Rh2DG365500

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0007520
Physical Location & Seq
Reverse (-)
51522170 .. 51526018
3849 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG365500.1

Sequence Viewer

Length: 801 bp
ATGAGCTGCTGCGACTGTGCATCCAATCACTGCAATACAGAGACCCCAAAACCCAAGATTTTGGCCATCATTCTTGATTTGGATGGGACCCTTTTGGACACAGAGCGGGCTACAAAGAGCGTCTTCAAGGAGTTTTTGGCCAAGTATGGCAAGACATTGAATAAGGAAAGGGAAGAGAAGAGGGAGATTGGGAAGACACTCAAAGACTCGTCTACCGCCACTGTTGAGGACTATGATCTTCCATTGACTCCTGACCAGTTTATTGAAGAAATCATCCCCATGTATCAACAAAAGTGGAAGCACGTGAAAGCTCTTCCTGGTGCTACCCGTCTTATCAAACATCTCCATGACCATGGAGTACCTACTGCTCTTGCTTCAAATTCCTTGCGCGAATATATAAATGCAAAGATCTCTGTCCATCAAGGTTGGCAGGGATGGTTTTCAGTAATCGTTGGCAGTGACCAGGTTAAAGCGGGCAAGCCCTCTCCAGATTTATTTGAAGAGGCAGCAAGGCAGATGGATGTAGATGCAGCTCACTGCCTTGTGATCGAAGACTCGATGGTTGGCGTTAAAGCTGCCAATGCTGCCGGAATGGAGGTAGTGGCTGTTCCACCCCATGGTGAAGCTGCTGGTTGTTCTGCCCTTGCAAACACTGTGCTCCATTCACTTTTGGAGTTTCGACCTGAGCATTGGGGTCTTCCTCCTTTTGAAGATTGGGTAGATAATGCATTGCCAATTGAAGCAATTAATTTGAGTGGTCTTTATGTTAACGGGTTTGTCATTGAAGACACAGGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

266

Amino Acids

29.21

Weight (kDa)

5.19

Isoelectric Point (pI)

37.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 22 - 197 9.5e-27 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 23 - 203 3.5e-30 Haloacid dehalogenase-like hydrolase
HAD PF12710 23 - 153 4.5e-08 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 158 - 203 2.4e-07 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 617
AccBSI CCGCTC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 212
AccII CGCG 1 cut(s) 390
AciI CCGC 3 cut(s) 106, 216, 473
AcoI YGGCCR 2 cut(s) 63, 138
AcsI RAATTY 1 cut(s) 379
AcvI CACGTG 1 cut(s) 304
AfaI GTAC 1 cut(s) 360
AfiI CCNNNNNNNGG 1 cut(s) 617
AgsI TTSAA 8 cut(s) 127, 160, 266, 378, 500, 710, 740, 785
AjnI CCWGG 2 cut(s) 316, 462
AluBI AGCT 5 cut(s) 6, 311, 533, 575, 626
AluI AGCT 5 cut(s) 6, 311, 533, 575, 626
Alw21I GWGCWC 1 cut(s) 660
Alw26I GTCTC 1 cut(s) 35
AoxI GGCC 2 cut(s) 63, 138
ApeKI GCWGC 7 cut(s) 6, 9, 506, 530, 575, 584, 626
ApoI RAATTY 1 cut(s) 379
AseI ATTAAT 1 cut(s) 747
AspLEI GCGC 1 cut(s) 390
AspS9I GGNCC 1 cut(s) 87
AsuHPI GGTGA 1 cut(s) 632
AvaII GGWCC 1 cut(s) 87
BalI TGGCCA 2 cut(s) 65, 140
BbrPI CACGTG 1 cut(s) 304
BbsI GAAGAC 5 cut(s) 115, 200, 558, 689, 792
Bbv12I GWGCWC 1 cut(s) 660
BbvI GCAGC 5 cut(s) 518, 542, 562, 571, 613
BccI CCATC 6 cut(s) 74, 77, 426, 429, 511, 553
BciT130I CCWGG 2 cut(s) 318, 464
BcoDI GTCTC 1 cut(s) 35
BglII AGATCT 1 cut(s) 408
BisI GCNGC 7 cut(s) 7, 10, 507, 531, 576, 585, 627
BlsI GCNGC 7 cut(s) 8, 11, 508, 532, 577, 586, 628
Bme1390I CCNGG 2 cut(s) 318, 464
Bme18I GGWCC 1 cut(s) 87
BmgT120I GGNCC 1 cut(s) 87
BmiI GGNNCC 2 cut(s) 88, 89
BmrFI CCNGG 2 cut(s) 318, 464
BmsI GCATC 2 cut(s) 29, 517
BpiI GAAGAC 5 cut(s) 115, 200, 558, 689, 792
BpmI CTGGAG 1 cut(s) 471
Bpu10I CCTNAGC 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 352, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 728
BseBI CCWGG 2 cut(s) 318, 464
BseDI CCNNGG 2 cut(s) 352, 616
BseGI GGATG 5 cut(s) 20, 88, 273, 440, 526
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 1 cut(s) 256
BseXI GCAGC 5 cut(s) 518, 542, 562, 571, 613
Bsh1236I CGCG 1 cut(s) 390
BshFI GGCC 2 cut(s) 65, 140
BsiHKAI GWGCWC 1 cut(s) 660
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 1 cut(s) 35
BsmFI GGGAC 1 cut(s) 100
BsnI GGCC 2 cut(s) 65, 140
Bso31I GGTCTC 1 cut(s) 35
Bsp1286I GDGCHC 1 cut(s) 660
Bsp143I GATC 3 cut(s) 235, 408, 546
Bsp19I CCATGG 2 cut(s) 352, 616
BspACI CCGC 3 cut(s) 106, 216, 473
BspANI GGCC 2 cut(s) 65, 140
BspCNI CTCAG 1 cut(s) 676
BspFNI CGCG 1 cut(s) 390
BspLI GGNNCC 2 cut(s) 88, 89
BspQI GCTCTTC 1 cut(s) 318
BspTNI GGTCTC 1 cut(s) 35
BsrBI CCGCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 2 cut(s) 352, 616
BssMI GATC 3 cut(s) 235, 408, 546
BssT1I CCWWGG 2 cut(s) 352, 616
Bst2UI CCWGG 2 cut(s) 318, 464
Bst4CI ACNGT 3 cut(s) 17, 223, 655
Bst6I CTCTTC 4 cut(s) 168, 173, 318, 495
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 108, 475, 479
BstDEI CTNAG 1 cut(s) 684
BstDSI CCRYGG 2 cut(s) 352, 616
BstF5I GGATG 5 cut(s) 20, 88, 273, 440, 526
BstFNI CGCG 1 cut(s) 390
BstHHI GCGC 1 cut(s) 390
BstKTI GATC 3 cut(s) 238, 411, 549
BstMAI GTCTC 1 cut(s) 35
BstMBI GATC 3 cut(s) 235, 408, 546
BstMWI GCNNNNNNNGC 2 cut(s) 581, 584
BstNI CCWGG 2 cut(s) 318, 464
BstSCI CCNGG 2 cut(s) 316, 462
BstUI CGCG 1 cut(s) 390
BstV1I GCAGC 5 cut(s) 518, 542, 562, 571, 613
BstV2I GAAGAC 5 cut(s) 115, 200, 558, 689, 792
BstX2I RGATCY 1 cut(s) 408
BstXI CCANNNNNNTGG 2 cut(s) 61, 353
BstYI RGATCY 1 cut(s) 408
BsuRI GGCC 2 cut(s) 65, 140
BtgI CCRYGG 2 cut(s) 352, 616
BtsCI GGATG 5 cut(s) 20, 88, 273, 440, 526
BtsI GCAGTG 3 cut(s) 28, 463, 535
BtsIMutI CAGTG 5 cut(s) 28, 219, 463, 535, 651
Cac8I GCNNGC 3 cut(s) 108, 475, 479
CfoI GCGC 1 cut(s) 390
Cfr13I GGNCC 1 cut(s) 87
CseI GACGC 1 cut(s) 109
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 1 cut(s) 359
CspCI CAANNNNNGTGG 2 cut(s) 275, 310
CviAII CATG 4 cut(s) 280, 347, 353, 617
CviQI GTAC 1 cut(s) 359
DdeI CTNAG 1 cut(s) 684
DpnI GATC 3 cut(s) 237, 410, 548
DpnII GATC 3 cut(s) 235, 408, 546
EaeI YGGCCR 2 cut(s) 63, 138
Eam1104I CTCTTC 4 cut(s) 168, 173, 318, 495
EarI CTCTTC 4 cut(s) 168, 173, 318, 495
Eco130I CCWWGG 2 cut(s) 352, 616
Eco31I GGTCTC 1 cut(s) 35
Eco47I GGWCC 1 cut(s) 87
Eco72I CACGTG 1 cut(s) 304
EcoO109I RGGNCCY 1 cut(s) 87
EcoRII CCWGG 2 cut(s) 316, 462
EcoT14I CCWWGG 2 cut(s) 352, 616
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 2 cut(s) 352, 616
FaeI CATG 4 cut(s) 283, 350, 356, 620
FaiI YATR 9 cut(s) 147, 234, 281, 348, 354, 396, 398, 618, 765
FalI AAGNNNNNCTT 2 cut(s) 107, 139
FaqI GGGAC 1 cut(s) 100
FatI CATG 4 cut(s) 279, 346, 352, 616
FauI CCCGC 2 cut(s) 99, 466
FblI GTMKAC 1 cut(s) 212
Fnu4HI GCNGC 7 cut(s) 7, 10, 507, 531, 576, 585, 627
FokI GGATG 5 cut(s) 7, 95, 260, 447, 533
Fsp4HI GCNGC 7 cut(s) 7, 10, 507, 531, 576, 585, 627
GlaI GCGC 1 cut(s) 389
GluI GCNGC 7 cut(s) 7, 10, 507, 531, 576, 585, 627
GsuI CTGGAG 1 cut(s) 471
HaeIII GGCC 2 cut(s) 65, 140
HapII CCGG 1 cut(s) 588
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 390
Hin1II CATG 4 cut(s) 283, 350, 356, 620
Hin6I GCGC 1 cut(s) 388
HinP1I GCGC 1 cut(s) 388
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 3 cut(s) 206, 247, 554
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 1 cut(s) 588
HphI GGTGA 1 cut(s) 632
Hpy166II GTNNAC 2 cut(s) 213, 769
Hpy188III TCNNGA 3 cut(s) 74, 251, 488
Hpy8I GTNNAC 2 cut(s) 213, 769
HpyCH4III ACNGT 3 cut(s) 17, 223, 655
HpyCH4IV ACGT 1 cut(s) 303
HpyCH4V TGCA 6 cut(s) 20, 33, 404, 530, 647, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 581, 584
HpyF3I CTNAG 1 cut(s) 684
HpySE526I ACGT 1 cut(s) 303
Hsp92II CATG 4 cut(s) 283, 350, 356, 620
HspAI GCGC 1 cut(s) 388
KflI GGGWCCC 1 cut(s) 87
KspAI GTTAAC 1 cut(s) 769
Kzo9I GATC 3 cut(s) 235, 408, 546
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 663
Lsp1109I GCAGC 5 cut(s) 518, 542, 562, 571, 613
LweI GCATC 2 cut(s) 29, 517
MabI ACCWGGT 1 cut(s) 462
MaeII ACGT 1 cut(s) 303
MaeIII GTNAC 1 cut(s) 458
MalI GATC 3 cut(s) 237, 410, 548
MbiI CCGCTC 1 cut(s) 106
MboI GATC 3 cut(s) 235, 408, 546
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 660
MlsI TGGCCA 2 cut(s) 65, 140
MluCI AATT 4 cut(s) 379, 735, 744, 748
MluNI TGGCCA 2 cut(s) 65, 140
MlyI GAGTC 3 cut(s) 200, 241, 548
MnlI CCTC 6 cut(s) 174, 220, 493, 496, 589, 711
Mox20I TGGCCA 2 cut(s) 65, 140
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 2 cut(s) 65, 140
MseI TTAA 4 cut(s) 468, 570, 747, 768
MslI CAYNNNNRTG 3 cut(s) 278, 345, 351
Msp20I TGGCCA 2 cut(s) 65, 140
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 2 cut(s) 318, 464
MunI CAATTG 1 cut(s) 735
MvaI CCWGG 2 cut(s) 318, 464
MvnI CGCG 1 cut(s) 390
MwoI GCNNNNNNNGC 2 cut(s) 581, 584
NcoI CCATGG 2 cut(s) 352, 616
NdeII GATC 3 cut(s) 235, 408, 546
NlaIII CATG 4 cut(s) 283, 350, 356, 620
NlaIV GGNNCC 2 cut(s) 88, 89
NmuCI GTSAC 1 cut(s) 458
NsiI ATGCAT 1 cut(s) 730
PciSI GCTCTTC 1 cut(s) 318
PflMI CCANNNNNTGG 1 cut(s) 617
PkrI GCNGC 7 cut(s) 8, 11, 508, 532, 577, 586, 628
PleI GAGTC 3 cut(s) 200, 241, 548
PmaCI CACGTG 1 cut(s) 304
PmlI CACGTG 1 cut(s) 304
PpsI GAGTC 3 cut(s) 200, 241, 548
Ppu21I YACGTR 1 cut(s) 304
PpuMI RGGWCCY 1 cut(s) 87
PshBI ATTAAT 1 cut(s) 747
Psp5II RGGWCCY 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 316, 462
PspCI CACGTG 1 cut(s) 304
PspGI CCWGG 2 cut(s) 316, 462
PspN4I GGNNCC 2 cut(s) 88, 89
PspPI GGNCC 1 cut(s) 87
PspPPI RGGWCCY 1 cut(s) 87
PsrI GAACNNNNNNTAC 2 cut(s) 591, 623
PsuI RGATCY 1 cut(s) 408
RsaI GTAC 1 cut(s) 360
RsaNI GTAC 1 cut(s) 359
RseI CAYNNNNRTG 3 cut(s) 278, 345, 351
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 4 cut(s) 468, 570, 747, 768
SatI GCNGC 7 cut(s) 7, 10, 507, 531, 576, 585, 627
Sau3AI GATC 3 cut(s) 235, 408, 546
Sau96I GGNCC 1 cut(s) 87
SchI GAGTC 3 cut(s) 200, 241, 548
ScrFI CCNGG 2 cut(s) 318, 464
SduI GDGCHC 1 cut(s) 660
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 2 cut(s) 29, 517
SinI GGWCC 1 cut(s) 87
SmiMI CAYNNNNRTG 3 cut(s) 278, 345, 351
Sse9I AATT 4 cut(s) 379, 735, 744, 748
SsiI CCGC 3 cut(s) 106, 216, 473
StyD4I CCNGG 2 cut(s) 316, 462
StyI CCWWGG 2 cut(s) 352, 616
TaaI ACNGT 3 cut(s) 17, 223, 655
TaiI ACGT 1 cut(s) 306
TaqI TCGA 3 cut(s) 549, 557, 679
TasI AATT 4 cut(s) 379, 735, 744, 748
Tru1I TTAA 4 cut(s) 468, 570, 747, 768
Tru9I TTAA 4 cut(s) 468, 570, 747, 768
TscAI CASTG 5 cut(s) 35, 226, 463, 542, 658
TseFI GTSAC 1 cut(s) 458
TseI GCWGC 7 cut(s) 6, 9, 506, 530, 575, 584, 626
Tsp45I GTSAC 1 cut(s) 458
TspRI CASTG 5 cut(s) 35, 226, 463, 542, 658
Van91I CCANNNNNTGG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 87
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 212
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.