MD00G1118200.v1.1

Belongs to the DHHC palmitoyltransferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007585
Physical Location & Seq
Forward (+)
25240001 .. 25243574
3574 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1118200.v1.1.491

Sequence Viewer

Length: 1398 bp
ATGAGCAGGAAAAAGAGCCAAGTCTGGGATGCTCCCTCCGACGACACAGCCGCGGCGGCCCCTCCTCCTCCTGAAAGAGAAAGGCAATACCTTGTGTGGCAGGGTAACAATAAATTTATGTGCGGTGGAAGAATTGTGTTTGGTCATGACGCCGCATCGCTGTTTCTAACAAGCTTTTTGATTGGATGTCCTGCACTAACATTCTGCATAAGAATGCTTGTAATGATGAGACGACAAGATGGACATTACGACTATCCCGTACTAATTGGGGGAATAATCCTCACTGTTTTGGATTTCATTTTTCTATACTTGACATCCGGTAGAGATCCTGGTATTATTCCGCGGAACGCACGACCACCAGAATCAAGCGAGGCATATGACGGTCCTACCCCTTCAATGGAGTGGGTCAATAACAAATCCTCCAGTTTGAGAATACCCCGAATAAAGGATGTAACTGTCAATGGCCACACAGTGAAGGTCAAATTTTGTGACACTTGTTTGCTGTATCGTCCACCACGTGCCTCTCATTGTTCCATCTGCAATAACTGTGTTCAGAAATTTGATCACCATTGTCCATGGGTGGGCCAGTGTATTGGATTACGTAACTATCCGTTTTTCATTTGTTTTATTTCGTCCTCAACGTTGCTATGCATATATGTGTTTGTGTTTTCGTGGATCAACCTTGTTCGACAAGGAGGCAATCTATGGAGGGCCATGGCGCGTGACATCGTATCAGTTATTCTCATAGTGTACTGCTTTGTAGCAGTCTGGTTTGTTGGTGGCCTTACAGTTTTCCATATCTATCTGATTTGCACAAACCAGACAACATATGAGAATTTCCGATACCGTTACGATAAGAACGAAAACCCCTACACAAAGGGACTACCGGGAAACCTGAAAGAAGTGTTCTGCTCAAGGATTCCTCCCTCGATGGTTAACTTCAGAGAATGGGTTTCGGTTAAGGACATCAACGACGTCGACATAGGAATGGCATCCATCGCTTCAGAGAGCAATAGAGGCTTCATCGCCTCCAAAGAGAAATTTGACATAGAAAAGGGAAATAAGTTTGGAAAGGATCATGGCGGCAATATCATTGTTCCAAGTATTCTGCAGAAATTGGACTACAGTGGCATTGATGATGGTAACATGAAGAAGAACAGGAACAATAATAATTTGAGTGGCGGTGGCTTTAATGATAATAATCTAAAGAAGGGGACGGCAGCAGAAGACGGTGATGTTGGACCTGCTGCTGCTGGATTCATTTTGTTTCCTAATAACAATACTGGTCAAGAAGAAAAAACAAATTTAGATGATCATCGGGGGATGAAGAGTAGTTCCGACCGAGGATCAGTTAATTCATCTACTCATGGTAATGAAAAGAGGACTATTCATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04391 map04391
Pfam Domains
Protein Families
DHHC

Protein Analysis

466

Amino Acids

52.11

Weight (kDa)

8.71

Isoelectric Point (pI)

34.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHHC PF01529 160 - 281 1.5e-37 DHHC palmitoyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 978
Acc36I ACCTGC 1 cut(s) 1252
AccI GTMKAC 1 cut(s) 978
AccII CGCG 3 cut(s) 53, 343, 721
AciI CCGC 9 cut(s) 51, 53, 56, 123, 153, 341, 343, 1083, 1182
AclI AACGTT 1 cut(s) 641
AclWI GGATC 4 cut(s) 320, 683, 1083, 1354
AcoI YGGCCR 1 cut(s) 463
AcsI RAATTY 6 cut(s) 113, 482, 557, 835, 1040, 1303
AcuI CTGAAG 2 cut(s) 925, 987
AcvI CACGTG 1 cut(s) 518
AcyI GRCGYC 2 cut(s) 150, 975
AdeI CACNNNGTG 2 cut(s) 472, 518
AfaI GTAC 2 cut(s) 261, 752
AfiI CCNNNNNNNGG 4 cut(s) 25, 397, 445, 581
AgsI TTSAA 1 cut(s) 396
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 1 cut(s) 174
AluI AGCT 1 cut(s) 174
Alw26I GTCTC 1 cut(s) 223
AlwI GGATC 4 cut(s) 320, 683, 1083, 1354
AoxI GGCC 5 cut(s) 57, 463, 583, 711, 781
ApeKI GCWGC 3 cut(s) 1220, 1247, 1250
ApoI RAATTY 6 cut(s) 113, 482, 557, 835, 1040, 1303
AspLEI GCGC 1 cut(s) 721
AspS9I GGNCC 5 cut(s) 58, 383, 583, 711, 1241
AsuC2I CCSGG 1 cut(s) 888
AsuHPI GGTGA 2 cut(s) 557, 1244
AvaII GGWCC 2 cut(s) 383, 1241
BalI TGGCCA 1 cut(s) 465
BbrPI CACGTG 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 1233
BbvI GCAGC 3 cut(s) 1232, 1234, 1237
BccI CCATC 5 cut(s) 233, 542, 925, 1004, 1133
BceAI ACGGC 1 cut(s) 1233
BciT130I CCWGG 1 cut(s) 330
BclI TGATCA 2 cut(s) 562, 1312
BcnI CCSGG 1 cut(s) 888
BcoDI GTCTC 1 cut(s) 223
BfmI CTRYAG 2 cut(s) 1109, 1123
BfuAI ACCTGC 1 cut(s) 1252
BglI GCCNNNNNGGC 1 cut(s) 56
BisI GCNGC 8 cut(s) 51, 54, 57, 153, 1084, 1221, 1248, 1251
BlsI GCNGC 8 cut(s) 52, 55, 58, 154, 1085, 1222, 1249, 1252
Bme1390I CCNGG 2 cut(s) 330, 888
Bme18I GGWCC 2 cut(s) 383, 1241
BmgT120I GGNCC 5 cut(s) 58, 383, 583, 711, 1241
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 2 cut(s) 330, 888
BmsI GCATC 3 cut(s) 19, 164, 1001
BpiI GAAGAC 1 cut(s) 1233
BpmI CTGGAG 1 cut(s) 406
BpuEI CTTGAG 1 cut(s) 898
BpuMI CCSGG 1 cut(s) 888
BsaAI YACGTR 2 cut(s) 518, 602
BsaBI GATNNNNATC 2 cut(s) 1200, 1314
BsaHI GRCGYC 2 cut(s) 150, 975
BsaJI CCNNGG 5 cut(s) 51, 341, 575, 714, 1342
BsaWI WCCGGW 1 cut(s) 317
BsaXI ACNNNNNCTCC 2 cut(s) 404, 434
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 397, 445, 581
Bse1I ACTGG 3 cut(s) 423, 586, 1288
Bse8I GATNNNNATC 2 cut(s) 1200, 1314
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 5 cut(s) 51, 341, 575, 714, 1342
BseGI GGATG 6 cut(s) 34, 191, 314, 454, 992, 1329
BseJI GATNNNNATC 2 cut(s) 1200, 1314
BseLI CCNNNNNNNGG 4 cut(s) 25, 397, 445, 581
BseNI ACTGG 3 cut(s) 423, 586, 1288
BseRI GAGGAG 2 cut(s) 54, 57
BseXI GCAGC 3 cut(s) 1232, 1234, 1237
BsgI GTGCAG 1 cut(s) 177
Bsh1236I CGCG 3 cut(s) 53, 343, 721
Bsh1285I CGRYCG 1 cut(s) 1342
BshFI GGCC 5 cut(s) 59, 465, 585, 713, 783
BsiEI CGRYCG 1 cut(s) 1342
BsiSI CCGG 2 cut(s) 318, 887
BslFI GGGAC 2 cut(s) 894, 1228
BslI CCNNNNNNNGG 4 cut(s) 25, 397, 445, 581
BsmAI GTCTC 1 cut(s) 223
BsmBI CGTCTC 1 cut(s) 223
BsmFI GGGAC 2 cut(s) 894, 1228
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 5 cut(s) 59, 465, 585, 713, 783
Bsp143I GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
Bsp19I CCATGG 2 cut(s) 575, 714
BspACI CCGC 9 cut(s) 51, 53, 56, 123, 153, 341, 343, 1083, 1182
BspANI GGCC 5 cut(s) 59, 465, 585, 713, 783
BspFNI CGCG 3 cut(s) 53, 343, 721
BspHI TCATGA 1 cut(s) 145
BspLI GGNNCC 1 cut(s) 60
BspMAI CTGCAG 1 cut(s) 1113
BspMI ACCTGC 1 cut(s) 1252
BspPI GGATC 4 cut(s) 320, 683, 1083, 1354
BsrI ACTGG 3 cut(s) 423, 586, 1288
BssECI CCNNGG 5 cut(s) 51, 341, 575, 714, 1342
BssMI GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
BssNI GRCGYC 2 cut(s) 150, 975
BssT1I CCWWGG 2 cut(s) 575, 714
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 9 cut(s) 286, 383, 457, 472, 548, 790, 848, 1127, 1232
Bst6I CTCTTC 1 cut(s) 1322
BstACI GRCGYC 2 cut(s) 150, 975
BstBAI YACGTR 2 cut(s) 518, 602
BstDSI CCRYGG 4 cut(s) 51, 341, 575, 714
BstF5I GGATG 6 cut(s) 34, 191, 314, 454, 992, 1329
BstFNI CGCG 3 cut(s) 53, 343, 721
BstHHI GCGC 1 cut(s) 721
BstKTI GATC 6 cut(s) 328, 565, 678, 1078, 1315, 1349
BstMAI GTCTC 1 cut(s) 223
BstMBI GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
BstMCI CGRYCG 1 cut(s) 1342
BstMWI GCNNNNNNNGC 3 cut(s) 56, 998, 1017
BstNI CCWGG 1 cut(s) 330
BstSCI CCNGG 2 cut(s) 328, 886
BstSFI CTRYAG 2 cut(s) 1109, 1123
BstSNI TACGTA 1 cut(s) 602
BstUI CGCG 3 cut(s) 53, 343, 721
BstV1I GCAGC 3 cut(s) 1232, 1234, 1237
BstV2I GAAGAC 1 cut(s) 1233
BstX2I RGATCY 1 cut(s) 325
BstXI CCANNNNNNTGG 1 cut(s) 593
BstYI RGATCY 1 cut(s) 325
BsuRI GGCC 5 cut(s) 59, 465, 585, 713, 783
BtgI CCRYGG 4 cut(s) 51, 341, 575, 714
BtgZI GCGATG 3 cut(s) 141, 982, 1009
BtsCI GGATG 6 cut(s) 34, 191, 314, 454, 992, 1329
BtsIMutI CAGTG 4 cut(s) 282, 477, 593, 1132
BveI ACCTGC 1 cut(s) 1252
CciI TCATGA 1 cut(s) 145
CfoI GCGC 1 cut(s) 721
Cfr13I GGNCC 5 cut(s) 58, 383, 583, 711, 1241
Cfr42I CCGCGG 2 cut(s) 54, 344
CseI GACGC 1 cut(s) 158
Csp6I GTAC 2 cut(s) 260, 751
CviAII CATG 6 cut(s) 146, 576, 715, 1079, 1147, 1367
CviQI GTAC 2 cut(s) 260, 751
DpnI GATC 6 cut(s) 327, 564, 677, 1077, 1314, 1348
DpnII GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
DraIII CACNNNGTG 2 cut(s) 472, 518
EaeI YGGCCR 1 cut(s) 463
Eam1104I CTCTTC 1 cut(s) 1322
EarI CTCTTC 1 cut(s) 1322
Eco105I TACGTA 1 cut(s) 602
Eco130I CCWWGG 2 cut(s) 575, 714
Eco47I GGWCC 2 cut(s) 383, 1241
Eco57I CTGAAG 2 cut(s) 925, 987
Eco72I CACGTG 1 cut(s) 518
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 2 cut(s) 575, 714
EcoT22I ATGCAT 1 cut(s) 653
ErhI CCWWGG 2 cut(s) 575, 714
Esp3I CGTCTC 1 cut(s) 223
FaeI CATG 6 cut(s) 149, 579, 718, 1082, 1150, 1370
FaqI GGGAC 2 cut(s) 894, 1228
FatI CATG 6 cut(s) 145, 575, 714, 1078, 1146, 1366
FauNDI CATATG 2 cut(s) 376, 829
FbaI TGATCA 2 cut(s) 562, 1312
FblI GTMKAC 1 cut(s) 978
Fnu4HI GCNGC 8 cut(s) 51, 54, 57, 153, 1084, 1221, 1248, 1251
FokI GGATG 6 cut(s) 41, 198, 301, 461, 979, 1336
Fsp4HI GCNGC 8 cut(s) 51, 54, 57, 153, 1084, 1221, 1248, 1251
GlaI GCGC 1 cut(s) 720
GluI GCNGC 8 cut(s) 51, 54, 57, 153, 1084, 1221, 1248, 1251
GsuI CTGGAG 1 cut(s) 406
HaeIII GGCC 5 cut(s) 59, 465, 585, 713, 783
HapII CCGG 2 cut(s) 318, 887
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 721
Hin1I GRCGYC 2 cut(s) 150, 975
Hin1II CATG 6 cut(s) 149, 579, 718, 1082, 1150, 1370
Hin6I GCGC 1 cut(s) 719
HinP1I GCGC 1 cut(s) 719
HincII GTYRAC 2 cut(s) 937, 979
HindII GTYRAC 2 cut(s) 937, 979
HindIII AAGCTT 1 cut(s) 172
HinfI GANTC 3 cut(s) 362, 919, 1257
HpaI GTTAAC 1 cut(s) 937
HpaII CCGG 2 cut(s) 318, 887
HphI GGTGA 2 cut(s) 557, 1244
Hpy166II GTNNAC 4 cut(s) 512, 751, 937, 979
Hpy188I TCNGA 7 cut(s) 40, 555, 807, 842, 944, 1006, 1339
Hpy188III TCNNGA 3 cut(s) 71, 146, 1289
Hpy8I GTNNAC 4 cut(s) 512, 751, 937, 979
Hpy99I CGWCG 3 cut(s) 44, 977, 980
HpyAV CCTTC 3 cut(s) 402, 469, 1204
HpyCH4III ACNGT 9 cut(s) 286, 383, 457, 472, 548, 790, 848, 1127, 1232
HpyCH4IV ACGT 4 cut(s) 517, 601, 641, 975
HpyCH4V TGCA 6 cut(s) 194, 207, 540, 651, 813, 1111
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 998, 1017
HpySE526I ACGT 4 cut(s) 517, 601, 641, 975
Hsp92I GRCGYC 2 cut(s) 150, 975
Hsp92II CATG 6 cut(s) 149, 579, 718, 1082, 1150, 1370
HspAI GCGC 1 cut(s) 719
Ksp22I TGATCA 2 cut(s) 562, 1312
KspAI GTTAAC 1 cut(s) 937
KspI CCGCGG 2 cut(s) 54, 344
Kzo9I GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
LmnI GCTCC 1 cut(s) 37
Lsp1109I GCAGC 3 cut(s) 1232, 1234, 1237
LweI GCATC 3 cut(s) 19, 164, 1001
MaeII ACGT 4 cut(s) 517, 601, 641, 975
MaeIII GTNAC 7 cut(s) 104, 451, 488, 602, 722, 848, 1142
MalI GATC 6 cut(s) 327, 564, 677, 1077, 1314, 1348
MboI GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
MboII GAAGA 6 cut(s) 141, 1162, 1165, 1238, 1304, 1339
MflI RGATCY 1 cut(s) 325
MlsI TGGCCA 1 cut(s) 465
MluNI TGGCCA 1 cut(s) 465
MmeI TCCRAC 3 cut(s) 63, 1219, 1362
Mox20I TGGCCA 1 cut(s) 465
Mph1103I ATGCAT 1 cut(s) 653
MscI TGGCCA 1 cut(s) 465
MseI TTAA 4 cut(s) 936, 960, 1191, 1353
MslI CAYNNNNRTG 4 cut(s) 212, 656, 986, 1371
Msp20I TGGCCA 1 cut(s) 465
MspA1I CMGCKG 2 cut(s) 53, 343
MspI CCGG 2 cut(s) 318, 887
MspR9I CCNGG 2 cut(s) 330, 888
Mva1269I GAATGC 1 cut(s) 219
MvaI CCWGG 1 cut(s) 330
MvnI CGCG 3 cut(s) 53, 343, 721
MwoI GCNNNNNNNGC 3 cut(s) 56, 998, 1017
NciI CCSGG 1 cut(s) 888
NcoI CCATGG 2 cut(s) 575, 714
NdeI CATATG 2 cut(s) 376, 829
NdeII GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
NlaIII CATG 6 cut(s) 149, 579, 718, 1082, 1150, 1370
NlaIV GGNNCC 1 cut(s) 60
NmuCI GTSAC 2 cut(s) 488, 722
NsiI ATGCAT 1 cut(s) 653
PagI TCATGA 1 cut(s) 145
PcsI WCGNNNNNNNCGW 4 cut(s) 255, 514, 638, 858
PctI GAATGC 1 cut(s) 219
PfeI GAWTC 3 cut(s) 362, 919, 1257
PkrI GCNGC 8 cut(s) 52, 55, 58, 154, 1085, 1222, 1249, 1252
PmaCI CACGTG 1 cut(s) 518
PmlI CACGTG 1 cut(s) 518
Ppu21I YACGTR 2 cut(s) 518, 602
Psp1406I AACGTT 1 cut(s) 641
Psp6I CCWGG 1 cut(s) 328
PspCI CACGTG 1 cut(s) 518
PspGI CCWGG 1 cut(s) 328
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 5 cut(s) 58, 383, 583, 711, 1241
PstI CTGCAG 1 cut(s) 1113
PsuI RGATCY 1 cut(s) 325
RsaI GTAC 2 cut(s) 261, 752
RsaNI GTAC 2 cut(s) 260, 751
RseI CAYNNNNRTG 4 cut(s) 212, 656, 986, 1371
SacII CCGCGG 2 cut(s) 54, 344
SalI GTCGAC 1 cut(s) 977
SaqAI TTAA 4 cut(s) 936, 960, 1191, 1353
SatI GCNGC 8 cut(s) 51, 54, 57, 153, 1084, 1221, 1248, 1251
Sau3AI GATC 6 cut(s) 325, 562, 675, 1075, 1312, 1346
Sau96I GGNCC 5 cut(s) 58, 383, 583, 711, 1241
ScrFI CCNGG 2 cut(s) 330, 888
SfaNI GCATC 3 cut(s) 19, 164, 1001
SfcI CTRYAG 2 cut(s) 1109, 1123
Sfr303I CCGCGG 2 cut(s) 54, 344
SgrBI CCGCGG 2 cut(s) 54, 344
SinI GGWCC 2 cut(s) 383, 1241
SmiMI CAYNNNNRTG 4 cut(s) 212, 656, 986, 1371
SmlI CTYRAG 1 cut(s) 913
SmoI CTYRAG 1 cut(s) 913
SnaBI TACGTA 1 cut(s) 602
SsiI CCGC 9 cut(s) 51, 53, 56, 123, 153, 341, 343, 1083, 1182
StyD4I CCNGG 2 cut(s) 328, 886
StyI CCWWGG 2 cut(s) 575, 714
TaaI ACNGT 9 cut(s) 286, 383, 457, 472, 548, 790, 848, 1127, 1232
TaiI ACGT 4 cut(s) 520, 604, 644, 978
TaqI TCGA 3 cut(s) 688, 929, 978
TaqII GACCGA 1 cut(s) 1356
TatI WGTACW 1 cut(s) 750
TauI GCSGC 5 cut(s) 53, 56, 59, 155, 1086
TfiI GAWTC 3 cut(s) 362, 919, 1257
Tru1I TTAA 4 cut(s) 936, 960, 1191, 1353
Tru9I TTAA 4 cut(s) 936, 960, 1191, 1353
TscAI CASTG 4 cut(s) 289, 477, 593, 1132
TseFI GTSAC 2 cut(s) 488, 722
TseI GCWGC 3 cut(s) 1220, 1247, 1250
Tsp45I GTSAC 2 cut(s) 488, 722
TspDTI ATGAA 9 cut(s) 286, 607, 1012, 1163, 1249, 1340, 1347, 1379, 1389
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 4 cut(s) 289, 477, 593, 1132
VpaK11BI GGWCC 2 cut(s) 383, 1241
XapI RAATTY 6 cut(s) 113, 482, 557, 835, 1040, 1303
XmiI GTMKAC 1 cut(s) 978
ZraI GACGTC 1 cut(s) 976
Zsp2I ATGCAT 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.