Rroxscaffold_179G00449330

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000179
OG0005708
Physical Location & Seq
Reverse (-)
28452 .. 49791
21340 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_179G00449330.1

Sequence Viewer

Length: 5892 bp
ATGGTTCAGGGTCACGACCTTCTTGAATTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGACGGAGCTATCCGAAAAGATGCTGCCCTATGTTTTAACAGGAAAATGGCCTCTGTGCAGATTGTTGAAAAGCTTCTCATTGAGGCTGTTGCAGATGCTGATGTGATTGTTCGCCATTCAATATTTTCTTCTCTACATGGGAATAGAGGCTTTGACGATTTCCTGGCTCAGGCTGACAGTCTCAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGCGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCTTATGTTCTTCCAGCTCTTCGCCGCCATCTTATACAGTTGTTGACTTACTTGGGTCAGAGTGCAGATAGCAAATGCAGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGTAACTGTGAAAGACTAATACTTCCGTACATTGCTCCTATACACAAGGCACTTGTGGCAAGACTTGTGGATGGCACTGGTGTCGGCTCGAATAATGGCATCATTAGTGGAGTTCTTGTAACTGTTGGGGATCTTGCTAGAGTGGGTGGGTTTGCAATGAGAAAATATATACGAGAGCTTATGCCTTTGATCGTTGAAGCTTTATTGGATGGAGCGGCTGTCACAAAACGGGAAGTGGCTGTAGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCGTATAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTGCTTAACGGTGAGTTGGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTTGGAATTATGGGTGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCTTGCCTGGGTCACATGGAGATGTTCCTCGTAATGCCAGTGATTCTGGTCAACACATCCAATCTGTGGATGAATTGCCTATGGACTTGTGGCCATCATTTGCAACCTCTGAAGATTATTATTCTACGGTTGCAATCAATTCTCTCATGCGAATACTTAGGGACCCTTCACTTGGTACTTACCACCTAAAGGTTGTCGGATCACTTATGTTCATATTCAAGGTTTTGCCTGACCTTTTCAATATAGTCCGCACATGTGATGATGCTTTGAAGGATTTTATAACATGGAAGCTTGGAACTCTAGTGTCTATTGTGCGTCAGCACATACGGAAATATCTGCATGAATTGTTGGTTCTAATTTCAGAACTATGGTCAACCTTTAGTTTCCCTGCTGGTCCCCGTCCTCAACCCGGCTATCCAGTTCTTCATCTGGTGGAGCAGCTTTGTTTGTCCCTGAATGATGAGTTCAGGACGTATCTTCCTGATATTCTTCCATGCTGTATCCAAGTTTTAAGTGACGCAGAACGGTGTAATGACTACACTTACGTTCTTGACATCCTCCATACACTTGAAGTATTTAGTGATAATCATTCCAATTTCCAAAATATAGGAACATTGGATGAACATATGCATCTTCTTCTTCCCGCTCTAATCCGTTTGTTCAAAGTGGATGCTTCAGTGGATATAAGACGTGCTGCTATCAAAACTTTGACCAAACTCATCCCTCGGGTGCAGGTTACTGGTCACATATCTTCTCTTGTGCATCACTTGAAGCTAGTCTTAGATGGGAAGGATGACCTCCGGAAGGACACTGTCGATGCGCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGATTTCACCATCTTTATCCCATCCATACACAAACTTTTGTTAAAACATCGCTTACGGCACAAGGGGTTTGAGGAAATTGAAGGCCATTTGCAGCGACGTGAACCTTTGGCTCCCCAAAGATTAAGTCGACGACTTCCAGAGGTTGTTGCTGACCGTTCAACTGATTTGGAGATTGACCCTTATGATGATGTGACTGATGTGCAGAAAAAACTTCGAAGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACACTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCACTACGAACCTGTGCAAGGCTTGCACAGTCGCAGCCATTTGTTGGGCGGGAGTTGTTTGCAGCTGGATTTGTTAGCTGTTGGGCACAGCTAAACGAGACTAGTCAGAAACAGTTAGTTCGGAGTTTGGAGATGGCATTTTCATCTCCAAATATTCCTCCTGAAATTTTGGCGACACTGCTTAATCTGGCAGAGTTCATGGAACATGATGAGAAACCCCTTCCTATAGACATTCGCCTACTTGGTGCTCTTGCAGAGAAGTGTCGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCACGTTCCAAGAAGATGGATGCCAATCCTGTTGCTGTCGTTGAAGCACTTATACATATAAACAACCAGTTACAACAGCATGAGGCTGCAGTTGGAATATTGACCTATGCCCAGCAAAATTTGGATGTTCAACTGAAAGAATCATGGTATGAGAAGTTGCAGCGATGGGATGATGCTCTCAAGGCCTACACTGCAAAAGCATCTCAAGCATCGAGTCAACATCTTATTTTGGATGCTACTTTAGGTCGTATGCGATGCCTTGCTGCCTTGGCTCGATGGGAAGAGCTTAACAACCTGTTTAAGGAATACTGGACCCCAGCTGAGCCTGCTGCTCGACTGGAAATGGCCCCAATGGCTGCAAGTGCTGCTTGGAATATGGGAGAGTGGGATCAAATGGCCGAATATGTGTCTCGATTGGACGATGGTGATGAGACCAAACTTAGGGGATTGGGGAACACTGCTGCTAGTGGAGATGGAAGCAGTAGTGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGATGCAAGTATGATGAAGCACGCGAGTATGTTGAGAGAGCCAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATACCAATATGGTTCGTGTTCAGCAGCTATCAGAACTTGAGGAGGTCATCGATTATTGTACACTTCCTCTGGGGAATCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAACGTTGAGGTGTGGCAGGCACTTTTAGCAGTGAGAGCACTTGTTCTACCTCCTACTGAAGATGTTGATACTTGGCTAAAGTTTGCTACCCTCTGTCGGAAAAGTGGGCGACTTAGCCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTATGATCCCGAGACATCTCATGAAAACGTGCGATACCATGGGCCTCCACAAGTGATGCTTGCATACTTGAAATACCAATGGTCACTTGGAGAAGACGTCAAACGCAAGGAAGCGTTTGCTAGGCTGCAGAATTTGGCAATGGAGCTCTCAACTTCACCTAGCATTGAATCAGTTACGCCAATTGGCTTGATGAGCTGTAGTACTCCAAGTGTTCCACTCATTGCCCGTGTGTATCTCAAACTCGGAGAATGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCGTGTTGCCTTCCGAAATGCAACTCAATGTGCAAACAAATGGGCAAAGGCTTGGCACACATGGGCATTGTTCAATACAGCTGTTATGTCTCTTTTTACTGTCAGAGGGTATGCAAGTGCTGCTTCCCAGTTTGTTGTTGCAGCAGTCACTGGATATTTTCACTCAATCGCCTGTTCAGCAAATACTAAAGGTGTTGATGATAGCTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTAGTGCTGAAGTTCAAATGGCGTTACAAAAGGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGAGGTCTTGTTTCTGTTTTATCTGTGTGTTTATGTGAGTTTGCCAGTTAGAATTAGCATGTGCTCCAGAAAGGAGACAACAAAGCGTGTTGCTCTTATGTACCCTCTCCTAGTAGCCTGTAAATCAATAAGTAATTTACGCAAAGCTGCTGCTGAAGAAGTGGTTGACAAAGTTCGACAGCATAGTGGTTTACTTGTGGATCAGGCACAACTTGTTTCAACGGAACTAATCCGGGTCGCAATACTTTGGCATGAAATGTGGCATGAGGCTCTGGAAGAAGCTAGTCGTCTGTATTTTGGTGAACATAACATTGAGGGCATGCTAAAAGTATTGGAGCCATTACATGAAATGCTCGAGGAAGGGGCTATGAGGAATAATACCACAATAAAAGAGACAACATTCATTGAGGCCTATCGTCATGAATTGCTGGAGGCTTATGAATGTTGCATGAAATATAAGAGAACTGGAAAAGATGCCGAGCTTACTCAGGCATGGGATCTTTACTATCATGTTTTCAGACGAATAGATAAGCAGCTGCAAAGTCTCACTACATTGGACTTGGAGTCTGTCTCGCCAGAGTTGTTAGAATGTCGTGATTTGGAGCTGGCTGTTCCTGGTACATATCGAGCAGAATCTCCGGTGGTGACAATTAGATCATTTGCACGCCAGCTTGTTGTCATAACATCCAAACAGCGACCAAGGAAATTAACAATTCATGGGAGTGATGGTGAGGAATATGCCTTCTTGCTGAAGGGACATGAAGACTTGCGCCAAGATGAACGTGTCATGCAGCTTTTTGGTTTGGTGAACACGCTGCTGGAGAATTCTAGAAAAACTCAAGAGAAGGATCTCTCCATTCAACGATATTCTGTCATTCCTTTATCTCCAAACAGTGGACTGATTGGTTGGGTCCCAAATTGTGACACCCTTCACCATCTCATTAGGGAGTACAGGGATGCCAGAAAGCTGCTTTGCAAGTTTTACCCAATAAAAGCAGATGTACTAACAGGATTGGTTGGGTTGTATCTTCCTTCTCCAGAAGGAGAACCAAGTTTCTTCAGCCTTGGTTTTGAGAGTTTTGCTCCGGATTATGATCATTTGCCACTCATAGCTAAAGTGGAAGTATTTGAGTATGCCCTGCACAATACAGAGGGAAATGACCTTTCAAGGGTTCTTTGGCTGAAAAGTCGCACTTCAGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTGGCAGTCATGAGCATGGTTGGTTACCTTCTTGGTCTCGGTGATCGTCACCCAAGCAATCTTATGCTGCACCGCTATAGTGGGAAGATCTTGCACATCGACTTTGGGGACTGCTTTGAAGCTTCTATGAATCGCGAAAAGTTTCCTGAGAAGGTCCCCTTTCGATTGACTAGAATGCTAGTGAAAGCAATGGAGGTTAGTGGTATAGAGGGCAACTTCCGTTCAACTTGTGAAAATGTCATGCAAGTTCTGCGAACACATAAAGATAGTGTTATGGCCATGATGGAGGCCTTTGTTCATGATCCTCTCATCAACTGGCGTCTCTTTAACTTCAATGAAGTTCCACAAGTGGCAATGCTAGCAAATAGTCATGCTCCTCCTGTTGTGGATGCCGAAGAACCGGCTCCAGCTAGAGAGCTTCCCCAACCTCAACGTGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTTAATCAACTTGGTGATGCTAATGAGGTCTTAAATGAGCGCGCTGTGGTTGTCATGGCTCGGATGAGTAATAAGCTTACTGGGCGTGATTTTTCCACCAGCTCCGCTGTATCATCTAGCTCTATTCAACATGCAGTAGACCACAGCACGCTGATTTCTGGAGATACTCGAGAAGTTGATCATGGTTTATCTGTTAAGCTGCAAGTTCAAAAGTTGATTGGCCAAGCAACTTCTTATGAAAATTTGTGCCAGAATTATGTCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

1963

Amino Acids

221.42

Weight (kDa)

6.2

Isoelectric Point (pI)

42.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 245 - 401 1.5e-39 Serine/threonine-protein kinase mTOR domain
FAT PF02259 913 - 1279 2.6e-95 FAT domain
FRB_dom PF08771 1383 - 1485 4.2e-38 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 1553 - 1827 1.8e-79 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 1938 - 1963 9.6e-08 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1166
AatII GACGTC 1 cut(s) 3405
Acc36I ACCTGC 1 cut(s) 1609
AccB7I CCANNNNNTGG 3 cut(s) 952, 2123, 4817
AccBSI CCGCTC 3 cut(s) 668, 1532, 3136
AccI GTMKAC 3 cut(s) 809, 1864, 5763
AccII CGCG 4 cut(s) 294, 2934, 5295, 5667
AccIII TCCGGA 2 cut(s) 1686, 5008
AciI CCGC 8 cut(s) 364, 668, 1135, 1530, 2128, 3136, 5233, 5730
AclI AACGTT 1 cut(s) 3159
AclWI GGATC 9 cut(s) 591, 851, 1093, 2787, 3305, 4131, 4428, 4779, 5456
AcoI YGGCCR 4 cut(s) 977, 2787, 5436, 5845
AcsI RAATTY 8 cut(s) 26, 2244, 2381, 2509, 3436, 3603, 4747, 5866
AcyI GRCGYC 2 cut(s) 3402, 5479
AflIII ACRYGT 2 cut(s) 1139, 4705
AhdI GACNNNNNGTC 1 cut(s) 4486
AhlI ACTAGT 1 cut(s) 2180
AjiI CACGTC 3 cut(s) 818, 1577, 1835
AjnI CCWGG 6 cut(s) 228, 892, 2951, 3273, 3574, 4537
Alw21I GWGCWC 8 cut(s) 725, 853, 2329, 2395, 3196, 3453, 3989, 5602
AlwI GGATC 9 cut(s) 591, 851, 1093, 2787, 3305, 4131, 4428, 4779, 5456
AlwNI CAGNNNCTG 2 cut(s) 164, 3755
Ama87I CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
Aor13HI TCCGGA 2 cut(s) 1686, 5008
ApaI GGGCCC 1 cut(s) 3112
ApoI RAATTY 8 cut(s) 26, 2244, 2381, 2509, 3436, 3603, 4747, 5866
ArsI GACNNNNNNTTYG 2 cut(s) 1846, 1878
Asp700I GAANNNNTTC 3 cut(s) 138, 5301, 5616
AspLEI GCGC 6 cut(s) 254, 1708, 2007, 4695, 5667, 5669
AspS9I GGNCC 9 cut(s) 1048, 1280, 2703, 2737, 3108, 3109, 3347, 4834, 5314
AsuC2I CCSGG 2 cut(s) 1296, 4157
AsuII TTCGAA 2 cut(s) 1951, 2908
AsuNHI GCTAGC 1 cut(s) 5518
AvaI CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
AvaII GGWCC 5 cut(s) 1048, 1280, 2703, 4834, 5314
BaeGI GKGCMC 2 cut(s) 2167, 3112
BalI TGGCCA 3 cut(s) 979, 5438, 5847
BanII GRGCYC 3 cut(s) 3112, 3453, 3568
BarI GAAGNNNNNNTAC 2 cut(s) 2427, 2459
BauI CACGAG 1 cut(s) 5601
BbsI GAAGAC 3 cut(s) 3405, 3821, 4692
Bbv12I GWGCWC 8 cut(s) 725, 853, 2329, 2395, 3196, 3453, 3989, 5602
BceAI ACGGC 1 cut(s) 1808
BcgI CGANNNNNNTGC 2 cut(s) 517, 551
BciT130I CCWGG 6 cut(s) 230, 894, 2953, 3275, 3576, 4539
BciVI GTATCC 2 cut(s) 1397, 3132
BclI TGATCA 2 cut(s) 5017, 5803
BcnI CCSGG 2 cut(s) 1296, 4157
BcuI ACTAGT 1 cut(s) 2180
BfmI CTRYAG 6 cut(s) 693, 2304, 2478, 3431, 3502, 5236
BfuAI ACCTGC 1 cut(s) 1609
BfuI GTATCC 2 cut(s) 1397, 3132
BglI GCCNNNNNGGC 1 cut(s) 2744
BglII AGATCT 2 cut(s) 3281, 5247
BlpI GCTNAGC 1 cut(s) 2712
BmcAI AGTACT 2 cut(s) 825, 3508
Bme1390I CCNGG 8 cut(s) 230, 894, 1296, 2953, 3275, 3576, 4157, 4539
Bme18I GGWCC 5 cut(s) 1048, 1280, 2703, 4834, 5314
BmeRI GACNNNNNGTC 1 cut(s) 4486
BmeT110I CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
BmgBI CACGTC 3 cut(s) 818, 1577, 1835
BmgT120I GGNCC 9 cut(s) 1048, 1280, 2703, 2737, 3108, 3109, 3347, 4834, 5314
BmrFI CCNGG 8 cut(s) 230, 894, 1296, 2953, 3275, 3576, 4157, 4539
BmrI ACTGGG 4 cut(s) 449, 735, 3727, 5715
BmtI GCTAGC 1 cut(s) 5522
BmuI ACTGGG 4 cut(s) 449, 735, 3727, 5715
BpiI GAAGAC 3 cut(s) 3405, 3821, 4692
BplI GAGNNNNNCTC 4 cut(s) 4478, 4510, 4990, 5022
BpmI CTGGAG 7 cut(s) 3973, 4373, 4763, 4944, 5153, 5550, 5805
Bpu10I CCTNAGC 1 cut(s) 234
Bpu1102I GCTNAGC 1 cut(s) 2712
Bpu14I TTCGAA 2 cut(s) 1951, 2908
BpuEI CTTGAG 4 cut(s) 2555, 2580, 3068, 4746
BpuMI CCSGG 2 cut(s) 1296, 4157
Bsa29I ATCGAT 1 cut(s) 3060
BsaAI YACGTR 1 cut(s) 455
BsaBI GATNNNNATC 2 cut(s) 2415, 5016
BsaHI GRCGYC 2 cut(s) 3402, 5479
BsaI GGTCTC 2 cut(s) 2816, 5201
BsaJI CCNNGG 8 cut(s) 893, 1610, 1729, 2658, 3343, 3850, 4622, 4987
BsaWI WCCGGW 3 cut(s) 1686, 4561, 5008
BsaXI ACNNNNNCTCC 8 cut(s) 555, 585, 657, 687, 3387, 3417, 4636, 4666
Bse118I RCCGGY 1 cut(s) 5560
Bse3DI GCAATG 6 cut(s) 483, 615, 3450, 3525, 5355, 5520
Bse8I GATNNNNATC 2 cut(s) 2415, 5016
BseAI TCCGGA 2 cut(s) 1686, 5008
BseBI CCWGG 6 cut(s) 230, 894, 2953, 3275, 3576, 4539
BseCI ATCGAT 1 cut(s) 3060
BseDI CCNNGG 8 cut(s) 893, 1610, 1729, 2658, 3343, 3850, 4622, 4987
BseJI GATNNNNATC 2 cut(s) 2415, 5016
BseMI GCAATG 6 cut(s) 483, 615, 3450, 3525, 5355, 5520
BseMII CTCAG 6 cut(s) 248, 262, 2015, 2703, 4424, 5298
BsePI GCGCGC 1 cut(s) 5665
BseRI GAGGAG 3 cut(s) 3065, 5152, 5526
BseSI GKGCMC 2 cut(s) 2167, 3112
BseYI CCCAGC 4 cut(s) 314, 1988, 2502, 2707
BsgI GTGCAG 7 cut(s) 143, 423, 1637, 1958, 2064, 5048, 5213
Bsh1236I CGCG 4 cut(s) 294, 2934, 5295, 5667
BshVI ATCGAT 1 cut(s) 3060
BsiHKAI GWGCWC 8 cut(s) 725, 853, 2329, 2395, 3196, 3453, 3989, 5602
BsiHKCI CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
BsiSI CCGG 6 cut(s) 1296, 1687, 4156, 4562, 5009, 5561
BslFI GGGAC 7 cut(s) 1061, 1266, 1321, 4692, 4820, 5282, 5300
BsmBI CGTCTC 1 cut(s) 5486
BsmFI GGGAC 7 cut(s) 1061, 1266, 1321, 4692, 4820, 5282, 5300
BsmI GAATGC 1 cut(s) 5340
Bso31I GGTCTC 2 cut(s) 2816, 5201
BsoBI CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
Bsp119I TTCGAA 2 cut(s) 1951, 2908
Bsp120I GGGCCC 1 cut(s) 3108
Bsp13I TCCGGA 2 cut(s) 1686, 5008
Bsp1407I TGTACA 2 cut(s) 715, 3068
Bsp1720I GCTNAGC 1 cut(s) 2712
Bsp19I CCATGG 2 cut(s) 3343, 3850
Bsp68I TCGCGA 2 cut(s) 294, 5295
BspACI CCGC 8 cut(s) 364, 668, 1135, 1530, 2128, 3136, 5233, 5730
BspCNI CTCAG 6 cut(s) 247, 261, 2014, 2704, 4423, 5299
BspDI ATCGAT 1 cut(s) 3060
BspEI TCCGGA 2 cut(s) 1686, 5008
BspFNI CGCG 4 cut(s) 294, 2934, 5295, 5667
BspHI TCATGA 4 cut(s) 3325, 4342, 5169, 5458
BspMAI CTGCAG 2 cut(s) 2482, 3435
BspMI ACCTGC 1 cut(s) 1609
BspOI GCTAGC 1 cut(s) 5522
BspPI GGATC 9 cut(s) 591, 851, 1093, 2787, 3305, 4131, 4428, 4779, 5456
BspQI GCTCTTC 2 cut(s) 363, 2667
BspT104I TTCGAA 2 cut(s) 1951, 2908
BspTNI GGTCTC 2 cut(s) 2816, 5201
BsrBI CCGCTC 3 cut(s) 668, 1532, 3136
BsrDI GCAATG 6 cut(s) 483, 615, 3450, 3525, 5355, 5520
BsrFI RCCGGY 1 cut(s) 5560
BsrGI TGTACA 2 cut(s) 715, 3068
BssAI RCCGGY 1 cut(s) 5560
BssECI CCNNGG 8 cut(s) 893, 1610, 1729, 2658, 3343, 3850, 4622, 4987
BssHII GCGCGC 1 cut(s) 5665
BssNI GRCGYC 2 cut(s) 3402, 5479
BssSI CACGAG 1 cut(s) 5601
BssT1I CCWWGG 6 cut(s) 1729, 2658, 3343, 3850, 4622, 4987
Bst2BI CACGAG 1 cut(s) 5601
Bst2UI CCWGG 6 cut(s) 230, 894, 2953, 3275, 3576, 4539
Bst6I CTCTTC 2 cut(s) 363, 2667
BstACI GRCGYC 2 cut(s) 3402, 5479
BstAPI GCANNNNNTGC 6 cut(s) 260, 889, 2102, 2756, 3725, 5410
BstAUI TGTACA 2 cut(s) 715, 3068
BstBAI YACGTR 1 cut(s) 455
BstBI TTCGAA 2 cut(s) 1951, 2908
BstDSI CCRYGG 2 cut(s) 3343, 3850
BstEII GGTNACC 1 cut(s) 5183
BstENI CCTNNNNNAGG 5 cut(s) 233, 1688, 1733, 2095, 2690
BstFNI CGCG 4 cut(s) 294, 2934, 5295, 5667
BstHHI GCGC 6 cut(s) 254, 1708, 2007, 4695, 5667, 5669
BstNI CCWGG 6 cut(s) 230, 894, 2953, 3275, 3576, 4539
BstNSI RCATGY 4 cut(s) 1143, 3985, 4246, 5759
BstPI GGTNACC 1 cut(s) 5183
BstSCI CCNGG 8 cut(s) 228, 892, 1294, 2951, 3273, 3574, 4155, 4537
BstSFI CTRYAG 6 cut(s) 693, 2304, 2478, 3431, 3502, 5236
BstSLI GKGCMC 2 cut(s) 2167, 3112
BstSNI TACGTA 1 cut(s) 455
BstUI CGCG 4 cut(s) 294, 2934, 5295, 5667
BstV2I GAAGAC 3 cut(s) 3405, 3821, 4692
BstX2I RGATCY 6 cut(s) 583, 856, 3281, 4420, 4771, 5247
BstXI CCANNNNNNTGG 1 cut(s) 2407
BstYI RGATCY 6 cut(s) 583, 856, 3281, 4420, 4771, 5247
Bsu15I ATCGAT 1 cut(s) 3060
BsuI GTATCC 2 cut(s) 1397, 3132
BsuTUI ATCGAT 1 cut(s) 3060
BtgI CCRYGG 2 cut(s) 3343, 3850
BtgZI GCGATG 3 cut(s) 1769, 2569, 2659
BtrI CACGTC 3 cut(s) 818, 1577, 1835
BtsI GCAGTG 5 cut(s) 1983, 2255, 2580, 2847, 3192
BtuMI TCGCGA 2 cut(s) 294, 5295
BveI ACCTGC 1 cut(s) 1609
CaiI CAGNNNCTG 2 cut(s) 164, 3755
CciI TCATGA 4 cut(s) 3325, 4342, 5169, 5458
CfoI GCGC 6 cut(s) 254, 1708, 2007, 4695, 5667, 5669
Cfr10I RCCGGY 1 cut(s) 5560
Cfr13I GGNCC 9 cut(s) 1048, 1280, 2703, 2737, 3108, 3109, 3347, 4834, 5314
ClaI ATCGAT 1 cut(s) 3060
CseI GACGC 3 cut(s) 1190, 1412, 5468
CspCI CAANNNNNGTGG 4 cut(s) 291, 326, 501, 536
DraI TTTAAA 1 cut(s) 270
DriI GACNNNNNGTC 1 cut(s) 4486
EaeI YGGCCR 4 cut(s) 977, 2787, 5436, 5845
Eam1104I CTCTTC 2 cut(s) 363, 2667
Eam1105I GACNNNNNGTC 1 cut(s) 4486
EarI CTCTTC 2 cut(s) 363, 2667
Ecl136II GAGCTC 1 cut(s) 3451
Eco105I TACGTA 1 cut(s) 455
Eco130I CCWWGG 6 cut(s) 1729, 2658, 3343, 3850, 4622, 4987
Eco147I AGGCCT 4 cut(s) 2360, 2576, 4334, 5450
Eco24I GRGCYC 3 cut(s) 3112, 3453, 3568
Eco31I GGTCTC 2 cut(s) 2816, 5201
Eco47I GGWCC 5 cut(s) 1048, 1280, 2703, 4834, 5314
Eco53kI GAGCTC 1 cut(s) 3451
Eco88I CYCGRG 4 cut(s) 1611, 3314, 4277, 5793
Eco91I GGTNACC 1 cut(s) 5183
EcoICRI GAGCTC 1 cut(s) 3451
EcoNI CCTNNNNNAGG 5 cut(s) 233, 1688, 1733, 2095, 2690
EcoO109I RGGNCCY 4 cut(s) 1048, 3109, 4834, 5314
EcoO65I GGTNACC 1 cut(s) 5183
EcoRI GAATTC 1 cut(s) 4747
EcoRII CCWGG 6 cut(s) 228, 892, 2951, 3273, 3574, 4537
EcoT14I CCWWGG 6 cut(s) 1729, 2658, 3343, 3850, 4622, 4987
EcoT22I ATGCAT 1 cut(s) 1518
EcoT38I GRGCYC 3 cut(s) 3112, 3453, 3568
ErhI CCWWGG 6 cut(s) 1729, 2658, 3343, 3850, 4622, 4987
Esp3I CGTCTC 1 cut(s) 5486
FaqI GGGAC 7 cut(s) 1061, 1266, 1321, 4692, 4820, 5282, 5300
FauI CCCGC 2 cut(s) 1537, 2121
FauNDI CATATG 1 cut(s) 1512
FbaI TGATCA 2 cut(s) 5017, 5803
FblI GTMKAC 3 cut(s) 809, 1864, 5763
FriOI GRGCYC 3 cut(s) 3112, 3453, 3568
GlaI GCGC 6 cut(s) 253, 1707, 2006, 4694, 5666, 5668
GsaI CCCAGC 4 cut(s) 318, 1992, 2506, 2711
GsuI CTGGAG 7 cut(s) 3973, 4373, 4763, 4944, 5153, 5550, 5805
HapII CCGG 6 cut(s) 1296, 1687, 4156, 4562, 5009, 5561
HgaI GACGC 3 cut(s) 1190, 1412, 5468
HhaI GCGC 6 cut(s) 254, 1708, 2007, 4695, 5667, 5669
Hin1I GRCGYC 2 cut(s) 3402, 5479
Hin6I GCGC 6 cut(s) 252, 1706, 2005, 4693, 5665, 5667
HinP1I GCGC 6 cut(s) 252, 1706, 2005, 4693, 5665, 5667
HincII GTYRAC 6 cut(s) 384, 938, 1260, 1865, 2609, 4090
HindII GTYRAC 6 cut(s) 384, 938, 1260, 1865, 2609, 4090
HindIII AAGCTT 6 cut(s) 137, 651, 886, 1175, 5280, 5699
HpaII CCGG 6 cut(s) 1296, 1687, 4156, 4562, 5009, 5561
Hpy99I CGWCG 3 cut(s) 1836, 1869, 3108
Hsp92I GRCGYC 2 cut(s) 3402, 5479
HspAI GCGC 6 cut(s) 252, 1706, 2005, 4693, 5665, 5667
KflI GGGWCCC 2 cut(s) 1048, 4834
Kpn2I TCCGGA 2 cut(s) 1686, 5008
Ksp22I TGATCA 2 cut(s) 5017, 5803
LguI GCTCTTC 2 cut(s) 363, 2667
MbiI CCGCTC 3 cut(s) 668, 1532, 3136
MfeI CAATTG 1 cut(s) 3486
MflI RGATCY 6 cut(s) 583, 856, 3281, 4420, 4771, 5247
MlsI TGGCCA 3 cut(s) 979, 5438, 5847
MluNI TGGCCA 3 cut(s) 979, 5438, 5847
MlyI GAGTC 2 cut(s) 2614, 4496
MmeI TCCRAC 3 cut(s) 1063, 2464, 3233
Mox20I TGGCCA 3 cut(s) 979, 5438, 5847
Mph1103I ATGCAT 1 cut(s) 1518
MroI TCCGGA 2 cut(s) 1686, 5008
MroXI GAANNNNTTC 3 cut(s) 138, 5301, 5616
MscI TGGCCA 3 cut(s) 979, 5438, 5847
MslI CAYNNNNRTG 3 cut(s) 906, 4644, 5174
Msp20I TGGCCA 3 cut(s) 979, 5438, 5847
MspA1I CMGCKG 5 cut(s) 2144, 2711, 3686, 4459, 5732
MspI CCGG 6 cut(s) 1296, 1687, 4156, 4562, 5009, 5561
MspR9I CCNGG 8 cut(s) 230, 894, 1296, 2953, 3275, 3576, 4157, 4539
MunI CAATTG 1 cut(s) 3486
Mva1269I GAATGC 1 cut(s) 5340
MvaI CCWGG 6 cut(s) 230, 894, 2953, 3275, 3576, 4539
MvnI CGCG 4 cut(s) 294, 2934, 5295, 5667
NciI CCSGG 2 cut(s) 1296, 4157
NcoI CCATGG 2 cut(s) 3343, 3850
NdeI CATATG 1 cut(s) 1512
NheI GCTAGC 1 cut(s) 5518
NmeAIII GCCGAG 1 cut(s) 4426
NruI TCGCGA 2 cut(s) 294, 5295
NsiI ATGCAT 1 cut(s) 1518
NspI RCATGY 4 cut(s) 1143, 3985, 4246, 5759
NspV TTCGAA 2 cut(s) 1951, 2908
PaeI GCATGC 1 cut(s) 4246
PaeR7I CTCGAG 2 cut(s) 4277, 5793
PagI TCATGA 4 cut(s) 3325, 4342, 5169, 5458
PauI GCGCGC 1 cut(s) 5665
PceI AGGCCT 4 cut(s) 2360, 2576, 4334, 5450
PciI ACATGT 1 cut(s) 1139
PciSI GCTCTTC 2 cut(s) 363, 2667
PcsI WCGNNNNNNNCGW 1 cut(s) 5599
PctI GAATGC 1 cut(s) 5340
PdmI GAANNNNTTC 3 cut(s) 138, 5301, 5616
PfeI GAWTC 9 cut(s) 38, 929, 2066, 2531, 3085, 3473, 3590, 4556, 5290
PflFI GACNNNGTC 2 cut(s) 1697, 3913
PflMI CCANNNNNTGG 3 cut(s) 952, 2123, 4817
PfoI TCCNGGA 1 cut(s) 3574
PleI GAGTC 2 cut(s) 2613, 4495
PpsI GAGTC 2 cut(s) 2613, 4495
Ppu21I YACGTR 1 cut(s) 455
PpuMI RGGWCCY 3 cut(s) 1048, 4834, 5314
PscI ACATGT 1 cut(s) 1139
PsiI TTATAA 1 cut(s) 1166
Psp124BI GAGCTC 1 cut(s) 3453
Psp1406I AACGTT 1 cut(s) 3159
Psp5II RGGWCCY 3 cut(s) 1048, 4834, 5314
Psp6I CCWGG 6 cut(s) 228, 892, 2951, 3273, 3574, 4537
PspEI GGTNACC 1 cut(s) 5183
PspFI CCCAGC 4 cut(s) 314, 1988, 2502, 2707
PspGI CCWGG 6 cut(s) 228, 892, 2951, 3273, 3574, 4537
PspOMI GGGCCC 1 cut(s) 3108
PspPI GGNCC 9 cut(s) 1048, 1280, 2703, 2737, 3108, 3109, 3347, 4834, 5314
PspPPI RGGWCCY 3 cut(s) 1048, 4834, 5314
PspXI VCTCGAGB 1 cut(s) 4277
PsrI GAACNNNNNNTAC 2 cut(s) 816, 848
PstI CTGCAG 2 cut(s) 2482, 3435
PstNI CAGNNNCTG 2 cut(s) 164, 3755
PsuI RGATCY 6 cut(s) 583, 856, 3281, 4420, 4771, 5247
PsyI GACNNNGTC 2 cut(s) 1697, 3913
PteI GCGCGC 1 cut(s) 5665
PvuII CAGCTG 4 cut(s) 2144, 2711, 3686, 4459
RruI TCGCGA 2 cut(s) 294, 5295
RseI CAYNNNNRTG 3 cut(s) 906, 4644, 5174
SacI GAGCTC 1 cut(s) 3453
SalI GTCGAC 1 cut(s) 1863
SapI GCTCTTC 2 cut(s) 363, 2667
Sau96I GGNCC 9 cut(s) 1048, 1280, 2703, 2737, 3108, 3109, 3347, 4834, 5314
ScaI AGTACT 2 cut(s) 825, 3508
SchI GAGTC 2 cut(s) 2614, 4496
ScrFI CCNGG 8 cut(s) 230, 894, 1296, 2953, 3275, 3576, 4157, 4539
SfcI CTRYAG 6 cut(s) 693, 2304, 2478, 3431, 3502, 5236
Sfr274I CTCGAG 2 cut(s) 4277, 5793
SfuI TTCGAA 2 cut(s) 1951, 2908
SinI GGWCC 5 cut(s) 1048, 1280, 2703, 4834, 5314
SlaI CTCGAG 2 cut(s) 4277, 5793
SmiMI CAYNNNNRTG 3 cut(s) 906, 4644, 5174
SmlI CTYRAG 6 cut(s) 2570, 2595, 3047, 4277, 4761, 5793
SmoI CTYRAG 6 cut(s) 2570, 2595, 3047, 4277, 4761, 5793
SnaBI TACGTA 1 cut(s) 455
SpeI ACTAGT 1 cut(s) 2180
SphI GCATGC 1 cut(s) 4246
SseBI AGGCCT 4 cut(s) 2360, 2576, 4334, 5450
SsiI CCGC 8 cut(s) 364, 668, 1135, 1530, 2128, 3136, 5233, 5730
SspI AATATT 3 cut(s) 189, 2233, 2490
SstI GAGCTC 1 cut(s) 3453
StuI AGGCCT 4 cut(s) 2360, 2576, 4334, 5450
StyD4I CCNGG 8 cut(s) 228, 892, 1294, 2951, 3273, 3574, 4155, 4537
StyI CCWWGG 6 cut(s) 1729, 2658, 3343, 3850, 4622, 4987
TatI WGTACW 6 cut(s) 715, 823, 3068, 3506, 4872, 4924
TauI GCSGC 2 cut(s) 366, 671
TfiI GAWTC 9 cut(s) 38, 929, 2066, 2531, 3085, 3473, 3590, 4556, 5290
TspGWI ACGGA 7 cut(s) 84, 468, 732, 1228, 1529, 4160, 5369
Tth111I GACNNNGTC 2 cut(s) 1697, 3913
Van91I CCANNNNNTGG 3 cut(s) 952, 2123, 4817
VpaK11BI GGWCC 5 cut(s) 1048, 1280, 2703, 4834, 5314
XagI CCTNNNNNAGG 5 cut(s) 233, 1688, 1733, 2095, 2690
XapI RAATTY 8 cut(s) 26, 2244, 2381, 2509, 3436, 3603, 4747, 5866
XbaI TCTAGA 2 cut(s) 853, 4751
XceI RCATGY 4 cut(s) 1143, 3985, 4246, 5759
XcmI CCANNNNNNNNNTGG 3 cut(s) 2959, 3389, 5591
XhoI CTCGAG 2 cut(s) 4277, 5793
XmiI GTMKAC 3 cut(s) 809, 1864, 5763
XmnI GAANNNNTTC 3 cut(s) 138, 5301, 5616
ZraI GACGTC 1 cut(s) 3403
ZrmI AGTACT 2 cut(s) 825, 3508
Zsp2I ATGCAT 1 cut(s) 1518
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.