MD00G1031100.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0005708
Physical Location & Seq
Forward (+)
5158760 .. 5185116
26357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1031100.v1.1.491

Sequence Viewer

Length: 7314 bp
ATGGCGGCCTCGGGGCAGTCCCTCAGGTTCTGCGGCCCCGGCTCCGCCGGACCCAGTGGAGGAAGTTTTGACGCGCTCAATCGCATCCTTGCTGACCTCTGCACCCGTGGCAGCCCCAAGGATGGGGCTTCGTTAGCATTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACCTCAGCGGAGAAGCATTTTCGCGTTTCATGGATCAGTTGTATGATCGAATTTCATCTCTACTGGAGAGCAGTGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGATGAGTTGATAGATGTGGCACTGGGAGAGAATTCCTCCAAGGTTTCGAAGTTTGCTAACTACATACGGACTGTTTTTGAGGTAAAGCGTGATCCGGATATTCTGGTGCTTGCTAGTAGAGTTCTGGGCCATCTTGCTAGGGCAGGGGGAGCAATGACTGCAGATGAAGTGGAACGCCAGATAAAAATTGCTCTGGATTGGTTGCGGGGAGATAGAGTTGAGTATCGCCGTTTTGCTGCTGTCTTGATTTTGAAAGAAATGGCAGAAAACGCTTCAACAGTGTTCAATGTTCATGTGCCTGAATTTGTTGATGCTATTTGGGTTGCTTTGAGGGATCCAGTGTTGCCTATTCGAGAGCGAGCTGTGGAAGCTCTACGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGCTGGCGTGTGCAATGGTATTATCGTATGTTTGAGGCTACCCAAGATGGGTTGGGTAGAAATGCTTCCATTCACAGCATACATGGTTCTTTGCTTGCAGTTGGAGAACTTTTAAGGAATACAGGTGAATTTATGATGTCTAGGTACAGGGAAGTTGCCGAAATTGTCCTTAGATATCTTGAGCACCGGGACAGGCTGGTTCGCCTGAGCATAACTTCGCTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTCACAAACTACTTAACGATATGTATGAATCATATTCTTGCAGTTTTGCGAATACCGGCAGAACGCTCCAGTGGTTTTATTGCCCTTGGGGAGATGGCTGGTGCTTTGGACGGGGAACTTGTCCATTATTTGCCGACAATAACGTCTCATTTACGTGATGCGATTGCTCCACGTAGAGGTAGACCCTCTCTTGAGGCTTTGGTTTGTGTTGGAAACATAGCAAAAGCAATGGGGCCGGCTATGGAACCTCATGTTCTTGGTCTTTTGGATGTTATGTTCTCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCACTTGAGCAAATAACTACCAGTATTACATCTTTGCTTCCTACCATCCAAGATCGGTTACTTGACTGCATTTCGGCGGTTCTTTCAAAATCCTACCAGTCTCAGGGAAGGTCGGCTGTTGGCATGGGTAGAGGAAATTTAATGAATGTCCCTCAGCCAGCGTCAGACCTCAGTGGTTCAGCCCTTGTGCAGCTTGCTTTGCAGACTTTAGCTCGTTTTAATTTCAAGAATCAGTTGTTGTATATTTTGGATGATGATGATGGAGCCATCCGGAAAGATGCTGCCCTATGTTGTTGCAGATTAGTTGCAAATTCCTTTTCTGGTATGCAATATGCATCTGGTAGGGCAAATCGGGGAAAGCGACGACGTCTTATTGAGGAGATTGTGGAAAAGCTTCTCAGTGAAGCTGTTGCAGATGCTGATGTTATTGTTCGTCATTCAATATTTTCCTCCCTTCATGGGAATAGAGGTTTTGATGATTTTTTGGCGCAGGCTGATAGTCTGAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGAGGTTCGAGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAACCCTGCTTATGTTCTTCCAGCACTTCGTCGGCATCTTATACAGTTGTTGACTTACCTGGGGCAGAGTGCAGATACCAAATGCCGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGAAACTGTGAACGACTAATACTTCCATACATTGCTCCGATACACAAGGCACTTGTTGCAAGACTTAATGATGGCACTGGTGTGAACGCAAATAATGGCATTATTAGTGGAGTTCTTGTAACTGTTGGGGATCTGGCTAGAGTGGGTGGTTTTGCAATGCGAAAATACATTCCTGAGCTTATGCCTTTAATTGTTGATGCTTTATTGGATGGAGCAGCCGTCACCAAACGGGAAGTGGCTGTCGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCATACAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTACTGAATGGTGAATTAGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTCGGAATTATGGGCGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCCTGCCTGGGTCACATGGAGATGTTACCCGTAGTGCGAGTGAATCTGGTCAACACTTTCAGTCTGTGGATGAATTGCCTATGGACCTTTGGCCATCATTTGCAACCTCAGAAGACTATTATTCTACGGTTGCAATCAACTCGCTCATGCGTATACTTAGGGACCCTTCACTTGCTACTTACCACCTAAAGGTGGTCGGATCTCTTATGTTCATATTCAAGTCAATGGGTCTTGGTTGTGTTCCATACTTACCAAAGGTTTTACCTGATCTCTTACATATAGTCCGGACATGTGATGATGCTTTAAAGGATTTTATAACATGGAAGCTTGGCACACTAGTGTCTATTGTGCGTCAGCATATTCGGAAATATCTGCATGAGTTGCTCGTTCTAATTTCAGAACTATGGTCAACCTTCAGTTTTCCTGCTGCTGGCCGTCCTCAACTTGGTTATCCAGTTCTGCATCTGGTGGAGCAGCTTTGCTTGGCTCTCAATGATGAATTTCGAACATACCTTCCTGATATTCTTCCATGTTGTATTCAAGTTCTAAGAACATTGGATGAACATATGCATCTTCTTCTTCCTGCACTCATTCGCTTGTTTAAAGTTGATGCTTCAGTGGACATAAGACGTGCTGCTATTAAAACTTTGACGAAACTTATTCCTCGTGTGCAGGTTGCTGGTCACATATCTTCTCTCGTGCATCACTTAAAGCTAGTGTTAGATGGCAAGAACGATGAGCTCCGGAAGGATGCTGTTGATGCCCTTTGTTGTCTAGCTCATGCTCTTGGGGAGGACTTCACAATTTTTATCCCATCAATACACAAACTTCTGCTGAAACATCGTTTAAGGCATAAGGAATTTGAGGAAATTGAAGGGCGGTTGAAGCGACGTGAACCTCTAATTTTGGGGAGTACAACTTTTCAAAGATTAAGCAGACGGCTTCCAGTAGAGGTTATTACTGACCGTTGGAATGATTTAGAGATTGACCTTTATGATAATGGGTCAGATATGCAGAAACAACTTCGAGGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACATTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCCCTACGAACCTGTGCAAGGCTTGCACAGTTGCAGCCATTTGTTGGGCGGGAGCTATTTGCAGCTGGTTTTGTCAGCTGTTGGGCACAGCTGAACGAGACTAGTCAGAAACAGTTAGTTCGAAGTTTGGAGATGGCATTTTCATCCCCAAATATACCTCCTGAAATTTTGGCAACACTTCTTAATTTGGCAGAGTTCATGGAACATGATGAGAAGCCTCTTCCTATAGACATTCGCCTGCTTGGTGCTCTTGCAGAAAAGTGTCGTGCATTTGCAAAGGCATTGCATTACAAAGAAATGGAATTTGAAGGAGCTCGCTCCAAGAAGATGGATGCCAACCCTGTTGCTGTAGTTGAAGCACTTATACATATAAACAACCAGTTACACCAGCATGAGGCTGCAGTTGGAATATTGACCTTTGCCCAACAAAATATGGATGTCCAACTCAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGACGATGCTCTCAAGGCCTACACAGCAAAAGCCTCTCAAGCGTCGAGTCCACATCTTGTTTTGGATGCCACTTTAGGTCGGATGCGATGCCTTGCTGCTTTGGCACAATGGGAGGAGCTTAACAACCTGTGCAAGGAATATTGGACCCCAGCTGAACCGGCTGCTCGATTGGAAATGGCACCAATGGCTGCTAATGCTGCTTGGAACATGGGGGAGTGGGATCAAATGGCAGAATATGTGTCGCGGTTAGATGATGGTGATGAAACCAAACTTAGGGGCTTGGGGAACACTGCTGCTAGTGGTGATGGAAGCAGTAATGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGAGGAAAGTATGATGAAGCACGCGAGTATGTTGAAAGAGCTAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATAGCAATATGGTCCGTGTTCAGCAGCTGTCAGAACTTGAAGAGGTCATTGATTATTGCACACTTCCTCTGGGAAATCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGTACTTTTAGCAGTTAGAGCACTTGTTCTACCTCCGACAGAAGATGTTGATACTTGGCTTAAGTTTGCTTCCCTTTGTCGGAAAAGTGGGCGAATTAGTCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTATGATCCTGAAGATGTTACTCATGAAAGCCTGCGGTACGATGGGCATCCACAAGTGATGCTTGCATATTTGAAGTACCAATGGTCGCTTGGAGAGGATGTTAAGCGCAAGGAAGCATTTGCAAGGCTACAGAATTTGGCAATAGAGCTTTCAAGTCCACCTAGTATTCAACCAGTTACACCGACTGGCTTGATGAGCTCTAGTAGTCCAAGTGTTCCACTTATCGCCCGTGTGTATCTCAAATTGGGAGTCTGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCTCAATGCCTTCCTCACTGCAACTCAATGTGCCAATAAATGGGCTAAGGCATGGCACAAGTGGGCACTGTTTAATACAGCAGTGATGTCACTTTATACTGTAAGAGGTTATGCAAGTGTCGCGTCACAGTTTGTTGTTGCAGCGGTCACTGGATATTTTCACTCAATAGCATGTTCAGCGAATACCAGAGGTGTTGATGATAGTTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTTAACCATGGAGCTACTGCAGAAGTGCAAATGGCACTACAGAAAGGGTTTGCACATGTTAATATTAACACATGGCTGGTGGTTTTGCCTCAAATAATCGCCAGGATACATTCTAATAACTATGCTGTGAGGGAACTGATACAGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGGCTCTTATGTACCCTCTTCTTGTAGCATGTAAATCAATAAGTAATTTACGTAGAGCTGCAGCTCATGAAGTGGTTGACAAAGTTCGACAGCATAGTGGTGTGCTTGTGGATCAGGCACAACTTGTGTCAAAGGAACTAATCCGGGTCGCAATACTTTGGCATGAAATGTGGCATGAGGCTCTGGAAGAAGCTAGTCGCCTGTATTTTGGCGAACATAACATTGAAGGAATGCTAAAAGTATTGGAGCCACTGCATGAAATACTTGAGGAAGGAGCTAAAAAGAACAAAACCACCATAAAAGAGAGAGCATTCATTGAGGCATATCGTCATGAATTGCTGGAGGCCTACGACTGTTGCATGAAATATAAGAGAACAGGGAAAGATGCTGAGCTTACTCAGGCTTGGGATCTTTATTATCATGTATTTAGACGAATAGATAAGCAGCTGCAGAGTCTTACTACACTGGACTTAGAGTCCGTCTCACCAGAGTTGTTGGAATGTCGTAATTTGGAGCTTGCTGTTCCTGGCACATATCGTGCTGAATCTCCAGTTGTAACAATTGCATCATTTGCGCGCCAACTTGTTGTCATAACATCCAAACAAAGACCAAGGAAATTGACGATTCATGGGAATGATGGTGAGGATTATGCCTTCTTGCTGAAGGGACATGAGGATTTGCGCCAAGATGAACGCGTCATGCAGCTTTTTGGTTTGGTGAATACGCTACTGGAGAATTCAAGAAAAACTGAGGAAAAGGATCTCTCCATTGAACGATATTCTGTCATTCCATTGTCTCCAAACAGTGGACTAATTGGTTGGGTTCCTAATTGTGACACCCTTCACTATCTCATCAGGGAGTACAGAGATGCCAGAAAGATCACTCTAAATCAAGAACACAAGTATATGCTAAGTTTTGCTCCGGATTATGATCATTTGCCACTCATAGCTAAAGTGGAAGTATTTGAGTATGCCCTGCAAAATACAGAGGGAAATGACCTTGCAAGGGTTCTTTGGCTGAAAAGTCGCACTTCTGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTAGCAGTCATGAGCATGGTTGGGTACCTTCTTGGCTTAGGTGATCGCCACCCAAGCAATCTTATGCTGAACCGCTATAGTGGGAAGATCTTGCACATTGATTTTGGGGACTGCTTTGAGGCCTCTATGAACCGGGAAAAGTTTCCTGAGAAGGTCCCCTTTCGGTTGACTAGAATGCTTGTGAAAGCAATGGAAGTTAGTGGTATAGAGGGCAACTTCCGTTCAACTTGTCAAAATGTGATGCAAGTTCTACGAACACATAAAGATAGCGTTATGGCAATGATGGAGGCCTTTGTCCACGATCCTCTCATCAATTGGCGTCTCTTCAACTTCAATGAAGTTCCTCAAATGTCAATGCTTGGAAATAATAATGTTACTCCTGTTGTGGATGCTGAAGAACCTGCTCAAAATAAAGAGCTTCCCCACCCTCAACGAGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTCAATCAACTGGGTGATGCTAATGAGGTCTTGAATGAGCGTGCTGTGGTTGTCATGGCTCGGATGAGTAATAAACTTACTGGACGTGACTTTTCTACCGGCTCCACTGTAGCTTCCAGTTCTATTCATGCAATTGACCACAGCACGCTGATTTCTGGAGAGAGTCGAGAAGTTGATCATGGTTTATCTGTTAAGCTGCAAGTTCAAAAGTTGATTATCCAAGCATCATCTCACGAAAATTTATGCCAAAATTATGTCGGGTGGTGTCCGTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

2438

Amino Acids

273.81

Weight (kDa)

6.74

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 749 - 917 1.7e-52 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1404 - 1771 8.7e-97 FAT domain
HEAT_ATR PF23593 1786 - 1865 1.8e-10 Serine/threonine-protein kinase ATR-like, HEAT repeats
FRB_dom PF08771 1878 - 1980 1.2e-37 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 2049 - 2297 1.1e-81 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2407 - 2437 2.4e-13 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2714
AasI GACNNNNNNGTC 1 cut(s) 1090
AatII GACGTC 1 cut(s) 1627
Acc36I ACCTGC 3 cut(s) 3061, 4636, 6979
Acc65I GGTACC 1 cut(s) 6594
AccB1I GGYRCC 2 cut(s) 4210, 6594
AccB7I CCANNNNNTGG 3 cut(s) 3596, 5121, 6302
AccBSI CCGCTC 1 cut(s) 4609
AccI GTMKAC 3 cut(s) 1129, 2321, 2551
AccII CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
AccIII TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
AcoI YGGCCR 2 cut(s) 2489, 2830
AcuI CTGAAG 8 cut(s) 2797, 2997, 3554, 4590, 4809, 6179, 6984, 7013
AcyI GRCGYC 2 cut(s) 1624, 6889
AflII CTTAAG 1 cut(s) 4706
AflIII ACRYGT 3 cut(s) 2687, 5374, 6190
AhdI GACNNNNNGTC 1 cut(s) 5971
AhlI ACTAGT 2 cut(s) 2734, 3653
AjiI CACGTC 4 cut(s) 2330, 3029, 3290, 7121
AjnI CCWGG 5 cut(s) 1902, 2404, 5050, 5420, 6022
AleI CACNNNNGTG 2 cut(s) 2045, 2735
AloI GAACNNNNNNTCC 4 cut(s) 1208, 1240, 2896, 2928
Alw21I GWGCWC 9 cut(s) 268, 876, 2237, 3141, 3802, 3868, 4669, 4979, 7012
Alw26I GTCTC 8 cut(s) 680, 1098, 1362, 3505, 3644, 5983, 6297, 6896
AlwNI CAGNNNCTG 3 cut(s) 30, 1676, 4510
Ama87I CYCGRG 1 cut(s) 10
Aor13HI TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
ApaI GGGCCC 1 cut(s) 4585
ArsI GACNNNNNNTTYG 2 cut(s) 5013, 5045
Asp700I GAANNNNTTC 4 cut(s) 754, 1650, 6711, 7026
Asp718I GGTACC 1 cut(s) 6594
AspLEI GCGC 9 cut(s) 76, 1747, 1766, 2363, 3480, 4887, 6073, 6075, 6180
AsuC2I CCSGG 5 cut(s) 39, 878, 1931, 5642, 6704
AsuII TTCGAA 4 cut(s) 326, 2902, 3673, 4381
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 6 cut(s) 50, 2482, 2560, 4176, 4495, 6724
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 3 cut(s) 3640, 4585, 5149
BalI TGGCCA 1 cut(s) 2491
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 2 cut(s) 4210, 6594
BanII GRGCYC 6 cut(s) 268, 3141, 3868, 4585, 4979, 5044
BarI GAAGNNNNNNTAC 4 cut(s) 739, 771, 3900, 3932
BauI CACGAG 3 cut(s) 3063, 3095, 7011
BbsI GAAGAC 2 cut(s) 2517, 5297
Bbv12I GWGCWC 9 cut(s) 268, 876, 2237, 3141, 3802, 3868, 4669, 4979, 7012
BbvCI CCTCAGC 2 cut(s) 173, 1410
BceAI ACGGC 4 cut(s) 492, 2168, 2817, 3353
BcgI CGANNNNNNTGC 2 cut(s) 2520, 2554
BciT130I CCWGG 5 cut(s) 1904, 2406, 5052, 5422, 6024
BciVI GTATCC 2 cut(s) 4605, 5418
BclI TGATCA 2 cut(s) 6427, 7210
BcnI CCSGG 5 cut(s) 39, 878, 1931, 5642, 6704
BcoDI GTCTC 8 cut(s) 680, 1098, 1362, 3505, 3644, 5983, 6297, 6896
BcuI ACTAGT 2 cut(s) 2734, 3653
BfoI RGCGCY 1 cut(s) 2364
BfrI CTTAAG 1 cut(s) 4706
BfuAI ACCTGC 3 cut(s) 3061, 4636, 6979
BfuI GTATCC 2 cut(s) 4605, 5418
BglII AGATCT 2 cut(s) 4754, 6657
BlpI GCTNAGC 1 cut(s) 5886
BmcAI AGTACT 1 cut(s) 2337
Bme18I GGWCC 6 cut(s) 50, 2482, 2560, 4176, 4495, 6724
BmeRI GACNNNNNGTC 1 cut(s) 5971
BmeT110I CYCGRG 1 cut(s) 10
BmgBI CACGTC 4 cut(s) 2330, 3029, 3290, 7121
BmrI ACTGGG 5 cut(s) 48, 311, 1961, 2247, 7055
BmuI ACTGGG 5 cut(s) 48, 311, 1961, 2247, 7055
BpiI GAAGAC 2 cut(s) 2517, 5297
BplI GAGNNNNNCTC 4 cut(s) 299, 331, 5963, 5995
BpmI CTGGAG 7 cut(s) 254, 1000, 5858, 6030, 6248, 6563, 7212
Bpu10I CCTNAGC 6 cut(s) 173, 896, 1410, 2139, 5127, 6607
Bpu1102I GCTNAGC 1 cut(s) 5886
Bpu14I TTCGAA 4 cut(s) 326, 2902, 3673, 4381
BpuEI CTTGAG 6 cut(s) 890, 1160, 1283, 4028, 4053, 5783
BpuMI CCSGG 5 cut(s) 39, 878, 1931, 5642, 6704
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 4 cut(s) 656, 1103, 1121, 5549
BsaBI GATNNNNATC 2 cut(s) 4824, 6426
BsaHI GRCGYC 2 cut(s) 1624, 6889
BsaWI WCCGGW 5 cut(s) 373, 1527, 2682, 3141, 6418
BsaXI ACNNNNNCTCC 8 cut(s) 480, 510, 2067, 2097, 2169, 2199, 4863, 4893
Bse118I RCCGGY 4 cut(s) 1003, 1183, 4189, 7133
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 8 cut(s) 438, 710, 1182, 1995, 2127, 3833, 6765, 6855
Bse8I GATNNNNATC 2 cut(s) 4824, 6426
BseAI TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
BseBI CCWGG 5 cut(s) 1904, 2406, 5052, 5422, 6024
BseCI ATCGAT 1 cut(s) 943
BseJI GATNNNNATC 2 cut(s) 4824, 6426
BseMI GCAATG 8 cut(s) 438, 710, 1182, 1995, 2127, 3833, 6765, 6855
BsePI GCGCGC 1 cut(s) 6071
BseRI GAGGAG 3 cut(s) 1649, 4160, 6562
BseSI GKGCMC 3 cut(s) 3640, 4585, 5149
BseYI CCCAGC 3 cut(s) 1826, 3461, 4180
BsgI GTGCAG 5 cut(s) 85, 1466, 1935, 2967, 3089
Bsh1236I CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
BshNI GGYRCC 2 cut(s) 4210, 6594
BshVI ATCGAT 1 cut(s) 943
BsiHKAI GWGCWC 9 cut(s) 268, 876, 2237, 3141, 3802, 3868, 4669, 4979, 7012
BsiHKCI CYCGRG 1 cut(s) 10
BslFI GGGAC 8 cut(s) 4, 893, 1391, 2573, 4046, 6177, 6692, 6710
BsmAI GTCTC 8 cut(s) 680, 1098, 1362, 3505, 3644, 5983, 6297, 6896
BsmBI CGTCTC 3 cut(s) 1098, 5983, 6896
BsmFI GGGAC 8 cut(s) 4, 893, 1391, 2573, 4046, 6177, 6692, 6710
BsmI GAATGC 3 cut(s) 5733, 5807, 6750
BsoBI CYCGRG 1 cut(s) 10
Bsp119I TTCGAA 4 cut(s) 326, 2902, 3673, 4381
Bsp120I GGGCCC 1 cut(s) 4581
Bsp13I TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
Bsp1407I TGTACA 1 cut(s) 2227
Bsp1720I GCTNAGC 1 cut(s) 5886
Bsp19I CCATGG 1 cut(s) 5326
BspDI ATCGAT 1 cut(s) 943
BspEI TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
BspFNI CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
BspHI TCATGA 4 cut(s) 4801, 5563, 5827, 6579
BspMAI CTGCAG 5 cut(s) 442, 3955, 5341, 5560, 5949
BspMI ACCTGC 3 cut(s) 3061, 4636, 6979
BspT104I TTCGAA 4 cut(s) 326, 2902, 3673, 4381
BspT107I GGYRCC 2 cut(s) 4210, 6594
BspTI CTTAAG 1 cut(s) 4706
BsrBI CCGCTC 1 cut(s) 4609
BsrDI GCAATG 8 cut(s) 438, 710, 1182, 1995, 2127, 3833, 6765, 6855
BsrFI RCCGGY 4 cut(s) 1003, 1183, 4189, 7133
BsrGI TGTACA 1 cut(s) 2227
BssAI RCCGGY 4 cut(s) 1003, 1183, 4189, 7133
BssHII GCGCGC 1 cut(s) 6071
BssNAI GTATAC 1 cut(s) 2552
BssNI GRCGYC 2 cut(s) 1624, 6889
BssSI CACGAG 3 cut(s) 3063, 3095, 7011
BssT1I CCWWGG 6 cut(s) 117, 318, 664, 1033, 5326, 6107
Bst1107I GTATAC 1 cut(s) 2552
Bst2BI CACGAG 3 cut(s) 3063, 3095, 7011
Bst2UI CCWGG 5 cut(s) 1904, 2406, 5052, 5422, 6024
Bst6I CTCTTC 5 cut(s) 3777, 4378, 4518, 5520, 6899
BstACI GRCGYC 2 cut(s) 1624, 6889
BstAFI CTTAAG 1 cut(s) 4706
BstAPI GCANNNNNTGC 7 cut(s) 437, 1772, 2021, 2401, 2779, 3575, 6068
BstAUI TGTACA 1 cut(s) 2227
BstBAI YACGTR 4 cut(s) 656, 1103, 1121, 5549
BstBI TTCGAA 4 cut(s) 326, 2902, 3673, 4381
BstDSI CCRYGG 2 cut(s) 106, 5326
BstENI CCTNNNNNAGG 3 cut(s) 3568, 4163, 7002
BstFNI CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
BstH2I RGCGCY 1 cut(s) 2364
BstHHI GCGC 9 cut(s) 76, 1747, 1766, 2363, 3480, 4887, 6073, 6075, 6180
BstMAI GTCTC 8 cut(s) 680, 1098, 1362, 3505, 3644, 5983, 6297, 6896
BstNI CCWGG 5 cut(s) 1904, 2406, 5052, 5422, 6024
BstNSI RCATGY 4 cut(s) 2691, 5256, 5378, 5529
BstSLI GKGCMC 3 cut(s) 3640, 4585, 5149
BstSNI TACGTA 1 cut(s) 5549
BstUI CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
BstV2I GAAGAC 2 cut(s) 2517, 5297
BstX2I RGATCY 8 cut(s) 613, 2095, 2368, 2597, 4754, 5905, 6256, 6657
BstXI CCANNNNNNTGG 1 cut(s) 3880
BstYI RGATCY 8 cut(s) 613, 2095, 2368, 2597, 4754, 5905, 6256, 6657
BstZ17I GTATAC 1 cut(s) 2552
Bsu15I ATCGAT 1 cut(s) 943
Bsu36I CCTNAGG 1 cut(s) 23
BsuI GTATCC 2 cut(s) 4605, 5418
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 2 cut(s) 106, 5326
BtgZI GCGATG 2 cut(s) 4042, 4132
BtrI CACGTC 4 cut(s) 2330, 3029, 3290, 7121
BtsI GCAGTG 6 cut(s) 247, 3456, 4320, 5097, 5169, 5747
BveI ACCTGC 3 cut(s) 3061, 4636, 6979
CaiI CAGNNNCTG 3 cut(s) 30, 1676, 4510
CciI TCATGA 4 cut(s) 4801, 5563, 5827, 6579
CfoI GCGC 9 cut(s) 76, 1747, 1766, 2363, 3480, 4887, 6073, 6075, 6180
Cfr10I RCCGGY 4 cut(s) 1003, 1183, 4189, 7133
ClaI ATCGAT 1 cut(s) 943
CseI GACGC 7 cut(s) 80, 1407, 2738, 4062, 5193, 6181, 6878
CspCI CAANNNNNGTGG 2 cut(s) 1803, 1838
DraI TTTAAA 3 cut(s) 1782, 2703, 3001
DrdI GACNNNNNNGTC 1 cut(s) 1090
DriI GACNNNNNGTC 1 cut(s) 5971
DseDI GACNNNNNNGTC 1 cut(s) 1090
EaeI YGGCCR 2 cut(s) 2489, 2830
Eam1104I CTCTTC 5 cut(s) 3777, 4378, 4518, 5520, 6899
Eam1105I GACNNNNNGTC 1 cut(s) 5971
EarI CTCTTC 5 cut(s) 3777, 4378, 4518, 5520, 6899
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 4 cut(s) 266, 3139, 3866, 4977
Eco105I TACGTA 1 cut(s) 5549
Eco130I CCWWGG 6 cut(s) 117, 318, 664, 1033, 5326, 6107
Eco147I AGGCCT 4 cut(s) 4049, 5843, 6692, 6860
Eco24I GRGCYC 6 cut(s) 268, 3141, 3868, 4585, 4979, 5044
Eco32I GATATC 1 cut(s) 866
Eco47I GGWCC 6 cut(s) 50, 2482, 2560, 4176, 4495, 6724
Eco53kI GAGCTC 4 cut(s) 266, 3139, 3866, 4977
Eco57I CTGAAG 8 cut(s) 2797, 2997, 3554, 4590, 4809, 6179, 6984, 7013
Eco81I CCTNAGG 1 cut(s) 23
Eco88I CYCGRG 1 cut(s) 10
EcoICRI GAGCTC 4 cut(s) 266, 3139, 3866, 4977
EcoNI CCTNNNNNAGG 3 cut(s) 3568, 4163, 7002
EcoO109I RGGNCCY 3 cut(s) 2560, 4582, 6724
EcoRI GAATTC 2 cut(s) 310, 6232
EcoRII CCWGG 5 cut(s) 1902, 2404, 5050, 5420, 6022
EcoRV GATATC 1 cut(s) 866
EcoT14I CCWWGG 6 cut(s) 117, 318, 664, 1033, 5326, 6107
EcoT22I ATGCAT 2 cut(s) 1594, 2970
EcoT38I GRGCYC 6 cut(s) 268, 3141, 3868, 4585, 4979, 5044
ErhI CCWWGG 6 cut(s) 117, 318, 664, 1033, 5326, 6107
Esp3I CGTCTC 3 cut(s) 1098, 5983, 6896
FalI AAGNNNNNCTT 6 cut(s) 4824, 4856, 6511, 6543, 6713, 6745
FaqI GGGAC 8 cut(s) 4, 893, 1391, 2573, 4046, 6177, 6692, 6710
FauI CCCGC 2 cut(s) 477, 3594
FauNDI CATATG 1 cut(s) 2964
FbaI TGATCA 2 cut(s) 6427, 7210
FblI GTMKAC 3 cut(s) 1129, 2321, 2551
FriOI GRGCYC 6 cut(s) 268, 3141, 3868, 4585, 4979, 5044
GlaI GCGC 9 cut(s) 75, 1746, 1765, 2362, 3479, 4886, 6072, 6074, 6179
GsaI CCCAGC 3 cut(s) 1830, 3465, 4184
GsuI CTGGAG 7 cut(s) 254, 1000, 5858, 6030, 6248, 6563, 7212
HaeII RGCGCY 1 cut(s) 2364
HgaI GACGC 7 cut(s) 80, 1407, 2738, 4062, 5193, 6181, 6878
HhaI GCGC 9 cut(s) 76, 1747, 1766, 2363, 3480, 4887, 6073, 6075, 6180
Hin1I GRCGYC 2 cut(s) 1624, 6889
Hin6I GCGC 9 cut(s) 74, 1745, 1764, 2361, 3478, 4885, 6071, 6073, 6178
HinP1I GCGC 9 cut(s) 74, 1745, 1764, 2361, 3478, 4885, 6071, 6073, 6178
HincII GTYRAC 5 cut(s) 1896, 2450, 2808, 5575, 6738
HindII GTYRAC 5 cut(s) 1896, 2450, 2808, 5575, 6738
HindIII AAGCTT 2 cut(s) 1649, 2723
Hpy99I CGWCG 6 cut(s) 1623, 1626, 1878, 3291, 4078, 4581
Hsp92I GRCGYC 2 cut(s) 1624, 6889
HspAI GCGC 9 cut(s) 74, 1745, 1764, 2361, 3478, 4885, 6071, 6073, 6178
KflI GGGWCCC 1 cut(s) 2560
Kpn2I TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
KpnI GGTACC 1 cut(s) 6598
KroI GCCGGC 1 cut(s) 1183
KroNI GCCGGC 1 cut(s) 1185
Ksp22I TGATCA 2 cut(s) 6427, 7210
MbiI CCGCTC 1 cut(s) 4609
MfeI CAATTG 3 cut(s) 6057, 6883, 7167
MflI RGATCY 8 cut(s) 613, 2095, 2368, 2597, 4754, 5905, 6256, 6657
MlsI TGGCCA 1 cut(s) 2491
MluI ACGCGT 1 cut(s) 6190
MluNI TGGCCA 1 cut(s) 2491
MlyI GAGTC 6 cut(s) 4013, 4087, 5037, 5959, 5981, 7207
Mox20I TGGCCA 1 cut(s) 2491
Mph1103I ATGCAT 2 cut(s) 1594, 2970
MroI TCCGGA 5 cut(s) 373, 1527, 2682, 3141, 6418
MroNI GCCGGC 1 cut(s) 1183
MroXI GAANNNNTTC 4 cut(s) 754, 1650, 6711, 7026
MscI TGGCCA 1 cut(s) 2491
MslI CAYNNNNRTG 8 cut(s) 1101, 2045, 2418, 2735, 3942, 5595, 6129, 6584
Msp20I TGGCCA 1 cut(s) 2491
MspA1I CMGCKG 8 cut(s) 177, 3617, 3630, 3643, 4184, 4510, 5225, 5944
MspCI CTTAAG 1 cut(s) 4706
MunI CAATTG 3 cut(s) 6057, 6883, 7167
Mva1269I GAATGC 3 cut(s) 5733, 5807, 6750
MvaI CCWGG 5 cut(s) 1904, 2406, 5052, 5422, 6024
MvnI CGCG 7 cut(s) 74, 193, 4276, 4407, 5204, 6073, 6192
NaeI GCCGGC 1 cut(s) 1185
NciI CCSGG 5 cut(s) 39, 878, 1931, 5642, 6704
NcoI CCATGG 1 cut(s) 5326
NdeI CATATG 1 cut(s) 2964
NgoMIV GCCGGC 1 cut(s) 1183
NsiI ATGCAT 2 cut(s) 1594, 2970
NspI RCATGY 4 cut(s) 2691, 5256, 5378, 5529
NspV TTCGAA 4 cut(s) 326, 2902, 3673, 4381
OliI CACNNNNGTG 2 cut(s) 2045, 2735
PagI TCATGA 4 cut(s) 4801, 5563, 5827, 6579
PauI GCGCGC 1 cut(s) 6071
PceI AGGCCT 4 cut(s) 4049, 5843, 6692, 6860
PciI ACATGT 2 cut(s) 2687, 5374
PcsI WCGNNNNNNNCGW 2 cut(s) 1615, 7009
PctI GAATGC 3 cut(s) 5733, 5807, 6750
PdiI GCCGGC 1 cut(s) 1185
PdmI GAANNNNTTC 4 cut(s) 754, 1650, 6711, 7026
PfeI GAWTC 7 cut(s) 976, 1486, 2441, 3539, 5066, 6041, 6121
PflFI GACNNNGTC 2 cut(s) 1623, 5487
PflMI CCANNNNNTGG 3 cut(s) 3596, 5121, 6302
PfoI TCCNGGA 1 cut(s) 5050
PleI GAGTC 6 cut(s) 4012, 4086, 5036, 5958, 5980, 7206
PpsI GAGTC 6 cut(s) 4012, 4086, 5036, 5958, 5980, 7206
Ppu21I YACGTR 4 cut(s) 656, 1103, 1121, 5549
PpuMI RGGWCCY 2 cut(s) 2560, 6724
PscI ACATGT 2 cut(s) 2687, 5374
PsiI TTATAA 1 cut(s) 2714
Psp124BI GAGCTC 4 cut(s) 268, 3141, 3868, 4979
Psp5II RGGWCCY 2 cut(s) 2560, 6724
Psp6I CCWGG 5 cut(s) 1902, 2404, 5050, 5420, 6022
PspFI CCCAGC 3 cut(s) 1826, 3461, 4180
PspGI CCWGG 5 cut(s) 1902, 2404, 5050, 5420, 6022
PspOMI GGGCCC 1 cut(s) 4581
PspPPI RGGWCCY 2 cut(s) 2560, 6724
PsrI GAACNNNNNNTAC 4 cut(s) 1968, 2000, 2328, 2360
PstI CTGCAG 5 cut(s) 442, 3955, 5341, 5560, 5949
PstNI CAGNNNCTG 3 cut(s) 30, 1676, 4510
PsuI RGATCY 8 cut(s) 613, 2095, 2368, 2597, 4754, 5905, 6256, 6657
PsyI GACNNNGTC 2 cut(s) 1623, 5487
PteI GCGCGC 1 cut(s) 6071
PvuII CAGCTG 6 cut(s) 3617, 3630, 3643, 4184, 4510, 5944
RseI CAYNNNNRTG 8 cut(s) 1101, 2045, 2418, 2735, 3942, 5595, 6129, 6584
SacI GAGCTC 4 cut(s) 268, 3141, 3868, 4979
ScaI AGTACT 1 cut(s) 2337
SchI GAGTC 6 cut(s) 4013, 4087, 5037, 5959, 5981, 7207
SfuI TTCGAA 4 cut(s) 326, 2902, 3673, 4381
SinI GGWCC 6 cut(s) 50, 2482, 2560, 4176, 4495, 6724
SmiMI CAYNNNNRTG 8 cut(s) 1101, 2045, 2418, 2735, 3942, 5595, 6129, 6584
SmlI CTYRAG 7 cut(s) 869, 1139, 1262, 4043, 4068, 4706, 5762
SmoI CTYRAG 7 cut(s) 869, 1139, 1262, 4043, 4068, 4706, 5762
SnaBI TACGTA 1 cut(s) 5549
SpeI ACTAGT 2 cut(s) 2734, 3653
SseBI AGGCCT 4 cut(s) 4049, 5843, 6692, 6860
SspI AATATT 4 cut(s) 1701, 3963, 4172, 5383
SstI GAGCTC 4 cut(s) 268, 3141, 3868, 4979
StuI AGGCCT 4 cut(s) 4049, 5843, 6692, 6860
StyI CCWWGG 6 cut(s) 117, 318, 664, 1033, 5326, 6107
TatI WGTACW 4 cut(s) 2227, 2335, 3311, 6357
TauI GCSGC 2 cut(s) 8, 36
TfiI GAWTC 7 cut(s) 976, 1486, 2441, 3539, 5066, 6041, 6121
TspGWI ACGGA 5 cut(s) 361, 4487, 5965, 6779, 7293
Tth111I GACNNNGTC 2 cut(s) 1623, 5487
Van91I CCANNNNNTGG 3 cut(s) 3596, 5121, 6302
Vha464I CTTAAG 1 cut(s) 4706
VpaK11BI GGWCC 6 cut(s) 50, 2482, 2560, 4176, 4495, 6724
XagI CCTNNNNNAGG 3 cut(s) 3568, 4163, 7002
XbaI TCTAGA 1 cut(s) 2365
XceI RCATGY 4 cut(s) 2691, 5256, 5378, 5529
XcmI CCANNNNNNNNNTGG 2 cut(s) 2197, 4865
XmiI GTMKAC 3 cut(s) 1129, 2321, 2551
XmnI GAANNNNTTC 4 cut(s) 754, 1650, 6711, 7026
ZraI GACGTC 1 cut(s) 1625
ZrmI AGTACT 1 cut(s) 2337
Zsp2I ATGCAT 2 cut(s) 1594, 2970
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.