Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009268

Physical Location & Seq

Genomic Coordinates Reverse (-)

22469519 .. 22471909

Gene Structure 2391 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1107300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1329 bp

ATGGTCAGGCTCTATCTCCTCTCTTCTTCCACTCACCTCCTTTCTTTCTGTAGAAAATACACTCTCCACACCCTGACCATACGAGCTCTTTCTTCCACCTCCACCGCCGCCACCATGTCCATAAGCCTCCAGTCCCACGCCTTCGCTGGCAATCCTCTGCTATCCAAGACCCCAAATCCCAGCGACCCGTTTTCACCATCCCAAGCCCTCGAGACCCTCAAGACCCAACTTTTGGAAGGGGCCCAATTGCCCTCTTCCCTCGGCTTCAAGGTTCTGCCCTTCAGAAAGGGCAGGCCTTTAGCCTCCTCCAGCGGTGGGGCCGGCGATTCCGCGCCAAATTGGCATCTGGGTTGGATCAGTTTGTCTGAGTGTAAAGGTTTCCTGGCCAATTGTGGGGTTCAGCTGAGTGCAGAGTCGCTGGTTTATCTGGGTTCTCGGCCCGAAGACGATGTCGTTTATTGGGCAATCGATGTTTCTGCTGAGAGTAAATTGGTTACTGAATTGGGTAGCAAGCAATTGTGTTTTGTTGAGCTGAGGACGCTTATGGTGGCTACTGATTGGGCTGATGCTCGAGCTATGGGGGAGTTGGCTATTGCTGGACATGCCAGGGCTTTGCTAGAATGGCATAGTATATCACAGTTTTGTGGACATTGTGGAGAAAAAACAGTCCCCAAGGAAGCTGGGAGGCGGAAGCAATGTTCTAGTGAGCAGTGCAGAAAGCGGGTCTACCCTCGTGTTGATCCGGTTGTCATAATGTTAGTCATTGATAGAGAGAATGACCGTGCTCTATTAAGTAGACAGTTTCGATTTGTACCTCGAATGTGGAGCTGCTTAGCTGGTTTTATAGAGCCAGGAGAAAGCTTAGAAGAAGCAGTGAGGAGAGAAACGTGGGAGGAGACCGGAATTGAAGTAGGAGAAGTTGTCTATCATAGCTCTCAGCCATGGCCTGTTGGACCAAGTAGCATGCCGTGCCAGTTGATGGTTGGGTTTTTTGCATATGCAAAGTCACTTGAAATAAATGTGGACAAGGAAGAGTTGGAAGATGCTCAGTGGCACAGTAGAGAAGATGTAAAGAGAGCTTTGACATTTGCTGAGTACAAGAAGGCACAGAGAACGGCGGCATCAAGGGTAGAACAGATGTGCAAAGGAGTAGAGAAAGAGCAGAGCTTGTCTGCCGACTTCAATGTTGAAAGTGGCGAACTCGCCTCCATGTTTATACCCGGGCCATTTGCAATTGCCCATCACCTTATCTCTTCATGGGCCTATCAGGATGCAACCGATGGTGCTGCAGCTCTAAAACAATCTAGCGGTTCTACTTCGAATTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00760 ko01100 ko04146 map00760 map01100 map04146

Pfam Domains

Protein Families

NUDIX NUDIX-like zf-NADH-PPase

Protein Analysis

443

Amino Acids

48.65

Weight (kDa)

6.16

Isoelectric Point (pI)

44.62

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NUDIX-like	PF09296	91 - 208	2.2e-06	NADH pyrophosphatase-like rudimentary NUDIX domain
Zn_ribbon_NUD	PF09297	212 - 243	8.8e-08	NADH pyrophosphatase zinc ribbon domain
NUDIX	PF00293	249 - 361	1.3e-19	NUDIX domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009268)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G20070
fragaria_vesca	FvH4_1g03600 FvH4_1g03600 FvH4_1g03600
malus_domestica	MD00G1107300.v1.1 MD02G1035600.v1.1 MD15G1175700.v1.1
prunus_persica	Prupe.7G239600_v2.0.a1
pyrus_communis	pycom02g02940 pycom15g15770 pycom15g15780
rosa_chinensis	RchiOBHm_Chr2g0089131
rosa_laevigata	RLG00000015994
rosa_multiflora	Rmu_sc0016750.1_g000004 Rmu_sc0029975.1_g000004
rosa_roxburghii	Rroxscaffold_2G00152220
rosa_rugosa	Rorug01G0487600
rosa_samantha	Rh2AG043200 Rh2BG042000 Rh2CG043200 Rh2DG042200
rosa_wichuraiana	Rw2G003440

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	232, 979
AccI	GTMKAC	2 cut(s)	726, 796
AccII	CGCG	1 cut(s)	332
AciI	CCGC	8 cut(s)	105, 108, 312, 330, 688, 721, 1118, 1308
AclWI	GGATC	2 cut(s)	362, 734
AcoI	YGGCCR	1 cut(s)	384
AcsI	RAATTY	1 cut(s)	1321
AcuI	CTGAAG	1 cut(s)	265
AfaI	GTAC	2 cut(s)	813, 1097
AfiI	CCNNNNNNNGG	4 cut(s)	232, 315, 393, 979
AgsI	TTSAA	5 cut(s)	268, 908, 1013, 1183, 1190
AjnI	CCWGG	3 cut(s)	381, 605, 850
AloI	GAACNNNNNNTCC	2 cut(s)	682, 714
Alw21I	GWGCWC	2 cut(s)	88, 787
Alw26I	GTCTC	2 cut(s)	206, 890
AlwI	GGATC	2 cut(s)	362, 734
Ama87I	CYCGRG	3 cut(s)	209, 570, 1220
AoxI	GGCC	8 cut(s)	240, 293, 318, 384, 437, 944, 1223, 1260
ApaI	GGGCCC	1 cut(s)	244
ApeKI	GCWGC	3 cut(s)	828, 1286, 1289
ApoI	RAATTY	1 cut(s)	1321
ArsI	GACNNNNNNTTYG	4 cut(s)	214, 246, 435, 467
AspLEI	GCGC	1 cut(s)	334
AspS9I	GGNCC	7 cut(s)	240, 241, 318, 438, 953, 1223, 1260
AsuC2I	CCSGG	2 cut(s)	1221, 1222
AsuHPI	GGTGA	3 cut(s)	26, 186, 1235
AsuII	TTCGAA	1 cut(s)	1319
AvaI	CYCGRG	3 cut(s)	209, 570, 1220
AvaII	GGWCC	1 cut(s)	953
BaeGI	GKGCMC	1 cut(s)	244
BalI	TGGCCA	1 cut(s)	386
BanII	GRGCYC	2 cut(s)	88, 244
BauI	CACGAG	1 cut(s)	732
BbsI	GAAGAC	1 cut(s)	450
Bbv12I	GWGCWC	2 cut(s)	88, 787
BbvCI	CCTCAGC	1 cut(s)	533
BbvI	GCAGC	3 cut(s)	815, 1273, 1301
BccI	CCATC	4 cut(s)	205, 973, 1248, 1274
BceAI	ACGGC	2 cut(s)	952, 1131
BcgI	CGANNNNNNTGC	4 cut(s)	458, 492, 1268, 1302
BciT130I	CCWGG	3 cut(s)	383, 607, 852
BcnI	CCSGG	2 cut(s)	1221, 1222
BcoDI	GTCTC	2 cut(s)	206, 890
BfaI	CTAG	3 cut(s)	617, 702, 1305
BfmI	CTRYAG	2 cut(s)	49, 1287
BglI	GCCNNNNNGGC	1 cut(s)	340
BisI	GCNGC	5 cut(s)	108, 829, 1119, 1287, 1290
BlpI	GCTNAGC	1 cut(s)	832
BlsI	GCNGC	5 cut(s)	109, 830, 1120, 1288, 1291
Bme1390I	CCNGG	5 cut(s)	383, 607, 852, 1221, 1222
Bme18I	GGWCC	1 cut(s)	953
BmeT110I	CYCGRG	3 cut(s)	209, 570, 1220
BmgT120I	GGNCC	7 cut(s)	240, 241, 318, 438, 953, 1223, 1260
BmiI	GGNNCC	3 cut(s)	241, 242, 319
BmrFI	CCNGG	5 cut(s)	383, 607, 852, 1221, 1222
BmsI	GCATC	5 cut(s)	352, 556, 1033, 1130, 1261
BpiI	GAAGAC	1 cut(s)	450
BpmI	CTGGAG	2 cut(s)	113, 292
Bpu10I	CCTNAGC	1 cut(s)	533
Bpu1102I	GCTNAGC	1 cut(s)	832
Bpu14I	TTCGAA	1 cut(s)	1319
BpuEI	CTTGAG	1 cut(s)	203
BpuMI	CCSGG	2 cut(s)	1221, 1222
Bsa29I	ATCGAT	1 cut(s)	468
BsaI	GGTCTC	2 cut(s)	206, 890
BsaJI	CCNNGG	5 cut(s)	259, 606, 672, 941, 1220
BsaWI	WCCGGW	2 cut(s)	742, 899
BsaXI	ACNNNNNCTCC	2 cut(s)	906, 936
Bsc4I	CCNNNNNNNGG	4 cut(s)	232, 315, 393, 979
Bse118I	RCCGGY	1 cut(s)	320
Bse1I	ACTGG	2 cut(s)	130, 973
Bse3DI	GCAATG	1 cut(s)	701
BseBI	CCWGG	3 cut(s)	383, 607, 852
BseCI	ATCGAT	1 cut(s)	468
BseDI	CCNNGG	5 cut(s)	259, 606, 672, 941, 1220
BseGI	GGATG	2 cut(s)	197, 1276
BseLI	CCNNNNNNNGG	4 cut(s)	232, 315, 393, 979
BseMI	GCAATG	1 cut(s)	701
BseMII	CTCAG	7 cut(s)	357, 395, 471, 524, 950, 1061, 1083
BseNI	ACTGG	2 cut(s)	130, 973
BseRI	GAGGAG	4 cut(s)	8, 295, 892, 908
BseSI	GKGCMC	1 cut(s)	244
BseXI	GCAGC	3 cut(s)	815, 1273, 1301
BseYI	CCCAGC	2 cut(s)	179, 680
BsgI	GTGCAG	2 cut(s)	429, 733
Bsh1236I	CGCG	1 cut(s)	332
BshFI	GGCC	8 cut(s)	242, 295, 320, 386, 439, 946, 1225, 1262
BshVI	ATCGAT	1 cut(s)	468
BsiHKAI	GWGCWC	2 cut(s)	88, 787
BsiHKCI	CYCGRG	3 cut(s)	209, 570, 1220
BsiSI	CCGG	4 cut(s)	321, 743, 900, 1221
BslFI	GGGAC	2 cut(s)	118, 653
BslI	CCNNNNNNNGG	4 cut(s)	232, 315, 393, 979
BsmAI	GTCTC	2 cut(s)	206, 890
BsmFI	GGGAC	2 cut(s)	118, 653
BsnI	GGCC	8 cut(s)	242, 295, 320, 386, 439, 946, 1225, 1262
Bso31I	GGTCTC	2 cut(s)	206, 890
BsoBI	CYCGRG	3 cut(s)	209, 570, 1220
Bsp119I	TTCGAA	1 cut(s)	1319
Bsp120I	GGGCCC	1 cut(s)	240
Bsp1286I	GDGCHC	3 cut(s)	88, 244, 787
Bsp143I	GATC	2 cut(s)	354, 739
Bsp1720I	GCTNAGC	1 cut(s)	832
Bsp19I	CCATGG	1 cut(s)	941
BspACI	CCGC	8 cut(s)	105, 108, 312, 330, 688, 721, 1118, 1308
BspANI	GGCC	8 cut(s)	242, 295, 320, 386, 439, 946, 1225, 1262
BspCNI	CTCAG	7 cut(s)	358, 396, 472, 525, 949, 1060, 1084
BspDI	ATCGAT	1 cut(s)	468
BspFNI	CGCG	1 cut(s)	332
BspLI	GGNNCC	3 cut(s)	241, 242, 319
BspMAI	CTGCAG	1 cut(s)	1291
BspPI	GGATC	2 cut(s)	362, 734
BspT104I	TTCGAA	1 cut(s)	1319
BspTNI	GGTCTC	2 cut(s)	206, 890
BsrDI	GCAATG	1 cut(s)	701
BsrFI	RCCGGY	1 cut(s)	320
BsrI	ACTGG	2 cut(s)	130, 973
BssAI	RCCGGY	1 cut(s)	320
BssECI	CCNNGG	5 cut(s)	259, 606, 672, 941, 1220
BssMI	GATC	2 cut(s)	354, 739
BssSI	CACGAG	1 cut(s)	732
BssT1I	CCWWGG	2 cut(s)	672, 941
Bst2BI	CACGAG	1 cut(s)	732
Bst2UI	CCWGG	3 cut(s)	383, 607, 852
Bst4CI	ACNGT	5 cut(s)	639, 667, 782, 801, 1058
Bst6I	CTCTTC	4 cut(s)	28, 259, 1026, 1258
BstAPI	GCANNNNNTGC	1 cut(s)	969
BstBI	TTCGAA	1 cut(s)	1319
BstC8I	GCNNGC	5 cut(s)	148, 293, 322, 512, 965
BstDEI	CTNAG	9 cut(s)	366, 404, 480, 533, 832, 862, 936, 1047, 1092
BstDSI	CCRYGG	1 cut(s)	941
BstF5I	GGATG	2 cut(s)	197, 1276
BstFNI	CGCG	1 cut(s)	332
BstHHI	GCGC	1 cut(s)	334
BstKTI	GATC	2 cut(s)	357, 742
BstMAI	GTCTC	2 cut(s)	206, 890
BstMBI	GATC	2 cut(s)	354, 739
BstMWI	GCNNNNNNNGC	5 cut(s)	340, 538, 602, 622, 969
BstNI	CCWGG	3 cut(s)	383, 607, 852
BstNSI	RCATGY	2 cut(s)	605, 967
BstSCI	CCNGG	5 cut(s)	381, 605, 850, 1219, 1220
BstSFI	CTRYAG	2 cut(s)	49, 1287
BstSLI	GKGCMC	1 cut(s)	244
BstUI	CGCG	1 cut(s)	332
BstV1I	GCAGC	3 cut(s)	815, 1273, 1301
BstV2I	GAAGAC	1 cut(s)	450
Bsu15I	ATCGAT	1 cut(s)	468
BsuRI	GGCC	8 cut(s)	242, 295, 320, 386, 439, 946, 1225, 1262
BsuTUI	ATCGAT	1 cut(s)	468
BtgI	CCRYGG	1 cut(s)	941
BtsCI	GGATG	2 cut(s)	197, 1276
BtsI	GCAGTG	2 cut(s)	716, 879
BtsIMutI	CAGTG	3 cut(s)	716, 879, 1055
Cac8I	GCNNGC	5 cut(s)	148, 293, 322, 512, 965
CfoI	GCGC	1 cut(s)	334
Cfr10I	RCCGGY	1 cut(s)	320
Cfr13I	GGNCC	7 cut(s)	240, 241, 318, 438, 953, 1223, 1260
Cfr9I	CCCGGG	1 cut(s)	1220
ClaI	ATCGAT	1 cut(s)	468
CseI	GACGC	1 cut(s)	547
Csp6I	GTAC	2 cut(s)	812, 1096
CviAII	CATG	6 cut(s)	115, 602, 942, 964, 1210, 1257
CviQI	GTAC	2 cut(s)	812, 1096
DdeI	CTNAG	9 cut(s)	366, 404, 480, 533, 832, 862, 936, 1047, 1092
DpnI	GATC	2 cut(s)	356, 741
DpnII	GATC	2 cut(s)	354, 739
EaeI	YGGCCR	1 cut(s)	384
Eam1104I	CTCTTC	4 cut(s)	28, 259, 1026, 1258
EarI	CTCTTC	4 cut(s)	28, 259, 1026, 1258
EciI	GGCGGA	1 cut(s)	703
Ecl136II	GAGCTC	1 cut(s)	86
Eco130I	CCWWGG	2 cut(s)	672, 941
Eco147I	AGGCCT	1 cut(s)	295
Eco24I	GRGCYC	2 cut(s)	88, 244
Eco31I	GGTCTC	2 cut(s)	206, 890
Eco47I	GGWCC	1 cut(s)	953
Eco53kI	GAGCTC	1 cut(s)	86
Eco57I	CTGAAG	1 cut(s)	265
Eco88I	CYCGRG	3 cut(s)	209, 570, 1220
EcoICRI	GAGCTC	1 cut(s)	86
EcoO109I	RGGNCCY	1 cut(s)	240
EcoRII	CCWGG	3 cut(s)	381, 605, 850
EcoT14I	CCWWGG	2 cut(s)	672, 941
EcoT38I	GRGCYC	2 cut(s)	88, 244
ErhI	CCWWGG	2 cut(s)	672, 941
FaeI	CATG	6 cut(s)	118, 605, 945, 967, 1213, 1260
FaqI	GGGAC	2 cut(s)	118, 653
FatI	CATG	6 cut(s)	114, 601, 941, 963, 1209, 1256
FauI	CCCGC	1 cut(s)	714
FauNDI	CATATG	1 cut(s)	997
FblI	GTMKAC	2 cut(s)	726, 796
Fnu4HI	GCNGC	5 cut(s)	108, 829, 1119, 1287, 1290
FokI	GGATG	2 cut(s)	184, 1283
FriOI	GRGCYC	2 cut(s)	88, 244
Fsp4HI	GCNGC	5 cut(s)	108, 829, 1119, 1287, 1290
FspBI	CTAG	3 cut(s)	617, 702, 1305
GlaI	GCGC	1 cut(s)	333
GluI	GCNGC	5 cut(s)	108, 829, 1119, 1287, 1290
GsaI	CCCAGC	2 cut(s)	183, 684
GsuI	CTGGAG	2 cut(s)	113, 292
HaeIII	GGCC	8 cut(s)	242, 295, 320, 386, 439, 946, 1225, 1262
HapII	CCGG	4 cut(s)	321, 743, 900, 1221
HgaI	GACGC	1 cut(s)	547
HhaI	GCGC	1 cut(s)	334
Hin1II	CATG	6 cut(s)	118, 605, 945, 967, 1213, 1260
Hin6I	GCGC	1 cut(s)	332
HinP1I	GCGC	1 cut(s)	332
HindIII	AAGCTT	1 cut(s)	859
HinfI	GANTC	2 cut(s)	326, 413
HpaII	CCGG	4 cut(s)	321, 743, 900, 1221
HphI	GGTGA	3 cut(s)	26, 186, 1235
Hpy166II	GTNNAC	4 cut(s)	647, 727, 797, 1024
Hpy188I	TCNGA	2 cut(s)	284, 367
Hpy188III	TCNNGA	3 cut(s)	211, 220, 1268
Hpy8I	GTNNAC	4 cut(s)	647, 727, 797, 1024
HpyAV	CCTTC	4 cut(s)	151, 230, 289, 1096
HpyCH4III	ACNGT	5 cut(s)	639, 667, 782, 801, 1058
HpyCH4IV	ACGT	1 cut(s)	887
HpyCH4V	TGCA	8 cut(s)	410, 714, 995, 1001, 1143, 1232, 1274, 1289
HpyF10VI	GCNNNNNNNGC	5 cut(s)	340, 538, 602, 622, 969
HpyF3I	CTNAG	9 cut(s)	366, 404, 480, 533, 832, 862, 936, 1047, 1092
HpySE526I	ACGT	1 cut(s)	887
Hsp92II	CATG	6 cut(s)	118, 605, 945, 967, 1213, 1260
HspAI	GCGC	1 cut(s)	332
KroI	GCCGGC	1 cut(s)	320
KroNI	GCCGGC	1 cut(s)	322
Kzo9I	GATC	2 cut(s)	354, 739
LmnI	GCTCC	1 cut(s)	825
Lsp1109I	GCAGC	3 cut(s)	815, 1273, 1301
LweI	GCATC	5 cut(s)	352, 556, 1033, 1130, 1261
MaeI	CTAG	3 cut(s)	617, 702, 1305
MaeII	ACGT	1 cut(s)	887
MaeIII	GTNAC	2 cut(s)	493, 1005
MalI	GATC	2 cut(s)	356, 741
MboI	GATC	2 cut(s)	354, 739
MfeI	CAATTG	4 cut(s)	245, 388, 515, 1233
MhlI	GDGCHC	3 cut(s)	88, 244, 787
MlsI	TGGCCA	1 cut(s)	386
MluCI	AATT	9 cut(s)	245, 337, 388, 488, 500, 515, 903, 1233, 1321
MluNI	TGGCCA	1 cut(s)	386
MlyI	GAGTC	1 cut(s)	422
MmeI	TCCRAC	3 cut(s)	332, 931, 1017
Mox20I	TGGCCA	1 cut(s)	386
MroNI	GCCGGC	1 cut(s)	320
MscI	TGGCCA	1 cut(s)	386
MseI	TTAA	1 cut(s)	791
Msp20I	TGGCCA	1 cut(s)	386
MspA1I	CMGCKG	2 cut(s)	312, 403
MspI	CCGG	4 cut(s)	321, 743, 900, 1221
MspR9I	CCNGG	5 cut(s)	383, 607, 852, 1221, 1222
MunI	CAATTG	4 cut(s)	245, 388, 515, 1233
MvaI	CCWGG	3 cut(s)	383, 607, 852
MvnI	CGCG	1 cut(s)	332
MwoI	GCNNNNNNNGC	5 cut(s)	340, 538, 602, 622, 969
NaeI	GCCGGC	1 cut(s)	322
NciI	CCSGG	2 cut(s)	1221, 1222
NcoI	CCATGG	1 cut(s)	941
NdeI	CATATG	1 cut(s)	997
NdeII	GATC	2 cut(s)	354, 739
NgoMIV	GCCGGC	1 cut(s)	320
NlaIII	CATG	6 cut(s)	118, 605, 945, 967, 1213, 1260
NlaIV	GGNNCC	3 cut(s)	241, 242, 319
NmeAIII	GCCGAG	2 cut(s)	240, 415
NmuCI	GTSAC	1 cut(s)	1005
NspI	RCATGY	2 cut(s)	605, 967
NspV	TTCGAA	1 cut(s)	1319
PaeI	GCATGC	1 cut(s)	967
PaeR7I	CTCGAG	2 cut(s)	209, 570
PceI	AGGCCT	1 cut(s)	295
PdiI	GCCGGC	1 cut(s)	322
PfeI	GAWTC	1 cut(s)	326
PflFI	GACNNNGTC	1 cut(s)	449
PflMI	CCANNNNNTGG	2 cut(s)	232, 979
PkrI	GCNGC	5 cut(s)	109, 830, 1120, 1288, 1291
PleI	GAGTC	1 cut(s)	421
PpsI	GAGTC	1 cut(s)	421
Psp124BI	GAGCTC	1 cut(s)	88
Psp6I	CCWGG	3 cut(s)	381, 605, 850
PspFI	CCCAGC	2 cut(s)	179, 680
PspGI	CCWGG	3 cut(s)	381, 605, 850
PspN4I	GGNNCC	3 cut(s)	241, 242, 319
PspOMI	GGGCCC	1 cut(s)	240
PspPI	GGNCC	7 cut(s)	240, 241, 318, 438, 953, 1223, 1260
PspXI	VCTCGAGB	1 cut(s)	570
PstI	CTGCAG	1 cut(s)	1291
PsyI	GACNNNGTC	1 cut(s)	449
PvuII	CAGCTG	1 cut(s)	403
RsaI	GTAC	2 cut(s)	813, 1097
RsaNI	GTAC	2 cut(s)	812, 1096
SacI	GAGCTC	1 cut(s)	88
SaqAI	TTAA	1 cut(s)	791
SatI	GCNGC	5 cut(s)	108, 829, 1119, 1287, 1290
Sau3AI	GATC	2 cut(s)	354, 739
Sau96I	GGNCC	7 cut(s)	240, 241, 318, 438, 953, 1223, 1260
SchI	GAGTC	1 cut(s)	422
ScrFI	CCNGG	5 cut(s)	383, 607, 852, 1221, 1222
SduI	GDGCHC	3 cut(s)	88, 244, 787
SfaNI	GCATC	5 cut(s)	352, 556, 1033, 1130, 1261
SfcI	CTRYAG	2 cut(s)	49, 1287
Sfr274I	CTCGAG	2 cut(s)	209, 570
SfuI	TTCGAA	1 cut(s)	1319
SinI	GGWCC	1 cut(s)	953
SlaI	CTCGAG	2 cut(s)	209, 570
SmaI	CCCGGG	1 cut(s)	1222
SmlI	CTYRAG	3 cut(s)	209, 218, 570
SmoI	CTYRAG	3 cut(s)	209, 218, 570
SphI	GCATGC	1 cut(s)	967
Sse9I	AATT	9 cut(s)	245, 337, 388, 488, 500, 515, 903, 1233, 1321
SseBI	AGGCCT	1 cut(s)	295
SsiI	CCGC	8 cut(s)	105, 108, 312, 330, 688, 721, 1118, 1308
SspMI	CTAG	3 cut(s)	617, 702, 1305
SstI	GAGCTC	1 cut(s)	88
StuI	AGGCCT	1 cut(s)	295
StyD4I	CCNGG	5 cut(s)	381, 605, 850, 1219, 1220
StyI	CCWWGG	2 cut(s)	672, 941
TaaI	ACNGT	5 cut(s)	639, 667, 782, 801, 1058
TaiI	ACGT	1 cut(s)	890
TaqI	TCGA	6 cut(s)	210, 468, 571, 805, 817, 1319
TasI	AATT	9 cut(s)	245, 337, 388, 488, 500, 515, 903, 1233, 1321
TatI	WGTACW	1 cut(s)	1095
TauI	GCSGC	2 cut(s)	110, 1121
TfiI	GAWTC	1 cut(s)	326
Tru1I	TTAA	1 cut(s)	791
Tru9I	TTAA	1 cut(s)	791
TscAI	CASTG	3 cut(s)	716, 879, 1055
TseFI	GTSAC	1 cut(s)	1005
TseI	GCWGC	3 cut(s)	828, 1286, 1289
Tsp45I	GTSAC	1 cut(s)	1005
TspDTI	ATGAA	1 cut(s)	1245
TspMI	CCCGGG	1 cut(s)	1220
TspRI	CASTG	3 cut(s)	716, 879, 1055
Tth111I	GACNNNGTC	1 cut(s)	449
Van91I	CCANNNNNTGG	2 cut(s)	232, 979
VpaK11BI	GGWCC	1 cut(s)	953
XapI	RAATTY	1 cut(s)	1321
XceI	RCATGY	2 cut(s)	605, 967
XcmI	CCANNNNNNNNNTGG	2 cut(s)	143, 980
XhoI	CTCGAG	2 cut(s)	209, 570
XmaI	CCCGGG	1 cut(s)	1220
XmiI	GTMKAC	2 cut(s)	726, 796
XspI	CTAG	3 cut(s)	617, 702, 1305

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.