Rw2G027900

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0007520
Physical Location & Seq
Forward (+)
44428293 .. 44431799
3507 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027900.1

Sequence Viewer

Length: 1101 bp
ATGTTTTGTTGTGGTTCAGAGCGGGCTACAAAGAGCGTCTTCAAGGAGTTTTTGGCCAAGTATGGCAAGACATTGAATAAGGAAAGGGAAGAGAAGAGGGAGATTGGGAAGACACTCAAAGACTCGTCTACCGCCACTGTTGAGGACTATGATCTTCCATTGACTCCTGACCAGTTTATTGAAGAAATCATCCCCATGTATCAACAAAAGTGGAAGCACGTGAAAGCTCTTCCTGGTGCTACCCGTCTTATCAAACATCTCCATGACCATGGAGTACCTACTGCTCTTGCTTCAAATTCCTTGCGCGAATATATAAATGCAAAGATCTCTGTCCATCAAGGTTGGCAGGGATGGTTTTCAGTAATCGTTGGCAGTGACCAGGTTAAAGCGGGCAAGCCCTCTCCAGATTTATTTGAAGAGGCAGCAAGGCAGATGGATGTAGATGCAGCTCACTGCCTTGTGATCGAAGACTCGATGGTTGGCGTTAAAGCTGCCAATGCTGCCGGAATGGAGGTAGTGGCTGTTCCACCCCATGGTGAAGCTGCTGGTTGTTCTGCCCTTGCAAACACTGTGCTCCATTCACTTTTGGAGTTTCGACCTGAGCATTGGGGTCTTCCTCCTTTTGAAGATTGGGTAGATAATGCATTGCCAATTGAAGCAATTAATTTGAGTGGTCTTTATGTTAACGGGTTTGTCATTGAAGACACAGAGGATGGGAAATCTGCTCTGCCTGACCAAGTTTGGGGAGTTTTCTTTGGGTGGGCCGTGGTGGATATGCAGAAGAGCTACAGGGTAGTGGTTGGCATTGGAATGGATCACAATTCCTGCTGTCCTAAGAAAAAGACTCAAATACATCTAGTCAATGGAGGCAATTGCTGTATATCCAATCAGCAAGTGAAACTGCTGCTTGTTGGATACATCTGCGGACTGAATAGCAAGGAAGTTTCATCTCTGGATGCAGAATCACTCGAGGAATGCAAGTCTATTGCAAGTGCTGCATTGGATCTACCAATATTTAGTCAGCATGCTTATGTGCCTCTGTACCCAGATACTTTTTCTCTCGAAGACATGGTTGACACTGATGAGCTAGGACACTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

366

Amino Acids

40.29

Weight (kDa)

5.12

Isoelectric Point (pI)

33.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 8 - 175 1.5e-21 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 72 - 169 5.9e-20 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 130 - 175 4.1e-07 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 533
AccBSI CCGCTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 128
AccII CGCG 1 cut(s) 306
AciI CCGC 4 cut(s) 22, 132, 389, 924
AclWI GGATC 2 cut(s) 822, 1011
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 295
AcvI CACGTG 1 cut(s) 220
AfaI GTAC 2 cut(s) 276, 1043
AfiI CCNNNNNNNGG 2 cut(s) 533, 742
AgsI TTSAA 8 cut(s) 43, 76, 182, 294, 416, 626, 656, 701
AjnI CCWGG 2 cut(s) 232, 378
AluBI AGCT 6 cut(s) 227, 449, 491, 542, 786, 1087
AluI AGCT 6 cut(s) 227, 449, 491, 542, 786, 1087
Alw21I GWGCWC 1 cut(s) 576
AlwI GGATC 2 cut(s) 822, 1011
Ama87I CYCGRG 1 cut(s) 968
AoxI GGCC 2 cut(s) 54, 762
ApeKI GCWGC 7 cut(s) 422, 446, 491, 500, 542, 904, 995
ApoI RAATTY 1 cut(s) 295
AseI ATTAAT 1 cut(s) 663
AspLEI GCGC 1 cut(s) 306
AspS9I GGNCC 1 cut(s) 762
AsuHPI GGTGA 1 cut(s) 548
AvaI CYCGRG 1 cut(s) 968
BalI TGGCCA 1 cut(s) 56
BbrPI CACGTG 1 cut(s) 220
BbsI GAAGAC 6 cut(s) 31, 116, 474, 605, 708, 1071
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 7 cut(s) 434, 458, 478, 487, 529, 891, 982
BccI CCATC 5 cut(s) 342, 345, 427, 469, 707
BceAI ACGGC 1 cut(s) 749
BciT130I CCWGG 2 cut(s) 234, 380
BciVI GTATCC 1 cut(s) 908
BfaI CTAG 2 cut(s) 857, 1088
BfmI CTRYAG 1 cut(s) 787
BfuI GTATCC 1 cut(s) 908
BglII AGATCT 1 cut(s) 324
BisI GCNGC 7 cut(s) 423, 447, 492, 501, 543, 905, 996
BlsI GCNGC 7 cut(s) 424, 448, 493, 502, 544, 906, 997
Bme1390I CCNGG 2 cut(s) 234, 380
BmeT110I CYCGRG 1 cut(s) 968
BmgT120I GGNCC 1 cut(s) 762
BmrFI CCNGG 2 cut(s) 234, 380
BmsI GCATC 2 cut(s) 433, 946
BpiI GAAGAC 6 cut(s) 31, 116, 474, 605, 708, 1071
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 600
BsaAI YACGTR 1 cut(s) 220
BsaJI CCNNGG 3 cut(s) 268, 532, 765
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 742
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 644
BseBI CCWGG 2 cut(s) 234, 380
BseDI CCNNGG 3 cut(s) 268, 532, 765
BseGI GGATG 5 cut(s) 189, 356, 442, 718, 961
BseLI CCNNNNNNNGG 2 cut(s) 533, 742
BseMI GCAATG 1 cut(s) 644
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 7 cut(s) 434, 458, 478, 487, 529, 891, 982
Bsh1236I CGCG 1 cut(s) 306
BshFI GGCC 2 cut(s) 56, 764
BsiHKAI GWGCWC 1 cut(s) 576
BsiHKCI CYCGRG 1 cut(s) 968
BsiSI CCGG 1 cut(s) 504
BslI CCNNNNNNNGG 2 cut(s) 533, 742
BsmI GAATGC 1 cut(s) 980
BsnI GGCC 2 cut(s) 56, 764
BsoBI CYCGRG 1 cut(s) 968
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 5 cut(s) 151, 324, 462, 814, 1003
Bsp19I CCATGG 2 cut(s) 268, 532
BspACI CCGC 4 cut(s) 22, 132, 389, 924
BspANI GGCC 2 cut(s) 56, 764
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 1 cut(s) 306
BspPI GGATC 2 cut(s) 822, 1011
BspQI GCTCTTC 2 cut(s) 234, 776
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 644
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 268, 532, 765
BssMI GATC 5 cut(s) 151, 324, 462, 814, 1003
BssT1I CCWWGG 2 cut(s) 268, 532
Bst2UI CCWGG 2 cut(s) 234, 380
Bst4CI ACNGT 2 cut(s) 139, 571
Bst6I CTCTTC 5 cut(s) 84, 89, 234, 411, 776
BstAPI GCANNNNNTGC 1 cut(s) 995
BstBAI YACGTR 1 cut(s) 220
BstC8I GCNNGC 4 cut(s) 24, 391, 395, 1026
BstDEI CTNAG 2 cut(s) 600, 834
BstDSI CCRYGG 3 cut(s) 268, 532, 765
BstF5I GGATG 5 cut(s) 189, 356, 442, 718, 961
BstFNI CGCG 1 cut(s) 306
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 5 cut(s) 154, 327, 465, 817, 1006
BstMBI GATC 5 cut(s) 151, 324, 462, 814, 1003
BstMWI GCNNNNNNNGC 3 cut(s) 497, 500, 995
BstNI CCWGG 2 cut(s) 234, 380
BstNSI RCATGY 1 cut(s) 1028
BstSCI CCNGG 2 cut(s) 232, 378
BstSFI CTRYAG 1 cut(s) 787
BstUI CGCG 1 cut(s) 306
BstV1I GCAGC 7 cut(s) 434, 458, 478, 487, 529, 891, 982
BstV2I GAAGAC 6 cut(s) 31, 116, 474, 605, 708, 1071
BstX2I RGATCY 2 cut(s) 324, 1003
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 2 cut(s) 324, 1003
BsuI GTATCC 1 cut(s) 908
BsuRI GGCC 2 cut(s) 56, 764
BtgI CCRYGG 3 cut(s) 268, 532, 765
BtsCI GGATG 5 cut(s) 189, 356, 442, 718, 961
BtsI GCAGTG 2 cut(s) 379, 451
BtsIMutI CAGTG 5 cut(s) 135, 379, 451, 567, 1077
Cac8I GCNNGC 4 cut(s) 24, 391, 395, 1026
CfoI GCGC 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 762
CseI GACGC 1 cut(s) 25
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 2 cut(s) 275, 1042
CspCI CAANNNNNGTGG 2 cut(s) 191, 226
CviAII CATG 6 cut(s) 196, 263, 269, 533, 1025, 1069
CviQI GTAC 2 cut(s) 275, 1042
DdeI CTNAG 2 cut(s) 600, 834
DpnI GATC 5 cut(s) 153, 326, 464, 816, 1005
DpnII GATC 5 cut(s) 151, 324, 462, 814, 1003
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 5 cut(s) 84, 89, 234, 411, 776
EarI CTCTTC 5 cut(s) 84, 89, 234, 411, 776
Eco130I CCWWGG 2 cut(s) 268, 532
Eco72I CACGTG 1 cut(s) 220
Eco88I CYCGRG 1 cut(s) 968
EcoRII CCWGG 2 cut(s) 232, 378
EcoT14I CCWWGG 2 cut(s) 268, 532
EcoT22I ATGCAT 1 cut(s) 646
ErhI CCWWGG 2 cut(s) 268, 532
FaeI CATG 6 cut(s) 199, 266, 272, 536, 1028, 1072
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FatI CATG 6 cut(s) 195, 262, 268, 532, 1024, 1068
FauI CCCGC 2 cut(s) 15, 382
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 7 cut(s) 423, 447, 492, 501, 543, 905, 996
FokI GGATG 5 cut(s) 176, 363, 449, 725, 968
Fsp4HI GCNGC 7 cut(s) 423, 447, 492, 501, 543, 905, 996
FspBI CTAG 2 cut(s) 857, 1088
GlaI GCGC 1 cut(s) 305
GluI GCNGC 7 cut(s) 423, 447, 492, 501, 543, 905, 996
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 2 cut(s) 56, 764
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 306
Hin1II CATG 6 cut(s) 199, 266, 272, 536, 1028, 1072
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 2 cut(s) 685, 1075
HindII GTYRAC 2 cut(s) 685, 1075
HinfI GANTC 5 cut(s) 122, 163, 470, 844, 962
HpaI GTTAAC 1 cut(s) 685
HpaII CCGG 1 cut(s) 504
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 3 cut(s) 129, 685, 1075
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 4 cut(s) 167, 404, 953, 1061
Hpy8I GTNNAC 3 cut(s) 129, 685, 1075
HpyCH4III ACNGT 2 cut(s) 139, 571
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 9 cut(s) 320, 446, 563, 644, 778, 959, 978, 989, 998
HpyF10VI GCNNNNNNNGC 3 cut(s) 497, 500, 995
HpyF3I CTNAG 2 cut(s) 600, 834
HpySE526I ACGT 1 cut(s) 219
Hsp92II CATG 6 cut(s) 199, 266, 272, 536, 1028, 1072
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 685
Kzo9I GATC 5 cut(s) 151, 324, 462, 814, 1003
LguI GCTCTTC 2 cut(s) 234, 776
LmnI GCTCC 1 cut(s) 579
Lsp1109I GCAGC 7 cut(s) 434, 458, 478, 487, 529, 891, 982
LweI GCATC 2 cut(s) 433, 946
MabI ACCWGGT 1 cut(s) 378
MaeI CTAG 2 cut(s) 857, 1088
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 1 cut(s) 374
MalI GATC 5 cut(s) 153, 326, 464, 816, 1005
MbiI CCGCTC 1 cut(s) 22
MboI GATC 5 cut(s) 151, 324, 462, 814, 1003
MfeI CAATTG 2 cut(s) 651, 871
MflI RGATCY 2 cut(s) 324, 1003
MhlI GDGCHC 1 cut(s) 576
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 6 cut(s) 295, 651, 660, 664, 820, 871
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 4 cut(s) 116, 157, 464, 838
MmeI TCCRAC 1 cut(s) 892
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 646
MscI TGGCCA 1 cut(s) 56
MseI TTAA 4 cut(s) 384, 486, 663, 684
MslI CAYNNNNRTG 5 cut(s) 194, 261, 267, 809, 1029
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 2 cut(s) 234, 380
MunI CAATTG 2 cut(s) 651, 871
Mva1269I GAATGC 1 cut(s) 980
MvaI CCWGG 2 cut(s) 234, 380
MvnI CGCG 1 cut(s) 306
MwoI GCNNNNNNNGC 3 cut(s) 497, 500, 995
NcoI CCATGG 2 cut(s) 268, 532
NdeII GATC 5 cut(s) 151, 324, 462, 814, 1003
NlaIII CATG 6 cut(s) 199, 266, 272, 536, 1028, 1072
NmuCI GTSAC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 646
NspI RCATGY 1 cut(s) 1028
PaeI GCATGC 1 cut(s) 1028
PaeR7I CTCGAG 1 cut(s) 968
PciSI GCTCTTC 2 cut(s) 234, 776
PctI GAATGC 1 cut(s) 980
PfeI GAWTC 1 cut(s) 962
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 7 cut(s) 424, 448, 493, 502, 544, 906, 997
PleI GAGTC 4 cut(s) 116, 157, 464, 838
PmaCI CACGTG 1 cut(s) 220
PmlI CACGTG 1 cut(s) 220
PpsI GAGTC 4 cut(s) 116, 157, 464, 838
Ppu21I YACGTR 1 cut(s) 220
PshBI ATTAAT 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 232, 378
PspCI CACGTG 1 cut(s) 220
PspGI CCWGG 2 cut(s) 232, 378
PspPI GGNCC 1 cut(s) 762
PspXI VCTCGAGB 1 cut(s) 968
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PsuI RGATCY 2 cut(s) 324, 1003
RsaI GTAC 2 cut(s) 276, 1043
RsaNI GTAC 2 cut(s) 275, 1042
RseI CAYNNNNRTG 5 cut(s) 194, 261, 267, 809, 1029
SapI GCTCTTC 2 cut(s) 234, 776
SaqAI TTAA 4 cut(s) 384, 486, 663, 684
SatI GCNGC 7 cut(s) 423, 447, 492, 501, 543, 905, 996
Sau3AI GATC 5 cut(s) 151, 324, 462, 814, 1003
Sau96I GGNCC 1 cut(s) 762
SchI GAGTC 4 cut(s) 116, 157, 464, 838
ScrFI CCNGG 2 cut(s) 234, 380
SduI GDGCHC 1 cut(s) 576
SexAI ACCWGGT 1 cut(s) 378
SfaNI GCATC 2 cut(s) 433, 946
SfcI CTRYAG 1 cut(s) 787
Sfr274I CTCGAG 1 cut(s) 968
SlaI CTCGAG 1 cut(s) 968
SmiMI CAYNNNNRTG 5 cut(s) 194, 261, 267, 809, 1029
SmlI CTYRAG 1 cut(s) 968
SmoI CTYRAG 1 cut(s) 968
SphI GCATGC 1 cut(s) 1028
Sse9I AATT 6 cut(s) 295, 651, 660, 664, 820, 871
SsiI CCGC 4 cut(s) 22, 132, 389, 924
SspI AATATT 1 cut(s) 1014
SspMI CTAG 2 cut(s) 857, 1088
StyD4I CCNGG 2 cut(s) 232, 378
StyI CCWWGG 2 cut(s) 268, 532
TaaI ACNGT 2 cut(s) 139, 571
TaiI ACGT 1 cut(s) 222
TaqI TCGA 5 cut(s) 465, 473, 595, 969, 1062
TasI AATT 6 cut(s) 295, 651, 660, 664, 820, 871
TfiI GAWTC 1 cut(s) 962
Tru1I TTAA 4 cut(s) 384, 486, 663, 684
Tru9I TTAA 4 cut(s) 384, 486, 663, 684
TscAI CASTG 5 cut(s) 142, 379, 458, 574, 1084
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 7 cut(s) 422, 446, 491, 500, 542, 904, 995
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 936
TspRI CASTG 5 cut(s) 142, 379, 458, 574, 1084
Van91I CCANNNNNTGG 1 cut(s) 533
VspI ATTAAT 1 cut(s) 663
XapI RAATTY 1 cut(s) 295
XceI RCATGY 1 cut(s) 1028
XhoI CTCGAG 1 cut(s) 968
XmiI GTMKAC 1 cut(s) 128
XspI CTAG 2 cut(s) 857, 1088
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.