MD07G1155200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008623
Physical Location & Seq
Forward (+)
22539738 .. 22543404
3667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1155200.v1.1.491

Sequence Viewer

Length: 927 bp
ATGAAGATCTTCAACTGGGTTCATAAGCGGCTTCATCACAAAGATGGATTTTCTGCAAGTGTGAAAAAGACTGAACTGGAAATGGAAACCAATGACAAGGGCACACAAGCATTTCTCAAGCAAGTCGGACTTGTTGATGTTGACGGGCTTGACGGCTGGAGGGAGGGCATTTTAACGATAGGTACCTTGGGTTTCAATCCTCTAAAATCCTTCAACCAGAACGAATATTTTGTTTTGGAGAGCGAAGAGGACGATCAGGAAAACAATGGATTTTCACACAGTGATAATGACGACGACAATGATGAACATTATGATGATAATTTTGGAGATGAAGAACTGAATCCTTTAATGTTTAAAACATTTGGACACAGTTTTGAGGACGTTGGGTCAAACTCTGATGCCATTGAGAAACCAGCTGATGATGATCATGCGATCTTGACCGTTGGTGGTGTTCCTCTTGCACCATTTGAAGGGTCCCACTATGAAATCAGTATTGCGTCACCTGATCTGTACTGTTCCGAGATCACCGATGAAGATCAAAGGAAGAAGAGAGGTCAAAGAATAACCCTAGCTGACCTATTCCAGGCTGATCATGTTCATGATGTGGGTCAACTGAATCTTGACTCCTGCAAAGTCCAGCCAGAAATGCAGAAAAAGGTGAATGTGGGAACAAAGAGTGGCCTAGCATTTGCCAAGAAGCTCATTCCTCGCGTCAAAGAGGATTCAAGTCCAATAAAAAATCTGCAACGACTGATGAGGAGGATGTTGAAGAGGAAGATCCATCCAGCTGAGCTTGATGTCAATGTTCACAAATCAGATGGCCTGAAGCAGCCCCGTGCAGTAGAACTCATCACCAATGTTGAAAGTGATGCCTCTGACTCAGTTTCTTTGCTTCCAATTCAAGGTGCTACTGCTTGTGTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

309

Amino Acids

34.5

Weight (kDa)

5.0

Isoelectric Point (pI)

36.14

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 182
AccB1I GGYRCC 1 cut(s) 182
AccII CGCG 1 cut(s) 711
AciI CCGC 1 cut(s) 28
AclWI GGATC 1 cut(s) 772
AcuI CTGAAG 1 cut(s) 845
AdeI CACNNNGTG 1 cut(s) 281
AfaI GTAC 2 cut(s) 184, 512
AfiI CCNNNNNNNGG 3 cut(s) 470, 583, 902
AgsI TTSAA 8 cut(s) 13, 196, 214, 470, 726, 769, 863, 902
AhdI GACNNNNNGTC 1 cut(s) 385
AjnI CCWGG 1 cut(s) 582
AluBI AGCT 5 cut(s) 416, 572, 700, 788, 793
AluI AGCT 5 cut(s) 416, 572, 700, 788, 793
AlwI GGATC 1 cut(s) 772
AoxI GGCC 2 cut(s) 679, 820
ApeKI GCWGC 1 cut(s) 829
Asp700I GAANNNNTTC 1 cut(s) 8
Asp718I GGTACC 1 cut(s) 182
AspS9I GGNCC 1 cut(s) 474
AsuHPI GGTGA 4 cut(s) 492, 517, 670, 844
AvaII GGWCC 1 cut(s) 474
BaeGI GKGCMC 1 cut(s) 104
BanI GGYRCC 1 cut(s) 182
BbvI GCAGC 1 cut(s) 841
BccI CCATC 3 cut(s) 38, 789, 812
BceAI ACGGC 1 cut(s) 169
BciT130I CCWGG 1 cut(s) 584
BclI TGATCA 2 cut(s) 424, 589
BfaI CTAG 2 cut(s) 569, 683
BglII AGATCT 1 cut(s) 6
BisI GCNGC 2 cut(s) 29, 830
BlpI GCTNAGC 1 cut(s) 789
BlsI GCNGC 2 cut(s) 30, 831
Bme1390I CCNGG 1 cut(s) 584
Bme18I GGWCC 1 cut(s) 474
BmeRI GACNNNNNGTC 1 cut(s) 385
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 3 cut(s) 184, 475, 476
BmrFI CCNGG 1 cut(s) 584
BmrI ACTGGG 1 cut(s) 25
BmsI GCATC 2 cut(s) 388, 859
BmuI ACTGGG 1 cut(s) 25
BpmI CTGGAG 1 cut(s) 178
Bpu1102I GCTNAGC 1 cut(s) 789
BpuEI CTTGAG 1 cut(s) 101
BsaBI GATNNNNATC 2 cut(s) 423, 534
BsaJI CCNNGG 1 cut(s) 186
Bsc4I CCNNNNNNNGG 3 cut(s) 470, 583, 902
Bse1I ACTGG 2 cut(s) 20, 81
Bse8I GATNNNNATC 2 cut(s) 423, 534
BseBI CCWGG 1 cut(s) 584
BseDI CCNNGG 1 cut(s) 186
BseGI GGATG 2 cut(s) 768, 781
BseJI GATNNNNATC 2 cut(s) 423, 534
BseLI CCNNNNNNNGG 3 cut(s) 470, 583, 902
BseMII CTCAG 2 cut(s) 780, 894
BseNI ACTGG 2 cut(s) 20, 81
BseRI GAGGAG 1 cut(s) 772
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 1 cut(s) 841
BsgI GTGCAG 1 cut(s) 858
Bsh1236I CGCG 1 cut(s) 711
BshFI GGCC 2 cut(s) 681, 822
BshNI GGYRCC 1 cut(s) 182
BslFI GGGAC 1 cut(s) 460
BslI CCNNNNNNNGG 3 cut(s) 470, 583, 902
BsmFI GGGAC 1 cut(s) 460
BsnI GGCC 2 cut(s) 681, 822
Bsp1286I GDGCHC 1 cut(s) 104
Bsp143I GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
Bsp1720I GCTNAGC 1 cut(s) 789
BspACI CCGC 1 cut(s) 28
BspANI GGCC 2 cut(s) 681, 822
BspCNI CTCAG 2 cut(s) 781, 893
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 1 cut(s) 598
BspLI GGNNCC 3 cut(s) 184, 475, 476
BspPI GGATC 1 cut(s) 772
BspT107I GGYRCC 1 cut(s) 182
BsrI ACTGG 2 cut(s) 20, 81
BssECI CCNNGG 1 cut(s) 186
BssMI GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
BssT1I CCWWGG 1 cut(s) 186
Bst2UI CCWGG 1 cut(s) 584
Bst4CI ACNGT 4 cut(s) 281, 371, 442, 515
Bst6I CTCTTC 3 cut(s) 240, 542, 764
BstDEI CTNAG 2 cut(s) 789, 880
BstENI CCTNNNNNAGG 1 cut(s) 581
BstF5I GGATG 2 cut(s) 768, 781
BstFNI CGCG 1 cut(s) 711
BstKTI GATC 9 cut(s) 9, 256, 427, 435, 508, 525, 538, 592, 780
BstMBI GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
BstMWI GCNNNNNNNGC 1 cut(s) 646
BstNI CCWGG 1 cut(s) 584
BstSCI CCNGG 1 cut(s) 582
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 1 cut(s) 711
BstV1I GCAGC 1 cut(s) 841
BstX2I RGATCY 2 cut(s) 6, 777
BstYI RGATCY 2 cut(s) 6, 777
BsuRI GGCC 2 cut(s) 681, 822
BtsCI GGATG 2 cut(s) 768, 781
BtsIMutI CAGTG 1 cut(s) 286
CciI TCATGA 1 cut(s) 598
Cfr13I GGNCC 1 cut(s) 474
CseI GACGC 2 cut(s) 486, 700
Csp6I GTAC 2 cut(s) 183, 511
CviAII CATG 3 cut(s) 428, 593, 599
CviQI GTAC 2 cut(s) 183, 511
DdeI CTNAG 2 cut(s) 789, 880
DpnI GATC 9 cut(s) 8, 255, 426, 434, 507, 524, 537, 591, 779
DpnII GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
DraI TTTAAA 1 cut(s) 355
DraIII CACNNNGTG 1 cut(s) 281
DriI GACNNNNNGTC 1 cut(s) 385
Eam1104I CTCTTC 3 cut(s) 240, 542, 764
Eam1105I GACNNNNNGTC 1 cut(s) 385
EarI CTCTTC 3 cut(s) 240, 542, 764
Eco130I CCWWGG 1 cut(s) 186
Eco47I GGWCC 1 cut(s) 474
Eco57I CTGAAG 1 cut(s) 845
EcoNI CCTNNNNNAGG 1 cut(s) 581
EcoO109I RGGNCCY 1 cut(s) 474
EcoRII CCWGG 1 cut(s) 582
EcoT14I CCWWGG 1 cut(s) 186
ErhI CCWWGG 1 cut(s) 186
FaeI CATG 3 cut(s) 431, 596, 602
FaiI YATR 6 cut(s) 24, 312, 429, 483, 594, 600
FalI AAGNNNNNCTT 2 cut(s) 114, 146
FaqI GGGAC 1 cut(s) 460
FatI CATG 3 cut(s) 427, 592, 598
FbaI TGATCA 2 cut(s) 424, 589
Fnu4HI GCNGC 2 cut(s) 29, 830
FokI GGATG 2 cut(s) 768, 775
Fsp4HI GCNGC 2 cut(s) 29, 830
FspBI CTAG 2 cut(s) 569, 683
GluI GCNGC 2 cut(s) 29, 830
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 2 cut(s) 681, 822
HgaI GACGC 2 cut(s) 486, 700
Hin1II CATG 3 cut(s) 431, 596, 602
HincII GTYRAC 2 cut(s) 142, 611
HindII GTYRAC 2 cut(s) 142, 611
HinfI GANTC 5 cut(s) 340, 616, 623, 722, 878
HphI GGTGA 4 cut(s) 492, 517, 670, 844
Hpy166II GTNNAC 3 cut(s) 142, 611, 808
Hpy188I TCNGA 5 cut(s) 128, 397, 520, 817, 877
Hpy188III TCNNGA 4 cut(s) 257, 436, 599, 620
Hpy8I GTNNAC 3 cut(s) 142, 611, 808
Hpy99I CGWCG 1 cut(s) 296
HpyAV CCTTC 2 cut(s) 220, 464
HpyCH4III ACNGT 4 cut(s) 281, 371, 442, 515
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 6 cut(s) 56, 461, 630, 649, 745, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 646
HpyF3I CTNAG 2 cut(s) 789, 880
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 3 cut(s) 431, 596, 602
KflI GGGWCCC 1 cut(s) 474
KpnI GGTACC 1 cut(s) 186
Ksp22I TGATCA 2 cut(s) 424, 589
Kzo9I GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
Lsp1109I GCAGC 1 cut(s) 841
LweI GCATC 2 cut(s) 388, 859
MaeI CTAG 2 cut(s) 569, 683
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 1 cut(s) 498
MalI GATC 9 cut(s) 8, 255, 426, 434, 507, 524, 537, 591, 779
MboI GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
MboII GAAGA 8 cut(s) 16, 257, 344, 545, 556, 559, 781, 787
MflI RGATCY 2 cut(s) 6, 777
MhlI GDGCHC 1 cut(s) 104
MluCI AATT 2 cut(s) 319, 897
MlyI GAGTC 2 cut(s) 617, 872
MmeI TCCRAC 1 cut(s) 106
MroXI GAANNNNTTC 1 cut(s) 8
MseI TTAA 3 cut(s) 173, 347, 354
MslI CAYNNNNRTG 3 cut(s) 42, 312, 597
MspA1I CMGCKG 2 cut(s) 416, 788
MspR9I CCNGG 1 cut(s) 584
MvaI CCWGG 1 cut(s) 584
MvnI CGCG 1 cut(s) 711
MwoI GCNNNNNNNGC 1 cut(s) 646
NdeII GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
NlaIII CATG 3 cut(s) 431, 596, 602
NlaIV GGNNCC 3 cut(s) 184, 475, 476
NmuCI GTSAC 1 cut(s) 498
PagI TCATGA 1 cut(s) 598
PdmI GAANNNNTTC 1 cut(s) 8
PfeI GAWTC 3 cut(s) 340, 616, 722
PkrI GCNGC 2 cut(s) 30, 831
PleI GAGTC 2 cut(s) 617, 872
PpsI GAGTC 2 cut(s) 617, 872
PpuMI RGGWCCY 1 cut(s) 474
Psp5II RGGWCCY 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 582
PspGI CCWGG 1 cut(s) 582
PspN4I GGNNCC 3 cut(s) 184, 475, 476
PspPI GGNCC 1 cut(s) 474
PspPPI RGGWCCY 1 cut(s) 474
PsuI RGATCY 2 cut(s) 6, 777
PvuII CAGCTG 2 cut(s) 416, 788
RsaI GTAC 2 cut(s) 184, 512
RsaNI GTAC 2 cut(s) 183, 511
RseI CAYNNNNRTG 3 cut(s) 42, 312, 597
SaqAI TTAA 3 cut(s) 173, 347, 354
SatI GCNGC 2 cut(s) 29, 830
Sau3AI GATC 9 cut(s) 6, 253, 424, 432, 505, 522, 535, 589, 777
Sau96I GGNCC 1 cut(s) 474
SchI GAGTC 2 cut(s) 617, 872
ScrFI CCNGG 1 cut(s) 584
SduI GDGCHC 1 cut(s) 104
SfaNI GCATC 2 cut(s) 388, 859
SinI GGWCC 1 cut(s) 474
SmiMI CAYNNNNRTG 3 cut(s) 42, 312, 597
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 2 cut(s) 319, 897
SsiI CCGC 1 cut(s) 28
SspI AATATT 1 cut(s) 227
SspMI CTAG 2 cut(s) 569, 683
StyD4I CCNGG 1 cut(s) 582
StyI CCWWGG 1 cut(s) 186
TaaI ACNGT 4 cut(s) 281, 371, 442, 515
TaiI ACGT 1 cut(s) 384
TasI AATT 2 cut(s) 319, 897
TatI WGTACW 1 cut(s) 510
TauI GCSGC 1 cut(s) 31
TfiI GAWTC 3 cut(s) 340, 616, 722
Tru1I TTAA 3 cut(s) 173, 347, 354
Tru9I TTAA 3 cut(s) 173, 347, 354
TscAI CASTG 1 cut(s) 286
TseFI GTSAC 1 cut(s) 498
TseI GCWGC 1 cut(s) 829
Tsp45I GTSAC 1 cut(s) 498
TspDTI ATGAA 8 cut(s) 11, 17, 23, 318, 345, 498, 546, 587
TspRI CASTG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 474
XagI CCTNNNNNAGG 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 8
XspI CTAG 2 cut(s) 569, 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.