Rh2CG326100

Bifunctional riboflavin kinase FMN phosphatase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0007520
Physical Location & Seq
Reverse (-)
42166587 .. 42170409
3823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG326100.1

Sequence Viewer

Length: 801 bp
ATGTTTTGTTGTGGTTCAGAGCGGGCTACAAAGAGCGTCTTCAAGGAGTTTTTGGCCAAGTATGGCAAGACTTTGAATAAGGAAAGGGAAGAGAAGAGGGAGATTGGGAAGACACTCAAAGACTCGTCTACCGCCACTGTTGAGGACTATGATCTTCCATTGACTCCTGACCAGTTTATTGAAGAAATCATCCCCATGTATCAACAAAAGTGGAAGCACGCGAAATCTCTTCCTGGTGCTACCCGTCTTATCAAACATCTCCATTACCATGGAGTACCTACTGCTCTTGCTTCAAATTCCTTGCGCGAATATATAAATGCAAAGATCTCTGTCCATCAAGGTTGGCAGGGATGGTTTTCAGTAATCCTTGGCAGTGACCAGGTTAAAGCGGGCAAGCCCTCTCCAGATTTATTTAAAGAGGCAGCAAGGCAGATGGATGTAGATGCAGCTCACTGCCTTGTGATCGAAGACTCGATGGTTGGCGTTAAAGCTGCCAATGCTGCCGGAATGGAGGTAGTGGCTGTTCCACCCCATGGTGAAGCTGCTGGTTGTTCTGCCCTTGCAAACACTGTGCTCCATTCACTTTTGGAGTTTCGACCTGAGCATTGGGGTCTTCCTCCTTTTGAAGATTGTATATATCTCTTCATACTGTTTGCATTTATTTGGTCATATGCCCTTCCTTCCCTGTTCAATACTTGCTTGTCATTTGGTGCAGGGGTAGATAATGCATTGCCAATTGAAGCAATTAATTTGAGTGGTCTTTATGTTAACGGGTTTGTCATTGAAGACACAGGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00740 ko01100 ko01110 map00740 map01100 map01110
Pfam Domains
Protein Families
HAD_2

Protein Analysis

266

Amino Acids

29.33

Weight (kDa)

5.72

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HAD_2 PF13419 8 - 175 4.6e-20 Haloacid dehalogenase-like hydrolase
Hydrolase PF00702 72 - 169 5.8e-19 haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 130 - 175 3.1e-07 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 533
AccBSI CCGCTC 1 cut(s) 22
AccI GTMKAC 1 cut(s) 128
AccII CGCG 2 cut(s) 221, 306
AciI CCGC 3 cut(s) 22, 132, 389
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 295
AfaI GTAC 1 cut(s) 276
AfiI CCNNNNNNNGG 1 cut(s) 533
AgsI TTSAA 8 cut(s) 43, 76, 182, 294, 626, 691, 740, 785
AjnI CCWGG 2 cut(s) 232, 378
AluBI AGCT 3 cut(s) 449, 491, 542
AluI AGCT 3 cut(s) 449, 491, 542
Alw21I GWGCWC 1 cut(s) 576
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 5 cut(s) 422, 446, 491, 500, 542
ApoI RAATTY 1 cut(s) 295
AseI ATTAAT 1 cut(s) 747
AspLEI GCGC 1 cut(s) 306
AsuHPI GGTGA 1 cut(s) 548
BalI TGGCCA 1 cut(s) 56
BbsI GAAGAC 5 cut(s) 31, 116, 474, 605, 792
Bbv12I GWGCWC 1 cut(s) 576
BbvI GCAGC 5 cut(s) 434, 458, 478, 487, 529
BccI CCATC 4 cut(s) 342, 345, 427, 469
BciT130I CCWGG 2 cut(s) 234, 380
BglII AGATCT 1 cut(s) 324
BisI GCNGC 5 cut(s) 423, 447, 492, 501, 543
BlsI GCNGC 5 cut(s) 424, 448, 493, 502, 544
Bme1390I CCNGG 2 cut(s) 234, 380
BmrFI CCNGG 2 cut(s) 234, 380
BmsI GCATC 1 cut(s) 433
BpiI GAAGAC 5 cut(s) 31, 116, 474, 605, 792
BpmI CTGGAG 1 cut(s) 387
Bpu10I CCTNAGC 1 cut(s) 600
BsaJI CCNNGG 3 cut(s) 268, 367, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 533
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 728
BseBI CCWGG 2 cut(s) 234, 380
BseDI CCNNGG 3 cut(s) 268, 367, 532
BseGI GGATG 3 cut(s) 189, 356, 442
BseLI CCNNNNNNNGG 1 cut(s) 533
BseMI GCAATG 1 cut(s) 728
BseMII CTCAG 1 cut(s) 591
BseNI ACTGG 1 cut(s) 172
BseXI GCAGC 5 cut(s) 434, 458, 478, 487, 529
BsgI GTGCAG 1 cut(s) 732
Bsh1236I CGCG 2 cut(s) 221, 306
BshFI GGCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 576
BsiSI CCGG 1 cut(s) 504
BslI CCNNNNNNNGG 1 cut(s) 533
BsnI GGCC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 576
Bsp143I GATC 3 cut(s) 151, 324, 462
Bsp19I CCATGG 2 cut(s) 268, 532
BspACI CCGC 3 cut(s) 22, 132, 389
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 1 cut(s) 592
BspFNI CGCG 2 cut(s) 221, 306
BsrBI CCGCTC 1 cut(s) 22
BsrDI GCAATG 1 cut(s) 728
BsrI ACTGG 1 cut(s) 172
BssECI CCNNGG 3 cut(s) 268, 367, 532
BssMI GATC 3 cut(s) 151, 324, 462
BssT1I CCWWGG 3 cut(s) 268, 367, 532
Bst2UI CCWGG 2 cut(s) 234, 380
Bst4CI ACNGT 3 cut(s) 139, 571, 651
Bst6I CTCTTC 4 cut(s) 84, 89, 234, 647
BstC8I GCNNGC 4 cut(s) 24, 219, 391, 395
BstDEI CTNAG 1 cut(s) 600
BstDSI CCRYGG 2 cut(s) 268, 532
BstF5I GGATG 3 cut(s) 189, 356, 442
BstFNI CGCG 2 cut(s) 221, 306
BstHHI GCGC 1 cut(s) 306
BstKTI GATC 3 cut(s) 154, 327, 465
BstMBI GATC 3 cut(s) 151, 324, 462
BstMWI GCNNNNNNNGC 2 cut(s) 497, 500
BstNI CCWGG 2 cut(s) 234, 380
BstSCI CCNGG 2 cut(s) 232, 378
BstUI CGCG 2 cut(s) 221, 306
BstV1I GCAGC 5 cut(s) 434, 458, 478, 487, 529
BstV2I GAAGAC 5 cut(s) 31, 116, 474, 605, 792
BstX2I RGATCY 1 cut(s) 324
BstXI CCANNNNNNTGG 1 cut(s) 269
BstYI RGATCY 1 cut(s) 324
BsuRI GGCC 1 cut(s) 56
BtgI CCRYGG 2 cut(s) 268, 532
BtsCI GGATG 3 cut(s) 189, 356, 442
BtsI GCAGTG 2 cut(s) 379, 451
BtsIMutI CAGTG 4 cut(s) 135, 379, 451, 567
Cac8I GCNNGC 4 cut(s) 24, 219, 391, 395
CfoI GCGC 1 cut(s) 306
CseI GACGC 1 cut(s) 25
CsiI ACCWGGT 1 cut(s) 378
Csp6I GTAC 1 cut(s) 275
CspCI CAANNNNNGTGG 2 cut(s) 191, 226
CviAII CATG 3 cut(s) 196, 269, 533
CviJI RGCY 7 cut(s) 26, 56, 397, 449, 491, 521, 542
CviKI_1 RGCY 7 cut(s) 26, 56, 397, 449, 491, 521, 542
CviQI GTAC 1 cut(s) 275
DdeI CTNAG 1 cut(s) 600
DpnI GATC 3 cut(s) 153, 326, 464
DpnII GATC 3 cut(s) 151, 324, 462
DraI TTTAAA 1 cut(s) 415
EaeI YGGCCR 1 cut(s) 54
Eam1104I CTCTTC 4 cut(s) 84, 89, 234, 647
EarI CTCTTC 4 cut(s) 84, 89, 234, 647
Eco130I CCWWGG 3 cut(s) 268, 367, 532
EcoRII CCWGG 2 cut(s) 232, 378
EcoT14I CCWWGG 3 cut(s) 268, 367, 532
EcoT22I ATGCAT 1 cut(s) 730
ErhI CCWWGG 3 cut(s) 268, 367, 532
FaeI CATG 3 cut(s) 199, 272, 536
FalI AAGNNNNNCTT 2 cut(s) 23, 55
FatI CATG 3 cut(s) 195, 268, 532
FauI CCCGC 2 cut(s) 15, 382
FauNDI CATATG 1 cut(s) 670
FblI GTMKAC 1 cut(s) 128
Fnu4HI GCNGC 5 cut(s) 423, 447, 492, 501, 543
FokI GGATG 3 cut(s) 176, 363, 449
Fsp4HI GCNGC 5 cut(s) 423, 447, 492, 501, 543
GlaI GCGC 1 cut(s) 305
GluI GCNGC 5 cut(s) 423, 447, 492, 501, 543
GsuI CTGGAG 1 cut(s) 387
HaeIII GGCC 1 cut(s) 56
HapII CCGG 1 cut(s) 504
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 306
Hin1II CATG 3 cut(s) 199, 272, 536
Hin6I GCGC 1 cut(s) 304
HinP1I GCGC 1 cut(s) 304
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 3 cut(s) 122, 163, 470
HpaI GTTAAC 1 cut(s) 769
HpaII CCGG 1 cut(s) 504
HphI GGTGA 1 cut(s) 548
Hpy166II GTNNAC 2 cut(s) 129, 769
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 2 cut(s) 167, 404
Hpy8I GTNNAC 2 cut(s) 129, 769
HpyAV CCTTC 2 cut(s) 686, 690
HpyCH4III ACNGT 3 cut(s) 139, 571, 651
HpyCH4V TGCA 6 cut(s) 320, 446, 563, 656, 713, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 497, 500
HpyF3I CTNAG 1 cut(s) 600
Hsp92II CATG 3 cut(s) 199, 272, 536
HspAI GCGC 1 cut(s) 304
KspAI GTTAAC 1 cut(s) 769
Kzo9I GATC 3 cut(s) 151, 324, 462
LmnI GCTCC 1 cut(s) 579
Lsp1109I GCAGC 5 cut(s) 434, 458, 478, 487, 529
LweI GCATC 1 cut(s) 433
MabI ACCWGGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 374
MalI GATC 3 cut(s) 153, 326, 464
MbiI CCGCTC 1 cut(s) 22
MboI GATC 3 cut(s) 151, 324, 462
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 324
MhlI GDGCHC 1 cut(s) 576
MlsI TGGCCA 1 cut(s) 56
MluCI AATT 4 cut(s) 295, 735, 744, 748
MluNI TGGCCA 1 cut(s) 56
MlyI GAGTC 3 cut(s) 116, 157, 464
MnlI CCTC 6 cut(s) 90, 136, 409, 412, 505, 627
Mox20I TGGCCA 1 cut(s) 56
Mph1103I ATGCAT 1 cut(s) 730
MscI TGGCCA 1 cut(s) 56
MseI TTAA 5 cut(s) 384, 414, 486, 747, 768
MslI CAYNNNNRTG 2 cut(s) 194, 267
Msp20I TGGCCA 1 cut(s) 56
MspI CCGG 1 cut(s) 504
MspR9I CCNGG 2 cut(s) 234, 380
MunI CAATTG 1 cut(s) 735
MvaI CCWGG 2 cut(s) 234, 380
MvnI CGCG 2 cut(s) 221, 306
MwoI GCNNNNNNNGC 2 cut(s) 497, 500
NcoI CCATGG 2 cut(s) 268, 532
NdeI CATATG 1 cut(s) 670
NdeII GATC 3 cut(s) 151, 324, 462
NlaIII CATG 3 cut(s) 199, 272, 536
NmuCI GTSAC 1 cut(s) 374
NsiI ATGCAT 1 cut(s) 730
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 5 cut(s) 424, 448, 493, 502, 544
PleI GAGTC 3 cut(s) 116, 157, 464
PpsI GAGTC 3 cut(s) 116, 157, 464
PshBI ATTAAT 1 cut(s) 747
Psp6I CCWGG 2 cut(s) 232, 378
PspGI CCWGG 2 cut(s) 232, 378
PsrI GAACNNNNNNTAC 2 cut(s) 507, 539
PsuI RGATCY 1 cut(s) 324
RsaI GTAC 1 cut(s) 276
RsaNI GTAC 1 cut(s) 275
RseI CAYNNNNRTG 2 cut(s) 194, 267
SaqAI TTAA 5 cut(s) 384, 414, 486, 747, 768
SatI GCNGC 5 cut(s) 423, 447, 492, 501, 543
Sau3AI GATC 3 cut(s) 151, 324, 462
SchI GAGTC 3 cut(s) 116, 157, 464
ScrFI CCNGG 2 cut(s) 234, 380
SduI GDGCHC 1 cut(s) 576
SetI ASST 9 cut(s) 280, 343, 384, 451, 493, 516, 544, 601, 796
SexAI ACCWGGT 1 cut(s) 378
SfaNI GCATC 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 194, 267
Sse9I AATT 4 cut(s) 295, 735, 744, 748
SsiI CCGC 3 cut(s) 22, 132, 389
StyD4I CCNGG 2 cut(s) 232, 378
StyI CCWWGG 3 cut(s) 268, 367, 532
TaaI ACNGT 3 cut(s) 139, 571, 651
TaqI TCGA 3 cut(s) 465, 473, 595
TasI AATT 4 cut(s) 295, 735, 744, 748
Tru1I TTAA 5 cut(s) 384, 414, 486, 747, 768
Tru9I TTAA 5 cut(s) 384, 414, 486, 747, 768
TscAI CASTG 4 cut(s) 142, 379, 458, 574
TseFI GTSAC 1 cut(s) 374
TseI GCWGC 5 cut(s) 422, 446, 491, 500, 542
Tsp45I GTSAC 1 cut(s) 374
TspDTI ATGAA 1 cut(s) 634
TspRI CASTG 4 cut(s) 142, 379, 458, 574
Van91I CCANNNNNTGG 1 cut(s) 533
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 1 cut(s) 295
XmiI GTMKAC 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.