RchiOBHm_Chr6g0247951

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0005708
Physical Location & Seq
Forward (+)
3943618 .. 3969896
26279 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22228

Sequence Viewer

Length: 7395 bp
ATGGCCGTCGCGGTGCAGTCCCTCAGGTTCGGCGGGCCCGTCACCGCCGCACCCGGCAGCGGAAGCTTCGACGCCCTCAATCGTATCCTCGCCGACCTCTGCACGCGTGGCAACCCCAAGGAGGGGGCTTCGTTGGCTTTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACTTAAGTGGAGAAGCATTTTCTCGTTTCATGGATCAGTTGTATGATCGGATTTCTGCTCTTTTGGATAGCAGTGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGACGAGTTGATAGATGTGGCACTTGGAGAGAATGCATCAAAGGTTTCAAAGTTTGCTAATTACATAAGGACTGCCTTTGAGCTAAAACGTGATCCTGACATTTTGGTCCTGGCTAGTAGAGTTTTGGGCCATCTAGCTAGGGCCGGGGGAGCAATGACTGCAGATGAAGTGGAATGCCAGATAAAAATTGCTCTGGAATGGTTGCGTGGAGACAGAATTGAGTATCGACGTTTTGCTGCCGTCTTGATTTTGAAAGAGATGGCAGAAAATGCTTCCACAGTGTTCAATGTTCATGTACCTGAGTTTGTTGATGCTATCTGGGTTGCTTTGAGAGATCCAGTATTGGCTATCCGAGAGCGAGCTGTGGAAGCTCTGCGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGATGGCGTGTGCAATGGTATTATCGTATGTTCGAAGCTACACAGGATGGATTGGGTAAAAATGCTTCTGTTCACAGCATACATGGTTCTTTACTTGCAGTTGGAGAGCTTTTAAGGAATACAGGTGAATTCATGATGTCAAGGTATAGGGAAGTTGCAGAAATTGTCCTTAGGTATCTTGACCACAAGGATAGGCTGGTTCGCCTGAGCATAACTTCACTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTTACAAACTACTTAGAGACATGTATGAATCATATCCTCGCTGTTTTGCGACAATCAGCCGAACTACGCTCCAGTGGTTTTATTGCACTTGGGGAGATGGCTGGTGCTTTGGACGGGGAACTGTTTTTCTATTTGGGCCAAATTACACCTCACTTACGTGAAGCAATTGCTCCACGTAGAGGGCGACCCTCACTTGAGGCTTTGGCTTGTGTTGGAAATATTGCAAAAGCAATGGGGCCTGCTATGGAACATGATGTTCGTGGTCTTTTGGATGTTATGTTCGCTGCTGGTCTTTCCTCTACACTTGTTGAGGCCCTTGAAAAGATAACTGCCAGTATACCATCTTTGCTGCCTACCATCCAAGATCGGTTGCTAGAATGCATTTCAGTGGTTCTTTCAAAATCCCAACATCCTCAGGGAAGGCCAGTTGTTGGAATGGGTAGAGGAAATTTAATAAATATCCCTCAGCAAGCTTCAGACCTCAGTGGCTCATCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTCAATTTCAAGGGTCACGACCTTCTTGAATTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGACGGAGCCATCCGAAAAGATGCTGCCCTTTGTTGTTGCAGATTGGTTGCAAATTCCTTTTCTGGTGTACAATATGCTTCTGGTAGGTCAAATCGGGGAAAGCGACGCCGTCTTGTCGAGGAGATTGTTGAAAAGCTTCTCATTGAGGCTGTTGCAGATGCTGATGTGATTGTTCGCCATTCAATATTTTCTTCTCTACATGGGAATAGAGGCTTTGACGAATTCCTGGCTCAGGCTGATAGTCTCAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGCGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCTTATGTTCTTCCAGCTCTTCGCCGCCATCTTATACAGTTGTTGACTTACTTGGGTCAGAGTGCAGATAGCAAATGCAGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGTAACTGTGAAAGACTAATACTTCCATACATTGCTCCTATACACAAGGCACTTGTGGCAAGACTTATGGATGGCACTGGTGTCGGCTCAAATAATGGCATCATTAGTGGAGTTCTTGTAACTGTTGGGGATCTTGCTAGAGTGGGTGGGTTTGCAATGAGAAAATATATACCAGAGCTTATGCCTTTGATCGTTGATGCTTTATTGGATGGAGCGGCTGTCACAAAACGGGAAGTGGCTGTAGCTACTCTCGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCGTATAATGAATATCCACTGTTGCTTGGCTTACTCTTGAAGTTGCTTAACGGTGAGTTGGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTTGGAATTATGGGTGCTCTAGATCCTCATGTGCATAAACGAAATCAACAAAGCTTGCCTGGGTCACATGGAGATGTTCCTCGTAATGCCAGTGATTCTGGTCAACACATCCAATCTGTGGATGAATTGCCTATGGACTTGTGGCCATCATTTGCAACCTCTGAAGATTATTATTCTACGGTTGCAATCAATTCTCTCATGCGAATACTTAGGGACCCTTCACTTGGTACTTACCACCTAAAGGTTGTTGGATCACTTATGTTCATATTCAAGTCAATGGGTCTCGGTTGTGTGCCGTACTTACCAAAGGTGTTGCCTGACCTTTTCCATATAGTCCGCACATGTGATGATGCTTTGAAGGATTTTATCACATGGAAGCTTGGAACTCTAGTGTCTATTGTGCGGCAGCACATACGGAAATATCTGCATGAATTGTTGGCTCTAATTTCAGAACTATGGTCAACCTTTAGTTTCCCTGCTGGTGTCCGTCCTCAACCTGGCTATCCAGTTCTTCATCTGGTGGAGCAGCTTTGTTTGGCCCTGAATGATGAGTTCAGGACGTATCTTCCTGATATTCTTCCATGCTGTATCCAAGTTCTAAGTGACGCAGAACGGTGTAATGACTACACTTACGTTCTTGACATCCTCCATACCCTTGAAGTATTTAGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCCGCTCTAATCCGTTTGTTCAAAGTGGATGCTTCAGTGGATATAAGACGTGCTGCTATCAAAACTTTGACCAAACTCATCCCTCGGGTGCAGGTTACTGGTCACATATCTTCTCTTGTGCATCACTTGAAGCTAGTCTTAGATGGGAAGAATGATGACCTCCGGAAGGACACTGTCGATGCTCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGATTTCACCATCTTTATCCCATCCATACACAAACTTTTGTTAAAACATCGCTTACGGCACAAGGGGTTTGAGGAAATTGAAGGCCATTTGCAGCGACGTGAACCTTTTGCTCCCCAAAGATTAAGTCGACGACTTCCAGAGGTTGTTGCTGACCGTTCAACTGATTTGGAGATTGACCCTTATGATGATGTGACTGATGTGCAGAAAAAACTTCGAAGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACACTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCACTACGAACCTGTGCAAGGCTTGCACAGTTGCAGCCATTTGTTGGGCGGGAGTTGTTTGCAGCTGGATTTGTTAGCTGTTGGGCACAGCTAAACGAGACTAGTCAGAAACAGTTAGTTCGGAGTTTGGAGATGGCATTTTCATCTCCAAATATTCCTCCTGAAATTTTGGCAACACTGCTTAATCTGGCAGAGTTCATGGAACATGATGAGAAACCCCTTCCTATAGACATTCGCCTACTTGGTGCTCTTTCAGAGAAGTGTCGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCGCGTTCCAAGAAGATGGATGCCAATCCTGTTGCTGTAGTTGAAGCACTTATACATATAAACAACCAGTTACAACAGCATGAGGCTGCAGTTGGAATATTGACCTATGCCCAGCAAAATTTGGATGTTCAACTGAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGATGATGCTCTCAAGGCCTACACTGCAAAAGCATCTCAAGCATCAAGTCAACATCTTATTTTGGATGCTACTTTAGGTCGTATGCGATGCCTTGCTGCCTTGGCTCGATGGGAAGAGCTTAACAACCTGTTTAAGGAATACTGGACCCCAGCTGAGCCTGCTGCTCGACTGGAAATGGCCCCAATGGCTGCAAGTGCTGCTTGGAATATGGGAGAGTGGGATCAAATGGCCGAATATGTGTCTCGATTGGACGATGGCGACGAAACCAAACTTAGGGGATTGGGGAACACTGCTGCTAGCGGAGATGGAAGCAGTAATGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGAGGCAAGTATGATGAAGCACGTGAGTATGTTGAGAGAGCCAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATATCAATATGGTTCGTGTTCAGCAGCTGTCAGAACTTGAGGAGGTGATCGATTATTGTACACTTCCTCTGGGGAATCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGTGCTTTTAGCAGTGAGAGCACTTGTTCTACCTCCTACTGAAGATGTTGATACTTGGCTAAAGTTTGCTACCCTCTGTCGGAAGAGTGGGCGACTTAGCCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTGTGACCCCGAGACATCTCATGAAAGCGTGCGATACCATGGGCCTCCACAAGTGATGCTTGCATACTTGAAATACCAATGGTCACTTGGAGAAGACCTCAAACGCAAGGAAGCGTTCGCTAGGCTGCAGAATTTGGCAATGGAGCTCTCAACTTCACCTAGTATTGAATCAGTTACGCCAACTGGCTTGATGAGCTGTAGTACTCCAAGTGTTCCACTCATTGCCCGTGTGTATCTCAAACTCGGAGAATGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCGTGTTGCCTTCCGAAATGCAACTCAATGTGCAAATAAATGGGCAAAGGCTTGGCACACATGGGCATTGTTCAATACAGCTGTTATGTCTCTTTATACTGTCAGAGGGTATGCAAGTGCTGCTTCCCAGTTTGTTGTTGCAGCAGTCACTGGATATTTTCACTCAATCGCCTGTTCAGCAAATACTAAAGGTGTGGATGATAGCTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTAGTGCTGAAGTTCAAATGGCATTACAAAGAGGGTTTGCACATGTTAATATTAATACATGGCTGGTGGTTTTACCCCAAATAATTGCCAGGATACATTCTAATAATCATGCTGTAAGGGAACTCATACAGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGGCTCTCATGTACCCTCTCCTAGTAGCCTGTAAATCAATAAGTAATTTACGCAAAGCTGCCGCTCAAGAAGTGGTTGACAAAGTTCGACAGCATAGTGGTTTACTTGTGGATCAGGCACAACTTGTTTCAACAGAACTAATCCGGGTCGCAATACTTTGGCATGAAATGTGGCATGAGGCTCTGGAAGAAGCTAGTCGTCTGTATTTTGGTGAACATAACATTGAGGGCATGCTAAAAGTATTGGAGCCATTGCATGAAATGCTCGAGGAAGGGGCTATGAAGAATAATACCACTATAAAAGAGACAACATTCATTGAGGCCTATCGTCATGAATTGCTGGAGGCTTATGAATGTTGCATGAAATATAAGAGAACTGGAAAAGATGCCGAGCTTACTCAGGCATGGGATCTTTACTATCATGTATTCAGACGAATAGATAAGCAGCTGCAAAGTCTCACTACATTGGACTTGGAGTCTGTCTCACCAGAGTTGCTAGAATGTCGTGATTTGGAGCTGGCTGTTCCTGGTACATATCGAGCAGAATCTCCGGTGGTGACAATTAGATCATTTGCACGCCAGCTTGTTGTCATAACATCCAAACAGCGACCAAGGAAATTAACAATTCATGGGAGTGATGGTGAGGAATATGCCTTCTTGCTGAAGGGACATGAAGACTTGCGCCAAGATGAACGTGTCATGCAGCTTTTTGGTTTGGTGAACACGCTGCTGGAGAATTCTAGAAAAACTCAAGAGAAGGATCTCTCCATTCAACGATATTCTGTCATTCCTTTATCTCCAAACAGTGGACTGATTGGTTGGGTCCCAAATTGTGACACCCTTCACCATCTCATTAGGGAGTACAGGGATGCCAGAAAGATCACTCTTAATCAAGAACACAAATATATGCTCAGTTTTGCTCCGGATTATGATCATTTGCCACTCATAGCTAAAGTGGAAGTATTTGAGTATGCCCTGCACAATACAGAGGGAAATGACCTTTCAAGGGTTCTTTGGCTGAAAAGTCGCACCTCAGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTGGCAGTCATGAGCATGGTTGGTTACCTTCTTGGTCTCGGTGATCGTCACCCAAGCAATCTTATGCTGCACCGCTATAGTGGGAAGATCTTGCACATTGACTTTGGGGACTGCTTTGAAGCATCTATGAATCGCGAAAAGTTTCCTGAGAAGGTCCCCTTTCGATTGACTAGAATGCTTGTGAAAGCAATGGAGGTTAGTGGTATAGAGGGCAACTTCCGTTCAACTTGTGAAAATGTCATGCAAGTTCTGCGAACACATAAAGATAGTGTTATGGCTATGATGGAGGCCTTTGTTCATGATCCTCTCATCAACTGGCGTCTCTTCAACTTCAATGAAGTTCCACAAGTGGCAATGCTAGCAAATAGTCATGTTCCTCCTGTTGTGGATGCCGAAGAACCGGCTCCAGCTAGAGAGCTTCCCCAACCTCAACGTGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTTAATCAACTTGGTGATGCTAATGAGGTCTTAAATGAGCGTGCTGTGGTTGTCATGGCTCGGATGAGTAATAAGCTTACTGGGCGTGATTTTTCCACCAGCTCCTCTGTATCATCTAGCTCTATTCAACATGCAGTAGACCACAGCACGCTGATTTCTGGAGATACTCGAGAAGTTGATCATGGTTTATCTGTTAAGCTGCAAGTTCAAAAGTTGATTGGCCAAGCAACTTCTCATGAAAATTTGTGCCAGAATTATGTCGGGTGGTGTCCTTTTTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

2464

Amino Acids

276.77

Weight (kDa)

6.45

Isoelectric Point (pI)

42.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 759 - 927 1.2e-51 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1431 - 1797 1.7e-95 FAT domain
HEAT_ATR PF23593 1812 - 1891 6.2e-11 Serine/threonine-protein kinase ATR-like, HEAT repeats
FRB_dom PF08771 1904 - 2006 5.5e-38 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 2074 - 2323 2.4e-82 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2434 - 2464 1.8e-14 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 4717
AasI GACNNNNNNGTC 1 cut(s) 383
Acc36I ACCTGC 2 cut(s) 3160, 4717
AccB7I CCANNNNNTGG 4 cut(s) 1379, 2494, 3677, 6380
AccBSI CCGCTC 4 cut(s) 2210, 3083, 4690, 5639
AccI GTMKAC 4 cut(s) 1285, 2351, 3418, 7251
AccII CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
AccIII TCCGGA 2 cut(s) 3240, 6496
AcoI YGGCCR 4 cut(s) 3, 2519, 4341, 7333
AcuI CTGAAG 9 cut(s) 1407, 2559, 3096, 3635, 4671, 4788, 5439, 6257, 7091
AcvI CACGTG 1 cut(s) 4487
AcyI GRCGYC 3 cut(s) 72, 1651, 6967
AflII CTTAAG 1 cut(s) 172
AflIII ACRYGT 5 cut(s) 104, 968, 2717, 5452, 6268
AhdI GACNNNNNGTC 1 cut(s) 6049
AhlI ACTAGT 1 cut(s) 3734
AjiI CACGTC 3 cut(s) 2360, 3128, 3389
AjnI CCWGG 9 cut(s) 387, 1770, 2434, 2872, 4505, 4827, 5128, 5498, 6100
AleI CACNNNNGTG 1 cut(s) 1104
AloI GAACNNNNNNTCC 2 cut(s) 1211, 1243
Alw21I GWGCWC 7 cut(s) 268, 2267, 2395, 3883, 4750, 5007, 7090
AlwNI CAGNNNCTG 3 cut(s) 1706, 4591, 5309
Ama87I CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
Aor13HI TCCGGA 2 cut(s) 3240, 6496
ApaI GGGCCC 2 cut(s) 39, 4666
ArsI GACNNNNNNTTYG 2 cut(s) 3400, 3432
AseI ATTAAT 1 cut(s) 5463
Asp700I GAANNNNTTC 3 cut(s) 1680, 6789, 7104
AspLEI GCGC 4 cut(s) 1796, 3561, 3949, 6258
AsuC2I CCSGG 3 cut(s) 54, 424, 5720
AsuII TTCGAA 3 cut(s) 723, 3505, 4462
AsuNHI GCTAGC 2 cut(s) 4409, 7006
AvaI CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
AvaII GGWCC 5 cut(s) 385, 2590, 4257, 6397, 6802
AxyI CCTNAGG 3 cut(s) 23, 860, 1362
BaeGI GKGCMC 3 cut(s) 39, 3721, 4666
BalI TGGCCA 2 cut(s) 2521, 7335
BanII GRGCYC 5 cut(s) 39, 268, 4666, 5007, 5122
BarI GAAGNNNNNNTAC 4 cut(s) 739, 771, 3981, 4013
BauI CACGAG 1 cut(s) 7089
BbrPI CACGTG 1 cut(s) 4487
BbsI GAAGAC 3 cut(s) 4959, 5375, 6255
Bbv12I GWGCWC 7 cut(s) 268, 2267, 2395, 3883, 4750, 5007, 7090
BbvCI CCTCAGC 1 cut(s) 1413
BceAI ACGGC 4 cut(s) 503, 1638, 2656, 3362
BcgI CGANNNNNNTGC 2 cut(s) 2059, 2093
BciT130I CCWGG 9 cut(s) 389, 1772, 2436, 2874, 4507, 4829, 5130, 5500, 6102
BciVI GTATCC 4 cut(s) 95, 2975, 4686, 5496
BclI TGATCA 2 cut(s) 6505, 7291
BcnI CCSGG 3 cut(s) 54, 424, 5720
BcuI ACTAGT 1 cut(s) 3734
BfmI CTRYAG 8 cut(s) 438, 2235, 3858, 3981, 4032, 4985, 5056, 6724
BfrI CTTAAG 1 cut(s) 172
BfuAI ACCTGC 2 cut(s) 3160, 4717
BfuI GTATCC 4 cut(s) 95, 2975, 4686, 5496
BglI GCCNNNNNGGC 1 cut(s) 4298
BglII AGATCT 2 cut(s) 4835, 6735
BlpI GCTNAGC 1 cut(s) 4266
BmcAI AGTACT 2 cut(s) 2367, 5062
Bme18I GGWCC 5 cut(s) 385, 2590, 4257, 6397, 6802
BmeRI GACNNNNNGTC 1 cut(s) 6049
BmeT110I CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
BmgBI CACGTC 3 cut(s) 2360, 3128, 3389
BmrI ACTGGG 4 cut(s) 1991, 2277, 5281, 7203
BmtI GCTAGC 2 cut(s) 4413, 7010
BmuI ACTGGG 4 cut(s) 1991, 2277, 5281, 7203
BpiI GAAGAC 3 cut(s) 4959, 5375, 6255
BplI GAGNNNNNCTC 4 cut(s) 4941, 4973, 6041, 6073
BpmI CTGGAG 6 cut(s) 1003, 5936, 6326, 6641, 7038, 7293
Bpu10I CCTNAGC 3 cut(s) 896, 1413, 1776
Bpu1102I GCTNAGC 1 cut(s) 4266
Bpu14I TTCGAA 3 cut(s) 723, 3505, 4462
BpuEI CTTGAG 6 cut(s) 1163, 4109, 4134, 4622, 5625, 6309
BpuMI CCSGG 3 cut(s) 54, 424, 5720
Bsa29I ATCGAT 2 cut(s) 943, 4614
BsaAI YACGTR 4 cut(s) 1106, 1124, 1997, 4487
BsaBI GATNNNNATC 2 cut(s) 3969, 6504
BsaHI GRCGYC 3 cut(s) 72, 1651, 6967
BsaI GGTCTC 2 cut(s) 2663, 6689
BsaWI WCCGGW 3 cut(s) 3240, 6124, 6496
BsaXI ACNNNNNCTCC 6 cut(s) 2097, 2127, 2199, 2229, 6199, 6229
Bse118I RCCGGY 1 cut(s) 7048
Bse21I CCTNAGG 3 cut(s) 23, 860, 1362
Bse8I GATNNNNATC 2 cut(s) 3969, 6504
BseAI TCCGGA 2 cut(s) 3240, 6496
BseBI CCWGG 9 cut(s) 389, 1772, 2436, 2874, 4507, 4829, 5130, 5500, 6102
BseCI ATCGAT 2 cut(s) 943, 4614
BseJI GATNNNNATC 2 cut(s) 3969, 6504
BseRI GAGGAG 4 cut(s) 1679, 4619, 6640, 7207
BseSI GKGCMC 3 cut(s) 39, 3721, 4666
BseYI CCCAGC 4 cut(s) 1856, 3542, 4056, 4261
BsgI GTGCAG 9 cut(s) 35, 85, 1469, 1965, 3188, 3512, 3618, 6536, 6701
Bsh1236I CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
BshVI ATCGAT 2 cut(s) 943, 4614
BsiHKAI GWGCWC 7 cut(s) 268, 2267, 2395, 3883, 4750, 5007, 7090
BsiHKCI CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
BsiSI CCGG 7 cut(s) 54, 423, 3241, 5719, 6125, 6497, 7049
BslFI GGGAC 6 cut(s) 4, 2603, 6255, 6383, 6770, 6788
BsmBI CGTCTC 1 cut(s) 6974
BsmFI GGGAC 6 cut(s) 4, 2603, 6255, 6383, 6770, 6788
BsmI GAATGC 4 cut(s) 316, 458, 1331, 6828
Bso31I GGTCTC 2 cut(s) 2663, 6689
BsoBI CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
Bsp119I TTCGAA 3 cut(s) 723, 3505, 4462
Bsp120I GGGCCC 2 cut(s) 35, 4662
Bsp13I TCCGGA 2 cut(s) 3240, 6496
Bsp1407I TGTACA 3 cut(s) 1612, 2257, 4622
Bsp1720I GCTNAGC 1 cut(s) 4266
Bsp19I CCATGG 2 cut(s) 4897, 5404
Bsp68I TCGCGA 2 cut(s) 1836, 6783
BspDI ATCGAT 2 cut(s) 943, 4614
BspEI TCCGGA 2 cut(s) 3240, 6496
BspFNI CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
BspHI TCATGA 6 cut(s) 822, 4879, 5905, 6657, 6946, 7348
BspMAI CTGCAG 3 cut(s) 442, 4036, 4989
BspMI ACCTGC 2 cut(s) 3160, 4717
BspOI GCTAGC 2 cut(s) 4413, 7010
BspQI GCTCTTC 2 cut(s) 1905, 4221
BspT104I TTCGAA 3 cut(s) 723, 3505, 4462
BspTI CTTAAG 1 cut(s) 172
BspTNI GGTCTC 2 cut(s) 2663, 6689
BsrBI CCGCTC 4 cut(s) 2210, 3083, 4690, 5639
BsrFI RCCGGY 1 cut(s) 7048
BsrGI TGTACA 3 cut(s) 1612, 2257, 4622
BssAI RCCGGY 1 cut(s) 7048
BssNAI GTATAC 1 cut(s) 1286
BssNI GRCGYC 3 cut(s) 72, 1651, 6967
BssSI CACGAG 1 cut(s) 7089
BssT1I CCWWGG 7 cut(s) 117, 664, 3283, 4212, 4897, 5404, 6185
Bst1107I GTATAC 1 cut(s) 1286
Bst2BI CACGAG 1 cut(s) 7089
Bst2UI CCWGG 9 cut(s) 389, 1772, 2436, 2874, 4507, 4829, 5130, 5500, 6102
Bst6I CTCTTC 5 cut(s) 1905, 4221, 4459, 4805, 6977
BstACI GRCGYC 3 cut(s) 72, 1651, 6967
BstAFI CTTAAG 1 cut(s) 172
BstAPI GCANNNNNTGC 8 cut(s) 437, 548, 1802, 3656, 4310, 5279, 5836, 6898
BstAUI TGTACA 3 cut(s) 1612, 2257, 4622
BstBAI YACGTR 4 cut(s) 1106, 1124, 1997, 4487
BstBI TTCGAA 3 cut(s) 723, 3505, 4462
BstDSI CCRYGG 2 cut(s) 4897, 5404
BstEII GGTNACC 1 cut(s) 6671
BstENI CCTNNNNNAGG 5 cut(s) 1775, 3242, 3287, 3649, 4244
BstFNI CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
BstHHI GCGC 4 cut(s) 1796, 3561, 3949, 6258
BstNI CCWGG 9 cut(s) 389, 1772, 2436, 2874, 4507, 4829, 5130, 5500, 6102
BstNSI RCATGY 5 cut(s) 972, 2721, 5456, 5809, 7247
BstPI GGTNACC 1 cut(s) 6671
BstSFI CTRYAG 8 cut(s) 438, 2235, 3858, 3981, 4032, 4985, 5056, 6724
BstSLI GKGCMC 3 cut(s) 39, 3721, 4666
BstSNI TACGTA 1 cut(s) 1997
BstUI CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
BstV2I GAAGAC 3 cut(s) 4959, 5375, 6255
BstX2I RGATCY 7 cut(s) 613, 2125, 2398, 4835, 5983, 6334, 6735
BstXI CCANNNNNNTGG 1 cut(s) 3961
BstYI RGATCY 7 cut(s) 613, 2125, 2398, 4835, 5983, 6334, 6735
BstZ17I GTATAC 1 cut(s) 1286
Bsu15I ATCGAT 2 cut(s) 943, 4614
Bsu36I CCTNAGG 3 cut(s) 23, 860, 1362
BsuI GTATCC 4 cut(s) 95, 2975, 4686, 5496
BsuTUI ATCGAT 2 cut(s) 943, 4614
BtgI CCRYGG 2 cut(s) 4897, 5404
BtgZI GCGATG 3 cut(s) 3323, 4123, 4213
BtrI CACGTC 3 cut(s) 2360, 3128, 3389
BtsI GCAGTG 7 cut(s) 247, 908, 3537, 3809, 4134, 4401, 4746
BtuMI TCGCGA 2 cut(s) 1836, 6783
BveI ACCTGC 2 cut(s) 3160, 4717
CaiI CAGNNNCTG 3 cut(s) 1706, 4591, 5309
CciI TCATGA 6 cut(s) 822, 4879, 5905, 6657, 6946, 7348
CfoI GCGC 4 cut(s) 1796, 3561, 3949, 6258
Cfr10I RCCGGY 1 cut(s) 7048
ClaI ATCGAT 2 cut(s) 943, 4614
CseI GACGC 4 cut(s) 80, 1659, 2990, 6956
CspCI CAANNNNNGTGG 2 cut(s) 1833, 1868
DraI TTTAAA 1 cut(s) 1812
DrdI GACNNNNNNGTC 1 cut(s) 383
DriI GACNNNNNGTC 1 cut(s) 6049
DseDI GACNNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 4 cut(s) 3, 2519, 4341, 7333
Eam1104I CTCTTC 5 cut(s) 1905, 4221, 4459, 4805, 6977
Eam1105I GACNNNNNGTC 1 cut(s) 6049
EarI CTCTTC 5 cut(s) 1905, 4221, 4459, 4805, 6977
Ecl136II GAGCTC 2 cut(s) 266, 5005
Eco105I TACGTA 1 cut(s) 1997
Eco130I CCWWGG 7 cut(s) 117, 664, 3283, 4212, 4897, 5404, 6185
Eco147I AGGCCT 4 cut(s) 3914, 4130, 5897, 6938
Eco24I GRGCYC 5 cut(s) 39, 268, 4666, 5007, 5122
Eco31I GGTCTC 2 cut(s) 2663, 6689
Eco47I GGWCC 5 cut(s) 385, 2590, 4257, 6397, 6802
Eco53kI GAGCTC 2 cut(s) 266, 5005
Eco57I CTGAAG 9 cut(s) 1407, 2559, 3096, 3635, 4671, 4788, 5439, 6257, 7091
Eco72I CACGTG 1 cut(s) 4487
Eco81I CCTNAGG 3 cut(s) 23, 860, 1362
Eco88I CYCGRG 4 cut(s) 3162, 4868, 5840, 7281
Eco91I GGTNACC 1 cut(s) 6671
EcoICRI GAGCTC 2 cut(s) 266, 5005
EcoNI CCTNNNNNAGG 5 cut(s) 1775, 3242, 3287, 3649, 4244
EcoO109I RGGNCCY 6 cut(s) 1184, 1261, 2590, 4663, 6397, 6802
EcoO65I GGTNACC 1 cut(s) 6671
EcoRI GAATTC 3 cut(s) 818, 1766, 6310
EcoRII CCWGG 9 cut(s) 387, 1770, 2434, 2872, 4505, 4827, 5128, 5498, 6100
EcoT14I CCWWGG 7 cut(s) 117, 664, 3283, 4212, 4897, 5404, 6185
EcoT22I ATGCAT 3 cut(s) 316, 1331, 3069
EcoT38I GRGCYC 5 cut(s) 39, 268, 4666, 5007, 5122
ErhI CCWWGG 7 cut(s) 117, 664, 3283, 4212, 4897, 5404, 6185
Esp3I CGTCTC 1 cut(s) 6974
FaqI GGGAC 6 cut(s) 4, 2603, 6255, 6383, 6770, 6788
FauI CCCGC 3 cut(s) 26, 3088, 3675
FauNDI CATATG 1 cut(s) 3063
FbaI TGATCA 2 cut(s) 6505, 7291
FblI GTMKAC 4 cut(s) 1285, 2351, 3418, 7251
FriOI GRGCYC 5 cut(s) 39, 268, 4666, 5007, 5122
GlaI GCGC 4 cut(s) 1795, 3560, 3948, 6257
GsaI CCCAGC 4 cut(s) 1860, 3546, 4060, 4265
GsuI CTGGAG 6 cut(s) 1003, 5936, 6326, 6641, 7038, 7293
HapII CCGG 7 cut(s) 54, 423, 3241, 5719, 6125, 6497, 7049
HgaI GACGC 4 cut(s) 80, 1659, 2990, 6956
HhaI GCGC 4 cut(s) 1796, 3561, 3949, 6258
Hin1I GRCGYC 3 cut(s) 72, 1651, 6967
Hin6I GCGC 4 cut(s) 1794, 3559, 3947, 6256
HinP1I GCGC 4 cut(s) 1794, 3559, 3947, 6256
HincII GTYRAC 6 cut(s) 1926, 2480, 2838, 3419, 4163, 5653
HindII GTYRAC 6 cut(s) 1926, 2480, 2838, 3419, 4163, 5653
HindIII AAGCTT 6 cut(s) 64, 1419, 1679, 2428, 2753, 7187
HpaII CCGG 7 cut(s) 54, 423, 3241, 5719, 6125, 6497, 7049
Hpy99I CGWCG 8 cut(s) 11, 74, 510, 1653, 3390, 3423, 4376, 4662
Hsp92I GRCGYC 3 cut(s) 72, 1651, 6967
HspAI GCGC 4 cut(s) 1794, 3559, 3947, 6256
KflI GGGWCCC 2 cut(s) 2590, 6397
Kpn2I TCCGGA 2 cut(s) 3240, 6496
Ksp22I TGATCA 2 cut(s) 6505, 7291
LguI GCTCTTC 2 cut(s) 1905, 4221
MbiI CCGCTC 4 cut(s) 2210, 3083, 4690, 5639
MfeI CAATTG 1 cut(s) 1113
MflI RGATCY 7 cut(s) 613, 2125, 2398, 4835, 5983, 6334, 6735
MlsI TGGCCA 2 cut(s) 2521, 7335
MluI ACGCGT 1 cut(s) 104
MluNI TGGCCA 2 cut(s) 2521, 7335
MlyI GAGTC 3 cut(s) 1459, 4094, 6059
MmeI TCCRAC 6 cut(s) 772, 1141, 1360, 2605, 4018, 4787
Mox20I TGGCCA 2 cut(s) 2521, 7335
Mph1103I ATGCAT 3 cut(s) 316, 1331, 3069
MroI TCCGGA 2 cut(s) 3240, 6496
MroXI GAANNNNTTC 3 cut(s) 1680, 6789, 7104
MscI TGGCCA 2 cut(s) 2521, 7335
MslI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6207, 6662
Msp20I TGGCCA 2 cut(s) 2521, 7335
MspA1I CMGCKG 6 cut(s) 60, 3698, 4265, 4591, 5240, 6022
MspCI CTTAAG 1 cut(s) 172
MspI CCGG 7 cut(s) 54, 423, 3241, 5719, 6125, 6497, 7049
MunI CAATTG 1 cut(s) 1113
Mva1269I GAATGC 4 cut(s) 316, 458, 1331, 6828
MvaI CCWGG 9 cut(s) 389, 1772, 2436, 2874, 4507, 4829, 5130, 5500, 6102
MvnI CGCG 5 cut(s) 11, 106, 1836, 3949, 6783
NciI CCSGG 3 cut(s) 54, 424, 5720
NcoI CCATGG 2 cut(s) 4897, 5404
NdeI CATATG 1 cut(s) 3063
NheI GCTAGC 2 cut(s) 4409, 7006
NmeAIII GCCGAG 1 cut(s) 5989
NruI TCGCGA 2 cut(s) 1836, 6783
NsiI ATGCAT 3 cut(s) 316, 1331, 3069
NspI RCATGY 5 cut(s) 972, 2721, 5456, 5809, 7247
NspV TTCGAA 3 cut(s) 723, 3505, 4462
OliI CACNNNNGTG 1 cut(s) 1104
PaeI GCATGC 1 cut(s) 5809
PaeR7I CTCGAG 2 cut(s) 5840, 7281
PagI TCATGA 6 cut(s) 822, 4879, 5905, 6657, 6946, 7348
PaqCI CACCTGC 1 cut(s) 4717
PceI AGGCCT 4 cut(s) 3914, 4130, 5897, 6938
PciI ACATGT 3 cut(s) 968, 2717, 5452
PciSI GCTCTTC 2 cut(s) 1905, 4221
PcsI WCGNNNNNNNCGW 6 cut(s) 36, 688, 1129, 1645, 4371, 7087
PctI GAATGC 4 cut(s) 316, 458, 1331, 6828
PdmI GAANNNNTTC 3 cut(s) 1680, 6789, 7104
PfeI GAWTC 9 cut(s) 976, 1517, 2471, 3620, 4639, 5027, 5144, 6119, 6778
PflFI GACNNNGTC 3 cut(s) 1653, 3251, 5565
PflMI CCANNNNNTGG 4 cut(s) 1379, 2494, 3677, 6380
PfoI TCCNGGA 1 cut(s) 5128
PleI GAGTC 3 cut(s) 1459, 4093, 6058
PmaCI CACGTG 1 cut(s) 4487
PmlI CACGTG 1 cut(s) 4487
PpsI GAGTC 3 cut(s) 1459, 4093, 6058
Ppu21I YACGTR 4 cut(s) 1106, 1124, 1997, 4487
PpuMI RGGWCCY 3 cut(s) 2590, 6397, 6802
PscI ACATGT 3 cut(s) 968, 2717, 5452
PshBI ATTAAT 1 cut(s) 5463
Psp124BI GAGCTC 2 cut(s) 268, 5007
Psp5II RGGWCCY 3 cut(s) 2590, 6397, 6802
Psp6I CCWGG 9 cut(s) 387, 1770, 2434, 2872, 4505, 4827, 5128, 5498, 6100
PspCI CACGTG 1 cut(s) 4487
PspEI GGTNACC 1 cut(s) 6671
PspFI CCCAGC 4 cut(s) 1856, 3542, 4056, 4261
PspGI CCWGG 9 cut(s) 387, 1770, 2434, 2872, 4505, 4827, 5128, 5498, 6100
PspOMI GGGCCC 2 cut(s) 35, 4662
PspPPI RGGWCCY 3 cut(s) 2590, 6397, 6802
PspXI VCTCGAGB 1 cut(s) 5840
PsrI GAACNNNNNNTAC 2 cut(s) 2358, 2390
PstI CTGCAG 3 cut(s) 442, 4036, 4989
PstNI CAGNNNCTG 3 cut(s) 1706, 4591, 5309
PsuI RGATCY 7 cut(s) 613, 2125, 2398, 4835, 5983, 6334, 6735
PsyI GACNNNGTC 3 cut(s) 1653, 3251, 5565
PvuII CAGCTG 5 cut(s) 3698, 4265, 4591, 5240, 6022
RruI TCGCGA 2 cut(s) 1836, 6783
RseI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6207, 6662
SacI GAGCTC 2 cut(s) 268, 5007
SalI GTCGAC 1 cut(s) 3417
SapI GCTCTTC 2 cut(s) 1905, 4221
ScaI AGTACT 2 cut(s) 2367, 5062
SchI GAGTC 3 cut(s) 1459, 4094, 6059
SfcI CTRYAG 8 cut(s) 438, 2235, 3858, 3981, 4032, 4985, 5056, 6724
Sfr274I CTCGAG 2 cut(s) 5840, 7281
SfuI TTCGAA 3 cut(s) 723, 3505, 4462
SinI GGWCC 5 cut(s) 385, 2590, 4257, 6397, 6802
SlaI CTCGAG 2 cut(s) 5840, 7281
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6207, 6662
SmlI CTYRAG 9 cut(s) 172, 1142, 4124, 4149, 4601, 5640, 5840, 6324, 7281
SmoI CTYRAG 9 cut(s) 172, 1142, 4124, 4149, 4601, 5640, 5840, 6324, 7281
SnaBI TACGTA 1 cut(s) 1997
SpeI ACTAGT 1 cut(s) 3734
SphI GCATGC 1 cut(s) 5809
SseBI AGGCCT 4 cut(s) 3914, 4130, 5897, 6938
SspI AATATT 5 cut(s) 1168, 1731, 3787, 4044, 5461
SstI GAGCTC 2 cut(s) 268, 5007
StuI AGGCCT 4 cut(s) 3914, 4130, 5897, 6938
StyI CCWWGG 7 cut(s) 117, 664, 3283, 4212, 4897, 5404, 6185
TaqII GACCGA 1 cut(s) 2237
TatI WGTACW 6 cut(s) 1612, 2257, 2365, 4622, 5060, 6435
TauI GCSGC 5 cut(s) 50, 1908, 2213, 2782, 5639
TfiI GAWTC 9 cut(s) 976, 1517, 2471, 3620, 4639, 5027, 5144, 6119, 6778
TspGWI ACGGA 6 cut(s) 1563, 2274, 2806, 2852, 3080, 6857
Tth111I GACNNNGTC 3 cut(s) 1653, 3251, 5565
Van91I CCANNNNNTGG 4 cut(s) 1379, 2494, 3677, 6380
Vha464I CTTAAG 1 cut(s) 172
VpaK11BI GGWCC 5 cut(s) 385, 2590, 4257, 6397, 6802
VspI ATTAAT 1 cut(s) 5463
XagI CCTNNNNNAGG 5 cut(s) 1775, 3242, 3287, 3649, 4244
XbaI TCTAGA 2 cut(s) 2395, 6314
XceI RCATGY 5 cut(s) 972, 2721, 5456, 5809, 7247
XcmI CCANNNNNNNNNTGG 3 cut(s) 4513, 4943, 7079
XhoI CTCGAG 2 cut(s) 5840, 7281
XmiI GTMKAC 4 cut(s) 1285, 2351, 3418, 7251
XmnI GAANNNNTTC 3 cut(s) 1680, 6789, 7104
ZrmI AGTACT 2 cut(s) 2367, 5062
Zsp2I ATGCAT 3 cut(s) 316, 1331, 3069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.