Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000006

Orthogroup ID

OG0011531

Physical Location & Seq

Genomic Coordinates Forward (+)

43948893 .. 43951958

Gene Structure 3066 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug06G0289100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3066 bp

ATGACTGTGAGCAACAGCACCGGCAAAGAATGCCTTGTGGTGGAGGTGGTGGCGGCCCACAACCTCATGCCCAAAGACGGCGAAGGCTCGTCTTCCCCATTCGTCGAAATTGAATTTGAAAACCAGAGGCTCCGAACCCAGGTCAAGTACAAGGACCTGAACCCGGTCTGGAACGAGAAGCTAGTCTTTAGTGTCAAGGACGTGGCTGATCTTCCCTATAGAGCCATAGAGGTCAACATTTTCAACGAGCGGAGGTCCGGCAACAGCAGAAATTTTCTGGGTAAAGTCAGAGTTTCAGGGTCCAATATTGCCAAAGAAGGAGAGGAGATTCCTCAGCTTTATACTCTCGACAAAAGAAGCCTCTTTTCTCATATCAGAGGCGAGATTAGCTTGAAGCTTTACCTTTCGACGAGAGAAGAGGTCAAGGAAGTTGGGGCTAATGGAATAGTGGGTTCCTCTGTTTCCAACTCTGCTTCTTCCTCTGGGTTTTCGAAGAAAAGCAAGAAACTTCAGGGCCAAAACTCGGTTATGGTGTCACAGAACCAGTTGATACAAGAAGCAAAGCCAACGCAGCAGGGTCAGAGCAACAATAATCACTCAAAGCCTGTGGAGAACAACCAAGGAGTGATGATGAAGCCGATTCTGATCAATACAGGCCCTGTTTCTGCAATCTCCGGCGGCGGCGGAGCAACCGGCGGAGGAGGCGGTGGCGGAGGTGGTGTTTACGGAAATGTGTTGACGGAGTTTTCGCTGAAGGAGACGCGGCCTCAGCTCGGCGGTGAGTCTTTGAAAAAGGATAAAAGCAGCTCTACTTATGACCTTGTTGAGCAAATGCAATATTTGTATGTGAAACTGGTGAAAGCTAGAGACTTCTCTGTTTTTGGTGGAGGGGAGGTTGTGGCTGAAGTGAAGTTGGGAAATTACAAAGGGATAACAAAGAGGGTGAGTTTGAACAATGTGGAGTGGGGTCAGGTGTTTGCTTTTTCTAAAGATTGCATACAGTCTTCAGTGGTGGAGGTTTTTGTGAAGGAGGGGAACAAAGATGACTTCTTGGGAAGGGTGTGGTTTGATTTGAATGAGGTTCCGAGGAGGGTGCCGCCGGATAGTCAGTTGGCGCCGCAGTGGTATAGAATGGAGGATAAGAAGGGAGACAAGTCGAAATCGGGGGAGGTGATGATTTCGATTTGGTTTGGGACTCAGGCTGATGAGGCATTTGCTGAAGCTTGGCATTCCAAGGCTGCAAATGTGAATTTTGATGGGCTTGGTTCGATTAAGTCCAAGGTTTACTTGACACCAAGGCTTTGGTACCTGAGGGTTACAGTGATTGAAGCTCAAGACATTGTTCCTGGGGAGAAAGGGTCTGCAATGATAAGGTTTCCTGAGCTTTCGGCGAAAATCCAGGTGGGGAATCAGGTCTTGAGGACTAGGATTGCGCAGCCTAGCAGTGTGAGGAGCCTCTCGAATCCTTTGTGGAATGAGGAGATGATGTTTGTGGTGGCTGAGCCGATTGAGGACTACTTGCTGGTCTGTGTTGAGGATAGGGTTGGGCCGGGACGTGACGAGGTGGCAGGGAGAGTGGTTATTCCGGTGGCAGCAATTGAAAGGCGAACGGATGATAAGCCTGTCGTTTCGAGGTGGTTCAACCTTGACAACGGCAACCGTTTCACCAATGCAGCTGGGGAGTCCAAAGTGTTGACAAGGTTTGGGTCTAGAATTCATTTGAGGGTTTCACTTGATGGAGGTTATCACGTCCTTGATGAGGCTACAATGTATAGCAGTGATCTTAAGCCGACTGATAAGAGGCTTTGGAAGCCTCACATTGGTGTGCTTGAAATGGGTATTTTGGGAGCCACTGGGCTTATGCCGATGAAGATCAAAGAAGGGAAAGGAGGGTCTAGTGATGCTTATTGTGTTGCAAAGTATGGACAGAAATGGGTTCGAACTCGAACTGTTGTTGATAGCTTGTCGCCTAAGTGGAACGAGCAGTACACGTGGGAGGTGTTTGATCCTTGCACTGTTGTTACCATTGGGGTGTTTGATAATTCTCGGATTGATAAGAACACGGCCAACAATGCTGGAGTCCGTGATTCCCGGATTGGGAAGGTTAGGATTCGATTGTCCACACTTGAGTCTGACCGAGTCTACACTCACTCATATCCGCTCTTGATGCTGCACCCTTCTGGTGTCAAGAAAATGGGCGAGCTTCATTTGGCGGTCAGGTTTTCTTGTGCCAATATGGGTAACATGTTGAACATGTACACTATGCCACTGCTTCCCAAGATGCATTTTGTGCAGCCTTTGACTGTGAATCAACTGGAAACTCTGAGATACCAGGCTATGAATGTGGTGGTGTCGAGGCTTAGCAGGACAGAGCCACCGTTGGGGAGAGAGGTGGTGGAGTACATGCTTGACCATGACTCGCATATGTGGAGCATGAGAAGGAGCAAAGCCAACTTCTTCAGGCTAGTGAATGTTCTATCAGGGCCTGTTGCTTTTGGAAGATTTGTGGAGTTGATGCGCAGTTGGCAGAAGCCAATCTGCTCTGCCTTGTTCGTTGCAACTTTCCTTTTGCTAGTTGCATTTCCGGAGCTCATAGTCCCAATGATCTTGCTGCATATGGCATTTGTGGGAATGTGGCGGTTTAAGTCCCGCCCTCGCCACCCATGTTTCATGGACACTAATCTATCCCATGCTGAGAGTGTTTATGCTGATGAGTTGGATGAGGAGTTTGATTCGTTCCCAACGAGTCGGAGTGCAGAGATTGTTAGGATGAGGTATGACCGGCTTAGGAGTGTGGCTGGGAGGATTCAAACTGTTGTTGGTGATGTGGCTACACAAGGTGAGAGGTTCCAAGCATTGCTAAGCTGGAGAGACCCAAGGGCAACATTCTTGTTTGTGATCTTCTGCTTGATTGCTGCTGTTGTGTTCTATGCTGTGCCGATTAGAGTGGTGGTGGTTTTGGTGGGATTGTATGCACTCAGGCCGCCGAGATTCAGGAGCAAGCTGCCTTCTCAGCCTTTGAGCTTCTTCAGGAGGTTGCCAACTAGGGCTGATAGCTTGTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002237 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

1021

Amino Acids

114.28

Weight (kDa)

9.35

Isoelectric Point (pI)

40.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	12 - 115	1.2e-21	C2 domain
C2	PF00168	280 - 377	4.9e-06	C2 domain
C2	PF00168	435 - 549	2.3e-13	C2 domain
C2	PF00168	608 - 721	4.4e-20	C2 domain
PRT_C	PF08372	866 - 1021	1.5e-73	Plant phosphoribosyltransferase C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0011531)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41070 FvH4_2g41070 FvH4_2g41070
malus_domestica	MD00G1143600.v1.1 MD15G1039600.v1.1
prunus_persica	Prupe.1G393100_v2.0.a1
pyrus_communis	pycom08g04720 pycom08g04730 pycom08g04740 pycom15g03700
rosa_chinensis	RchiOBHm_Chr6g0299481
rosa_laevigata	RLG00000011428
rosa_multiflora	Rmu_sc0001184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00168640
rosa_rugosa	Rorug06G0289100
rosa_samantha	Rh6AG400400 Rh6BG409100 Rh6CG414600 Rh6DG401500
rosa_wichuraiana	Rw6G035050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	2 cut(s)	1434, 2519
Acc65I	GGTACC	1 cut(s)	1305
AccB1I	GGYRCC	3 cut(s)	1093, 1114, 1305
AccBSI	CCGCTC	2 cut(s)	250, 2161
AccI	GTMKAC	1 cut(s)	2142
AccII	CGCG	1 cut(s)	763
AccIII	TCCGGA	1 cut(s)	2584
AclWI	GGATC	1 cut(s)	2000
AcoI	YGGCCR	1 cut(s)	2064
AcsI	RAATTY	4 cut(s)	113, 271, 1249, 1713
AcuI	CTGAAG	7 cut(s)	494, 773, 924, 990, 1239, 2443, 3013
AcvI	CACGTG	1 cut(s)	1992
AcyI	GRCGYC	1 cut(s)	1115
AdeI	CACNNNGTG	1 cut(s)	2840
AfaI	GTAC	5 cut(s)	149, 1307, 1988, 2258, 2402
AflII	CTTAAG	1 cut(s)	1784
AflIII	ACRYGT	3 cut(s)	1989, 2244, 2253
AjiI	CACGTC	3 cut(s)	202, 1556, 1750
AjnI	CCWGG	4 cut(s)	138, 1345, 1398, 2331
AleI	CACNNNNGTG	1 cut(s)	1821
Alw21I	GWGCWC	1 cut(s)	2592
Alw26I	GTCTC	4 cut(s)	752, 861, 1143, 2865
AlwI	GGATC	1 cut(s)	2000
AlwNI	CAGNNNCTG	2 cut(s)	659, 2486
Aor13HI	TCCGGA	1 cut(s)	2584
AoxI	GGCC	8 cut(s)	54, 514, 655, 764, 1547, 2064, 2483, 2981
ApoI	RAATTY	4 cut(s)	113, 271, 1249, 1713
ArsI	GACNNNNNNTTYG	2 cut(s)	730, 762
Asp718I	GGTACC	1 cut(s)	1305
AspLEI	GCGC	3 cut(s)	1117, 1435, 2520
AspS9I	GGNCC	8 cut(s)	55, 154, 255, 300, 514, 656, 1547, 2483
AsuC2I	CCSGG	3 cut(s)	164, 1551, 2092
AsuHPI	GGTGA	7 cut(s)	791, 868, 955, 1183, 1657, 2834, 2852
AsuII	TTCGAA	2 cut(s)	491, 1939
AvaII	GGWCC	3 cut(s)	154, 255, 300
AxyI	CCTNAGG	1 cut(s)	1310
BanI	GGYRCC	3 cut(s)	1093, 1114, 1305
BanII	GRGCYC	1 cut(s)	2592
BbrPI	CACGTG	1 cut(s)	1992
BbsI	GAAGAC	2 cut(s)	84, 996
Bbv12I	GWGCWC	1 cut(s)	2592
BbvCI	CCTCAGC	2 cut(s)	333, 768
BccI	CCATC	2 cut(s)	1250, 1730
BceAI	ACGGC	3 cut(s)	94, 1669, 2079
BcgI	CGANNNNNNTGC	2 cut(s)	1075, 1109
BciT130I	CCWGG	4 cut(s)	140, 1347, 1400, 2333
BclI	TGATCA	1 cut(s)	645
BcnI	CCSGG	3 cut(s)	164, 1551, 2092
BcoDI	GTCTC	4 cut(s)	752, 861, 1143, 2865
BfaI	CTAG	9 cut(s)	182, 864, 1425, 1440, 1710, 1896, 2465, 2573, 3045
BfmI	CTRYAG	1 cut(s)	217
BfoI	RGCGCY	1 cut(s)	1118
BfrI	CTTAAG	1 cut(s)	1784
BlpI	GCTNAGC	3 cut(s)	1500, 2360, 2861
Bme1390I	CCNGG	7 cut(s)	140, 164, 1347, 1400, 1551, 2092, 2333
Bme18I	GGWCC	3 cut(s)	154, 255, 300
BmgBI	CACGTC	3 cut(s)	202, 1556, 1750
BmgT120I	GGNCC	8 cut(s)	55, 154, 255, 300, 514, 656, 1547, 2483
BmrFI	CCNGG	7 cut(s)	140, 164, 1347, 1400, 1551, 2092, 2333
BmrI	ACTGGG	1 cut(s)	1863
BmsI	GCATC	4 cut(s)	1891, 2157, 2271, 2505
BmuI	ACTGGG	1 cut(s)	1863
BpiI	GAAGAC	2 cut(s)	84, 996
BpmI	CTGGAG	2 cut(s)	2097, 2887
Bpu10I	CCTNAGC	4 cut(s)	333, 768, 1380, 2786
Bpu1102I	GCTNAGC	3 cut(s)	1500, 2360, 2861
Bpu14I	TTCGAA	2 cut(s)	491, 1939
BpuEI	CTTGAG	3 cut(s)	1317, 1438, 2147
BpuMI	CCSGG	3 cut(s)	164, 1551, 2092
BsaAI	YACGTR	1 cut(s)	1992
BsaBI	GATNNNNATC	2 cut(s)	644, 1871
BsaHI	GRCGYC	1 cut(s)	1115
BsaI	GGTCTC	1 cut(s)	2865
BsaJI	CCNNGG	8 cut(s)	138, 619, 1085, 1233, 1278, 1295, 1346, 2876
BsaWI	WCCGGW	2 cut(s)	1585, 2584
BsaXI	ACNNNNNCTCC	8 cut(s)	705, 735, 879, 909, 1343, 1373, 1472, 1502
Bse118I	RCCGGY	3 cut(s)	20, 692, 2781
Bse1I	ACTGG	4 cut(s)	544, 858, 1858, 2319
Bse21I	CCTNAGG	1 cut(s)	1310
Bse3DI	GCAATG	2 cut(s)	1371, 2855
Bse8I	GATNNNNATC	2 cut(s)	644, 1871
BseAI	TCCGGA	1 cut(s)	2584
BseBI	CCWGG	4 cut(s)	140, 1347, 1400, 2333
BseDI	CCNNGG	8 cut(s)	138, 619, 1085, 1233, 1278, 1295, 1346, 2876
BseGI	GGATG	3 cut(s)	1618, 2725, 2775
BseJI	GATNNNNATC	2 cut(s)	644, 1871
BseMI	GCAATG	2 cut(s)	1371, 2855
BseNI	ACTGG	4 cut(s)	544, 858, 1858, 2319
BseRI	GAGGAG	6 cut(s)	338, 714, 1102, 1465, 1493, 2738
BseYI	CCCAGC	2 cut(s)	1676, 2798
BsgI	GTGCAG	3 cut(s)	2156, 2312, 2775
Bsh1236I	CGCG	1 cut(s)	763
BshFI	GGCC	8 cut(s)	56, 516, 657, 766, 1549, 2066, 2485, 2983
BshNI	GGYRCC	3 cut(s)	1093, 1114, 1305
BsiHKAI	GWGCWC	1 cut(s)	2592
BslFI	GGGAC	4 cut(s)	1207, 1566, 2582, 2632
BsmAI	GTCTC	4 cut(s)	752, 861, 1143, 2865
BsmBI	CGTCTC	1 cut(s)	752
BsmFI	GGGAC	4 cut(s)	1207, 1566, 2582, 2632
BsmI	GAATGC	2 cut(s)	35, 1228
BsnI	GGCC	8 cut(s)	56, 516, 657, 766, 1549, 2066, 2485, 2983
Bso31I	GGTCTC	1 cut(s)	2865
Bsp119I	TTCGAA	2 cut(s)	491, 1939
Bsp1286I	GDGCHC	1 cut(s)	2592
Bsp13I	TCCGGA	1 cut(s)	2584
Bsp1407I	TGTACA	1 cut(s)	2256
Bsp143I	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
Bsp1720I	GCTNAGC	3 cut(s)	1500, 2360, 2861
BspANI	GGCC	8 cut(s)	56, 516, 657, 766, 1549, 2066, 2485, 2983
BspEI	TCCGGA	1 cut(s)	2584
BspFNI	CGCG	1 cut(s)	763
BspPI	GGATC	1 cut(s)	2000
BspT104I	TTCGAA	2 cut(s)	491, 1939
BspT107I	GGYRCC	3 cut(s)	1093, 1114, 1305
BspTI	CTTAAG	1 cut(s)	1784
BspTNI	GGTCTC	1 cut(s)	2865
BsrBI	CCGCTC	2 cut(s)	250, 2161
BsrDI	GCAATG	2 cut(s)	1371, 2855
BsrFI	RCCGGY	3 cut(s)	20, 692, 2781
BsrGI	TGTACA	1 cut(s)	2256
BsrI	ACTGG	4 cut(s)	544, 858, 1858, 2319
BssAI	RCCGGY	3 cut(s)	20, 692, 2781
BssECI	CCNNGG	8 cut(s)	138, 619, 1085, 1233, 1278, 1295, 1346, 2876
BssMI	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
BssNI	GRCGYC	1 cut(s)	1115
BssT1I	CCWWGG	5 cut(s)	619, 1233, 1278, 1295, 2876
Bst2UI	CCWGG	4 cut(s)	140, 1347, 1400, 2333
Bst4CI	ACNGT	9 cut(s)	7, 1002, 1321, 1661, 1951, 2017, 2305, 2379, 2815
Bst6I	CTCTTC	1 cut(s)	411
BstACI	GRCGYC	1 cut(s)	1115
BstAFI	CTTAAG	1 cut(s)	1784
BstAPI	GCANNNNNTGC	2 cut(s)	30, 2290
BstAUI	TGTACA	1 cut(s)	2256
BstBAI	YACGTR	1 cut(s)	1992
BstBI	TTCGAA	2 cut(s)	491, 1939
BstC8I	GCNNGC	2 cut(s)	2201, 3002
BstENI	CCTNNNNNAGG	1 cut(s)	1757
BstF5I	GGATG	3 cut(s)	1618, 2725, 2775
BstFNI	CGCG	1 cut(s)	763
BstH2I	RGCGCY	1 cut(s)	1118
BstHHI	GCGC	3 cut(s)	1117, 1435, 2520
BstKTI	GATC	7 cut(s)	211, 648, 1783, 1875, 2008, 2607, 2901
BstMAI	GTCTC	4 cut(s)	752, 861, 1143, 2865
BstMBI	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
BstNI	CCWGG	4 cut(s)	140, 1347, 1400, 2333
BstNSI	RCATGY	3 cut(s)	2248, 2257, 2407
BstSCI	CCNGG	7 cut(s)	138, 162, 1345, 1398, 1549, 2090, 2331
BstSFI	CTRYAG	1 cut(s)	217
BstUI	CGCG	1 cut(s)	763
BstV2I	GAAGAC	2 cut(s)	84, 996
BstXI	CCANNNNNNTGG	1 cut(s)	1302
Bsu36I	CCTNAGG	1 cut(s)	1310
BsuRI	GGCC	8 cut(s)	56, 516, 657, 766, 1549, 2066, 2485, 2983
BtrI	CACGTC	3 cut(s)	202, 1556, 1750
BtsCI	GGATG	3 cut(s)	1618, 2725, 2775
BtsI	GCAGTG	4 cut(s)	1127, 1450, 1783, 2267
BtsIMutI	CAGTG	8 cut(s)	1014, 1127, 1326, 1450, 1783, 1851, 2013, 2267
Cac8I	GCNNGC	2 cut(s)	2201, 3002
CaiI	CAGNNNCTG	2 cut(s)	659, 2486
CfoI	GCGC	3 cut(s)	1117, 1435, 2520
Cfr10I	RCCGGY	3 cut(s)	20, 692, 2781
Cfr13I	GGNCC	8 cut(s)	55, 154, 255, 300, 514, 656, 1547, 2483
CseI	GACGC	1 cut(s)	769
Csp6I	GTAC	5 cut(s)	148, 1306, 1987, 2257, 2401
CspCI	CAANNNNNGTGG	2 cut(s)	588, 623
CviAII	CATG	9 cut(s)	67, 2245, 2254, 2404, 2414, 2434, 2664, 2671, 2690
CviQI	GTAC	5 cut(s)	148, 1306, 1987, 2257, 2401
DinI	GGCGCC	1 cut(s)	1116
DpnI	GATC	7 cut(s)	210, 647, 1782, 1874, 2007, 2606, 2900
DpnII	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
DraIII	CACNNNGTG	1 cut(s)	2840
EaeI	YGGCCR	1 cut(s)	2064
Eam1104I	CTCTTC	1 cut(s)	411
EarI	CTCTTC	1 cut(s)	411
EciI	GGCGGA	3 cut(s)	699, 711, 726
Ecl136II	GAGCTC	1 cut(s)	2590
Eco130I	CCWWGG	5 cut(s)	619, 1233, 1278, 1295, 2876
Eco24I	GRGCYC	1 cut(s)	2592
Eco31I	GGTCTC	1 cut(s)	2865
Eco47I	GGWCC	3 cut(s)	154, 255, 300
Eco53kI	GAGCTC	1 cut(s)	2590
Eco57I	CTGAAG	7 cut(s)	494, 773, 924, 990, 1239, 2443, 3013
Eco72I	CACGTG	1 cut(s)	1992
Eco81I	CCTNAGG	1 cut(s)	1310
EcoICRI	GAGCTC	1 cut(s)	2590
EcoNI	CCTNNNNNAGG	1 cut(s)	1757
EcoO109I	RGGNCCY	3 cut(s)	154, 656, 2483
EcoRI	GAATTC	1 cut(s)	1713
EcoRII	CCWGG	4 cut(s)	138, 1345, 1398, 2331
EcoT14I	CCWWGG	5 cut(s)	619, 1233, 1278, 1295, 2876
EcoT22I	ATGCAT	1 cut(s)	2286
EcoT38I	GRGCYC	1 cut(s)	2592
EgeI	GGCGCC	1 cut(s)	1116
EheI	GGCGCC	1 cut(s)	1116
ErhI	CCWWGG	5 cut(s)	619, 1233, 1278, 1295, 2876
Esp3I	CGTCTC	1 cut(s)	752
FaeI	CATG	9 cut(s)	70, 2248, 2257, 2407, 2417, 2437, 2667, 2674, 2693
FalI	AAGNNNNNCTT	6 cut(s)	18, 50, 170, 202, 1271, 1303
FaqI	GGGAC	4 cut(s)	1207, 1566, 2582, 2632
FatI	CATG	9 cut(s)	66, 2244, 2253, 2403, 2413, 2433, 2663, 2670, 2689
FauI	CCCGC	1 cut(s)	2657
FauNDI	CATATG	2 cut(s)	2424, 2616
FbaI	TGATCA	1 cut(s)	645
FblI	GTMKAC	1 cut(s)	2142
FokI	GGATG	3 cut(s)	1625, 2732, 2782
FriOI	GRGCYC	1 cut(s)	2592
FspBI	CTAG	9 cut(s)	182, 864, 1425, 1440, 1710, 1896, 2465, 2573, 3045
FspI	TGCGCA	2 cut(s)	1434, 2519
GlaI	GCGC	3 cut(s)	1116, 1434, 2519
GsaI	CCCAGC	2 cut(s)	1680, 2802
GsuI	CTGGAG	2 cut(s)	2097, 2887
HaeII	RGCGCY	1 cut(s)	1118
HaeIII	GGCC	8 cut(s)	56, 516, 657, 766, 1549, 2066, 2485, 2983
HgaI	GACGC	1 cut(s)	769
HhaI	GCGC	3 cut(s)	1117, 1435, 2520
Hin1I	GRCGYC	1 cut(s)	1115
Hin1II	CATG	9 cut(s)	70, 2248, 2257, 2407, 2417, 2437, 2667, 2674, 2693
Hin6I	GCGC	3 cut(s)	1115, 1433, 2518
HinP1I	GCGC	3 cut(s)	1115, 1433, 2518
HincII	GTYRAC	3 cut(s)	235, 738, 1695
HindII	GTYRAC	3 cut(s)	235, 738, 1695
HindIII	AAGCTT	2 cut(s)	395, 1221
HphI	GGTGA	7 cut(s)	791, 868, 955, 1183, 1657, 2834, 2852
Hpy166II	GTNNAC	9 cut(s)	235, 724, 738, 1285, 1695, 1989, 2121, 2143, 2259
Hpy8I	GTNNAC	9 cut(s)	235, 724, 738, 1285, 1695, 1989, 2121, 2143, 2259
Hpy99I	CGWCG	2 cut(s)	107, 412
HpyCH4III	ACNGT	9 cut(s)	7, 1002, 1321, 1661, 1951, 2017, 2305, 2379, 2815
HpyCH4IV	ACGT	4 cut(s)	201, 1555, 1749, 1991
HpySE526I	ACGT	4 cut(s)	201, 1555, 1749, 1991
Hsp92I	GRCGYC	1 cut(s)	1115
Hsp92II	CATG	9 cut(s)	70, 2248, 2257, 2407, 2417, 2437, 2667, 2674, 2693
HspAI	GCGC	3 cut(s)	1115, 1433, 2518
KasI	GGCGCC	1 cut(s)	1114
Kpn2I	TCCGGA	1 cut(s)	2584
KpnI	GGTACC	1 cut(s)	1309
Ksp22I	TGATCA	1 cut(s)	645
Kzo9I	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
LmnI	GCTCC	8 cut(s)	135, 686, 1452, 1847, 2430, 2442, 2587, 2997
LweI	GCATC	4 cut(s)	1891, 2157, 2271, 2505
MaeI	CTAG	9 cut(s)	182, 864, 1425, 1440, 1710, 1896, 2465, 2573, 3045
MaeII	ACGT	4 cut(s)	201, 1555, 1749, 1991
MaeIII	GTNAC	5 cut(s)	534, 1315, 1556, 2020, 2240
MalI	GATC	7 cut(s)	210, 647, 1782, 1874, 2007, 2606, 2900
MbiI	CCGCTC	2 cut(s)	250, 2161
MboI	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
MfeI	CAATTG	1 cut(s)	1596
MhlI	GDGCHC	1 cut(s)	2592
MluCI	AATT	8 cut(s)	108, 113, 271, 919, 1249, 1596, 1713, 2041
Mly113I	GGCGCC	1 cut(s)	1115
MlyI	GAGTC	8 cut(s)	791, 1189, 1691, 2088, 2138, 2148, 2411, 2755
MmeI	TCCRAC	3 cut(s)	489, 2697, 2729
Mph1103I	ATGCAT	1 cut(s)	2286
MroI	TCCGGA	1 cut(s)	2584
MseI	TTAA	3 cut(s)	1272, 1785, 2643
MslI	CAYNNNNRTG	3 cut(s)	1821, 1823, 2030
MspA1I	CMGCKG	1 cut(s)	1676
MspCI	CTTAAG	1 cut(s)	1784
MspR9I	CCNGG	7 cut(s)	140, 164, 1347, 1400, 1551, 2092, 2333
MunI	CAATTG	1 cut(s)	1596
Mva1269I	GAATGC	2 cut(s)	35, 1228
MvaI	CCWGG	4 cut(s)	140, 1347, 1400, 2333
MvnI	CGCG	1 cut(s)	763
NarI	GGCGCC	1 cut(s)	1115
NciI	CCSGG	3 cut(s)	164, 1551, 2092
NdeI	CATATG	2 cut(s)	2424, 2616
NdeII	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
NlaIII	CATG	9 cut(s)	70, 2248, 2257, 2407, 2417, 2437, 2667, 2674, 2693
NmeAIII	GCCGAG	2 cut(s)	753, 3012
NmuCI	GTSAC	2 cut(s)	534, 1556
NsbI	TGCGCA	2 cut(s)	1434, 2519
NsiI	ATGCAT	1 cut(s)	2286
NspI	RCATGY	3 cut(s)	2248, 2257, 2407
NspV	TTCGAA	2 cut(s)	491, 1939
OliI	CACNNNNGTG	1 cut(s)	1821
PciI	ACATGT	2 cut(s)	2244, 2253
PcsI	WCGNNNNNNNCGW	1 cut(s)	2741
PctI	GAATGC	2 cut(s)	35, 1228
PflFI	GACNNNGTC	1 cut(s)	2138
PfoI	TCCNGGA	1 cut(s)	2090
PleI	GAGTC	8 cut(s)	790, 1189, 1690, 2087, 2137, 2147, 2411, 2754
PluTI	GGCGCC	1 cut(s)	1118
PmaCI	CACGTG	1 cut(s)	1992
PmlI	CACGTG	1 cut(s)	1992
PpsI	GAGTC	8 cut(s)	790, 1189, 1690, 2087, 2137, 2147, 2411, 2754
Ppu21I	YACGTR	1 cut(s)	1992
PpuMI	RGGWCCY	1 cut(s)	154
PscI	ACATGT	2 cut(s)	2244, 2253
Psp124BI	GAGCTC	1 cut(s)	2592
Psp5II	RGGWCCY	1 cut(s)	154
Psp6I	CCWGG	4 cut(s)	138, 1345, 1398, 2331
PspCI	CACGTG	1 cut(s)	1992
PspFI	CCCAGC	2 cut(s)	1676, 2798
PspGI	CCWGG	4 cut(s)	138, 1345, 1398, 2331
PspPI	GGNCC	8 cut(s)	55, 154, 255, 300, 514, 656, 1547, 2483
PspPPI	RGGWCCY	1 cut(s)	154
PsrI	GAACNNNNNNTAC	2 cut(s)	1970, 2002
PstNI	CAGNNNCTG	2 cut(s)	659, 2486
PsyI	GACNNNGTC	1 cut(s)	2138
PvuII	CAGCTG	1 cut(s)	1676
RsaI	GTAC	5 cut(s)	149, 1307, 1988, 2258, 2402
RsaNI	GTAC	5 cut(s)	148, 1306, 1987, 2257, 2401
RseI	CAYNNNNRTG	3 cut(s)	1821, 1823, 2030
SacI	GAGCTC	1 cut(s)	2592
SaqAI	TTAA	3 cut(s)	1272, 1785, 2643
Sau3AI	GATC	7 cut(s)	208, 645, 1780, 1872, 2005, 2604, 2898
Sau96I	GGNCC	8 cut(s)	55, 154, 255, 300, 514, 656, 1547, 2483
SchI	GAGTC	8 cut(s)	791, 1189, 1691, 2088, 2138, 2148, 2411, 2755
ScrFI	CCNGG	7 cut(s)	140, 164, 1347, 1400, 1551, 2092, 2333
SduI	GDGCHC	1 cut(s)	2592
SfaNI	GCATC	4 cut(s)	1891, 2157, 2271, 2505
SfcI	CTRYAG	1 cut(s)	217
SfoI	GGCGCC	1 cut(s)	1116
SfuI	TTCGAA	2 cut(s)	491, 1939
SinI	GGWCC	3 cut(s)	154, 255, 300
SmiMI	CAYNNNNRTG	3 cut(s)	1821, 1823, 2030
SmlI	CTYRAG	4 cut(s)	1332, 1417, 1784, 2126
SmoI	CTYRAG	4 cut(s)	1332, 1417, 1784, 2126
Sse9I	AATT	8 cut(s)	108, 113, 271, 919, 1249, 1596, 1713, 2041
SspDI	GGCGCC	1 cut(s)	1114
SspI	AATATT	2 cut(s)	307, 839
SspMI	CTAG	9 cut(s)	182, 864, 1425, 1440, 1710, 1896, 2465, 2573, 3045
SstI	GAGCTC	1 cut(s)	2592
StyD4I	CCNGG	7 cut(s)	138, 162, 1345, 1398, 1549, 2090, 2331
StyI	CCWWGG	5 cut(s)	619, 1233, 1278, 1295, 2876
TaaI	ACNGT	9 cut(s)	7, 1002, 1321, 1661, 1951, 2017, 2305, 2379, 2815
TaiI	ACGT	4 cut(s)	204, 1558, 1752, 1994
TaqII	GACCGA	1 cut(s)	2151
TasI	AATT	8 cut(s)	108, 113, 271, 919, 1249, 1596, 1713, 2041
TatI	WGTACW	4 cut(s)	147, 1986, 2256, 2400
TauI	GCSGC	7 cut(s)	56, 681, 684, 766, 1099, 1120, 2986
Tru1I	TTAA	3 cut(s)	1272, 1785, 2643
Tru9I	TTAA	3 cut(s)	1272, 1785, 2643
TscAI	CASTG	8 cut(s)	1014, 1127, 1326, 1450, 1783, 1858, 2020, 2274
TseFI	GTSAC	2 cut(s)	534, 1556
Tsp45I	GTSAC	2 cut(s)	534, 1556
TspDTI	ATGAA	6 cut(s)	647, 1706, 1883, 2195, 2354, 2659
TspGWI	ACGGA	4 cut(s)	741, 755, 1625, 2072
TspRI	CASTG	8 cut(s)	1014, 1127, 1326, 1450, 1783, 1858, 2020, 2274
Tth111I	GACNNNGTC	1 cut(s)	2138
Vha464I	CTTAAG	1 cut(s)	1784
VpaK11BI	GGWCC	3 cut(s)	154, 255, 300
XagI	CCTNNNNNAGG	1 cut(s)	1757
XapI	RAATTY	4 cut(s)	113, 271, 1249, 1713
XbaI	TCTAGA	1 cut(s)	1709
XceI	RCATGY	3 cut(s)	2248, 2257, 2407
XmiI	GTMKAC	1 cut(s)	2142
XspI	CTAG	9 cut(s)	182, 864, 1425, 1440, 1710, 1896, 2465, 2573, 3045
Zsp2I	ATGCAT	1 cut(s)	2286

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.