Rorug04G0396800

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010019
Physical Location & Seq
Forward (+)
56288754 .. 56294987
6234 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0396800.1

Sequence Viewer

Length: 2214 bp
ATGATCCTTTACAGTCCTCTCTTCTCATCCGGCGTTACGCCACGTGGCAAAACCACATCTTCTTCTTCTTCTTCTTCTTATTCTCCGACCCGAGTCAACTCGGACGCTTCACCGGCCGGATCACTCGGGCCGGACTCGTTCTGTCCGAGCGGCTTATGTTCTTGCGGAAGAAGACGCTTCATCGAAGCAGCAGTCACTTCATCTCTATTCCCAATTTGCCCCTCCACCTCCTTCGCTTCCAAGTCGCAGTTCGACGATTATACGGCCACGTTGAGGAAAGTTCACCCTCCTCGACCAGACTGGTATGAAGAGTTGTATGCGTCAGTGTTGAACACCGGCATGCAATCTTACGAAGACGAGATTGCGGGTTACAAGTCGGAGCTCTTTGCTAAGTTGAAGGGAAAAGCTGAGCAAGTGTTGGAAATTGGTATAGGGACTGGTCCTAATCTAAGATACTATGGTGCTGATGATGGTGTCCGAGTTTTCGGCGTGGATCCTAATAAAAAGATGGAAAGATATGCTAAGGCTGCGGCCGTGGCTGCTGGTCTGCCCCTTTCGAACTTTGAGTTTATACAAGCAGTTGGGGAGGCTATACCATTAGATGATGCTTCTGTTGATGCAGTTGTTGGAACCCTTGTATTGTGTTCTGTAAAAGATGTCAACAAGACTCTAAAAGAGATTAAGAGAGTGCTGAGACCAGGTGGACTCTACTTGTTTGTGGAGCATGTGGCTGCAAAAGATGGGACAACCCTCAGATTTATACAGAGTGTTCTTGATCCTTTGCAACAAACCCTTGCCGATGGGTGTCATCTCACCCGGGAAACAGGAACCAATATATCCAGATCCGGCTTCTCAGGCGTCGAGCTTAGCATGACCTCCTTGTCCAGTACCTCAATTATAAACCCTCAAATCTATGGAATAGCTTGCAAGCCACATGAGTCACATACGGCCAGTAGGGACTGGTCCGTTGGTCCAGACGAGCCTTGTTGGCGAACTAATACGAGCTTTTCTCCACCTCCTTCGAGATGGGATTTTCGATTCCAATCTGAAGAACTGCAATATGGTTTGCATGATGGTGTGCAACTATATGGGTCTTCTACTTCATCAAACAGTAGAGGAAGTAGGGGATGGGTGAGAGGGAATCATCTCTATAATCATCATTACTCTGCATCTGATGGTGCTGGGCTGTTTTTAAGTAGTTCGTCTGACCATTCTCAAGGTCCTCAGTGGACACCTCCAGCAATACAGGAAATCAGTATCGATGATTATGAAACTACAAAAAGGAGAGGTCCAGCTTTTGGGAAGTCATCCTTTAGACCTACCATGGAGGGAACCTCAGAAATTCAAGATACTGGGGGGTCCACGTCATCTCGTTCAGACAGTAGTGAATCTGAACCCACGGTCAAGGCATCCCTATCTTGTCATCATACTTTCGCAAGTCGCCGTTCTTTCATGTCCAAACCTATATATCCCTTGTCATTTCCTCCACAAACTCCCCCCAGAGAGGCTTCTGACTTCACAGTTGCTGGGTTTCCTGATTTTGATGCTGCCACTCCTCAGAGAGATGGCCACCGCTGGAGCAGTGCCAGCAGCAGTGTTGATTTTACTGATGTTTCTGAGTCATTTGAGGCAGAAATTTCTGGTCGACACTTTAGTAATATGTCTGAGGGGTTTAGATGTGGCTTGTGTGATAGGTTCCTTTCACAGAGATCACCTTGGAGTTCTCGTCGCATAGTCAGAAGTGGAGATATGCCAATCACTGGGGTTCTTTCTTGTTGTCATGTTTTCCATGCAGAGTGCTTGGAGCAAACAACACCGAAGATCCGCAAAAGCGACCCTCCCTGCCCCCTATGTGCCCGACTTGAGGAGGAAAATCTCTCTGAACAGCAAAGCTTTTCTAGATTGCGAGCTAGTTTTCCAAGACTTAGACCAATTAGTGATGATGGACCATCCAGGCCTTGGGGATGCGCTCAGGTGGGAGATTGTGTTGAAGGGGCTTTGCATGCTCCCCCACGCAATAGTATGCTGTTGCTCAATAAGAGTCGCATAAAGAAAAATCTTTCTTTGAAAAGCAATTCGAGCAAAGAATTTCCTGGTAAATTGAGAAAAAGCGGCGCTTACTATTCACAACATCTGAGTGGAAAATCAGTTGATCAGGGAGCAGTTGGATGCTCAAAGTCAAAGATGACAGCAGGGCCGAGTATTAAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

737

Amino Acids

80.26

Weight (kDa)

8.16

Isoelectric Point (pI)

55.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_23 PF13489 121 - 245 9.5e-12 Methyltransferase domain
Methyltransf_31 PF13847 135 - 249 2.3e-10 Methyltransferase domain
Methyltransf_25 PF13649 139 - 235 1.4e-16 Methyltransferase domain
Methyltransf_11 PF08241 140 - 239 3.7e-21 Methyltransferase domain
Methyltransf_12 PF08242 140 - 236 4.3e-11 Methyltransferase domain
Ubie_methyltran PF01209 155 - 242 2.6e-09 ubiE/COQ5 methyltransferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 899
AasI GACNNNNNNGTC 1 cut(s) 880
AccB7I CCANNNNNTGG 3 cut(s) 1298, 1760, 1959
AccBSI CCGCTC 1 cut(s) 150
AccI GTMKAC 1 cut(s) 1645
AciI CCGC 7 cut(s) 150, 165, 365, 530, 1573, 1825, 2112
AclWI GGATC 6 cut(s) 127, 488, 501, 770, 837, 1816
AcoI YGGCCR 5 cut(s) 114, 264, 531, 948, 1567
AcsI RAATTY 3 cut(s) 1341, 1635, 2087
AcuI CTGAAG 1 cut(s) 1068
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 858
AfaI GTAC 1 cut(s) 889
AfiI CCNNNNNNNGG 6 cut(s) 273, 1298, 1504, 1760, 1864, 1959
AgsI TTSAA 5 cut(s) 331, 397, 1346, 1991, 2068
AjiI CACGTC 1 cut(s) 1365
AjnI CCWGG 3 cut(s) 697, 1952, 2092
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
AluBI AGCT 9 cut(s) 382, 407, 865, 923, 1005, 1295, 1893, 1910, 2211
AluI AGCT 9 cut(s) 382, 407, 865, 923, 1005, 1295, 1893, 1910, 2211
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 688
AlwI GGATC 6 cut(s) 127, 488, 501, 770, 837, 1816
AlwNI CAGNNNCTG 1 cut(s) 1526
Ama87I CYCGRG 3 cut(s) 90, 125, 816
AoxI GGCC 8 cut(s) 114, 128, 264, 531, 948, 1567, 1955, 2195
ApeKI GCWGC 6 cut(s) 188, 527, 539, 731, 1547, 1590
ApoI RAATTY 3 cut(s) 1341, 1635, 2087
ArsI GACNNNNNNTTYG 2 cut(s) 177, 209
AspLEI GCGC 2 cut(s) 1970, 2117
AspS9I GGNCC 9 cut(s) 128, 440, 963, 971, 1220, 1289, 1359, 1946, 2195
AsuC2I CCSGG 2 cut(s) 817, 818
AsuHPI GGTGA 5 cut(s) 102, 275, 805, 1144, 1704
AsuII TTCGAA 1 cut(s) 557
AvaI CYCGRG 3 cut(s) 90, 125, 816
AvaII GGWCC 7 cut(s) 440, 963, 971, 1220, 1289, 1359, 1946
BaeGI GKGCMC 1 cut(s) 1858
BaeI ACNNNNGTAYC 2 cut(s) 445, 478
BalI TGGCCA 1 cut(s) 1569
BamHI GGATCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 384
BbrPI CACGTG 1 cut(s) 44
BbsI GAAGAC 3 cut(s) 178, 360, 1086
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 6 cut(s) 200, 514, 526, 718, 1534, 1602
BceAI ACGGC 4 cut(s) 279, 518, 963, 1428
BciT130I CCWGG 3 cut(s) 699, 1954, 2094
BclI TGATCA 1 cut(s) 2152
BcnI CCSGG 2 cut(s) 817, 818
BcoDI GTCTC 1 cut(s) 688
BfaI CTAG 2 cut(s) 1899, 1911
BfoI RGCGCY 1 cut(s) 2118
BglI GCCNNNNNGGC 1 cut(s) 988
BisI GCNGC 9 cut(s) 151, 189, 528, 531, 540, 732, 1548, 1591, 2113
BlpI GCTNAGC 2 cut(s) 408, 866
BlsI GCNGC 9 cut(s) 152, 190, 529, 532, 541, 733, 1549, 1592, 2114
Bme1390I CCNGG 5 cut(s) 699, 817, 818, 1954, 2094
Bme18I GGWCC 7 cut(s) 440, 963, 971, 1220, 1289, 1359, 1946
BmeT110I CYCGRG 3 cut(s) 90, 125, 816
BmgBI CACGTC 1 cut(s) 1365
BmgT120I GGNCC 9 cut(s) 128, 440, 963, 971, 1220, 1289, 1359, 1946, 2195
BmiI GGNNCC 6 cut(s) 495, 631, 829, 1333, 1360, 1697
BmrFI CCNGG 5 cut(s) 699, 817, 818, 1954, 2094
BmrI ACTGGG 2 cut(s) 1362, 1770
BmsI GCATC 7 cut(s) 595, 607, 1178, 1418, 1534, 1955, 2159
BmuI ACTGGG 2 cut(s) 1362, 1770
BoxI GACNNNNGTC 1 cut(s) 92
BpiI GAAGAC 3 cut(s) 178, 360, 1086
BplI GAGNNNNNCTC 4 cut(s) 994, 1026, 1319, 1351
BpmI CTGGAG 2 cut(s) 1221, 1597
Bpu10I CCTNAGC 2 cut(s) 522, 1971
Bpu1102I GCTNAGC 2 cut(s) 408, 866
Bpu14I TTCGAA 1 cut(s) 557
BpuEI CTTGAG 2 cut(s) 1200, 1883
BpuMI CCSGG 2 cut(s) 817, 818
Bsa29I ATCGAT 1 cut(s) 1260
BsaAI YACGTR 1 cut(s) 44
BsaBI GATNNNNATC 1 cut(s) 1042
BsaHI GRCGYC 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 688
BsaJI CCNNGG 6 cut(s) 534, 816, 1323, 1398, 1715, 1958
BsaXI ACNNNNNCTCC 4 cut(s) 1711, 1741, 1971, 2001
Bsc4I CCNNNNNNNGG 6 cut(s) 273, 1298, 1504, 1760, 1864, 1959
Bse118I RCCGGY 2 cut(s) 112, 335
Bse1I ACTGG 7 cut(s) 305, 442, 885, 951, 965, 1357, 1765
Bse8I GATNNNNATC 1 cut(s) 1042
BseBI CCWGG 3 cut(s) 699, 1954, 2094
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 6 cut(s) 534, 816, 1323, 1398, 1715, 1958
BseGI GGATG 7 cut(s) 26, 1133, 1307, 1409, 1949, 1970, 2174
BseJI GATNNNNATC 1 cut(s) 1042
BseLI CCNNNNNNNGG 6 cut(s) 273, 1298, 1504, 1760, 1864, 1959
BseNI ACTGG 7 cut(s) 305, 442, 885, 951, 965, 1357, 1765
BseRI GAGGAG 3 cut(s) 279, 1545, 1880
BseSI GKGCMC 1 cut(s) 1858
BseX3I CGGCCG 2 cut(s) 114, 531
BseXI GCAGC 6 cut(s) 200, 514, 526, 718, 1534, 1602
BseYI CCCAGC 2 cut(s) 1181, 1526
Bsh1285I CGRYCG 2 cut(s) 117, 534
BshFI GGCC 8 cut(s) 116, 130, 266, 533, 950, 1569, 1957, 2197
BshVI ATCGAT 1 cut(s) 1260
BsiEI CGRYCG 2 cut(s) 117, 534
BsiHKAI GWGCWC 1 cut(s) 384
BsiHKCI CYCGRG 3 cut(s) 90, 125, 816
BsiSI CCGG 7 cut(s) 30, 113, 117, 131, 336, 817, 846
BslFI GGGAC 3 cut(s) 448, 757, 971
BslI CCNNNNNNNGG 6 cut(s) 273, 1298, 1504, 1760, 1864, 1959
BsmAI GTCTC 1 cut(s) 688
BsmFI GGGAC 3 cut(s) 448, 757, 971
BsnI GGCC 8 cut(s) 116, 130, 266, 533, 950, 1569, 1957, 2197
Bso31I GGTCTC 1 cut(s) 688
BsoBI CYCGRG 3 cut(s) 90, 125, 816
Bsp119I TTCGAA 1 cut(s) 557
Bsp1286I GDGCHC 2 cut(s) 384, 1858
Bsp143I GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
Bsp1720I GCTNAGC 2 cut(s) 408, 866
Bsp19I CCATGG 1 cut(s) 1323
BspACI CCGC 7 cut(s) 150, 165, 365, 530, 1573, 1825, 2112
BspANI GGCC 8 cut(s) 116, 130, 266, 533, 950, 1569, 1957, 2197
BspDI ATCGAT 1 cut(s) 1260
BspLI GGNNCC 6 cut(s) 495, 631, 829, 1333, 1360, 1697
BspPI GGATC 6 cut(s) 127, 488, 501, 770, 837, 1816
BspT104I TTCGAA 1 cut(s) 557
BspTNI GGTCTC 1 cut(s) 688
BsrBI CCGCTC 1 cut(s) 150
BsrFI RCCGGY 2 cut(s) 112, 335
BsrI ACTGG 7 cut(s) 305, 442, 885, 951, 965, 1357, 1765
BssAI RCCGGY 2 cut(s) 112, 335
BssECI CCNNGG 6 cut(s) 534, 816, 1323, 1398, 1715, 1958
BssMI GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
BssNI GRCGYC 1 cut(s) 858
BssT1I CCWWGG 3 cut(s) 1323, 1715, 1958
Bst2UI CCWGG 3 cut(s) 699, 1954, 2094
Bst4CI ACNGT 5 cut(s) 14, 1112, 1382, 1402, 1522
Bst6I CTCTTC 2 cut(s) 26, 303
BstACI GRCGYC 1 cut(s) 858
BstBAI YACGTR 1 cut(s) 44
BstBI TTCGAA 1 cut(s) 557
BstC8I GCNNGC 6 cut(s) 341, 925, 929, 1588, 1908, 2004
BstDSI CCRYGG 3 cut(s) 534, 1323, 1398
BstF5I GGATG 7 cut(s) 26, 1133, 1307, 1409, 1949, 1970, 2174
BstH2I RGCGCY 1 cut(s) 2118
BstHHI GCGC 2 cut(s) 1970, 2117
BstKTI GATC 8 cut(s) 6, 122, 496, 778, 845, 1712, 1824, 2155
BstMAI GTCTC 1 cut(s) 688
BstMBI GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
BstMCI CGRYCG 2 cut(s) 117, 534
BstMWI GCNNNNNNNGC 9 cut(s) 113, 527, 536, 539, 855, 988, 1587, 2003, 2079
BstNI CCWGG 3 cut(s) 699, 1954, 2094
BstNSI RCATGY 3 cut(s) 343, 728, 2006
BstPAI GACNNNNGTC 1 cut(s) 92
BstSCI CCNGG 5 cut(s) 697, 815, 816, 1952, 2092
BstSLI GKGCMC 1 cut(s) 1858
BstV1I GCAGC 6 cut(s) 200, 514, 526, 718, 1534, 1602
BstV2I GAAGAC 3 cut(s) 178, 360, 1086
BstX2I RGATCY 3 cut(s) 493, 842, 1821
BstYI RGATCY 3 cut(s) 493, 842, 1821
BstZI CGGCCG 2 cut(s) 114, 531
Bsu15I ATCGAT 1 cut(s) 1260
BsuRI GGCC 8 cut(s) 116, 130, 266, 533, 950, 1569, 1957, 2197
BsuTUI ATCGAT 1 cut(s) 1260
BtgI CCRYGG 3 cut(s) 534, 1323, 1398
BtrI CACGTC 1 cut(s) 1365
BtsCI GGATG 7 cut(s) 26, 1133, 1307, 1409, 1949, 1970, 2174
BtsI GCAGTG 2 cut(s) 1588, 1600
BtsIMutI CAGTG 5 cut(s) 330, 1232, 1588, 1600, 1758
Cac8I GCNNGC 6 cut(s) 341, 925, 929, 1588, 1908, 2004
CaiI CAGNNNCTG 1 cut(s) 1526
CfoI GCGC 2 cut(s) 1970, 2117
Cfr10I RCCGGY 2 cut(s) 112, 335
Cfr13I GGNCC 9 cut(s) 128, 440, 963, 971, 1220, 1289, 1359, 1946, 2195
Cfr9I CCCGGG 1 cut(s) 816
ClaI ATCGAT 1 cut(s) 1260
CseI GACGC 4 cut(s) 113, 183, 309, 847
CsiI ACCWGGT 1 cut(s) 697
Csp6I GTAC 1 cut(s) 888
CviQI GTAC 1 cut(s) 888
DpnI GATC 8 cut(s) 5, 121, 495, 777, 844, 1711, 1823, 2154
DpnII GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
DrdI GACNNNNNNGTC 1 cut(s) 880
DseDI GACNNNNNNGTC 1 cut(s) 880
EaeI YGGCCR 5 cut(s) 114, 264, 531, 948, 1567
EagI CGGCCG 2 cut(s) 114, 531
Eam1104I CTCTTC 2 cut(s) 26, 303
EarI CTCTTC 2 cut(s) 26, 303
Ecl136II GAGCTC 1 cut(s) 382
EclXI CGGCCG 2 cut(s) 114, 531
Eco130I CCWWGG 3 cut(s) 1323, 1715, 1958
Eco147I AGGCCT 1 cut(s) 1957
Eco24I GRGCYC 1 cut(s) 384
Eco31I GGTCTC 1 cut(s) 688
Eco47I GGWCC 7 cut(s) 440, 963, 971, 1220, 1289, 1359, 1946
Eco52I CGGCCG 2 cut(s) 114, 531
Eco53kI GAGCTC 1 cut(s) 382
Eco57I CTGAAG 1 cut(s) 1068
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 3 cut(s) 90, 125, 816
EcoICRI GAGCTC 1 cut(s) 382
EcoO109I RGGNCCY 1 cut(s) 1220
EcoRII CCWGG 3 cut(s) 697, 1952, 2092
EcoT14I CCWWGG 3 cut(s) 1323, 1715, 1958
EcoT38I GRGCYC 1 cut(s) 384
ErhI CCWWGG 3 cut(s) 1323, 1715, 1958
FalI AAGNNNNNCTT 4 cut(s) 1295, 1327, 2101, 2133
FaqI GGGAC 3 cut(s) 448, 757, 971
FauI CCCGC 1 cut(s) 358
FbaI TGATCA 1 cut(s) 2152
FblI GTMKAC 1 cut(s) 1645
Fnu4HI GCNGC 9 cut(s) 151, 189, 528, 531, 540, 732, 1548, 1591, 2113
FokI GGATG 7 cut(s) 13, 1140, 1294, 1396, 1936, 1977, 2181
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 9 cut(s) 151, 189, 528, 531, 540, 732, 1548, 1591, 2113
FspBI CTAG 2 cut(s) 1899, 1911
GlaI GCGC 2 cut(s) 1969, 2116
GluI GCNGC 9 cut(s) 151, 189, 528, 531, 540, 732, 1548, 1591, 2113
GsaI CCCAGC 2 cut(s) 1185, 1530
GsuI CTGGAG 2 cut(s) 1221, 1597
HaeII RGCGCY 1 cut(s) 2118
HaeIII GGCC 8 cut(s) 116, 130, 266, 533, 950, 1569, 1957, 2197
HapII CCGG 7 cut(s) 30, 113, 117, 131, 336, 817, 846
HgaI GACGC 4 cut(s) 113, 183, 309, 847
HhaI GCGC 2 cut(s) 1970, 2117
Hin1I GRCGYC 1 cut(s) 858
Hin6I GCGC 2 cut(s) 1968, 2115
HinP1I GCGC 2 cut(s) 1968, 2115
HincII GTYRAC 3 cut(s) 97, 661, 1646
HindII GTYRAC 3 cut(s) 97, 661, 1646
HindIII AAGCTT 1 cut(s) 1891
HpaII CCGG 7 cut(s) 30, 113, 117, 131, 336, 817, 846
HphI GGTGA 5 cut(s) 102, 275, 805, 1144, 1704
Hpy166II GTNNAC 7 cut(s) 97, 283, 661, 704, 1230, 1362, 1646
Hpy188III TCNNGA 7 cut(s) 773, 840, 974, 1023, 1346, 1535, 1899
Hpy8I GTNNAC 7 cut(s) 97, 283, 661, 704, 1230, 1362, 1646
Hpy99I CGWCG 3 cut(s) 257, 863, 1731
HpyAV CCTTC 4 cut(s) 241, 391, 1029, 1985
HpyCH4III ACNGT 5 cut(s) 14, 1112, 1382, 1402, 1522
HpyCH4IV ACGT 3 cut(s) 43, 269, 1364
HpyF10VI GCNNNNNNNGC 9 cut(s) 113, 527, 536, 539, 855, 988, 1587, 2003, 2079
HpySE526I ACGT 3 cut(s) 43, 269, 1364
Hsp92I GRCGYC 1 cut(s) 858
HspAI GCGC 2 cut(s) 1968, 2115
Ksp22I TGATCA 1 cut(s) 2152
Kzo9I GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
LmnI GCTCC 6 cut(s) 379, 721, 1578, 1804, 2011, 2159
Lsp1109I GCAGC 6 cut(s) 200, 514, 526, 718, 1534, 1602
LweI GCATC 7 cut(s) 595, 607, 1178, 1418, 1534, 1955, 2159
MabI ACCWGGT 1 cut(s) 697
MaeI CTAG 2 cut(s) 1899, 1911
MaeII ACGT 3 cut(s) 43, 269, 1364
MaeIII GTNAC 4 cut(s) 34, 193, 368, 939
MalI GATC 8 cut(s) 5, 121, 495, 777, 844, 1711, 1823, 2154
MbiI CCGCTC 1 cut(s) 150
MboI GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
MflI RGATCY 3 cut(s) 493, 842, 1821
MhlI GDGCHC 2 cut(s) 384, 1858
MlsI TGGCCA 1 cut(s) 1569
MluCI AATT 9 cut(s) 213, 423, 894, 1341, 1635, 1932, 2074, 2087, 2099
MluNI TGGCCA 1 cut(s) 1569
MlyI GAGTC 7 cut(s) 102, 128, 661, 699, 947, 1628, 2050
MmeI TCCRAC 5 cut(s) 110, 357, 399, 607, 2146
Mox20I TGGCCA 1 cut(s) 1569
MscI TGGCCA 1 cut(s) 1569
MseI TTAA 3 cut(s) 681, 1193, 2205
MslI CAYNNNNRTG 3 cut(s) 338, 1074, 2136
Msp20I TGGCCA 1 cut(s) 1569
MspA1I CMGCKG 1 cut(s) 1575
MspI CCGG 7 cut(s) 30, 113, 117, 131, 336, 817, 846
MspR9I CCNGG 5 cut(s) 699, 817, 818, 1954, 2094
MvaI CCWGG 3 cut(s) 699, 1954, 2094
MwoI GCNNNNNNNGC 9 cut(s) 113, 527, 536, 539, 855, 988, 1587, 2003, 2079
NciI CCSGG 2 cut(s) 817, 818
NcoI CCATGG 1 cut(s) 1323
NdeII GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
NlaIV GGNNCC 6 cut(s) 495, 631, 829, 1333, 1360, 1697
NmuCI GTSAC 2 cut(s) 193, 939
NspI RCATGY 3 cut(s) 343, 728, 2006
NspV TTCGAA 1 cut(s) 557
PaeI GCATGC 2 cut(s) 343, 2006
PceI AGGCCT 1 cut(s) 1957
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PfeI GAWTC 3 cut(s) 1038, 1141, 1388
PflMI CCANNNNNTGG 3 cut(s) 1298, 1760, 1959
PkrI GCNGC 9 cut(s) 152, 190, 529, 532, 541, 733, 1549, 1592, 2114
PleI GAGTC 7 cut(s) 101, 128, 661, 699, 946, 1627, 2049
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 7 cut(s) 101, 128, 661, 699, 946, 1627, 2049
Ppu21I YACGTR 1 cut(s) 44
PpuMI RGGWCCY 1 cut(s) 1220
PshAI GACNNNNGTC 1 cut(s) 92
PsiI TTATAA 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 384
Psp5II RGGWCCY 1 cut(s) 1220
Psp6I CCWGG 3 cut(s) 697, 1952, 2092
PspCI CACGTG 1 cut(s) 44
PspFI CCCAGC 2 cut(s) 1181, 1526
PspGI CCWGG 3 cut(s) 697, 1952, 2092
PspN4I GGNNCC 6 cut(s) 495, 631, 829, 1333, 1360, 1697
PspPI GGNCC 9 cut(s) 128, 440, 963, 971, 1220, 1289, 1359, 1946, 2195
PspPPI RGGWCCY 1 cut(s) 1220
PstNI CAGNNNCTG 1 cut(s) 1526
PsuI RGATCY 3 cut(s) 493, 842, 1821
RsaI GTAC 1 cut(s) 889
RsaNI GTAC 1 cut(s) 888
RseI CAYNNNNRTG 3 cut(s) 338, 1074, 2136
SacI GAGCTC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 1644
SaqAI TTAA 3 cut(s) 681, 1193, 2205
SatI GCNGC 9 cut(s) 151, 189, 528, 531, 540, 732, 1548, 1591, 2113
Sau3AI GATC 8 cut(s) 3, 119, 493, 775, 842, 1709, 1821, 2152
Sau96I GGNCC 9 cut(s) 128, 440, 963, 971, 1220, 1289, 1359, 1946, 2195
SchI GAGTC 7 cut(s) 102, 128, 661, 699, 947, 1628, 2050
ScrFI CCNGG 5 cut(s) 699, 817, 818, 1954, 2094
SduI GDGCHC 2 cut(s) 384, 1858
SexAI ACCWGGT 1 cut(s) 697
SfaNI GCATC 7 cut(s) 595, 607, 1178, 1418, 1534, 1955, 2159
SfuI TTCGAA 1 cut(s) 557
SinI GGWCC 7 cut(s) 440, 963, 971, 1220, 1289, 1359, 1946
SmaI CCCGGG 1 cut(s) 818
SmiMI CAYNNNNRTG 3 cut(s) 338, 1074, 2136
SmlI CTYRAG 2 cut(s) 1215, 1862
SmoI CTYRAG 2 cut(s) 1215, 1862
SphI GCATGC 2 cut(s) 343, 2006
Sse9I AATT 9 cut(s) 213, 423, 894, 1341, 1635, 1932, 2074, 2087, 2099
SseBI AGGCCT 1 cut(s) 1957
SsiI CCGC 7 cut(s) 150, 165, 365, 530, 1573, 1825, 2112
SspMI CTAG 2 cut(s) 1899, 1911
SstI GAGCTC 1 cut(s) 384
StuI AGGCCT 1 cut(s) 1957
StyD4I CCNGG 5 cut(s) 697, 815, 816, 1952, 2092
StyI CCWWGG 3 cut(s) 1323, 1715, 1958
TaaI ACNGT 5 cut(s) 14, 1112, 1382, 1402, 1522
TaiI ACGT 3 cut(s) 46, 272, 1367
TasI AATT 9 cut(s) 213, 423, 894, 1341, 1635, 1932, 2074, 2087, 2099
TauI GCSGC 3 cut(s) 153, 533, 2115
TfiI GAWTC 3 cut(s) 1038, 1141, 1388
Tru1I TTAA 3 cut(s) 681, 1193, 2205
Tru9I TTAA 3 cut(s) 681, 1193, 2205
TscAI CASTG 5 cut(s) 330, 1232, 1588, 1600, 1765
TseFI GTSAC 2 cut(s) 193, 939
TseI GCWGC 6 cut(s) 188, 527, 539, 731, 1547, 1590
Tsp45I GTSAC 2 cut(s) 193, 939
TspDTI ATGAA 6 cut(s) 169, 189, 321, 1092, 1284, 1441
TspGWI ACGGA 1 cut(s) 955
TspMI CCCGGG 1 cut(s) 816
TspRI CASTG 5 cut(s) 330, 1232, 1588, 1600, 1765
Van91I CCANNNNNTGG 3 cut(s) 1298, 1760, 1959
VpaK11BI GGWCC 7 cut(s) 440, 963, 971, 1220, 1289, 1359, 1946
XapI RAATTY 3 cut(s) 1341, 1635, 2087
XbaI TCTAGA 1 cut(s) 1898
XceI RCATGY 3 cut(s) 343, 728, 2006
XcmI CCANNNNNNNNNTGG 1 cut(s) 1956
XmaI CCCGGG 1 cut(s) 816
XmiI GTMKAC 1 cut(s) 1645
XspI CTAG 2 cut(s) 1899, 1911
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.