MD00G1035800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008623
Physical Location & Seq
Forward (+)
6355537 .. 6358563
3027 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1035800.v1.1.491

Sequence Viewer

Length: 933 bp
ATGAAGATCTTCAACTGGGTTCATAAGCGGCTTCATCAGAAGGTTGAGTTTGCTGGAAATGTGAAAAAAACTGAACTGGAAGTGGAAACCAATCACAAGGGCACACAAGCATTTCTCAAGCAAGTCGGACTTGTTAATGTTGATGGGCTTGACGGTTGGAGGGAGGGCATTTTAACGATAGGTACCTTGGGTTTCGATCCCGTAAAACCCTTCAACCCTCAGAACGAATATTTCGTTTTGGAGAGCGCAGAGCACGATCAGGAAAACAATGGATTTTCACACTGTGGTAATGACTACGACGAAGATGATGAACATTATGATGATAATTTTGGAGATGAAGAACTGAATCCTTTAATGTTTAAAACATTTGGACACAGTTTTGAGGACGTTGGGTCAAACTCTGATGCCACTGAGAAACTAGTTGATGATGATCACGCGATCTTGACCGTTGATGGTGTTCCTCTTACACCATACGAAGGGTCCAACAATGAAATCAGTATTGCACCTCCTGATCTGTACCGTTCCGAGATCACTGATGATGATCAAAGGAAGAAGAGAGGCCAAAGAATAACCCTAGCTGACCTATTCCAGGCTGATCATGTTCATGATGTTGGTCAACTGAAGCTTGACTCCTGCAAAGTCCAGCCAAAAATGCAGAAAAAGGTGAACGTGAGAACAAAGACCGGCCTATCATTTGCCAAGAAGCTCATTCTTCGCGTCAAAGAGGATTCAAGTCCAATAAAAAATCTGCAACGACTGATGAGGAGGATGTTGAAGAGGAAGATCCATCCAGCTGAGCATGATGTCAAGGTTGACAAATCAGATGGCCAGAAGCAGCCCGGTGCAGTAGAACTCGTCACCAAGGTTAAAAGTGATGCCTCTGAATCAGTTTCTTTGCTTCAAATTCAAGGTGCTACTGCTTGTGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

311

Amino Acids

34.97

Weight (kDa)

5.64

Isoelectric Point (pI)

32.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 182
AccII CGCG 2 cut(s) 437, 717
AciI CCGC 1 cut(s) 28
AclWI GGATC 2 cut(s) 191, 778
AcoI YGGCCR 1 cut(s) 826
AcsI RAATTY 1 cut(s) 903
AcuI CTGAAG 1 cut(s) 641
AdeI CACNNNGTG 1 cut(s) 284
AfaI GTAC 2 cut(s) 184, 518
AfiI CCNNNNNNNGG 2 cut(s) 476, 589
AgsI TTSAA 6 cut(s) 13, 214, 732, 775, 902, 908
AhdI GACNNNNNGTC 1 cut(s) 391
AhlI ACTAGT 1 cut(s) 418
AjnI CCWGG 1 cut(s) 588
AluBI AGCT 4 cut(s) 578, 625, 706, 794
AluI AGCT 4 cut(s) 578, 625, 706, 794
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 2 cut(s) 191, 778
AoxI GGCC 3 cut(s) 559, 685, 826
ApeKI GCWGC 1 cut(s) 835
ApoI RAATTY 1 cut(s) 903
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 182
AspLEI GCGC 1 cut(s) 248
AspS9I GGNCC 1 cut(s) 480
AsuC2I CCSGG 1 cut(s) 840
AsuHPI GGTGA 2 cut(s) 676, 850
AvaII GGWCC 1 cut(s) 480
BaeGI GKGCMC 1 cut(s) 104
BalI TGGCCA 1 cut(s) 828
BanI GGYRCC 1 cut(s) 182
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 1 cut(s) 847
BccI CCATC 4 cut(s) 137, 446, 795, 818
BciT130I CCWGG 1 cut(s) 590
BclI TGATCA 3 cut(s) 430, 541, 595
BcnI CCSGG 1 cut(s) 840
BcuI ACTAGT 1 cut(s) 418
BfaI CTAG 2 cut(s) 419, 575
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 29, 836
BlpI GCTNAGC 1 cut(s) 795
BlsI GCNGC 2 cut(s) 30, 837
Bme1390I CCNGG 2 cut(s) 590, 840
Bme18I GGWCC 1 cut(s) 480
BmeRI GACNNNNNGTC 1 cut(s) 391
BmgT120I GGNCC 1 cut(s) 480
BmiI GGNNCC 2 cut(s) 184, 481
BmrFI CCNGG 2 cut(s) 590, 840
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 2 cut(s) 394, 865
BmuI ACTGGG 1 cut(s) 25
Bpu1102I GCTNAGC 1 cut(s) 795
BpuEI CTTGAG 1 cut(s) 101
BpuMI CCSGG 1 cut(s) 840
BsaBI GATNNNNATC 2 cut(s) 429, 540
BsaJI CCNNGG 2 cut(s) 186, 861
Bsc4I CCNNNNNNNGG 2 cut(s) 476, 589
Bse118I RCCGGY 1 cut(s) 683
Bse1I ACTGG 2 cut(s) 20, 81
Bse8I GATNNNNATC 2 cut(s) 429, 540
BseBI CCWGG 1 cut(s) 590
BseDI CCNNGG 2 cut(s) 186, 861
BseGI GGATG 2 cut(s) 774, 787
BseJI GATNNNNATC 2 cut(s) 429, 540
BseLI CCNNNNNNNGG 2 cut(s) 476, 589
BseMII CTCAG 3 cut(s) 233, 402, 786
BseNI ACTGG 2 cut(s) 20, 81
BseRI GAGGAG 1 cut(s) 778
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 1 cut(s) 847
BsgI GTGCAG 1 cut(s) 864
Bsh1236I CGCG 2 cut(s) 437, 717
BshFI GGCC 3 cut(s) 561, 687, 828
BshNI GGYRCC 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 255
BsiSI CCGG 2 cut(s) 684, 840
BslI CCNNNNNNNGG 2 cut(s) 476, 589
BsnI GGCC 3 cut(s) 561, 687, 828
Bsp1286I GDGCHC 2 cut(s) 104, 255
Bsp1720I GCTNAGC 1 cut(s) 795
BspACI CCGC 1 cut(s) 28
BspANI GGCC 3 cut(s) 561, 687, 828
BspCNI CTCAG 3 cut(s) 232, 403, 787
BspFNI CGCG 2 cut(s) 437, 717
BspHI TCATGA 1 cut(s) 604
BspLI GGNNCC 2 cut(s) 184, 481
BspPI GGATC 2 cut(s) 191, 778
BspT107I GGYRCC 1 cut(s) 182
BsrFI RCCGGY 1 cut(s) 683
BsrI ACTGG 2 cut(s) 20, 81
BssAI RCCGGY 1 cut(s) 683
BssECI CCNNGG 2 cut(s) 186, 861
BssT1I CCWWGG 2 cut(s) 186, 861
Bst2UI CCWGG 1 cut(s) 590
Bst4CI ACNGT 5 cut(s) 155, 284, 377, 448, 521
Bst6I CTCTTC 2 cut(s) 548, 770
BstDEI CTNAG 3 cut(s) 219, 411, 795
BstENI CCTNNNNNAGG 1 cut(s) 587
BstF5I GGATG 2 cut(s) 774, 787
BstFNI CGCG 2 cut(s) 437, 717
BstHHI GCGC 1 cut(s) 248
BstMWI GCNNNNNNNGC 1 cut(s) 652
BstNI CCWGG 1 cut(s) 590
BstSCI CCNGG 2 cut(s) 588, 838
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 2 cut(s) 437, 717
BstV1I GCAGC 1 cut(s) 847
BstX2I RGATCY 2 cut(s) 6, 783
BstYI RGATCY 2 cut(s) 6, 783
BsuRI GGCC 3 cut(s) 561, 687, 828
BtsCI GGATG 2 cut(s) 774, 787
BtsIMutI CAGTG 3 cut(s) 280, 408, 531
CciI TCATGA 1 cut(s) 604
CfoI GCGC 1 cut(s) 248
Cfr10I RCCGGY 1 cut(s) 683
Cfr13I GGNCC 1 cut(s) 480
CseI GACGC 1 cut(s) 706
Csp6I GTAC 2 cut(s) 183, 517
CviAII CATG 3 cut(s) 599, 605, 800
CviQI GTAC 2 cut(s) 183, 517
DdeI CTNAG 3 cut(s) 219, 411, 795
DraI TTTAAA 1 cut(s) 361
DraIII CACNNNGTG 1 cut(s) 284
DriI GACNNNNNGTC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 826
Eam1104I CTCTTC 2 cut(s) 548, 770
Eam1105I GACNNNNNGTC 1 cut(s) 391
EarI CTCTTC 2 cut(s) 548, 770
Eco130I CCWWGG 2 cut(s) 186, 861
Eco47I GGWCC 1 cut(s) 480
Eco57I CTGAAG 1 cut(s) 641
EcoNI CCTNNNNNAGG 1 cut(s) 587
EcoRII CCWGG 1 cut(s) 588
EcoT14I CCWWGG 2 cut(s) 186, 861
ErhI CCWWGG 2 cut(s) 186, 861
FaeI CATG 3 cut(s) 602, 608, 803
FaiI YATR 6 cut(s) 24, 318, 472, 600, 606, 801
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FatI CATG 3 cut(s) 598, 604, 799
FbaI TGATCA 3 cut(s) 430, 541, 595
Fnu4HI GCNGC 2 cut(s) 29, 836
FokI GGATG 2 cut(s) 774, 781
Fsp4HI GCNGC 2 cut(s) 29, 836
FspBI CTAG 2 cut(s) 419, 575
GlaI GCGC 1 cut(s) 247
GluI GCNGC 2 cut(s) 29, 836
HaeIII GGCC 3 cut(s) 561, 687, 828
HapII CCGG 2 cut(s) 684, 840
HgaI GACGC 1 cut(s) 706
HhaI GCGC 1 cut(s) 248
Hin1II CATG 3 cut(s) 602, 608, 803
Hin6I GCGC 1 cut(s) 246
HinP1I GCGC 1 cut(s) 246
HincII GTYRAC 2 cut(s) 617, 814
HindII GTYRAC 2 cut(s) 617, 814
HindIII AAGCTT 1 cut(s) 623
HinfI GANTC 4 cut(s) 346, 629, 728, 884
HpaII CCGG 2 cut(s) 684, 840
HphI GGTGA 2 cut(s) 676, 850
Hpy166II GTNNAC 3 cut(s) 617, 667, 814
Hpy188I TCNGA 7 cut(s) 39, 128, 222, 403, 526, 823, 883
Hpy188III TCNNGA 4 cut(s) 260, 442, 509, 605
Hpy8I GTNNAC 3 cut(s) 617, 667, 814
Hpy99I CGWCG 1 cut(s) 302
HpyAV CCTTC 3 cut(s) 34, 220, 470
HpyCH4III ACNGT 5 cut(s) 155, 284, 377, 448, 521
HpyCH4IV ACGT 2 cut(s) 387, 669
HpyCH4V TGCA 5 cut(s) 503, 636, 655, 751, 845
HpyF10VI GCNNNNNNNGC 1 cut(s) 652
HpyF3I CTNAG 3 cut(s) 219, 411, 795
HpySE526I ACGT 2 cut(s) 387, 669
Hsp92II CATG 3 cut(s) 602, 608, 803
HspAI GCGC 1 cut(s) 246
KpnI GGTACC 1 cut(s) 186
Ksp22I TGATCA 3 cut(s) 430, 541, 595
Lsp1109I GCAGC 1 cut(s) 847
LweI GCATC 2 cut(s) 394, 865
MaeI CTAG 2 cut(s) 419, 575
MaeII ACGT 2 cut(s) 387, 669
MaeIII GTNAC 1 cut(s) 856
MboII GAAGA 8 cut(s) 16, 314, 350, 562, 565, 704, 787, 793
MflI RGATCY 2 cut(s) 6, 783
MhlI GDGCHC 2 cut(s) 104, 255
MlsI TGGCCA 1 cut(s) 828
MluCI AATT 2 cut(s) 325, 903
MluNI TGGCCA 1 cut(s) 828
MlyI GAGTC 1 cut(s) 623
MmeI TCCRAC 3 cut(s) 106, 137, 507
Mox20I TGGCCA 1 cut(s) 828
MroXI GAANNNNTTC 1 cut(s) 8
MscI TGGCCA 1 cut(s) 828
MseI TTAA 5 cut(s) 135, 173, 353, 360, 867
MslI CAYNNNNRTG 2 cut(s) 318, 603
Msp20I TGGCCA 1 cut(s) 828
MspA1I CMGCKG 1 cut(s) 794
MspI CCGG 2 cut(s) 684, 840
MspR9I CCNGG 2 cut(s) 590, 840
MvaI CCWGG 1 cut(s) 590
MvnI CGCG 2 cut(s) 437, 717
MwoI GCNNNNNNNGC 1 cut(s) 652
NciI CCSGG 1 cut(s) 840
NlaIII CATG 3 cut(s) 602, 608, 803
NlaIV GGNNCC 2 cut(s) 184, 481
NmuCI GTSAC 1 cut(s) 856
PagI TCATGA 1 cut(s) 604
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 346, 728, 884
PkrI GCNGC 2 cut(s) 30, 837
PleI GAGTC 1 cut(s) 623
PpsI GAGTC 1 cut(s) 623
Psp6I CCWGG 1 cut(s) 588
PspGI CCWGG 1 cut(s) 588
PspN4I GGNNCC 2 cut(s) 184, 481
PspPI GGNCC 1 cut(s) 480
PsuI RGATCY 2 cut(s) 6, 783
PvuII CAGCTG 1 cut(s) 794
RsaI GTAC 2 cut(s) 184, 518
RsaNI GTAC 2 cut(s) 183, 517
RseI CAYNNNNRTG 2 cut(s) 318, 603
SaqAI TTAA 5 cut(s) 135, 173, 353, 360, 867
SatI GCNGC 2 cut(s) 29, 836
Sau96I GGNCC 1 cut(s) 480
SchI GAGTC 1 cut(s) 623
ScrFI CCNGG 2 cut(s) 590, 840
SduI GDGCHC 2 cut(s) 104, 255
SfaNI GCATC 2 cut(s) 394, 865
SinI GGWCC 1 cut(s) 480
SmiMI CAYNNNNRTG 2 cut(s) 318, 603
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
SpeI ACTAGT 1 cut(s) 418
Sse9I AATT 2 cut(s) 325, 903
SsiI CCGC 1 cut(s) 28
SspI AATATT 1 cut(s) 230
SspMI CTAG 2 cut(s) 419, 575
StyD4I CCNGG 2 cut(s) 588, 838
StyI CCWWGG 2 cut(s) 186, 861
TaaI ACNGT 5 cut(s) 155, 284, 377, 448, 521
TaiI ACGT 2 cut(s) 390, 672
TaqI TCGA 1 cut(s) 195
TasI AATT 2 cut(s) 325, 903
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 3 cut(s) 346, 728, 884
Tru1I TTAA 5 cut(s) 135, 173, 353, 360, 867
Tru9I TTAA 5 cut(s) 135, 173, 353, 360, 867
TscAI CASTG 3 cut(s) 287, 415, 538
TseFI GTSAC 1 cut(s) 856
TseI GCWGC 1 cut(s) 835
Tsp45I GTSAC 1 cut(s) 856
TspDTI ATGAA 7 cut(s) 11, 17, 23, 324, 351, 504, 593
TspRI CASTG 3 cut(s) 287, 415, 538
VpaK11BI GGWCC 1 cut(s) 480
XagI CCTNNNNNAGG 1 cut(s) 587
XapI RAATTY 1 cut(s) 903
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 419, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.