Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003759

Physical Location & Seq

Genomic Coordinates Forward (+)

3612210 .. 3625944

Gene Structure 13735 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1019700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3912 bp

ATGGCGGAGGAGAGGAACAACAAAAACAGGTGGAACTGGGAGGTCAGTGGCTTCGAGCCAAGGAAGTTGTCTTCTTCGTCGTCTTCGGCTGCGGATGCGGCGGCGTCCGGCTTCGACCATCACGACGATTACAAGTCCGATGCGCCGCTAGTTAGGCGATACTCGATCTCGGCTGCGTCGGCTTTGGCTCAGTCTGAGCTCGCCAACTCCAACTCCAACTCCAACAACCAGGTTGTGGCTTCCAAGCTCCAGAAGCTCAAGGATAAAGTTAAGCTTGCTAAAGAAGATTACTTGGAGTTGAGACAAGAAGCAAGTGAGTTGCATGAATACTCGAATGCGAAACTCGAACGAGCTACACGTTATCTTGGCGTTCTTGCGAACAAAACCCGAAAACTAGATCAGTTTGCTCTTGAAACTGAGGCCAGAATTTCTCCGCTCATCAATGAGAAGAGAAGGTTGTTCAATGACTTATTGACAGCCAAAGGAAACATAAAGGTATTTTGTCGTACAAGACCGTTATTTGAGGATGAAGGTTCCTCAATTGTTGAGTATCCTGATGATTACAATATCCGTGTCAATACTGGTGATGGTGCCCTGTCCAACCCCAAGAAGGATTTTGAACTTGACAGAGTTTATGGACCTCATGTTGGACAGGCTGAACTTTTCCATGATGTTCAACCACTGGTACAATCAGCATTGGATGGATATAATGTTTCCATATTTGCATATGGACAAACCAACTCGGGAAAGACACACACTATGGAAGGATCTAGCCATGATCGTGGTTTATATGCTCGGTCATTCGAGGAACTATTTGATTTATCCAACTCTGATTCCACTTCTACTTCAAGATTTAAATTCTCTGTTACAGTTTCCGAGCTCTACAACGAACAGATGAGGGATTTACTCCCAGAATCAGGAGATGCTCTACCAAAGATTCGAATGGGATCACCAGAATCCTTTGTAGAACTTGTGCAGGAAAAAGTTGATAACCCACTGGACTTCTCTAAAGTATTAAAAGCTGCATTTCAGAGCCGAGGAAATAATCCATCAAAGTTCAATGTTTCTCATTTGATCGTCACGATACATATATATTATAACAATTTGATCACTGGAGAAAACACCTACAGCAAGCTTTCTCTAGTTGACTTGGCTGGAAGTGAAGGATTAATTGCCGAAGATGATAGCAGTGAGCGCGTGACAGACTTGTTGCATGTCATGAAGTCACTTTCAGCGTTGGGAGATGTTTTGTCTTCTTTGACTTCAAAAAAGGATGCTATACCTTATGAAAATTCAATGCTTACCAAAGTACTTGCAGACTCACTAGGTGGAAGTTCAAAAACTCTGATGATTGTTAACGTCGTTCCAAATGCTTCGAATTTGTCGGAGACATTATCATCTCTCAATTTCTCTTCTAGAGCTAGAAATGCTGTTCTAAGCCTTGGGAATCGAGATACGATAAAGAAATGGAGAGATATTGCAAATGATGCACGTAAGGAGTTGTATGAAAAGGAAAAAGAAAGTCAAGATCTGAAACAAGAGGTTTTGGGACTTAAACACGCACTCAAGGATTCAAATGATCAGTGTGTCCTGCTCTTCAATGAAGTGCAGAAGGCATGGAAAGTATCGGATATGTTACAGTCAGATTTAAAGGCAGAGAATATGATGCTTGCAGATAAGCAGAAGATAGAAAAGGAGCAAAATGCACAGCTTAGAAATCAAGTAGCTCAGCTGTTACAGTTGGAGCAAGACCAGAGAGTGCAGCTTGAACAACGAGATTCTACAATTCAGGCCTTGCAGGCCAAAATTAAAAGCATCGAGTCCCTCCATTCCACTGAAGATCGGTCACCATTAGTCTCTGATCCAGGGTCTGGAATTCTATCAAATTCCAAGGCAATGGGGGACGGCATGGATTCTCCTCCAGTAACTAAGAAATTGGAAGAAGAGCTTAAAAAACGTGACGCATTAATAGAGAGGTTGCATGAAGAAAATGAAAAGTTATTTGATAGATTGACAGAGAAAACATCTTTGGCTGGCTCACCAAAGTTGTCAAGTCCATCATCCAAGGGGCCGCTAAATTTTCAGTCTCGGGACCTTGTGAGGAATGATAGTAGAGGACACTCGATGGATGTTGTTCCTTCATCACTGGCAGCTGCTGCAGATAAGACTGAGGGTACTGTTGCTTTGGTGAAAACAGGTGTGGAGAAAGTCAAAACAACCCCGGCAGGAGAATATCTTACGTCTGCCTTGAATGACTTTGATCCTGAACAGCATGACAGCCTTGCTGCCATTTCGGATGGAGCAAATAAGCTTTTGATGCTGGTTCTGGCTGCAGTAATTAAAGCAGGGGCTTCTAGGGAGCATGAAATACTTGCTGAAATAAGAGATGCTGTTTTCTCTTTTATTCGTAAAATGGAACCACAGAGGGTAATGGACACCATGCTTGTATCCCGTGTTAGAATTTTGTATATTAGATCATTGCTTGCTAGATCACCAGAGCTGCAGTCCATCAAGGTTTCTCCGGTTGAAAGTTTTCTAGAGAAGGTTAATACTGGACGTAGTAGAAGTTCCAGCCGGGGTAACAGCCCTGGAAGATCTCCTGTGCGCTATGTTGATGAGCATATCCAAGGCTTCAAAGTGAACCTTAAGCCAGAAAAGAAGTCGAAGTTTTCATCAGTTGTATCAAAGATACGAGGACTTGATCAGGATACTCCAAGGCAGCAGGTAACTGCTGGAAAGCTTAAGGAAATAAATGAGGAAGCAAAAAGTTTTGCAGTTGCAAACAAAGCTCTTGCGGCTCTGTTTGTGCATACACCAGCAGGTGAGCTTCAGCGCCAACTTAGATCCTGGCTTGCAGAAAATTTTGACTTTCTCTCTGTTACTGGAGAGGATGCATCAGGAGGGACAACTGGTCAGTTGGAGCTCCTTTCAACTGCAATCATGGATGGTTGGATGGCTGGACTTGGTGCTGCAGTGCCTCCTAATACAGATGCTCTTGGCCAACTTCTATCCGAGTATTCAAAGCGGGTCTACAGTTCTCAATTGCAGCACTTGAAGGATATTGCTGGTACATTGGCATCAGAAGGGGCAGAAGATACTGCACAGGTGGCAAAACTGCGATCAGCTCTCGAGTCTGTTGATCACAAAAGGAGAAAGATTTTGCAACAAATAAGAAGTGATGTAGCCTTGTTGACATTGGAAGATGGTGGTCCACCTATTCAAAATCCTTCCACTGCTGGTGAAGATGCACGATTAGCATCTCTCATTTCCCTCGATGGCATATTGAAACAAGTCAAGGATTTAATAAGACAATCGTCTGTAAGCACCTTAAGCAAGAACAAGAAGAAACTAATGCTTGCATCTCTGGACGAACTTGCTGAACGGATGCCTTCTCTTCTGGACATTGATCATCCATGTGCACAAAGACAAATTGCTGATGCTCGTCATCTCATCCAGTCTATTCCTGAAGAAGATGATCTTCAAGAGCAATCTCATGCTCGCAAACCATCTACAGATTTTGTGGGAGTTAATACTGAAACCGATGTGGCGCAGTGGAATGTCCTGCAGTTCAATACAGGCTCCACGACCCCATTTATCATCAAGTGTGGAGCAAACTCAAGTTCAGAACTGGTCATTAAAGCAGATGCCAAGATTCAAGAACCTAAAGGTGGTGAGGTTGTCAGGGTCGTCCCAAGACCATCTGTTCTGGAAAGTATGAGCTTGGAGGAGATGAAACATGTATTCTCCCAACTCCCTGAGGCTCTAAGCTTGCTTGCCTTGGCAAGGACTGCCGATGGAACACGAGCTCGGTATTCTAGGCTGTACAGGACTTTAGCCATGAAAGTTCCGTCTCTGAGGGATTTAGTCGGCGAACTTGAAAAGGGGGGAGTCCTAAAAGACGTGAGGTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0000913 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0005819 GO:0005829 GO:0005856 GO:0005871 GO:0005875 GO:0005886 GO:0006810 GO:0006928 GO:0006996 GO:0007017 GO:0007018 GO:0007049 GO:0007097 GO:0008017 GO:0008092 GO:0008150 GO:0009504 GO:0009507 GO:0009524 GO:0009526 GO:0009536 GO:0009657 GO:0009658 GO:0009902 GO:0009903 GO:0009904 GO:0009941 GO:0009987 GO:0015630 GO:0015631 GO:0016020 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0019750 GO:0022402 GO:0022607 GO:0030029 GO:0030048 GO:0030705 GO:0031022 GO:0031967 GO:0031975 GO:0032506 GO:0032991 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0046907 GO:0051179 GO:0051234 GO:0051301 GO:0051640 GO:0051641 GO:0051644 GO:0051647 GO:0051649 GO:0051656 GO:0051667 GO:0061640 GO:0071840 GO:0071944 GO:0099515 GO:1902410 GO:1903047

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Kinesin

Protein Analysis

1304

Amino Acids

143.8

Weight (kDa)

6.15

Isoelectric Point (pI)

44.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	149 - 303	1.6e-35	Microtubule binding
Kinesin	PF00225	169 - 476	1.3e-82	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003759)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10470 AT5G10470 AT5G65460 AT5G65460 AT5G65460 AT5G65460 AT5G65460 AT5G65460
fragaria_vesca	FvH4_1g19720 FvH4_1g19720
malus_domestica	MD00G1019700.v1.1 MD15G1313400.v1.1 MD15G1313600.v1.1
prunus_persica	Prupe.6G198800_v2.0.a1 Prupe.6G198800_v2.0.a1
pyrus_communis	pycom01g06640 pycom15g27690
rosa_chinensis	RchiOBHm_Chr2g0111301
rosa_laevigata	RLG00000007010 RLG00000017883
rosa_multiflora	Rmu_co8453119.1_g000001 Rmu_sc0001363.1_g000009 Rmu_sc0001363.1_g000010 Rmu_sc0036570.1_g000001 Rmu_sc0036571.1_g000001
rosa_roxburghii	Rroxscaffold_2G00132520
rosa_rugosa	Rorug02G0172700
rosa_samantha	Rh2AG225700 Rh2BG237400 Rh2CG228700 Rh2DG233200 Rh4DG010800 Rh5BG175600
rosa_wichuraiana	Rw2G017420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AarI	CACCTGC	1 cut(s)	2810
Acc36I	ACCTGC	2 cut(s)	2713, 2810
AccB1I	GGYRCC	1 cut(s)	590
AccB7I	CCANNNNNTGG	2 cut(s)	235, 1871
AccBSI	CCGCTC	1 cut(s)	436
AccI	GTMKAC	1 cut(s)	3030
AccII	CGCG	1 cut(s)	1197
AciI	CCGC	9 cut(s)	5, 92, 98, 101, 146, 434, 2072, 2795, 3025
AclWI	GGATC	5 cut(s)	775, 955, 1856, 2255, 2838
AcoI	YGGCCR	1 cut(s)	2998
AcsI	RAATTY	9 cut(s)	426, 857, 1291, 1378, 1875, 1885, 2077, 2460, 2860
AcuI	CTGAAG	3 cut(s)	1857, 2813, 3486
AcyI	GRCGYC	1 cut(s)	104
AdeI	CACNNNGTG	1 cut(s)	1328
AfaI	GTAC	6 cut(s)	508, 687, 1311, 2176, 3070, 3824
AfiI	CCNNNNNNNGG	7 cut(s)	235, 610, 647, 917, 1871, 3668, 3783
AflII	CTTAAG	3 cut(s)	2645, 2741, 3328
AflIII	ACRYGT	2 cut(s)	356, 3736
AhdI	GACNNNNNGTC	2 cut(s)	2910, 3904
AjiI	CACGTC	1 cut(s)	3901
AjnI	CCWGG	4 cut(s)	228, 1864, 2587, 2846
AjuI	GAANNNNNNNTTGG	2 cut(s)	2012, 2044
Alw21I	GWGCWC	5 cut(s)	201, 882, 2925, 3421, 3808
Alw26I	GTCTC	5 cut(s)	295, 1382, 1861, 2091, 3855
Alw44I	GTGCAC	1 cut(s)	3417
AlwI	GGATC	5 cut(s)	775, 955, 1856, 2255, 2838
AlwNI	CAGNNNCTG	2 cut(s)	1871, 2156
Ama87I	CYCGRG	3 cut(s)	742, 2088, 3128
AoxI	GGCC	5 cut(s)	420, 1791, 1800, 2069, 2998
ApaLI	GTGCAC	1 cut(s)	3417
ApoI	RAATTY	9 cut(s)	426, 857, 1291, 1378, 1875, 1885, 2077, 2460, 2860
AseI	ATTAAT	2 cut(s)	1169, 1967
Asp700I	GAANNNNTTC	1 cut(s)	2532
AspLEI	GCGC	5 cut(s)	145, 1197, 2607, 2835, 3550
AspS9I	GGNCC	4 cut(s)	638, 2069, 2092, 3209
AsuC2I	CCSGG	2 cut(s)	2222, 2576
AsuHPI	GGTGA	9 cut(s)	596, 942, 1839, 2031, 2200, 2484, 2834, 3251, 3683
AsuII	TTCGAA	2 cut(s)	940, 1376
AvaI	CYCGRG	3 cut(s)	742, 2088, 3128
AvaII	GGWCC	3 cut(s)	638, 2092, 3209
AxyI	CCTNAGG	1 cut(s)	3756
BaeGI	GKGCMC	2 cut(s)	595, 3421
BalI	TGGCCA	1 cut(s)	3000
BanI	GGYRCC	1 cut(s)	590
BanII	GRGCYC	4 cut(s)	201, 882, 2925, 3808
BauI	CACGAG	1 cut(s)	3801
BbsI	GAAGAC	3 cut(s)	63, 75, 1245
Bbv12I	GWGCWC	5 cut(s)	201, 882, 2925, 3421, 3808
BceAI	ACGGC	1 cut(s)	1921
BciT130I	CCWGG	4 cut(s)	230, 1866, 2589, 2848
BciVI	GTATCC	3 cut(s)	561, 2458, 2701
BclI	TGATCA	5 cut(s)	1107, 1579, 2701, 3139, 3406
BcnI	CCSGG	2 cut(s)	2222, 2576
BcoDI	GTCTC	5 cut(s)	295, 1382, 1861, 2091, 3855
BfmI	CTRYAG	8 cut(s)	1126, 2157, 2331, 2501, 2970, 3031, 3510, 3563
BfoI	RGCGCY	1 cut(s)	2836
BfrI	CTTAAG	3 cut(s)	2645, 2741, 3328
BfuAI	ACCTGC	2 cut(s)	2713, 2810
BfuI	GTATCC	3 cut(s)	561, 2458, 2701
BglI	GCCNNNNNGGC	1 cut(s)	1799
BglII	AGATCT	2 cut(s)	1528, 2594
BlpI	GCTNAGC	1 cut(s)	1728
BmcAI	AGTACT	1 cut(s)	1311
Bme1390I	CCNGG	6 cut(s)	230, 1866, 2222, 2576, 2589, 2848
Bme18I	GGWCC	3 cut(s)	638, 2092, 3209
BmeRI	GACNNNNNGTC	2 cut(s)	2910, 3904
BmeT110I	CYCGRG	3 cut(s)	742, 2088, 3128
BmgBI	CACGTC	1 cut(s)	3901
BmgT120I	GGNCC	4 cut(s)	638, 2069, 2092, 3209
BmiI	GGNNCC	6 cut(s)	535, 592, 2070, 2093, 2418, 3580
BmrFI	CCNGG	6 cut(s)	230, 1866, 2222, 2576, 2589, 2848
BmrI	ACTGGG	1 cut(s)	46
BmuI	ACTGGG	1 cut(s)	46
BoxI	GACNNNNGTC	1 cut(s)	3313
BpiI	GAAGAC	3 cut(s)	63, 75, 1245
BpmI	CTGGAG	4 cut(s)	233, 1134, 1905, 2904
Bpu1102I	GCTNAGC	1 cut(s)	1728
Bpu14I	TTCGAA	2 cut(s)	940, 1376
BpuEI	CTTGAG	3 cut(s)	242, 1550, 3601
BpuMI	CCSGG	2 cut(s)	2222, 2576
BsaAI	YACGTR	1 cut(s)	1493
BsaBI	GATNNNNATC	1 cut(s)	3256
BsaHI	GRCGYC	1 cut(s)	104
BsaWI	WCCGGW	1 cut(s)	2521
BsaXI	ACNNNNNCTCC	4 cut(s)	197, 203, 227, 233
Bsc4I	CCNNNNNNNGG	7 cut(s)	235, 610, 647, 917, 1871, 3668, 3783
Bse21I	CCTNAGG	1 cut(s)	3756
Bse3DI	GCAATG	2 cut(s)	1902, 2477
Bse8I	GATNNNNATC	1 cut(s)	3256
BseBI	CCWGG	4 cut(s)	230, 1866, 2589, 2848
BseJI	GATNNNNATC	1 cut(s)	3256
BseLI	CCNNNNNNNGG	7 cut(s)	235, 610, 647, 917, 1871, 3668, 3783
BseMI	GCAATG	2 cut(s)	1902, 2477
BseMII	CTCAG	7 cut(s)	186, 203, 408, 1742, 2160, 3747, 3845
BseRI	GAGGAG	3 cut(s)	23, 1908, 3740
BseSI	GKGCMC	2 cut(s)	595, 3421
BsgI	GTGCAG	4 cut(s)	995, 1628, 1781, 3084
Bsh1236I	CGCG	1 cut(s)	1197
BshFI	GGCC	5 cut(s)	422, 1793, 1802, 2071, 3000
BshNI	GGYRCC	1 cut(s)	590
BsiHKAI	GWGCWC	5 cut(s)	201, 882, 2925, 3421, 3808
BsiHKCI	CYCGRG	3 cut(s)	742, 2088, 3128
BsiSI	CCGG	4 cut(s)	108, 2222, 2522, 2575
BslFI	GGGAC	6 cut(s)	1563, 1807, 1916, 2105, 2917, 3674
BslI	CCNNNNNNNGG	7 cut(s)	235, 610, 647, 917, 1871, 3668, 3783
BsmAI	GTCTC	5 cut(s)	295, 1382, 1861, 2091, 3855
BsmBI	CGTCTC	1 cut(s)	3855
BsmFI	GGGAC	6 cut(s)	1563, 1807, 1916, 2105, 2917, 3674
BsmI	GAATGC	1 cut(s)	340
BsnI	GGCC	5 cut(s)	422, 1793, 1802, 2071, 3000
BsoBI	CYCGRG	3 cut(s)	742, 2088, 3128
Bsp119I	TTCGAA	2 cut(s)	940, 1376
Bsp1286I	GDGCHC	6 cut(s)	201, 595, 882, 2925, 3421, 3808
Bsp1407I	TGTACA	1 cut(s)	3822
Bsp1720I	GCTNAGC	1 cut(s)	1728
BspACI	CCGC	9 cut(s)	5, 92, 98, 101, 146, 434, 2072, 2795, 3025
BspANI	GGCC	5 cut(s)	422, 1793, 1802, 2071, 3000
BspCNI	CTCAG	7 cut(s)	187, 202, 409, 1741, 2161, 3748, 3846
BspFNI	CGCG	1 cut(s)	1197
BspHI	TCATGA	2 cut(s)	1218, 3908
BspLI	GGNNCC	6 cut(s)	535, 592, 2070, 2093, 2418, 3580
BspMAI	CTGCAG	5 cut(s)	2161, 2335, 2505, 2974, 3567
BspMI	ACCTGC	2 cut(s)	2713, 2810
BspPI	GGATC	5 cut(s)	775, 955, 1856, 2255, 2838
BspQI	GCTCTTC	2 cut(s)	1601, 1938
BspT104I	TTCGAA	2 cut(s)	940, 1376
BspT107I	GGYRCC	1 cut(s)	590
BspTI	CTTAAG	3 cut(s)	2645, 2741, 3328
BsrBI	CCGCTC	1 cut(s)	436
BsrDI	GCAATG	2 cut(s)	1902, 2477
BsrGI	TGTACA	1 cut(s)	3822
BssNI	GRCGYC	1 cut(s)	104
BssSI	CACGAG	1 cut(s)	3801
BssT1I	CCWWGG	7 cut(s)	59, 1441, 1890, 2064, 2626, 2714, 3777
Bst2BI	CACGAG	1 cut(s)	3801
Bst2UI	CCWGG	4 cut(s)	230, 1866, 2589, 2848
Bst4CI	ACNGT	6 cut(s)	516, 871, 1641, 1740, 2179, 3035
Bst6I	CTCTTC	5 cut(s)	443, 1417, 1601, 1938, 3399
BstACI	GRCGYC	1 cut(s)	104
BstAFI	CTTAAG	3 cut(s)	2645, 2741, 3328
BstAPI	GCANNNNNTGC	3 cut(s)	1487, 2156, 3788
BstAUI	TGTACA	1 cut(s)	3822
BstBAI	YACGTR	1 cut(s)	1493
BstBI	TTCGAA	2 cut(s)	940, 1376
BstEII	GGTNACC	1 cut(s)	1845
BstENI	CCTNNNNNAGG	1 cut(s)	3781
BstFNI	CGCG	1 cut(s)	1197
BstH2I	RGCGCY	1 cut(s)	2836
BstHHI	GCGC	5 cut(s)	145, 1197, 2607, 2835, 3550
BstMAI	GTCTC	5 cut(s)	295, 1382, 1861, 2091, 3855
BstNI	CCWGG	4 cut(s)	230, 1866, 2589, 2848
BstNSI	RCATGY	2 cut(s)	1217, 3740
BstPAI	GACNNNNGTC	1 cut(s)	3313
BstPI	GGTNACC	1 cut(s)	1845
BstSCI	CCNGG	6 cut(s)	228, 1864, 2220, 2574, 2587, 2846
BstSFI	CTRYAG	8 cut(s)	1126, 2157, 2331, 2501, 2970, 3031, 3510, 3563
BstSLI	GKGCMC	2 cut(s)	595, 3421
BstUI	CGCG	1 cut(s)	1197
BstV2I	GAAGAC	3 cut(s)	63, 75, 1245
BstX2I	RGATCY	4 cut(s)	767, 1528, 2594, 2843
BstXI	CCANNNNNNTGG	2 cut(s)	782, 1897
BstYI	RGATCY	4 cut(s)	767, 1528, 2594, 2843
Bsu36I	CCTNAGG	1 cut(s)	3756
BsuI	GTATCC	3 cut(s)	561, 2458, 2701
BsuRI	GGCC	5 cut(s)	422, 1793, 1802, 2071, 3000
BtrI	CACGTC	1 cut(s)	3901
BtsI	GCAGTG	4 cut(s)	1195, 2979, 3231, 3557
BveI	ACCTGC	2 cut(s)	2713, 2810
CaiI	CAGNNNCTG	2 cut(s)	1871, 2156
CciI	TCATGA	2 cut(s)	1218, 3908
CfoI	GCGC	5 cut(s)	145, 1197, 2607, 2835, 3550
Cfr13I	GGNCC	4 cut(s)	638, 2069, 2092, 3209
CseI	GACGC	3 cut(s)	93, 165, 1970
CsiI	ACCWGGT	1 cut(s)	228
Csp6I	GTAC	6 cut(s)	507, 686, 1310, 2175, 3069, 3823
CviQI	GTAC	6 cut(s)	507, 686, 1310, 2175, 3069, 3823
DraI	TTTAAA	2 cut(s)	856, 1650
DraIII	CACNNNGTG	1 cut(s)	1328
DriI	GACNNNNNGTC	2 cut(s)	2910, 3904
EaeI	YGGCCR	1 cut(s)	2998
Eam1104I	CTCTTC	5 cut(s)	443, 1417, 1601, 1938, 3399
Eam1105I	GACNNNNNGTC	2 cut(s)	2910, 3904
EarI	CTCTTC	5 cut(s)	443, 1417, 1601, 1938, 3399
EciI	GGCGGA	1 cut(s)	20
Ecl136II	GAGCTC	4 cut(s)	199, 880, 2923, 3806
Eco130I	CCWWGG	7 cut(s)	59, 1441, 1890, 2064, 2626, 2714, 3777
Eco147I	AGGCCT	1 cut(s)	1793
Eco24I	GRGCYC	4 cut(s)	201, 882, 2925, 3808
Eco47I	GGWCC	3 cut(s)	638, 2092, 3209
Eco53kI	GAGCTC	4 cut(s)	199, 880, 2923, 3806
Eco57I	CTGAAG	3 cut(s)	1857, 2813, 3486
Eco81I	CCTNAGG	1 cut(s)	3756
Eco88I	CYCGRG	3 cut(s)	742, 2088, 3128
Eco91I	GGTNACC	1 cut(s)	1845
EcoICRI	GAGCTC	4 cut(s)	199, 880, 2923, 3806
EcoNI	CCTNNNNNAGG	1 cut(s)	3781
EcoO109I	RGGNCCY	1 cut(s)	2092
EcoO65I	GGTNACC	1 cut(s)	1845
EcoRI	GAATTC	1 cut(s)	1875
EcoRII	CCWGG	4 cut(s)	228, 1864, 2587, 2846
EcoT14I	CCWWGG	7 cut(s)	59, 1441, 1890, 2064, 2626, 2714, 3777
EcoT22I	ATGCAT	1 cut(s)	2896
EcoT38I	GRGCYC	4 cut(s)	201, 882, 2925, 3808
ErhI	CCWWGG	7 cut(s)	59, 1441, 1890, 2064, 2626, 2714, 3777
Esp3I	CGTCTC	1 cut(s)	3855
FalI	AAGNNNNNCTT	8 cut(s)	258, 290, 1932, 1964, 2628, 2660, 3462, 3494
FaqI	GGGAC	6 cut(s)	1563, 1807, 1916, 2105, 2917, 3674
FauI	CCCGC	1 cut(s)	3018
FauNDI	CATATG	1 cut(s)	727
FbaI	TGATCA	5 cut(s)	1107, 1579, 2701, 3139, 3406
FblI	GTMKAC	1 cut(s)	3030
FriOI	GRGCYC	4 cut(s)	201, 882, 2925, 3808
GlaI	GCGC	5 cut(s)	144, 1196, 2606, 2834, 3549
GsuI	CTGGAG	4 cut(s)	233, 1134, 1905, 2904
HaeII	RGCGCY	1 cut(s)	2836
HaeIII	GGCC	5 cut(s)	422, 1793, 1802, 2071, 3000
HapII	CCGG	4 cut(s)	108, 2222, 2522, 2575
HgaI	GACGC	3 cut(s)	93, 165, 1970
HhaI	GCGC	5 cut(s)	145, 1197, 2607, 2835, 3550
Hin1I	GRCGYC	1 cut(s)	104
Hin6I	GCGC	5 cut(s)	143, 1195, 2605, 2833, 3548
HinP1I	GCGC	5 cut(s)	143, 1195, 2605, 2833, 3548
HincII	GTYRAC	3 cut(s)	1147, 1357, 3192
HindII	GTYRAC	3 cut(s)	1147, 1357, 3192
HindIII	AAGCTT	5 cut(s)	272, 1133, 2309, 2738, 3766
HpaI	GTTAAC	1 cut(s)	1357
HpaII	CCGG	4 cut(s)	108, 2222, 2522, 2575
HphI	GGTGA	9 cut(s)	596, 942, 1839, 2031, 2200, 2484, 2834, 3251, 3683
Hpy166II	GTNNAC	7 cut(s)	1147, 1357, 2641, 3031, 3192, 3212, 3419
Hpy8I	GTNNAC	7 cut(s)	1147, 1357, 2641, 3031, 3192, 3212, 3419
Hpy99I	CGWCG	4 cut(s)	82, 128, 181, 1364
HpyCH4III	ACNGT	6 cut(s)	516, 871, 1641, 1740, 2179, 3035
HpyCH4IV	ACGT	7 cut(s)	358, 1359, 1492, 1957, 2240, 2557, 3900
HpySE526I	ACGT	7 cut(s)	358, 1359, 1492, 1957, 2240, 2557, 3900
Hsp92I	GRCGYC	1 cut(s)	104
HspAI	GCGC	5 cut(s)	143, 1195, 2605, 2833, 3548
Ksp22I	TGATCA	5 cut(s)	1107, 1579, 2701, 3139, 3406
KspAI	GTTAAC	1 cut(s)	1357
LguI	GCTCTTC	2 cut(s)	1601, 1938
LmnI	GCTCC	9 cut(s)	252, 1696, 1744, 2300, 2359, 2920, 2928, 3584, 3608
MabI	ACCWGGT	1 cut(s)	228
MaeII	ACGT	7 cut(s)	358, 1359, 1492, 1957, 2240, 2557, 3900
MbiI	CCGCTC	1 cut(s)	436
MfeI	CAATTG	2 cut(s)	540, 3041
MflI	RGATCY	4 cut(s)	767, 1528, 2594, 2843
MhlI	GDGCHC	6 cut(s)	201, 595, 882, 2925, 3421, 3808
MlsI	TGGCCA	1 cut(s)	3000
MluNI	TGGCCA	1 cut(s)	3000
MlyI	GAGTC	4 cut(s)	1313, 1829, 3140, 3897
Mox20I	TGGCCA	1 cut(s)	3000
Mph1103I	ATGCAT	1 cut(s)	2896
MroXI	GAANNNNTTC	1 cut(s)	2532
MscI	TGGCCA	1 cut(s)	3000
MslI	CAYNNNNRTG	2 cut(s)	780, 3414
Msp20I	TGGCCA	1 cut(s)	3000
MspA1I	CMGCKG	2 cut(s)	1732, 2153
MspCI	CTTAAG	3 cut(s)	2645, 2741, 3328
MspI	CCGG	4 cut(s)	108, 2222, 2522, 2575
MspR9I	CCNGG	6 cut(s)	230, 1866, 2222, 2576, 2589, 2848
MunI	CAATTG	2 cut(s)	540, 3041
Mva1269I	GAATGC	1 cut(s)	340
MvaI	CCWGG	4 cut(s)	230, 1866, 2589, 2848
MvnI	CGCG	1 cut(s)	1197
NciI	CCSGG	2 cut(s)	2222, 2576
NdeI	CATATG	1 cut(s)	727
NlaIV	GGNNCC	6 cut(s)	535, 592, 2070, 2093, 2418, 3580
NmeAIII	GCCGAG	2 cut(s)	149, 1061
NmuCI	GTSAC	5 cut(s)	1078, 1198, 1224, 1845, 1958
NsiI	ATGCAT	1 cut(s)	2896
NspI	RCATGY	2 cut(s)	1217, 3740
NspV	TTCGAA	2 cut(s)	940, 1376
PaeR7I	CTCGAG	1 cut(s)	3128
PagI	TCATGA	2 cut(s)	1218, 3908
PaqCI	CACCTGC	1 cut(s)	2810
PceI	AGGCCT	1 cut(s)	1793
PciI	ACATGT	1 cut(s)	3736
PciSI	GCTCTTC	2 cut(s)	1601, 1938
PcsI	WCGNNNNNNNCGW	2 cut(s)	120, 355
PctI	GAATGC	1 cut(s)	340
PdmI	GAANNNNTTC	1 cut(s)	2532
PfeI	GAWTC	9 cut(s)	833, 914, 937, 956, 1447, 1571, 1778, 1913, 3652
PflMI	CCANNNNNTGG	2 cut(s)	235, 1871
PleI	GAGTC	4 cut(s)	1313, 1828, 3139, 3896
PpsI	GAGTC	4 cut(s)	1313, 1828, 3139, 3896
Ppu21I	YACGTR	1 cut(s)	1493
PpuMI	RGGWCCY	1 cut(s)	2092
PscI	ACATGT	1 cut(s)	3736
PshAI	GACNNNNGTC	1 cut(s)	3313
PshBI	ATTAAT	2 cut(s)	1169, 1967
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	4 cut(s)	201, 882, 2925, 3808
Psp5II	RGGWCCY	1 cut(s)	2092
Psp6I	CCWGG	4 cut(s)	228, 1864, 2587, 2846
PspEI	GGTNACC	1 cut(s)	1845
PspGI	CCWGG	4 cut(s)	228, 1864, 2587, 2846
PspN4I	GGNNCC	6 cut(s)	535, 592, 2070, 2093, 2418, 3580
PspPI	GGNCC	4 cut(s)	638, 2069, 2092, 3209
PspPPI	RGGWCCY	1 cut(s)	2092
PsrI	GAACNNNNNNTAC	2 cut(s)	2551, 2583
PstI	CTGCAG	5 cut(s)	2161, 2335, 2505, 2974, 3567
PstNI	CAGNNNCTG	2 cut(s)	1871, 2156
PsuI	RGATCY	4 cut(s)	767, 1528, 2594, 2843
PvuII	CAGCTG	2 cut(s)	1732, 2153
RsaI	GTAC	6 cut(s)	508, 687, 1311, 2176, 3070, 3824
RsaNI	GTAC	6 cut(s)	507, 686, 1310, 2175, 3069, 3823
RseI	CAYNNNNRTG	2 cut(s)	780, 3414
SacI	GAGCTC	4 cut(s)	201, 882, 2925, 3808
SapI	GCTCTTC	2 cut(s)	1601, 1938
Sau96I	GGNCC	4 cut(s)	638, 2069, 2092, 3209
ScaI	AGTACT	1 cut(s)	1311
SchI	GAGTC	4 cut(s)	1313, 1829, 3140, 3897
ScrFI	CCNGG	6 cut(s)	230, 1866, 2222, 2576, 2589, 2848
SduI	GDGCHC	6 cut(s)	201, 595, 882, 2925, 3421, 3808
SexAI	ACCWGGT	1 cut(s)	228
SfcI	CTRYAG	8 cut(s)	1126, 2157, 2331, 2501, 2970, 3031, 3510, 3563
SfiI	GGCCNNNNNGGCC	1 cut(s)	1799
Sfr274I	CTCGAG	1 cut(s)	3128
SfuI	TTCGAA	2 cut(s)	940, 1376
SinI	GGWCC	3 cut(s)	638, 2092, 3209
SlaI	CTCGAG	1 cut(s)	3128
SmiI	ATTTAAAT	1 cut(s)	856
SmiMI	CAYNNNNRTG	2 cut(s)	780, 3414
SmlI	CTYRAG	7 cut(s)	257, 1565, 2645, 2741, 3128, 3328, 3616
SmoI	CTYRAG	7 cut(s)	257, 1565, 2645, 2741, 3128, 3328, 3616
SseBI	AGGCCT	1 cut(s)	1793
SsiI	CCGC	9 cut(s)	5, 92, 98, 101, 146, 434, 2072, 2795, 3025
SstI	GAGCTC	4 cut(s)	201, 882, 2925, 3808
StuI	AGGCCT	1 cut(s)	1793
StyD4I	CCNGG	6 cut(s)	228, 1864, 2220, 2574, 2587, 2846
StyI	CCWWGG	7 cut(s)	59, 1441, 1890, 2064, 2626, 2714, 3777
SwaI	ATTTAAAT	1 cut(s)	856
TaaI	ACNGT	6 cut(s)	516, 871, 1641, 1740, 2179, 3035
TaiI	ACGT	7 cut(s)	361, 1362, 1495, 1960, 2243, 2560, 3903
TaqII	GACCGA	2 cut(s)	786, 1833
TatI	WGTACW	2 cut(s)	1309, 3822
TauI	GCSGC	5 cut(s)	101, 104, 148, 2074, 2798
TfiI	GAWTC	9 cut(s)	833, 914, 937, 956, 1447, 1571, 1778, 1913, 3652
TseFI	GTSAC	5 cut(s)	1078, 1198, 1224, 1845, 1958
Tsp45I	GTSAC	5 cut(s)	1078, 1198, 1224, 1845, 1958
TspGWI	ACGGA	3 cut(s)	560, 3397, 3837
Van91I	CCANNNNNTGG	2 cut(s)	235, 1871
Vha464I	CTTAAG	3 cut(s)	2645, 2741, 3328
VneI	GTGCAC	1 cut(s)	3417
VpaK11BI	GGWCC	3 cut(s)	638, 2092, 3209
VspI	ATTAAT	2 cut(s)	1169, 1967
XagI	CCTNNNNNAGG	1 cut(s)	3781
XapI	RAATTY	9 cut(s)	426, 857, 1291, 1378, 1875, 1885, 2077, 2460, 2860
XbaI	TCTAGA	2 cut(s)	1415, 2536
XceI	RCATGY	2 cut(s)	1217, 3740
XhoI	CTCGAG	1 cut(s)	3128
XmiI	GTMKAC	1 cut(s)	3030
XmnI	GAANNNNTTC	1 cut(s)	2532
ZrmI	AGTACT	1 cut(s)	1311
Zsp2I	ATGCAT	1 cut(s)	2896

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.