MD00G1055400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0006619
Physical Location & Seq
Reverse (-)
10389356 .. 10391336
1981 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1055400.v1.1.491

Sequence Viewer

Length: 339 bp
ATGTCAAGGCGTGAAAGAGAGAAATTGCTTTTGCTAAATACCAAGTTGTGCTTCGCAGATGTGCTGTTGAAGGTTGGCGTGTTCTTCCTGGTTCAGGCCTTGGTATATCTCATCCTTTCCAACTCATCCAACATCTTCTCCAAAACCACCAAGAGGTCCCACAGCTTCAAACCAGCTCGCTCTGTCAGTATTCGCCGGATGCTCGCTGCTCTTTCTGACTTACCCGCTGGCGGCGAGCTCTCTCCTTCAACAAACTCAACGGACACGAACACGATGATGATGCAGCCCCCTAACAGCAAAAGCAGTAGCCAAGAATACGCAACCACTCGATTCTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

113

Amino Acids

12.46

Weight (kDa)

10.28

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 225, 231
AfiI CCNNNNNNNGG 3 cut(s) 94, 153, 230
AgsI TTSAA 3 cut(s) 70, 169, 249
AjnI CCWGG 1 cut(s) 87
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
AluBI AGCT 3 cut(s) 165, 176, 238
AluI AGCT 3 cut(s) 165, 176, 238
Alw21I GWGCWC 1 cut(s) 240
AoxI GGCC 1 cut(s) 96
ApeKI GCWGC 2 cut(s) 206, 283
AspS9I GGNCC 1 cut(s) 156
AvaII GGWCC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 240
BbvI GCAGC 2 cut(s) 193, 295
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BciT130I CCWGG 1 cut(s) 89
BisI GCNGC 3 cut(s) 207, 232, 284
BlsI GCNGC 3 cut(s) 208, 233, 285
Bme1390I CCNGG 1 cut(s) 89
Bme18I GGWCC 1 cut(s) 156
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 1 cut(s) 158
BmrFI CCNGG 1 cut(s) 89
BmsI GCATC 2 cut(s) 189, 270
BsaJI CCNNGG 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bsc4I CCNNNNNNNGG 3 cut(s) 94, 153, 230
BseBI CCWGG 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 99
BseGI GGATG 3 cut(s) 111, 125, 204
BseLI CCNNNNNNNGG 3 cut(s) 94, 153, 230
BseXI GCAGC 2 cut(s) 193, 295
BshFI GGCC 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 240
BsiSI CCGG 1 cut(s) 196
BslFI GGGAC 1 cut(s) 142
BslI CCNNNNNNNGG 3 cut(s) 94, 153, 230
BsmFI GGGAC 1 cut(s) 142
BsnI GGCC 1 cut(s) 98
Bsp1286I GDGCHC 1 cut(s) 240
BspACI CCGC 2 cut(s) 225, 231
BspANI GGCC 1 cut(s) 98
BspLI GGNNCC 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 99
BssT1I CCWWGG 1 cut(s) 99
Bst2UI CCWGG 1 cut(s) 89
BstC8I GCNNGC 4 cut(s) 178, 204, 229, 236
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 3 cut(s) 111, 125, 204
BstNI CCWGG 1 cut(s) 89
BstSCI CCNGG 1 cut(s) 87
BstV1I GCAGC 2 cut(s) 193, 295
BsuRI GGCC 1 cut(s) 98
BtsCI GGATG 3 cut(s) 111, 125, 204
Cac8I GCNNGC 4 cut(s) 178, 204, 229, 236
Cfr13I GGNCC 1 cut(s) 156
CviJI RGCY 6 cut(s) 98, 165, 176, 238, 286, 309
CviKI_1 RGCY 6 cut(s) 98, 165, 176, 238, 286, 309
Ecl136II GAGCTC 1 cut(s) 238
Eco130I CCWWGG 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 98
Eco24I GRGCYC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 156
Eco53kI GAGCTC 1 cut(s) 238
EcoICRI GAGCTC 1 cut(s) 238
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoO109I RGGNCCY 1 cut(s) 156
EcoRII CCWGG 1 cut(s) 87
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 240
ErhI CCWWGG 1 cut(s) 99
FaiI YATR 1 cut(s) 106
FalI AAGNNNNNCTT 2 cut(s) 35, 67
FaqI GGGAC 1 cut(s) 142
FauI CCCGC 1 cut(s) 232
Fnu4HI GCNGC 3 cut(s) 207, 232, 284
FokI GGATG 3 cut(s) 98, 112, 211
FriOI GRGCYC 1 cut(s) 240
Fsp4HI GCNGC 3 cut(s) 207, 232, 284
GluI GCNGC 3 cut(s) 207, 232, 284
HaeIII GGCC 1 cut(s) 98
HapII CCGG 1 cut(s) 196
HinfI GANTC 1 cut(s) 330
HpaII CCGG 1 cut(s) 196
Hpy188I TCNGA 1 cut(s) 217
HpyAV CCTTC 2 cut(s) 64, 255
HpyCH4V TGCA 1 cut(s) 283
LpnPI CCDG 6 cut(s) 74, 80, 101, 186, 209, 213
Lsp1109I GCAGC 2 cut(s) 193, 295
LweI GCATC 2 cut(s) 189, 270
MboII GAAGA 2 cut(s) 76, 127
MhlI GDGCHC 1 cut(s) 240
MluCI AATT 1 cut(s) 23
MmeI TCCRAC 2 cut(s) 144, 153
MnlI CCTC 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 275
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 196
MspR9I CCNGG 1 cut(s) 89
MvaI CCWGG 1 cut(s) 89
NlaIV GGNNCC 1 cut(s) 158
PceI AGGCCT 1 cut(s) 98
PfeI GAWTC 1 cut(s) 330
PkrI GCNGC 3 cut(s) 208, 233, 285
PpuMI RGGWCCY 1 cut(s) 156
Psp124BI GAGCTC 1 cut(s) 240
Psp5II RGGWCCY 1 cut(s) 156
Psp6I CCWGG 1 cut(s) 87
PspGI CCWGG 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 158
PspPI GGNCC 1 cut(s) 156
PspPPI RGGWCCY 1 cut(s) 156
RseI CAYNNNNRTG 1 cut(s) 275
SacI GAGCTC 1 cut(s) 240
SatI GCNGC 3 cut(s) 207, 232, 284
Sau96I GGNCC 1 cut(s) 156
ScrFI CCNGG 1 cut(s) 89
SduI GDGCHC 1 cut(s) 240
SetI ASST 5 cut(s) 75, 158, 167, 178, 240
SfaNI GCATC 2 cut(s) 189, 270
SinI GGWCC 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 275
Sse9I AATT 1 cut(s) 23
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 2 cut(s) 225, 231
SstI GAGCTC 1 cut(s) 240
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 1 cut(s) 87
StyI CCWWGG 1 cut(s) 99
TaqI TCGA 1 cut(s) 328
TasI AATT 1 cut(s) 23
TauI GCSGC 1 cut(s) 234
TfiI GAWTC 1 cut(s) 330
TseI GCWGC 2 cut(s) 206, 283
TspGWI ACGGA 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 156
XagI CCTNNNNNAGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.