Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0011531

Physical Location & Seq

Genomic Coordinates Forward (+)

2798151 .. 2799702

Gene Structure 1552 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1039600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 861 bp

ATGGATGCTCGTACGCCTAACAAACAAAAGGTTGTAGATAGTGTTCAAGAAGACTTACAACGTTTGAGGAATGAGAATGCAACCCTAAGATTCCTGCTTCAAGCTATGACCACCAGATGCTCTACACTTGAGCAGAAGCACCACCACCACCACCACCAAATGCATATCCAACAACAACCGTGTGATCATCAGCATATCATGACCACGAATATCAACAGTGCGATTACGAGCAAGAGACCGAGAACTGAAGATCATTGCGATCCGTTTCCGGCATCCCACCATAAGACATCACAGTTCCTAGTAAAAACCGACTCTAAAGACAACAGCCTTATTGTGAAAGATGGATATCAATGGAGGAAATATGGACAGAAGGTCACCAAAGACAACCCTTCATCCCCTCGAGCTTATTTCAGGTGTTCCTTCGCTCCTCGATGCCCTGTCAAAAAGAAGGTACAGAGATCCATGGAGGATAAGTCCTTCCTCATGGTGACATACGAAGGAGAGCACAACCACGACACCATCAATGATTCACTACTATTTGGACAATTTTCATGTTCAGTAGCTGCAGCTGCCGATCTTAAGAACATTATTATGCCCATTAGTGTTCCTTCAAGTAGTATCGGGCCTGATAATGACGTGATTAATATAAGTCCCTCTCCGCCGGAGCCTGTAACCCTTGATCTTAATCTCTCGGGCTTGACTAATCAAGAAAATGGAGGATTTGGAAGCCCTCAAAGCATGAATTGCGACAGAAGAGTTGAGGATTACGTTGCTTCGTTAACCAAGGATCCCAATTTCACACAGGCTTTAGCTGCGGCAGTTGCACGCTCGATTACAGGACCTGCAGACAGGCCAAATTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0002237 GO:0003674 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006355 GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009719 GO:0009725 GO:0009751 GO:0009889 GO:0010033 GO:0010200 GO:0010243 GO:0010468 GO:0010556 GO:0014070 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0031347 GO:0042221 GO:0042493 GO:0042742 GO:0042802 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046677 GO:0048583 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051252 GO:0051704 GO:0051707 GO:0060255 GO:0065007 GO:0080090 GO:0080134 GO:0098542 GO:0140110 GO:1901698 GO:1901700 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

287

Amino Acids

32.09

Weight (kDa)

8.15

Isoelectric Point (pI)

58.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	113 - 172	6.8e-24	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011531)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g41070 FvH4_2g41070 FvH4_2g41070
malus_domestica	MD00G1143600.v1.1 MD15G1039600.v1.1
prunus_persica	Prupe.1G393100_v2.0.a1
pyrus_communis	pycom08g04720 pycom08g04730 pycom08g04740 pycom15g03700
rosa_chinensis	RchiOBHm_Chr6g0299481
rosa_laevigata	RLG00000011428
rosa_multiflora	Rmu_sc0001184.1_g000007
rosa_roxburghii	Rroxscaffold_7G00168640
rosa_rugosa	Rorug06G0289100
rosa_samantha	Rh6AG400400 Rh6BG409100 Rh6CG414600 Rh6DG401500
rosa_wichuraiana	Rw6G035050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	850
AciI	CCGC	2 cut(s)	659, 815
AclI	AACGTT	1 cut(s)	61
AclWI	GGATC	4 cut(s)	254, 453, 782, 795
AcuI	CTGAAG	1 cut(s)	267
AfaI	GTAC	2 cut(s)	13, 453
AflII	CTTAAG	1 cut(s)	578
AgsI	TTSAA	3 cut(s)	47, 101, 612
AhdI	GACNNNNNGTC	1 cut(s)	371
AjiI	CACGTC	1 cut(s)	637
AluBI	AGCT	5 cut(s)	104, 404, 563, 569, 812
AluI	AGCT	5 cut(s)	104, 404, 563, 569, 812
Alw21I	GWGCWC	1 cut(s)	507
Alw26I	GTCTC	1 cut(s)	229
AlwI	GGATC	4 cut(s)	254, 453, 782, 795
AlwNI	CAGNNNCTG	2 cut(s)	563, 842
Ama87I	CYCGRG	2 cut(s)	399, 691
AoxI	GGCC	2 cut(s)	623, 851
ApeKI	GCWGC	4 cut(s)	563, 566, 569, 812
AseI	ATTAAT	1 cut(s)	642
AspS9I	GGNCC	2 cut(s)	623, 839
AsuHPI	GGTGA	2 cut(s)	367, 499
AvaI	CYCGRG	2 cut(s)	399, 691
AvaII	GGWCC	1 cut(s)	839
BamHI	GGATCC	1 cut(s)	787
BbsI	GAAGAC	1 cut(s)	57
Bbv12I	GWGCWC	1 cut(s)	507
BbvI	GCAGC	4 cut(s)	550, 556, 578, 799
BccI	CCATC	2 cut(s)	335, 527
BclI	TGATCA	1 cut(s)	184
BcoDI	GTCTC	1 cut(s)	229
BfaI	CTAG	1 cut(s)	299
BfmI	CTRYAG	2 cut(s)	564, 843
BfrI	CTTAAG	1 cut(s)	578
BfuAI	ACCTGC	1 cut(s)	850
BisI	GCNGC	5 cut(s)	564, 567, 570, 813, 816
BlsI	GCNGC	5 cut(s)	565, 568, 571, 814, 817
Bme18I	GGWCC	1 cut(s)	839
BmeRI	GACNNNNNGTC	1 cut(s)	371
BmeT110I	CYCGRG	2 cut(s)	399, 691
BmgBI	CACGTC	1 cut(s)	637
BmgT120I	GGNCC	2 cut(s)	623, 839
BmiI	GGNNCC	2 cut(s)	666, 789
BmsI	GCATC	3 cut(s)	107, 281, 422
BpiI	GAAGAC	1 cut(s)	57
BpuEI	CTTGAG	1 cut(s)	149
BsaBI	GATNNNNATC	2 cut(s)	345, 684
BsaI	GGTCTC	1 cut(s)	229
BsaJI	CCNNGG	2 cut(s)	462, 783
BsaXI	ACNNNNNCTCC	2 cut(s)	458, 488
Bse3DI	GCAATG	1 cut(s)	253
Bse8I	GATNNNNATC	2 cut(s)	345, 684
BseDI	CCNNGG	2 cut(s)	462, 783
BseGI	GGATG	3 cut(s)	10, 272, 392
BseJI	GATNNNNATC	2 cut(s)	345, 684
BseMI	GCAATG	1 cut(s)	253
BseRI	GAGGAG	1 cut(s)	417
BseXI	GCAGC	4 cut(s)	550, 556, 578, 799
BshFI	GGCC	2 cut(s)	625, 853
BsiHKAI	GWGCWC	1 cut(s)	507
BsiHKCI	CYCGRG	2 cut(s)	399, 691
BsiSI	CCGG	2 cut(s)	269, 662
BsiWI	CGTACG	1 cut(s)	11
BslFI	GGGAC	1 cut(s)	636
BsmAI	GTCTC	1 cut(s)	229
BsmFI	GGGAC	1 cut(s)	636
BsmI	GAATGC	1 cut(s)	82
BsnI	GGCC	2 cut(s)	625, 853
Bso31I	GGTCTC	1 cut(s)	229
BsoBI	CYCGRG	2 cut(s)	399, 691
Bsp1286I	GDGCHC	1 cut(s)	507
Bsp143I	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
Bsp19I	CCATGG	1 cut(s)	462
BspACI	CCGC	2 cut(s)	659, 815
BspANI	GGCC	2 cut(s)	625, 853
BspHI	TCATGA	1 cut(s)	198
BspLI	GGNNCC	2 cut(s)	666, 789
BspMAI	CTGCAG	2 cut(s)	568, 847
BspMI	ACCTGC	1 cut(s)	850
BspPI	GGATC	4 cut(s)	254, 453, 782, 795
BspTI	CTTAAG	1 cut(s)	578
BspTNI	GGTCTC	1 cut(s)	229
BsrDI	GCAATG	1 cut(s)	253
BssECI	CCNNGG	2 cut(s)	462, 783
BssMI	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
BssT1I	CCWWGG	2 cut(s)	462, 783
Bst4CI	ACNGT	3 cut(s)	180, 218, 294
Bst6I	CTCTTC	1 cut(s)	748
BstAFI	CTTAAG	1 cut(s)	578
BstAPI	GCANNNNNTGC	1 cut(s)	744
BstC8I	GCNNGC	1 cut(s)	826
BstDEI	CTNAG	1 cut(s)	86
BstDSI	CCRYGG	1 cut(s)	462
BstEII	GGTNACC	1 cut(s)	373
BstF5I	GGATG	3 cut(s)	10, 272, 392
BstKTI	GATC	7 cut(s)	187, 253, 262, 461, 577, 682, 790
BstMAI	GTCTC	1 cut(s)	229
BstMBI	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
BstMWI	GCNNNNNNNGC	5 cut(s)	569, 735, 744, 812, 821
BstPI	GGTNACC	1 cut(s)	373
BstSFI	CTRYAG	2 cut(s)	564, 843
BstV1I	GCAGC	4 cut(s)	550, 556, 578, 799
BstV2I	GAAGAC	1 cut(s)	57
BstX2I	RGATCY	2 cut(s)	458, 787
BstYI	RGATCY	2 cut(s)	458, 787
BsuRI	GGCC	2 cut(s)	625, 853
BtgI	CCRYGG	1 cut(s)	462
BtrI	CACGTC	1 cut(s)	637
BtsCI	GGATG	3 cut(s)	10, 272, 392
BtsIMutI	CAGTG	1 cut(s)	223
BveI	ACCTGC	1 cut(s)	850
Cac8I	GCNNGC	1 cut(s)	826
CaiI	CAGNNNCTG	2 cut(s)	563, 842
CciI	TCATGA	1 cut(s)	198
Cfr13I	GGNCC	2 cut(s)	623, 839
Csp6I	GTAC	2 cut(s)	12, 452
CviAII	CATG	5 cut(s)	199, 463, 484, 552, 739
CviQI	GTAC	2 cut(s)	12, 452
DdeI	CTNAG	1 cut(s)	86
DpnI	GATC	7 cut(s)	186, 252, 261, 460, 576, 681, 789
DpnII	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
DriI	GACNNNNNGTC	1 cut(s)	371
Eam1104I	CTCTTC	1 cut(s)	748
Eam1105I	GACNNNNNGTC	1 cut(s)	371
EarI	CTCTTC	1 cut(s)	748
EciI	GGCGGA	1 cut(s)	648
Eco130I	CCWWGG	2 cut(s)	462, 783
Eco31I	GGTCTC	1 cut(s)	229
Eco32I	GATATC	1 cut(s)	347
Eco47I	GGWCC	1 cut(s)	839
Eco57I	CTGAAG	1 cut(s)	267
Eco88I	CYCGRG	2 cut(s)	399, 691
Eco91I	GGTNACC	1 cut(s)	373
EcoO109I	RGGNCCY	1 cut(s)	839
EcoO65I	GGTNACC	1 cut(s)	373
EcoRV	GATATC	1 cut(s)	347
EcoT14I	CCWWGG	2 cut(s)	462, 783
EcoT22I	ATGCAT	1 cut(s)	165
ErhI	CCWWGG	2 cut(s)	462, 783
FaeI	CATG	5 cut(s)	202, 466, 487, 555, 742
FaqI	GGGAC	1 cut(s)	636
FatI	CATG	5 cut(s)	198, 462, 483, 551, 738
FbaI	TGATCA	1 cut(s)	184
Fnu4HI	GCNGC	5 cut(s)	564, 567, 570, 813, 816
FokI	GGATG	3 cut(s)	17, 259, 379
Fsp4HI	GCNGC	5 cut(s)	564, 567, 570, 813, 816
FspBI	CTAG	1 cut(s)	299
GluI	GCNGC	5 cut(s)	564, 567, 570, 813, 816
HaeIII	GGCC	2 cut(s)	625, 853
HapII	CCGG	2 cut(s)	269, 662
Hin1II	CATG	5 cut(s)	202, 466, 487, 555, 742
HincII	GTYRAC	1 cut(s)	780
HindII	GTYRAC	1 cut(s)	780
HinfI	GANTC	3 cut(s)	90, 311, 527
HpaI	GTTAAC	1 cut(s)	780
HpaII	CCGG	2 cut(s)	269, 662
HphI	GGTGA	2 cut(s)	367, 499
Hpy166II	GTNNAC	1 cut(s)	780
Hpy188III	TCNNGA	3 cut(s)	47, 199, 707
Hpy8I	GTNNAC	1 cut(s)	780
HpyAV	CCTTC	7 cut(s)	364, 399, 430, 442, 487, 491, 618
HpyCH4III	ACNGT	3 cut(s)	180, 218, 294
HpyCH4IV	ACGT	3 cut(s)	61, 636, 768
HpyCH4V	TGCA	5 cut(s)	80, 163, 566, 824, 845
HpyF10VI	GCNNNNNNNGC	5 cut(s)	569, 735, 744, 812, 821
HpyF3I	CTNAG	1 cut(s)	86
HpySE526I	ACGT	3 cut(s)	61, 636, 768
Hsp92II	CATG	5 cut(s)	202, 466, 487, 555, 742
Ksp22I	TGATCA	1 cut(s)	184
KspAI	GTTAAC	1 cut(s)	780
Kzo9I	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
LmnI	GCTCC	2 cut(s)	430, 664
Lsp1109I	GCAGC	4 cut(s)	550, 556, 578, 799
LweI	GCATC	3 cut(s)	107, 281, 422
MaeI	CTAG	1 cut(s)	299
MaeII	ACGT	3 cut(s)	61, 636, 768
MaeIII	GTNAC	3 cut(s)	373, 487, 670
MalI	GATC	7 cut(s)	186, 252, 261, 460, 576, 681, 789
MboI	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
MboII	GAAGA	3 cut(s)	62, 260, 765
MflI	RGATCY	2 cut(s)	458, 787
MhlI	GDGCHC	1 cut(s)	507
MluCI	AATT	4 cut(s)	545, 742, 793, 856
MlyI	GAGTC	1 cut(s)	305
MmeI	TCCRAC	1 cut(s)	193
Mph1103I	ATGCAT	1 cut(s)	165
MseI	TTAA	5 cut(s)	579, 642, 684, 779, 859
MslI	CAYNNNNRTG	1 cut(s)	590
MspA1I	CMGCKG	1 cut(s)	569
MspCI	CTTAAG	1 cut(s)	578
MspI	CCGG	2 cut(s)	269, 662
Mva1269I	GAATGC	1 cut(s)	82
MwoI	GCNNNNNNNGC	5 cut(s)	569, 735, 744, 812, 821
NcoI	CCATGG	1 cut(s)	462
NdeII	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
NlaIII	CATG	5 cut(s)	202, 466, 487, 555, 742
NlaIV	GGNNCC	2 cut(s)	666, 789
NmuCI	GTSAC	2 cut(s)	373, 487
NsiI	ATGCAT	1 cut(s)	165
PaeR7I	CTCGAG	1 cut(s)	399
PagI	TCATGA	1 cut(s)	198
PctI	GAATGC	1 cut(s)	82
PfeI	GAWTC	2 cut(s)	90, 527
Pfl23II	CGTACG	1 cut(s)	11
PkrI	GCNGC	5 cut(s)	565, 568, 571, 814, 817
PleI	GAGTC	1 cut(s)	305
PpsI	GAGTC	1 cut(s)	305
PpuMI	RGGWCCY	1 cut(s)	839
PshBI	ATTAAT	1 cut(s)	642
Psp1406I	AACGTT	1 cut(s)	61
Psp5II	RGGWCCY	1 cut(s)	839
PspEI	GGTNACC	1 cut(s)	373
PspLI	CGTACG	1 cut(s)	11
PspN4I	GGNNCC	2 cut(s)	666, 789
PspPI	GGNCC	2 cut(s)	623, 839
PspPPI	RGGWCCY	1 cut(s)	839
PspXI	VCTCGAGB	1 cut(s)	399
PsrI	GAACNNNNNNTAC	2 cut(s)	27, 59
PstI	CTGCAG	2 cut(s)	568, 847
PstNI	CAGNNNCTG	2 cut(s)	563, 842
PsuI	RGATCY	2 cut(s)	458, 787
PvuII	CAGCTG	1 cut(s)	569
RsaI	GTAC	2 cut(s)	13, 453
RsaNI	GTAC	2 cut(s)	12, 452
RseI	CAYNNNNRTG	1 cut(s)	590
SaqAI	TTAA	5 cut(s)	579, 642, 684, 779, 859
SatI	GCNGC	5 cut(s)	564, 567, 570, 813, 816
Sau3AI	GATC	7 cut(s)	184, 250, 259, 458, 574, 679, 787
Sau96I	GGNCC	2 cut(s)	623, 839
SchI	GAGTC	1 cut(s)	305
SduI	GDGCHC	1 cut(s)	507
SfaNI	GCATC	3 cut(s)	107, 281, 422
SfcI	CTRYAG	2 cut(s)	564, 843
Sfr274I	CTCGAG	1 cut(s)	399
SinI	GGWCC	1 cut(s)	839
SlaI	CTCGAG	1 cut(s)	399
SmiMI	CAYNNNNRTG	1 cut(s)	590
SmlI	CTYRAG	3 cut(s)	128, 399, 578
SmoI	CTYRAG	3 cut(s)	128, 399, 578
Sse9I	AATT	4 cut(s)	545, 742, 793, 856
SsiI	CCGC	2 cut(s)	659, 815
SspMI	CTAG	1 cut(s)	299
StyI	CCWWGG	2 cut(s)	462, 783
TaaI	ACNGT	3 cut(s)	180, 218, 294
TaiI	ACGT	3 cut(s)	64, 639, 771
TaqI	TCGA	3 cut(s)	400, 430, 830
TaqII	GACCGA	1 cut(s)	253
TasI	AATT	4 cut(s)	545, 742, 793, 856
TauI	GCSGC	1 cut(s)	818
TfiI	GAWTC	2 cut(s)	90, 527
Tru1I	TTAA	5 cut(s)	579, 642, 684, 779, 859
Tru9I	TTAA	5 cut(s)	579, 642, 684, 779, 859
TscAI	CASTG	1 cut(s)	223
TseFI	GTSAC	2 cut(s)	373, 487
TseI	GCWGC	4 cut(s)	563, 566, 569, 812
Tsp45I	GTSAC	2 cut(s)	373, 487
TspDTI	ATGAA	3 cut(s)	381, 540, 755
TspGWI	ACGGA	1 cut(s)	252
TspRI	CASTG	1 cut(s)	223
Vha464I	CTTAAG	1 cut(s)	578
VpaK11BI	GGWCC	1 cut(s)	839
VspI	ATTAAT	1 cut(s)	642
XhoI	CTCGAG	1 cut(s)	399
XspI	CTAG	1 cut(s)	299
Zsp2I	ATGCAT	1 cut(s)	165

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.