pycom17g23430

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0007520
Physical Location & Seq
Forward (+)
21815540 .. 21818417
2878 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g23430.8

Sequence Viewer

Length: 1182 bp
ATGAGCTGCTGCGAGTGTGAGTGCAACAATGGAGATGCAGAGAACCCAAAACCCAATATTTTGGCTGTCATTTTCGATTTGGATGGCACCCTTTTGGACACAGAGCGGGCAACAAGGGGCGTGTTCGAGGAATTCTTGGCCCGGAATGGCAAAGTATTGGACAAGGAAAGGGAAGAGAAGAAGAGGTTGGGGATGACACTCAAGGACTCCGCAGCTTCGGTTGTTAAGGACTATCATCTCCCATTCACCCCTGATGAGTTTGTCCAACAAATCATCCCCATGTACCAGGAAAAGTGGAAGTATGCGAAAGCTCTTCCTGGTGCTAATCGCGTTATCAAACACTTCCATGACCACGGAGTACCTATGGCTCTTGCTTCAAACTCCTTACGGGAATACATAGAAGCAAAAATCTCTCACCATCGAGGTTGGAAGGAACTGTTTTCAGTAATTCTTGGCAGTGACCAGGTTAAAGCAGGGAAGCCCGCTCCAGATTTATTTGAAGAGGCAGCGAAGCAAATGGGTGTGGATGCAGTTCATTGCCTAGTGATTGAAGACTCAGTGGTTGGTGTTAAAGCTGCCAAAGCCGCCGGAATGGAGGTAGTGGCTGTCCCATCCCGTGGTGAAGCTGCCGGTTGTTCTTCCCTTGCAGATACTGTACTTCATTCTCTTTTGGAGTTTCAGCCCGAACATTGGGGTCTTCCTCAATTTGAAGACTGGGTAGATAAGGCACTACCGATTGAACCAATTTATTTTAGTGGTCTCTATGCCAATGGGTTTGTCAGTGAAGTCACAGAGGATGGAAGATCTGCTCTCCCCGACCAAGTCTGGGGAGTTTTCTTCGGTTGGGCTGTAGCTGATATGCAGAAGACCTACAGGGTAGTGGTTGCCATTGGATTCGATCGCAATAGTTCACCTAATAAAAACATTCAAATGCATATAGTTGATGGAAACAATTGCTGTATATCCAATCAGCAAATGAAACTGCTACTTGTCGGCTACATCCGGGAATCAAATACCAAGGAAATTTCATCCATGGATGCAGAAGTACTCGAGGAATGTAAGTTGATTGCAAGCGCCTCGCTCGATCTGCCAATATTTAGTCGCCATGGTTGTGTGCCTTTGGTTCCACAATCTTTTTCTGTGGAAGACATGACTTCGAGTGATGAGATAGAACGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

394

Amino Acids

43.6

Weight (kDa)

5.04

Isoelectric Point (pI)

45.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 617
AccBSI CCGCTC 2 cut(s) 106, 485
AccII CGCG 1 cut(s) 330
AciI CCGC 4 cut(s) 106, 210, 483, 585
AcsI RAATTY 2 cut(s) 131, 1023
AfaI GTAC 4 cut(s) 284, 360, 657, 1047
AfiI CCNNNNNNNGG 3 cut(s) 617, 690, 826
AgsI TTSAA 6 cut(s) 378, 500, 551, 710, 740, 929
AjnI CCWGG 3 cut(s) 285, 316, 462
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 6 cut(s) 6, 215, 311, 575, 626, 854
AluI AGCT 6 cut(s) 6, 215, 311, 575, 626, 854
Alw26I GTCTC 1 cut(s) 764
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 1049
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 6 cut(s) 6, 9, 212, 506, 575, 626
ApoI RAATTY 2 cut(s) 131, 1023
AspLEI GCGC 1 cut(s) 1076
AspS9I GGNCC 1 cut(s) 139
AsuC2I CCSGG 2 cut(s) 142, 1004
AsuHPI GGTGA 4 cut(s) 238, 407, 632, 903
AvaI CYCGRG 1 cut(s) 1049
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BbvI GCAGC 4 cut(s) 224, 518, 562, 613
BccI CCATC 5 cut(s) 77, 426, 619, 791, 938
BcgI CGANNNNNNTGC 2 cut(s) 1059, 1093
BciT130I CCWGG 3 cut(s) 287, 318, 464
BcnI CCSGG 2 cut(s) 142, 1004
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 2 cut(s) 849, 871
BfoI RGCGCY 1 cut(s) 1077
BglII AGATCT 1 cut(s) 803
BisI GCNGC 7 cut(s) 7, 10, 213, 507, 576, 585, 627
BlsI GCNGC 7 cut(s) 8, 11, 214, 508, 577, 586, 628
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 5 cut(s) 142, 287, 318, 464, 1004
BmeT110I CYCGRG 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 2 cut(s) 88, 1125
BmrFI CCNGG 5 cut(s) 142, 287, 318, 464, 1004
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 3 cut(s) 25, 517, 1027
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BpmI CTGGAG 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 185
BpuMI CCSGG 2 cut(s) 142, 1004
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 5 cut(s) 352, 616, 1017, 1032, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 617, 690, 826
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 3 cut(s) 287, 318, 464
BseDI CCNNGG 5 cut(s) 352, 616, 1017, 1032, 1105
BseGI GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BseLI CCNNNNNNNGG 3 cut(s) 617, 690, 826
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 719
BseXI GCAGC 4 cut(s) 224, 518, 562, 613
Bsh1236I CGCG 1 cut(s) 330
Bsh1285I CGRYCG 1 cut(s) 901
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 86
BsiEI CGRYCG 1 cut(s) 901
BsiHKCI CYCGRG 1 cut(s) 1049
BsiSI CCGG 4 cut(s) 142, 588, 630, 1003
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 3 cut(s) 617, 690, 826
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 1049
Bsp143I GATC 3 cut(s) 803, 898, 1084
Bsp19I CCATGG 2 cut(s) 1032, 1105
BspACI CCGC 4 cut(s) 106, 210, 483, 585
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 1 cut(s) 569
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 88, 1125
BspQI GCTCTTC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 764
BsrBI CCGCTC 2 cut(s) 106, 485
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 5 cut(s) 352, 616, 1017, 1032, 1105
BssMI GATC 3 cut(s) 803, 898, 1084
BssT1I CCWWGG 3 cut(s) 1017, 1032, 1105
Bst2UI CCWGG 3 cut(s) 287, 318, 464
Bst4CI ACNGT 2 cut(s) 438, 655
Bst6I CTCTTC 4 cut(s) 168, 176, 318, 495
BstC8I GCNNGC 3 cut(s) 108, 483, 1072
BstDEI CTNAG 1 cut(s) 556
BstDSI CCRYGG 4 cut(s) 352, 616, 1032, 1105
BstF5I GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BstFNI CGCG 1 cut(s) 330
BstH2I RGCGCY 1 cut(s) 1077
BstHHI GCGC 1 cut(s) 1076
BstKTI GATC 3 cut(s) 806, 901, 1087
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 3 cut(s) 803, 898, 1084
BstMCI CGRYCG 1 cut(s) 901
BstMWI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
BstNI CCWGG 3 cut(s) 287, 318, 464
BstSCI CCNGG 5 cut(s) 140, 285, 316, 462, 1002
BstSFI CTRYAG 2 cut(s) 849, 871
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 4 cut(s) 224, 518, 562, 613
BstV2I GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BstX2I RGATCY 1 cut(s) 803
BstXI CCANNNNNNTGG 1 cut(s) 61
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 4 cut(s) 352, 616, 1032, 1105
BtsCI GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BtsI GCAGTG 1 cut(s) 463
BtsIMutI CAGTG 3 cut(s) 463, 564, 787
Cac8I GCNNGC 3 cut(s) 108, 483, 1072
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 1076
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 139
CsiI ACCWGGT 1 cut(s) 462
Csp6I GTAC 4 cut(s) 283, 359, 656, 1046
CviAII CATG 5 cut(s) 280, 347, 1033, 1106, 1150
CviQI GTAC 4 cut(s) 283, 359, 656, 1046
DdeI CTNAG 1 cut(s) 556
DpnI GATC 3 cut(s) 805, 900, 1086
DpnII GATC 3 cut(s) 803, 898, 1084
Eam1104I CTCTTC 4 cut(s) 168, 176, 318, 495
EarI CTCTTC 4 cut(s) 168, 176, 318, 495
Eco130I CCWWGG 3 cut(s) 1017, 1032, 1105
Eco31I GGTCTC 1 cut(s) 764
Eco88I CYCGRG 1 cut(s) 1049
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 3 cut(s) 285, 316, 462
EcoT14I CCWWGG 3 cut(s) 1017, 1032, 1105
EcoT22I ATGCAT 1 cut(s) 936
ErhI CCWWGG 3 cut(s) 1017, 1032, 1105
FaeI CATG 5 cut(s) 283, 350, 1036, 1109, 1153
FaqI GGGAC 1 cut(s) 593
FatI CATG 5 cut(s) 279, 346, 1032, 1105, 1149
FauI CCCGC 2 cut(s) 99, 490
Fnu4HI GCNGC 7 cut(s) 7, 10, 213, 507, 576, 585, 627
FokI GGATG 9 cut(s) 95, 205, 260, 539, 598, 809, 986, 1015, 1049
Fsp4HI GCNGC 7 cut(s) 7, 10, 213, 507, 576, 585, 627
FspBI CTAG 1 cut(s) 542
GlaI GCGC 1 cut(s) 1075
GluI GCNGC 7 cut(s) 7, 10, 213, 507, 576, 585, 627
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 1077
HaeIII GGCC 1 cut(s) 140
HapII CCGG 4 cut(s) 142, 588, 630, 1003
HhaI GCGC 1 cut(s) 1076
Hin1II CATG 5 cut(s) 283, 350, 1036, 1109, 1153
Hin6I GCGC 1 cut(s) 1074
HinP1I GCGC 1 cut(s) 1074
HinfI GANTC 4 cut(s) 206, 554, 894, 1007
HpaII CCGG 4 cut(s) 142, 588, 630, 1003
HphI GGTGA 4 cut(s) 238, 407, 632, 903
Hpy166II GTNNAC 1 cut(s) 911
Hpy188III TCNNGA 1 cut(s) 488
Hpy8I GTNNAC 1 cut(s) 911
HpyAV CCTTC 1 cut(s) 424
HpyCH4III ACNGT 2 cut(s) 438, 655
HpyCH4V TGCA 8 cut(s) 24, 38, 530, 647, 862, 934, 1040, 1070
HpyF10VI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
HpyF3I CTNAG 1 cut(s) 556
Hsp92II CATG 5 cut(s) 283, 350, 1036, 1109, 1153
HspAI GCGC 1 cut(s) 1074
Kzo9I GATC 3 cut(s) 803, 898, 1084
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 4 cut(s) 224, 518, 562, 613
LweI GCATC 3 cut(s) 25, 517, 1027
MabI ACCWGGT 1 cut(s) 462
MaeI CTAG 1 cut(s) 542
MaeIII GTNAC 2 cut(s) 458, 787
MalI GATC 3 cut(s) 805, 900, 1086
MbiI CCGCTC 2 cut(s) 106, 485
MboI GATC 3 cut(s) 803, 898, 1084
MfeI CAATTG 1 cut(s) 952
MflI RGATCY 1 cut(s) 803
MluCI AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
MlyI GAGTC 2 cut(s) 200, 548
MmeI TCCRAC 2 cut(s) 289, 407
MnlI CCTC 9 cut(s) 121, 177, 416, 496, 589, 711, 787, 1045, 1087
Mph1103I ATGCAT 1 cut(s) 936
MseI TTAA 3 cut(s) 225, 468, 570
MslI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
MspI CCGG 4 cut(s) 142, 588, 630, 1003
MspR9I CCNGG 5 cut(s) 142, 287, 318, 464, 1004
MunI CAATTG 1 cut(s) 952
MvaI CCWGG 3 cut(s) 287, 318, 464
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
NciI CCSGG 2 cut(s) 142, 1004
NcoI CCATGG 2 cut(s) 1032, 1105
NdeII GATC 3 cut(s) 803, 898, 1084
NlaIII CATG 5 cut(s) 283, 350, 1036, 1109, 1153
NlaIV GGNNCC 2 cut(s) 88, 1125
NmuCI GTSAC 2 cut(s) 458, 787
NsiI ATGCAT 1 cut(s) 936
PaeR7I CTCGAG 1 cut(s) 1049
PciSI GCTCTTC 1 cut(s) 318
PfeI GAWTC 2 cut(s) 894, 1007
PflFI GACNNNGTC 1 cut(s) 821
PflMI CCANNNNNTGG 1 cut(s) 617
PfoI TCCNGGA 1 cut(s) 1002
PkrI GCNGC 7 cut(s) 8, 11, 214, 508, 577, 586, 628
Ple19I CGATCG 1 cut(s) 901
PleI GAGTC 2 cut(s) 200, 548
PpsI GAGTC 2 cut(s) 200, 548
Psp6I CCWGG 3 cut(s) 285, 316, 462
PspGI CCWGG 3 cut(s) 285, 316, 462
PspN4I GGNNCC 2 cut(s) 88, 1125
PspPI GGNCC 1 cut(s) 139
PspXI VCTCGAGB 1 cut(s) 1049
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 821
PvuI CGATCG 1 cut(s) 901
RsaI GTAC 4 cut(s) 284, 360, 657, 1047
RsaNI GTAC 4 cut(s) 283, 359, 656, 1046
RseI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 3 cut(s) 225, 468, 570
SatI GCNGC 7 cut(s) 7, 10, 213, 507, 576, 585, 627
Sau3AI GATC 3 cut(s) 803, 898, 1084
Sau96I GGNCC 1 cut(s) 139
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 2 cut(s) 200, 548
ScrFI CCNGG 5 cut(s) 142, 287, 318, 464, 1004
SexAI ACCWGGT 1 cut(s) 462
SfaNI GCATC 3 cut(s) 25, 517, 1027
SfcI CTRYAG 2 cut(s) 849, 871
Sfr274I CTCGAG 1 cut(s) 1049
SlaI CTCGAG 1 cut(s) 1049
SmiMI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
SmlI CTYRAG 2 cut(s) 200, 1049
SmoI CTYRAG 2 cut(s) 200, 1049
Sse9I AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
SsiI CCGC 4 cut(s) 106, 210, 483, 585
SspI AATATT 2 cut(s) 58, 1095
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 5 cut(s) 140, 285, 316, 462, 1002
StyI CCWWGG 3 cut(s) 1017, 1032, 1105
TaaI ACNGT 2 cut(s) 438, 655
TaqI TCGA 7 cut(s) 75, 126, 421, 897, 1050, 1083, 1157
TasI AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
TatI WGTACW 2 cut(s) 655, 1045
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 2 cut(s) 894, 1007
Tru1I TTAA 3 cut(s) 225, 468, 570
Tru9I TTAA 3 cut(s) 225, 468, 570
TscAI CASTG 3 cut(s) 463, 564, 787
TseFI GTSAC 2 cut(s) 458, 787
TseI GCWGC 6 cut(s) 6, 9, 212, 506, 575, 626
Tsp45I GTSAC 2 cut(s) 458, 787
TspDTI ATGAA 4 cut(s) 524, 650, 992, 1017
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 3 cut(s) 463, 564, 787
Tth111I GACNNNGTC 1 cut(s) 821
Van91I CCANNNNNTGG 1 cut(s) 617
XapI RAATTY 2 cut(s) 131, 1023
XhoI CTCGAG 1 cut(s) 1049
XspI CTAG 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 1047
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.