Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008416

Physical Location & Seq

Genomic Coordinates Forward (+)

31627357 .. 31629021

Gene Structure 1665 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1225500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 714 bp

ATGGCTTCTCTGACTTCGATCACACTGAACCCATCTTATTCTTCCCCGTTGGGATCATTCGCTTCAAATCATTGCCAGCATAACCAGAATTCCTGCTGGCTTACAGCCGTCGGATCAGTCAGATCATCATCATCATCCACAAGGCAACGGTTTTCGTCGCAGGGCCTCAGAATCCGTAGTGCAGCAACAAAACAAGCTAAAACGCCAGCTGAAGAGGACTGGAAGACTAAGAGGGAACTTCTCCTACAGAAAAAGGTGAGGAGTGTGGATGTGAAAGAAGCTCTGAGGCTTCAAAAAGAAAACAACTTCGTGATTCTTGACGTGCGGCCTGAAGCGGAATTCAAAGAGGCTCATCCGCCGGGCGCGATTAACGTGCAAATATACAGGCTTATAAAGGAGTGGACAGCGTGGGACATTGCCCGCCGTGCTGCTTTCGCGTTTTTCGGCATCTTTGCCGGCACAGAAGAGAACCCTGACTTCATATCAAGTGTGGGATCGCAGCTAGATAAGAAGGCAAAGATAATAGTGGCTTGCGCCGCCGGCGGTACGATGAGACCGACCCAAAATCTCCCAGAAGGTCAACAGTCAAGATCACTGATAGCAGCCTACTTGCTCGTCCTCAACGGTTATACGAATGTCTTCCACCTAGAAGGCGGACTTTACGCGTGGTTCAAAGAGGGGCTGCCATCGGTTGCAGAAGAAGAAGAAGAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009526 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0009941 GO:0016020 GO:0031967 GO:0031975 GO:0031976 GO:0031984 GO:0034357 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0055035

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Rhodanese

Protein Analysis

238

Amino Acids

26.29

Weight (kDa)

8.73

Isoelectric Point (pI)

53.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Rhodanese	PF00581	91 - 225	4.1e-14	Rhodanese-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008416)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27700
fragaria_vesca	FvH4_7g32420 FvH4_7g32420
malus_domestica	MD01G1225500.v1.1 MD07G1296000.v1.1
prunus_persica	Prupe.2G315600_v2.0.a1 Prupe.2G315600_v2.0.a1 Prupe.2G315600_v2.0.a1
pyrus_communis	pycom01g23390 pycom07g26880
rosa_chinensis	RchiOBHm_Chr1g0381981
rosa_laevigata	RLG00000026196
rosa_multiflora	Rmu_co8508943.1_g000002 Rmu_sc0000110.1_g000001 Rmu_sc0009991.1_g000001
rosa_roxburghii	Rroxscaffold_4G00278290
rosa_rugosa	Rorug01G0432600
rosa_samantha	Rh1AG455900 Rh1BG413000 Rh1CG427900 Rh1DG445300
rosa_wichuraiana	Rw1G041330 Rw1G041520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	392
AccII	CGCG	3 cut(s)	365, 437, 665
AciI	CCGC	7 cut(s)	325, 335, 356, 421, 537, 543, 654
AclWI	GGATC	3 cut(s)	61, 121, 502
AcsI	RAATTY	2 cut(s)	88, 338
AcuI	CTGAAG	2 cut(s)	231, 351
AfaI	GTAC	1 cut(s)	547
AflIII	ACRYGT	1 cut(s)	663
AgsI	TTSAA	4 cut(s)	66, 293, 343, 673
AjiI	CACGTC	1 cut(s)	322
AluBI	AGCT	4 cut(s)	197, 209, 281, 502
AluI	AGCT	4 cut(s)	197, 209, 281, 502
Alw26I	GTCTC	1 cut(s)	547
AlwI	GGATC	3 cut(s)	61, 121, 502
AoxI	GGCC	2 cut(s)	163, 326
ApeKI	GCWGC	5 cut(s)	182, 428, 499, 602, 682
ApoI	RAATTY	2 cut(s)	88, 338
Asp700I	GAANNNNTTC	1 cut(s)	638
AspLEI	GCGC	2 cut(s)	365, 536
AspS9I	GGNCC	1 cut(s)	163
AsuC2I	CCSGG	1 cut(s)	360
AsuHPI	GGTGA	1 cut(s)	268
BbsI	GAAGAC	2 cut(s)	230, 631
BbvI	GCAGC	5 cut(s)	194, 415, 511, 614, 669
BccI	CCATC	2 cut(s)	40, 694
BceAI	ACGGC	2 cut(s)	92, 408
BcgI	CGANNNNNNTGC	2 cut(s)	355, 389
BcnI	CCSGG	1 cut(s)	360
BcoDI	GTCTC	1 cut(s)	547
BfaI	CTAG	2 cut(s)	503, 647
BfmI	CTRYAG	1 cut(s)	245
BisI	GCNGC	7 cut(s)	183, 326, 429, 500, 537, 603, 683
BlsI	GCNGC	7 cut(s)	184, 327, 430, 501, 538, 604, 684
Bme1390I	CCNGG	1 cut(s)	360
BmgBI	CACGTC	1 cut(s)	322
BmgT120I	GGNCC	1 cut(s)	163
BmrFI	CCNGG	1 cut(s)	360
BmsI	GCATC	1 cut(s)	456
BpiI	GAAGAC	2 cut(s)	230, 631
BpuMI	CCSGG	1 cut(s)	360
BsaBI	GATNNNNATC	1 cut(s)	127
BsaI	GGTCTC	1 cut(s)	547
Bse118I	RCCGGY	2 cut(s)	455, 539
Bse1I	ACTGG	1 cut(s)	224
Bse3DI	GCAATG	2 cut(s)	70, 414
Bse8I	GATNNNNATC	1 cut(s)	127
BseGI	GGATG	3 cut(s)	134, 274, 352
BseJI	GATNNNNATC	1 cut(s)	127
BseMI	GCAATG	2 cut(s)	70, 414
BseMII	CTCAG	2 cut(s)	181, 275
BseNI	ACTGG	1 cut(s)	224
BseRI	GAGGAG	1 cut(s)	274
BseXI	GCAGC	5 cut(s)	194, 415, 511, 614, 669
BsgI	GTGCAG	1 cut(s)	201
Bsh1236I	CGCG	3 cut(s)	365, 437, 665
BshFI	GGCC	2 cut(s)	165, 328
BsiSI	CCGG	3 cut(s)	359, 456, 540
BslFI	GGGAC	1 cut(s)	425
BsmAI	GTCTC	1 cut(s)	547
BsmFI	GGGAC	1 cut(s)	425
BsnI	GGCC	2 cut(s)	165, 328
Bso31I	GGTCTC	1 cut(s)	547
Bsp143I	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
BspACI	CCGC	7 cut(s)	325, 335, 356, 421, 537, 543, 654
BspANI	GGCC	2 cut(s)	165, 328
BspCNI	CTCAG	2 cut(s)	180, 276
BspFNI	CGCG	3 cut(s)	365, 437, 665
BspPI	GGATC	3 cut(s)	61, 121, 502
BspTNI	GGTCTC	1 cut(s)	547
BsrDI	GCAATG	2 cut(s)	70, 414
BsrFI	RCCGGY	2 cut(s)	455, 539
BsrI	ACTGG	1 cut(s)	224
BssAI	RCCGGY	2 cut(s)	455, 539
BssMI	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
Bst4CI	ACNGT	3 cut(s)	150, 585, 626
Bst6I	CTCTTC	3 cut(s)	207, 459, 702
BstC8I	GCNNGC	7 cut(s)	77, 98, 207, 421, 457, 532, 541
BstDEI	CTNAG	3 cut(s)	167, 228, 284
BstF5I	GGATG	3 cut(s)	134, 274, 352
BstFNI	CGCG	3 cut(s)	365, 437, 665
BstHHI	GCGC	2 cut(s)	365, 536
BstKTI	GATC	6 cut(s)	21, 56, 116, 125, 497, 593
BstMAI	GTCTC	1 cut(s)	547
BstMBI	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
BstMWI	GCNNNNNNNGC	4 cut(s)	425, 434, 536, 540
BstSCI	CCNGG	1 cut(s)	358
BstSFI	CTRYAG	1 cut(s)	245
BstUI	CGCG	3 cut(s)	365, 437, 665
BstV1I	GCAGC	5 cut(s)	194, 415, 511, 614, 669
BstV2I	GAAGAC	2 cut(s)	230, 631
BsuRI	GGCC	2 cut(s)	165, 328
BtrI	CACGTC	1 cut(s)	322
BtsCI	GGATG	3 cut(s)	134, 274, 352
BtsIMutI	CAGTG	2 cut(s)	23, 593
Cac8I	GCNNGC	7 cut(s)	77, 98, 207, 421, 457, 532, 541
CfoI	GCGC	2 cut(s)	365, 536
Cfr10I	RCCGGY	2 cut(s)	455, 539
Cfr13I	GGNCC	1 cut(s)	163
Csp6I	GTAC	1 cut(s)	546
CviQI	GTAC	1 cut(s)	546
DdeI	CTNAG	3 cut(s)	167, 228, 284
DpnI	GATC	6 cut(s)	20, 55, 115, 124, 496, 592
DpnII	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
Eam1104I	CTCTTC	3 cut(s)	207, 459, 702
EarI	CTCTTC	3 cut(s)	207, 459, 702
EciI	GGCGGA	2 cut(s)	345, 669
Eco31I	GGTCTC	1 cut(s)	547
Eco57I	CTGAAG	2 cut(s)	231, 351
EcoO109I	RGGNCCY	1 cut(s)	163
EcoRI	GAATTC	2 cut(s)	88, 338
FaiI	YATR	5 cut(s)	81, 382, 392, 482, 630
FalI	AAGNNNNNCTT	2 cut(s)	642, 674
FaqI	GGGAC	1 cut(s)	425
FauI	CCCGC	1 cut(s)	428
Fnu4HI	GCNGC	7 cut(s)	183, 326, 429, 500, 537, 603, 683
FokI	GGATG	3 cut(s)	121, 281, 339
Fsp4HI	GCNGC	7 cut(s)	183, 326, 429, 500, 537, 603, 683
FspBI	CTAG	2 cut(s)	503, 647
GlaI	GCGC	2 cut(s)	364, 535
GluI	GCNGC	7 cut(s)	183, 326, 429, 500, 537, 603, 683
HaeIII	GGCC	2 cut(s)	165, 328
HapII	CCGG	3 cut(s)	359, 456, 540
HhaI	GCGC	2 cut(s)	365, 536
Hin6I	GCGC	2 cut(s)	363, 534
HinP1I	GCGC	2 cut(s)	363, 534
HincII	GTYRAC	1 cut(s)	581
HindII	GTYRAC	1 cut(s)	581
HinfI	GANTC	2 cut(s)	171, 313
HpaII	CCGG	3 cut(s)	359, 456, 540
HphI	GGTGA	1 cut(s)	268
Hpy166II	GTNNAC	2 cut(s)	402, 581
Hpy188I	TCNGA	5 cut(s)	12, 113, 122, 170, 285
Hpy188III	TCNNGA	3 cut(s)	310, 317, 588
Hpy8I	GTNNAC	2 cut(s)	402, 581
Hpy99I	CGWCG	2 cut(s)	113, 160
HpyAV	CCTTC	3 cut(s)	505, 569, 644
HpyCH4III	ACNGT	3 cut(s)	150, 585, 626
HpyCH4IV	ACGT	2 cut(s)	321, 372
HpyCH4V	TGCA	3 cut(s)	182, 376, 695
HpyF10VI	GCNNNNNNNGC	4 cut(s)	425, 434, 536, 540
HpyF3I	CTNAG	3 cut(s)	167, 228, 284
HpySE526I	ACGT	2 cut(s)	321, 372
HspAI	GCGC	2 cut(s)	363, 534
KroI	GCCGGC	2 cut(s)	455, 539
KroNI	GCCGGC	2 cut(s)	457, 541
Kzo9I	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
Lsp1109I	GCAGC	5 cut(s)	194, 415, 511, 614, 669
LweI	GCATC	1 cut(s)	456
MaeI	CTAG	2 cut(s)	503, 647
MaeII	ACGT	2 cut(s)	321, 372
MalI	GATC	6 cut(s)	20, 55, 115, 124, 496, 592
MboI	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
MboII	GAAGA	7 cut(s)	33, 224, 235, 476, 631, 710, 713
MluCI	AATT	2 cut(s)	88, 338
MluI	ACGCGT	1 cut(s)	663
MmeI	TCCRAC	1 cut(s)	91
MnlI	CCTC	8 cut(s)	176, 208, 225, 252, 279, 340, 629, 670
MreI	CGCCGGCG	1 cut(s)	539
MroNI	GCCGGC	2 cut(s)	455, 539
MroXI	GAANNNNTTC	1 cut(s)	638
MseI	TTAA	1 cut(s)	369
MspA1I	CMGCKG	1 cut(s)	209
MspI	CCGG	3 cut(s)	359, 456, 540
MspR9I	CCNGG	1 cut(s)	360
MvnI	CGCG	3 cut(s)	365, 437, 665
MwoI	GCNNNNNNNGC	4 cut(s)	425, 434, 536, 540
NaeI	GCCGGC	2 cut(s)	457, 541
NciI	CCSGG	1 cut(s)	360
NdeII	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
NgoMIV	GCCGGC	2 cut(s)	455, 539
PcsI	WCGNNNNNNNCGW	1 cut(s)	554
PdiI	GCCGGC	2 cut(s)	457, 541
PdmI	GAANNNNTTC	1 cut(s)	638
PfeI	GAWTC	2 cut(s)	171, 313
PkrI	GCNGC	7 cut(s)	184, 327, 430, 501, 538, 604, 684
PsiI	TTATAA	1 cut(s)	392
PspPI	GGNCC	1 cut(s)	163
PsrI	GAACNNNNNNTAC	2 cut(s)	228, 260
PvuII	CAGCTG	1 cut(s)	209
RsaI	GTAC	1 cut(s)	547
RsaNI	GTAC	1 cut(s)	546
SaqAI	TTAA	1 cut(s)	369
SatI	GCNGC	7 cut(s)	183, 326, 429, 500, 537, 603, 683
Sau3AI	GATC	6 cut(s)	18, 53, 113, 122, 494, 590
Sau96I	GGNCC	1 cut(s)	163
ScrFI	CCNGG	1 cut(s)	360
SetI	ASST	9 cut(s)	199, 211, 258, 283, 324, 375, 504, 580, 648
SfaNI	GCATC	1 cut(s)	456
SfcI	CTRYAG	1 cut(s)	245
SgrAI	CRCCGGYG	1 cut(s)	539
Sse9I	AATT	2 cut(s)	88, 338
SsiI	CCGC	7 cut(s)	325, 335, 356, 421, 537, 543, 654
SspMI	CTAG	2 cut(s)	503, 647
StyD4I	CCNGG	1 cut(s)	358
TaaI	ACNGT	3 cut(s)	150, 585, 626
TaiI	ACGT	2 cut(s)	324, 375
TaqI	TCGA	1 cut(s)	17
TaqII	GACCGA	1 cut(s)	571
TasI	AATT	2 cut(s)	88, 338
TauI	GCSGC	2 cut(s)	328, 539
TfiI	GAWTC	2 cut(s)	171, 313
Tru1I	TTAA	1 cut(s)	369
Tru9I	TTAA	1 cut(s)	369
TscAI	CASTG	2 cut(s)	30, 600
TseI	GCWGC	5 cut(s)	182, 428, 499, 602, 682
TspDTI	ATGAA	1 cut(s)	469
TspGWI	ACGGA	1 cut(s)	164
TspRI	CASTG	2 cut(s)	30, 600
XapI	RAATTY	2 cut(s)	88, 338
XmnI	GAANNNNTTC	1 cut(s)	638
XspI	CTAG	2 cut(s)	503, 647

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.