Rw0G021390

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00986
OG0005708
Physical Location & Seq
Reverse (-)
876 .. 13890
13015 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G021390.1

Sequence Viewer

Length: 3822 bp
ATGGCCGTCGCGGTGCAGTCCCTCAGGTTCGGCGGGCCCGTCACCGCCGCACCCGGCAGCGGAAGCTTCGACGCCCTCAATCGTATCCTCGCCGACCTCTGCACGCGTGGCAACCCCAAGGAGGGGGCTTCGTTGGCTTTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACTTAAGTGGAGAAGCATTTTCTCGTTTCATGGATCAGTTGTATGATCGGATTTCTGCTCTTTTGGATAGCAGTGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGATGAGTTGATAGATGTGGCACTTGGAGAGAATGCATCAAAGGTTTCAAAGTTTGCTAATTACATAAGGACTGCCTTTGAGCTAAAACGTGATCCTGACATTTTGGTCCTGGCTAGTAGAGTTTTGGGCCATCTAGCTAGGGCCGGGGGAGCAATGACTGCAGATGAAGTGGAATGCCAGATAAAAATTGCTCTGGAATGGTTGCGTGGAGACAGAATTGAGTATCGACGTTTTGCTGCCGTCTTGATTTTGAAAGAGATGGCAGAAAATGCTTCCACAGTGTTCAATGTTCATGTACCTGAGTTTGTTGATGCTATCTGGGTTGCTTTGAGAGATCCAGTATTGGCTATCCGAGAGCGAGCTGTGGAAGCTCTGCGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGATGGCGTGTGCAATGGTATTATCGTATGTTCGAAGCTACACAGGATGGATTGGGTAAAAATGCTTCTGTTCACAGCATACATGGTTCTTTACTTGCAGTTGGAGAGCTTTTAAGGAATACAGGTGAATTCATGATGTCAAGGTATAGGGAAGTTGCAGAAATTGTCCTTAGGTATCTTGACCACAAGGATAGGCTGGTTCGCCTGAGCATAACTTCACTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTTACAAACTACTTAGAGACATGTATGAATCATATCCTCGCTGTTTTGCGACAATCAGCTGAACTACGCTCCAGTGGTTTTATTGCACTTGGGGAGATGGCTGGTGCTTTGGACGGGGAACTGGTTTTCTATTTGGTCCAAATTACACCTCACTTACGTGAAGCAATTGCTCCACGTAGAGGGCGACCCTCACTTGAGGCTTTGGCTTGTGTTGGAAATATTGCAAAAGCAATGGGGCCTGCTATGGAACATGATGTTCGTGGTCTTTTGGATGTTATGTTCGCTGCTGGTCTTTCCTCTACGCTTGTTGAGGCCCTTGAAAAGATAACTGCCAGTATACCATCTTTGCTGCCTACCATCCAAGATCGGTTGCTAGAATGCATTTCAGTGGTTCTTTCTAAATCCCAACATCCTCAGGGACGGCCAGTTGTTGGAATGGGTAGAGGAAATTTAATAAATATCCCTCAGCAAGTTTCAGACCTCAGTGGCTCATCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTCAATTTCAAGGGTCACGACCTTCTTGAATTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGACGGAGCCATCCGAAAAGATGCTGCCCTTTGTTGTTGCAGATTGGTTGCAAATTCCTTTTCTGGTGTACAATATGCTTCTGGTAGGTCAAATCGGGGAAAGCGACGCCGTCTTGTCGAGGAGATTGTTGAAAAGCTTCTCATTGAGGCTGTTGCAGATGCTGATGTGATTGTTCGCCATTCAATATTTTCTTCTCTACATGGGAATAGAGGCTTTGACGAATTCCTGGCTCAGGCTGATAGTCTCAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGCGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCTTATGTTCTTCCAGCTCTTCGCCGCCATCTTATACAGTTGTTGACTTACTTGGGTCAGAGTGCAGATAGCAAATGCAGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGTAACTGTGAAAGACTAATACTTCCATACATTGCTCCTATACACAAGGCACTTGTGGCAAGACTTATGGATGGCACTGGTGTCGGCTCGAATAATGGCATCATTAGTGGAGTTCTTGTAACTGTTGGGGATCTTGCTAGAGTGGGTGGGTTTGCAATGAGAAAATATATACCAGAGCTTATGCCTTTGATTGTTGATGCTTTATTGGATGGAGCGGCTGTCACAAAACGGGAAGTGGCTGTAGCTACTCTCGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCGTATAATGAATATCCACTGTTGCTTGGCTTACTCTTGAAGTTGCTTAACGGTGAGTTGGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTTGGAATTATGGGTGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCTTGCCTGGGTCACATGGAGATGTTCCTCGTAATGCCAGTGAGTCTGGTCAACACATCCAATCTGTGGATGAATTGCCTATGGACTTGTGGCCATCATTTGCAACCTCTGAAGATTATTATTCTACGGTTGCAATCAATTCTCTCATGCGAATACTTAGGGACCCTTCACTTGGTACTTACCACCTAAAGGTTGTTGGATCACTTATGTTTATATTCAAGTCAATGGGTCTCGGTTGTGTTCCGTACTTACCAAAGGTGTTGCCTGACCTTTTCCATATAGTCCGCACATGTGATGATGCTTTGAAGGATTTTATAACATGGAAGCTTGGAACTCTAGTGTCTATTGTGCGTCAGCACATACGGAAATATCTGCATGAATTGTTGGCTCTAATTTCAGAACTATGGTCAACCTTTAGTTTCCCTGCTGGTGTCCGTCCTCAACCTGGCTATCCAGTTCTTCATCTGGTGGAGCAGCTTTGTTTGGCCCTGAATGACGAGTTTAGGACGTATCTTCCTGATATTCTTCCATGCTGTATCCAAGTTCTAAGTGACGCAGAACGGTGTAATGACTACACTTACGTTCTTGACATCCTCCATACCCTTGAAGTATTTAGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCCGCTCTAATCCGTTTGTTCAAAGTGGATGCTTCAGTGGATATAAGACGTGCTGCTATCAAAACTTTGACCAAACTCATCCCTCGGGTGCAGGTTACTGGTCACATATCTTCTCTTGTGCATCACTTGAAGCTAGTCTTAGATGGGAAGAATGATGACCTCCGGAAGGACACTGTCGATGCTCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGATTTCACCATCTTTATCCCATCCATACACAAACTTTTGTTAAAACATCGCTTACGGCACAAGGGGTTTGAGGAAATTGAAGGCCATTTGCATCGACGTGAACCTTTTGCTCCCCAAAGATTAAGTCGACGACTTCCAGAGGTTGTTGCTGACCGTTCAACTGATTTGGAGATTGACCCTTATGATGATGTGACTGATGTGCAGAAAAAACTTCGAAGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACACTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCACTACGAACCTGTGCAAGGCTTGCACAGTTCCAGCCATTTGTTGGGCGGGAGTTGTTTGCAGCTGGATTTGTTAGCTGTTGGGCACAGCTAAACGAGACTAGTCAGAAACAGTTAGTTCGGAGTTTGGAGATGGCATTTTCATCTCCAAATATTCCTCCTGAAATTTTGGCAACACTGCTTAATCTG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

1274

Amino Acids

141.68

Weight (kDa)

6.59

Isoelectric Point (pI)

42.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 759 - 927 3.7e-52 Serine/threonine-protein kinase mTOR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2744
AasI GACNNNNNNGTC 1 cut(s) 383
Acc36I ACCTGC 1 cut(s) 3160
AccB7I CCANNNNNTGG 3 cut(s) 1379, 2494, 3677
AccBSI CCGCTC 2 cut(s) 2210, 3083
AccI GTMKAC 3 cut(s) 1285, 2351, 3418
AccII CGCG 3 cut(s) 11, 106, 1836
AccIII TCCGGA 1 cut(s) 3240
AclWI GGATC 6 cut(s) 210, 365, 608, 2133, 2393, 2635
AcoI YGGCCR 3 cut(s) 3, 1370, 2519
AcsI RAATTY 7 cut(s) 256, 818, 1396, 1505, 1597, 1766, 3798
AcuI CTGAAG 3 cut(s) 2559, 3096, 3635
AcyI GRCGYC 2 cut(s) 72, 1651
AfaI GTAC 7 cut(s) 576, 1614, 2259, 2367, 2605, 2675, 3565
AflII CTTAAG 1 cut(s) 172
AflIII ACRYGT 3 cut(s) 104, 968, 2717
AhlI ACTAGT 1 cut(s) 3734
AjiI CACGTC 3 cut(s) 2360, 3128, 3389
AjnI CCWGG 4 cut(s) 387, 1770, 2434, 2872
AleI CACNNNNGTG 1 cut(s) 1104
AloI GAACNNNNNNTCC 2 cut(s) 1211, 1243
Alw21I GWGCWC 3 cut(s) 268, 2267, 2395
Alw26I GTCTC 7 cut(s) 483, 680, 959, 1793, 2663, 3586, 3725
AlwI GGATC 6 cut(s) 210, 365, 608, 2133, 2393, 2635
AlwNI CAGNNNCTG 1 cut(s) 1706
Ama87I CYCGRG 1 cut(s) 3162
Aor13HI TCCGGA 1 cut(s) 3240
ApaI GGGCCC 1 cut(s) 39
ApoI RAATTY 7 cut(s) 256, 818, 1396, 1505, 1597, 1766, 3798
ArsI GACNNNNNNTTYG 2 cut(s) 3400, 3432
Asp700I GAANNNNTTC 1 cut(s) 1680
AspLEI GCGC 2 cut(s) 1796, 3561
AsuC2I CCSGG 2 cut(s) 54, 424
AsuHPI GGTGA 4 cut(s) 34, 827, 2348, 3289
AsuII TTCGAA 2 cut(s) 723, 3505
AvaI CYCGRG 1 cut(s) 3162
AvaII GGWCC 3 cut(s) 385, 1084, 2590
AxyI CCTNAGG 3 cut(s) 23, 860, 1362
BaeGI GKGCMC 2 cut(s) 39, 3721
BalI TGGCCA 1 cut(s) 2521
BanII GRGCYC 2 cut(s) 39, 268
BarI GAAGNNNNNNTAC 2 cut(s) 739, 771
Bbv12I GWGCWC 3 cut(s) 268, 2267, 2395
BbvCI CCTCAGC 1 cut(s) 1413
BceAI ACGGC 4 cut(s) 503, 1385, 1638, 3362
BcgI CGANNNNNNTGC 2 cut(s) 2059, 2093
BciT130I CCWGG 4 cut(s) 389, 1772, 2436, 2874
BciVI GTATCC 2 cut(s) 95, 2975
BcnI CCSGG 2 cut(s) 54, 424
BcoDI GTCTC 7 cut(s) 483, 680, 959, 1793, 2663, 3586, 3725
BcuI ACTAGT 1 cut(s) 3734
BfmI CTRYAG 2 cut(s) 438, 2235
BfrI CTTAAG 1 cut(s) 172
BfuAI ACCTGC 1 cut(s) 3160
BfuI GTATCC 2 cut(s) 95, 2975
BmcAI AGTACT 1 cut(s) 2367
Bme1390I CCNGG 6 cut(s) 54, 389, 424, 1772, 2436, 2874
Bme18I GGWCC 3 cut(s) 385, 1084, 2590
BmeT110I CYCGRG 1 cut(s) 3162
BmgBI CACGTC 3 cut(s) 2360, 3128, 3389
BmiI GGNNCC 5 cut(s) 37, 1185, 1552, 2591, 2592
BmrFI CCNGG 6 cut(s) 54, 389, 424, 1772, 2436, 2874
BmrI ACTGGG 2 cut(s) 1991, 2277
BmuI ACTGGG 2 cut(s) 1991, 2277
BpmI CTGGAG 1 cut(s) 1003
Bpu10I CCTNAGC 3 cut(s) 896, 1413, 1776
Bpu14I TTCGAA 2 cut(s) 723, 3505
BpuEI CTTGAG 1 cut(s) 1163
BpuMI CCSGG 2 cut(s) 54, 424
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 3 cut(s) 1106, 1124, 1997
BsaHI GRCGYC 2 cut(s) 72, 1651
BsaI GGTCTC 1 cut(s) 2663
BsaJI CCNNGG 6 cut(s) 117, 423, 664, 2435, 3161, 3283
BsaWI WCCGGW 1 cut(s) 3240
BsaXI ACNNNNNCTCC 4 cut(s) 2097, 2127, 2199, 2229
Bse21I CCTNAGG 3 cut(s) 23, 860, 1362
Bse3DI GCAATG 5 cut(s) 438, 710, 1185, 2025, 2157
BseAI TCCGGA 1 cut(s) 3240
BseBI CCWGG 4 cut(s) 389, 1772, 2436, 2874
BseCI ATCGAT 1 cut(s) 943
BseDI CCNNGG 6 cut(s) 117, 423, 664, 2435, 3161, 3283
BseMI GCAATG 5 cut(s) 438, 710, 1185, 2025, 2157
BseMII CTCAG 9 cut(s) 37, 570, 887, 1376, 1427, 1444, 1790, 1804, 3569
BseRI GAGGAG 1 cut(s) 1679
BseSI GKGCMC 2 cut(s) 39, 3721
BseYI CCCAGC 2 cut(s) 1856, 3542
BsgI GTGCAG 7 cut(s) 35, 85, 1469, 1965, 3188, 3512, 3618
Bsh1236I CGCG 3 cut(s) 11, 106, 1836
BshVI ATCGAT 1 cut(s) 943
BsiHKAI GWGCWC 3 cut(s) 268, 2267, 2395
BsiHKCI CYCGRG 1 cut(s) 3162
BsiSI CCGG 3 cut(s) 54, 423, 3241
BslFI GGGAC 3 cut(s) 4, 1380, 2603
BsmAI GTCTC 7 cut(s) 483, 680, 959, 1793, 2663, 3586, 3725
BsmFI GGGAC 3 cut(s) 4, 1380, 2603
BsmI GAATGC 3 cut(s) 316, 458, 1331
Bso31I GGTCTC 1 cut(s) 2663
BsoBI CYCGRG 1 cut(s) 3162
Bsp119I TTCGAA 2 cut(s) 723, 3505
Bsp120I GGGCCC 1 cut(s) 35
Bsp1286I GDGCHC 5 cut(s) 39, 268, 2267, 2395, 3721
Bsp13I TCCGGA 1 cut(s) 3240
Bsp1407I TGTACA 2 cut(s) 1612, 2257
Bsp143I GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
Bsp68I TCGCGA 1 cut(s) 1836
BspCNI CTCAG 9 cut(s) 36, 571, 888, 1375, 1426, 1443, 1789, 1803, 3568
BspDI ATCGAT 1 cut(s) 943
BspEI TCCGGA 1 cut(s) 3240
BspFNI CGCG 3 cut(s) 11, 106, 1836
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 5 cut(s) 37, 1185, 1552, 2591, 2592
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 1 cut(s) 3160
BspPI GGATC 6 cut(s) 210, 365, 608, 2133, 2393, 2635
BspQI GCTCTTC 1 cut(s) 1905
BspT104I TTCGAA 2 cut(s) 723, 3505
BspTI CTTAAG 1 cut(s) 172
BspTNI GGTCTC 1 cut(s) 2663
BsrBI CCGCTC 2 cut(s) 2210, 3083
BsrDI GCAATG 5 cut(s) 438, 710, 1185, 2025, 2157
BsrGI TGTACA 2 cut(s) 1612, 2257
BssECI CCNNGG 6 cut(s) 117, 423, 664, 2435, 3161, 3283
BssMI GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
BssNAI GTATAC 1 cut(s) 1286
BssNI GRCGYC 2 cut(s) 72, 1651
BssT1I CCWWGG 3 cut(s) 117, 664, 3283
Bst1107I GTATAC 1 cut(s) 1286
Bst2UI CCWGG 4 cut(s) 389, 1772, 2436, 2874
Bst6I CTCTTC 1 cut(s) 1905
BstACI GRCGYC 2 cut(s) 72, 1651
BstAFI CTTAAG 1 cut(s) 172
BstAPI GCANNNNNTGC 5 cut(s) 437, 548, 1802, 2431, 3656
BstAUI TGTACA 2 cut(s) 1612, 2257
BstBAI YACGTR 3 cut(s) 1106, 1124, 1997
BstBI TTCGAA 2 cut(s) 723, 3505
BstC8I GCNNGC 9 cut(s) 35, 104, 639, 657, 661, 1188, 1455, 2432, 3657
BstENI CCTNNNNNAGG 4 cut(s) 1775, 3242, 3287, 3649
BstFNI CGCG 3 cut(s) 11, 106, 1836
BstHHI GCGC 2 cut(s) 1796, 3561
BstKTI GATC 9 cut(s) 205, 217, 373, 616, 943, 1315, 2128, 2401, 2630
BstMAI GTCTC 7 cut(s) 483, 680, 959, 1793, 2663, 3586, 3725
BstMBI GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
BstNI CCWGG 4 cut(s) 389, 1772, 2436, 2874
BstNSI RCATGY 2 cut(s) 972, 2721
BstSCI CCNGG 6 cut(s) 52, 387, 422, 1770, 2434, 2872
BstSFI CTRYAG 2 cut(s) 438, 2235
BstSLI GKGCMC 2 cut(s) 39, 3721
BstSNI TACGTA 1 cut(s) 1997
BstUI CGCG 3 cut(s) 11, 106, 1836
BstX2I RGATCY 3 cut(s) 613, 2125, 2398
BstYI RGATCY 3 cut(s) 613, 2125, 2398
BstZ17I GTATAC 1 cut(s) 1286
Bsu15I ATCGAT 1 cut(s) 943
Bsu36I CCTNAGG 3 cut(s) 23, 860, 1362
BsuI GTATCC 2 cut(s) 95, 2975
BsuTUI ATCGAT 1 cut(s) 943
BtgZI GCGATG 1 cut(s) 3323
BtrI CACGTC 3 cut(s) 2360, 3128, 3389
BtsI GCAGTG 4 cut(s) 247, 908, 3537, 3809
BtuMI TCGCGA 1 cut(s) 1836
BveI ACCTGC 1 cut(s) 3160
Cac8I GCNNGC 9 cut(s) 35, 104, 639, 657, 661, 1188, 1455, 2432, 3657
CaiI CAGNNNCTG 1 cut(s) 1706
CciI TCATGA 1 cut(s) 822
CfoI GCGC 2 cut(s) 1796, 3561
ClaI ATCGAT 1 cut(s) 943
CseI GACGC 4 cut(s) 80, 1659, 2768, 2990
Csp6I GTAC 7 cut(s) 575, 1613, 2258, 2366, 2604, 2674, 3564
CspCI CAANNNNNGTGG 2 cut(s) 1833, 1868
CviQI GTAC 7 cut(s) 575, 1613, 2258, 2366, 2604, 2674, 3564
DpnI GATC 9 cut(s) 204, 216, 372, 615, 942, 1314, 2127, 2400, 2629
DpnII GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
DraI TTTAAA 1 cut(s) 1812
DrdI GACNNNNNNGTC 1 cut(s) 383
DseDI GACNNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 3 cut(s) 3, 1370, 2519
Eam1104I CTCTTC 1 cut(s) 1905
EarI CTCTTC 1 cut(s) 1905
Ecl136II GAGCTC 1 cut(s) 266
Eco105I TACGTA 1 cut(s) 1997
Eco130I CCWWGG 3 cut(s) 117, 664, 3283
Eco24I GRGCYC 2 cut(s) 39, 268
Eco31I GGTCTC 1 cut(s) 2663
Eco47I GGWCC 3 cut(s) 385, 1084, 2590
Eco53kI GAGCTC 1 cut(s) 266
Eco57I CTGAAG 3 cut(s) 2559, 3096, 3635
Eco81I CCTNAGG 3 cut(s) 23, 860, 1362
Eco88I CYCGRG 1 cut(s) 3162
EcoICRI GAGCTC 1 cut(s) 266
EcoNI CCTNNNNNAGG 4 cut(s) 1775, 3242, 3287, 3649
EcoO109I RGGNCCY 3 cut(s) 1184, 1261, 2590
EcoRI GAATTC 2 cut(s) 818, 1766
EcoRII CCWGG 4 cut(s) 387, 1770, 2434, 2872
EcoT14I CCWWGG 3 cut(s) 117, 664, 3283
EcoT22I ATGCAT 3 cut(s) 316, 1331, 3069
EcoT38I GRGCYC 2 cut(s) 39, 268
ErhI CCWWGG 3 cut(s) 117, 664, 3283
FalI AAGNNNNNCTT 2 cut(s) 3200, 3232
FaqI GGGAC 3 cut(s) 4, 1380, 2603
FauI CCCGC 3 cut(s) 26, 3088, 3675
FauNDI CATATG 1 cut(s) 3063
FblI GTMKAC 3 cut(s) 1285, 2351, 3418
FriOI GRGCYC 2 cut(s) 39, 268
GlaI GCGC 2 cut(s) 1795, 3560
GsaI CCCAGC 2 cut(s) 1860, 3546
GsuI CTGGAG 1 cut(s) 1003
HapII CCGG 3 cut(s) 54, 423, 3241
HgaI GACGC 4 cut(s) 80, 1659, 2768, 2990
HhaI GCGC 2 cut(s) 1796, 3561
Hin1I GRCGYC 2 cut(s) 72, 1651
Hin6I GCGC 2 cut(s) 1794, 3559
HinP1I GCGC 2 cut(s) 1794, 3559
HincII GTYRAC 4 cut(s) 1926, 2480, 2838, 3419
HindII GTYRAC 4 cut(s) 1926, 2480, 2838, 3419
HindIII AAGCTT 4 cut(s) 64, 1679, 2428, 2753
HinfI GANTC 5 cut(s) 976, 1465, 1517, 2471, 3620
HpaII CCGG 3 cut(s) 54, 423, 3241
HphI GGTGA 4 cut(s) 34, 827, 2348, 3289
Hpy166II GTNNAC 9 cut(s) 763, 1286, 1613, 1926, 2352, 2480, 2838, 3392, 3419
Hpy8I GTNNAC 9 cut(s) 763, 1286, 1613, 1926, 2352, 2480, 2838, 3392, 3419
Hpy99I CGWCG 6 cut(s) 11, 74, 510, 1653, 3390, 3423
HpyAV CCTTC 7 cut(s) 1508, 2604, 2728, 3238, 3365, 3610, 3636
Hsp92I GRCGYC 2 cut(s) 72, 1651
HspAI GCGC 2 cut(s) 1794, 3559
KflI GGGWCCC 1 cut(s) 2590
Kpn2I TCCGGA 1 cut(s) 3240
Kzo9I GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
LguI GCTCTTC 1 cut(s) 1905
LmnI GCTCC 9 cut(s) 263, 428, 1022, 1123, 1550, 2035, 2207, 2899, 3406
MalI GATC 9 cut(s) 204, 216, 372, 615, 942, 1314, 2127, 2400, 2629
MbiI CCGCTC 2 cut(s) 2210, 3083
MboI GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 3 cut(s) 613, 2125, 2398
MhlI GDGCHC 5 cut(s) 39, 268, 2267, 2395, 3721
MlsI TGGCCA 1 cut(s) 2521
MluI ACGCGT 1 cut(s) 104
MluNI TGGCCA 1 cut(s) 2521
MlyI GAGTC 2 cut(s) 1459, 2480
MmeI TCCRAC 4 cut(s) 772, 1141, 1360, 2605
Mox20I TGGCCA 1 cut(s) 2521
Mph1103I ATGCAT 3 cut(s) 316, 1331, 3069
MroI TCCGGA 1 cut(s) 3240
MroXI GAANNNNTTC 1 cut(s) 1680
MscI TGGCCA 1 cut(s) 2521
MseI TTAA 9 cut(s) 173, 803, 1400, 1811, 1991, 2331, 3332, 3413, 3816
MslI CAYNNNNRTG 4 cut(s) 1104, 1334, 2448, 3387
Msp20I TGGCCA 1 cut(s) 2521
MspA1I CMGCKG 3 cut(s) 60, 1007, 3698
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 3 cut(s) 54, 423, 3241
MspR9I CCNGG 6 cut(s) 54, 389, 424, 1772, 2436, 2874
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 3 cut(s) 316, 458, 1331
MvaI CCWGG 4 cut(s) 389, 1772, 2436, 2874
MvnI CGCG 3 cut(s) 11, 106, 1836
NciI CCSGG 2 cut(s) 54, 424
NdeI CATATG 1 cut(s) 3063
NdeII GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
NlaIV GGNNCC 5 cut(s) 37, 1185, 1552, 2591, 2592
NmuCI GTSAC 8 cut(s) 40, 167, 1490, 2215, 2439, 2978, 3179, 3481
NruI TCGCGA 1 cut(s) 1836
NsiI ATGCAT 3 cut(s) 316, 1331, 3069
NspI RCATGY 2 cut(s) 972, 2721
NspV TTCGAA 2 cut(s) 723, 3505
OliI CACNNNNGTG 1 cut(s) 1104
PagI TCATGA 1 cut(s) 822
PciI ACATGT 2 cut(s) 968, 2717
PciSI GCTCTTC 1 cut(s) 1905
PcsI WCGNNNNNNNCGW 4 cut(s) 36, 688, 1129, 1645
PctI GAATGC 3 cut(s) 316, 458, 1331
PdmI GAANNNNTTC 1 cut(s) 1680
PfeI GAWTC 3 cut(s) 976, 1517, 3620
PflFI GACNNNGTC 2 cut(s) 1653, 3251
PflMI CCANNNNNTGG 3 cut(s) 1379, 2494, 3677
PleI GAGTC 2 cut(s) 1459, 2479
PpsI GAGTC 2 cut(s) 1459, 2479
Ppu21I YACGTR 3 cut(s) 1106, 1124, 1997
PpuMI RGGWCCY 1 cut(s) 2590
PscI ACATGT 2 cut(s) 968, 2717
PsiI TTATAA 1 cut(s) 2744
Psp124BI GAGCTC 1 cut(s) 268
Psp5II RGGWCCY 1 cut(s) 2590
Psp6I CCWGG 4 cut(s) 387, 1770, 2434, 2872
PspFI CCCAGC 2 cut(s) 1856, 3542
PspGI CCWGG 4 cut(s) 387, 1770, 2434, 2872
PspN4I GGNNCC 5 cut(s) 37, 1185, 1552, 2591, 2592
PspOMI GGGCCC 1 cut(s) 35
PspPPI RGGWCCY 1 cut(s) 2590
PsrI GAACNNNNNNTAC 2 cut(s) 2358, 2390
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 1 cut(s) 1706
PsuI RGATCY 3 cut(s) 613, 2125, 2398
PsyI GACNNNGTC 2 cut(s) 1653, 3251
PvuII CAGCTG 2 cut(s) 1007, 3698
RruI TCGCGA 1 cut(s) 1836
RsaI GTAC 7 cut(s) 576, 1614, 2259, 2367, 2605, 2675, 3565
RsaNI GTAC 7 cut(s) 575, 1613, 2258, 2366, 2604, 2674, 3564
RseI CAYNNNNRTG 4 cut(s) 1104, 1334, 2448, 3387
SacI GAGCTC 1 cut(s) 268
SalI GTCGAC 1 cut(s) 3417
SapI GCTCTTC 1 cut(s) 1905
SaqAI TTAA 9 cut(s) 173, 803, 1400, 1811, 1991, 2331, 3332, 3413, 3816
Sau3AI GATC 9 cut(s) 202, 214, 370, 613, 940, 1312, 2125, 2398, 2627
ScaI AGTACT 1 cut(s) 2367
SchI GAGTC 2 cut(s) 1459, 2480
ScrFI CCNGG 6 cut(s) 54, 389, 424, 1772, 2436, 2874
SduI GDGCHC 5 cut(s) 39, 268, 2267, 2395, 3721
SfcI CTRYAG 2 cut(s) 438, 2235
SfuI TTCGAA 2 cut(s) 723, 3505
SinI GGWCC 3 cut(s) 385, 1084, 2590
SmiMI CAYNNNNRTG 4 cut(s) 1104, 1334, 2448, 3387
SmlI CTYRAG 2 cut(s) 172, 1142
SmoI CTYRAG 2 cut(s) 172, 1142
SnaBI TACGTA 1 cut(s) 1997
SpeI ACTAGT 1 cut(s) 3734
SspI AATATT 3 cut(s) 1168, 1731, 3787
SstI GAGCTC 1 cut(s) 268
StyD4I CCNGG 6 cut(s) 52, 387, 422, 1770, 2434, 2872
StyI CCWWGG 3 cut(s) 117, 664, 3283
TaqII GACCGA 1 cut(s) 2237
TatI WGTACW 3 cut(s) 1612, 2257, 2365
TauI GCSGC 3 cut(s) 50, 1908, 2213
TfiI GAWTC 3 cut(s) 976, 1517, 3620
Tru1I TTAA 9 cut(s) 173, 803, 1400, 1811, 1991, 2331, 3332, 3413, 3816
Tru9I TTAA 9 cut(s) 173, 803, 1400, 1811, 1991, 2331, 3332, 3413, 3816
TseFI GTSAC 8 cut(s) 40, 167, 1490, 2215, 2439, 2978, 3179, 3481
Tsp45I GTSAC 8 cut(s) 40, 167, 1490, 2215, 2439, 2978, 3179, 3481
TspGWI ACGGA 6 cut(s) 1563, 2274, 2661, 2806, 2852, 3080
Tth111I GACNNNGTC 2 cut(s) 1653, 3251
Van91I CCANNNNNTGG 3 cut(s) 1379, 2494, 3677
Vha464I CTTAAG 1 cut(s) 172
VpaK11BI GGWCC 3 cut(s) 385, 1084, 2590
XagI CCTNNNNNAGG 4 cut(s) 1775, 3242, 3287, 3649
XapI RAATTY 7 cut(s) 256, 818, 1396, 1505, 1597, 1766, 3798
XbaI TCTAGA 1 cut(s) 2395
XceI RCATGY 2 cut(s) 972, 2721
XcmI CCANNNNNNNNNTGG 1 cut(s) 3674
XmiI GTMKAC 3 cut(s) 1285, 2351, 3418
XmnI GAANNNNTTC 1 cut(s) 1680
ZrmI AGTACT 1 cut(s) 2367
Zsp2I ATGCAT 3 cut(s) 316, 1331, 3069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.