MD10G1112000.v1.1

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0005708
Physical Location & Seq
Reverse (-)
18532054 .. 18545008
12955 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1112000.v1.1.491

Sequence Viewer

Length: 3966 bp
ATGGCGGCCTCGGGGCAGTCCCTCAGGTTCTGCGGCCCCGGCTCCGCCGGACCCAGTGGAGGAAGTTTTGACGCGCTCAATCGCATCCTTGCTGACCTCTGCACCCGTGGCAGCCCCAAGGATGGGGCTTCGTTAGCATTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACCTCAGCGGAGAAGCATTTTCGCGTTTCATGGATCAGTTGTATGATCGAATTTCATCTCTACTGGAGAGCAGTGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGATGAGTTGATAGATGTGGCACTGGGAGAGAATTCCTCCAAGGTTTCGAAGTTTGCTAACTACATACGGACTGTTTTTGAGGTAAAGCGTGATCCGGATATTCTGGTGCTTGCTAGTAGAGTTCTGGGCCATCTTGCTAGGGCAGGGGGAGCAATGACTGCAGATGAAGTGGAACGCCAGATAAAAATTGCTCTGGATTGGTTGCGGGGAGATAGAGTTGAGTATCGCCGTTTTGCTGCTGTCTTGATTTTGAAAGAAATGGCAGAAAACGCTTCAACAGTGTTCAATGTTCATGTGCCTGAATTTGTTGATGCTATTTGGGTTGCTTTGAGGGATCCAGTGTTGCCTATTCGAGAGCGAGCTGTGGAAGCTCTACGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGCTGGCGTGTGCAATGGTATTATCGTATGTTTGAGGCTACCCAAGATGGGTTGGGTAGAAATGCTTCCATTCACAGCATACATGGTTCTTTGCTTGCAGTTGGAGAACTTTTAAGGAATACAGGTGAATTTATGATGTCTAGGTACAGGGAAGTTGCCGAAATTGTCCTTAGATATCTTGAGCACCGGGACAGGCTGGTTCGCCTGAGCATAACTTCGCTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTCACAAACTACTTAACGATATGTATGAATCATATTCTTGCAGTTTTGCGAATACCGGCAGAACGCTCCAGTGGTTTTATTGCCCTTGGGGAGATGGCTGGTGCTTTGGACGGGGAACTTGTCCATTATTTGCCGACAATAACGTCTCATTTACGTGATGCGATTGCTCCACGTAGAGGTAGACCCTCTCTTGAGGCTTTGGTTTGTGTTGGAAACATAGCAAAAGCAATGGGGCCGGCTATGGAACCTCATGTTCTTGGTCTTTTGGATGTTATGTTCTCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCACTTGAGCAAATAACTACCAGTATTACATCTTTGCTTCCTACCATCCAAGATCGGTTACTTGACTGCATTTCGGCGGTTCTTTCAAAATCCTACCAGTCTCAGGGAAGGTCGGCTGTTGGCATGGGTAGAGGAAATTTAATGAATGTCCCTCAGCCAGCGTCAGACCTCAGTGGTTCAGCCCTTGTGCAGCTTGCTTTGCAGACTTTAGCTCGTTTTAATTTCAAGGGCCATGACCTTCTTGAGTTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGATGGAGCCATCCGGAAAGATGCTGCCCTATGTTGTTGCAGATTAGTTGCAAATTCCTTTTCTGGTATGCAATATGCATCTGGTAGGGCAAATCGGGGAAAGCGACGACGTCTTATTGAGGAGATTGTGGAAAAGCTTCTCAGTGAAGCTGTTGCAGATGCTGATGTTATTGTTCGTCATTCAATATTTTCCTCCCTTCATGGGAATAGAGGTTTTGATGATTTTTTGGCGCAGGCTGATAGTCTGAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGAGGTTCGAGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAACCCTGCTTATGTTCTTCCAGCACTTCGTCGGCATCTTATACAGTTGTTGACTTACCTGGGGCAGAGTGCAGATACCAAATGCCGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGAAACTGTGAACGACTAATACTTCCATACATTGCTCCGATACACAAGGCACTTGTTGCAAGACTTAATGATGGCACTGGTGTGAACGCAAATAATGGCATTATTAGTGGAGTTCTTGTAACTGTTGGGGATCTGGCTAGAGTGGGTGGTTTTGCAATGCGAAAATACATTCCTGAGCTTATGCCTTTAATTGTTGATGCTTTATTGGATGGAGCAGCCGTCACCAAACGGGAAGTGGCTGTCGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCATACAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTACTGAATGGTGAATTAGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTCGGAATTATGGGCGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCCTGCCTGGGTCACATGGAGATGTTACCCGTAGTGCGAGTGAATCTGGTCAACACTTTCAGTCTGTGGATGAATTGCCTATGGACCTTTGGCCATCATTTGCAACCTCAGAAGACTATTATTCTACGGTTGCAATCAACTCGCTCATGCGTATACTTAGGGACCCTTCACTTGCTACTTACCACCTAAAGGTGGTCGGATCTCTTATGTTCATATTCAAGTCAATGGGTCTTGGTTGTGTTCCATACTTACCAAAGGTTTTACCTGATCTCTTACATATAGTCCGGACATGTGATGATGCTTTAAAGGATTTTATAACATGGAAGCTTGGCACACTAGTGTCTATTGTGCGTCAGCATATTCGGAAATATCTGCATGAGTTGCTCGTTCTAATTTCAGAACTATGGTCAACCTTCAGTTTTCCTGCTGCTGGCCGTCCTCAACTTGGTTATCCAGTTCTGCATCTGGTGGAGCAGCTTTGCTTGGCTCTCAATGATGAATTTCGAACATACCTTCCTGATATTCTTCCATGTTGTATTCAAGTTCTAAGTGATGCAGAACGGTATAACGATTATACTTATGTTCTGGACATCCTCCGTACCCTTGAAGTGTTTGGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCTGCACTCATTCGCTTGTTTAAAGTTGATGCTTCAGTGGACATAAGACGTGCTGCTATTAAAACTTTGACGAAACTTATTCCTCGTGTGCAGGTTGCTGGTCACATATCTTCTCTCGTGCATCACTTAAAGCTAGTGTTAGATGGCAAGAACGATGAGCTCCGGAAGGATGCTGTTGATGCCCTTTGTTGTCTAGCTCATGCTCTTGGGGAGGACTTCACAATTTTTATCCCATCAATACACAAACTTCTGCTGAAACATCGTTTAAGGCATAAGGAATTTGAGGAAATTGAAGGGCGGTTGAAGCGACGTGAACCTCTAATTTTGGGGAGTACAACTTTTCAAAGATTAAGCAGACGGCTTCCAGTAGAGGTTATTACTGACCGTTGGAATGATTTAGAGATTGACCTTTATGATAATGGGTCAGATATGCAGAAACAACTTCGAGGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACATTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCCCTACGAACCTGTGCAAGGCTTGCACAGTTGCAGCCATTTGTTGGGCGGGAGCTATTTGCAGCTGGTTTTGTCAGCTGTTGGGCACAGCTGAACGAGACTAGTCAGAAACAGTTAGTTCGAAGTTTGGAGATGGCATTTTCATCCCCAAATATACCTCCTGAAATTTTGGCAACACTTCTTAATTTGGTATGTCTTTTTCATTATATTATAACTGGACATGAATCATGTGATTTTAGCTTGGAATGCTTAAAGCCTCTGTTGCATGATTACATTTACATATTTGTATGTACGAATGTATATAGTTTTCTCTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

1322

Amino Acids

147.23

Weight (kDa)

6.59

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 758 - 926 7.6e-53 Serine/threonine-protein kinase mTOR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2741, 3860
AasI GACNNNNNNGTC 1 cut(s) 1090
AatII GACGTC 1 cut(s) 1654
Acc36I ACCTGC 1 cut(s) 3157
AccB7I CCANNNNNTGG 1 cut(s) 3692
AccI GTMKAC 3 cut(s) 1129, 2348, 2578
AccII CGCG 2 cut(s) 74, 193
AccIII TCCGGA 4 cut(s) 373, 1554, 2709, 3237
AciI CCGC 8 cut(s) 5, 33, 45, 177, 484, 1334, 3373, 3697
AclWI GGATC 7 cut(s) 210, 365, 608, 621, 2130, 2390, 2632
AcoI YGGCCR 2 cut(s) 2516, 2857
AcuI CTGAAG 3 cut(s) 2824, 3093, 3650
AcyI GRCGYC 1 cut(s) 1651
AfaI GTAC 7 cut(s) 836, 2256, 2364, 3025, 3409, 3580, 3940
AflIII ACRYGT 1 cut(s) 2714
AhlI ACTAGT 2 cut(s) 2761, 3749
AjiI CACGTC 3 cut(s) 2357, 3125, 3386
AjnI CCWGG 2 cut(s) 1929, 2431
AleI CACNNNNGTG 2 cut(s) 2072, 2762
AloI GAACNNNNNNTCC 4 cut(s) 1208, 1240, 2923, 2955
Alw21I GWGCWC 4 cut(s) 268, 876, 2264, 3237
Alw26I GTCTC 5 cut(s) 680, 1098, 1362, 3601, 3740
AlwI GGATC 7 cut(s) 210, 365, 608, 621, 2130, 2390, 2632
AlwNI CAGNNNCTG 2 cut(s) 30, 1703
Ama87I CYCGRG 1 cut(s) 10
Aor13HI TCCGGA 4 cut(s) 373, 1554, 2709, 3237
AoxI GGCC 8 cut(s) 6, 34, 406, 1181, 1486, 2516, 2857, 3523
Asp700I GAANNNNTTC 2 cut(s) 754, 1677
AspLEI GCGC 5 cut(s) 76, 1774, 1793, 2390, 3576
AspS9I GGNCC 7 cut(s) 35, 50, 406, 1181, 1486, 2509, 2587
AsuC2I CCSGG 3 cut(s) 39, 878, 1958
AsuHPI GGTGA 3 cut(s) 827, 2206, 2345
AsuII TTCGAA 3 cut(s) 326, 2929, 3769
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 3 cut(s) 50, 2509, 2587
AxyI CCTNAGG 1 cut(s) 23
BaeGI GKGCMC 1 cut(s) 3736
BalI TGGCCA 1 cut(s) 2518
BamHI GGATCC 1 cut(s) 613
BanII GRGCYC 2 cut(s) 268, 3237
BarI GAAGNNNNNNTAC 2 cut(s) 739, 771
BauI CACGAG 2 cut(s) 3159, 3191
BbsI GAAGAC 1 cut(s) 2544
Bbv12I GWGCWC 4 cut(s) 268, 876, 2264, 3237
BbvCI CCTCAGC 2 cut(s) 173, 1410
BceAI ACGGC 4 cut(s) 492, 2195, 2844, 3449
BcgI CGANNNNNNTGC 2 cut(s) 2547, 2581
BciT130I CCWGG 2 cut(s) 1931, 2433
BcnI CCSGG 3 cut(s) 39, 878, 1958
BcoDI GTCTC 5 cut(s) 680, 1098, 1362, 3601, 3740
BcuI ACTAGT 2 cut(s) 2761, 3749
BfaI CTAG 9 cut(s) 393, 417, 831, 2130, 2393, 2762, 3209, 3269, 3750
BfmI CTRYAG 1 cut(s) 438
BfoI RGCGCY 1 cut(s) 2391
BfuAI ACCTGC 1 cut(s) 3157
BmcAI AGTACT 1 cut(s) 2364
Bme1390I CCNGG 5 cut(s) 39, 878, 1931, 1958, 2433
Bme18I GGWCC 3 cut(s) 50, 2509, 2587
BmeT110I CYCGRG 1 cut(s) 10
BmgBI CACGTC 3 cut(s) 2357, 3125, 3386
BmgT120I GGNCC 7 cut(s) 35, 50, 406, 1181, 1486, 2509, 2587
BmiI GGNNCC 9 cut(s) 37, 43, 52, 615, 1182, 1194, 1549, 2588, 2589
BmrFI CCNGG 5 cut(s) 39, 878, 1931, 1958, 2433
BmrI ACTGGG 4 cut(s) 48, 311, 1988, 2274
BmuI ACTGGG 4 cut(s) 48, 311, 1988, 2274
BpiI GAAGAC 1 cut(s) 2544
BplI GAGNNNNNCTC 2 cut(s) 299, 331
BpmI CTGGAG 2 cut(s) 254, 1000
Bpu10I CCTNAGC 4 cut(s) 173, 896, 1410, 2166
Bpu14I TTCGAA 3 cut(s) 326, 2929, 3769
BpuEI CTTGAG 4 cut(s) 890, 1160, 1283, 1520
BpuMI CCSGG 3 cut(s) 39, 878, 1958
Bsa29I ATCGAT 1 cut(s) 943
BsaAI YACGTR 3 cut(s) 656, 1103, 1121
BsaHI GRCGYC 1 cut(s) 1651
BsaJI CCNNGG 9 cut(s) 9, 37, 106, 117, 318, 664, 1033, 1930, 2432
BsaWI WCCGGW 4 cut(s) 373, 1554, 2709, 3237
BsaXI ACNNNNNCTCC 6 cut(s) 480, 510, 2094, 2124, 2196, 2226
Bse118I RCCGGY 2 cut(s) 1003, 1183
Bse21I CCTNAGG 1 cut(s) 23
Bse3DI GCAATG 5 cut(s) 438, 710, 1182, 2022, 2154
BseAI TCCGGA 4 cut(s) 373, 1554, 2709, 3237
BseBI CCWGG 2 cut(s) 1931, 2433
BseCI ATCGAT 1 cut(s) 943
BseDI CCNNGG 9 cut(s) 9, 37, 106, 117, 318, 664, 1033, 1930, 2432
BseMI GCAATG 5 cut(s) 438, 710, 1182, 2022, 2154
BseRI GAGGAG 1 cut(s) 1676
BseSI GKGCMC 1 cut(s) 3736
BseYI CCCAGC 2 cut(s) 1853, 3557
BsgI GTGCAG 5 cut(s) 85, 1466, 1962, 3063, 3185
Bsh1236I CGCG 2 cut(s) 74, 193
BshFI GGCC 8 cut(s) 8, 36, 408, 1183, 1488, 2518, 2859, 3525
BshVI ATCGAT 1 cut(s) 943
BsiHKAI GWGCWC 4 cut(s) 268, 876, 2264, 3237
BsiHKCI CYCGRG 1 cut(s) 10
BslFI GGGAC 4 cut(s) 4, 893, 1391, 2600
BsmAI GTCTC 5 cut(s) 680, 1098, 1362, 3601, 3740
BsmBI CGTCTC 1 cut(s) 1098
BsmFI GGGAC 4 cut(s) 4, 893, 1391, 2600
BsmI GAATGC 1 cut(s) 3899
BsnI GGCC 8 cut(s) 8, 36, 408, 1183, 1488, 2518, 2859, 3525
BsoBI CYCGRG 1 cut(s) 10
Bsp119I TTCGAA 3 cut(s) 326, 2929, 3769
Bsp1286I GDGCHC 5 cut(s) 268, 876, 2264, 3237, 3736
Bsp13I TCCGGA 4 cut(s) 373, 1554, 2709, 3237
Bsp1407I TGTACA 1 cut(s) 2254
BspACI CCGC 8 cut(s) 5, 33, 45, 177, 484, 1334, 3373, 3697
BspANI GGCC 8 cut(s) 8, 36, 408, 1183, 1488, 2518, 2859, 3525
BspDI ATCGAT 1 cut(s) 943
BspEI TCCGGA 4 cut(s) 373, 1554, 2709, 3237
BspFNI CGCG 2 cut(s) 74, 193
BspLI GGNNCC 9 cut(s) 37, 43, 52, 615, 1182, 1194, 1549, 2588, 2589
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 1 cut(s) 3157
BspPI GGATC 7 cut(s) 210, 365, 608, 621, 2130, 2390, 2632
BspT104I TTCGAA 3 cut(s) 326, 2929, 3769
BsrDI GCAATG 5 cut(s) 438, 710, 1182, 2022, 2154
BsrFI RCCGGY 2 cut(s) 1003, 1183
BsrGI TGTACA 1 cut(s) 2254
BssAI RCCGGY 2 cut(s) 1003, 1183
BssECI CCNNGG 9 cut(s) 9, 37, 106, 117, 318, 664, 1033, 1930, 2432
BssNAI GTATAC 1 cut(s) 2579
BssNI GRCGYC 1 cut(s) 1651
BssSI CACGAG 2 cut(s) 3159, 3191
BssT1I CCWWGG 4 cut(s) 117, 318, 664, 1033
Bst1107I GTATAC 1 cut(s) 2579
Bst2BI CACGAG 2 cut(s) 3159, 3191
Bst2UI CCWGG 2 cut(s) 1931, 2433
BstACI GRCGYC 1 cut(s) 1651
BstAPI GCANNNNNTGC 6 cut(s) 437, 1799, 2048, 2428, 2806, 3671
BstAUI TGTACA 1 cut(s) 2254
BstBAI YACGTR 3 cut(s) 656, 1103, 1121
BstBI TTCGAA 3 cut(s) 326, 2929, 3769
BstDSI CCRYGG 1 cut(s) 106
BstENI CCTNNNNNAGG 1 cut(s) 3664
BstFNI CGCG 2 cut(s) 74, 193
BstH2I RGCGCY 1 cut(s) 2391
BstHHI GCGC 5 cut(s) 76, 1774, 1793, 2390, 3576
BstMAI GTCTC 5 cut(s) 680, 1098, 1362, 3601, 3740
BstNI CCWGG 2 cut(s) 1931, 2433
BstNSI RCATGY 1 cut(s) 2718
BstSCI CCNGG 5 cut(s) 37, 876, 1929, 1956, 2431
BstSFI CTRYAG 1 cut(s) 438
BstSLI GKGCMC 1 cut(s) 3736
BstUI CGCG 2 cut(s) 74, 193
BstV2I GAAGAC 1 cut(s) 2544
BstX2I RGATCY 4 cut(s) 613, 2122, 2395, 2624
BstYI RGATCY 4 cut(s) 613, 2122, 2395, 2624
BstZ17I GTATAC 1 cut(s) 2579
Bsu15I ATCGAT 1 cut(s) 943
Bsu36I CCTNAGG 1 cut(s) 23
BsuRI GGCC 8 cut(s) 8, 36, 408, 1183, 1488, 2518, 2859, 3525
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 1 cut(s) 106
BtrI CACGTC 3 cut(s) 2357, 3125, 3386
BtsI GCAGTG 2 cut(s) 247, 3552
BveI ACCTGC 1 cut(s) 3157
CaiI CAGNNNCTG 2 cut(s) 30, 1703
CfoI GCGC 5 cut(s) 76, 1774, 1793, 2390, 3576
Cfr10I RCCGGY 2 cut(s) 1003, 1183
Cfr13I GGNCC 7 cut(s) 35, 50, 406, 1181, 1486, 2509, 2587
ClaI ATCGAT 1 cut(s) 943
CseI GACGC 3 cut(s) 80, 1407, 2765
Csp6I GTAC 7 cut(s) 835, 2255, 2363, 3024, 3408, 3579, 3939
CspCI CAANNNNNGTGG 2 cut(s) 1830, 1865
CviQI GTAC 7 cut(s) 835, 2255, 2363, 3024, 3408, 3579, 3939
DraI TTTAAA 3 cut(s) 1809, 2730, 3097
DrdI GACNNNNNNGTC 1 cut(s) 1090
DseDI GACNNNNNNGTC 1 cut(s) 1090
EaeI YGGCCR 2 cut(s) 2516, 2857
EciI GGCGGA 1 cut(s) 34
Ecl136II GAGCTC 2 cut(s) 266, 3235
Eco130I CCWWGG 4 cut(s) 117, 318, 664, 1033
Eco24I GRGCYC 2 cut(s) 268, 3237
Eco32I GATATC 1 cut(s) 866
Eco47I GGWCC 3 cut(s) 50, 2509, 2587
Eco53kI GAGCTC 2 cut(s) 266, 3235
Eco57I CTGAAG 3 cut(s) 2824, 3093, 3650
Eco81I CCTNAGG 1 cut(s) 23
Eco88I CYCGRG 1 cut(s) 10
EcoICRI GAGCTC 2 cut(s) 266, 3235
EcoNI CCTNNNNNAGG 1 cut(s) 3664
EcoO109I RGGNCCY 1 cut(s) 2587
EcoRI GAATTC 1 cut(s) 310
EcoRII CCWGG 2 cut(s) 1929, 2431
EcoRV GATATC 1 cut(s) 866
EcoT14I CCWWGG 4 cut(s) 117, 318, 664, 1033
EcoT22I ATGCAT 2 cut(s) 1621, 3066
EcoT38I GRGCYC 2 cut(s) 268, 3237
ErhI CCWWGG 4 cut(s) 117, 318, 664, 1033
Esp3I CGTCTC 1 cut(s) 1098
FaqI GGGAC 4 cut(s) 4, 893, 1391, 2600
FauI CCCGC 2 cut(s) 477, 3690
FauNDI CATATG 1 cut(s) 3060
FblI GTMKAC 3 cut(s) 1129, 2348, 2578
FriOI GRGCYC 2 cut(s) 268, 3237
FspBI CTAG 9 cut(s) 393, 417, 831, 2130, 2393, 2762, 3209, 3269, 3750
GlaI GCGC 5 cut(s) 75, 1773, 1792, 2389, 3575
GsaI CCCAGC 2 cut(s) 1857, 3561
GsuI CTGGAG 2 cut(s) 254, 1000
HaeII RGCGCY 1 cut(s) 2391
HaeIII GGCC 8 cut(s) 8, 36, 408, 1183, 1488, 2518, 2859, 3525
HgaI GACGC 3 cut(s) 80, 1407, 2765
HhaI GCGC 5 cut(s) 76, 1774, 1793, 2390, 3576
Hin1I GRCGYC 1 cut(s) 1651
Hin6I GCGC 5 cut(s) 74, 1772, 1791, 2388, 3574
HinP1I GCGC 5 cut(s) 74, 1772, 1791, 2388, 3574
HincII GTYRAC 3 cut(s) 1923, 2477, 2835
HindII GTYRAC 3 cut(s) 1923, 2477, 2835
HindIII AAGCTT 2 cut(s) 1676, 2750
HinfI GANTC 5 cut(s) 976, 1514, 2468, 3635, 3872
HphI GGTGA 3 cut(s) 827, 2206, 2345
Hpy99I CGWCG 4 cut(s) 1650, 1653, 1905, 3387
HpyCH4IV ACGT 9 cut(s) 655, 682, 1091, 1102, 1120, 1651, 2356, 3124, 3385
HpySE526I ACGT 9 cut(s) 655, 682, 1091, 1102, 1120, 1651, 2356, 3124, 3385
Hsp92I GRCGYC 1 cut(s) 1651
HspAI GCGC 5 cut(s) 74, 1772, 1791, 2388, 3574
KflI GGGWCCC 1 cut(s) 2587
Kpn2I TCCGGA 4 cut(s) 373, 1554, 2709, 3237
KroI GCCGGC 1 cut(s) 1183
KroNI GCCGGC 1 cut(s) 1185
MaeI CTAG 9 cut(s) 393, 417, 831, 2130, 2393, 2762, 3209, 3269, 3750
MaeII ACGT 9 cut(s) 655, 682, 1091, 1102, 1120, 1651, 2356, 3124, 3385
MflI RGATCY 4 cut(s) 613, 2122, 2395, 2624
MhlI GDGCHC 5 cut(s) 268, 876, 2264, 3237, 3736
MlsI TGGCCA 1 cut(s) 2518
MluNI TGGCCA 1 cut(s) 2518
MmeI TCCRAC 4 cut(s) 772, 1138, 2602, 3443
Mox20I TGGCCA 1 cut(s) 2518
Mph1103I ATGCAT 2 cut(s) 1621, 3066
MroI TCCGGA 4 cut(s) 373, 1554, 2709, 3237
MroNI GCCGGC 1 cut(s) 1183
MroXI GAANNNNTTC 2 cut(s) 754, 1677
MscI TGGCCA 1 cut(s) 2518
MslI CAYNNNNRTG 4 cut(s) 1101, 2072, 2445, 2762
Msp20I TGGCCA 1 cut(s) 2518
MspA1I CMGCKG 4 cut(s) 177, 3713, 3726, 3739
MspR9I CCNGG 5 cut(s) 39, 878, 1931, 1958, 2433
Mva1269I GAATGC 1 cut(s) 3899
MvaI CCWGG 2 cut(s) 1931, 2433
MvnI CGCG 2 cut(s) 74, 193
NaeI GCCGGC 1 cut(s) 1185
NciI CCSGG 3 cut(s) 39, 878, 1958
NdeI CATATG 1 cut(s) 3060
NgoMIV GCCGGC 1 cut(s) 1183
NlaIV GGNNCC 9 cut(s) 37, 43, 52, 615, 1182, 1194, 1549, 2588, 2589
NmuCI GTSAC 5 cut(s) 167, 949, 2212, 2436, 3176
NsiI ATGCAT 2 cut(s) 1621, 3066
NspI RCATGY 1 cut(s) 2718
NspV TTCGAA 3 cut(s) 326, 2929, 3769
OliI CACNNNNGTG 2 cut(s) 2072, 2762
PciI ACATGT 1 cut(s) 2714
PcsI WCGNNNNNNNCGW 1 cut(s) 1642
PctI GAATGC 1 cut(s) 3899
PdiI GCCGGC 1 cut(s) 1185
PdmI GAANNNNTTC 2 cut(s) 754, 1677
PfeI GAWTC 5 cut(s) 976, 1514, 2468, 3635, 3872
PflFI GACNNNGTC 1 cut(s) 1650
PflMI CCANNNNNTGG 1 cut(s) 3692
Ppu21I YACGTR 3 cut(s) 656, 1103, 1121
PpuMI RGGWCCY 1 cut(s) 2587
PscI ACATGT 1 cut(s) 2714
PsiI TTATAA 2 cut(s) 2741, 3860
Psp124BI GAGCTC 2 cut(s) 268, 3237
Psp5II RGGWCCY 1 cut(s) 2587
Psp6I CCWGG 2 cut(s) 1929, 2431
PspFI CCCAGC 2 cut(s) 1853, 3557
PspGI CCWGG 2 cut(s) 1929, 2431
PspN4I GGNNCC 9 cut(s) 37, 43, 52, 615, 1182, 1194, 1549, 2588, 2589
PspPI GGNCC 7 cut(s) 35, 50, 406, 1181, 1486, 2509, 2587
PspPPI RGGWCCY 1 cut(s) 2587
PsrI GAACNNNNNNTAC 4 cut(s) 1995, 2027, 2355, 2387
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 2 cut(s) 30, 1703
PsuI RGATCY 4 cut(s) 613, 2122, 2395, 2624
PsyI GACNNNGTC 1 cut(s) 1650
PvuII CAGCTG 3 cut(s) 3713, 3726, 3739
RsaI GTAC 7 cut(s) 836, 2256, 2364, 3025, 3409, 3580, 3940
RsaNI GTAC 7 cut(s) 835, 2255, 2363, 3024, 3408, 3579, 3939
RseI CAYNNNNRTG 4 cut(s) 1101, 2072, 2445, 2762
SacI GAGCTC 2 cut(s) 268, 3237
Sau96I GGNCC 7 cut(s) 35, 50, 406, 1181, 1486, 2509, 2587
ScaI AGTACT 1 cut(s) 2364
ScrFI CCNGG 5 cut(s) 39, 878, 1931, 1958, 2433
SduI GDGCHC 5 cut(s) 268, 876, 2264, 3237, 3736
SfcI CTRYAG 1 cut(s) 438
SfuI TTCGAA 3 cut(s) 326, 2929, 3769
SinI GGWCC 3 cut(s) 50, 2509, 2587
SmiMI CAYNNNNRTG 4 cut(s) 1101, 2072, 2445, 2762
SmlI CTYRAG 4 cut(s) 869, 1139, 1262, 1499
SmoI CTYRAG 4 cut(s) 869, 1139, 1262, 1499
SpeI ACTAGT 2 cut(s) 2761, 3749
SsiI CCGC 8 cut(s) 5, 33, 45, 177, 484, 1334, 3373, 3697
SspI AATATT 1 cut(s) 1728
SspMI CTAG 9 cut(s) 393, 417, 831, 2130, 2393, 2762, 3209, 3269, 3750
SstI GAGCTC 2 cut(s) 268, 3237
StyD4I CCNGG 5 cut(s) 37, 876, 1929, 1956, 2431
StyI CCWWGG 4 cut(s) 117, 318, 664, 1033
TaiI ACGT 9 cut(s) 658, 685, 1094, 1105, 1123, 1654, 2359, 3127, 3388
TatI WGTACW 3 cut(s) 2254, 2362, 3407
TauI GCSGC 2 cut(s) 8, 36
TfiI GAWTC 5 cut(s) 976, 1514, 2468, 3635, 3872
TseFI GTSAC 5 cut(s) 167, 949, 2212, 2436, 3176
Tsp45I GTSAC 5 cut(s) 167, 949, 2212, 2436, 3176
TspGWI ACGGA 2 cut(s) 361, 3011
Tth111I GACNNNGTC 1 cut(s) 1650
Van91I CCANNNNNTGG 1 cut(s) 3692
VpaK11BI GGWCC 3 cut(s) 50, 2509, 2587
XagI CCTNNNNNAGG 1 cut(s) 3664
XbaI TCTAGA 1 cut(s) 2392
XceI RCATGY 1 cut(s) 2718
XcmI CCANNNNNNNNNTGG 1 cut(s) 2224
XmiI GTMKAC 3 cut(s) 1129, 2348, 2578
XmnI GAANNNNTTC 2 cut(s) 754, 1677
XspI CTAG 9 cut(s) 393, 417, 831, 2130, 2393, 2762, 3209, 3269, 3750
ZraI GACGTC 1 cut(s) 1652
ZrmI AGTACT 1 cut(s) 2364
Zsp2I ATGCAT 2 cut(s) 1621, 3066
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.