MD00G1202600.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0010019
Physical Location & Seq
Reverse (-)
48326830 .. 48328342
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1202600.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTCTCCTGTAACAACTCCTATGCCGTTTGTCCTGCTCATTTTCCTCACCCTTCCGTTTCTCAGCCACACAGCACCTCAAGCCAGCATCTGCAGAACTTCTTGCGGCAACATTCCCATCAACTACCCTTTTGGAATCGATGAAGGCTGTGGGAGCCCTTACTACAAAAACATCCTCGTATGCTCCGAATCCTCTAAACTCGAACTCCGAACACCTTCAGGAAGATATCCCGTTAAATCCATAAGCTACTCCGACCCTCACATTCTTCTCACCGATCCTTTCATGTGGAATTGCCTTGACGGTGACGCATTCCGAGTGACAAGGCCTTTTAGCCTCGACACCAGCACACATTTATCTCTCTCCCCTCAAAACGACTACCTTTTCTTTAATTGTAGTGAACAAAATGTGATCATTGAGCCAAAGCCTGTGTTTTGTGAGAGGTTTCCTGAGCGGTGTGACTCGTCGTGTGACAGTTCTAGTTATCTTTGCAGGCACTTGCCTGAGTGTGCCTCGGCGTTAAGGACAAGTTCTTGCTGCTCTTACTATCCCAAAGCCACTGAGTCTCTGAGGTTAATGTTGAAGTATTGTCCTACTTATACTAGTGTGTATTGGAGAAACACTGGTGAAAACGAACCGGATGATCAAGTCCCCGAATATGGAATTCGCGTCGATTTCGATATTCCGGTGACCACCCGTTGCCTTCAGTGTCAGGATACATCTAAAGGAGGCGGGACATGTGGATTCGACACAAAAACACATAACTTCATGTGCTTGTGTGACAGAGGAAATGTCACTACTTATTGTAAAGATCATAGCTTTTCTCAGCATAGGAGGAATGGAGTACTTGCAGGGACGATTAGTGGAGTGACAGTTGCAGGAGCCTTTGGAGTTGGAGCTGGAATTTGGTACATGAAGAAAGTGAGAGCTAAAGCACCAGTAACATGTGGGGTTCAGAGCAATGAGAATAGGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

326

Amino Acids

36.23

Weight (kDa)

6.97

Isoelectric Point (pI)

55.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 91 3.2e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 451
AccII CGCG 1 cut(s) 666
AciI CCGC 3 cut(s) 105, 451, 729
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 2 cut(s) 660, 900
AcuI CTGAAG 2 cut(s) 201, 686
AfaI GTAC 2 cut(s) 843, 908
AfiI CCNNNNNNNGG 1 cut(s) 656
AflIII ACRYGT 2 cut(s) 734, 941
AgsI TTSAA 1 cut(s) 580
AhlI ACTAGT 1 cut(s) 599
AluBI AGCT 4 cut(s) 246, 816, 896, 926
AluI AGCT 4 cut(s) 246, 816, 896, 926
Alw26I GTCTC 2 cut(s) 9, 567
AlwI GGATC 1 cut(s) 269
AlwNI CAGNNNCTG 1 cut(s) 90
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 534
ApoI RAATTY 2 cut(s) 660, 900
Asp700I GAANNNNTTC 1 cut(s) 214
AsuHPI GGTGA 5 cut(s) 40, 262, 314, 635, 697
BanII GRGCYC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 521
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 10
BciVI GTATCC 1 cut(s) 706
BclI TGATCA 2 cut(s) 408, 640
BcoDI GTCTC 2 cut(s) 9, 567
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 2 cut(s) 477, 600
BfmI CTRYAG 1 cut(s) 91
BfuI GTATCC 1 cut(s) 706
BisI GCNGC 2 cut(s) 106, 535
BlsI GCNGC 2 cut(s) 107, 536
BmcAI AGTACT 1 cut(s) 843
BmiI GGNNCC 2 cut(s) 155, 880
BmsI GCATC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 494, 526
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 63
Bsa29I ATCGAT 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 510
BsaWI WCCGGW 2 cut(s) 634, 682
BsaXI ACNNNNNCTCC 4 cut(s) 189, 219, 855, 885
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse1I ACTGG 2 cut(s) 625, 935
Bse3DI GCAATG 1 cut(s) 964
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 2 cut(s) 171, 643
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 1 cut(s) 964
BseMII CTCAG 6 cut(s) 76, 438, 492, 549, 557, 836
BseNI ACTGG 2 cut(s) 625, 935
BseXI GCAGC 1 cut(s) 521
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 1 cut(s) 325
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 2 cut(s) 635, 683
BslFI GGGAC 3 cut(s) 632, 745, 865
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 2 cut(s) 9, 567
BsmFI GGGAC 3 cut(s) 632, 745, 865
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 4 cut(s) 274, 408, 640, 808
BspACI CCGC 3 cut(s) 105, 451, 729
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 6 cut(s) 75, 439, 493, 550, 558, 835
BspDI ATCGAT 1 cut(s) 138
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 2 cut(s) 155, 880
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 964
BsrI ACTGG 2 cut(s) 625, 935
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 4 cut(s) 274, 408, 640, 808
Bst4CI ACNGT 3 cut(s) 302, 473, 871
BstC8I GCNNGC 2 cut(s) 85, 491
BstDEI CTNAG 6 cut(s) 62, 447, 501, 558, 566, 822
BstEII GGTNACC 1 cut(s) 685
BstF5I GGATG 2 cut(s) 171, 643
BstFNI CGCG 1 cut(s) 666
BstKTI GATC 4 cut(s) 277, 411, 643, 811
BstMAI GTCTC 2 cut(s) 9, 567
BstMBI GATC 4 cut(s) 274, 408, 640, 808
BstMWI GCNNNNNNNGC 2 cut(s) 80, 153
BstNSI RCATGY 2 cut(s) 738, 945
BstPI GGTNACC 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 91
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 138
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 1 cut(s) 325
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 2 cut(s) 171, 643
BtsIMutI CAGTG 3 cut(s) 555, 618, 710
Cac8I GCNNGC 2 cut(s) 85, 491
CaiI CAGNNNCTG 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 2 cut(s) 314, 655
Csp6I GTAC 2 cut(s) 842, 907
CviAII CATG 5 cut(s) 283, 735, 766, 910, 942
CviQI GTAC 2 cut(s) 842, 907
DdeI CTNAG 6 cut(s) 62, 447, 501, 558, 566, 822
DpnI GATC 4 cut(s) 276, 410, 642, 810
DpnII GATC 4 cut(s) 274, 408, 640, 808
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 158
Eco32I GATATC 1 cut(s) 227
Eco57I CTGAAG 2 cut(s) 201, 686
Eco91I GGTNACC 1 cut(s) 685
EcoO65I GGTNACC 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 660
EcoRV GATATC 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 158
FaeI CATG 5 cut(s) 286, 738, 769, 913, 945
FaqI GGGAC 3 cut(s) 632, 745, 865
FatI CATG 5 cut(s) 282, 734, 765, 909, 941
FauI CCCGC 1 cut(s) 722
FbaI TGATCA 2 cut(s) 408, 640
Fnu4HI GCNGC 2 cut(s) 106, 535
FokI GGATG 2 cut(s) 158, 650
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 2 cut(s) 106, 535
FspBI CTAG 2 cut(s) 477, 600
GluI GCNGC 2 cut(s) 106, 535
HaeIII GGCC 1 cut(s) 325
HapII CCGG 2 cut(s) 635, 683
HgaI GACGC 2 cut(s) 314, 655
Hin1II CATG 5 cut(s) 286, 738, 769, 913, 945
HinfI GANTC 5 cut(s) 135, 188, 458, 560, 741
HpaII CCGG 2 cut(s) 635, 683
HphI GGTGA 5 cut(s) 40, 262, 314, 635, 697
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 6 cut(s) 187, 209, 253, 314, 567, 954
Hpy188III TCNNGA 3 cut(s) 219, 446, 710
Hpy8I GTNNAC 1 cut(s) 398
Hpy99I CGWCG 2 cut(s) 466, 671
HpyAV CCTTC 4 cut(s) 62, 137, 225, 710
HpyCH4III ACNGT 3 cut(s) 302, 473, 871
HpyCH4V TGCA 4 cut(s) 93, 489, 848, 875
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 153
HpyF3I CTNAG 6 cut(s) 62, 447, 501, 558, 566, 822
Hsp92II CATG 5 cut(s) 286, 738, 769, 913, 945
Ksp22I TGATCA 2 cut(s) 408, 640
Kzo9I GATC 4 cut(s) 274, 408, 640, 808
LmnI GCTCC 4 cut(s) 153, 188, 878, 893
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 96
MaeI CTAG 2 cut(s) 477, 600
MalI GATC 4 cut(s) 276, 410, 642, 810
MbiI CCGCTC 1 cut(s) 451
MboI GATC 4 cut(s) 274, 408, 640, 808
MboII GAAGA 3 cut(s) 234, 257, 925
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 4 cut(s) 289, 388, 660, 900
MlyI GAGTC 2 cut(s) 452, 569
MmeI TCCRAC 2 cut(s) 276, 871
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 4 cut(s) 234, 387, 518, 572
MspI CCGG 2 cut(s) 635, 683
Mva1269I GAATGC 1 cut(s) 308
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 2 cut(s) 80, 153
NdeII GATC 4 cut(s) 274, 408, 640, 808
NlaIII CATG 5 cut(s) 286, 738, 769, 913, 945
NlaIV GGNNCC 2 cut(s) 155, 880
NmeAIII GCCGAG 1 cut(s) 491
NmuCI GTSAC 8 cut(s) 302, 316, 455, 467, 685, 776, 790, 865
NspI RCATGY 2 cut(s) 738, 945
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 2 cut(s) 734, 941
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 135, 188, 741
PkrI GCNGC 2 cut(s) 107, 536
PleI GAGTC 2 cut(s) 452, 568
PpsI GAGTC 2 cut(s) 452, 568
PscI ACATGT 2 cut(s) 734, 941
PspEI GGTNACC 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 155, 880
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 90
RsaI GTAC 2 cut(s) 843, 908
RsaNI GTAC 2 cut(s) 842, 907
SaqAI TTAA 4 cut(s) 234, 387, 518, 572
SatI GCNGC 2 cut(s) 106, 535
Sau3AI GATC 4 cut(s) 274, 408, 640, 808
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 2 cut(s) 452, 569
SduI GDGCHC 1 cut(s) 158
SetI ASST 9 cut(s) 79, 217, 248, 381, 443, 572, 818, 898, 928
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 4 cut(s) 289, 388, 660, 900
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 3 cut(s) 105, 451, 729
SspMI CTAG 2 cut(s) 477, 600
StuI AGGCCT 1 cut(s) 325
TaaI ACNGT 3 cut(s) 302, 473, 871
TaqI TCGA 6 cut(s) 138, 201, 336, 669, 675, 744
TasI AATT 4 cut(s) 289, 388, 660, 900
TatI WGTACW 1 cut(s) 841
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 3 cut(s) 135, 188, 741
Tru1I TTAA 4 cut(s) 234, 387, 518, 572
Tru9I TTAA 4 cut(s) 234, 387, 518, 572
TscAI CASTG 3 cut(s) 562, 625, 710
TseFI GTSAC 8 cut(s) 302, 316, 455, 467, 685, 776, 790, 865
TseI GCWGC 1 cut(s) 534
Tsp45I GTSAC 8 cut(s) 302, 316, 455, 467, 685, 776, 790, 865
TspDTI ATGAA 4 cut(s) 156, 271, 754, 926
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 3 cut(s) 562, 625, 710
XapI RAATTY 2 cut(s) 660, 900
XceI RCATGY 2 cut(s) 738, 945
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 2 cut(s) 477, 600
ZrmI AGTACT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.