Rw1G041520

Rhodanese-like domain-containing protein 14

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0008416
Physical Location & Seq
Forward (+)
68348667 .. 68349982
1316 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G041520.1

Sequence Viewer

Length: 705 bp
ATGGCTTCTCTTACTTCAATGAGTTCAATCAGCCTACACTCATCTTCACTATTGGGATTTGCTTCTAAACATGATCATACTTCTTGCTGGTTTACAGTGAGATCAGTAAGATCCACAAGGCAAGGATTGTCATCGCAGACTGTCCCACGGGGCCTGAAAATTCGAAGTGCAGCAACAAAACAAGCCAAAACTCCAGCTGAAGAGGATTGGAAGATTAAGAGGGAAATTCTGCTACAGAAGAAGGTAAGAAGTGTGGATGCAAAAGAAGCTCTGCGGCTTCAGAAAGAAAACAACTTCGTGATTCTTGACGTACGGCCTGTAGCAGAGTTTAAAGAGGCTCATCCGCCGAACGCTGTCAATGTGCAAATTTATAGGCTTATAAAGGAGTGGACAGCTTGGGACATTGCTAGGCGAGCCGCATTTGCATTTTTCGGCATTTTTGCAGGCACAGAAGAGAATCCTGAGTTCATTCAAACTGTGGAATCAAAGATAGATAAGAAGGCAAAAATAATAGTGGCTTGTGCATCTGGGGGTACAATGAGACCAACCCAGAATCTACCAGAAGGTCAACAATCAAGATCACTGATAGCGGCCTACTTGCTTGTCCTCAACGGTTATACCAACGTGTTCCACTTAGAAGGCGGACTTTACTCGTGGTTCAAAGAGGGACTGCCGGTAGAATCAGAAGAAGAAGAAAAGGAGTGA

Protein Analysis

234

Amino Acids

26.28

Weight (kDa)

9.16

Isoelectric Point (pI)

52.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 87 - 221 5.4e-14 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AciI CCGC 5 cut(s) 274, 344, 417, 590, 642
AclWI GGATC 1 cut(s) 105
AcsI RAATTY 3 cut(s) 159, 225, 366
AcuI CTGAAG 2 cut(s) 219, 263
AfaI GTAC 2 cut(s) 312, 535
AflIII ACRYGT 1 cut(s) 624
AgsI TTSAA 4 cut(s) 18, 27, 473, 661
AluBI AGCT 3 cut(s) 197, 269, 395
AluI AGCT 3 cut(s) 197, 269, 395
Alw26I GTCTC 1 cut(s) 535
AlwI GGATC 1 cut(s) 105
AoxI GGCC 3 cut(s) 151, 314, 591
ApeKI GCWGC 1 cut(s) 170
ApoI RAATTY 3 cut(s) 159, 225, 366
AspS9I GGNCC 1 cut(s) 151
AsuII TTCGAA 1 cut(s) 163
BauI CACGAG 1 cut(s) 652
BbvI GCAGC 1 cut(s) 182
BceAI ACGGC 1 cut(s) 329
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 535
BfaI CTAG 1 cut(s) 408
BfmI CTRYAG 2 cut(s) 233, 318
BisI GCNGC 4 cut(s) 171, 275, 417, 591
BlsI GCNGC 4 cut(s) 172, 276, 418, 592
BmgT120I GGNCC 1 cut(s) 151
BmiI GGNNCC 1 cut(s) 152
BmsI GCATC 2 cut(s) 247, 533
BpmI CTGGAG 1 cut(s) 177
Bpu14I TTCGAA 1 cut(s) 163
BsaBI GATNNNNATC 1 cut(s) 130
BsaI GGTCTC 1 cut(s) 535
BsaJI CCNNGG 1 cut(s) 146
Bse118I RCCGGY 1 cut(s) 673
Bse3DI GCAATG 1 cut(s) 402
Bse8I GATNNNNATC 1 cut(s) 130
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 262, 340
BseJI GATNNNNATC 1 cut(s) 130
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 453
BseXI GCAGC 1 cut(s) 182
BsgI GTGCAG 1 cut(s) 189
BshFI GGCC 3 cut(s) 153, 316, 593
BsiSI CCGG 1 cut(s) 674
BsiWI CGTACG 1 cut(s) 310
BslFI GGGAC 3 cut(s) 128, 413, 681
BsmAI GTCTC 1 cut(s) 535
BsmFI GGGAC 3 cut(s) 128, 413, 681
BsnI GGCC 3 cut(s) 153, 316, 593
Bso31I GGTCTC 1 cut(s) 535
Bsp119I TTCGAA 1 cut(s) 163
Bsp143I GATC 4 cut(s) 73, 101, 110, 578
BspACI CCGC 5 cut(s) 274, 344, 417, 590, 642
BspANI GGCC 3 cut(s) 153, 316, 593
BspCNI CTCAG 1 cut(s) 454
BspLI GGNNCC 1 cut(s) 152
BspPI GGATC 1 cut(s) 105
BspT104I TTCGAA 1 cut(s) 163
BspTNI GGTCTC 1 cut(s) 535
BsrDI GCAATG 1 cut(s) 402
BsrFI RCCGGY 1 cut(s) 673
BssAI RCCGGY 1 cut(s) 673
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 4 cut(s) 73, 101, 110, 578
BssSI CACGAG 1 cut(s) 652
Bst2BI CACGAG 1 cut(s) 652
Bst4CI ACNGT 4 cut(s) 97, 142, 478, 614
Bst6I CTCTTC 2 cut(s) 195, 447
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 2 cut(s) 414, 445
BstDEI CTNAG 2 cut(s) 462, 634
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 2 cut(s) 262, 340
BstKTI GATC 4 cut(s) 76, 104, 113, 581
BstMAI GTCTC 1 cut(s) 535
BstMBI GATC 4 cut(s) 73, 101, 110, 578
BstMWI GCNNNNNNNGC 3 cut(s) 266, 413, 422
BstSFI CTRYAG 2 cut(s) 233, 318
BstV1I GCAGC 1 cut(s) 182
BstX2I RGATCY 1 cut(s) 110
BstYI RGATCY 1 cut(s) 110
BsuRI GGCC 3 cut(s) 153, 316, 593
BtgI CCRYGG 1 cut(s) 146
BtgZI GCGATG 1 cut(s) 117
BtsCI GGATG 2 cut(s) 262, 340
BtsIMutI CAGTG 2 cut(s) 102, 581
Cac8I GCNNGC 2 cut(s) 414, 445
Cfr10I RCCGGY 1 cut(s) 673
Cfr13I GGNCC 1 cut(s) 151
Csp6I GTAC 2 cut(s) 311, 534
CviAII CATG 1 cut(s) 71
CviQI GTAC 2 cut(s) 311, 534
DdeI CTNAG 2 cut(s) 462, 634
DpnI GATC 4 cut(s) 75, 103, 112, 580
DpnII GATC 4 cut(s) 73, 101, 110, 578
DraI TTTAAA 1 cut(s) 331
Eam1104I CTCTTC 2 cut(s) 195, 447
EarI CTCTTC 2 cut(s) 195, 447
EciI GGCGGA 2 cut(s) 333, 657
Eco31I GGTCTC 1 cut(s) 535
Eco57I CTGAAG 2 cut(s) 219, 263
EcoO109I RGGNCCY 1 cut(s) 151
FaeI CATG 1 cut(s) 74
FaiI YATR 5 cut(s) 72, 78, 372, 380, 618
FalI AAGNNNNNCTT 2 cut(s) 630, 662
FaqI GGGAC 3 cut(s) 128, 413, 681
FatI CATG 1 cut(s) 70
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 4 cut(s) 171, 275, 417, 591
FokI GGATG 2 cut(s) 269, 327
Fsp4HI GCNGC 4 cut(s) 171, 275, 417, 591
FspBI CTAG 1 cut(s) 408
GluI GCNGC 4 cut(s) 171, 275, 417, 591
GsuI CTGGAG 1 cut(s) 177
HaeIII GGCC 3 cut(s) 153, 316, 593
HapII CCGG 1 cut(s) 674
Hin1II CATG 1 cut(s) 74
HincII GTYRAC 1 cut(s) 569
HindII GTYRAC 1 cut(s) 569
HinfI GANTC 5 cut(s) 301, 457, 482, 553, 680
HpaII CCGG 1 cut(s) 674
Hpy166II GTNNAC 3 cut(s) 93, 390, 569
Hpy188I TCNGA 2 cut(s) 282, 685
Hpy188III TCNNGA 4 cut(s) 298, 305, 461, 576
Hpy8I GTNNAC 3 cut(s) 93, 390, 569
HpyAV CCTTC 4 cut(s) 235, 493, 557, 632
HpyCH4III ACNGT 4 cut(s) 97, 142, 478, 614
HpyCH4IV ACGT 2 cut(s) 309, 624
HpyCH4V TGCA 6 cut(s) 170, 260, 364, 425, 443, 524
HpyF10VI GCNNNNNNNGC 3 cut(s) 266, 413, 422
HpyF3I CTNAG 2 cut(s) 462, 634
HpySE526I ACGT 2 cut(s) 309, 624
Hsp92II CATG 1 cut(s) 74
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 4 cut(s) 73, 101, 110, 578
Lsp1109I GCAGC 1 cut(s) 182
LweI GCATC 2 cut(s) 247, 533
MaeI CTAG 1 cut(s) 408
MaeII ACGT 2 cut(s) 309, 624
MalI GATC 4 cut(s) 75, 103, 112, 580
MboI GATC 4 cut(s) 73, 101, 110, 578
MboII GAAGA 8 cut(s) 36, 212, 223, 250, 464, 698, 701, 704
MflI RGATCY 1 cut(s) 110
MluCI AATT 3 cut(s) 159, 225, 366
MnlI CCTC 5 cut(s) 196, 213, 328, 617, 658
MseI TTAA 2 cut(s) 216, 330
MspA1I CMGCKG 1 cut(s) 197
MspI CCGG 1 cut(s) 674
MwoI GCNNNNNNNGC 3 cut(s) 266, 413, 422
NdeII GATC 4 cut(s) 73, 101, 110, 578
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 1 cut(s) 152
NspV TTCGAA 1 cut(s) 163
PfeI GAWTC 5 cut(s) 301, 457, 482, 553, 680
Pfl23II CGTACG 1 cut(s) 310
PkrI GCNGC 4 cut(s) 172, 276, 418, 592
PsiI TTATAA 1 cut(s) 380
PspLI CGTACG 1 cut(s) 310
PspN4I GGNNCC 1 cut(s) 152
PspPI GGNCC 1 cut(s) 151
PsuI RGATCY 1 cut(s) 110
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 2 cut(s) 312, 535
RsaNI GTAC 2 cut(s) 311, 534
SaqAI TTAA 2 cut(s) 216, 330
SatI GCNGC 4 cut(s) 171, 275, 417, 591
Sau3AI GATC 4 cut(s) 73, 101, 110, 578
Sau96I GGNCC 1 cut(s) 151
SetI ASST 7 cut(s) 199, 246, 271, 312, 397, 568, 627
SfaNI GCATC 2 cut(s) 247, 533
SfcI CTRYAG 2 cut(s) 233, 318
SfuI TTCGAA 1 cut(s) 163
Sse9I AATT 3 cut(s) 159, 225, 366
SsiI CCGC 5 cut(s) 274, 344, 417, 590, 642
SspMI CTAG 1 cut(s) 408
TaaI ACNGT 4 cut(s) 97, 142, 478, 614
TaiI ACGT 2 cut(s) 312, 627
TaqI TCGA 1 cut(s) 163
TasI AATT 3 cut(s) 159, 225, 366
TauI GCSGC 3 cut(s) 277, 419, 593
TfiI GAWTC 5 cut(s) 301, 457, 482, 553, 680
Tru1I TTAA 2 cut(s) 216, 330
Tru9I TTAA 2 cut(s) 216, 330
TscAI CASTG 2 cut(s) 102, 588
TseI GCWGC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 457
TspRI CASTG 2 cut(s) 102, 588
XapI RAATTY 3 cut(s) 159, 225, 366
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.