp4HI GCNGC 4 cut(s) 162, 266, 408, 582
FspBI CTAG 1 cut(s) 399
GluI GCNGC 4 cut(s) 162, 266, 408, 582
HaeIII GGCC 3 cut(s) 144, 307, 584
HapII CCGG 1 cut(s) 665
Hin1II CATG 1 cut(s) 65
HincII GTYRAC 1 cut(s) 560
HindII GTYRAC 1 cut(s) 560
HinfI GANTC 4 cut(s) 292, 473, 544, 671
HpaII CCGG 1 cut(s) 665
Hpy166II GTNNAC 3 cut(s) 84, 381, 560
Hpy188I TCNGA 2 cut(s) 273, 676
Hpy188III TCNNGA 3 cut(s) 289, 296, 567
Hpy8I GTNNAC 3 cut(s) 84, 381, 560
HpyAV CCTTC 4 cut(s) 226, 484, 548, 623
HpyCH4III ACNGT 4 cut(s) 88, 133, 469, 605
HpyCH4IV ACGT 1 cut(s) 300
HpyCH4V TGCA 6 cut(s) 161, 251, 355, 410, 416, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 404, 413
HpyF3I CTNAG 2 cut(s) 453, 625
HpySE526I ACGT 1 cut(s) 300
Hsp92II CATG 1 cut(s) 65
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 64, 92, 101, 569
Lsp1109I GCAGC 2 cut(s) 173, 394
LweI GCATC 2 cut(s) 238, 524
MaeI CTAG 1 cut(s) 399
MaeII ACGT 1 cut(s) 300
MalI GATC 4 cut(s) 66, 94, 103, 571
MboI GATC 4 cut(s) 64, 92, 101, 569
MboII GAAGA 8 cut(s) 27, 203, 214, 241, 455, 689, 692, 695
MflI RGATCY 1 cut(s) 101
MhlI GDGCHC 1 cut(s) 440
MluCI AATT 2 cut(s) 150, 357
MnlI CCTC 5 cut(s) 187, 204, 319, 608, 649
MseI TTAA 3 cut(s) 207, 321, 651
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 1 cut(s) 665
MwoI GCNNNNNNNGC 3 cut(s) 257, 404, 413
NdeII GATC 4 cut(s) 64, 92, 101, 569
NlaIII CATG 1 cut(s) 65
NlaIV GGNNCC 1 cut(s) 143
NspV TTCGAA 1 cut(s) 154
PfeI GAWTC 4 cut(s) 292, 473, 544, 671
Pfl23II CGTACG 1 cut(s) 301
PflMI CCANNNNNTGG 1 cut(s) 469
PkrI GCNGC 4 cut(s) 163, 267, 409, 583
PsiI TTATAA 1 cut(s) 371
PspLI CGTACG 1 cut(s) 301
PspN4I GGNNCC 1 cut(s) 143
PspPI GGNCC 1 cut(s) 142
PsrI GAACNNNNNNTAC 2 cut(s) 207, 239
PsuI RGATCY 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 188
RsaI GTAC 2 cut(s) 303, 526
RsaNI GTAC 2 cut(s) 302, 525
SaqAI TTAA 3 cut(s) 207, 321, 651
SatI GCNGC 4 cut(s) 162, 266, 408, 582
Sau3AI GATC 4 cut(s) 64, 92, 101, 569
Sau96I GGNCC 1 cut(s) 142
SduI GDGCHC 1 cut(s) 440
SetI ASST 7 cut(s) 190, 237, 262, 303, 388, 409, 559
SfaNI GCATC 2 cut(s) 238, 524
SfcI CTRYAG 2 cut(s) 224, 309
SfuI TTCGAA 1 cut(s) 154
Sse9I AATT 2 cut(s) 150, 357
SsiI CCGC 5 cut(s) 265, 335, 434, 581, 633
SspMI CTAG 1 cut(s) 399
TaaI ACNGT 4 cut(s) 88, 133, 469, 605
TaiI ACGT 1 cut(s) 303
TaqI TCGA 1 cut(s) 154
TasI AATT 2 cut(s) 150, 357
TauI GCSGC 2 cut(s) 268, 584
TfiI GAWTC 4 cut(s) 292, 473, 544, 671
Tru1I TTAA 3 cut(s) 207, 321, 651
Tru9I TTAA 3 cut(s) 207, 321, 651
TscAI CASTG 2 cut(s) 93, 579
TseI GCWGC 2 cut(s) 161, 407
TspDTI ATGAA 1 cut(s) 448
TspRI CASTG 2 cut(s) 93, 579
Van91I CCANNNNNTGG 1 cut(s) 469
XapI RAATTY 2 cut(s) 150, 357
XspI CTAG 1 cut(s) 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.