Rroxscaffold_7G00213560

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0005708
Physical Location & Seq
Reverse (-)
64033043 .. 64058290
25248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00213560.1

Sequence Viewer

Length: 7434 bp
ATGGCCGTCTCGGTGCAGTCCCTCAGGTTCGGTGGCCCCGTCACCGCCGCACCCGGCGGCGGAAGCTTCGACGCCCTCAACCGTATCCTCGCCGACCTCTGCACGCGTGGCAACCCCAAGGAGGGGGCTTCGTTGGCTTTGAAGAAGCATCTGGAAGAAGAAGCCCGTGACTTAAATGGAGAAGCATTTTCTCGTTTCATGGATCAGTTGTATGATCGGATTTCTGCTCTTTTGGATAGCGGCGATGTTGCAGAAAATTTGGGAGCTCTAAGAGCTATTGACGAGTTGATAGATGTGGCACTTGGAGAGAATGCATCAAAGGTTTCAAAGTTTGCTAATTACATAAGGACTGCCTTTGAGCTAAAACGTGATCCTGACATTTTGGTCCTGGCTAGTAGAGTTTTGGGCCATCTAGCTAGGGCCGGGGGAGCAATGACTGCAGATGAAGTGGAATGCCAGATAAAAATTGCTCTGGAATGGTTGCGTGGAGACAGAATTGAGTATCGTCGTTTTGCTGCCGTATTGATTTTGAAAGAAATGGCAGAAAATGCTTCCACAGTGTTCAATGTTCATGTACCTGAGTTTGTTGATGCTATCTGGGTTGCTTTGAGAGATCCAGTATTGCCTATCCGAGAGCGAGCTGTGGAAGCTCTGCGTGCTTGCCTTGGTGTTATTGAGAAACGTGAGACACGATGGCGTGTGCAATGGTATTATCGTATGTTTGAAGCTACACAGGATGGATTGGGTAAAAATGCTTCTGTTCACAGCATTCATGGTTCTTTACTTGCAGTTGGAGAGCTTTTAAGGAATACAGGTGAATTCATGATGTCAAGGTATAGGGAAGTTGCAGAAATTGTCCTTAGGTATCTTGACCACAAGGATAGGCTGGTTCGCCTGAGCATAACTTCACTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTTACAAACTACTTGGAGACATGTATGAATCATATCCTCGCTGTTTTACGACAATCAGCTGAACTACGCTCCAGTGGTTTTATTGCACTTGGGGAGATGGCCGGTGCTTTGGACGGGGAACTGTTTTTCTATTTGGGCCAAATTACACCTCACTTACGTGAAGCAATTGCTCCACGTAGAGGGCGACCCTCACTTGAGGCTTTGGCTTGTGTTGGAAATATTGCAAAAGCAATGGGGCCTGCTATGGAACATGATGTTCGTGGTCTTTTGGATGTTATGTTCGCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCCCTTGAAAAGATAACTACCAGTATACCATCTTTGCTGCCTACCATCCAAGATCGGTTGCTAGAATGCATTTCAGTGGTTCTTTCAAAATCCCAACATCCTCAGGGAAGGCCAGTTGTTGGAATGGGTAGAGGAAATTTAATAAATATCCCTCAGCAAGTTTCAGACCTCAGTGGCTCATCCCTTGTGCAGCTTGCTTTGCAGACTCTAGCTCGTTTCAATTTCAAGGGTCACGACCTTCTTGAATTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGACGGAGCTATCCGAAAAGATGCTGCCCTATGTTGTTGCAGATTGGTTGCAAATTCCTTTTCTGGTGTACAATATGCTTCTGGTAGGTCAAATCGGGGAAAGCGACGCCGTCTTGTTGAGGAGATTGTTGAAAAGCTTCTCATTGAGGCTGTTGCAGATGCTGATGTGATTGTTCGCCATTCAATATTTTCTTCTCTACATGGGAATAGAGGCTTTGACGATTTCCTGGCTCAGGCTGACAGTCTCAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGCGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAATCCTGCTTATGTTCTTCCAGCTCTTCGCCGCCATCTTATACAGTTGTTGACTTACTTGGGTCAGAGTGCAGATAGCAAATGCAGGGAAGAAAGTGCGAAGTTACTGGGTTGTTTAATACGTAACTGTGAAAGACTAATACTTCCGTACATTGCTCCTATACACAAGGCACTTGTGGCAAGACTTGTGGATGGCACTGGTGTCGGCTCGAATAATGGCATCATTAGTGGAGTTCTTGTAACTGTTGGGGATCTTGCTAGAGTGGGTGGGTTTGCAATGAGAAAATATATACGAGAGCTTATGCCTTTGATCGTTGAAGCTTTATTGGATGGAGCGGCTGTCACAAAACGGGAAGTGGCTGTAGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCGTATAATGAATATCCACTGTTACTTGGCTTACTCTTGAAGTTGCTTAACGGTGAGTTGGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTTGGAATTATGGGTGCTCTAGATCCTCATGTGCATAAACGAAATCAGCAAAGCTTGCCTGGGTCACATGGAGATGTTCCTCGTAATGCCAGTGATTCTGGTCAACACATCCAATCTGTGGATGAATTGCCTATGGACTTGTGGCCATCATTTGCAACCTCTGAAGATTATTATTCTACGGTTGCAATCAATTCTCTCATGCGAATACTTAGGGACCCTTCACTTGGTACTTACCACCTAAAGGTTGTCGGATCACTTATGTTCATATTCAAGGTTTTGCCTGACCTTTTCAATATAGTCCGCACATGTGATGATGCTTTGAAGGATTTTATAACATGGAAGCTTGGAACTCTAGTGTCTATTGTGCGTCAGCACATACGGAAATATCTGCATGAATTGTTGGTTCTAATTTCAGAACTATGGTCAACCTTTAGTTTCCCTGCTGGTCCCCGTCCTCAACCCGGCTATCCAGTTCTTCATCTGGTGGAGCAGCTTTGTTTGTCCCTGAATGATGAGTTCAGGACGTATCTTCCTGATATTCTTCCATGCTGTATCCAAGTTTTAAGTGACGCAGAACGGTGTAATGACTACACTTACGTTCTTGACATCCTCCATACACTTGAAGTATTTAGTGATAATCATTCCAATTTCCAAAATATAGGAACATTGGATGAACATATGCATCTTCTTCTTCCCGCTCTAATCCGTTTGTTCAAAGTGGATGCTTCAGTGGATATAAGACGTGCTGCTATCAAAACTTTGACCAAACTCATCCCTCGGGTGCAGGTTACTGGTCACATATCTTCTCTTGTGCATCACTTGAAGCTAGTCTTAGATGGGAAGGATGACCTCCGGAAGGACACTGTCGATGCGCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGATTTCACCATCTTTATCCCATCCATACACAAACTTTTGTTAAAACATCGCTTACGGCACAAGGGGTTTGAGGAAATTGAAGGCCATTTGCAGCGACGTGAACCTTTGGCTCCCCAAAGATTAAGTCGACGACTTCCAGAGGTTGTTGCTGACCGTTCAACTGATTTGGAGATTGACCCTTATGATGATGTGACTGATGTGCAGAAAAAACTTCGAAGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACACTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCACTACGAACCTGTGCAAGGCTTGCACAGTCGCAGCCATTTGTTGGGCGGGAGTTGTTTGCAGCTGGATTTGTTAGCTGTTGGGCACAGCTAAACGAGACTAGTCAGAAACAGTTAGTTCGGAGTTTGGAGATGGCATTTTCATCTCCAAATATTCCTCCTGAAATTTTGGCGACACTGCTTAATCTGGCAGAGTTCATGGAACATGATGAGAAACCCCTTCCTATAGACATTCGCCTACTTGGTGCTCTTGCAGAGAAGTGTCGTGCATTTGCAAAGGCCTTGCATTACAAAGAAATGGAATTTGAAGGAGCACGTTCCAAGAAGATGGATGCCAATCCTGTTGCTGTCGTTGAAGCACTTATACATATAAACAACCAGTTACAACAGCATGAGGCTGCAGTTGGAATATTGACCTATGCCCAGCAAAATTTGGATGTTCAACTGAAAGAATCATGGTATGAGAAGTTGCAGCGATGGGATGATGCTCTCAAGGCCTACACTGCAAAAGCATCTCAAGCATCGAGTCAACATCTTATTTTGGATGCTACTTTAGGTCGTATGCGATGCCTTGCTGCCTTGGCTCGATGGGAAGAGCTTAACAACCTGTTTAAGGAATACTGGACCCCAGCTGAGCCTGCTGCTCGACTGGAAATGGCCCCAATGGCTGCAAGTGCTGCTTGGAATATGGGAGAGTGGGATCAAATGGCCGAATATGTGTCTCGATTGGACGATGGTGATGAGACCAAACTTAGGGGATTGGGGAACACTGCTGCTAGTGGAGATGGAAGCAGTAGTGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGATGCAAGTATGATGAAGCACGCGAGTATGTTGAGAGAGCCAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATACCAATATGGTTCGTGTTCAGCAGCTATCAGAACTTGAGGAGGTCATCGATTATTGTACACTTCCTCTGGGGAATCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAACGTTGAGGTGTGGCAGGCACTTTTAGCAGTGAGAGCACTTGTTCTACCTCCTACTGAAGATGTTGATACTTGGCTAAAGTTTGCTACCCTCTGTCGGAAAAGTGGGCGACTTAGCCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTATGATCCCGAGACATCTCATGAAAACGTGCGATACCATGGGCCTCCACAAGTGATGCTTGCATACTTGAAATACCAATGGTCACTTGGAGAAGACGTCAAACGCAAGGAAGCGTTTGCTAGGCTGCAGAATTTGGCAATGGAGCTCTCAACTTCACCTAGCATTGAATCAGTTACGCCAATTGGCTTGATGAGCTGTAGTACTCCAAGTGTTCCACTCATTGCCCGTGTGTATCTCAAACTCGGAGAATGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCGTGTTGCCTTCCGAAATGCAACTCAATGTGCAAACAAATGGGCAAAGGCTTGGCACACATGGGCATTGTTCAATACAGCTGTTATGTCTCTTTTTACTGTCAGAGGGTATGCAAGTGCTGCTTCCCAGTTTGTTGTTGCAGCAGTCACTGGATATTTTCACTCAATCGCCTGTTCAGCAAATACTAAAGGTGTTGATGATAGCTTACAGGATATTCTTCGTCTTCTTACATTGTGGTTCAACCATGGAGCTAGTGCTGAAGTTCAAATGGCGTTACAAAAGGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGAGGTCTTGTTTCTGTTTTATCTGTGTGTTTATGTGAGTTTGCCAGTTAGAATTAGCATGTGCTCCAGAAAGGAGACAACAAAGCGTGTTGCTCTTATGTACCCTCTCCTAGTAGCCTGTAAATCAATAAGTAATTTACGCAAAGCTGCTGCTGAAGAAGTGGTTGACAAAGTTCGACAGCATAGTGGTTTACTTGTGGATCAGGCACAACTTGTTTCAACGGAACTAATCCGGGTCGCAATACTTTGGCATGAAATGTGGCATGAGGCTCTGGAAGAAGCTAGTCGTCTGTATTTTGGTGAACATAACATTGAGGGCATGCTAAAAGTATTGGAGCCATTACATGAAATGCTCGAGGAAGGGGCTATGAGGAATAATACCACAATAAAAGAGACAACATTCATTGAGGCCTATCGTCATGAATTGCTGGAGGCTTATGAATGTTGCATGAAATATAAGAGAACTGGAAAAGATGCCGAGCTTACTCAGGCATGGGATCTTTACTATCATGTTTTCAGACGAATAGATAAGCAGCTGCAAAGTCTCACTACATTGGACTTGGAGTCTGTCTCGCCAGAGTTGTTAGAATGTCGTGATTTGGAGCTGGCTGTTCCTGGTACATATCGAGCAGAATCTCCGGTGGTGACAATTAGATCATTTGCACGCCAGCTTGTTGTCATAACATCCAAACAGCGACCAAGGAAATTAACAATTCATGGGAGTGATGGTGAGGAATATGCCTTCTTGCTGAAGGGACATGAAGACTTGCGCCAAGATGAACGTGTCATGCAGCTTTTTGGTTTGGTGAACACGCTGCTGGAGAATTCTAGAAAAACTCAAGAGAAGGATCTCTCCATTCAACGATATTCTGTCATTCCTTTATCTCCAAACAGTGGACTGATTGGTTGGGTCCCAAATTGTGACACCCTTCACCATCTCATTAGGGAGTACAGGGATGCCAGAAAGCTGCTTTGCAAGTTTTACCCAATAAAAGCAGATGTACTAACAGGATTGGTTGGGTTGTATCTTCCTTCTCCAGAAGGAGAACCAAGTTTCTTCAGCCTTGGTTTTGAGAGTTTTGCTCCGGATTATGATCATTTGCCACTCATAGCTAAAGTGGAAGTATTTGAGTATGCCCTGCACAATACAGAGGGAAATGACCTTTCAAGGGTTCTTTGGCTGAAAAGTCGCACTTCAGAGGTATGGCTGGAGAGGAGGACAAACTATACGAGAAGTTTGGCAGTCATGAGCATGGTTGGTTACCTTCTTGGTCTCGGTGATCGTCACCCAAGCAATCTTATGCTGCACCGCTATAGTGGGAAGATCTTGCACATCGACTTTGGGGACTGCTTTGAAGCTTCTATGAATCGCGAAAAGTTTCCTGAGAAGGTCCCCTTTCGATTGACTAGAATGCTAGTGAAAGCAATGGAGGTTAGTGGTATAGAGGGCAACTTCCGTTCAACTTGTGAAAATGTCATGCAAGTTCTGCGAACACATAAAGATAGTGTTATGGCCATGATGGAGGCCTTTGTTCATGATCCTCTCATCAACTGGCGTCTCTTTAACTTCAATGAAGTTCCACAAGTGGCAATGCTAGCAAATAGTCATGCTCCTCCTGTTGTGGATGCCGAAGAACCGGCTCCAGCTAGAGAGCTTCCCCAACCTCAACGTGGTGCTCGTGAAAGGGAACTTCTTCAGGCTGTTAATCAACTTGGTGATGCTAATGAGGTCTTAAATGAGCGCGCTGTGGTTGTCATGGCTCGGATGAGTAATAAGCTTACTGGGCGTGATTTTTCCACCAGCTCCGCTGTATCATCTAGCTCTATTCAACATGCAGTAGACCACAGCACGCTGATTTCTGGAGATACTCGAGAAGTTGATCATGGTTTATCTGTTAAGCTGCAAGTTCAAAAGTTGATTGGCCAAGCAACTTCTTATGAAAATTTGTGCCAGAATTATGTCGGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

2477

Amino Acids

278.14

Weight (kDa)

6.36

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
mTOR_dom PF11865 759 - 915 2e-39 Serine/threonine-protein kinase mTOR domain
FAT PF02259 1427 - 1793 3.7e-95 FAT domain
FRB_dom PF08771 1897 - 1999 5.5e-38 FKBP12-rapamycin binding domain
PI3_PI4_kinase PF00454 2067 - 2341 2.4e-79 Phosphatidylinositol 3- and 4-kinase
FATC PF02260 2452 - 2477 1.2e-07 FATC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2708
AasI GACNNNNNNGTC 1 cut(s) 383
AatII GACGTC 1 cut(s) 4947
Acc36I ACCTGC 1 cut(s) 3151
AccB7I CCANNNNNTGG 4 cut(s) 1379, 2494, 3665, 6359
AccBSI CCGCTC 3 cut(s) 2210, 3074, 4678
AccI GTMKAC 4 cut(s) 1285, 2351, 3406, 7305
AccII CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
AccIII TCCGGA 2 cut(s) 3228, 6550
AclI AACGTT 1 cut(s) 4701
AcoI YGGCCR 6 cut(s) 3, 1047, 2519, 4329, 6978, 7387
AcyI GRCGYC 4 cut(s) 72, 1651, 4944, 7021
AflIII ACRYGT 4 cut(s) 104, 968, 2681, 6247
AhdI GACNNNNNGTC 1 cut(s) 6028
AhlI ACTAGT 1 cut(s) 3722
AjiI CACGTC 3 cut(s) 2360, 3119, 3377
AjnI CCWGG 7 cut(s) 387, 1770, 2434, 4493, 4815, 5116, 6079
AleI CACNNNNGTG 1 cut(s) 1104
AloI GAACNNNNNNTCC 2 cut(s) 1211, 1243
Alw21I GWGCWC 9 cut(s) 268, 2267, 2395, 3871, 3937, 4738, 4995, 5531, 7144
AlwNI CAGNNNCTG 2 cut(s) 1706, 5297
Ama87I CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
Aor13HI TCCGGA 2 cut(s) 3228, 6550
ApaI GGGCCC 1 cut(s) 4654
ArsI GACNNNNNNTTYG 2 cut(s) 3388, 3420
Asp700I GAANNNNTTC 3 cut(s) 1680, 6843, 7158
AspLEI GCGC 6 cut(s) 1796, 3250, 3549, 6237, 7209, 7211
AsuC2I CCSGG 4 cut(s) 54, 424, 2838, 5699
AsuII TTCGAA 2 cut(s) 3493, 4450
AsuNHI GCTAGC 1 cut(s) 7060
AvaI CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
AvaII GGWCC 6 cut(s) 385, 2590, 2822, 4245, 6376, 6856
AxyI CCTNAGG 3 cut(s) 23, 860, 1362
BaeGI GKGCMC 2 cut(s) 3709, 4654
BalI TGGCCA 3 cut(s) 2521, 6980, 7389
BanII GRGCYC 4 cut(s) 268, 4654, 4995, 5110
BarI GAAGNNNNNNTAC 4 cut(s) 739, 771, 3969, 4001
BauI CACGAG 1 cut(s) 7143
BbsI GAAGAC 3 cut(s) 4947, 5363, 6234
Bbv12I GWGCWC 9 cut(s) 268, 2267, 2395, 3871, 3937, 4738, 4995, 5531, 7144
BbvCI CCTCAGC 1 cut(s) 1413
BceAI ACGGC 3 cut(s) 503, 1638, 3350
BcgI CGANNNNNNTGC 2 cut(s) 2059, 2093
BciT130I CCWGG 7 cut(s) 389, 1772, 2436, 4495, 4817, 5118, 6081
BciVI GTATCC 3 cut(s) 95, 2939, 4674
BclI TGATCA 2 cut(s) 6559, 7345
BcnI CCSGG 4 cut(s) 54, 424, 2838, 5699
BcuI ACTAGT 1 cut(s) 3722
BfmI CTRYAG 7 cut(s) 438, 2235, 3846, 4020, 4973, 5044, 6778
BfuAI ACCTGC 1 cut(s) 3151
BfuI GTATCC 3 cut(s) 95, 2939, 4674
BglI GCCNNNNNGGC 1 cut(s) 4286
BglII AGATCT 2 cut(s) 4823, 6789
BlpI GCTNAGC 1 cut(s) 4254
BmcAI AGTACT 2 cut(s) 2367, 5050
Bme18I GGWCC 6 cut(s) 385, 2590, 2822, 4245, 6376, 6856
BmeRI GACNNNNNGTC 1 cut(s) 6028
BmeT110I CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
BmgBI CACGTC 3 cut(s) 2360, 3119, 3377
BmrI ACTGGG 4 cut(s) 1991, 2277, 5269, 7257
BmtI GCTAGC 1 cut(s) 7064
BmuI ACTGGG 4 cut(s) 1991, 2277, 5269, 7257
BpiI GAAGAC 3 cut(s) 4947, 5363, 6234
BplI GAGNNNNNCTC 4 cut(s) 6020, 6052, 6532, 6564
BpmI CTGGAG 8 cut(s) 1003, 5515, 5915, 6305, 6486, 6695, 7092, 7347
Bpu10I CCTNAGC 3 cut(s) 896, 1413, 1776
Bpu1102I GCTNAGC 1 cut(s) 4254
Bpu14I TTCGAA 2 cut(s) 3493, 4450
BpuEI CTTGAG 5 cut(s) 1163, 4097, 4122, 4610, 6288
BpuMI CCSGG 4 cut(s) 54, 424, 2838, 5699
Bsa29I ATCGAT 2 cut(s) 943, 4602
BsaAI YACGTR 3 cut(s) 1106, 1124, 1997
BsaBI GATNNNNATC 2 cut(s) 3957, 6558
BsaHI GRCGYC 4 cut(s) 72, 1651, 4944, 7021
BsaI GGTCTC 2 cut(s) 4358, 6743
BsaWI WCCGGW 3 cut(s) 3228, 6103, 6550
BsaXI ACNNNNNCTCC 8 cut(s) 2097, 2127, 2199, 2229, 4929, 4959, 6178, 6208
Bse118I RCCGGY 2 cut(s) 1049, 7102
Bse21I CCTNAGG 3 cut(s) 23, 860, 1362
Bse3DI GCAATG 9 cut(s) 438, 710, 1185, 2025, 2157, 4992, 5067, 6897, 7062
Bse8I GATNNNNATC 2 cut(s) 3957, 6558
BseAI TCCGGA 2 cut(s) 3228, 6550
BseBI CCWGG 7 cut(s) 389, 1772, 2436, 4495, 4817, 5118, 6081
BseCI ATCGAT 2 cut(s) 943, 4602
BseJI GATNNNNATC 2 cut(s) 3957, 6558
BseMI GCAATG 9 cut(s) 438, 710, 1185, 2025, 2157, 4992, 5067, 6897, 7062
BsePI GCGCGC 1 cut(s) 7207
BseRI GAGGAG 4 cut(s) 1679, 4607, 6694, 7068
BseSI GKGCMC 2 cut(s) 3709, 4654
BseYI CCCAGC 4 cut(s) 1856, 3530, 4044, 4249
BsgI GTGCAG 9 cut(s) 35, 85, 1469, 1965, 3179, 3500, 3606, 6590, 6755
Bsh1236I CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
BshVI ATCGAT 2 cut(s) 943, 4602
BsiHKAI GWGCWC 9 cut(s) 268, 2267, 2395, 3871, 3937, 4738, 4995, 5531, 7144
BsiHKCI CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
BsiSI CCGG 9 cut(s) 54, 423, 1050, 2838, 3229, 5698, 6104, 6551, 7103
BslFI GGGAC 8 cut(s) 4, 2603, 2808, 2863, 6234, 6362, 6824, 6842
BsmBI CGTCTC 2 cut(s) 13, 7028
BsmFI GGGAC 8 cut(s) 4, 2603, 2808, 2863, 6234, 6362, 6824, 6842
BsmI GAATGC 5 cut(s) 316, 458, 768, 1331, 6882
Bso31I GGTCTC 2 cut(s) 4358, 6743
BsoBI CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
Bsp119I TTCGAA 2 cut(s) 3493, 4450
Bsp120I GGGCCC 1 cut(s) 4650
Bsp13I TCCGGA 2 cut(s) 3228, 6550
Bsp1407I TGTACA 3 cut(s) 1612, 2257, 4610
Bsp1720I GCTNAGC 1 cut(s) 4254
Bsp19I CCATGG 2 cut(s) 4885, 5392
Bsp68I TCGCGA 2 cut(s) 1836, 6837
BspDI ATCGAT 2 cut(s) 943, 4602
BspEI TCCGGA 2 cut(s) 3228, 6550
BspFNI CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
BspHI TCATGA 5 cut(s) 822, 4867, 5884, 6711, 7000
BspMAI CTGCAG 3 cut(s) 442, 4024, 4977
BspMI ACCTGC 1 cut(s) 3151
BspOI GCTAGC 1 cut(s) 7064
BspQI GCTCTTC 2 cut(s) 1905, 4209
BspT104I TTCGAA 2 cut(s) 3493, 4450
BspTNI GGTCTC 2 cut(s) 4358, 6743
BsrBI CCGCTC 3 cut(s) 2210, 3074, 4678
BsrDI GCAATG 9 cut(s) 438, 710, 1185, 2025, 2157, 4992, 5067, 6897, 7062
BsrFI RCCGGY 2 cut(s) 1049, 7102
BsrGI TGTACA 3 cut(s) 1612, 2257, 4610
BssAI RCCGGY 2 cut(s) 1049, 7102
BssHII GCGCGC 1 cut(s) 7207
BssNAI GTATAC 1 cut(s) 1286
BssNI GRCGYC 4 cut(s) 72, 1651, 4944, 7021
BssSI CACGAG 1 cut(s) 7143
BssT1I CCWWGG 8 cut(s) 117, 664, 3271, 4200, 4885, 5392, 6164, 6529
Bst1107I GTATAC 1 cut(s) 1286
Bst2BI CACGAG 1 cut(s) 7143
Bst2UI CCWGG 7 cut(s) 389, 1772, 2436, 4495, 4817, 5118, 6081
Bst6I CTCTTC 2 cut(s) 1905, 4209
BstACI GRCGYC 4 cut(s) 72, 1651, 4944, 7021
BstAPI GCANNNNNTGC 8 cut(s) 437, 548, 1802, 2431, 3644, 4298, 5267, 6952
BstAUI TGTACA 3 cut(s) 1612, 2257, 4610
BstBAI YACGTR 3 cut(s) 1106, 1124, 1997
BstBI TTCGAA 2 cut(s) 3493, 4450
BstDSI CCRYGG 2 cut(s) 4885, 5392
BstEII GGTNACC 1 cut(s) 6725
BstENI CCTNNNNNAGG 5 cut(s) 1775, 3230, 3275, 3637, 4232
BstFNI CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
BstHHI GCGC 6 cut(s) 1796, 3250, 3549, 6237, 7209, 7211
BstNI CCWGG 7 cut(s) 389, 1772, 2436, 4495, 4817, 5118, 6081
BstNSI RCATGY 5 cut(s) 972, 2685, 5527, 5788, 7301
BstPI GGTNACC 1 cut(s) 6725
BstSFI CTRYAG 7 cut(s) 438, 2235, 3846, 4020, 4973, 5044, 6778
BstSLI GKGCMC 2 cut(s) 3709, 4654
BstSNI TACGTA 1 cut(s) 1997
BstUI CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
BstV2I GAAGAC 3 cut(s) 4947, 5363, 6234
BstX2I RGATCY 7 cut(s) 613, 2125, 2398, 4823, 5962, 6313, 6789
BstXI CCANNNNNNTGG 1 cut(s) 3949
BstYI RGATCY 7 cut(s) 613, 2125, 2398, 4823, 5962, 6313, 6789
BstZ17I GTATAC 1 cut(s) 1286
Bsu15I ATCGAT 2 cut(s) 943, 4602
Bsu36I CCTNAGG 3 cut(s) 23, 860, 1362
BsuI GTATCC 3 cut(s) 95, 2939, 4674
BsuTUI ATCGAT 2 cut(s) 943, 4602
BtgI CCRYGG 2 cut(s) 4885, 5392
BtgZI GCGATG 4 cut(s) 258, 3311, 4111, 4201
BtrI CACGTC 3 cut(s) 2360, 3119, 3377
BtsI GCAGTG 6 cut(s) 908, 3525, 3797, 4122, 4389, 4734
BtuMI TCGCGA 2 cut(s) 1836, 6837
BveI ACCTGC 1 cut(s) 3151
CaiI CAGNNNCTG 2 cut(s) 1706, 5297
CciI TCATGA 5 cut(s) 822, 4867, 5884, 6711, 7000
CfoI GCGC 6 cut(s) 1796, 3250, 3549, 6237, 7209, 7211
Cfr10I RCCGGY 2 cut(s) 1049, 7102
ClaI ATCGAT 2 cut(s) 943, 4602
CseI GACGC 5 cut(s) 80, 1659, 2732, 2954, 7010
CspCI CAANNNNNGTGG 4 cut(s) 1833, 1868, 2043, 2078
DraI TTTAAA 1 cut(s) 1812
DrdI GACNNNNNNGTC 1 cut(s) 383
DriI GACNNNNNGTC 1 cut(s) 6028
DseDI GACNNNNNNGTC 1 cut(s) 383
EaeI YGGCCR 6 cut(s) 3, 1047, 2519, 4329, 6978, 7387
Eam1104I CTCTTC 2 cut(s) 1905, 4209
Eam1105I GACNNNNNGTC 1 cut(s) 6028
EarI CTCTTC 2 cut(s) 1905, 4209
EciI GGCGGA 1 cut(s) 75
Ecl136II GAGCTC 2 cut(s) 266, 4993
Eco105I TACGTA 1 cut(s) 1997
Eco130I CCWWGG 8 cut(s) 117, 664, 3271, 4200, 4885, 5392, 6164, 6529
Eco147I AGGCCT 4 cut(s) 3902, 4118, 5876, 6992
Eco24I GRGCYC 4 cut(s) 268, 4654, 4995, 5110
Eco31I GGTCTC 2 cut(s) 4358, 6743
Eco47I GGWCC 6 cut(s) 385, 2590, 2822, 4245, 6376, 6856
Eco53kI GAGCTC 2 cut(s) 266, 4993
Eco81I CCTNAGG 3 cut(s) 23, 860, 1362
Eco88I CYCGRG 4 cut(s) 3153, 4856, 5819, 7335
Eco91I GGTNACC 1 cut(s) 6725
EcoICRI GAGCTC 2 cut(s) 266, 4993
EcoNI CCTNNNNNAGG 5 cut(s) 1775, 3230, 3275, 3637, 4232
EcoO109I RGGNCCY 6 cut(s) 1184, 1261, 2590, 4651, 6376, 6856
EcoO65I GGTNACC 1 cut(s) 6725
EcoRI GAATTC 2 cut(s) 818, 6289
EcoRII CCWGG 7 cut(s) 387, 1770, 2434, 4493, 4815, 5116, 6079
EcoT14I CCWWGG 8 cut(s) 117, 664, 3271, 4200, 4885, 5392, 6164, 6529
EcoT22I ATGCAT 3 cut(s) 316, 1331, 3060
EcoT38I GRGCYC 4 cut(s) 268, 4654, 4995, 5110
ErhI CCWWGG 8 cut(s) 117, 664, 3271, 4200, 4885, 5392, 6164, 6529
Esp3I CGTCTC 2 cut(s) 13, 7028
FaqI GGGAC 8 cut(s) 4, 2603, 2808, 2863, 6234, 6362, 6824, 6842
FauI CCCGC 2 cut(s) 3079, 3663
FauNDI CATATG 1 cut(s) 3054
FbaI TGATCA 2 cut(s) 6559, 7345
FblI GTMKAC 4 cut(s) 1285, 2351, 3406, 7305
FriOI GRGCYC 4 cut(s) 268, 4654, 4995, 5110
GlaI GCGC 6 cut(s) 1795, 3249, 3548, 6236, 7208, 7210
GsaI CCCAGC 4 cut(s) 1860, 3534, 4048, 4253
GsuI CTGGAG 8 cut(s) 1003, 5515, 5915, 6305, 6486, 6695, 7092, 7347
HapII CCGG 9 cut(s) 54, 423, 1050, 2838, 3229, 5698, 6104, 6551, 7103
HgaI GACGC 5 cut(s) 80, 1659, 2732, 2954, 7010
HhaI GCGC 6 cut(s) 1796, 3250, 3549, 6237, 7209, 7211
Hin1I GRCGYC 4 cut(s) 72, 1651, 4944, 7021
Hin6I GCGC 6 cut(s) 1794, 3248, 3547, 6235, 7207, 7209
HinP1I GCGC 6 cut(s) 1794, 3248, 3547, 6235, 7207, 7209
HincII GTYRAC 6 cut(s) 1926, 2480, 2802, 3407, 4151, 5632
HindII GTYRAC 6 cut(s) 1926, 2480, 2802, 3407, 4151, 5632
HindIII AAGCTT 7 cut(s) 64, 1679, 2193, 2428, 2717, 6822, 7241
HpaII CCGG 9 cut(s) 54, 423, 1050, 2838, 3229, 5698, 6104, 6551, 7103
Hpy99I CGWCG 6 cut(s) 74, 510, 1653, 3378, 3411, 4650
Hsp92I GRCGYC 4 cut(s) 72, 1651, 4944, 7021
HspAI GCGC 6 cut(s) 1794, 3248, 3547, 6235, 7207, 7209
KflI GGGWCCC 2 cut(s) 2590, 6376
Kpn2I TCCGGA 2 cut(s) 3228, 6550
Ksp22I TGATCA 2 cut(s) 6559, 7345
LguI GCTCTTC 2 cut(s) 1905, 4209
MbiI CCGCTC 3 cut(s) 2210, 3074, 4678
MfeI CAATTG 2 cut(s) 1113, 5028
MflI RGATCY 7 cut(s) 613, 2125, 2398, 4823, 5962, 6313, 6789
MlsI TGGCCA 3 cut(s) 2521, 6980, 7389
MluI ACGCGT 1 cut(s) 104
MluNI TGGCCA 3 cut(s) 2521, 6980, 7389
MlyI GAGTC 3 cut(s) 1459, 4156, 6038
MmeI TCCRAC 6 cut(s) 772, 1141, 1360, 2605, 4006, 4775
Mox20I TGGCCA 3 cut(s) 2521, 6980, 7389
Mph1103I ATGCAT 3 cut(s) 316, 1331, 3060
MroI TCCGGA 2 cut(s) 3228, 6550
MroXI GAANNNNTTC 3 cut(s) 1680, 6843, 7158
MscI TGGCCA 3 cut(s) 2521, 6980, 7389
MslI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6186, 6716
Msp20I TGGCCA 3 cut(s) 2521, 6980, 7389
MspA1I CMGCKG 6 cut(s) 1007, 3686, 4253, 5228, 6001, 7274
MspI CCGG 9 cut(s) 54, 423, 1050, 2838, 3229, 5698, 6104, 6551, 7103
MunI CAATTG 2 cut(s) 1113, 5028
Mva1269I GAATGC 5 cut(s) 316, 458, 768, 1331, 6882
MvaI CCWGG 7 cut(s) 389, 1772, 2436, 4495, 4817, 5118, 6081
MvnI CGCG 5 cut(s) 106, 1836, 4476, 6837, 7209
NciI CCSGG 4 cut(s) 54, 424, 2838, 5699
NcoI CCATGG 2 cut(s) 4885, 5392
NdeI CATATG 1 cut(s) 3054
NheI GCTAGC 1 cut(s) 7060
NmeAIII GCCGAG 1 cut(s) 5968
NruI TCGCGA 2 cut(s) 1836, 6837
NsiI ATGCAT 3 cut(s) 316, 1331, 3060
NspI RCATGY 5 cut(s) 972, 2685, 5527, 5788, 7301
NspV TTCGAA 2 cut(s) 3493, 4450
OliI CACNNNNGTG 1 cut(s) 1104
PaeI GCATGC 1 cut(s) 5788
PaeR7I CTCGAG 2 cut(s) 5819, 7335
PagI TCATGA 5 cut(s) 822, 4867, 5884, 6711, 7000
PauI GCGCGC 1 cut(s) 7207
PceI AGGCCT 4 cut(s) 3902, 4118, 5876, 6992
PciI ACATGT 2 cut(s) 968, 2681
PciSI GCTCTTC 2 cut(s) 1905, 4209
PcsI WCGNNNNNNNCGW 5 cut(s) 36, 688, 1129, 1645, 7141
PctI GAATGC 5 cut(s) 316, 458, 768, 1331, 6882
PdmI GAANNNNTTC 3 cut(s) 1680, 6843, 7158
PflFI GACNNNGTC 3 cut(s) 1653, 3239, 5455
PflMI CCANNNNNTGG 4 cut(s) 1379, 2494, 3665, 6359
PfoI TCCNGGA 1 cut(s) 5116
PleI GAGTC 3 cut(s) 1459, 4155, 6037
PpsI GAGTC 3 cut(s) 1459, 4155, 6037
Ppu21I YACGTR 3 cut(s) 1106, 1124, 1997
PpuMI RGGWCCY 3 cut(s) 2590, 6376, 6856
PscI ACATGT 2 cut(s) 968, 2681
PsiI TTATAA 1 cut(s) 2708
Psp124BI GAGCTC 2 cut(s) 268, 4995
Psp1406I AACGTT 1 cut(s) 4701
Psp5II RGGWCCY 3 cut(s) 2590, 6376, 6856
Psp6I CCWGG 7 cut(s) 387, 1770, 2434, 4493, 4815, 5116, 6079
PspEI GGTNACC 1 cut(s) 6725
PspFI CCCAGC 4 cut(s) 1856, 3530, 4044, 4249
PspGI CCWGG 7 cut(s) 387, 1770, 2434, 4493, 4815, 5116, 6079
PspOMI GGGCCC 1 cut(s) 4650
PspPPI RGGWCCY 3 cut(s) 2590, 6376, 6856
PspXI VCTCGAGB 1 cut(s) 5819
PsrI GAACNNNNNNTAC 2 cut(s) 2358, 2390
PstI CTGCAG 3 cut(s) 442, 4024, 4977
PstNI CAGNNNCTG 2 cut(s) 1706, 5297
PsuI RGATCY 7 cut(s) 613, 2125, 2398, 4823, 5962, 6313, 6789
PsyI GACNNNGTC 3 cut(s) 1653, 3239, 5455
PteI GCGCGC 1 cut(s) 7207
PvuII CAGCTG 5 cut(s) 1007, 3686, 4253, 5228, 6001
RruI TCGCGA 2 cut(s) 1836, 6837
RseI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6186, 6716
SacI GAGCTC 2 cut(s) 268, 4995
SalI GTCGAC 1 cut(s) 3405
SapI GCTCTTC 2 cut(s) 1905, 4209
ScaI AGTACT 2 cut(s) 2367, 5050
SchI GAGTC 3 cut(s) 1459, 4156, 6038
SfcI CTRYAG 7 cut(s) 438, 2235, 3846, 4020, 4973, 5044, 6778
Sfr274I CTCGAG 2 cut(s) 5819, 7335
SfuI TTCGAA 2 cut(s) 3493, 4450
SinI GGWCC 6 cut(s) 385, 2590, 2822, 4245, 6376, 6856
SlaI CTCGAG 2 cut(s) 5819, 7335
SmiMI CAYNNNNRTG 5 cut(s) 1104, 1334, 2448, 6186, 6716
SmlI CTYRAG 7 cut(s) 1142, 4112, 4137, 4589, 5819, 6303, 7335
SmoI CTYRAG 7 cut(s) 1142, 4112, 4137, 4589, 5819, 6303, 7335
SnaBI TACGTA 1 cut(s) 1997
SpeI ACTAGT 1 cut(s) 3722
SphI GCATGC 1 cut(s) 5788
SseBI AGGCCT 4 cut(s) 3902, 4118, 5876, 6992
SspI AATATT 4 cut(s) 1168, 1731, 3775, 4032
SstI GAGCTC 2 cut(s) 268, 4995
StuI AGGCCT 4 cut(s) 3902, 4118, 5876, 6992
StyI CCWWGG 8 cut(s) 117, 664, 3271, 4200, 4885, 5392, 6164, 6529
TatI WGTACW 7 cut(s) 1612, 2257, 2365, 4610, 5048, 6414, 6466
TauI GCSGC 5 cut(s) 50, 60, 243, 1908, 2213
TspGWI ACGGA 7 cut(s) 1563, 2010, 2274, 2770, 3071, 5702, 6911
Tth111I GACNNNGTC 3 cut(s) 1653, 3239, 5455
Van91I CCANNNNNTGG 4 cut(s) 1379, 2494, 3665, 6359
VpaK11BI GGWCC 6 cut(s) 385, 2590, 2822, 4245, 6376, 6856
XagI CCTNNNNNAGG 5 cut(s) 1775, 3230, 3275, 3637, 4232
XbaI TCTAGA 2 cut(s) 2395, 6293
XceI RCATGY 5 cut(s) 972, 2685, 5527, 5788, 7301
XcmI CCANNNNNNNNNTGG 3 cut(s) 4501, 4931, 7133
XhoI CTCGAG 2 cut(s) 5819, 7335
XmiI GTMKAC 4 cut(s) 1285, 2351, 3406, 7305
XmnI GAANNNNTTC 3 cut(s) 1680, 6843, 7158
ZraI GACGTC 1 cut(s) 4945
ZrmI AGTACT 2 cut(s) 2367, 5050
Zsp2I ATGCAT 3 cut(s) 316, 1331, 3060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.