MD00G1176400.v1.1

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0007520
Physical Location & Seq
Forward (+)
40813519 .. 40816484
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1176400.v1.1.491

Sequence Viewer

Length: 1191 bp
ATGAACTGCTGTGGGTGTGAGTGCAACAATGCAGCCGCAGAGACTCCAAAACCCAAGATTTTGGCTGTTATTTTTGATTTGGATGGCACCCTTTTGGACACAGAGCAGGCAACAAAGGGCGTTTTCGAGGAATTCTTGGCTCGCCATGGCAAGGTTTTGGACACGGAAAGGGAGGAGAAGAAGAGGTTGGGGATGACACTCAAAGACTCGGCAGCTTCGGTTGTTGAGGACTATCATCTTCCATTCACCCCTGATCAGTTTGTCCAACAAATCATCCCCATGTACCAAGAAAAGTGGATGTACGCGGAAGCTCTACCTGGTGCTAATCGCCTTATCAAACACTTCCATGACCATGGAGTACCTATAGCTCTTGCTTCAAACTCCTTACGAGAATACATAGATGCAAAAATCTCTCACCATCGAGGTTGGAAGGAACTGTTTTCAGTAATTCTTGGCAGTGACCAGGTTAAAGCGGGGAAGCCCTCTCCATATTTATTTGAAGAGGCAGCGAAGCAAATGGGTGTGGATGCTGTTCACTGCCTTGTGATTGAAGACTCATTGGTTGGTGTTAAAGCTGCCAATGCTGCCGGAATGGAGGTAGTGGCTATTCCATCCCGTGGTGAAGCTCCTGGTTCTTCCTCCCTTGCAGATACTGTGCTTCATTCTCTTTTGGAGTTTCAACCTGAGCATTGGGGCCTTCCTCCATTTGAGGACTGGGTAGATAAGGCACTGCCAATTGAACCAATTTATTTTAGTGGTCTCTATGTCAATGGGTTTGTCTGTGAAGTCACAGGGGATGGAAGATCTGCTCTCCCGGACCAAGTTTGGGGAGTTTTCTTTGGTTGGGCTGTAGCTGTAGCTGATATGCAGAAGACCTACAGGGTAGTGGTTGGCATTGGATTGGATCGCAACAGTTCTCCTGATAAAAACATTCAATTACATGTAGTTGATGGAAACAATTGTTGTATATCCAATCAGCAAATGAAACTGCTACTTGTCGGCTACATCCGGGAATCATATACAAAGGAAGTTTCATCAATCGATGCAGAAACACTCGAGGAATGTAAGTCGATTGCAAGTGCTTCACTCGATTTGCCAATATTTAGTCACCATGGTTGTGTGCCTCTGTATCCACCACAACCTTTTTCTGTGGAAGACATGATTGCCAGTGATGAGATAGAACAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

397

Amino Acids

43.63

Weight (kDa)

4.87

Isoelectric Point (pI)

36.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 22 - 197 3.5e-27 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 23 - 203 3e-27 Haloacid dehalogenase-like hydrolase
HAD PF12710 23 - 153 4.4e-09 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 158 - 205 1.1e-06 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 617
AccII CGCG 1 cut(s) 305
AciI CCGC 3 cut(s) 36, 305, 473
AclWI GGATC 1 cut(s) 912
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 3 cut(s) 284, 302, 360
AfiI CCNNNNNNNGG 3 cut(s) 151, 617, 826
AflIII ACRYGT 1 cut(s) 940
AgsI TTSAA 6 cut(s) 378, 500, 551, 680, 740, 935
AjnI CCWGG 3 cut(s) 316, 462, 628
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 7 cut(s) 215, 311, 368, 575, 626, 854, 860
AluI AGCT 7 cut(s) 215, 311, 368, 575, 626, 854, 860
Alw26I GTCTC 2 cut(s) 35, 764
AlwI GGATC 1 cut(s) 912
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 1055
AoxI GGCC 1 cut(s) 694
ApeKI GCWGC 5 cut(s) 32, 212, 506, 575, 584
ApoI RAATTY 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 694, 817
AsuC2I CCSGG 2 cut(s) 815, 1010
AsuHPI GGTGA 4 cut(s) 238, 407, 632, 1100
AvaI CYCGRG 1 cut(s) 1055
AvaII GGWCC 1 cut(s) 817
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 3 cut(s) 558, 878, 1161
BbvI GCAGC 5 cut(s) 44, 224, 518, 562, 571
BccI CCATC 5 cut(s) 77, 426, 619, 791, 944
BciT130I CCWGG 3 cut(s) 318, 464, 630
BciVI GTATCC 1 cut(s) 1140
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 2 cut(s) 815, 1010
BcoDI GTCTC 2 cut(s) 35, 764
BfmI CTRYAG 4 cut(s) 363, 849, 855, 877
BfuI GTATCC 1 cut(s) 1140
BglII AGATCT 1 cut(s) 803
BisI GCNGC 6 cut(s) 33, 36, 213, 507, 576, 585
BlsI GCNGC 6 cut(s) 34, 37, 214, 508, 577, 586
Bme1390I CCNGG 5 cut(s) 318, 464, 630, 815, 1010
Bme18I GGWCC 1 cut(s) 817
BmeT110I CYCGRG 1 cut(s) 1055
BmgT120I GGNCC 2 cut(s) 694, 817
BmiI GGNNCC 2 cut(s) 88, 695
BmrFI CCNGG 5 cut(s) 318, 464, 630, 815, 1010
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 3 cut(s) 391, 517, 1033
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 3 cut(s) 558, 878, 1161
Bpu10I CCTNAGC 1 cut(s) 684
BpuMI CCSGG 2 cut(s) 815, 1010
Bsa29I ATCGAT 1 cut(s) 1041
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 145, 352, 616, 1111
Bsc4I CCNNNNNNNGG 3 cut(s) 151, 617, 826
Bse1I ACTGG 2 cut(s) 719, 1167
BseBI CCWGG 3 cut(s) 318, 464, 630
BseCI ATCGAT 1 cut(s) 1041
BseDI CCNNGG 4 cut(s) 145, 352, 616, 1111
BseGI GGATG 8 cut(s) 88, 198, 273, 303, 532, 611, 802, 1005
BseLI CCNNNNNNNGG 3 cut(s) 151, 617, 826
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 2 cut(s) 719, 1167
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 5 cut(s) 44, 224, 518, 562, 571
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 1 cut(s) 696
BshNI GGYRCC 1 cut(s) 86
BshVI ATCGAT 1 cut(s) 1041
BsiHKCI CYCGRG 1 cut(s) 1055
BsiSI CCGG 3 cut(s) 588, 815, 1009
BslI CCNNNNNNNGG 3 cut(s) 151, 617, 826
BsmAI GTCTC 2 cut(s) 35, 764
BsnI GGCC 1 cut(s) 696
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 1055
Bsp143I GATC 3 cut(s) 253, 803, 904
Bsp19I CCATGG 3 cut(s) 145, 352, 1111
BspACI CCGC 3 cut(s) 36, 305, 473
BspANI GGCC 1 cut(s) 696
BspCNI CTCAG 1 cut(s) 676
BspDI ATCGAT 1 cut(s) 1041
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 2 cut(s) 88, 695
BspPI GGATC 1 cut(s) 912
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 764
BsrI ACTGG 2 cut(s) 719, 1167
BssECI CCNNGG 4 cut(s) 145, 352, 616, 1111
BssMI GATC 3 cut(s) 253, 803, 904
BssT1I CCWWGG 3 cut(s) 145, 352, 1111
Bst2UI CCWGG 3 cut(s) 318, 464, 630
Bst4CI ACNGT 3 cut(s) 438, 655, 914
Bst6I CTCTTC 2 cut(s) 176, 495
BstC8I GCNNGC 2 cut(s) 108, 142
BstDEI CTNAG 1 cut(s) 684
BstDSI CCRYGG 4 cut(s) 145, 352, 616, 1111
BstF5I GGATG 8 cut(s) 88, 198, 273, 303, 532, 611, 802, 1005
BstFNI CGCG 1 cut(s) 305
BstKTI GATC 3 cut(s) 256, 806, 907
BstMAI GTCTC 2 cut(s) 35, 764
BstMBI GATC 3 cut(s) 253, 803, 904
BstMWI GCNNNNNNNGC 2 cut(s) 581, 584
BstNI CCWGG 3 cut(s) 318, 464, 630
BstNSI RCATGY 1 cut(s) 944
BstSCI CCNGG 5 cut(s) 316, 462, 628, 813, 1008
BstSFI CTRYAG 4 cut(s) 363, 849, 855, 877
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 5 cut(s) 44, 224, 518, 562, 571
BstV2I GAAGAC 3 cut(s) 558, 878, 1161
BstX2I RGATCY 1 cut(s) 803
BstXI CCANNNNNNTGG 2 cut(s) 61, 353
BstYI RGATCY 1 cut(s) 803
Bsu15I ATCGAT 1 cut(s) 1041
BsuI GTATCC 1 cut(s) 1140
BsuRI GGCC 1 cut(s) 696
BsuTUI ATCGAT 1 cut(s) 1041
BtgI CCRYGG 4 cut(s) 145, 352, 616, 1111
BtsCI GGATG 8 cut(s) 88, 198, 273, 303, 532, 611, 802, 1005
BtsI GCAGTG 3 cut(s) 463, 535, 728
BtsIMutI CAGTG 4 cut(s) 463, 535, 728, 1174
Cac8I GCNNGC 2 cut(s) 108, 142
CaiI CAGNNNCTG 1 cut(s) 653
Cfr13I GGNCC 2 cut(s) 694, 817
ClaI ATCGAT 1 cut(s) 1041
CsiI ACCWGGT 2 cut(s) 316, 462
Csp6I GTAC 3 cut(s) 283, 301, 359
CspCI CAANNNNNGTGG 2 cut(s) 275, 310
CviAII CATG 7 cut(s) 146, 280, 347, 353, 941, 1112, 1159
CviQI GTAC 3 cut(s) 283, 301, 359
DdeI CTNAG 1 cut(s) 684
DpnI GATC 3 cut(s) 255, 805, 906
DpnII GATC 3 cut(s) 253, 803, 904
Eam1104I CTCTTC 2 cut(s) 176, 495
EarI CTCTTC 2 cut(s) 176, 495
Eco130I CCWWGG 3 cut(s) 145, 352, 1111
Eco31I GGTCTC 1 cut(s) 764
Eco47I GGWCC 1 cut(s) 817
Eco88I CYCGRG 1 cut(s) 1055
EcoO109I RGGNCCY 1 cut(s) 694
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 316, 462, 628
EcoT14I CCWWGG 3 cut(s) 145, 352, 1111
ErhI CCWWGG 3 cut(s) 145, 352, 1111
FaeI CATG 7 cut(s) 149, 283, 350, 356, 944, 1115, 1162
FatI CATG 7 cut(s) 145, 279, 346, 352, 940, 1111, 1158
FauI CCCGC 1 cut(s) 466
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 6 cut(s) 33, 36, 213, 507, 576, 585
FokI GGATG 8 cut(s) 95, 205, 260, 310, 539, 598, 809, 992
Fsp4HI GCNGC 6 cut(s) 33, 36, 213, 507, 576, 585
GluI GCNGC 6 cut(s) 33, 36, 213, 507, 576, 585
HaeIII GGCC 1 cut(s) 696
HapII CCGG 3 cut(s) 588, 815, 1009
Hin1II CATG 7 cut(s) 149, 283, 350, 356, 944, 1115, 1162
HinfI GANTC 4 cut(s) 43, 206, 554, 1013
HpaII CCGG 3 cut(s) 588, 815, 1009
HphI GGTGA 4 cut(s) 238, 407, 632, 1100
Hpy166II GTNNAC 1 cut(s) 535
Hpy188III TCNNGA 1 cut(s) 920
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 2 cut(s) 424, 707
HpyCH4III ACNGT 3 cut(s) 438, 655, 914
HpyCH4V TGCA 7 cut(s) 24, 32, 404, 647, 868, 1046, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 584
HpyF3I CTNAG 1 cut(s) 684
Hsp92II CATG 7 cut(s) 149, 283, 350, 356, 944, 1115, 1162
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 253, 803, 904
LmnI GCTCC 1 cut(s) 631
Lsp1109I GCAGC 5 cut(s) 44, 224, 518, 562, 571
LweI GCATC 3 cut(s) 391, 517, 1033
MabI ACCWGGT 2 cut(s) 316, 462
MaeIII GTNAC 3 cut(s) 458, 787, 1106
MalI GATC 3 cut(s) 255, 805, 906
MboI GATC 3 cut(s) 253, 803, 904
MboII GAAGA 9 cut(s) 190, 193, 230, 512, 563, 627, 813, 883, 1166
MfeI CAATTG 2 cut(s) 735, 958
MflI RGATCY 1 cut(s) 803
MluCI AATT 6 cut(s) 131, 447, 735, 744, 935, 958
MlyI GAGTC 3 cut(s) 37, 200, 548
MmeI TCCRAC 2 cut(s) 289, 407
MseI TTAA 2 cut(s) 468, 570
MslI CAYNNNNRTG 4 cut(s) 278, 345, 351, 1116
MspI CCGG 3 cut(s) 588, 815, 1009
MspR9I CCNGG 5 cut(s) 318, 464, 630, 815, 1010
MunI CAATTG 2 cut(s) 735, 958
MvaI CCWGG 3 cut(s) 318, 464, 630
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 2 cut(s) 581, 584
NciI CCSGG 2 cut(s) 815, 1010
NcoI CCATGG 3 cut(s) 145, 352, 1111
NdeII GATC 3 cut(s) 253, 803, 904
NlaIII CATG 7 cut(s) 149, 283, 350, 356, 944, 1115, 1162
NlaIV GGNNCC 2 cut(s) 88, 695
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 3 cut(s) 458, 787, 1106
NspI RCATGY 1 cut(s) 944
PaeR7I CTCGAG 1 cut(s) 1055
PciI ACATGT 1 cut(s) 940
PfeI GAWTC 1 cut(s) 1013
PflMI CCANNNNNTGG 1 cut(s) 617
PfoI TCCNGGA 2 cut(s) 813, 1008
PkrI GCNGC 6 cut(s) 34, 37, 214, 508, 577, 586
PleI GAGTC 3 cut(s) 37, 200, 548
PpsI GAGTC 3 cut(s) 37, 200, 548
PscI ACATGT 1 cut(s) 940
Psp6I CCWGG 3 cut(s) 316, 462, 628
PspGI CCWGG 3 cut(s) 316, 462, 628
PspN4I GGNNCC 2 cut(s) 88, 695
PspPI GGNCC 2 cut(s) 694, 817
PspXI VCTCGAGB 1 cut(s) 1055
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 3 cut(s) 284, 302, 360
RsaNI GTAC 3 cut(s) 283, 301, 359
RseI CAYNNNNRTG 4 cut(s) 278, 345, 351, 1116
SaqAI TTAA 2 cut(s) 468, 570
SatI GCNGC 6 cut(s) 33, 36, 213, 507, 576, 585
Sau3AI GATC 3 cut(s) 253, 803, 904
Sau96I GGNCC 2 cut(s) 694, 817
SchI GAGTC 3 cut(s) 37, 200, 548
ScrFI CCNGG 5 cut(s) 318, 464, 630, 815, 1010
SexAI ACCWGGT 2 cut(s) 316, 462
SfaNI GCATC 3 cut(s) 391, 517, 1033
SfcI CTRYAG 4 cut(s) 363, 849, 855, 877
Sfr274I CTCGAG 1 cut(s) 1055
SinI GGWCC 1 cut(s) 817
SlaI CTCGAG 1 cut(s) 1055
SmiMI CAYNNNNRTG 4 cut(s) 278, 345, 351, 1116
SmlI CTYRAG 1 cut(s) 1055
SmoI CTYRAG 1 cut(s) 1055
Sse9I AATT 6 cut(s) 131, 447, 735, 744, 935, 958
SsiI CCGC 3 cut(s) 36, 305, 473
SspI AATATT 1 cut(s) 1101
StyD4I CCNGG 5 cut(s) 316, 462, 628, 813, 1008
StyI CCWWGG 3 cut(s) 145, 352, 1111
TaaI ACNGT 3 cut(s) 438, 655, 914
TaqI TCGA 6 cut(s) 126, 421, 1041, 1056, 1070, 1089
TasI AATT 6 cut(s) 131, 447, 735, 744, 935, 958
TauI GCSGC 1 cut(s) 38
TfiI GAWTC 1 cut(s) 1013
Tru1I TTAA 2 cut(s) 468, 570
Tru9I TTAA 2 cut(s) 468, 570
TscAI CASTG 4 cut(s) 463, 542, 735, 1174
TseFI GTSAC 3 cut(s) 458, 787, 1106
TseI GCWGC 5 cut(s) 32, 212, 506, 575, 584
Tsp45I GTSAC 3 cut(s) 458, 787, 1106
TspDTI ATGAA 4 cut(s) 17, 650, 998, 1023
TspGWI ACGGA 1 cut(s) 179
TspRI CASTG 4 cut(s) 463, 542, 735, 1174
Van91I CCANNNNNTGG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 817
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 944
XcmI CCANNNNNNNNNTGG 1 cut(s) 711
XhoI CTCGAG 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.