pycom10g09700

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0005708
Physical Location & Seq
Reverse (-)
12853151 .. 12871836
18686 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g09700.33

Sequence Viewer

Length: 5046 bp
ATGTTTGAGGCTACCCAAGATGGATTGGGTAAAAATGCTTCCATTCACAGCATACATGGTTCTTTGCTTGCAGTTGGAGAACTTTTAAGGAATACAGGTGAATTTATGATGTCTAGGTACAGGGAAGTTGCCGATATTGTCCTTAGATATCTTGAGCACCGGGACAGGCTGGTTCGCCTGAGCATAACTTCGCTGCTACCTCGAATTGCTCATTTTCTGCGTGATCGATTTGTCACAAACTACTTAACGATATGTATGAATCATATTCTTGCAGTTTTGCGAATACCAGCAGAACGCTCCAGTGGTTTTATTGCCCTTGGGGAGATGGCTGGTGCTTTGGACGGGGAACTTGTCCATTATTTGCCGACAATAACGTCTCATTTACGTGATGCGATTGCTCCACGTAGAGGTAGACCCTCTCTTGAGGCTTTGGCTTGTGTTGGAAACATAGCAAAAGCAATGGGGCCAGCTATGGAACCTCATGTTCGTGGTCTTTTGGATGTTATGTTCTCTGCTGGTCTTTCCCCTACGCTTGTTGAGGCACTTGAGCAAATTACTACCAGTATTACATCTTTGCTTCCTACCATCCAAGATCGGTTACTTGACTGCATTTCGGCGGTTCTTTCAAAATCCCACCAGTCTCAGGGAAGGTCGGCTGTTGGCATGGGTAGAGGAAATTTAATGAATGTCCCTCAGCAAGCGTCAGACCTCAGTGGTTCAGCCCTTGTGCAGCTTGCTTTGCAGACTTTAGCTCGTTTTAATTTCAAGGGCCATGACCTTCTTGAGTTTGCAAGAGAATCAGTTGTTGTATATTTGGATGATGATGATGGAGCCATCCGGAAAGATGCTGCCCTATGTTGTTGCAGATTAGTTGCAAATTCCTTTTCTGGTATGCAATATGCTTCTGGTAGGGCAAATCGGGGAAAGCGACGACGTCTTGTTGAGGAGATTGTGGAAAAGCTTCTCAGTGAAGCTGTTGCGGATGCTGATGTTATTGTTCGTCATTCAATATTTTCCTCCCTTCATGGGAATAGAGGTTTTGATGATTTTTTGGCGCAGGCTGATAGTCTGAGCGCAGTTTTTGCTGCTTTAAATGATGAGGATTTTGATGTTCGAGAGTTTGCAATTTCTGTGGCTGGGAGGTTGTCAGAAAAAAACCCTGCTTATGTTCTTCCAGCACTTCGTCGGCATCTTATACAGTTGTTGACTTACCTGGGGCAGAGTGCAGATACCAAATGCCGGGAAGAAAGCGCGAAGTTACTGGGTTGTTTAATACGAAACTGTGAACGACTAATACTTCCATATATTGCTCCGATACACAAGGCACTTGTTGCAAGACTTACTGATGGCACTGGTGTGAACGCAAATAATGGCATTATTAGTGGAGTTCTTGTAACTGTTGGGGATCTGGCTAAAGTGGGTGGTTTTGCAATGCGAAAATACATTCCTGAGCTTATGCCTTTAATTGTTGATGCTTTACTGGATGGAGCAGCTGTCACCAAACGGGAAGTGGCGGTCGCTACTCTTGGTCAAGTTGTACAGAGCACTGGGTATGTCATAACTCCATACAATGAATATCCACTGTTACTTGGCTTGCTCTTGAAGTTACTGAACGGTGAATTAGCATGGTCTACAAGACGTGAAGTACTAAAGGTTCTCGGAATTATGGGTGCTCTAGACCCTCATGTGCATAAACGAAATCAACAAAGCCTGCCTGGGTCACATGGAGATGTTACCCGTAATGCGAGTGAATCTGGTCAACACTTTCAGTCTGTGGATGAATTGCCTATGGACCTGTGGCCATCATTTGCAACCTCAGAAGACTATTATTCTACGGTTGCAATCAACTCGCTCATGCGTATACTTAGGGACCCTTCACTTGCTACTTACCACCTAAAGGTGGTCGGATCTCTTATGTTCATATTCAAGTCGATGGGTCTTGGTTGTGTTCCATACTTACCAAAGGTTTTGCCTGATCTCTTACATATAGTCCGGACATGTGATGATGCTTTAAAGGATTTTATAACATGGAAGCTTGGCACACTAGTGTCTATTGTGCGTCAGCATATTCGGAAATATCTGCATGAGTTGCTCGTTCTAATTTCAGAACTATGGTCAACCTTCAGTTTTCCTGCTGCTGGCCATCCTCAACCTGGTTATCCAGTTCTGCATCTGGTGGAGCAGCTTTGCTTGGCTCTCAATGATGAATTTCGAACGTACCTTCCTGATATTCTTCCATGTTGTATTCAAGTTCTAAGTGATGCAGAACGGTACAACGATTATACTTATGTTCTGGACATCCTCCGTACCCTTGAAGTGTTTGGTGGAACATTGGATGAACATATGCATCTTCTTCTTCCTGCACTCATTCGCTTGTTTAAAGTTGATGCTTCTGTGGACATAAGACGTGCTGCTATTAAAACTTTGACGAAACTGATCCCTCGTGTGCAGGTTGCTGGTCACATATCTTCTCTCGTGCATCACTTAAAGCTAGTCTTAGATGGCAAGAACAATGAGCTCCGGAAGGATGCTGTTGATGCCCTTTGTTGTCTAGCTCATGCCCTTGGGGAGGACTTCACAATTTTTATCCCATCAATACACAAACTTCTGCTGAAACATAATTTAAGGCATAAGGAATTCGAGGAAATTGAAGGGCGGTTGAAGCGACGTGAACCTCTAATTTTGGGGAGTACAACTTTTCAAAGATTAAGCAGACGGCTTCCAGTAGAGGTTATTACTGACCGTTGGAATGATTTAGAGATTGACCCTTATGATAATGGGTCAGATATGCAGAAACAACTTCGAGGCCATCAGGTCAATGATGGTCGATTACGCACTGCTGGGGAGGCTTCTCAGCGCAGTACCAAAGAAGATTGGGCAGAATGGATGAGACATTTTAGTATTGAACTTCTGAAGGAATCTCCTTCTCCTGCCCTACGAACCTGTGCAAGGCTTGCACAGTTGCAGCCATTTGTTGGGCGGGAGCTATTTGCAGCTGGTTTTGTCAGCTGTTGGGCACAGCTGAATGAGACTAGTCAGAAACAGTTAGTTCGAAGTTTGGAGATGGCATTTTCATCCCCAAATATACCTCCTGAAATTTTGGCAACACTTCTTAATTTGGCAGAGTTCATGGAACATGATGAGAAGCCTCTTCCTATAGACATTCGCCTGCTTGGTGCTCTTGCAGAAAAGTGTCGTGCATTTGCAAAGGCATTGCATTACAAAGAAATGGAATTTGAAGGAGCGCGCTCCAAGAAGATGGATGCCAACCCTGTTGCTGTAGTTGAAGCACTTATACATATAAACAACCAGTTACACCAGCATGAGGCTGCAGTTGGAATATTGACCTTTGCCCAACAAAATATGGATGTCCAACTCAAAGAGTCATGGTATGAGAAGTTGCAGCGATGGGACGATGCTCTCAAGGCCTACACAGCAAAAGCCTCTCAAGCGTCGAGTCCACATCTTGTTTTGGATGCCACTTTAGGTCGGATGCGATGCCTTGCTGCTTTGGCACAATGGGAGGAGCTTAACAACCTGTGTAAGGAATATTGGACCCCAGCTGAACCGGCTGCTCGATTGGAAATGGCACCAATGGCTGCTAATGCTGCTTGGAACATGGGGGAGTGGGATCAAATGGCAGAATATGTGTCACGGTTAGATGATGGTGATGAAACCAAGCTTAGGGGCTTGGGGAACACTGCTGCTAGTGGTGATGGAAGCAGTAATGGCACATTCTTTAGAGCTGTTTTGTTAGTTCGAAGAGGAAAGTATGATGAAGCACGCGAGTATGTTGAAAGAGCTAGGAAATGCTTGGCAACAGAGCTTGCTGCTTTGGTTTTGGAGAGCTATGAACGTGCATATAACAATATGGTCCGTGTTCAGCAGCTGTCAGAACTTGAGGAGGTAATTGATTATTGCACACTTCCTCTGGTAAACCCTGTTGCTGAAGGACGACGGGCCCTTATTCGCAATATGTGGAATGAGCGGATACAAGGAGCAAAACGTAATGTTGAGGTATGGCAGGTACTTTTAGCAGTTAGAGCACTTGTTCTACCTCCGACAGAAGATGTTGACACTTGGCTTAAGTTTGCTTCCCTTTGTCGGAAAAGTGGGCGAATTAGTCAGGCTAGATCTACTTTAGTCAAACTCTTACAGTATGATCCTGAAGATGTTACTCATGAAAGCCTGCGGTACGATGGGCCTCCACAAGTGATGCTTGCATATTTGAAATACCAATGGTCGCTTGGAGAGGATGTTAAGCGCAAGGAAGCATTTGCAAGGCTACAGAATTTGGCAATAGAGCTTTCAAGTCCACCCAGTATTCAACCAGTTACACCAACTGGCTTGATGAGCTCTAGTAGTCCAAGTGTTCCACTTATCGCCCGTGTGTATCTCAAATTGGGAGCCTGGAATTGGGCTCTTTCTCCTGGATTGGATGATGATTCTATACAAGAAATTCTCAATGCCTTCCGCACTGCAACTCAATGTGCCAATAAATGGGCCAAGGCATGGCACACGTGGGCACTGTTTAATACAGCAGTGATGTCACTTTATACCGTAAGAGGTTATGCAAGTGTTGCGTCACAGTTTGTTGTTGCAGCGGTCACTGGATATTTTCACTCAATAGCATGTTCAGCGAATACCAAAGGTGTTGATGATAGTTTACAGGATATTCTTCGTCTTCTTACGTTGTGGTTCAACCATGGAGCTACTGCAGAAGTGCAAATGGCACTACAGAAAGGGTTTGCACATGTTAATATTAACACATGGCTGGTGGTTTTGCCTCAAATAATCGCCAGGATACATTCTAATAACTATGCTGTGAGGGAACTGATACAGTCGCTTTTGGTGCGAATTGGACAAAGTCATCCACAGGCTCTTATGTACCCTCTTCTTGTAGCATGTAAATCAATAAGTAATTTACGTAGAGCTGCAGCTCAAGAAGTGGTTGACAAAGTTCGACAGCATAGTGGTGTGCTTGTGGATCGATCAGGCACAGCTTGTGTCAAAGGAACTAATCCGGGTTGCAATACTTTGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0001067 GO:0001558 GO:0002682 GO:0002683 GO:0002697 GO:0002698 GO:0002831 GO:0002832 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005844 GO:0006139 GO:0006259 GO:0006281 GO:0006351 GO:0006355 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006661 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008610 GO:0008654 GO:0009058 GO:0009059 GO:0009303 GO:0009605 GO:0009606 GO:0009607 GO:0009615 GO:0009628 GO:0009629 GO:0009630 GO:0009719 GO:0009725 GO:0009733 GO:0009743 GO:0009744 GO:0009745 GO:0009756 GO:0009790 GO:0009791 GO:0009793 GO:0009889 GO:0009891 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010115 GO:0010116 GO:0010154 GO:0010182 GO:0010243 GO:0010467 GO:0010468 GO:0010506 GO:0010507 GO:0010556 GO:0010557 GO:0010565 GO:0010604 GO:0010628 GO:0010646 GO:0010647 GO:0010928 GO:0010929 GO:0014070 GO:0016070 GO:0016072 GO:0016301 GO:0016303 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018130 GO:0019216 GO:0019219 GO:0019222 GO:0019438 GO:0019538 GO:0019637 GO:0019747 GO:0022414 GO:0023051 GO:0023052 GO:0023056 GO:0030258 GO:0030307 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031328 GO:0031329 GO:0031330 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0033554 GO:0034285 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0035004 GO:0036092 GO:0036211 GO:0040008 GO:0040019 GO:0042221 GO:0042493 GO:0042802 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043455 GO:0043621 GO:0043900 GO:0043901 GO:0044087 GO:0044089 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044260 GO:0044267 GO:0044271 GO:0044424 GO:0044464 GO:0045017 GO:0045472 GO:0045828 GO:0045834 GO:0045893 GO:0045927 GO:0045935 GO:0045995 GO:0046474 GO:0046483 GO:0046486 GO:0046488 GO:0046677 GO:0046834 GO:0046854 GO:0046889 GO:0046890 GO:0048316 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048583 GO:0048584 GO:0048585 GO:0048608 GO:0048731 GO:0048856 GO:0050687 GO:0050688 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051173 GO:0051239 GO:0051240 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0052742 GO:0060255 GO:0061458 GO:0062012 GO:0062013 GO:0065007 GO:0065008 GO:0070887 GO:0071310 GO:0071322 GO:0071324 GO:0071329 GO:0071704 GO:0080090 GO:0080134 GO:0090069 GO:0090070 GO:0090304 GO:0090407 GO:0097159 GO:0097305 GO:0097327 GO:0097659 GO:0098781 GO:0140096 GO:1900457 GO:1900459 GO:1901355 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901576 GO:1901654 GO:1901698 GO:1901700 GO:1901701 GO:1902659 GO:1902661 GO:1902680 GO:1902930 GO:1902932 GO:1903506 GO:1903508 GO:1990904 GO:2000026 GO:2000112 GO:2000232 GO:2000234 GO:2001141
KEGG Pathways
Metabolic & Signaling
ko01521 ko01522 ko04012 ko04066 ko04072 ko04136 ko04138 ko04139 ko04140 ko04150 ko04151 ko04152 ko04211 ko04212 ko04213 ko04218 ko04371 ko04630 ko04659 ko04714 ko04910 ko04919 ko04920 ko04930 ko04931 ko05165 ko05167 ko05200 ko05205 ko05206 ko05210 ko05212 ko05214 ko05215 ko05221 ko05224 ko05225 ko05226 ko05230 ko05231 map01521 map01522 map04012 map04066 map04072 map04136 map04138 map04139 map04140 map04150 map04151 map04152 map04211 map04212 map04213 map04218 map04371 map04630 map04659 map04714 map04910 map04919 map04920 map04930 map04931 map05165 map05167 map05200 map05205 map05206 map05210 map05212 map05214 map05215 map05221 map05224 map05225 map05226 map05230 map05231
Pfam Domains
Protein Families
DUF3385 FAT FATC FRB_dom PI3_PI4_kinase

Protein Analysis

1682

Amino Acids

187.18

Weight (kDa)

7.07

Isoelectric Point (pI)

42.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2024
AasI GACNNNNNNGTC 1 cut(s) 373
AatII GACGTC 1 cut(s) 937
Acc36I ACCTGC 2 cut(s) 2440, 4015
AccB1I GGYRCC 1 cut(s) 3589
AccB7I CCANNNNNTGG 3 cut(s) 2975, 4500, 4512
AccBSI CCGCTC 1 cut(s) 3988
AccI GTMKAC 3 cut(s) 412, 1631, 1861
AccII CGCG 3 cut(s) 1253, 3247, 3786
AccIII TCCGGA 3 cut(s) 837, 1992, 2520
AciI CCGC 9 cut(s) 617, 980, 1514, 2656, 2980, 3988, 4192, 4474, 4604
AclWI GGATC 6 cut(s) 1413, 1915, 2431, 3639, 4157, 4995
AcoI YGGCCR 2 cut(s) 1799, 2140
AcsI RAATTY 9 cut(s) 101, 676, 877, 2207, 2636, 3096, 3233, 4291, 4458
AcuI CTGAAG 4 cut(s) 2107, 2933, 3969, 4188
AcvI CACGTG 1 cut(s) 4521
AcyI GRCGYC 1 cut(s) 934
AflII CTTAAG 1 cut(s) 4085
AflIII ACRYGT 3 cut(s) 1997, 4518, 4753
AhlI ACTAGT 2 cut(s) 2044, 3032
AjiI CACGTC 3 cut(s) 1640, 2408, 2669
AjnI CCWGG 6 cut(s) 1212, 1714, 2152, 4409, 4429, 4799
AleI CACNNNNGTG 2 cut(s) 1355, 2045
AloI GAACNNNNNNTCC 4 cut(s) 491, 523, 2206, 2238
Alw21I GWGCWC 7 cut(s) 159, 1547, 1675, 2520, 3181, 4048, 4358
Alw26I GTCTC 4 cut(s) 381, 645, 2884, 3023
AlwI GGATC 6 cut(s) 1413, 1915, 2431, 3639, 4157, 4995
AlwNI CAGNNNCTG 1 cut(s) 3889
Aor13HI TCCGGA 3 cut(s) 837, 1992, 2520
AoxI GGCC 9 cut(s) 464, 769, 1799, 2140, 2806, 3426, 3960, 4202, 4503
ApaI GGGCCC 1 cut(s) 3964
ApoI RAATTY 9 cut(s) 101, 676, 877, 2207, 2636, 3096, 3233, 4291, 4458
Asp700I GAANNNNTTC 1 cut(s) 960
AspLEI GCGC 7 cut(s) 1057, 1076, 1253, 2859, 3247, 3249, 4266
AsuC2I CCSGG 3 cut(s) 161, 1241, 5025
AsuHPI GGTGA 5 cut(s) 110, 1489, 1628, 3680, 3725
AsuII TTCGAA 3 cut(s) 2212, 3052, 3760
AvaII GGWCC 4 cut(s) 1792, 1870, 3555, 3874
BaeGI GKGCMC 3 cut(s) 3019, 3964, 4528
BalI TGGCCA 2 cut(s) 1801, 2142
BanI GGYRCC 1 cut(s) 3589
BanII GRGCYC 4 cut(s) 2520, 3964, 4358, 4423
BarI GAAGNNNNNNTAC 4 cut(s) 22, 54, 3279, 3311
BauI CACGAG 2 cut(s) 2442, 2474
BbrPI CACGTG 1 cut(s) 4521
BbsI GAAGAC 2 cut(s) 1827, 4676
Bbv12I GWGCWC 7 cut(s) 159, 1547, 1675, 2520, 3181, 4048, 4358
BbvCI CCTCAGC 1 cut(s) 693
BceAI ACGGC 1 cut(s) 2732
BcgI CGANNNNNNTGC 2 cut(s) 1830, 1864
BciT130I CCWGG 6 cut(s) 1214, 1716, 2154, 4411, 4431, 4801
BciVI GTATCC 2 cut(s) 3984, 4797
BcnI CCSGG 3 cut(s) 161, 1241, 5025
BcoDI GTCTC 4 cut(s) 381, 645, 2884, 3023
BcuI ACTAGT 2 cut(s) 2044, 3032
BfmI CTRYAG 7 cut(s) 3156, 3279, 3330, 4286, 4716, 4736, 4935
BfrI CTTAAG 1 cut(s) 4085
BfuAI ACCTGC 2 cut(s) 2440, 4015
BfuI GTATCC 2 cut(s) 3984, 4797
BglII AGATCT 1 cut(s) 4133
BmcAI AGTACT 1 cut(s) 1647
Bme1390I CCNGG 9 cut(s) 161, 1214, 1241, 1716, 2154, 4411, 4431, 4801, 5025
Bme18I GGWCC 4 cut(s) 1792, 1870, 3555, 3874
BmgBI CACGTC 3 cut(s) 1640, 2408, 2669
BmiI GGNNCC 9 cut(s) 465, 477, 832, 1871, 1872, 3557, 3591, 3962, 4408
BmrFI CCNGG 9 cut(s) 161, 1214, 1241, 1716, 2154, 4411, 4431, 4801, 5025
BmrI ACTGGG 3 cut(s) 1271, 1557, 4314
BmuI ACTGGG 3 cut(s) 1271, 1557, 4314
BpiI GAAGAC 2 cut(s) 1827, 4676
BpmI CTGGAG 1 cut(s) 283
Bpu10I CCTNAGC 4 cut(s) 179, 693, 1449, 3683
Bpu14I TTCGAA 3 cut(s) 2212, 3052, 3760
BpuEI CTTGAG 8 cut(s) 173, 443, 566, 803, 3407, 3432, 3920, 4926
BpuMI CCSGG 3 cut(s) 161, 1241, 5025
Bsa29I ATCGAT 2 cut(s) 226, 4990
BsaAI YACGTR 4 cut(s) 386, 404, 4521, 4928
BsaHI GRCGYC 1 cut(s) 934
BsaJI CCNNGG 6 cut(s) 316, 1213, 1715, 2563, 4506, 4705
BsaWI WCCGGW 3 cut(s) 837, 1992, 2520
BsaXI ACNNNNNCTCC 6 cut(s) 1377, 1407, 1479, 1509, 4242, 4272
Bse118I RCCGGY 1 cut(s) 3568
Bse3DI GCAATG 3 cut(s) 465, 1437, 3212
BseAI TCCGGA 3 cut(s) 837, 1992, 2520
BseBI CCWGG 6 cut(s) 1214, 1716, 2154, 4411, 4431, 4801
BseCI ATCGAT 2 cut(s) 226, 4990
BseDI CCNNGG 6 cut(s) 316, 1213, 1715, 2563, 4506, 4705
BseMI GCAATG 3 cut(s) 465, 1437, 3212
BseMII CTCAG 9 cut(s) 170, 656, 707, 724, 979, 1061, 1440, 1830, 2867
BsePI GCGCGC 1 cut(s) 3245
BseRI GAGGAG 3 cut(s) 959, 3539, 3917
BseSI GKGCMC 3 cut(s) 3019, 3964, 4528
BseYI CCCAGC 3 cut(s) 1136, 2840, 3559
BsgI GTGCAG 4 cut(s) 749, 1245, 2346, 2468
Bsh1236I CGCG 3 cut(s) 1253, 3247, 3786
Bsh1285I CGRYCG 1 cut(s) 1518
BshFI GGCC 9 cut(s) 466, 771, 1801, 2142, 2808, 3428, 3962, 4204, 4505
BshNI GGYRCC 1 cut(s) 3589
BshVI ATCGAT 2 cut(s) 226, 4990
BsiEI CGRYCG 1 cut(s) 1518
BsiHKAI GWGCWC 7 cut(s) 159, 1547, 1675, 2520, 3181, 4048, 4358
BsiSI CCGG 7 cut(s) 160, 838, 1240, 1993, 2521, 3569, 5024
BslFI GGGAC 4 cut(s) 176, 674, 1883, 3425
BsmAI GTCTC 4 cut(s) 381, 645, 2884, 3023
BsmBI CGTCTC 1 cut(s) 381
BsmFI GGGAC 4 cut(s) 176, 674, 1883, 3425
BsnI GGCC 9 cut(s) 466, 771, 1801, 2142, 2808, 3428, 3962, 4204, 4505
Bsp119I TTCGAA 3 cut(s) 2212, 3052, 3760
Bsp120I GGGCCC 1 cut(s) 3960
Bsp13I TCCGGA 3 cut(s) 837, 1992, 2520
Bsp1407I TGTACA 1 cut(s) 1537
Bsp19I CCATGG 1 cut(s) 4705
BspACI CCGC 9 cut(s) 617, 980, 1514, 2656, 2980, 3988, 4192, 4474, 4604
BspANI GGCC 9 cut(s) 466, 771, 1801, 2142, 2808, 3428, 3962, 4204, 4505
BspCNI CTCAG 9 cut(s) 171, 655, 706, 723, 978, 1062, 1441, 1829, 2866
BspDI ATCGAT 2 cut(s) 226, 4990
BspEI TCCGGA 3 cut(s) 837, 1992, 2520
BspFNI CGCG 3 cut(s) 1253, 3247, 3786
BspHI TCATGA 1 cut(s) 4180
BspLI GGNNCC 9 cut(s) 465, 477, 832, 1871, 1872, 3557, 3591, 3962, 4408
BspMAI CTGCAG 3 cut(s) 3334, 4720, 4939
BspMI ACCTGC 2 cut(s) 2440, 4015
BspPI GGATC 6 cut(s) 1413, 1915, 2431, 3639, 4157, 4995
BspT104I TTCGAA 3 cut(s) 2212, 3052, 3760
BspT107I GGYRCC 1 cut(s) 3589
BspTI CTTAAG 1 cut(s) 4085
BsrBI CCGCTC 1 cut(s) 3988
BsrDI GCAATG 3 cut(s) 465, 1437, 3212
BsrFI RCCGGY 1 cut(s) 3568
BsrGI TGTACA 1 cut(s) 1537
BssAI RCCGGY 1 cut(s) 3568
BssECI CCNNGG 6 cut(s) 316, 1213, 1715, 2563, 4506, 4705
BssHII GCGCGC 1 cut(s) 3245
BssNAI GTATAC 1 cut(s) 1862
BssNI GRCGYC 1 cut(s) 934
BssSI CACGAG 2 cut(s) 2442, 2474
BssT1I CCWWGG 4 cut(s) 316, 2563, 4506, 4705
Bst1107I GTATAC 1 cut(s) 1862
Bst2BI CACGAG 2 cut(s) 2442, 2474
Bst2UI CCWGG 6 cut(s) 1214, 1716, 2154, 4411, 4431, 4801
Bst6I CTCTTC 3 cut(s) 3156, 3757, 4899
BstACI GRCGYC 1 cut(s) 934
BstAFI CTTAAG 1 cut(s) 4085
BstAPI GCANNNNNTGC 5 cut(s) 1082, 1331, 2089, 2954, 4580
BstAUI TGTACA 1 cut(s) 1537
BstBAI YACGTR 4 cut(s) 386, 404, 4521, 4928
BstBI TTCGAA 3 cut(s) 2212, 3052, 3760
BstDSI CCRYGG 1 cut(s) 4705
BstENI CCTNNNNNAGG 3 cut(s) 2567, 2947, 3542
BstFNI CGCG 3 cut(s) 1253, 3247, 3786
BstHHI GCGC 7 cut(s) 1057, 1076, 1253, 2859, 3247, 3249, 4266
BstMAI GTCTC 4 cut(s) 381, 645, 2884, 3023
BstMCI CGRYCG 1 cut(s) 1518
BstNI CCWGG 6 cut(s) 1214, 1716, 2154, 4411, 4431, 4801
BstNSI RCATGY 4 cut(s) 2001, 4635, 4757, 4908
BstSCI CCNGG 9 cut(s) 159, 1212, 1239, 1714, 2152, 4409, 4429, 4799, 5023
BstSFI CTRYAG 7 cut(s) 3156, 3279, 3330, 4286, 4716, 4736, 4935
BstSLI GKGCMC 3 cut(s) 3019, 3964, 4528
BstSNI TACGTA 1 cut(s) 4928
BstUI CGCG 3 cut(s) 1253, 3247, 3786
BstV2I GAAGAC 2 cut(s) 1827, 4676
BstX2I RGATCY 3 cut(s) 1405, 1907, 4133
BstXI CCANNNNNNTGG 1 cut(s) 3259
BstYI RGATCY 3 cut(s) 1405, 1907, 4133
BstZ17I GTATAC 1 cut(s) 1862
Bsu15I ATCGAT 2 cut(s) 226, 4990
BsuI GTATCC 2 cut(s) 3984, 4797
BsuRI GGCC 9 cut(s) 466, 771, 1801, 2142, 2808, 3428, 3962, 4204, 4505
BsuTUI ATCGAT 2 cut(s) 226, 4990
BtgI CCRYGG 1 cut(s) 4705
BtgZI GCGATG 2 cut(s) 3421, 3511
BtrI CACGTC 3 cut(s) 1640, 2408, 2669
BtsI GCAGTG 4 cut(s) 2835, 3699, 4476, 4548
BveI ACCTGC 2 cut(s) 2440, 4015
CaiI CAGNNNCTG 1 cut(s) 3889
CciI TCATGA 1 cut(s) 4180
CfoI GCGC 7 cut(s) 1057, 1076, 1253, 2859, 3247, 3249, 4266
Cfr10I RCCGGY 1 cut(s) 3568
ClaI ATCGAT 2 cut(s) 226, 4990
CseI GACGC 4 cut(s) 690, 2048, 3441, 4572
CsiI ACCWGGT 1 cut(s) 2152
CspCI CAANNNNNGTGG 2 cut(s) 1113, 1148
DraI TTTAAA 3 cut(s) 1092, 2013, 2380
DrdI GACNNNNNNGTC 1 cut(s) 373
DseDI GACNNNNNNGTC 1 cut(s) 373
EaeI YGGCCR 2 cut(s) 1799, 2140
Eam1104I CTCTTC 3 cut(s) 3156, 3757, 4899
EarI CTCTTC 3 cut(s) 3156, 3757, 4899
Ecl136II GAGCTC 2 cut(s) 2518, 4356
Eco105I TACGTA 1 cut(s) 4928
Eco130I CCWWGG 4 cut(s) 316, 2563, 4506, 4705
Eco147I AGGCCT 1 cut(s) 3428
Eco24I GRGCYC 4 cut(s) 2520, 3964, 4358, 4423
Eco32I GATATC 1 cut(s) 149
Eco47I GGWCC 4 cut(s) 1792, 1870, 3555, 3874
Eco53kI GAGCTC 2 cut(s) 2518, 4356
Eco57I CTGAAG 4 cut(s) 2107, 2933, 3969, 4188
Eco72I CACGTG 1 cut(s) 4521
EcoICRI GAGCTC 2 cut(s) 2518, 4356
EcoNI CCTNNNNNAGG 3 cut(s) 2567, 2947, 3542
EcoO109I RGGNCCY 2 cut(s) 1870, 3961
EcoRI GAATTC 1 cut(s) 2636
EcoRII CCWGG 6 cut(s) 1212, 1714, 2152, 4409, 4429, 4799
EcoRV GATATC 1 cut(s) 149
EcoT14I CCWWGG 4 cut(s) 316, 2563, 4506, 4705
EcoT22I ATGCAT 1 cut(s) 2349
EcoT38I GRGCYC 4 cut(s) 2520, 3964, 4358, 4423
ErhI CCWWGG 4 cut(s) 316, 2563, 4506, 4705
Esp3I CGTCTC 1 cut(s) 381
FalI AAGNNNNNCTT 4 cut(s) 2480, 2512, 4203, 4235
FaqI GGGAC 4 cut(s) 176, 674, 1883, 3425
FauI CCCGC 1 cut(s) 2973
FauNDI CATATG 1 cut(s) 2343
FblI GTMKAC 3 cut(s) 412, 1631, 1861
FriOI GRGCYC 4 cut(s) 2520, 3964, 4358, 4423
GlaI GCGC 7 cut(s) 1056, 1075, 1252, 2858, 3246, 3248, 4265
GsaI CCCAGC 3 cut(s) 1140, 2844, 3563
GsuI CTGGAG 1 cut(s) 283
HaeIII GGCC 9 cut(s) 466, 771, 1801, 2142, 2808, 3428, 3962, 4204, 4505
HapII CCGG 7 cut(s) 160, 838, 1240, 1993, 2521, 3569, 5024
HgaI GACGC 4 cut(s) 690, 2048, 3441, 4572
HhaI GCGC 7 cut(s) 1057, 1076, 1253, 2859, 3247, 3249, 4266
Hin1I GRCGYC 1 cut(s) 934
Hin6I GCGC 7 cut(s) 1055, 1074, 1251, 2857, 3245, 3247, 4264
HinP1I GCGC 7 cut(s) 1055, 1074, 1251, 2857, 3245, 3247, 4264
HincII GTYRAC 5 cut(s) 1206, 1760, 2118, 4075, 4954
HindII GTYRAC 5 cut(s) 1206, 1760, 2118, 4075, 4954
HindIII AAGCTT 3 cut(s) 959, 2033, 3680
HinfI GANTC 7 cut(s) 259, 797, 1751, 2918, 3383, 3457, 4445
HpaII CCGG 7 cut(s) 160, 838, 1240, 1993, 2521, 3569, 5024
HphI GGTGA 5 cut(s) 110, 1489, 1628, 3680, 3725
Hpy99I CGWCG 6 cut(s) 933, 936, 1188, 2670, 3457, 3960
Hsp92I GRCGYC 1 cut(s) 934
HspAI GCGC 7 cut(s) 1055, 1074, 1251, 2857, 3245, 3247, 4264
KflI GGGWCCC 1 cut(s) 1870
Kpn2I TCCGGA 3 cut(s) 837, 1992, 2520
MabI ACCWGGT 1 cut(s) 2152
MbiI CCGCTC 1 cut(s) 3988
MflI RGATCY 3 cut(s) 1405, 1907, 4133
MlsI TGGCCA 2 cut(s) 1801, 2142
MluNI TGGCCA 2 cut(s) 1801, 2142
MlyI GAGTC 2 cut(s) 3392, 3466
MmeI TCCRAC 9 cut(s) 55, 421, 1885, 2726, 3316, 3397, 3470, 4085, 4085
Mox20I TGGCCA 2 cut(s) 1801, 2142
Mph1103I ATGCAT 1 cut(s) 2349
MroI TCCGGA 3 cut(s) 837, 1992, 2520
MroXI GAANNNNTTC 1 cut(s) 960
MscI TGGCCA 2 cut(s) 1801, 2142
MslI CAYNNNNRTG 7 cut(s) 384, 486, 1355, 1728, 2045, 3321, 4974
Msp20I TGGCCA 2 cut(s) 1801, 2142
MspA1I CMGCKG 7 cut(s) 1493, 2996, 3009, 3022, 3563, 3889, 4604
MspCI CTTAAG 1 cut(s) 4085
MspI CCGG 7 cut(s) 160, 838, 1240, 1993, 2521, 3569, 5024
MspR9I CCNGG 9 cut(s) 161, 1214, 1241, 1716, 2154, 4411, 4431, 4801, 5025
MvaI CCWGG 6 cut(s) 1214, 1716, 2154, 4411, 4431, 4801
MvnI CGCG 3 cut(s) 1253, 3247, 3786
NciI CCSGG 3 cut(s) 161, 1241, 5025
NcoI CCATGG 1 cut(s) 4705
NdeI CATATG 1 cut(s) 2343
NlaIV GGNNCC 9 cut(s) 465, 477, 832, 1871, 1872, 3557, 3591, 3962, 4408
NmuCI GTSAC 8 cut(s) 232, 1495, 1719, 2459, 3651, 4548, 4584, 4606
NsiI ATGCAT 1 cut(s) 2349
NspI RCATGY 4 cut(s) 2001, 4635, 4757, 4908
NspV TTCGAA 3 cut(s) 2212, 3052, 3760
OliI CACNNNNGTG 2 cut(s) 1355, 2045
PagI TCATGA 1 cut(s) 4180
PauI GCGCGC 1 cut(s) 3245
PceI AGGCCT 1 cut(s) 3428
PciI ACATGT 2 cut(s) 1997, 4753
PcsI WCGNNNNNNNCGW 1 cut(s) 925
PdmI GAANNNNTTC 1 cut(s) 960
PfeI GAWTC 5 cut(s) 259, 797, 1751, 2918, 4445
PflFI GACNNNGTC 2 cut(s) 933, 4866
PflMI CCANNNNNTGG 3 cut(s) 2975, 4500, 4512
PfoI TCCNGGA 1 cut(s) 4429
PleI GAGTC 2 cut(s) 3391, 3465
PmaCI CACGTG 1 cut(s) 4521
PmlI CACGTG 1 cut(s) 4521
PpsI GAGTC 2 cut(s) 3391, 3465
Ppu21I YACGTR 4 cut(s) 386, 404, 4521, 4928
PpuMI RGGWCCY 1 cut(s) 1870
PscI ACATGT 2 cut(s) 1997, 4753
PsiI TTATAA 1 cut(s) 2024
Psp124BI GAGCTC 2 cut(s) 2520, 4358
Psp5II RGGWCCY 1 cut(s) 1870
Psp6I CCWGG 6 cut(s) 1212, 1714, 2152, 4409, 4429, 4799
PspCI CACGTG 1 cut(s) 4521
PspFI CCCAGC 3 cut(s) 1136, 2840, 3559
PspGI CCWGG 6 cut(s) 1212, 1714, 2152, 4409, 4429, 4799
PspN4I GGNNCC 9 cut(s) 465, 477, 832, 1871, 1872, 3557, 3591, 3962, 4408
PspOMI GGGCCC 1 cut(s) 3960
PspPPI RGGWCCY 1 cut(s) 1870
PsrI GAACNNNNNNTAC 4 cut(s) 1278, 1310, 1638, 1670
PstI CTGCAG 3 cut(s) 3334, 4720, 4939
PstNI CAGNNNCTG 1 cut(s) 3889
PsuI RGATCY 3 cut(s) 1405, 1907, 4133
PsyI GACNNNGTC 2 cut(s) 933, 4866
PteI GCGCGC 1 cut(s) 3245
PvuII CAGCTG 6 cut(s) 1493, 2996, 3009, 3022, 3563, 3889
RseI CAYNNNNRTG 7 cut(s) 384, 486, 1355, 1728, 2045, 3321, 4974
SacI GAGCTC 2 cut(s) 2520, 4358
ScaI AGTACT 1 cut(s) 1647
SchI GAGTC 2 cut(s) 3392, 3466
ScrFI CCNGG 9 cut(s) 161, 1214, 1241, 1716, 2154, 4411, 4431, 4801, 5025
SexAI ACCWGGT 1 cut(s) 2152
SfcI CTRYAG 7 cut(s) 3156, 3279, 3330, 4286, 4716, 4736, 4935
SfuI TTCGAA 3 cut(s) 2212, 3052, 3760
SinI GGWCC 4 cut(s) 1792, 1870, 3555, 3874
SmiMI CAYNNNNRTG 7 cut(s) 384, 486, 1355, 1728, 2045, 3321, 4974
SmlI CTYRAG 9 cut(s) 152, 422, 545, 782, 3422, 3447, 3899, 4085, 4941
SmoI CTYRAG 9 cut(s) 152, 422, 545, 782, 3422, 3447, 3899, 4085, 4941
SnaBI TACGTA 1 cut(s) 4928
SpeI ACTAGT 2 cut(s) 2044, 3032
SseBI AGGCCT 1 cut(s) 3428
SsiI CCGC 9 cut(s) 617, 980, 1514, 2656, 2980, 3988, 4192, 4474, 4604
SspI AATATT 4 cut(s) 1011, 3342, 3551, 4762
SstI GAGCTC 2 cut(s) 2520, 4358
StuI AGGCCT 1 cut(s) 3428
StyD4I CCNGG 9 cut(s) 159, 1212, 1239, 1714, 2152, 4409, 4429, 4799, 5023
StyI CCWWGG 4 cut(s) 316, 2563, 4506, 4705
TatI WGTACW 3 cut(s) 1537, 1645, 2690
TfiI GAWTC 5 cut(s) 259, 797, 1751, 2918, 4445
TseFI GTSAC 8 cut(s) 232, 1495, 1719, 2459, 3651, 4548, 4584, 4606
Tsp45I GTSAC 8 cut(s) 232, 1495, 1719, 2459, 3651, 4548, 4584, 4606
TspGWI ACGGA 2 cut(s) 2294, 3866
Tth111I GACNNNGTC 2 cut(s) 933, 4866
Van91I CCANNNNNTGG 3 cut(s) 2975, 4500, 4512
Vha464I CTTAAG 1 cut(s) 4085
VpaK11BI GGWCC 4 cut(s) 1792, 1870, 3555, 3874
XagI CCTNNNNNAGG 3 cut(s) 2567, 2947, 3542
XapI RAATTY 9 cut(s) 101, 676, 877, 2207, 2636, 3096, 3233, 4291, 4458
XbaI TCTAGA 1 cut(s) 1675
XceI RCATGY 4 cut(s) 2001, 4635, 4757, 4908
XcmI CCANNNNNNNNNTGG 3 cut(s) 1507, 2150, 4244
XmiI GTMKAC 3 cut(s) 412, 1631, 1861
XmnI GAANNNNTTC 1 cut(s) 960
ZraI GACGTC 1 cut(s) 935
ZrmI AGTACT 1 cut(s) 1647
Zsp2I ATGCAT 1 cut(s) 2349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.