Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0011531

Physical Location & Seq

Genomic Coordinates Forward (+)

31240990 .. 31242443

Gene Structure 1454 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1143600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 837 bp

ATGGATGCTCATACCCTTAACAAGCAAAAGGTTGTAGACAGTGTTCCAGAAGACTTGCAACGTTTGAGGAAAGAGAATGAAACCCTAAGATTCCTGCTTCAAGTTATGACCGCCAAGTGCTCAACACTTGAGCAGCAGCAACTGCGCCATATGCATATCCAACAACAACCGTGTGATCATCGACATATCATGACCACGAATATCAACAGTGCGATTATGAGCAAGAGACAGAGAACTGATGAGCATCGCTATCAGTTTCCGGCATCCCACGATAAGACATCACAGTTCCTAGTGAAAACCGATTCTAAAGACAACAGCCTTATTGTGAAAGATGGATATCAATGGAGGAAATATGGACAGAAGGTCACCAAAGACAACCCTTCATCCCCTCGAGCTTATTTCAGGTGTTCCTTCGCTCCTCGATGCCCTGTCAAAAAGAAGGTACAGAGGTGCATAGAGGATGATTCCATCCTCGTGGCGACATACGAAGGAGAGCACAACCACGACGCCATCAATGATTCTGCACTATTTGGACAATTTTCATGTTCACATAAGAACATAATTGTGCCCGTTAATTTTCCTTCAAGTGGTATCGGGACTAATAATGACGTGATTAATATAAGTCCCTCTCCGCCGGAGCCTGTAACGCTCGATCTCAGTCTCTCGGGATCCAGTAATCAAGAAAATGGAGGATTGGGAAGCCCTCAAAGCATGGTTAGCTCCAGAAGAGTTGAGGATTACGTTGCTTCGTTAACCAAGGATCCTGATTTCACACAAACTTTAGTTGCTGCAGTTGCACGTTCGATTGCAAGACCTGCAAATAGACCAAATTCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002237 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

279

Amino Acids

31.28

Weight (kDa)

8.85

Isoelectric Point (pI)

65.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	110 - 169	1.9e-23	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011531)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41070 FvH4_2g41070 FvH4_2g41070
malus_domestica	MD00G1143600.v1.1 MD15G1039600.v1.1
prunus_persica	Prupe.1G393100_v2.0.a1
pyrus_communis	pycom08g04720 pycom08g04730 pycom08g04740 pycom15g03700
rosa_chinensis	RchiOBHm_Chr6g0299481
rosa_laevigata	RLG00000011428
rosa_multiflora	Rmu_sc0001184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00168640
rosa_rugosa	Rorug06G0289100
rosa_samantha	Rh6AG400400 Rh6BG409100 Rh6CG414600 Rh6DG401500
rosa_wichuraiana	Rw6G035050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	823
AccI	GTMKAC	1 cut(s)	36
AciI	CCGC	2 cut(s)	111, 632
AclI	AACGTT	1 cut(s)	61
AclWI	GGATC	4 cut(s)	663, 676, 755, 768
AcsI	RAATTY	1 cut(s)	829
AcyI	GRCGYC	1 cut(s)	507
AfaI	GTAC	1 cut(s)	444
AfiI	CCNNNNNNNGG	1 cut(s)	587
AgsI	TTSAA	2 cut(s)	101, 585
AhdI	GACNNNNNGTC	1 cut(s)	362
AjiI	CACGTC	1 cut(s)	610
AluBI	AGCT	2 cut(s)	395, 720
AluI	AGCT	2 cut(s)	395, 720
Alw21I	GWGCWC	2 cut(s)	122, 498
Alw26I	GTCTC	2 cut(s)	220, 665
AlwI	GGATC	4 cut(s)	663, 676, 755, 768
AlwNI	CAGNNNCTG	1 cut(s)	142
Ama87I	CYCGRG	2 cut(s)	390, 664
ApeKI	GCWGC	3 cut(s)	133, 136, 788
ApoI	RAATTY	1 cut(s)	829
AseI	ATTAAT	1 cut(s)	615
AspLEI	GCGC	1 cut(s)	147
AsuHPI	GGTGA	1 cut(s)	358
AvaI	CYCGRG	2 cut(s)	390, 664
BaeGI	GKGCMC	1 cut(s)	570
BaeI	ACNNNNGTAYC	2 cut(s)	434, 467
BamHI	GGATCC	2 cut(s)	668, 760
BauI	CACGAG	1 cut(s)	473
BbsI	GAAGAC	1 cut(s)	57
Bbv12I	GWGCWC	2 cut(s)	122, 498
BbvI	GCAGC	3 cut(s)	145, 148, 775
BccI	CCATC	3 cut(s)	326, 476, 518
BclI	TGATCA	1 cut(s)	175
BcoDI	GTCTC	2 cut(s)	220, 665
BfaI	CTAG	1 cut(s)	290
BfmI	CTRYAG	1 cut(s)	789
BfuAI	ACCTGC	1 cut(s)	823
BisI	GCNGC	3 cut(s)	134, 137, 789
BlsI	GCNGC	3 cut(s)	135, 138, 790
BmeRI	GACNNNNNGTC	1 cut(s)	362
BmeT110I	CYCGRG	2 cut(s)	390, 664
BmgBI	CACGTC	1 cut(s)	610
BmiI	GGNNCC	3 cut(s)	639, 670, 762
BmsI	GCATC	3 cut(s)	253, 272, 413
BpiI	GAAGAC	1 cut(s)	57
BpmI	CTGGAG	1 cut(s)	706
BpuEI	CTTGAG	1 cut(s)	149
BsaBI	GATNNNNATC	2 cut(s)	243, 336
BsaHI	GRCGYC	1 cut(s)	507
BsaJI	CCNNGG	1 cut(s)	756
Bsc4I	CCNNNNNNNGG	1 cut(s)	587
Bse1I	ACTGG	1 cut(s)	672
Bse8I	GATNNNNATC	2 cut(s)	243, 336
BseDI	CCNNGG	1 cut(s)	756
BseGI	GGATG	5 cut(s)	10, 263, 383, 466, 468
BseJI	GATNNNNATC	2 cut(s)	243, 336
BseLI	CCNNNNNNNGG	1 cut(s)	587
BseMII	CTCAG	1 cut(s)	670
BseNI	ACTGG	1 cut(s)	672
BseRI	GAGGAG	1 cut(s)	408
BseSI	GKGCMC	1 cut(s)	570
BseXI	GCAGC	3 cut(s)	145, 148, 775
BsgI	GTGCAG	1 cut(s)	507
BsiHKAI	GWGCWC	2 cut(s)	122, 498
BsiHKCI	CYCGRG	2 cut(s)	390, 664
BsiSI	CCGG	2 cut(s)	260, 635
BslFI	GGGAC	2 cut(s)	609, 610
BslI	CCNNNNNNNGG	1 cut(s)	587
BsmAI	GTCTC	2 cut(s)	220, 665
BsmFI	GGGAC	2 cut(s)	609, 610
BsoBI	CYCGRG	2 cut(s)	390, 664
Bsp1286I	GDGCHC	3 cut(s)	122, 498, 570
Bsp143I	GATC	4 cut(s)	175, 652, 668, 760
BspACI	CCGC	2 cut(s)	111, 632
BspCNI	CTCAG	1 cut(s)	669
BspHI	TCATGA	1 cut(s)	189
BspLI	GGNNCC	3 cut(s)	639, 670, 762
BspMAI	CTGCAG	1 cut(s)	793
BspMI	ACCTGC	1 cut(s)	823
BspPI	GGATC	4 cut(s)	663, 676, 755, 768
BsrI	ACTGG	1 cut(s)	672
BssECI	CCNNGG	1 cut(s)	756
BssMI	GATC	4 cut(s)	175, 652, 668, 760
BssNI	GRCGYC	1 cut(s)	507
BssSI	CACGAG	1 cut(s)	473
BssT1I	CCWWGG	1 cut(s)	756
Bst2BI	CACGAG	1 cut(s)	473
Bst4CI	ACNGT	4 cut(s)	41, 171, 209, 285
Bst6I	CTCTTC	1 cut(s)	721
BstACI	GRCGYC	1 cut(s)	507
BstAPI	GCANNNNNTGC	2 cut(s)	142, 815
BstDEI	CTNAG	2 cut(s)	86, 656
BstEII	GGTNACC	1 cut(s)	364
BstF5I	GGATG	5 cut(s)	10, 263, 383, 466, 468
BstHHI	GCGC	1 cut(s)	147
BstKTI	GATC	4 cut(s)	178, 655, 671, 763
BstMAI	GTCTC	2 cut(s)	220, 665
BstMBI	GATC	4 cut(s)	175, 652, 668, 760
BstMWI	GCNNNNNNNGC	7 cut(s)	142, 151, 646, 708, 717, 794, 815
BstPI	GGTNACC	1 cut(s)	364
BstSFI	CTRYAG	1 cut(s)	789
BstSLI	GKGCMC	1 cut(s)	570
BstV1I	GCAGC	3 cut(s)	145, 148, 775
BstV2I	GAAGAC	1 cut(s)	57
BstX2I	RGATCY	2 cut(s)	668, 760
BstXI	CCANNNNNNTGG	1 cut(s)	475
BstYI	RGATCY	2 cut(s)	668, 760
BtgZI	GCGATG	1 cut(s)	230
BtrI	CACGTC	1 cut(s)	610
BtsCI	GGATG	5 cut(s)	10, 263, 383, 466, 468
BtsIMutI	CAGTG	2 cut(s)	46, 214
BveI	ACCTGC	1 cut(s)	823
CaiI	CAGNNNCTG	1 cut(s)	142
CciI	TCATGA	1 cut(s)	189
CfoI	GCGC	1 cut(s)	147
CseI	GACGC	1 cut(s)	515
Csp6I	GTAC	1 cut(s)	443
CviAII	CATG	3 cut(s)	190, 543, 712
CviJI	RGCY	5 cut(s)	318, 395, 640, 702, 720
CviKI_1	RGCY	5 cut(s)	318, 395, 640, 702, 720
CviQI	GTAC	1 cut(s)	443
DdeI	CTNAG	2 cut(s)	86, 656
DpnI	GATC	4 cut(s)	177, 654, 670, 762
DpnII	GATC	4 cut(s)	175, 652, 668, 760
DriI	GACNNNNNGTC	1 cut(s)	362
Eam1104I	CTCTTC	1 cut(s)	721
Eam1105I	GACNNNNNGTC	1 cut(s)	362
EarI	CTCTTC	1 cut(s)	721
EciI	GGCGGA	1 cut(s)	621
Eco130I	CCWWGG	1 cut(s)	756
Eco32I	GATATC	1 cut(s)	338
Eco88I	CYCGRG	2 cut(s)	390, 664
Eco91I	GGTNACC	1 cut(s)	364
EcoO65I	GGTNACC	1 cut(s)	364
EcoRV	GATATC	1 cut(s)	338
EcoT14I	CCWWGG	1 cut(s)	756
EcoT22I	ATGCAT	1 cut(s)	156
ErhI	CCWWGG	1 cut(s)	756
FaeI	CATG	3 cut(s)	193, 546, 715
FaqI	GGGAC	2 cut(s)	609, 610
FatI	CATG	3 cut(s)	189, 542, 711
FauNDI	CATATG	1 cut(s)	150
FbaI	TGATCA	1 cut(s)	175
FblI	GTMKAC	1 cut(s)	36
Fnu4HI	GCNGC	3 cut(s)	134, 137, 789
FokI	GGATG	5 cut(s)	17, 250, 370, 455, 473
Fsp4HI	GCNGC	3 cut(s)	134, 137, 789
FspBI	CTAG	1 cut(s)	290
GlaI	GCGC	1 cut(s)	146
GluI	GCNGC	3 cut(s)	134, 137, 789
GsuI	CTGGAG	1 cut(s)	706
HapII	CCGG	2 cut(s)	260, 635
HgaI	GACGC	1 cut(s)	515
HhaI	GCGC	1 cut(s)	147
Hin1I	GRCGYC	1 cut(s)	507
Hin1II	CATG	3 cut(s)	193, 546, 715
Hin6I	GCGC	1 cut(s)	145
HinP1I	GCGC	1 cut(s)	145
HincII	GTYRAC	1 cut(s)	753
HindII	GTYRAC	1 cut(s)	753
HinfI	GANTC	4 cut(s)	90, 302, 464, 518
HpaI	GTTAAC	1 cut(s)	753
HpaII	CCGG	2 cut(s)	260, 635
HphI	GGTGA	1 cut(s)	358
Hpy166II	GTNNAC	3 cut(s)	37, 548, 753
Hpy188III	TCNNGA	7 cut(s)	47, 190, 595, 666, 680, 723, 764
Hpy8I	GTNNAC	3 cut(s)	37, 548, 753
Hpy99I	CGWCG	1 cut(s)	509
HpyAV	CCTTC	6 cut(s)	355, 390, 421, 433, 482, 591
HpyCH4III	ACNGT	4 cut(s)	41, 171, 209, 285
HpyCH4IV	ACGT	4 cut(s)	61, 609, 741, 799
HpyCH4V	TGCA	8 cut(s)	58, 154, 453, 524, 791, 797, 809, 818
HpyF10VI	GCNNNNNNNGC	7 cut(s)	142, 151, 646, 708, 717, 794, 815
HpyF3I	CTNAG	2 cut(s)	86, 656
HpySE526I	ACGT	4 cut(s)	61, 609, 741, 799
Hsp92I	GRCGYC	1 cut(s)	507
Hsp92II	CATG	3 cut(s)	193, 546, 715
HspAI	GCGC	1 cut(s)	145
Ksp22I	TGATCA	1 cut(s)	175
KspAI	GTTAAC	1 cut(s)	753
Kzo9I	GATC	4 cut(s)	175, 652, 668, 760
LmnI	GCTCC	3 cut(s)	421, 637, 725
Lsp1109I	GCAGC	3 cut(s)	145, 148, 775
LweI	GCATC	3 cut(s)	253, 272, 413
MaeI	CTAG	1 cut(s)	290
MaeII	ACGT	4 cut(s)	61, 609, 741, 799
MaeIII	GTNAC	2 cut(s)	364, 643
MalI	GATC	4 cut(s)	177, 654, 670, 762
MboI	GATC	4 cut(s)	175, 652, 668, 760
MboII	GAAGA	2 cut(s)	62, 738
MflI	RGATCY	2 cut(s)	668, 760
MhlI	GDGCHC	3 cut(s)	122, 498, 570
MluCI	AATT	4 cut(s)	536, 561, 574, 829
MmeI	TCCRAC	1 cut(s)	184
Mph1103I	ATGCAT	1 cut(s)	156
MseI	TTAA	4 cut(s)	18, 573, 615, 752
MslI	CAYNNNNRTG	2 cut(s)	473, 563
MspI	CCGG	2 cut(s)	260, 635
MwoI	GCNNNNNNNGC	7 cut(s)	142, 151, 646, 708, 717, 794, 815
NdeI	CATATG	1 cut(s)	150
NdeII	GATC	4 cut(s)	175, 652, 668, 760
NlaIII	CATG	3 cut(s)	193, 546, 715
NlaIV	GGNNCC	3 cut(s)	639, 670, 762
NmuCI	GTSAC	1 cut(s)	364
NsiI	ATGCAT	1 cut(s)	156
PaeR7I	CTCGAG	1 cut(s)	390
PagI	TCATGA	1 cut(s)	189
PfeI	GAWTC	4 cut(s)	90, 302, 464, 518
PkrI	GCNGC	3 cut(s)	135, 138, 790
PshBI	ATTAAT	1 cut(s)	615
Psp1406I	AACGTT	1 cut(s)	61
PspEI	GGTNACC	1 cut(s)	364
PspN4I	GGNNCC	3 cut(s)	639, 670, 762
PspXI	VCTCGAGB	1 cut(s)	390
PsrI	GAACNNNNNNTAC	2 cut(s)	27, 59
PstI	CTGCAG	1 cut(s)	793
PstNI	CAGNNNCTG	1 cut(s)	142
PsuI	RGATCY	2 cut(s)	668, 760
RsaI	GTAC	1 cut(s)	444
RsaNI	GTAC	1 cut(s)	443
RseI	CAYNNNNRTG	2 cut(s)	473, 563
SaqAI	TTAA	4 cut(s)	18, 573, 615, 752
SatI	GCNGC	3 cut(s)	134, 137, 789
Sau3AI	GATC	4 cut(s)	175, 652, 668, 760
SduI	GDGCHC	3 cut(s)	122, 498, 570
SfaNI	GCATC	3 cut(s)	253, 272, 413
SfcI	CTRYAG	1 cut(s)	789
Sfr274I	CTCGAG	1 cut(s)	390
SlaI	CTCGAG	1 cut(s)	390
SmiMI	CAYNNNNRTG	2 cut(s)	473, 563
SmlI	CTYRAG	2 cut(s)	128, 390
SmoI	CTYRAG	2 cut(s)	128, 390
Sse9I	AATT	4 cut(s)	536, 561, 574, 829
SsiI	CCGC	2 cut(s)	111, 632
SspMI	CTAG	1 cut(s)	290
StyI	CCWWGG	1 cut(s)	756
TaaI	ACNGT	4 cut(s)	41, 171, 209, 285
TaiI	ACGT	4 cut(s)	64, 612, 744, 802
TaqI	TCGA	5 cut(s)	181, 391, 421, 651, 803
TasI	AATT	4 cut(s)	536, 561, 574, 829
TfiI	GAWTC	4 cut(s)	90, 302, 464, 518
Tru1I	TTAA	4 cut(s)	18, 573, 615, 752
Tru9I	TTAA	4 cut(s)	18, 573, 615, 752
TscAI	CASTG	2 cut(s)	46, 214
TseFI	GTSAC	1 cut(s)	364
TseI	GCWGC	3 cut(s)	133, 136, 788
Tsp45I	GTSAC	1 cut(s)	364
TspDTI	ATGAA	4 cut(s)	93, 372, 531, 822
TspRI	CASTG	2 cut(s)	46, 214
VspI	ATTAAT	1 cut(s)	615
XapI	RAATTY	1 cut(s)	829
XhoI	CTCGAG	1 cut(s)	390
XmiI	GTMKAC	1 cut(s)	36
XspI	CTAG	1 cut(s)	290
Zsp2I	ATGCAT	1 cut(s)	156

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.