Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0003156

Physical Location & Seq

Genomic Coordinates Forward (+)

1267306 .. 1271481

Gene Structure 4176 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1020100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGGAGGAGGCTTTCGGCGACGGAGGTTATGCTCCGATGACGGAGTACAGTAACGGACCGGTTCATGGGGGTGCGCCGACACCGCCGCCGCTGATGGTGTCGGGATCGTTCAAGGAGGGGAAGAGCTCATCGCGGAGGAGAGGATTCAGGAGGCCGAGTATGGACGGCGACGAGCTCATAAACCTCTTGCACGGCTCGGATCCGGTCAAGGTCGAGCTCAATCGGCTCGAGAATGAAGTCAGAGATAAGGACCGGGAGTTAGGAGAGGCACAGGCTCAGATCAAGGCTCTCAAGCTCTCTGATAGAGCCAGAGAAAAGGCTTGTGAAGAGCTCACTGATGAGTTGTCGAAGGTGGAAGAAAAGCTGAAGTTGACAGAATCACTTCTAGAAAGCAGAAATCTTGAAATTAAGAAAATCAATGATGAGAAGAAAGCTTCCATGGCTGCTCAGTTCGCAGCTGAAGCTACTCTACGGAGGGTTCATGCTGCGCAAAAGGATGATGATATGCCTCCTATTGAAGCCATTCTTGCACCTCTGGAGGCAGAGCTCAAGCTTGCCCGGCAAGAGATTGCAAAACTTCAAGATGATAACAAAGCTTTGGATCGCCTTACCAAATCAAAAGAAGCAGCTTTACTTGAGGCTGAGAGGACTGTTCAGGTTGCCTTGGCTAAGGCCTCCATGGTGGATGATCTTCAGAATAAAAACCAAGAGTTGATGAAACAGATAGAAATTTGTCAGGAAGAAAATAAAATTTTAGACAAGATGCATAGACAAAAGGTTGCTGAGGTTGAAAAGCTTACTCAGACTGTGCGGGAGTTGGAAGAGGCTGTTCTTTCGGGTGGTGCAGCGGCAAATGCTGTGAGGGATTATCAGCGGAAGTTTCAGGAGATGAATGAGGAAAGGAAAACTCTGGACCGGGAGTTGGCCCGTGCAAAAGTAACAGCAAATAGAGTAGCTGTAGTGGTAGCAAATGAGTGGAAAGATGCTAATGACAAAGTGATGCCTGTAAAACAATGGCTTGAAGAACGGAGATTTTTGCAGGGAGAAATGCAACAACTTCGTGACAAGCTTGCCATAACTGAGCGAGCTGCCAAGTCTGAAGCTCAGTTGAAAGAAAAATATCACATGCGACTTAAAGTGCTTGAGGAGAGTGTGAGAGGTAACAGTATTAATCGCAGCACAGTAGAGGGAAGAAGTACAAGCAATGGGCCCTCGCGACGTCAGTCCCTGGGTGGAGCTGATAACGCTCCAAAATTAAGCTCCAATGGGTTTTCATCCAAGAGAACTCCCGTCAGGTCTTTGTCCTCCAGCACCAGTTCAGTGTTAAAGCATGCTAAGGGCACATCAAAATCATTTGATGGTGGTACAAGATCATTGGACAGGGGTAAGGTTCACTTGAACGGTACAAGCCCCAGTTTCTCTGTCAACCAATCTTGTGAGGGAACAAAGGATGGTGAAGCTCAAACTAACTGGAAAGGAAATTCAGATGATAAGCCTGAGTTCTCGACAGTGGATACGGAGGATAGTGTCCCTGGGGTTTTGTATGATTTGTTGCAGAAAGAGGTTGTAGCGTTGAGGAAAGCTGGTCATGAGAAAGATCAAAGCCTGAAAGACAAGGATGATGCTATTGAGATGTTAGCCAAGAAGGTAGATACCTTGACTAAAGCAATGGAGGTTGAAGCAAAGAAAATGAGAAGAGAAGTTGCTTCCATGGAGAAGGAGGTAGCTGCCATGCGTGTGGACAAAGAGCATGAGAATAGGGCCAAGCGGTTTGGTCATACCAAAAGTTCTGTCAGCAGTGCTCAGGTTCTCTCTGGAAGGGGTCTCGCGCGAAGTGGGTTAACCCGCAGCACCCAATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003002 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005874 GO:0005881 GO:0005938 GO:0006996 GO:0007010 GO:0007275 GO:0007389 GO:0008017 GO:0008092 GO:0008150 GO:0009832 GO:0009987 GO:0010005 GO:0010051 GO:0015630 GO:0015631 GO:0016043 GO:0030863 GO:0030981 GO:0032501 GO:0032502 GO:0042546 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0048856 GO:0055028 GO:0071554 GO:0071669 GO:0071840 GO:0071944 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MAP70

Protein Analysis

620

Amino Acids

68.84

Weight (kDa)

8.68

Isoelectric Point (pI)

45.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MAP70	PF07058	57 - 603	8.7e-291	Microtubule-associated protein 70

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003156)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G14840 AT1G14840 AT1G24764 AT1G24764 AT1G24764 AT1G68060 AT2G01750 AT2G01750
fragaria_vesca	FvH4_4g33511 FvH4_4g33511 FvH4_4g33511
malus_domestica	MD00G1075100.v1.1 MD12G1095700.v1.1 MD13G1020100.v1.1 MD16G1018200.v1.1
prunus_persica	Prupe.1G333700_v2.0.a1 Prupe.7G032400_v2.0.a1 Prupe.7G032700_v2.0.a1
pyrus_communis	pycom12g08260 pycom13g01700 pycom14g08120 pycom16g01650
rosa_chinensis	RchiOBHm_Chr3g0480111 RchiOBHm_Chr4g0443021
rosa_laevigata	RLG00000005962
rosa_multiflora	Rmu_sc0006604.1_g000002 Rmu_ssc0000204.1_g000001
rosa_roxburghii	Rroxscaffold_5G00383650
rosa_rugosa	Rorug04G0343800 Rorug04G0343800
rosa_samantha	Rh3AG231900 Rh3AG232000 Rh4AG398000 Rh4BG409500 Rh4CG425000 Rh4DG404300
rosa_wichuraiana	Rw4G034280

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1222
Acc16I	TGCGCA	1 cut(s)	489
AccII	CGCG	4 cut(s)	133, 1216, 1829, 1831
AciI	CCGC	9 cut(s)	83, 86, 89, 133, 811, 848, 874, 1768, 1846
AclWI	GGATC	4 cut(s)	112, 194, 207, 609
AcsI	RAATTY	3 cut(s)	729, 750, 1480
AcuI	CTGAAG	4 cut(s)	386, 480, 677, 1119
AcyI	GRCGYC	1 cut(s)	1219
AfaI	GTAC	4 cut(s)	47, 1198, 1366, 1405
AfiI	CCNNNNNNNGG	2 cut(s)	65, 922
AgeI	ACCGGT	1 cut(s)	58
AgsI	TTSAA	9 cut(s)	112, 404, 518, 581, 791, 1022, 1111, 1399, 1679
AjnI	CCWGG	2 cut(s)	1227, 1531
Alw21I	GWGCWC	6 cut(s)	128, 177, 219, 333, 549, 1804
Alw26I	GTCTC	1 cut(s)	1829
AlwI	GGATC	4 cut(s)	112, 194, 207, 609
AlwNI	CAGNNNCTG	1 cut(s)	1228
Ama87I	CYCGRG	1 cut(s)	227
AoxI	GGCC	5 cut(s)	152, 672, 924, 1208, 1761
ApaI	GGGCCC	1 cut(s)	1212
ApeKI	GCWGC	9 cut(s)	443, 455, 485, 626, 845, 1088, 1176, 1727, 1848
ApoI	RAATTY	3 cut(s)	729, 750, 1480
ArsI	GACNNNNNNTTYG	2 cut(s)	1776, 1808
AseI	ATTAAT	1 cut(s)	1170
AsiGI	ACCGGT	1 cut(s)	58
Asp700I	GAANNNNTTC	2 cut(s)	381, 522
AspLEI	GCGC	3 cut(s)	76, 490, 1831
AspS9I	GGNCC	7 cut(s)	56, 250, 913, 925, 1208, 1209, 1761
AsuC2I	CCSGG	3 cut(s)	254, 559, 917
AsuHPI	GGTGA	1 cut(s)	1466
AvaI	CYCGRG	1 cut(s)	227
AvaII	GGWCC	3 cut(s)	56, 250, 913
BaeGI	GKGCMC	2 cut(s)	1212, 1343
BamHI	GGATCC	1 cut(s)	199
BanII	GRGCYC	6 cut(s)	128, 177, 219, 333, 549, 1212
BarI	GAAGNNNNNNTAC	4 cut(s)	453, 485, 615, 647
Bbv12I	GWGCWC	6 cut(s)	128, 177, 219, 333, 549, 1804
BbvCI	CCTCAGC	1 cut(s)	783
BbvI	GCAGC	8 cut(s)	430, 467, 472, 638, 857, 1075, 1188, 1714
BccI	CCATC	3 cut(s)	88, 1352, 1445
BceAI	ACGGC	2 cut(s)	181, 208
BcgI	CGANNNNNNTGC	2 cut(s)	1040, 1074
BciT130I	CCWGG	2 cut(s)	1229, 1533
BciVI	GTATCC	1 cut(s)	1507
BcnI	CCSGG	3 cut(s)	254, 559, 917
BcoDI	GTCTC	1 cut(s)	1829
BfaI	CTAG	1 cut(s)	386
BfmI	CTRYAG	1 cut(s)	957
BfuI	GTATCC	1 cut(s)	1507
Bme1390I	CCNGG	5 cut(s)	254, 559, 917, 1229, 1533
Bme18I	GGWCC	3 cut(s)	56, 250, 913
BmeT110I	CYCGRG	1 cut(s)	227
BmgT120I	GGNCC	7 cut(s)	56, 250, 913, 925, 1208, 1209, 1761
BmiI	GGNNCC	2 cut(s)	201, 1210
BmrFI	CCNGG	5 cut(s)	254, 559, 917, 1229, 1533
BmrI	ACTGGG	1 cut(s)	1407
BmsI	GCATC	4 cut(s)	753, 973, 990, 1612
BmuI	ACTGGG	1 cut(s)	1407
BoxI	GACNNNNGTC	1 cut(s)	1222
BpmI	CTGGAG	2 cut(s)	557, 1291
Bpu10I	CCTNAGC	4 cut(s)	669, 783, 1335, 1803
BpuEI	CTTGAG	4 cut(s)	275, 533, 656, 1163
BpuMI	CCSGG	3 cut(s)	254, 559, 917
BsaHI	GRCGYC	1 cut(s)	1219
BsaI	GGTCTC	1 cut(s)	1829
BsaJI	CCNNGG	8 cut(s)	438, 663, 678, 1227, 1228, 1531, 1532, 1710
BsaWI	WCCGGW	2 cut(s)	58, 202
BsaXI	ACNNNNNCTCC	2 cut(s)	142, 172
Bsc4I	CCNNNNNNNGG	2 cut(s)	65, 922
Bse118I	RCCGGY	1 cut(s)	58
Bse1I	ACTGG	3 cut(s)	1314, 1413, 1475
Bse3DI	GCAATG	2 cut(s)	1210, 1674
BseBI	CCWGG	2 cut(s)	1229, 1533
BseDI	CCNNGG	8 cut(s)	438, 663, 678, 1227, 1228, 1531, 1532, 1710
BseGI	GGATG	5 cut(s)	502, 691, 1274, 1456, 1624
BseLI	CCNNNNNNNGG	2 cut(s)	65, 922
BseMI	GCAATG	2 cut(s)	1210, 1674
BseMII	CTCAG	9 cut(s)	290, 461, 633, 774, 815, 1071, 1118, 1488, 1817
BseNI	ACTGG	3 cut(s)	1314, 1413, 1475
BseRI	GAGGAG	3 cut(s)	20, 151, 1160
BseSI	GKGCMC	2 cut(s)	1212, 1343
BseXI	GCAGC	8 cut(s)	430, 467, 472, 638, 857, 1075, 1188, 1714
BsgI	GTGCAG	1 cut(s)	864
Bsh1236I	CGCG	4 cut(s)	133, 1216, 1829, 1831
BshFI	GGCC	5 cut(s)	154, 674, 926, 1210, 1763
BshTI	ACCGGT	1 cut(s)	58
BsiHKAI	GWGCWC	6 cut(s)	128, 177, 219, 333, 549, 1804
BsiHKCI	CYCGRG	1 cut(s)	227
BsiSI	CCGG	5 cut(s)	59, 203, 253, 559, 916
BslFI	GGGAC	2 cut(s)	1210, 1514
BslI	CCNNNNNNNGG	2 cut(s)	65, 922
BsmAI	GTCTC	1 cut(s)	1829
BsmFI	GGGAC	2 cut(s)	1210, 1514
BsnI	GGCC	5 cut(s)	154, 674, 926, 1210, 1763
Bso31I	GGTCTC	1 cut(s)	1829
BsoBI	CYCGRG	1 cut(s)	227
Bsp120I	GGGCCC	1 cut(s)	1208
Bsp1286I	GDGCHC	8 cut(s)	128, 177, 219, 333, 549, 1212, 1343, 1804
Bsp143I	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
Bsp19I	CCATGG	3 cut(s)	438, 678, 1710
Bsp68I	TCGCGA	1 cut(s)	1216
BspACI	CCGC	9 cut(s)	83, 86, 89, 133, 811, 848, 874, 1768, 1846
BspANI	GGCC	5 cut(s)	154, 674, 926, 1210, 1763
BspCNI	CTCAG	9 cut(s)	289, 460, 634, 775, 814, 1072, 1117, 1489, 1816
BspFNI	CGCG	4 cut(s)	133, 1216, 1829, 1831
BspHI	TCATGA	1 cut(s)	1588
BspLI	GGNNCC	2 cut(s)	201, 1210
BspPI	GGATC	4 cut(s)	112, 194, 207, 609
BspQI	GCTCTTC	2 cut(s)	116, 321
BspTNI	GGTCTC	1 cut(s)	1829
BsrDI	GCAATG	2 cut(s)	1210, 1674
BsrFI	RCCGGY	1 cut(s)	58
BsrI	ACTGG	3 cut(s)	1314, 1413, 1475
BssAI	RCCGGY	1 cut(s)	58
BssECI	CCNNGG	8 cut(s)	438, 663, 678, 1227, 1228, 1531, 1532, 1710
BssMI	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
BssNI	GRCGYC	1 cut(s)	1219
BssT1I	CCWWGG	4 cut(s)	438, 663, 678, 1710
Bst2UI	CCWGG	2 cut(s)	1229, 1533
Bst4CI	ACNGT	7 cut(s)	50, 652, 808, 1166, 1183, 1403, 1510
Bst6I	CTCTTC	4 cut(s)	116, 321, 816, 1690
BstACI	GRCGYC	1 cut(s)	1219
BstC8I	GCNNGC	4 cut(s)	555, 1071, 1086, 1332
BstDSI	CCRYGG	3 cut(s)	438, 678, 1710
BstF5I	GGATG	5 cut(s)	502, 691, 1274, 1456, 1624
BstFNI	CGCG	4 cut(s)	133, 1216, 1829, 1831
BstHHI	GCGC	3 cut(s)	76, 490, 1831
BstKTI	GATC	7 cut(s)	107, 202, 282, 604, 691, 1373, 1600
BstMAI	GTCTC	1 cut(s)	1829
BstMBI	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
BstMWI	GCNNNNNNNGC	9 cut(s)	82, 223, 440, 452, 461, 527, 559, 854, 1244
BstNI	CCWGG	2 cut(s)	1229, 1533
BstNSI	RCATGY	2 cut(s)	1129, 1334
BstPAI	GACNNNNGTC	1 cut(s)	1222
BstSCI	CCNGG	5 cut(s)	252, 557, 915, 1227, 1531
BstSFI	CTRYAG	1 cut(s)	957
BstSLI	GKGCMC	2 cut(s)	1212, 1343
BstUI	CGCG	4 cut(s)	133, 1216, 1829, 1831
BstV1I	GCAGC	8 cut(s)	430, 467, 472, 638, 857, 1075, 1188, 1714
BstX2I	RGATCY	1 cut(s)	199
BstXI	CCANNNNNNTGG	1 cut(s)	1738
BstYI	RGATCY	1 cut(s)	199
BsuI	GTATCC	1 cut(s)	1507
BsuRI	GGCC	5 cut(s)	154, 674, 926, 1210, 1763
BtgI	CCRYGG	3 cut(s)	438, 678, 1710
BtgZI	GCGATG	1 cut(s)	114
BtsCI	GGATG	5 cut(s)	502, 691, 1274, 1456, 1624
BtsI	GCAGTG	1 cut(s)	1804
BtsIMutI	CAGTG	4 cut(s)	333, 1326, 1515, 1804
BtuMI	TCGCGA	1 cut(s)	1216
Cac8I	GCNNGC	4 cut(s)	555, 1071, 1086, 1332
CaiI	CAGNNNCTG	1 cut(s)	1228
CciI	TCATGA	1 cut(s)	1588
CfoI	GCGC	3 cut(s)	76, 490, 1831
Cfr10I	RCCGGY	1 cut(s)	58
Cfr13I	GGNCC	7 cut(s)	56, 250, 913, 925, 1208, 1209, 1761
CpoI	CGGWCCG	1 cut(s)	56
Csp6I	GTAC	4 cut(s)	46, 1197, 1365, 1404
CspAI	ACCGGT	1 cut(s)	58
CspI	CGGWCCG	1 cut(s)	56
CviQI	GTAC	4 cut(s)	46, 1197, 1365, 1404
DpnI	GATC	7 cut(s)	106, 201, 281, 603, 690, 1372, 1599
DpnII	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
Eam1104I	CTCTTC	4 cut(s)	116, 321, 816, 1690
EarI	CTCTTC	4 cut(s)	116, 321, 816, 1690
Ecl136II	GAGCTC	5 cut(s)	126, 175, 217, 331, 547
Eco130I	CCWWGG	4 cut(s)	438, 663, 678, 1710
Eco147I	AGGCCT	1 cut(s)	674
Eco24I	GRGCYC	6 cut(s)	128, 177, 219, 333, 549, 1212
Eco31I	GGTCTC	1 cut(s)	1829
Eco47I	GGWCC	3 cut(s)	56, 250, 913
Eco53kI	GAGCTC	5 cut(s)	126, 175, 217, 331, 547
Eco57I	CTGAAG	4 cut(s)	386, 480, 677, 1119
Eco88I	CYCGRG	1 cut(s)	227
EcoICRI	GAGCTC	5 cut(s)	126, 175, 217, 331, 547
EcoO109I	RGGNCCY	1 cut(s)	1209
EcoRII	CCWGG	2 cut(s)	1227, 1531
EcoT14I	CCWWGG	4 cut(s)	438, 663, 678, 1710
EcoT22I	ATGCAT	1 cut(s)	768
EcoT38I	GRGCYC	6 cut(s)	128, 177, 219, 333, 549, 1212
ErhI	CCWWGG	4 cut(s)	438, 663, 678, 1710
FalI	AAGNNNNNCTT	4 cut(s)	510, 542, 1379, 1411
FaqI	GGGAC	2 cut(s)	1210, 1514
FauI	CCCGC	2 cut(s)	804, 1853
FokI	GGATG	5 cut(s)	509, 698, 1261, 1463, 1631
FriOI	GRGCYC	6 cut(s)	128, 177, 219, 333, 549, 1212
FspBI	CTAG	1 cut(s)	386
FspI	TGCGCA	1 cut(s)	489
GlaI	GCGC	3 cut(s)	75, 489, 1830
GsuI	CTGGAG	2 cut(s)	557, 1291
HaeIII	GGCC	5 cut(s)	154, 674, 926, 1210, 1763
HapII	CCGG	5 cut(s)	59, 203, 253, 559, 916
HhaI	GCGC	3 cut(s)	76, 490, 1831
Hin1I	GRCGYC	1 cut(s)	1219
Hin6I	GCGC	3 cut(s)	74, 488, 1829
HinP1I	GCGC	3 cut(s)	74, 488, 1829
HincII	GTYRAC	3 cut(s)	372, 1426, 1842
HindII	GTYRAC	3 cut(s)	372, 1426, 1842
HindIII	AAGCTT	5 cut(s)	432, 551, 594, 794, 1067
HinfI	GANTC	2 cut(s)	144, 377
HpaI	GTTAAC	1 cut(s)	1842
HpaII	CCGG	5 cut(s)	59, 203, 253, 559, 916
HphI	GGTGA	1 cut(s)	1466
Hpy166II	GTNNAC	5 cut(s)	372, 1393, 1426, 1741, 1842
Hpy188I	TCNGA	9 cut(s)	36, 199, 242, 279, 301, 696, 804, 1099, 1486
Hpy8I	GTNNAC	5 cut(s)	372, 1393, 1426, 1741, 1842
Hpy99I	CGWCG	3 cut(s)	23, 173, 1221
HpyAV	CCTTC	4 cut(s)	343, 1639, 1711, 1812
HpyCH4III	ACNGT	7 cut(s)	50, 652, 808, 1166, 1183, 1403, 1510
HpyCH4IV	ACGT	1 cut(s)	1219
HpyCH4V	TGCA	9 cut(s)	190, 530, 572, 766, 845, 932, 1039, 1051, 1555
HpyF10VI	GCNNNNNNNGC	9 cut(s)	82, 223, 440, 452, 461, 527, 559, 854, 1244
HpySE526I	ACGT	1 cut(s)	1219
Hsp92I	GRCGYC	1 cut(s)	1219
HspAI	GCGC	3 cut(s)	74, 488, 1829
KspAI	GTTAAC	1 cut(s)	1842
Kzo9I	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
LguI	GCTCTTC	2 cut(s)	116, 321
LmnI	GCTCC	4 cut(s)	37, 1235, 1252, 1265
Lsp1109I	GCAGC	8 cut(s)	430, 467, 472, 638, 857, 1075, 1188, 1714
LweI	GCATC	4 cut(s)	753, 973, 990, 1612
MaeI	CTAG	1 cut(s)	386
MaeII	ACGT	1 cut(s)	1219
MaeIII	GTNAC	4 cut(s)	50, 937, 1061, 1160
MalI	GATC	7 cut(s)	106, 201, 281, 603, 690, 1372, 1599
MboI	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
MflI	RGATCY	1 cut(s)	199
MhlI	GDGCHC	8 cut(s)	128, 177, 219, 333, 549, 1212, 1343, 1804
MluCI	AATT	5 cut(s)	405, 729, 750, 1253, 1480
MmeI	TCCRAC	1 cut(s)	798
Mph1103I	ATGCAT	1 cut(s)	768
MroXI	GAANNNNTTC	2 cut(s)	381, 522
MseI	TTAA	6 cut(s)	408, 1134, 1170, 1256, 1325, 1841
MslI	CAYNNNNRTG	2 cut(s)	69, 1736
MspA1I	CMGCKG	4 cut(s)	91, 458, 848, 874
MspI	CCGG	5 cut(s)	59, 203, 253, 559, 916
MspR9I	CCNGG	5 cut(s)	254, 559, 917, 1229, 1533
MvaI	CCWGG	2 cut(s)	1229, 1533
MvnI	CGCG	4 cut(s)	133, 1216, 1829, 1831
MwoI	GCNNNNNNNGC	9 cut(s)	82, 223, 440, 452, 461, 527, 559, 854, 1244
NciI	CCSGG	3 cut(s)	254, 559, 917
NcoI	CCATGG	3 cut(s)	438, 678, 1710
NdeII	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
NlaIV	GGNNCC	2 cut(s)	201, 1210
NmeAIII	GCCGAG	1 cut(s)	180
NmuCI	GTSAC	1 cut(s)	1061
NruI	TCGCGA	1 cut(s)	1216
NsbI	TGCGCA	1 cut(s)	489
NsiI	ATGCAT	1 cut(s)	768
NspI	RCATGY	2 cut(s)	1129, 1334
PaeI	GCATGC	1 cut(s)	1334
PaeR7I	CTCGAG	1 cut(s)	227
PagI	TCATGA	1 cut(s)	1588
PasI	CCCWGGG	2 cut(s)	1228, 1532
PceI	AGGCCT	1 cut(s)	674
PciSI	GCTCTTC	2 cut(s)	116, 321
PdmI	GAANNNNTTC	2 cut(s)	381, 522
PfeI	GAWTC	2 cut(s)	144, 377
PinAI	ACCGGT	1 cut(s)	58
PshAI	GACNNNNGTC	1 cut(s)	1222
PshBI	ATTAAT	1 cut(s)	1170
Psp124BI	GAGCTC	5 cut(s)	128, 177, 219, 333, 549
Psp6I	CCWGG	2 cut(s)	1227, 1531
PspGI	CCWGG	2 cut(s)	1227, 1531
PspN4I	GGNNCC	2 cut(s)	201, 1210
PspOMI	GGGCCC	1 cut(s)	1208
PspPI	GGNCC	7 cut(s)	56, 250, 913, 925, 1208, 1209, 1761
PstNI	CAGNNNCTG	1 cut(s)	1228
PsuI	RGATCY	1 cut(s)	199
PvuII	CAGCTG	1 cut(s)	458
RruI	TCGCGA	1 cut(s)	1216
RsaI	GTAC	4 cut(s)	47, 1198, 1366, 1405
RsaNI	GTAC	4 cut(s)	46, 1197, 1365, 1404
RseI	CAYNNNNRTG	2 cut(s)	69, 1736
Rsr2I	CGGWCCG	1 cut(s)	56
RsrII	CGGWCCG	1 cut(s)	56
SacI	GAGCTC	5 cut(s)	128, 177, 219, 333, 549
SapI	GCTCTTC	2 cut(s)	116, 321
SaqAI	TTAA	6 cut(s)	408, 1134, 1170, 1256, 1325, 1841
Sau3AI	GATC	7 cut(s)	104, 199, 279, 601, 688, 1370, 1597
Sau96I	GGNCC	7 cut(s)	56, 250, 913, 925, 1208, 1209, 1761
ScrFI	CCNGG	5 cut(s)	254, 559, 917, 1229, 1533
SduI	GDGCHC	8 cut(s)	128, 177, 219, 333, 549, 1212, 1343, 1804
SfaNI	GCATC	4 cut(s)	753, 973, 990, 1612
SfcI	CTRYAG	1 cut(s)	957
Sfr274I	CTCGAG	1 cut(s)	227
SinI	GGWCC	3 cut(s)	56, 250, 913
SlaI	CTCGAG	1 cut(s)	227
SmiMI	CAYNNNNRTG	2 cut(s)	69, 1736
SmlI	CTYRAG	5 cut(s)	227, 290, 548, 635, 1142
SmoI	CTYRAG	5 cut(s)	227, 290, 548, 635, 1142
SphI	GCATGC	1 cut(s)	1334
Sse9I	AATT	5 cut(s)	405, 729, 750, 1253, 1480
SseBI	AGGCCT	1 cut(s)	674
SsiI	CCGC	9 cut(s)	83, 86, 89, 133, 811, 848, 874, 1768, 1846
SspMI	CTAG	1 cut(s)	386
SstI	GAGCTC	5 cut(s)	128, 177, 219, 333, 549
StuI	AGGCCT	1 cut(s)	674
StyD4I	CCNGG	5 cut(s)	252, 557, 915, 1227, 1531
StyI	CCWWGG	4 cut(s)	438, 663, 678, 1710
TaaI	ACNGT	7 cut(s)	50, 652, 808, 1166, 1183, 1403, 1510
TaiI	ACGT	1 cut(s)	1222
TaqI	TCGA	4 cut(s)	213, 228, 347, 1505
TasI	AATT	5 cut(s)	405, 729, 750, 1253, 1480
TatI	WGTACW	2 cut(s)	45, 1196
TauI	GCSGC	3 cut(s)	88, 91, 851
TfiI	GAWTC	2 cut(s)	144, 377
Tru1I	TTAA	6 cut(s)	408, 1134, 1170, 1256, 1325, 1841
Tru9I	TTAA	6 cut(s)	408, 1134, 1170, 1256, 1325, 1841
TscAI	CASTG	4 cut(s)	340, 1326, 1515, 1804
TseFI	GTSAC	1 cut(s)	1061
TseI	GCWGC	9 cut(s)	443, 455, 485, 626, 845, 1088, 1176, 1727, 1848
Tsp45I	GTSAC	1 cut(s)	1061
TspDTI	ATGAA	6 cut(s)	53, 249, 470, 731, 905, 1263
TspGWI	ACGGA	6 cut(s)	36, 56, 69, 487, 1042, 1532
TspRI	CASTG	4 cut(s)	340, 1326, 1515, 1804
VpaK11BI	GGWCC	3 cut(s)	56, 250, 913
VspI	ATTAAT	1 cut(s)	1170
XapI	RAATTY	3 cut(s)	729, 750, 1480
XbaI	TCTAGA	1 cut(s)	385
XceI	RCATGY	2 cut(s)	1129, 1334
XhoI	CTCGAG	1 cut(s)	227
XmnI	GAANNNNTTC	2 cut(s)	381, 522
XspI	CTAG	1 cut(s)	386
ZraI	GACGTC	1 cut(s)	1220
Zsp2I	ATGCAT	1 cut(s)	768

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.