MD05G1107800.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005708
Physical Location & Seq
Reverse (-)
22108987 .. 22151563
42577 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1107800.v1.1.491

Sequence Viewer

Length: 7398 bp
ATGGCGGTCTCGGGGCAGTCCCTCAGGTTCTGCGGCCCCGCCTCTGCCGGACCCAGCGGAGGCAGTTTTGACGCGCTCAATCACATCCTTGCTGACCTCTGCACCCGCGGCAGCCCCAAGGATGGGGCTTCGCTAGCTTTAAAGAAGCATCTGGAAGAAGAAGCCCGTGACCTCAGCGGAGAAGCGTTTTCGCGTTACATGGATCAGTTGTATGATCGAATTTCGTCTCTTCTGGAGAGCAGTGATGTTGCAGAAACTTTGGGAGCTTTAAGAGCTATTGATGAGTTGATAGATGTGGTGCTGGGAGAGAATTCTTCCAAAGTTTCAAAGTTTGCTAATTATATAAGGACCGTTTTTGAGGTGAAGCATGATACTGATATTCTGGTCCTTGCTAGTAGAGTTCTGGGCCATCTTGCCAGGGCCGGGGGAGCAATGACTGCAGATGAAGTGGAACGCCAGATAAAAATTGCTCTTGATTGGTTGCGGGGGGATAGAGTTGAGTATCACCGTTTTGCTGCTGTGTTGATCTTGAAAGAAATGGCAGAAAATGCTTCAACAGTGTTCAATGTTCATGTACCTGAATTTGTTGATGCTATTTGGGTTGCTTTGAGGGATCCAGTCTTGCCTATTCGAGAGCGAGCTGTGGAAGCTCTACGTGCCTGCCTTGGTGTTATTGAGAAACGTGAGACACGCTGGCGTGTGCAATGGTATTATCGTATGTTTGAGGCTACCCAGGATGGGTTGGGTAGAAATGCTTCCGTTCACAGCATACATGGTTCTTTACTTGCAGTTGGAGAGCTTTTAAGGAATACAGGTGAATTTATGATGTCAAGGTACAGGGAAGTTGCAGAAATTGTCCTTAGATATCTTGAGCACCGGGATAGGCTGGTTCGCCTGAGCATAACTTCGCTGCTACCTCGAATTGCTCATTTTCTGCGAGATCGATTTGTCACAAACTACTTAACGATATGTATGAACCATATTCTTGCGGTTTTGCGAATACCGGCTGAACGCTCCAGTGGTTTTATTGCCCTTGGGGAGATGGCTGGTGCTTTGGATGGGGAACTTGTCCACTATTTGCCGACAATAACATCCCATTTACGTGATGCGATTGCTCCACGTAAAGGTAGACCCTCTCTTGAGGCTTTGGCTTGTGTTGGAAACATAGCAAAAGCAATGGGGCCACCTATGGAACCTCATGTTCGTGGTCTTTTGGATGTTATGTTGTCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCACTTGAGCATATAACTACCAGTATTCCATCTTTGCTTCCTACTATCCAAGATCGGTTGCTTGACAGCATTTCGGTGGTTCTTTCAAAATCCCACCATTCTCAGGGAAGGTCGGCCGTTGGCATGGGCAGAGGAAATTTAATGAATATCCCTCAGCAAGTCTCAGACGTCAGTGGTTCATCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTTAATTTCAAGGGCCATGACCTTCTTGAGTTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGATGGAGCCATCCGGAAAGATGCTGCCCTATGTTGTTGCAAATTAGTTGCAAATTCCTTTTCTGGTGTGCAATATGCTTCTGATAGGTCTAATCGGGGAAAACGACGACGTCTTGTTGAGGAGATTGTGGAAAAGCTTCTCATTGAAGCTGTTGCAGATGCTGATGTTATTGTTCGCCATTCAATATTTTCCTCCCTTCATGGGAATAGAGGATTTGATGATTTTTTGGTGCAGGCTGATAGTCTGAGCGCAGTTTTTGCTGCTTTGAATGATGAGGATTTTGATGTTCGAGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCGTATGTTCTTCCAGCACTTCGTCGGCATCTCATACAATTGTTGACTTACCTGGGGCAGAGTGCAGACACCAAATGCCGAGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGAAACTGTGAACGACTAATACTTCCATACGTTGCTCCGATACACAAGGCACTTGTTGCAAGGCTTACTGATGGCACTGGTGTGAACGCAAATAATGGCATTATTAGTGGAGTTCTTGTAACTGTTGGGGATCTGGCTAGAGTGGGTGGTTATGCAATGCGAATATACATTCCTGAGCTTATGCCTTTAATTGTTGAGGCGTTATTGGATGGAACAGCCGTCACCAAACGGGAAGTGGCTGTCGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACGCCATACAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTACTGAATGGTGAATTAGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTCGGAATTATGGGTGCTCTAGACCCTCATGTGCATAAACGACATCAGCAAAGCCTGCCCGGTTCACATGGAGATGTTACATGTAGTGCCAGTGAATCTGGTCAACACATTCAATCTGTGGATGAATTGCCTATGGACGTGTGGCCATCATTTGCAACGTCTGAAGACTATTATTCTACGGTTGCAATCAACTCCCTCATGCGTATACTTAGGGACCCTTCACTTGCTACTTACCACCTAAAGGTGGTCAGATCTCTTATGTTCATATTCAAGTCAATGGGTCTTGGTTGTGTTCCATACTTACCAAAGGTTTTACCTGATTTCTTCCATATAGTCCGGACATGTGATGATGCTTTAAAGGATTTTATAACATGGAAGCTTGGTACGCTAGTGTCTATTGTGCGTCAGCATATTGGGAAATATCTGCATGAGTTGCTCGTTCTAATTTCAGAATTATGGTCAACCTTCAGTTTTCCTGCTGCTGGCCGTCCTCAACTTGGTTATCCAGTTCTGCATCTGGTGGAGCAGCTTTGCTTGGCTCTCAATGATGAATTTCGAACGTACCTTCCGGATATTCTTCCATGTTGTATTCAAGTTCTAAGTGATGCAGAACGGTATAACGATTATACTTATGTTCTTGACATCCTCCATACCCTTGAAGTGTTTGGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCTGCACTCATTCGCTTGTTTAAAGTTGATGCCTCAGTGGACATAAGACATGCTGCTATTAAAACTTTGACGAAACTGATCCCTCATGTGCAGGTTACTGGTCACATATCTTCTCTCGTGCATCACTTAAAGCTAGTCTTAGATGGCAAGAATGATGAGCTCCGGAAGGATGCTGTTGATGCCCTTTATTGTCTAGCTCATGCCCTTGGGGAGGACTTCACAATTTTTATCCCATCAATACACAAACTTCTTCTGAGACATCGCCATAAGGAATTCGAGGAAATTGAAGGACGGTTGAAGCGACGTGAACCTCTAATTTTGGGGAGTACAACTATTCAAAGATTAAGCCGACGGCTTCCAGCAGAGGTTATTACTGACAATTGGAATGATTTAGAGACTGACCCTTATGATGTTGGGTCAGAAGTGCAGAAACAACTTCGAGGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAACGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACATTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCTTTACGAACCTGTGCAAGGCTTGCACAGCTGCAGCCATTTGTTGGGCGGGAGCTATTTGCAGCTGGTTTTGTTAGCTGTTGGGCACAGCTAAATGAGACTAGTCAGAAACAGTTAGTTCGAAGTTTGGAGATGGCATTTTCATCCCCAAATTTACCTCCTGAAATTTTGGCAACACTTCTTAACTTGGCAGAGTTCATGGAACACAATGAGAAGCCTCTTCCTATAGACATTCGCCTGCTTGGTGCTCTTGCAGAAAAGTGTTGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCGCGCTCCAAGAAAATGGACCCTGTTGCAGTAGTTGAAGATCTTATACATATAAACAACCAGTTACACCAGCATGAGGCTGCAGTTGGAATATTGACCTTTGCCCAACAAGATTTAGATGTTCAACTCAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGACGATGCTCTCAAGGCCTACACCGAAAAAGCCTCTCAAGCATCGAGTCCACATCTTGTTTTGGATGCCGCTTTAGGTCGGATGCGATGTCTTGCTGCTTTGGCACAATGGGAGGAGCTTAACAACCTGTTCAAGGAATATTGGACCCCAGCTGAACCAGCTGCCCGGTTGGAAATGGCACCAATGGCTGCTAATGCTGCTTGGAACATGGGGGAGTGGGATCAAATGGCAGAATACGTGTCGCGGTTAGATGATGGTGATGAAACCAAACTTAGGGGCTTGGGGAACACTGCTGCTAGTGGGGATGGAAGCAGTAATGGAACATTCTTTAGAGCTGTTCTGTTAGTTCAAAGAGGAAAGTATGATGAAGCACGTCAGTATGTTGAGAGAGCTAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATGGCAATATGGTCCGTGTTCAGCAGCTGTCAGAACTTGAGGAGGTCATTGATTATTGCACACTTCCTCTGGGAAACCCTGTTGCTGAAGGACGACGAGCACTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGCGCTTTTAGCAGTTAGAGCACTTGTTCTACCTCCAACAGAAGATATTGACACTTGGCTTAAGTATGCTTACCTTTGTCGGAAAAGTGGGCGAATTAGTCAGGCTAGATCTACTTTAGTCGAACTCTTACAGTATGATCCTGAAACATCTCCTGAAAGCGTGCGGTATGATGGGCCCCCACAAGTGATGCTTGCATATTTGGAATACCAATGGTCACTTGGAGAGGACATGAAGCGCAAGGAAGCATTTGCTAGGCTACAGAATTTGGCAATAGAGCTTTCAAGTCGACCTAGTATTCAACCGGTTACACCGTCTGTCTTGGTGAGCTCTAGTAGTCTGAGTGTTCCACTTATCGCCCGTGTGCATCTCATACTAGGAGACTGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCTCAATGCCTTCCGGACTGCAACTCAATGTGCCAATAAATGGGCAAAGGCATGGCACAAGTGGGCACTGTTTAATACAGCAGTGATGTCACTTTATACTGTAAGAGGTTATGCAAGTGTCGCGTCACAATTTGTTGTTGCAGCGGTCAATGGATATTTTCATTCGATAGCATGTTCAGCGAATACTAAAGGTGTTGATGATAGTTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTACTGCAGAAGTGCAAATGGCACTACAGAAAGGGTTTGCACATGTTAATATTAATACATGGCTGGTGGTTTTGCCTCAAATAATCGCCAGGATACATTCTAATAACAATGCTGTGAGGGAACTGATACATTCGCTTTTGGTTCGGATTGGACAAAGTCATCCACAGGCTCTTATGTACCCTCTCCTTGTAGCATGTAAATCAATAAGTAATTTACGTAAAGCTGCAGCTCAAGAAGTGGTTGACAAAGTTCGGCAGCATAGTGGTGTGCTTGTGGATCAGGCACAGCTTGTGTCAAAGGAACTAATCCGGGTCGCAATACTTTGGCATGAAAAGTGGCATGAGGCTCTGGAAGAAGCTAGTCGCCTGTATTTTGGTGAGCATAACATTGAGGGAATGCTAAAAGAATTGGAGCCACTCCATGAAATGCTTGAGGAAGGAGCTAAAGAGAACAATACAACCATAAAAGAGAGAGCATTCATTGAGGCATATCGTCATGAATTACTGGAGGCCTATGAGTGTTGCATGAAATTTAAGAGAACAGGGAAAGATGCTGAGCTTACTCAGGCTTGGGATCTTTACTATCATGTATTTAGACGAATAGATAAGCAGCTGCAGAGTCTTACTACTCTGGACTTAGAGTCCGTCTCACCAGAGTTGTTGGAATGTCGTAATTTGGAGCTCGCTGTTCCTGGGAGATATCGTGCTGAATCTCCTGTTGTAACAATTGCATCGTTTGCACGCCAACTTGTTGTCATAACATCCAAACAAAGACCAAGGAAATTGACGATTCGTGGGAGTGATGGTAAGGATTATGCCTTCTTGCTGAAGGGACATGAAGATTTACGCCAAGATGAACGCGTCATGCAGCTTTTTGGTTTGGTGAATACACTACTGGAGAATTCAACAAAAACTGAGGAGAAGGATCTCTCCATTGAACGATATGATGTCATTCCATTGTCTTCAAACAGTGGACTAATTGGTTGGGTTCCAAATTGTGACACCCTTAACCAGCTCATCAGGGAGTACAGAGATGCCAGAAAGATCACTCTAAATCAAGAGCACAAGTATATGCTAAGTTTTGCTCCGGATTATGATCGTTTGCCACTCATAGCTAAAGTGGAAGTCTTTGAGTATGCCCTGCACAATACAGAGGGAAACGACCTTGCAAGGGTCCTTTGGCTGAAAAGTCGCACTTCTGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTAGCAGTCATGAGCATGGTTGGGTACCTTCTTGGCTTAGGTGATCGGCACCCAAGCAATCTTATGCTGAACCGCTACAGTGGGAAGATCTTGCACATTGATTTTGGGGACTGCTTTGAGGCCTCTATGAACCGGGAAAAGTTCCCTGAGAAGGTCCCTTTTCGGTTGACTAGAATGCTTGTGAAAGCAATGGAGGTTAGTGGTATAGAGGGCAACTTCCGTTCAACATGTCAAAATGTGATGGAAGTCCTACGAATACATAAAGATAGCGTTATGGCTATGATGGAGGCCTTTGTCCATGATCCTCTCATTAATTGGCGTCTCTTCAACTTCAATGAAGTTCCCCAAATGTCAATGCTTGGAAATAGTAATGTTACTCCTGTTGCGGGTGCTGAAGAACCCGCTCAAAATAGAGAGCTTCGCCAACCTCAACGAGGTGCTCGTGAAAGGGAACTTCTTGAGGCTGTCAATCAATTGGGTGATGCTAATGAGGTCTTGAATGAGCTTGCTGTGGTTGTCATGGCTCGGATGAAGAGTAAACTTACTGGACGTGATTTTTCTGCCAGTTCCTCTGTATCTTCCAGCTCTATTCAACATGCAATTGACCACAGCACGCTGATTTCTGGAGACAGTAGAGAAGTTGACCATGGTTTATCTGTTAAGCAGCAAGTTCAAAAGTTGATTATCCAAGCATCATCTAGTGAAAATTTATGCCAAAATTATGTCGGGTGGTGTCCGTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

2466

Amino Acids

276.88

Weight (kDa)

6.19

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 758 - 926 2.4e-52 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1432 - 1798 1.9e-92 FAT domain
HEAT_ATR PF23593 1813 - 1892 3.9e-11 Serine/threonine-protein kinase ATR-like, HEAT repeats
FRB_dom PF08771 1905 - 2007 1.6e-36 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 2076 - 2324 9e-79 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2435 - 2465 1.7e-14 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2741
AatII GACGTC 2 cut(s) 1428, 1654
Acc36I ACCTGC 1 cut(s) 3157
Acc65I GGTACC 1 cut(s) 6675
AccB1I GGYRCC 3 cut(s) 4294, 6675, 6699
AccB7I CCANNNNNTGG 2 cut(s) 3686, 5202
AccBSI CCGCTC 2 cut(s) 4693, 7055
AccI GTMKAC 4 cut(s) 1129, 2348, 2578, 5017
AccII CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
AccIII TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
AcoI YGGCCR 3 cut(s) 1371, 2516, 2857
AcuI CTGAAG 6 cut(s) 2556, 2824, 3644, 4674, 6260, 7065
AcyI GRCGYC 3 cut(s) 1425, 1651, 6970
AflII CTTAAG 1 cut(s) 4790
AflIII ACRYGT 7 cut(s) 2453, 2511, 2714, 4353, 5455, 6271, 6878
AgeI ACCGGT 1 cut(s) 5032
AhdI GACNNNNNGTC 1 cut(s) 6052
AhlI ACTAGT 1 cut(s) 3743
AjiI CACGTC 5 cut(s) 2357, 2512, 3380, 4490, 7202
AjnI CCWGG 6 cut(s) 416, 732, 1929, 5131, 5501, 6103
AleI CACNNNNGTG 1 cut(s) 2072
AloI GAACNNNNNNTCC 2 cut(s) 2923, 2955
AlwNI CAGNNNCTG 3 cut(s) 30, 1703, 4594
Ama87I CYCGRG 1 cut(s) 10
Aor13HI TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
ApaI GGGCCC 1 cut(s) 4908
AseI ATTAAT 2 cut(s) 5466, 6963
AsiGI ACCGGT 1 cut(s) 5032
Asp700I GAANNNNTTC 2 cut(s) 754, 1677
Asp718I GGTACC 1 cut(s) 6675
AspLEI GCGC 6 cut(s) 76, 1793, 3958, 3960, 4735, 4968
AsuC2I CCSGG 6 cut(s) 424, 878, 2433, 4282, 5723, 6785
AsuII TTCGAA 2 cut(s) 2929, 3763
AsuNHI GCTAGC 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 9 cut(s) 50, 348, 385, 2587, 3973, 4260, 4579, 6586, 6805
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 3 cut(s) 3730, 4908, 5230
BaeI ACNNNNGTAYC 2 cut(s) 2748, 2781
BalI TGGCCA 1 cut(s) 2518
BamHI GGATCC 1 cut(s) 613
BanI GGYRCC 3 cut(s) 4294, 6675, 6699
BanII GRGCYC 5 cut(s) 3237, 4908, 5060, 5125, 6096
BarI GAAGNNNNNNTAC 4 cut(s) 739, 771, 3984, 4016
BauI CACGAG 2 cut(s) 3191, 7092
BbsI GAAGAC 3 cut(s) 2544, 5378, 6366
BbvCI CCTCAGC 2 cut(s) 173, 1410
BceAI ACGGC 4 cut(s) 1358, 2195, 2844, 3443
BciT130I CCWGG 6 cut(s) 418, 734, 1931, 5133, 5503, 6105
BciVI GTATCC 2 cut(s) 4689, 5499
BcnI CCSGG 6 cut(s) 424, 878, 2433, 4282, 5723, 6785
BcuI ACTAGT 1 cut(s) 3743
BfoI RGCGCY 1 cut(s) 4736
BfrI CTTAAG 1 cut(s) 4790
BfuAI ACCTGC 1 cut(s) 3157
BfuI GTATCC 2 cut(s) 4689, 5499
BglII AGATCT 4 cut(s) 2624, 3994, 4838, 6738
BlpI GCTNAGC 1 cut(s) 5967
BmcAI AGTACT 1 cut(s) 2364
Bme18I GGWCC 9 cut(s) 50, 348, 385, 2587, 3973, 4260, 4579, 6586, 6805
BmeRI GACNNNNNGTC 1 cut(s) 6052
BmeT110I CYCGRG 1 cut(s) 10
BmgBI CACGTC 5 cut(s) 2357, 2512, 3380, 4490, 7202
BmrI ACTGGG 2 cut(s) 1988, 2274
BmtI GCTAGC 1 cut(s) 137
BmuI ACTGGG 2 cut(s) 1988, 2274
BpiI GAAGAC 3 cut(s) 2544, 5378, 6366
BplI GAGNNNNNCTC 2 cut(s) 6044, 6076
BpmI CTGGAG 6 cut(s) 254, 1000, 5939, 6329, 6644, 7296
Bpu10I CCTNAGC 5 cut(s) 173, 896, 1410, 2166, 6688
Bpu1102I GCTNAGC 1 cut(s) 5967
Bpu14I TTCGAA 2 cut(s) 2929, 3763
BpuMI CCSGG 6 cut(s) 424, 878, 2433, 4282, 5723, 6785
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 5 cut(s) 656, 1103, 1121, 4354, 5630
BsaBI GATNNNNATC 1 cut(s) 6507
BsaHI GRCGYC 3 cut(s) 1425, 1651, 6970
BsaI GGTCTC 1 cut(s) 13
BsaWI WCCGGW 7 cut(s) 1554, 2709, 2941, 3237, 5032, 5175, 6499
BsaXI ACNNNNNCTCC 6 cut(s) 2094, 2124, 6100, 6130, 6202, 6232
Bse118I RCCGGY 2 cut(s) 1003, 5032
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 5 cut(s) 438, 710, 1182, 2154, 6846
Bse8I GATNNNNATC 1 cut(s) 6507
BseAI TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
BseBI CCWGG 6 cut(s) 418, 734, 1931, 5133, 5503, 6105
BseCI ATCGAT 1 cut(s) 943
BseJI GATNNNNATC 1 cut(s) 6507
BseMI GCAATG 5 cut(s) 438, 710, 1182, 2154, 6846
BsePI GCGCGC 1 cut(s) 3956
BseRI GAGGAG 5 cut(s) 1676, 4244, 4622, 6344, 6643
BseSI GKGCMC 3 cut(s) 3730, 4908, 5230
BseX3I CGGCCG 1 cut(s) 1371
BseYI CCCAGC 5 cut(s) 53, 301, 1853, 3551, 4264
BsgI GTGCAG 8 cut(s) 85, 1466, 1793, 1962, 3063, 3185, 3521, 6539
Bsh1236I CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
Bsh1285I CGRYCG 1 cut(s) 1374
BshNI GGYRCC 3 cut(s) 4294, 6675, 6699
BshTI ACCGGT 1 cut(s) 5032
BshVI ATCGAT 1 cut(s) 943
BsiEI CGRYCG 1 cut(s) 1374
BsiHKCI CYCGRG 1 cut(s) 10
BslFI GGGAC 6 cut(s) 4, 2600, 4130, 6258, 6773, 6791
BsmBI CGTCTC 3 cut(s) 231, 6064, 6977
BsmFI GGGAC 6 cut(s) 4, 2600, 4130, 6258, 6773, 6791
BsmI GAATGC 3 cut(s) 5814, 5888, 6831
Bso31I GGTCTC 1 cut(s) 13
BsoBI CYCGRG 1 cut(s) 10
Bsp119I TTCGAA 2 cut(s) 2929, 3763
Bsp120I GGGCCC 1 cut(s) 4904
Bsp13I TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
Bsp1407I TGTACA 1 cut(s) 2254
Bsp1720I GCTNAGC 1 cut(s) 5967
Bsp19I CCATGG 2 cut(s) 5407, 7297
BspDI ATCGAT 1 cut(s) 943
BspEI TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
BspFNI CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
BspHI TCATGA 2 cut(s) 5908, 6660
BspMAI CTGCAG 6 cut(s) 442, 3678, 4039, 5422, 5641, 6030
BspMI ACCTGC 1 cut(s) 3157
BspOI GCTAGC 1 cut(s) 137
BspT104I TTCGAA 2 cut(s) 2929, 3763
BspT107I GGYRCC 3 cut(s) 4294, 6675, 6699
BspTI CTTAAG 1 cut(s) 4790
BspTNI GGTCTC 1 cut(s) 13
BsrBI CCGCTC 2 cut(s) 4693, 7055
BsrDI GCAATG 5 cut(s) 438, 710, 1182, 2154, 6846
BsrFI RCCGGY 2 cut(s) 1003, 5032
BsrGI TGTACA 1 cut(s) 2254
BssAI RCCGGY 2 cut(s) 1003, 5032
BssHII GCGCGC 1 cut(s) 3956
BssNAI GTATAC 1 cut(s) 2579
BssNI GRCGYC 3 cut(s) 1425, 1651, 6970
BssSI CACGAG 2 cut(s) 3191, 7092
BssT1I CCWWGG 7 cut(s) 117, 664, 1033, 3280, 5407, 6188, 7297
Bst1107I GTATAC 1 cut(s) 2579
Bst2BI CACGAG 2 cut(s) 3191, 7092
Bst2UI CCWGG 6 cut(s) 418, 734, 1931, 5133, 5503, 6105
Bst6I CTCTTC 4 cut(s) 234, 3867, 6980, 7178
BstACI GRCGYC 3 cut(s) 1425, 1651, 6970
BstAFI CTTAAG 1 cut(s) 4790
BstAPI GCANNNNNTGC 8 cut(s) 437, 548, 1799, 2048, 2428, 2806, 3665, 6149
BstAUI TGTACA 1 cut(s) 2254
BstBAI YACGTR 5 cut(s) 656, 1103, 1121, 4354, 5630
BstBI TTCGAA 2 cut(s) 2929, 3763
BstDSI CCRYGG 3 cut(s) 106, 5407, 7297
BstENI CCTNNNNNAGG 4 cut(s) 3284, 3658, 4247, 7083
BstFNI CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
BstH2I RGCGCY 1 cut(s) 4736
BstHHI GCGC 6 cut(s) 76, 1793, 3958, 3960, 4735, 4968
BstMCI CGRYCG 1 cut(s) 1374
BstNI CCWGG 6 cut(s) 418, 734, 1931, 5133, 5503, 6105
BstNSI RCATGY 8 cut(s) 2457, 2718, 3128, 5337, 5459, 5610, 6882, 7250
BstSLI GKGCMC 3 cut(s) 3730, 4908, 5230
BstSNI TACGTA 1 cut(s) 5630
BstUI CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
BstV2I GAAGAC 3 cut(s) 2544, 5378, 6366
BstX2I RGATCY 8 cut(s) 613, 2122, 2624, 3994, 4838, 5986, 6337, 6738
BstXI CCANNNNNNTGG 1 cut(s) 3970
BstYI RGATCY 8 cut(s) 613, 2122, 2624, 3994, 4838, 5986, 6337, 6738
BstZ17I GTATAC 1 cut(s) 2579
BstZI CGGCCG 1 cut(s) 1371
Bsu15I ATCGAT 1 cut(s) 943
Bsu36I CCTNAGG 1 cut(s) 23
BsuI GTATCC 2 cut(s) 4689, 5499
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 3 cut(s) 106, 5407, 7297
BtgZI GCGATG 3 cut(s) 3320, 4126, 4216
BtrI CACGTC 5 cut(s) 2357, 2512, 3380, 4490, 7202
BtsI GCAGTG 4 cut(s) 247, 3546, 4404, 5250
BveI ACCTGC 1 cut(s) 3157
CaiI CAGNNNCTG 3 cut(s) 30, 1703, 4594
CciI TCATGA 2 cut(s) 5908, 6660
CfoI GCGC 6 cut(s) 76, 1793, 3958, 3960, 4735, 4968
Cfr10I RCCGGY 2 cut(s) 1003, 5032
Cfr42I CCGCGG 1 cut(s) 109
ClaI ATCGAT 1 cut(s) 943
CseI GACGC 5 cut(s) 80, 2765, 5274, 6262, 6959
CspAI ACCGGT 1 cut(s) 5032
CspCI CAANNNNNGTGG 2 cut(s) 1830, 1865
DraI TTTAAA 3 cut(s) 141, 2730, 3097
DriI GACNNNNNGTC 1 cut(s) 6052
EaeI YGGCCR 3 cut(s) 1371, 2516, 2857
EagI CGGCCG 1 cut(s) 1371
Eam1104I CTCTTC 4 cut(s) 234, 3867, 6980, 7178
Eam1105I GACNNNNNGTC 1 cut(s) 6052
EarI CTCTTC 4 cut(s) 234, 3867, 6980, 7178
Ecl136II GAGCTC 3 cut(s) 3235, 5058, 6094
EclXI CGGCCG 1 cut(s) 1371
Eco105I TACGTA 1 cut(s) 5630
Eco130I CCWWGG 7 cut(s) 117, 664, 1033, 3280, 5407, 6188, 7297
Eco147I AGGCCT 5 cut(s) 3923, 4133, 5924, 6773, 6941
Eco24I GRGCYC 5 cut(s) 3237, 4908, 5060, 5125, 6096
Eco31I GGTCTC 1 cut(s) 13
Eco32I GATATC 2 cut(s) 866, 6113
Eco47I GGWCC 9 cut(s) 50, 348, 385, 2587, 3973, 4260, 4579, 6586, 6805
Eco52I CGGCCG 1 cut(s) 1371
Eco53kI GAGCTC 3 cut(s) 3235, 5058, 6094
Eco57I CTGAAG 6 cut(s) 2556, 2824, 3644, 4674, 6260, 7065
Eco81I CCTNAGG 1 cut(s) 23
Eco88I CYCGRG 1 cut(s) 10
EcoICRI GAGCTC 3 cut(s) 3235, 5058, 6094
EcoNI CCTNNNNNAGG 4 cut(s) 3284, 3658, 4247, 7083
EcoO109I RGGNCCY 4 cut(s) 2587, 4905, 6586, 6805
EcoRI GAATTC 3 cut(s) 310, 3347, 6313
EcoRII CCWGG 6 cut(s) 416, 732, 1929, 5131, 5501, 6103
EcoRV GATATC 2 cut(s) 866, 6113
EcoT14I CCWWGG 7 cut(s) 117, 664, 1033, 3280, 5407, 6188, 7297
EcoT22I ATGCAT 1 cut(s) 3066
EcoT38I GRGCYC 5 cut(s) 3237, 4908, 5060, 5125, 6096
ErhI CCWWGG 7 cut(s) 117, 664, 1033, 3280, 5407, 6188, 7297
Esp3I CGTCTC 3 cut(s) 231, 6064, 6977
FalI AAGNNNNNCTT 6 cut(s) 3197, 3229, 4905, 4937, 6592, 6624
FaqI GGGAC 6 cut(s) 4, 2600, 4130, 6258, 6773, 6791
FauI CCCGC 6 cut(s) 46, 113, 477, 3684, 7030, 7060
FauNDI CATATG 2 cut(s) 3060, 4567
FblI GTMKAC 4 cut(s) 1129, 2348, 2578, 5017
FriOI GRGCYC 5 cut(s) 3237, 4908, 5060, 5125, 6096
GlaI GCGC 6 cut(s) 75, 1792, 3957, 3959, 4734, 4967
GsaI CCCAGC 5 cut(s) 57, 305, 1857, 3555, 4268
GsuI CTGGAG 6 cut(s) 254, 1000, 5939, 6329, 6644, 7296
HaeII RGCGCY 1 cut(s) 4736
HgaI GACGC 5 cut(s) 80, 2765, 5274, 6262, 6959
HhaI GCGC 6 cut(s) 76, 1793, 3958, 3960, 4735, 4968
Hin1I GRCGYC 3 cut(s) 1425, 1651, 6970
Hin6I GCGC 6 cut(s) 74, 1791, 3956, 3958, 4733, 4966
HinP1I GCGC 6 cut(s) 74, 1791, 3956, 3958, 4733, 4966
HincII GTYRAC 7 cut(s) 1923, 2477, 2835, 5018, 5656, 6819, 7294
HindII GTYRAC 7 cut(s) 1923, 2477, 2835, 5018, 5656, 6819, 7294
HindIII AAGCTT 2 cut(s) 1676, 2750
Hpy99I CGWCG 6 cut(s) 1650, 1653, 1905, 3381, 3429, 4665
Hsp92I GRCGYC 3 cut(s) 1425, 1651, 6970
HspAI GCGC 6 cut(s) 74, 1791, 3956, 3958, 4733, 4966
KflI GGGWCCC 1 cut(s) 2587
Kpn2I TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
KpnI GGTACC 1 cut(s) 6679
KspI CCGCGG 1 cut(s) 109
MbiI CCGCTC 2 cut(s) 4693, 7055
MfeI CAATTG 5 cut(s) 1916, 3454, 6138, 7124, 7251
MflI RGATCY 8 cut(s) 613, 2122, 2624, 3994, 4838, 5986, 6337, 6738
MlsI TGGCCA 1 cut(s) 2518
MluI ACGCGT 1 cut(s) 6271
MluNI TGGCCA 1 cut(s) 2518
MlyI GAGTC 5 cut(s) 1456, 4097, 4171, 6040, 6062
MmeI TCCRAC 8 cut(s) 772, 1138, 4021, 4175, 4266, 4790, 4790, 6054
Mox20I TGGCCA 1 cut(s) 2518
Mph1103I ATGCAT 1 cut(s) 3066
MroI TCCGGA 6 cut(s) 1554, 2709, 2941, 3237, 5175, 6499
MroXI GAANNNNTTC 2 cut(s) 754, 1677
MscI TGGCCA 1 cut(s) 2518
MslI CAYNNNNRTG 8 cut(s) 1101, 1203, 1331, 2072, 2445, 4026, 5676, 6665
Msp20I TGGCCA 1 cut(s) 2518
MspCI CTTAAG 1 cut(s) 4790
MunI CAATTG 5 cut(s) 1916, 3454, 6138, 7124, 7251
Mva1269I GAATGC 3 cut(s) 5814, 5888, 6831
MvaI CCWGG 6 cut(s) 418, 734, 1931, 5133, 5503, 6105
MvnI CGCG 7 cut(s) 74, 108, 193, 3958, 4360, 5285, 6273
NciI CCSGG 6 cut(s) 424, 878, 2433, 4282, 5723, 6785
NcoI CCATGG 2 cut(s) 5407, 7297
NdeI CATATG 2 cut(s) 3060, 4567
NheI GCTAGC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 1982
NmuCI GTSAC 8 cut(s) 167, 949, 2212, 3176, 4944, 5250, 5286, 6410
NsiI ATGCAT 1 cut(s) 3066
NspI RCATGY 8 cut(s) 2457, 2718, 3128, 5337, 5459, 5610, 6882, 7250
NspV TTCGAA 2 cut(s) 2929, 3763
OliI CACNNNNGTG 1 cut(s) 2072
PagI TCATGA 2 cut(s) 5908, 6660
PauI GCGCGC 1 cut(s) 3956
PceI AGGCCT 5 cut(s) 3923, 4133, 5924, 6773, 6941
PciI ACATGT 4 cut(s) 2453, 2714, 5455, 6878
PcsI WCGNNNNNNNCGW 3 cut(s) 1642, 3373, 7090
PctI GAATGC 3 cut(s) 5814, 5888, 6831
PdmI GAANNNNTTC 2 cut(s) 754, 1677
PfeI GAWTC 6 cut(s) 1514, 2468, 3629, 5147, 6122, 6202
PflFI GACNNNGTC 2 cut(s) 1650, 5568
PflMI CCANNNNNTGG 2 cut(s) 3686, 5202
PfoI TCCNGGA 1 cut(s) 5131
PinAI ACCGGT 1 cut(s) 5032
PleI GAGTC 5 cut(s) 1456, 4096, 4170, 6039, 6061
PpsI GAGTC 5 cut(s) 1456, 4096, 4170, 6039, 6061
Ppu21I YACGTR 5 cut(s) 656, 1103, 1121, 4354, 5630
PpuMI RGGWCCY 3 cut(s) 2587, 6586, 6805
PscI ACATGT 4 cut(s) 2453, 2714, 5455, 6878
PshBI ATTAAT 2 cut(s) 5466, 6963
PsiI TTATAA 1 cut(s) 2741
Psp124BI GAGCTC 3 cut(s) 3237, 5060, 6096
Psp5II RGGWCCY 3 cut(s) 2587, 6586, 6805
Psp6I CCWGG 6 cut(s) 416, 732, 1929, 5131, 5501, 6103
PspFI CCCAGC 5 cut(s) 53, 301, 1853, 3551, 4264
PspGI CCWGG 6 cut(s) 416, 732, 1929, 5131, 5501, 6103
PspOMI GGGCCC 1 cut(s) 4904
PspPPI RGGWCCY 3 cut(s) 2587, 6586, 6805
PsrI GAACNNNNNNTAC 4 cut(s) 1995, 2027, 2355, 2387
PstI CTGCAG 6 cut(s) 442, 3678, 4039, 5422, 5641, 6030
PstNI CAGNNNCTG 3 cut(s) 30, 1703, 4594
PsuI RGATCY 8 cut(s) 613, 2122, 2624, 3994, 4838, 5986, 6337, 6738
PsyI GACNNNGTC 2 cut(s) 1650, 5568
PteI GCGCGC 1 cut(s) 3956
PvuII CAGCTG 6 cut(s) 3673, 3707, 4268, 4277, 4594, 6025
RseI CAYNNNNRTG 8 cut(s) 1101, 1203, 1331, 2072, 2445, 4026, 5676, 6665
SacI GAGCTC 3 cut(s) 3237, 5060, 6096
SacII CCGCGG 1 cut(s) 109
SalI GTCGAC 1 cut(s) 5016
ScaI AGTACT 1 cut(s) 2364
SchI GAGTC 5 cut(s) 1456, 4097, 4171, 6040, 6062
Sfr303I CCGCGG 1 cut(s) 109
SfuI TTCGAA 2 cut(s) 2929, 3763
SgrBI CCGCGG 1 cut(s) 109
SinI GGWCC 9 cut(s) 50, 348, 385, 2587, 3973, 4260, 4579, 6586, 6805
SmiMI CAYNNNNRTG 8 cut(s) 1101, 1203, 1331, 2072, 2445, 4026, 5676, 6665
SnaBI TACGTA 1 cut(s) 5630
SpeI ACTAGT 1 cut(s) 3743
SseBI AGGCCT 5 cut(s) 3923, 4133, 5924, 6773, 6941
SspI AATATT 4 cut(s) 1728, 4047, 4256, 5464
SstI GAGCTC 3 cut(s) 3237, 5060, 6096
StuI AGGCCT 5 cut(s) 3923, 4133, 5924, 6773, 6941
StyI CCWWGG 7 cut(s) 117, 664, 1033, 3280, 5407, 6188, 7297
TatI WGTACW 4 cut(s) 2254, 2362, 3401, 6438
TauI GCSGC 3 cut(s) 36, 111, 4187
TfiI GAWTC 6 cut(s) 1514, 2468, 3629, 5147, 6122, 6202
TseFI GTSAC 8 cut(s) 167, 949, 2212, 3176, 4944, 5250, 5286, 6410
Tsp45I GTSAC 8 cut(s) 167, 949, 2212, 3176, 4944, 5250, 5286, 6410
TspGWI ACGGA 5 cut(s) 748, 4571, 6046, 6860, 7377
Tth111I GACNNNGTC 2 cut(s) 1650, 5568
Van91I CCANNNNNTGG 2 cut(s) 3686, 5202
Vha464I CTTAAG 1 cut(s) 4790
VpaK11BI GGWCC 9 cut(s) 50, 348, 385, 2587, 3973, 4260, 4579, 6586, 6805
VspI ATTAAT 2 cut(s) 5466, 6963
XagI CCTNNNNNAGG 4 cut(s) 3284, 3658, 4247, 7083
XbaI TCTAGA 1 cut(s) 2392
XceI RCATGY 8 cut(s) 2457, 2718, 3128, 5337, 5459, 5610, 6882, 7250
XcmI CCANNNNNNNNNTGG 2 cut(s) 2224, 4946
XmiI GTMKAC 4 cut(s) 1129, 2348, 2578, 5017
XmnI GAANNNNTTC 2 cut(s) 754, 1677
ZraI GACGTC 2 cut(s) 1426, 1652
ZrmI AGTACT 1 cut(s) 2364
Zsp2I ATGCAT 1 cut(s) 3066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.