Prupe.3G121200_v2.0.a1

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0007520
Physical Location & Seq
Forward (+)
10740514 .. 10744776
4263 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G121200.1

Sequence Viewer

Length: 1182 bp
ATGAGCTGCTGTGACTGTGCCTCCAAACACTGCAATGCAGAGGCCCCAAAACCCAAGATCTTGGCTGTCATTTTTGATTTGGATGGGACCCTTATAGACACAGAGCGGGCTACAAAGGGGGTTTTCCAGGAATTCTTGGCTAGGTATGGGAAAGTTTTGGATAAGGAAAGGGAAGAGAAGAAGAGTTTGGGGATGACATTGAAAGACTCAGCAACTTCTGTTGTTAAGGACTATGATCTCCCATTGACCCCTGATCAGTTTATCCAAGAAATCATCCCCATGTATCAAGAAAAGTGGTTGTACTCGAAAGCTCTTCCTGGTGCTAATCGCCTAATCAAACATTTCCATGACCGTGGCGTGCCTATAGCTCTTGCTTCGAACTCCTTACGAGAATACATAGATGCAAAGATCTCTCACCATCGAGGTTGGAAAGAAAGGTTTTCGGTGATTCTTGGCAGTGACCAGGTTAAAGCGGGGAAGCCCTCGCCAGATTTATTTGAAGAGGCAGCAAAGCAAATGGGCGTGGATGCAGTTCACTGCCTTGTGATTGAAGACTCAGTGGTTGGTGTTAAAGCTGCCAATGCTGCCCGAATGGAGGTAGTCGCTGTTCCACCTCGCGGTGAAGCTACTTGTTCTTCTCTTGCAAATACTGTGCTTCATTCACTTTTGGAGTTTCAGCCTGCGCATTGGGGTCTTCCTCCATTTGAAGACTGGGTAGATAATGCACTGCCAATTGAACCAATTTATTTCAGTGGTCTCAATGTCAATGGGTTTGTCAGTGAAATCACAGAGGATGGAAGATCAACTCTCCCTGACCAAGTTTGGGGAGTTTTCTTTGGTTGGGGTGTAGCTGATATGGAGAAGACCTACAGAGTAGTGGTTGGAATTGGATTAGATTACAGTTCCTGTTCTCCTAATAAAAACATTCAAATGTATGCAGTTGATGGAAACAATTGCTGTATATCCAATCAGCAAATGAAACTGCTGCTTGTCGGCTACATCCGGGGATTGAATACCAAGGAAATTACATCCATGGATGCAGAAACACTCAAGGAATGTAAGTCTATTGCGAGTGCTTCATTGGATCTGCCAATATTTAGTCACCATGGTTGTGTGCCTCTGTATCCAGAACCTTTTTCTGTGGAGGACGTGATTGGCTGTGATGAGATACAACAATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

394

Amino Acids

43.5

Weight (kDa)

5.16

Isoelectric Point (pI)

36.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 684
AccBSI CCGCTC 1 cut(s) 106
AccII CGCG 1 cut(s) 618
AciI CCGC 3 cut(s) 106, 473, 618
AclWI GGATC 1 cut(s) 1092
AcsI RAATTY 1 cut(s) 131
AfaI GTAC 1 cut(s) 302
AfiI CCNNNNNNNGG 3 cut(s) 595, 617, 823
AgsI TTSAA 7 cut(s) 202, 500, 551, 707, 737, 929, 1012
AjiI CACGTC 1 cut(s) 1150
AjnI CCWGG 3 cut(s) 126, 316, 462
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 6, 311, 368, 575, 626, 851
AluI AGCT 6 cut(s) 6, 311, 368, 575, 626, 851
Alw26I GTCTC 1 cut(s) 761
AlwI GGATC 1 cut(s) 1092
AlwNI CAGNNNCTG 1 cut(s) 906
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 5 cut(s) 6, 506, 575, 584, 985
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 2 cut(s) 43, 87
AsuC2I CCSGG 1 cut(s) 1004
AsuHPI GGTGA 4 cut(s) 407, 457, 632, 1094
AsuII TTCGAA 1 cut(s) 377
AvaII GGWCC 1 cut(s) 87
BbsI GAAGAC 4 cut(s) 558, 686, 714, 869
BbvI GCAGC 4 cut(s) 518, 562, 571, 972
BccI CCATC 4 cut(s) 77, 426, 788, 938
BciT130I CCWGG 3 cut(s) 128, 318, 464
BciVI GTATCC 1 cut(s) 1134
BclI TGATCA 1 cut(s) 253
BcnI CCSGG 1 cut(s) 1004
BcoDI GTCTC 1 cut(s) 761
BfaI CTAG 1 cut(s) 141
BfmI CTRYAG 2 cut(s) 363, 868
BfuI GTATCC 1 cut(s) 1134
BglII AGATCT 2 cut(s) 57, 408
BisI GCNGC 5 cut(s) 7, 507, 576, 585, 986
BlsI GCNGC 5 cut(s) 8, 508, 577, 586, 987
Bme1390I CCNGG 4 cut(s) 128, 318, 464, 1004
Bme18I GGWCC 1 cut(s) 87
BmgBI CACGTC 1 cut(s) 1150
BmgT120I GGNCC 2 cut(s) 43, 87
BmiI GGNNCC 3 cut(s) 45, 88, 89
BmrFI CCNGG 4 cut(s) 128, 318, 464, 1004
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 3 cut(s) 391, 517, 1027
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 4 cut(s) 558, 686, 714, 869
Bpu14I TTCGAA 1 cut(s) 377
BpuEI CTTGAG 1 cut(s) 1034
BpuMI CCSGG 1 cut(s) 1004
BsaI GGTCTC 1 cut(s) 761
BsaJI CCNNGG 5 cut(s) 352, 1003, 1017, 1032, 1105
BsaXI ACNNNNNCTCC 2 cut(s) 5, 35
Bsc4I CCNNNNNNNGG 3 cut(s) 595, 617, 823
Bse1I ACTGG 1 cut(s) 716
Bse3DI GCAATG 1 cut(s) 40
BseBI CCWGG 3 cut(s) 128, 318, 464
BseDI CCNNGG 5 cut(s) 352, 1003, 1017, 1032, 1105
BseGI GGATG 8 cut(s) 88, 198, 273, 532, 799, 999, 1028, 1042
BseLI CCNNNNNNNGG 3 cut(s) 595, 617, 823
BseMI GCAATG 1 cut(s) 40
BseMII CTCAG 2 cut(s) 222, 570
BseNI ACTGG 1 cut(s) 716
BseXI GCAGC 4 cut(s) 518, 562, 571, 972
Bsh1236I CGCG 1 cut(s) 618
BshFI GGCC 1 cut(s) 44
BsiSI CCGG 1 cut(s) 1003
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 3 cut(s) 595, 617, 823
BsmAI GTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 761
Bsp119I TTCGAA 1 cut(s) 377
Bsp143I GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
Bsp19I CCATGG 2 cut(s) 1032, 1105
BspACI CCGC 3 cut(s) 106, 473, 618
BspANI GGCC 1 cut(s) 44
BspCNI CTCAG 2 cut(s) 221, 569
BspFNI CGCG 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 45, 88, 89
BspPI GGATC 1 cut(s) 1092
BspQI GCTCTTC 1 cut(s) 318
BspT104I TTCGAA 1 cut(s) 377
BspTNI GGTCTC 1 cut(s) 761
BsrBI CCGCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 40
BsrI ACTGG 1 cut(s) 716
BssECI CCNNGG 5 cut(s) 352, 1003, 1017, 1032, 1105
BssMI GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
BssT1I CCWWGG 3 cut(s) 1017, 1032, 1105
Bst2UI CCWGG 3 cut(s) 128, 318, 464
Bst4CI ACNGT 4 cut(s) 17, 353, 652, 902
Bst6I CTCTTC 4 cut(s) 168, 176, 318, 495
BstBI TTCGAA 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 108, 359, 681
BstDEI CTNAG 2 cut(s) 208, 556
BstDSI CCRYGG 3 cut(s) 352, 1032, 1105
BstF5I GGATG 8 cut(s) 88, 198, 273, 532, 799, 999, 1028, 1042
BstFNI CGCG 1 cut(s) 618
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 6 cut(s) 60, 238, 256, 411, 803, 1087
BstMAI GTCTC 1 cut(s) 761
BstMBI GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
BstMWI GCNNNNNNNGC 2 cut(s) 581, 584
BstNI CCWGG 3 cut(s) 128, 318, 464
BstSCI CCNGG 4 cut(s) 126, 316, 462, 1002
BstSFI CTRYAG 2 cut(s) 363, 868
BstUI CGCG 1 cut(s) 618
BstV1I GCAGC 4 cut(s) 518, 562, 571, 972
BstV2I GAAGAC 4 cut(s) 558, 686, 714, 869
BstX2I RGATCY 3 cut(s) 57, 408, 1084
BstXI CCANNNNNNTGG 2 cut(s) 61, 353
BstYI RGATCY 3 cut(s) 57, 408, 1084
BsuI GTATCC 1 cut(s) 1134
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 3 cut(s) 352, 1032, 1105
BtrI CACGTC 1 cut(s) 1150
BtsCI GGATG 8 cut(s) 88, 198, 273, 532, 799, 999, 1028, 1042
BtsI GCAGTG 4 cut(s) 28, 463, 535, 725
BtsIMutI CAGTG 7 cut(s) 28, 463, 535, 564, 725, 757, 784
Cac8I GCNNGC 3 cut(s) 108, 359, 681
CaiI CAGNNNCTG 1 cut(s) 906
CfoI GCGC 1 cut(s) 685
Cfr13I GGNCC 2 cut(s) 43, 87
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 1 cut(s) 301
CspCI CAANNNNNGTGG 2 cut(s) 275, 310
CviAII CATG 4 cut(s) 280, 347, 1033, 1106
CviQI GTAC 1 cut(s) 301
DdeI CTNAG 2 cut(s) 208, 556
DpnI GATC 6 cut(s) 59, 237, 255, 410, 802, 1086
DpnII GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
Eam1104I CTCTTC 4 cut(s) 168, 176, 318, 495
EarI CTCTTC 4 cut(s) 168, 176, 318, 495
Eco130I CCWWGG 3 cut(s) 1017, 1032, 1105
Eco31I GGTCTC 1 cut(s) 761
Eco47I GGWCC 1 cut(s) 87
EcoO109I RGGNCCY 2 cut(s) 43, 87
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 126, 316, 462
EcoT14I CCWWGG 3 cut(s) 1017, 1032, 1105
ErhI CCWWGG 3 cut(s) 1017, 1032, 1105
FaeI CATG 4 cut(s) 283, 350, 1036, 1109
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 279, 346, 1032, 1105
FauI CCCGC 2 cut(s) 99, 466
FbaI TGATCA 1 cut(s) 253
Fnu4HI GCNGC 5 cut(s) 7, 507, 576, 585, 986
FokI GGATG 8 cut(s) 95, 205, 260, 539, 806, 986, 1015, 1049
Fsp4HI GCNGC 5 cut(s) 7, 507, 576, 585, 986
FspBI CTAG 1 cut(s) 141
FspI TGCGCA 1 cut(s) 684
GlaI GCGC 1 cut(s) 684
GluI GCNGC 5 cut(s) 7, 507, 576, 585, 986
HaeIII GGCC 1 cut(s) 44
HapII CCGG 1 cut(s) 1003
HhaI GCGC 1 cut(s) 685
Hin1II CATG 4 cut(s) 283, 350, 1036, 1109
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HinfI GANTC 3 cut(s) 206, 448, 554
HpaII CCGG 1 cut(s) 1003
HphI GGTGA 4 cut(s) 407, 457, 632, 1094
Hpy166II GTNNAC 1 cut(s) 535
Hpy188III TCNNGA 2 cut(s) 287, 1127
Hpy8I GTNNAC 1 cut(s) 535
HpyCH4III ACNGT 4 cut(s) 17, 353, 652, 902
HpyCH4IV ACGT 1 cut(s) 1149
HpyCH4V TGCA 8 cut(s) 33, 38, 404, 530, 644, 725, 938, 1040
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 584
HpyF3I CTNAG 2 cut(s) 208, 556
HpySE526I ACGT 1 cut(s) 1149
Hsp92II CATG 4 cut(s) 283, 350, 1036, 1109
HspAI GCGC 1 cut(s) 683
KflI GGGWCCC 1 cut(s) 87
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
LguI GCTCTTC 1 cut(s) 318
Lsp1109I GCAGC 4 cut(s) 518, 562, 571, 972
LweI GCATC 3 cut(s) 391, 517, 1027
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 1 cut(s) 141
MaeII ACGT 1 cut(s) 1149
MaeIII GTNAC 3 cut(s) 11, 458, 1100
MalI GATC 6 cut(s) 59, 237, 255, 410, 802, 1086
MbiI CCGCTC 1 cut(s) 106
MboI GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
MfeI CAATTG 2 cut(s) 732, 952
MflI RGATCY 3 cut(s) 57, 408, 1084
MluCI AATT 6 cut(s) 131, 732, 741, 885, 952, 1023
MlyI GAGTC 2 cut(s) 200, 548
MmeI TCCRAC 2 cut(s) 407, 862
MseI TTAA 3 cut(s) 225, 468, 570
MslI CAYNNNNRTG 6 cut(s) 33, 278, 345, 351, 929, 1110
MspI CCGG 1 cut(s) 1003
MspR9I CCNGG 4 cut(s) 128, 318, 464, 1004
MunI CAATTG 2 cut(s) 732, 952
MvaI CCWGG 3 cut(s) 128, 318, 464
MvnI CGCG 1 cut(s) 618
MwoI GCNNNNNNNGC 2 cut(s) 581, 584
NciI CCSGG 1 cut(s) 1004
NcoI CCATGG 2 cut(s) 1032, 1105
NdeII GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
NlaIII CATG 4 cut(s) 283, 350, 1036, 1109
NlaIV GGNNCC 3 cut(s) 45, 88, 89
NmuCI GTSAC 3 cut(s) 11, 458, 1100
NsbI TGCGCA 1 cut(s) 684
NspV TTCGAA 1 cut(s) 377
PciSI GCTCTTC 1 cut(s) 318
PfeI GAWTC 1 cut(s) 448
PfoI TCCNGGA 1 cut(s) 126
PkrI GCNGC 5 cut(s) 8, 508, 577, 586, 987
PleI GAGTC 2 cut(s) 200, 548
PpsI GAGTC 2 cut(s) 200, 548
PpuMI RGGWCCY 1 cut(s) 87
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 3 cut(s) 126, 316, 462
PspGI CCWGG 3 cut(s) 126, 316, 462
PspN4I GGNNCC 3 cut(s) 45, 88, 89
PspPI GGNCC 2 cut(s) 43, 87
PspPPI RGGWCCY 1 cut(s) 87
PsrI GAACNNNNNNTAC 2 cut(s) 591, 623
PstNI CAGNNNCTG 1 cut(s) 906
PsuI RGATCY 3 cut(s) 57, 408, 1084
RsaI GTAC 1 cut(s) 302
RsaNI GTAC 1 cut(s) 301
RseI CAYNNNNRTG 6 cut(s) 33, 278, 345, 351, 929, 1110
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 3 cut(s) 225, 468, 570
SatI GCNGC 5 cut(s) 7, 507, 576, 585, 986
Sau3AI GATC 6 cut(s) 57, 235, 253, 408, 800, 1084
Sau96I GGNCC 2 cut(s) 43, 87
SchI GAGTC 2 cut(s) 200, 548
ScrFI CCNGG 4 cut(s) 128, 318, 464, 1004
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 3 cut(s) 391, 517, 1027
SfcI CTRYAG 2 cut(s) 363, 868
SfuI TTCGAA 1 cut(s) 377
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 6 cut(s) 33, 278, 345, 351, 929, 1110
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
Sse9I AATT 6 cut(s) 131, 732, 741, 885, 952, 1023
SsiI CCGC 3 cut(s) 106, 473, 618
SspI AATATT 1 cut(s) 1095
SspMI CTAG 1 cut(s) 141
StyD4I CCNGG 4 cut(s) 126, 316, 462, 1002
StyI CCWWGG 3 cut(s) 1017, 1032, 1105
TaaI ACNGT 4 cut(s) 17, 353, 652, 902
TaiI ACGT 1 cut(s) 1152
TaqI TCGA 3 cut(s) 305, 377, 421
TasI AATT 6 cut(s) 131, 732, 741, 885, 952, 1023
TatI WGTACW 1 cut(s) 300
TfiI GAWTC 1 cut(s) 448
Tru1I TTAA 3 cut(s) 225, 468, 570
Tru9I TTAA 3 cut(s) 225, 468, 570
TscAI CASTG 7 cut(s) 35, 463, 542, 564, 732, 757, 784
TseFI GTSAC 3 cut(s) 11, 458, 1100
TseI GCWGC 5 cut(s) 6, 506, 575, 584, 985
Tsp45I GTSAC 3 cut(s) 11, 458, 1100
TspDTI ATGAA 3 cut(s) 647, 992, 1068
TspRI CASTG 7 cut(s) 35, 463, 542, 564, 732, 757, 784
VpaK11BI GGWCC 1 cut(s) 87
XapI RAATTY 1 cut(s) 131
XcmI CCANNNNNNNNNTGG 1 cut(s) 708
XspI CTAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.