MD17G1231100.v1.1

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007520
Physical Location & Seq
Forward (+)
27946952 .. 27950456
3505 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1231100.v1.1.491

Sequence Viewer

Length: 1182 bp
ATGAGCTGCCGTGGGTGTGAGTGCAACAATGCAGATGCAGAGAACCCAAAACCCAAGATTTTGGCTGTCATTTTTTATTTGGATGGCACCCTTTTGGACACAGAGTGGGCAACAAGGGGCGTGTTCGAGGAATTCTTGGCCCGGAATGGCAAAGTATTGGACAAGGAAAGGGAGGAGAAGAAGAGGTTGGGGATGACACTAAAGGACTCGGCAGCTTCGGTTGTTAAGGACTATCATCTCCCATTCACCCCTGACGAGTTTGTCCAACAAATCATCCCCATGTATCAGGAAAAGTGGAAGTACGCGAAAGCTCTTCCTGGTGCTAATCGCGTTATCAAACACTTCCATGACCACGGAGTACCTATGGCTCTTGCTTCAAACTCCTTACGTGAATACATAGAAGCAAAAATCTCTCACCATCGAGGTTGGAAGGAACTGTTTTCAGTAATTCTTGGCAGTGACCAAGTTAAAGCGGGGAAGCCCGCTCCAGATTTATTTGAAGAGGCAGCGAAGCAAATGGGTGTGGATGCAGTTCATTGCCTAGTGATTGAAGACTCAGTGGTTGGTGTTAAAGCTGCCAAAGCTGCCGGAATGGAGGTAGTGGCTGTCCCATCCCGTGGTGAAGCTGCCGGTTGTTCTTCCCTTGCAGATACTGTGCTTCATTCTCTTTTGGAGTTTCAGCCCGAACATTGGGGTCTTCCTCAATTTGAAGACTGGGTAGATAAGGCACTGCCGATTGAACCAATTTATTTTAGTGGTCTCTATGCCAATGGGTTTGTCAGTGAAGTCACAGAGGATGGAAGATCTGCTCTCCCCGACCAAGTCTGGGGAGTTTTCTTCGGTTGGGCTGTAGCTGATACGCAGAAGACCTTCAGGGTAGTGGTTGCCATTGGATTGGACTGCAATAGTTCACCTCATAAAAACATTCAAATGCATACAGTTGATGGAAACAATTGCTGTATATCCAATCAGCAAATGAAACTGCTACTTGTTGGCTACATCCGGGAATCAAATACCAAGGAAATTTCATCCATGGATGCAGAAGCACTCGAGGAATGTAAGTTGATTGCCAGCGCTTCGCTCGATCTGCCAATATTTAGTCACCATGGTTGTGTGCCTTTGGTTCCACGATCTTTTTCTGTGGAAGACATGACTTCGAGTGATGAGATGGAACGATACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

394

Amino Acids

43.57

Weight (kDa)

5.24

Isoelectric Point (pI)

37.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 22 - 197 1.2e-23 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 23 - 203 2.2e-26 Haloacid dehalogenase-like hydrolase
HAD PF12710 23 - 153 1.5e-06 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 158 - 205 2.6e-06 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 260
AccB1I GGYRCC 1 cut(s) 86
AccB7I CCANNNNNTGG 1 cut(s) 617
AccBSI CCGCTC 1 cut(s) 485
AccII CGCG 2 cut(s) 305, 330
AciI CCGC 2 cut(s) 473, 483
AcsI RAATTY 2 cut(s) 131, 1023
AcuI CTGAAG 1 cut(s) 856
AdeI CACNNNGTG 1 cut(s) 105
AfaI GTAC 2 cut(s) 302, 360
AfeI AGCGCT 1 cut(s) 1075
AfiI CCNNNNNNNGG 3 cut(s) 617, 690, 826
AgsI TTSAA 6 cut(s) 378, 500, 551, 710, 740, 929
AjnI CCWGG 1 cut(s) 316
AjuI GAANNNNNNNTTGG 2 cut(s) 170, 202
AluBI AGCT 7 cut(s) 6, 215, 311, 575, 584, 626, 854
AluI AGCT 7 cut(s) 6, 215, 311, 575, 584, 626, 854
Alw26I GTCTC 1 cut(s) 764
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 1049
Aor51HI AGCGCT 1 cut(s) 1075
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 6 cut(s) 6, 212, 506, 575, 584, 626
ApoI RAATTY 2 cut(s) 131, 1023
Asp700I GAANNNNTTC 1 cut(s) 869
AspLEI GCGC 1 cut(s) 1076
AspS9I GGNCC 1 cut(s) 139
AsuC2I CCSGG 2 cut(s) 142, 1004
AsuHPI GGTGA 5 cut(s) 238, 407, 632, 903, 1094
AvaI CYCGRG 1 cut(s) 1049
BanI GGYRCC 1 cut(s) 86
BbsI GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BbvI GCAGC 5 cut(s) 224, 518, 562, 571, 613
BccI CCATC 6 cut(s) 77, 426, 619, 791, 938, 1162
BciT130I CCWGG 1 cut(s) 318
BcnI CCSGG 2 cut(s) 142, 1004
BcoDI GTCTC 1 cut(s) 764
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 849
BfoI RGCGCY 1 cut(s) 1077
BglII AGATCT 1 cut(s) 803
BisI GCNGC 6 cut(s) 7, 213, 507, 576, 585, 627
BlsI GCNGC 6 cut(s) 8, 214, 508, 577, 586, 628
Bme1390I CCNGG 3 cut(s) 142, 318, 1004
BmeT110I CYCGRG 1 cut(s) 1049
BmgT120I GGNCC 1 cut(s) 139
BmiI GGNNCC 2 cut(s) 88, 1125
BmrFI CCNGG 3 cut(s) 142, 318, 1004
BmrI ACTGGG 1 cut(s) 724
BmsI GCATC 3 cut(s) 25, 517, 1027
BmuI ACTGGG 1 cut(s) 724
BpiI GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BpmI CTGGAG 1 cut(s) 471
BpuMI CCSGG 2 cut(s) 142, 1004
BsaAI YACGTR 1 cut(s) 389
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 6 cut(s) 10, 352, 616, 1017, 1032, 1105
Bsc4I CCNNNNNNNGG 3 cut(s) 617, 690, 826
Bse118I RCCGGY 1 cut(s) 629
Bse1I ACTGG 1 cut(s) 719
Bse3DI GCAATG 1 cut(s) 535
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 6 cut(s) 10, 352, 616, 1017, 1032, 1105
BseGI GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BseLI CCNNNNNNNGG 3 cut(s) 617, 690, 826
BseMI GCAATG 1 cut(s) 535
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 1 cut(s) 719
BseRI GAGGAG 1 cut(s) 188
BseXI GCAGC 5 cut(s) 224, 518, 562, 571, 613
Bsh1236I CGCG 2 cut(s) 305, 330
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 86
BsiHKCI CYCGRG 1 cut(s) 1049
BsiSI CCGG 4 cut(s) 142, 588, 630, 1003
BslFI GGGAC 1 cut(s) 593
BslI CCNNNNNNNGG 3 cut(s) 617, 690, 826
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 1 cut(s) 593
BsnI GGCC 1 cut(s) 140
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 1049
Bsp143I GATC 3 cut(s) 803, 1084, 1130
Bsp19I CCATGG 2 cut(s) 1032, 1105
BspACI CCGC 2 cut(s) 473, 483
BspANI GGCC 1 cut(s) 140
BspCNI CTCAG 1 cut(s) 569
BspFNI CGCG 2 cut(s) 305, 330
BspLI GGNNCC 2 cut(s) 88, 1125
BspQI GCTCTTC 1 cut(s) 318
BspT107I GGYRCC 1 cut(s) 86
BspTNI GGTCTC 1 cut(s) 764
BsrBI CCGCTC 1 cut(s) 485
BsrDI GCAATG 1 cut(s) 535
BsrFI RCCGGY 1 cut(s) 629
BsrI ACTGG 1 cut(s) 719
BssAI RCCGGY 1 cut(s) 629
BssECI CCNNGG 6 cut(s) 10, 352, 616, 1017, 1032, 1105
BssMI GATC 3 cut(s) 803, 1084, 1130
BssT1I CCWWGG 3 cut(s) 1017, 1032, 1105
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 3 cut(s) 438, 655, 940
Bst6I CTCTTC 3 cut(s) 176, 318, 495
BstBAI YACGTR 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 483, 1072
BstDEI CTNAG 1 cut(s) 556
BstDSI CCRYGG 5 cut(s) 10, 352, 616, 1032, 1105
BstF5I GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BstFNI CGCG 2 cut(s) 305, 330
BstH2I RGCGCY 1 cut(s) 1077
BstHHI GCGC 1 cut(s) 1076
BstKTI GATC 3 cut(s) 806, 1087, 1133
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 3 cut(s) 803, 1084, 1130
BstMWI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
BstNI CCWGG 1 cut(s) 318
BstSCI CCNGG 3 cut(s) 140, 316, 1002
BstSFI CTRYAG 1 cut(s) 849
BstUI CGCG 2 cut(s) 305, 330
BstV1I GCAGC 5 cut(s) 224, 518, 562, 571, 613
BstV2I GAAGAC 5 cut(s) 558, 689, 717, 872, 1152
BstX2I RGATCY 1 cut(s) 803
BstXI CCANNNNNNTGG 2 cut(s) 61, 895
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 5 cut(s) 10, 352, 616, 1032, 1105
BtsCI GGATG 9 cut(s) 88, 198, 273, 532, 611, 802, 999, 1028, 1042
BtsI GCAGTG 2 cut(s) 463, 728
BtsIMutI CAGTG 4 cut(s) 463, 564, 728, 787
Cac8I GCNNGC 2 cut(s) 483, 1072
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 1076
Cfr10I RCCGGY 1 cut(s) 629
Cfr13I GGNCC 1 cut(s) 139
Csp6I GTAC 2 cut(s) 301, 359
CviAII CATG 5 cut(s) 280, 347, 1033, 1106, 1150
CviQI GTAC 2 cut(s) 301, 359
DdeI CTNAG 1 cut(s) 556
DpnI GATC 3 cut(s) 805, 1086, 1132
DpnII GATC 3 cut(s) 803, 1084, 1130
DraIII CACNNNGTG 1 cut(s) 105
DrdI GACNNNNNNGTC 1 cut(s) 260
DseDI GACNNNNNNGTC 1 cut(s) 260
Eam1104I CTCTTC 3 cut(s) 176, 318, 495
EarI CTCTTC 3 cut(s) 176, 318, 495
Eco130I CCWWGG 3 cut(s) 1017, 1032, 1105
Eco31I GGTCTC 1 cut(s) 764
Eco47III AGCGCT 1 cut(s) 1075
Eco57I CTGAAG 1 cut(s) 856
Eco88I CYCGRG 1 cut(s) 1049
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 1017, 1032, 1105
EcoT22I ATGCAT 1 cut(s) 936
ErhI CCWWGG 3 cut(s) 1017, 1032, 1105
FaeI CATG 5 cut(s) 283, 350, 1036, 1109, 1153
FaqI GGGAC 1 cut(s) 593
FatI CATG 5 cut(s) 279, 346, 1032, 1105, 1149
FauI CCCGC 2 cut(s) 466, 490
Fnu4HI GCNGC 6 cut(s) 7, 213, 507, 576, 585, 627
FokI GGATG 9 cut(s) 95, 205, 260, 539, 598, 809, 986, 1015, 1049
Fsp4HI GCNGC 6 cut(s) 7, 213, 507, 576, 585, 627
FspBI CTAG 1 cut(s) 542
GlaI GCGC 1 cut(s) 1075
GluI GCNGC 6 cut(s) 7, 213, 507, 576, 585, 627
GsuI CTGGAG 1 cut(s) 471
HaeII RGCGCY 1 cut(s) 1077
HaeIII GGCC 1 cut(s) 140
HapII CCGG 4 cut(s) 142, 588, 630, 1003
HhaI GCGC 1 cut(s) 1076
Hin1II CATG 5 cut(s) 283, 350, 1036, 1109, 1153
Hin6I GCGC 1 cut(s) 1074
HinP1I GCGC 1 cut(s) 1074
HinfI GANTC 3 cut(s) 206, 554, 1007
HpaII CCGG 4 cut(s) 142, 588, 630, 1003
HphI GGTGA 5 cut(s) 238, 407, 632, 903, 1094
Hpy166II GTNNAC 1 cut(s) 911
Hpy188III TCNNGA 2 cut(s) 287, 488
Hpy8I GTNNAC 1 cut(s) 911
HpyAV CCTTC 2 cut(s) 424, 880
HpyCH4III ACNGT 3 cut(s) 438, 655, 940
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 8 cut(s) 24, 32, 38, 530, 647, 903, 934, 1040
HpyF10VI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
HpyF3I CTNAG 1 cut(s) 556
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 5 cut(s) 283, 350, 1036, 1109, 1153
HspAI GCGC 1 cut(s) 1074
Kzo9I GATC 3 cut(s) 803, 1084, 1130
LguI GCTCTTC 1 cut(s) 318
LmnI GCTCC 1 cut(s) 490
Lsp1109I GCAGC 5 cut(s) 224, 518, 562, 571, 613
LweI GCATC 3 cut(s) 25, 517, 1027
MaeI CTAG 1 cut(s) 542
MaeII ACGT 1 cut(s) 388
MaeIII GTNAC 3 cut(s) 458, 787, 1100
MalI GATC 3 cut(s) 805, 1086, 1132
MbiI CCGCTC 1 cut(s) 485
MboI GATC 3 cut(s) 803, 1084, 1130
MfeI CAATTG 1 cut(s) 952
MflI RGATCY 1 cut(s) 803
MluCI AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
MlyI GAGTC 2 cut(s) 200, 548
MmeI TCCRAC 2 cut(s) 289, 407
Mph1103I ATGCAT 1 cut(s) 936
MroXI GAANNNNTTC 1 cut(s) 869
MseI TTAA 3 cut(s) 225, 468, 570
MslI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
MspI CCGG 4 cut(s) 142, 588, 630, 1003
MspR9I CCNGG 3 cut(s) 142, 318, 1004
MunI CAATTG 1 cut(s) 952
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 2 cut(s) 305, 330
MwoI GCNNNNNNNGC 3 cut(s) 581, 584, 1087
NciI CCSGG 2 cut(s) 142, 1004
NcoI CCATGG 2 cut(s) 1032, 1105
NdeII GATC 3 cut(s) 803, 1084, 1130
NlaIII CATG 5 cut(s) 283, 350, 1036, 1109, 1153
NlaIV GGNNCC 2 cut(s) 88, 1125
NmeAIII GCCGAG 1 cut(s) 188
NmuCI GTSAC 3 cut(s) 458, 787, 1100
NsiI ATGCAT 1 cut(s) 936
PaeR7I CTCGAG 1 cut(s) 1049
PciSI GCTCTTC 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 869
PfeI GAWTC 1 cut(s) 1007
PflFI GACNNNGTC 1 cut(s) 821
PflMI CCANNNNNTGG 1 cut(s) 617
PfoI TCCNGGA 1 cut(s) 1002
PkrI GCNGC 6 cut(s) 8, 214, 508, 577, 586, 628
PleI GAGTC 2 cut(s) 200, 548
PpsI GAGTC 2 cut(s) 200, 548
Ppu21I YACGTR 1 cut(s) 389
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 2 cut(s) 88, 1125
PspPI GGNCC 1 cut(s) 139
PspXI VCTCGAGB 1 cut(s) 1049
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 803
PsyI GACNNNGTC 1 cut(s) 821
RsaI GTAC 2 cut(s) 302, 360
RsaNI GTAC 2 cut(s) 301, 359
RseI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
SapI GCTCTTC 1 cut(s) 318
SaqAI TTAA 3 cut(s) 225, 468, 570
SatI GCNGC 6 cut(s) 7, 213, 507, 576, 585, 627
Sau3AI GATC 3 cut(s) 803, 1084, 1130
Sau96I GGNCC 1 cut(s) 139
SchI GAGTC 2 cut(s) 200, 548
ScrFI CCNGG 3 cut(s) 142, 318, 1004
SfaNI GCATC 3 cut(s) 25, 517, 1027
SfcI CTRYAG 1 cut(s) 849
Sfr274I CTCGAG 1 cut(s) 1049
SlaI CTCGAG 1 cut(s) 1049
SmiMI CAYNNNNRTG 4 cut(s) 278, 345, 929, 1110
SmlI CTYRAG 1 cut(s) 1049
SmoI CTYRAG 1 cut(s) 1049
Sse9I AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
SsiI CCGC 2 cut(s) 473, 483
SspI AATATT 1 cut(s) 1095
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 3 cut(s) 140, 316, 1002
StyI CCWWGG 3 cut(s) 1017, 1032, 1105
TaaI ACNGT 3 cut(s) 438, 655, 940
TaiI ACGT 1 cut(s) 391
TaqI TCGA 5 cut(s) 126, 421, 1050, 1083, 1157
TasI AATT 6 cut(s) 131, 447, 704, 744, 952, 1023
TfiI GAWTC 1 cut(s) 1007
Tru1I TTAA 3 cut(s) 225, 468, 570
Tru9I TTAA 3 cut(s) 225, 468, 570
TscAI CASTG 4 cut(s) 463, 564, 735, 787
TseFI GTSAC 3 cut(s) 458, 787, 1100
TseI GCWGC 6 cut(s) 6, 212, 506, 575, 584, 626
Tsp45I GTSAC 3 cut(s) 458, 787, 1100
TspDTI ATGAA 4 cut(s) 524, 650, 992, 1017
TspGWI ACGGA 1 cut(s) 369
TspRI CASTG 4 cut(s) 463, 564, 735, 787
Tth111I GACNNNGTC 1 cut(s) 821
Van91I CCANNNNNTGG 1 cut(s) 617
XapI RAATTY 2 cut(s) 131, 1023
XhoI CTCGAG 1 cut(s) 1049
XmnI GAANNNNTTC 1 cut(s) 869
XspI CTAG 1 cut(s) 542
Zsp2I ATGCAT 1 cut(s) 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.