Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003156

Physical Location & Seq

Genomic Coordinates Reverse (-)

15133158 .. 15139531

Gene Structure 6374 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1075100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1869 bp

ATGAGCCGGGCACCACCAGATCTTTCTTCCCCAATTTCAATGGCCAGCACTACTACTCACATTAGCAACGGCGAACACGAACCGCCGACGAAGCCGCCCGCATTCACCTCCACCGCTTCCTTCAGGGCTCGCAAGAAGACCAATGCTTCCGCCGGAATCTCCCGTGCCGCCTCCGATGTCGATGACATCGTCACTCTCATGCACGGCTCCGATCCAGTCCGTGTTGAGCTCAACCGCCTCGAAAATGACCTCCGAGATAAAGACAGGGAGTTGGGAGATGCTGTGGCGGAAATTAAGTCTTTAAGAAATTCAGAGCGCCTTAAAGAAAAGGCGGTTGAAGAGTTGACAGATGAACTTAACAAAGTGGATGAAAAGCTTAAAGCAACTGAAGCTCTTTTGGAAAGCAAGAATCTTGAGATTAAGAAAGTAAATGATGAGAAAAAAGCAGCTTTGGCTGCGCAATTTGCAGCAGAAGCCACACTTCGAAGAGTTCACGCTGCACAGAAAGATGATGAAATGCCACCCATTGAAGCCATCATTGCTCCACTGGAGGCAGAGCTTAAGCTTGCTAGGCAGGAGGTAACCAAGTTGCAGGATGACAACAGAGCACTTGATCGGCTTACTAAATCCAAAGAGGCTGCTCTGGTTGACACCGAGAGAACTGTTCAGATTGCTTTGGCAAAAGCATCCTTGGTTGACGATCTGCAAAACAAAAATCAAGAGCTAATGAAACAGATTGAAATATGCCAGGAGGAGAACAAAATACTTGACAAAATGCACAGACAGAAGGTAGCTGAGGTTGAGAAGCTGACACAAACTGTTCGAGAACTTGAGGAGGCTGTTTTAGCTGGTGGAGCTGCTGCCAATGCTGTGCGTGATTACCAGCGGAAAGTGCAGGAGATGAATGAGGAGAAAAAGCTATTGGAGCGGGAGGTGGCTCGTGCAAAGGTCTCTGCAAATCGGGTTGCCACTGTTGTTGCAAATGACTGGAAAGACTCCAGTGACAAAGTCATGCCCGTAAAGCAATGGCTTGAAGAAAGGAAATTTTTTCAGGGAGAAATGCAACAGCTTAGAGACAAACTGGCTGTAGCTGAGCGTACTGCAAAGGCAGAAGCACAATTGAAGGAAAAATACCAATTAAGATTTAAGGTTTTGGAGGATAAGTTAAAAGCATCTAATGGTAAATCGCGTGCGGCTCCAGATGCAAAAATCACAAGCAACAGGCCTTCAAGGCGTCAGTCTCTTGGTGGAGCTGAGAACATTTCTAAATCGTCCTCTAATGGCTATCTATCTAGGACAAAATCAAACTTACAATCTGGTTTCAACCGTTCCAACAGTGCCACTGCAATATTGAAACAAGCCACAGTTTCATCTAGATCGTTTGATGGTGGTAGTAGATTGCTAGACAGAGATAAGCTAATCCCAGATGCAACTGGGAAAGATTGCACGCCTAACAGTGCCAATGATCAGACTCGAATGAATGAGGTAGTAGGTAGACATGAGGAGAGCTCAAATGGAACCTTGGCTGAACAGTCCAGGAGAGAACATGAAGATTATGTTTCTGGAGTACTGTATGATATGTTACAGAAAGAGGTTGTATCTTTAAGGAAAGCTTGCCACGAGAAAGATCAAACCCTCAAAGACAAGGATGATGCAATTGAGATGTTGGCAAAGAAGGTGGATACACTTAACAAAGCAATGGAGGTCGAGGCCAAAAAGATGCGGAGAGAAGTAGCTGCAATGGAAAAAGAAGTCTCTTCTATGCGTGTCACTAAGGAGCATGATCAGCGGACACGGCGCGTAAGTGCTCCCCGGGGGGCACTAAATAGTTCTCATACACTTTCTTCAAGAAATGCACAGAGGTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005874 GO:0006996 GO:0007010 GO:0008017 GO:0008092 GO:0008150 GO:0009987 GO:0015630 GO:0015631 GO:0016043 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0071840 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP70

Protein Analysis

623

Amino Acids

69.49

Weight (kDa)

8.96

Isoelectric Point (pI)

46.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP70	PF07058	61 - 612	9.4e-286	Microtubule-associated protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0003156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14840 AT1G14840 AT1G24764 AT1G24764 AT1G24764 AT1G68060 AT2G01750 AT2G01750
fragaria_vesca	FvH4_4g33511 FvH4_4g33511 FvH4_4g33511
malus_domestica	MD00G1075100.v1.1 MD12G1095700.v1.1 MD13G1020100.v1.1 MD16G1018200.v1.1
prunus_persica	Prupe.1G333700_v2.0.a1 Prupe.7G032400_v2.0.a1 Prupe.7G032700_v2.0.a1
pyrus_communis	pycom12g08260 pycom13g01700 pycom14g08120 pycom16g01650
rosa_chinensis	RchiOBHm_Chr3g0480111 RchiOBHm_Chr4g0443021
rosa_laevigata	RLG00000005962
rosa_multiflora	Rmu_sc0006604.1_g000002 Rmu_ssc0000204.1_g000001
rosa_roxburghii	Rroxscaffold_5G00383650
rosa_rugosa	Rorug04G0343800 Rorug04G0343800
rosa_samantha	Rh3AG231900 Rh3AG232000 Rh4AG398000 Rh4BG409500 Rh4CG425000 Rh4DG404300
rosa_wichuraiana	Rw4G034280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	459
AccB1I	GGYRCC	1 cut(s)	10
AccBSI	CCGCTC	1 cut(s)	928
AccI	GTMKAC	1 cut(s)	1495
AccII	CGCG	2 cut(s)	1189, 1800
AclWI	GGATC	1 cut(s)	206
AcoI	YGGCCR	1 cut(s)	42
AcsI	RAATTY	2 cut(s)	307, 1043
AcuI	CTGAAG	2 cut(s)	106, 408
AcyI	GRCGYC	1 cut(s)	1234
AfaI	GTAC	2 cut(s)	1099, 1569
AflII	CTTAAG	1 cut(s)	560
AjnI	CCWGG	2 cut(s)	747, 1535
AjuI	GAANNNNNNNTTGG	2 cut(s)	905, 937
Alw21I	GWGCWC	4 cut(s)	231, 610, 1511, 1810
Alw26I	GTCTC	4 cut(s)	955, 1068, 1245, 1759
AlwI	GGATC	1 cut(s)	206
Ama87I	CYCGRG	1 cut(s)	1812
AoxI	GGCC	3 cut(s)	42, 1223, 1710
ApeKI	GCWGC	8 cut(s)	446, 455, 467, 497, 638, 857, 860, 1736
ApoI	RAATTY	2 cut(s)	307, 1043
AspLEI	GCGC	3 cut(s)	318, 460, 1800
AsuC2I	CCSGG	3 cut(s)	8, 1813, 1814
AsuHPI	GGTGA	1 cut(s)	97
AsuII	TTCGAA	1 cut(s)	484
AvaI	CYCGRG	1 cut(s)	1812
BaeGI	GKGCMC	2 cut(s)	13, 1822
BalI	TGGCCA	1 cut(s)	44
BanI	GGYRCC	1 cut(s)	10
BanII	GRGCYC	3 cut(s)	130, 231, 1511
BarI	GAAGNNNNNNTAC	2 cut(s)	1115, 1147
BauI	CACGAG	2 cut(s)	939, 1619
BbsI	GAAGAC	1 cut(s)	143
Bbv12I	GWGCWC	4 cut(s)	231, 610, 1511, 1810
BbvCI	CCTCAGC	1 cut(s)	795
BbvI	GCAGC	8 cut(s)	442, 458, 479, 484, 625, 844, 847, 1723
BccI	CCATC	2 cut(s)	542, 1379
BceAI	ACGGC	3 cut(s)	85, 220, 1811
BciT130I	CCWGG	2 cut(s)	749, 1537
BciVI	GTATCC	1 cut(s)	1675
BclI	TGATCA	2 cut(s)	1465, 1783
BcnI	CCSGG	3 cut(s)	8, 1813, 1814
BcoDI	GTCTC	4 cut(s)	955, 1068, 1245, 1759
BfaI	CTAG	4 cut(s)	570, 1293, 1374, 1403
BfmI	CTRYAG	1 cut(s)	1086
BfoI	RGCGCY	1 cut(s)	319
BfrI	CTTAAG	1 cut(s)	560
BfuI	GTATCC	1 cut(s)	1675
BglI	GCCNNNNNGGC	1 cut(s)	1231
BglII	AGATCT	1 cut(s)	19
BlpI	GCTNAGC	1 cut(s)	1092
BmcAI	AGTACT	1 cut(s)	1569
Bme1390I	CCNGG	5 cut(s)	8, 749, 1537, 1813, 1814
BmeT110I	CYCGRG	1 cut(s)	1812
BmiI	GGNNCC	4 cut(s)	12, 208, 1197, 1519
BmrFI	CCNGG	5 cut(s)	8, 749, 1537, 1813, 1814
BmrI	ACTGGG	1 cut(s)	1443
BmsI	GCATC	7 cut(s)	268, 695, 1181, 1192, 1417, 1642, 1710
BmuI	ACTGGG	1 cut(s)	1443
BpiI	GAAGAC	1 cut(s)	143
BplI	GAGNNNNNCTC	2 cut(s)	1493, 1525
BpmI	CTGGAG	4 cut(s)	569, 982, 1182, 1584
Bpu10I	CCTNAGC	1 cut(s)	795
Bpu1102I	GCTNAGC	1 cut(s)	1092
Bpu14I	TTCGAA	1 cut(s)	484
BpuEI	CTTGAG	2 cut(s)	434, 851
BpuMI	CCSGG	3 cut(s)	8, 1813, 1814
BsaHI	GRCGYC	1 cut(s)	1234
BsaI	GGTCTC	1 cut(s)	955
BsaJI	CCNNGG	5 cut(s)	690, 1521, 1811, 1812, 1813
BsaXI	ACNNNNNCTCC	4 cut(s)	267, 297, 1148, 1178
Bse1I	ACTGG	6 cut(s)	215, 552, 992, 999, 1086, 1438
Bse3DI	GCAATG	4 cut(s)	537, 1031, 1704, 1746
BseBI	CCWGG	2 cut(s)	749, 1537
BseDI	CCNNGG	5 cut(s)	690, 1521, 1811, 1812, 1813
BseGI	GGATG	4 cut(s)	373, 601, 686, 1654
BseMI	GCAATG	4 cut(s)	537, 1031, 1704, 1746
BseMII	CTCAG	3 cut(s)	786, 1083, 1245
BseNI	ACTGG	6 cut(s)	215, 552, 992, 999, 1086, 1438
BseRI	GAGGAG	4 cut(s)	767, 848, 923, 1517
BseSI	GKGCMC	2 cut(s)	13, 1822
BseXI	GCAGC	8 cut(s)	442, 458, 479, 484, 625, 844, 847, 1723
BsgI	GTGCAG	2 cut(s)	483, 914
Bsh1236I	CGCG	2 cut(s)	1189, 1800
BshFI	GGCC	3 cut(s)	44, 1225, 1712
BshNI	GGYRCC	1 cut(s)	10
BsiHKAI	GWGCWC	4 cut(s)	231, 610, 1511, 1810
BsiHKCI	CYCGRG	1 cut(s)	1812
BsiSI	CCGG	3 cut(s)	7, 153, 1813
BsmAI	GTCTC	4 cut(s)	955, 1068, 1245, 1759
BsmI	GAATGC	1 cut(s)	101
BsnI	GGCC	3 cut(s)	44, 1225, 1712
Bso31I	GGTCTC	1 cut(s)	955
BsoBI	CYCGRG	1 cut(s)	1812
Bsp119I	TTCGAA	1 cut(s)	484
Bsp1286I	GDGCHC	7 cut(s)	13, 130, 231, 610, 1511, 1810, 1822
Bsp143I	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
Bsp1720I	GCTNAGC	1 cut(s)	1092
BspANI	GGCC	3 cut(s)	44, 1225, 1712
BspCNI	CTCAG	3 cut(s)	787, 1084, 1246
BspFNI	CGCG	2 cut(s)	1189, 1800
BspLI	GGNNCC	4 cut(s)	12, 208, 1197, 1519
BspPI	GGATC	1 cut(s)	206
BspT104I	TTCGAA	1 cut(s)	484
BspT107I	GGYRCC	1 cut(s)	10
BspTI	CTTAAG	1 cut(s)	560
BspTNI	GGTCTC	1 cut(s)	955
BsrBI	CCGCTC	1 cut(s)	928
BsrDI	GCAATG	4 cut(s)	537, 1031, 1704, 1746
BsrI	ACTGG	6 cut(s)	215, 552, 992, 999, 1086, 1438
BssECI	CCNNGG	5 cut(s)	690, 1521, 1811, 1812, 1813
BssMI	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
BssNI	GRCGYC	1 cut(s)	1234
BssSI	CACGAG	2 cut(s)	939, 1619
BssT1I	CCWWGG	2 cut(s)	690, 1521
Bst2BI	CACGAG	2 cut(s)	939, 1619
Bst2UI	CCWGG	2 cut(s)	749, 1537
Bst4CI	ACNGT	9 cut(s)	664, 820, 973, 1328, 1337, 1366, 1457, 1533, 1572
Bst6I	CTCTTC	3 cut(s)	333, 481, 1762
BstACI	GRCGYC	1 cut(s)	1234
BstAFI	CTTAAG	1 cut(s)	560
BstBI	TTCGAA	1 cut(s)	484
BstC8I	GCNNGC	7 cut(s)	46, 99, 130, 567, 1191, 1448, 1615
BstDEI	CTNAG	5 cut(s)	795, 1070, 1092, 1254, 1773
BstEII	GGTNACC	1 cut(s)	580
BstF5I	GGATG	4 cut(s)	373, 601, 686, 1654
BstFNI	CGCG	2 cut(s)	1189, 1800
BstH2I	RGCGCY	1 cut(s)	319
BstHHI	GCGC	3 cut(s)	318, 460, 1800
BstKTI	GATC	8 cut(s)	22, 214, 616, 703, 1379, 1468, 1630, 1786
BstMAI	GTCTC	4 cut(s)	955, 1068, 1245, 1759
BstMBI	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
BstNI	CCWGG	2 cut(s)	749, 1537
BstPI	GGTNACC	1 cut(s)	580
BstSCI	CCNGG	5 cut(s)	6, 747, 1535, 1811, 1812
BstSFI	CTRYAG	1 cut(s)	1086
BstSLI	GKGCMC	2 cut(s)	13, 1822
BstUI	CGCG	2 cut(s)	1189, 1800
BstV1I	GCAGC	8 cut(s)	442, 458, 479, 484, 625, 844, 847, 1723
BstV2I	GAAGAC	1 cut(s)	143
BstX2I	RGATCY	1 cut(s)	19
BstYI	RGATCY	1 cut(s)	19
BsuI	GTATCC	1 cut(s)	1675
BsuRI	GGCC	3 cut(s)	44, 1225, 1712
BtsCI	GGATG	4 cut(s)	373, 601, 686, 1654
BtsI	GCAGTG	1 cut(s)	1341
BtsIMutI	CAGTG	6 cut(s)	545, 969, 1006, 1341, 1342, 1462
Cac8I	GCNNGC	7 cut(s)	46, 99, 130, 567, 1191, 1448, 1615
CfoI	GCGC	3 cut(s)	318, 460, 1800
Cfr9I	CCCGGG	1 cut(s)	1812
CseI	GACGC	1 cut(s)	1223
Csp6I	GTAC	2 cut(s)	1098, 1568
CspCI	CAANNNNNGTGG	4 cut(s)	958, 993, 1659, 1694
CviAII	CATG	5 cut(s)	199, 1012, 1499, 1547, 1781
CviQI	GTAC	2 cut(s)	1098, 1568
DdeI	CTNAG	5 cut(s)	795, 1070, 1092, 1254, 1773
DpnI	GATC	8 cut(s)	21, 213, 615, 702, 1378, 1467, 1629, 1785
DpnII	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
EaeI	YGGCCR	1 cut(s)	42
Eam1104I	CTCTTC	3 cut(s)	333, 481, 1762
EarI	CTCTTC	3 cut(s)	333, 481, 1762
EciI	GGCGGA	2 cut(s)	139, 302
Ecl136II	GAGCTC	2 cut(s)	229, 1509
Eco130I	CCWWGG	2 cut(s)	690, 1521
Eco147I	AGGCCT	1 cut(s)	1225
Eco24I	GRGCYC	3 cut(s)	130, 231, 1511
Eco31I	GGTCTC	1 cut(s)	955
Eco53kI	GAGCTC	2 cut(s)	229, 1509
Eco57I	CTGAAG	2 cut(s)	106, 408
Eco88I	CYCGRG	1 cut(s)	1812
Eco91I	GGTNACC	1 cut(s)	580
EcoICRI	GAGCTC	2 cut(s)	229, 1509
EcoO65I	GGTNACC	1 cut(s)	580
EcoRII	CCWGG	2 cut(s)	747, 1535
EcoT14I	CCWWGG	2 cut(s)	690, 1521
EcoT38I	GRGCYC	3 cut(s)	130, 231, 1511
ErhI	CCWWGG	2 cut(s)	690, 1521
FaeI	CATG	5 cut(s)	202, 1015, 1502, 1550, 1784
FalI	AAGNNNNNCTT	4 cut(s)	465, 497, 1597, 1629
FatI	CATG	5 cut(s)	198, 1011, 1498, 1546, 1780
FauI	CCCGC	2 cut(s)	106, 921
FbaI	TGATCA	2 cut(s)	1465, 1783
FblI	GTMKAC	1 cut(s)	1495
FokI	GGATG	4 cut(s)	380, 608, 673, 1661
FriOI	GRGCYC	3 cut(s)	130, 231, 1511
FspBI	CTAG	4 cut(s)	570, 1293, 1374, 1403
FspI	TGCGCA	1 cut(s)	459
GlaI	GCGC	3 cut(s)	317, 459, 1799
GsuI	CTGGAG	4 cut(s)	569, 982, 1182, 1584
HaeII	RGCGCY	1 cut(s)	319
HaeIII	GGCC	3 cut(s)	44, 1225, 1712
HapII	CCGG	3 cut(s)	7, 153, 1813
HgaI	GACGC	1 cut(s)	1223
HhaI	GCGC	3 cut(s)	318, 460, 1800
Hin1I	GRCGYC	1 cut(s)	1234
Hin1II	CATG	5 cut(s)	202, 1015, 1502, 1550, 1784
Hin6I	GCGC	3 cut(s)	316, 458, 1798
HinP1I	GCGC	3 cut(s)	316, 458, 1798
HincII	GTYRAC	3 cut(s)	345, 649, 697
HindII	GTYRAC	3 cut(s)	345, 649, 697
HindIII	AAGCTT	3 cut(s)	374, 563, 1611
HinfI	GANTC	4 cut(s)	156, 409, 995, 1471
HpaII	CCGG	3 cut(s)	7, 153, 1813
HphI	GGTGA	1 cut(s)	97
Hpy166II	GTNNAC	5 cut(s)	345, 493, 649, 697, 1496
Hpy188I	TCNGA	6 cut(s)	175, 211, 254, 313, 669, 1470
Hpy188III	TCNNGA	7 cut(s)	413, 719, 824, 1199, 1374, 1563, 1848
Hpy8I	GTNNAC	5 cut(s)	345, 493, 649, 697, 1496
Hpy99I	CGWCG	1 cut(s)	91
HpyAV	CCTTC	5 cut(s)	130, 781, 1117, 1236, 1669
HpyCH4III	ACNGT	9 cut(s)	664, 820, 973, 1328, 1337, 1366, 1457, 1533, 1572
HpyF3I	CTNAG	5 cut(s)	795, 1070, 1092, 1254, 1773
Hsp92I	GRCGYC	1 cut(s)	1234
Hsp92II	CATG	5 cut(s)	202, 1015, 1502, 1550, 1784
HspAI	GCGC	3 cut(s)	316, 458, 1798
Ksp22I	TGATCA	2 cut(s)	1465, 1783
Kzo9I	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
LmnI	GCTCC	8 cut(s)	212, 547, 854, 925, 1201, 1250, 1777, 1813
Lsp1109I	GCAGC	8 cut(s)	442, 458, 479, 484, 625, 844, 847, 1723
LweI	GCATC	7 cut(s)	268, 695, 1181, 1192, 1417, 1642, 1710
MaeI	CTAG	4 cut(s)	570, 1293, 1374, 1403
MaeIII	GTNAC	5 cut(s)	190, 580, 1001, 1581, 1768
MalI	GATC	8 cut(s)	21, 213, 615, 702, 1378, 1467, 1629, 1785
MbiI	CCGCTC	1 cut(s)	928
MboI	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
MboII	GAAGA	8 cut(s)	18, 148, 350, 498, 1046, 1562, 1749, 1836
MfeI	CAATTG	2 cut(s)	1118, 1656
MflI	RGATCY	1 cut(s)	19
MhlI	GDGCHC	7 cut(s)	13, 130, 231, 610, 1511, 1810, 1822
MlsI	TGGCCA	1 cut(s)	44
MluCI	AATT	8 cut(s)	33, 291, 307, 461, 1043, 1118, 1136, 1656
MluNI	TGGCCA	1 cut(s)	44
MlyI	GAGTC	2 cut(s)	989, 1465
MmeI	TCCRAC	1 cut(s)	1356
Mox20I	TGGCCA	1 cut(s)	44
MscI	TGGCCA	1 cut(s)	44
MslI	CAYNNNNRTG	1 cut(s)	197
Msp20I	TGGCCA	1 cut(s)	44
MspA1I	CMGCKG	2 cut(s)	886, 1789
MspCI	CTTAAG	1 cut(s)	560
MspI	CCGG	3 cut(s)	7, 153, 1813
MspR9I	CCNGG	5 cut(s)	8, 749, 1537, 1813, 1814
MunI	CAATTG	2 cut(s)	1118, 1656
Mva1269I	GAATGC	1 cut(s)	101
MvaI	CCWGG	2 cut(s)	749, 1537
MvnI	CGCG	2 cut(s)	1189, 1800
NciI	CCSGG	3 cut(s)	8, 1813, 1814
NdeII	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
NlaIII	CATG	5 cut(s)	202, 1015, 1502, 1550, 1784
NlaIV	GGNNCC	4 cut(s)	12, 208, 1197, 1519
NmuCI	GTSAC	3 cut(s)	190, 1001, 1768
NsbI	TGCGCA	1 cut(s)	459
NspV	TTCGAA	1 cut(s)	484
PceI	AGGCCT	1 cut(s)	1225
PcsI	WCGNNNNNNNCGW	2 cut(s)	75, 186
PctI	GAATGC	1 cut(s)	101
PfeI	GAWTC	2 cut(s)	156, 409
PflFI	GACNNNGTC	2 cut(s)	188, 1007
PfoI	TCCNGGA	1 cut(s)	1535
PleI	GAGTC	2 cut(s)	989, 1465
PpsI	GAGTC	2 cut(s)	989, 1465
Psp124BI	GAGCTC	2 cut(s)	231, 1511
Psp6I	CCWGG	2 cut(s)	747, 1535
PspEI	GGTNACC	1 cut(s)	580
PspGI	CCWGG	2 cut(s)	747, 1535
PspN4I	GGNNCC	4 cut(s)	12, 208, 1197, 1519
PsuI	RGATCY	1 cut(s)	19
PsyI	GACNNNGTC	2 cut(s)	188, 1007
RsaI	GTAC	2 cut(s)	1099, 1569
RsaNI	GTAC	2 cut(s)	1098, 1568
RseI	CAYNNNNRTG	1 cut(s)	197
SacI	GAGCTC	2 cut(s)	231, 1511
Sau3AI	GATC	8 cut(s)	19, 211, 613, 700, 1376, 1465, 1627, 1783
ScaI	AGTACT	1 cut(s)	1569
SchI	GAGTC	2 cut(s)	989, 1465
ScrFI	CCNGG	5 cut(s)	8, 749, 1537, 1813, 1814
SduI	GDGCHC	7 cut(s)	13, 130, 231, 610, 1511, 1810, 1822
SfaNI	GCATC	7 cut(s)	268, 695, 1181, 1192, 1417, 1642, 1710
SfcI	CTRYAG	1 cut(s)	1086
SfuI	TTCGAA	1 cut(s)	484
SmaI	CCCGGG	1 cut(s)	1814
SmiMI	CAYNNNNRTG	1 cut(s)	197
SmlI	CTYRAG	3 cut(s)	413, 560, 830
SmoI	CTYRAG	3 cut(s)	413, 560, 830
Sse9I	AATT	8 cut(s)	33, 291, 307, 461, 1043, 1118, 1136, 1656
SseBI	AGGCCT	1 cut(s)	1225
SspI	AATATT	1 cut(s)	1350
SspMI	CTAG	4 cut(s)	570, 1293, 1374, 1403
SstI	GAGCTC	2 cut(s)	231, 1511
StuI	AGGCCT	1 cut(s)	1225
StyD4I	CCNGG	5 cut(s)	6, 747, 1535, 1811, 1812
StyI	CCWWGG	2 cut(s)	690, 1521
TaaI	ACNGT	9 cut(s)	664, 820, 973, 1328, 1337, 1366, 1457, 1533, 1572
TaqI	TCGA	6 cut(s)	180, 240, 484, 823, 1474, 1707
TasI	AATT	8 cut(s)	33, 291, 307, 461, 1043, 1118, 1136, 1656
TatI	WGTACW	1 cut(s)	1567
TauI	GCSGC	3 cut(s)	97, 170, 1196
TfiI	GAWTC	2 cut(s)	156, 409
TscAI	CASTG	6 cut(s)	552, 976, 1006, 1342, 1348, 1462
TseFI	GTSAC	3 cut(s)	190, 1001, 1768
TseI	GCWGC	8 cut(s)	446, 455, 467, 497, 638, 857, 860, 1736
Tsp45I	GTSAC	3 cut(s)	190, 1001, 1768
TspDTI	ATGAA	8 cut(s)	366, 384, 528, 743, 917, 1359, 1493, 1563
TspGWI	ACGGA	1 cut(s)	209
TspMI	CCCGGG	1 cut(s)	1812
TspRI	CASTG	6 cut(s)	552, 976, 1006, 1342, 1348, 1462
Tth111I	GACNNNGTC	2 cut(s)	188, 1007
Vha464I	CTTAAG	1 cut(s)	560
XapI	RAATTY	2 cut(s)	307, 1043
XbaI	TCTAGA	1 cut(s)	1373
XmaI	CCCGGG	1 cut(s)	1812
XmiI	GTMKAC	1 cut(s)	1495
XspI	CTAG	4 cut(s)	570, 1293, 1374, 1403
ZrmI	AGTACT	1 cut(s)	1569

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.