MD10G1324600.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010019
Physical Location & Seq
Forward (+)
40564280 .. 40565016
737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1324600.v1.1.491

Sequence Viewer

Length: 642 bp
ATGTCTCCTGTAACAACTCCTATGCCGTTTGTCCTGCTCATTTTCCTCACCCTTCCGTTTCTCAGCCACACAGCACCTCAAGCCAGCATCTGCAGAACTTCTTGCGGCAACATTCCCATCAACTACCCTTTTGGAATCGATGAAGGCTGTGGGAGCCCTTACTACAAAAACATCCTCGTATGCTCCGAATCCTCTAAACTCGAACTCCGAACACCTTCAGGAAGATATCCCGTTAAATCCATAAGCTACTCCGACCCTCACATTCTTCTCACCGATCCTTTCATGTGGAATTGCCTTGACGGTGACGCATTCCGAGTGACAAGGCCTTTTAGCCTCGACACCAGCACACATTTATCTCTCTCCCCTCAAAACGACTACCTTTTCTTTAATTGTAGTGAACAAAATGTGATCATTGAGCCAAAGCCTGTGTTTTGTGAGAGGTTTCCTGAGCGGTGTGACTCGTCGTGTGACAGTTCTAGTTATCTTTGCAGGCACTTGCCTGAGTGTGCCTCGGCGTTAAGGACAAGTTCTTGCTGCTCTTACTATCCCAAAGCCACTGAGTCTCTGAGGTTAATGTTGAAGTATTGTCCTACTTATACTAGTGTGTATTGGAGAAACACTGGTGAAAACGAAACCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

214

Amino Acids

24.02

Weight (kDa)

5.79

Isoelectric Point (pI)

69.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 91 1.5e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 451
AciI CCGC 2 cut(s) 105, 451
AclWI GGATC 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 201
AgsI TTSAA 1 cut(s) 580
AhlI ACTAGT 1 cut(s) 599
AluBI AGCT 1 cut(s) 246
AluI AGCT 1 cut(s) 246
Alw26I GTCTC 2 cut(s) 9, 567
AlwI GGATC 1 cut(s) 269
AlwNI CAGNNNCTG 1 cut(s) 90
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 1 cut(s) 534
Asp700I GAANNNNTTC 1 cut(s) 214
AsuHPI GGTGA 4 cut(s) 40, 262, 314, 635
BanII GRGCYC 1 cut(s) 158
BbvI GCAGC 1 cut(s) 521
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 10
BclI TGATCA 1 cut(s) 408
BcoDI GTCTC 2 cut(s) 9, 567
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 2 cut(s) 477, 600
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 2 cut(s) 106, 535
BlsI GCNGC 2 cut(s) 107, 536
BmiI GGNNCC 1 cut(s) 155
BmsI GCATC 1 cut(s) 96
BplI GAGNNNNNCTC 2 cut(s) 494, 526
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 1 cut(s) 63
Bsa29I ATCGAT 1 cut(s) 138
BsaJI CCNNGG 1 cut(s) 510
BsaWI WCCGGW 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bse1I ACTGG 1 cut(s) 625
BseCI ATCGAT 1 cut(s) 138
BseDI CCNNGG 1 cut(s) 510
BseGI GGATG 1 cut(s) 171
BseMII CTCAG 5 cut(s) 76, 438, 492, 549, 557
BseNI ACTGG 1 cut(s) 625
BseXI GCAGC 1 cut(s) 521
BshFI GGCC 1 cut(s) 325
BshVI ATCGAT 1 cut(s) 138
BsiSI CCGG 1 cut(s) 636
BsmAI GTCTC 2 cut(s) 9, 567
BsmI GAATGC 1 cut(s) 308
BsnI GGCC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 158
Bsp143I GATC 2 cut(s) 274, 408
BspACI CCGC 2 cut(s) 105, 451
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 5 cut(s) 75, 439, 493, 550, 558
BspDI ATCGAT 1 cut(s) 138
BspLI GGNNCC 1 cut(s) 155
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 269
BsrBI CCGCTC 1 cut(s) 451
BsrI ACTGG 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 510
BssMI GATC 2 cut(s) 274, 408
Bst4CI ACNGT 2 cut(s) 302, 473
BstC8I GCNNGC 2 cut(s) 85, 491
BstDEI CTNAG 5 cut(s) 62, 447, 501, 558, 566
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 2 cut(s) 277, 411
BstMAI GTCTC 2 cut(s) 9, 567
BstMBI GATC 2 cut(s) 274, 408
BstMWI GCNNNNNNNGC 2 cut(s) 80, 153
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 1 cut(s) 521
Bsu15I ATCGAT 1 cut(s) 138
BsuRI GGCC 1 cut(s) 325
BsuTUI ATCGAT 1 cut(s) 138
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 2 cut(s) 555, 618
Cac8I GCNNGC 2 cut(s) 85, 491
CaiI CAGNNNCTG 1 cut(s) 90
ClaI ATCGAT 1 cut(s) 138
CseI GACGC 1 cut(s) 314
CviAII CATG 1 cut(s) 283
DdeI CTNAG 5 cut(s) 62, 447, 501, 558, 566
DpnI GATC 2 cut(s) 276, 410
DpnII GATC 2 cut(s) 274, 408
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 158
Eco32I GATATC 1 cut(s) 227
Eco57I CTGAAG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 227
EcoT38I GRGCYC 1 cut(s) 158
FaeI CATG 1 cut(s) 286
FaiI YATR 5 cut(s) 23, 181, 242, 284, 597
FatI CATG 1 cut(s) 282
FbaI TGATCA 1 cut(s) 408
Fnu4HI GCNGC 2 cut(s) 106, 535
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 158
Fsp4HI GCNGC 2 cut(s) 106, 535
FspBI CTAG 2 cut(s) 477, 600
GluI GCNGC 2 cut(s) 106, 535
HaeIII GGCC 1 cut(s) 325
HapII CCGG 1 cut(s) 636
HgaI GACGC 1 cut(s) 314
Hin1II CATG 1 cut(s) 286
HinfI GANTC 4 cut(s) 135, 188, 458, 560
HpaII CCGG 1 cut(s) 636
HphI GGTGA 4 cut(s) 40, 262, 314, 635
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 5 cut(s) 187, 209, 253, 314, 567
Hpy188III TCNNGA 2 cut(s) 219, 446
Hpy8I GTNNAC 1 cut(s) 398
Hpy99I CGWCG 1 cut(s) 466
HpyAV CCTTC 3 cut(s) 62, 137, 225
HpyCH4III ACNGT 2 cut(s) 302, 473
HpyCH4V TGCA 2 cut(s) 93, 489
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 153
HpyF3I CTNAG 5 cut(s) 62, 447, 501, 558, 566
Hsp92II CATG 1 cut(s) 286
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 2 cut(s) 274, 408
LmnI GCTCC 2 cut(s) 153, 188
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 96
MaeI CTAG 2 cut(s) 477, 600
MaeIII GTNAC 5 cut(s) 10, 302, 316, 455, 467
MalI GATC 2 cut(s) 276, 410
MbiI CCGCTC 1 cut(s) 451
MboI GATC 2 cut(s) 274, 408
MboII GAAGA 2 cut(s) 234, 257
MhlI GDGCHC 1 cut(s) 158
MluCI AATT 2 cut(s) 289, 388
MlyI GAGTC 2 cut(s) 452, 569
MmeI TCCRAC 1 cut(s) 276
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 4 cut(s) 234, 387, 518, 572
MspI CCGG 1 cut(s) 636
Mva1269I GAATGC 1 cut(s) 308
MwoI GCNNNNNNNGC 2 cut(s) 80, 153
NdeII GATC 2 cut(s) 274, 408
NlaIII CATG 1 cut(s) 286
NlaIV GGNNCC 1 cut(s) 155
NmeAIII GCCGAG 1 cut(s) 491
NmuCI GTSAC 4 cut(s) 302, 316, 455, 467
PceI AGGCCT 1 cut(s) 325
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PctI GAATGC 1 cut(s) 308
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 135, 188
PkrI GCNGC 2 cut(s) 107, 536
PleI GAGTC 2 cut(s) 452, 568
PpsI GAGTC 2 cut(s) 452, 568
PspN4I GGNNCC 1 cut(s) 155
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 90
SaqAI TTAA 4 cut(s) 234, 387, 518, 572
SatI GCNGC 2 cut(s) 106, 535
Sau3AI GATC 2 cut(s) 274, 408
SchI GAGTC 2 cut(s) 452, 569
SduI GDGCHC 1 cut(s) 158
SetI ASST 6 cut(s) 79, 217, 248, 381, 443, 572
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 2 cut(s) 289, 388
SseBI AGGCCT 1 cut(s) 325
SsiI CCGC 2 cut(s) 105, 451
SspMI CTAG 2 cut(s) 477, 600
StuI AGGCCT 1 cut(s) 325
TaaI ACNGT 2 cut(s) 302, 473
TaqI TCGA 3 cut(s) 138, 201, 336
TasI AATT 2 cut(s) 289, 388
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 2 cut(s) 135, 188
Tru1I TTAA 4 cut(s) 234, 387, 518, 572
Tru9I TTAA 4 cut(s) 234, 387, 518, 572
TscAI CASTG 2 cut(s) 562, 625
TseFI GTSAC 4 cut(s) 302, 316, 455, 467
TseI GCWGC 1 cut(s) 534
Tsp45I GTSAC 4 cut(s) 302, 316, 455, 467
TspDTI ATGAA 2 cut(s) 156, 271
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 562, 625
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 2 cut(s) 477, 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.