Rh5DG021300

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0010019
Physical Location & Seq
Reverse (-)
1534870 .. 1535691
822 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG021300.1

Sequence Viewer

Length: 822 bp
ATGGCTTCTCCCTTCACCTACAAATTCATTTTACTCATTTCCTTTCAATTTCTCAGCCTCACAGCAGCTCAAGCCAGCCCATGCAGACCTTTTTGTGGTAACATTCCGATTAACTACCCTTTCGGCATTGATGAAGGCTGTGGCAGCCCTTATTACAGTCACCTTCTTGTCTGCTCTGAGCCGGGTAAACTTGAGCTCCGAACACCTTCTGGCAGATACCCTGTCAAGAGCATAAGCTACTCCGATCCTCACATTCTTGTCACCGATCCTTTCATGTGGAATTGCCTAGACGGTGACAGTTTTCGACCAACTAGGCCTTTCAGCCTTGACACTAGTACACATTTGTCACTCTCTTCTCAAAATGACTACCTTTTCTTCAATTGTAGTGAAGAAAATGTGATCGTTCAGCCAAAGCCTATGTTCTGTGAAAGGTACCCCGAAAAGTGTGACTCATCATGTGACAGTTCTAGTTATCTTTGCAGGCACTTACCTGAGTGTGCCTCAGCATTGAAAAGAAGTACTTGCTGTTCTTACTATCCCAAAGCCACTGAATCCCTAAGGTTGATGTTGAAGTACTGTGCTAGTTATACTAGTGTGCATTGGAGAAACACCGGTGAAACACAGCCTGATGATCAAGTCCCCGAATATGGGATTCGGGTGGATTTTGATATTCCAGTTACCACACGTTGCCTTCACTGCCAGGACACATCCAAAGGTGGTGGAACTTGTGGATTCGACACCAAAACACAGAGTTTCTTGTGCCTATGTGAGAGAGGAAATGTGACTACTTATTGTAATGGTAAGTATGAGGCATTTGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

273

Amino Acids

30.74

Weight (kDa)

5.87

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 89 4.2e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AclWI GGATC 2 cut(s) 239, 260
AcsI RAATTY 1 cut(s) 23
AfaI GTAC 4 cut(s) 337, 434, 520, 575
AfiI CCNNNNNNNGG 3 cut(s) 95, 647, 648
AflIII ACRYGT 1 cut(s) 683
AgeI ACCGGT 1 cut(s) 611
AgsI TTSAA 4 cut(s) 47, 379, 511, 571
AhlI ACTAGT 2 cut(s) 332, 590
AjnI CCWGG 1 cut(s) 699
AluBI AGCT 3 cut(s) 68, 196, 237
AluI AGCT 3 cut(s) 68, 196, 237
Alw21I GWGCWC 1 cut(s) 198
AlwI GGATC 2 cut(s) 239, 260
AoxI GGCC 1 cut(s) 314
ApeKI GCWGC 2 cut(s) 65, 144
ApoI RAATTY 1 cut(s) 23
AsiGI ACCGGT 1 cut(s) 611
Asp700I GAANNNNTTC 1 cut(s) 205
Asp718I GGTACC 1 cut(s) 432
AsuC2I CCSGG 1 cut(s) 183
AsuHPI GGTGA 5 cut(s) 7, 152, 253, 305, 626
AxyI CCTNAGG 1 cut(s) 557
BanI GGYRCC 1 cut(s) 432
BanII GRGCYC 1 cut(s) 198
Bbv12I GWGCWC 1 cut(s) 198
BbvCI CCTCAGC 1 cut(s) 502
BbvI GCAGC 2 cut(s) 77, 156
BciT130I CCWGG 1 cut(s) 701
BclI TGATCA 1 cut(s) 631
BcnI CCSGG 1 cut(s) 183
BcuI ACTAGT 2 cut(s) 332, 590
BfaI CTAG 6 cut(s) 287, 312, 333, 468, 582, 591
BisI GCNGC 2 cut(s) 66, 145
BlsI GCNGC 2 cut(s) 67, 146
BmcAI AGTACT 2 cut(s) 520, 575
Bme1390I CCNGG 2 cut(s) 183, 701
BmiI GGNNCC 1 cut(s) 434
BmrFI CCNGG 2 cut(s) 183, 701
BplI GAGNNNNNCTC 2 cut(s) 485, 517
Bpu10I CCTNAGC 1 cut(s) 502
BpuEI CTTGAG 2 cut(s) 54, 212
BpuMI CCSGG 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 611
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 647, 648
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 1 cut(s) 674
Bse21I CCTNAGG 1 cut(s) 557
BseBI CCWGG 1 cut(s) 701
BseGI GGATG 1 cut(s) 707
BseLI CCNNNNNNNGG 3 cut(s) 95, 647, 648
BseMII CTCAG 4 cut(s) 67, 168, 483, 516
BseNI ACTGG 1 cut(s) 674
BseXI GCAGC 2 cut(s) 77, 156
BshFI GGCC 1 cut(s) 316
BshNI GGYRCC 1 cut(s) 432
BshTI ACCGGT 1 cut(s) 611
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 2 cut(s) 182, 612
BslFI GGGAC 1 cut(s) 623
BslI CCNNNNNNNGG 3 cut(s) 95, 647, 648
BsmFI GGGAC 1 cut(s) 623
BsnI GGCC 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 4 cut(s) 244, 265, 399, 631
BspANI GGCC 1 cut(s) 316
BspCNI CTCAG 4 cut(s) 66, 169, 484, 515
BspLI GGNNCC 1 cut(s) 434
BspPI GGATC 2 cut(s) 239, 260
BspT107I GGYRCC 1 cut(s) 432
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 1 cut(s) 674
BssAI RCCGGY 1 cut(s) 611
BssMI GATC 4 cut(s) 244, 265, 399, 631
Bst2UI CCWGG 1 cut(s) 701
Bst4CI ACNGT 5 cut(s) 158, 293, 299, 464, 578
Bst6I CTCTTC 1 cut(s) 358
BstC8I GCNNGC 2 cut(s) 76, 482
BstDEI CTNAG 5 cut(s) 53, 177, 492, 502, 557
BstF5I GGATG 1 cut(s) 707
BstKTI GATC 4 cut(s) 247, 268, 402, 634
BstMBI GATC 4 cut(s) 244, 265, 399, 631
BstMWI GCNNNNNNNGC 3 cut(s) 71, 144, 696
BstNI CCWGG 1 cut(s) 701
BstSCI CCNGG 2 cut(s) 181, 699
BstV1I GCAGC 2 cut(s) 77, 156
Bsu36I CCTNAGG 1 cut(s) 557
BsuRI GGCC 1 cut(s) 316
BtsCI GGATG 1 cut(s) 707
BtsI GCAGTG 1 cut(s) 694
BtsIMutI CAGTG 2 cut(s) 546, 694
Cac8I GCNNGC 2 cut(s) 76, 482
Cfr10I RCCGGY 1 cut(s) 611
Csp6I GTAC 4 cut(s) 336, 433, 519, 574
CspAI ACCGGT 1 cut(s) 611
CspCI CAANNNNNGTGG 2 cut(s) 700, 735
CviAII CATG 3 cut(s) 81, 274, 456
CviQI GTAC 4 cut(s) 336, 433, 519, 574
DdeI CTNAG 5 cut(s) 53, 177, 492, 502, 557
DpnI GATC 4 cut(s) 246, 267, 401, 633
DpnII GATC 4 cut(s) 244, 265, 399, 631
Eam1104I CTCTTC 1 cut(s) 358
EarI CTCTTC 1 cut(s) 358
Ecl136II GAGCTC 1 cut(s) 196
Eco147I AGGCCT 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 198
Eco53kI GAGCTC 1 cut(s) 196
Eco81I CCTNAGG 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 3 cut(s) 84, 277, 459
FalI AAGNNNNNCTT 2 cut(s) 505, 537
FaqI GGGAC 1 cut(s) 623
FatI CATG 3 cut(s) 80, 273, 455
FbaI TGATCA 1 cut(s) 631
Fnu4HI GCNGC 2 cut(s) 66, 145
FokI GGATG 1 cut(s) 694
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 2 cut(s) 66, 145
FspBI CTAG 6 cut(s) 287, 312, 333, 468, 582, 591
GluI GCNGC 2 cut(s) 66, 145
HaeIII GGCC 1 cut(s) 316
HapII CCGG 2 cut(s) 182, 612
Hin1II CATG 3 cut(s) 84, 277, 459
HinfI GANTC 4 cut(s) 449, 551, 652, 732
HpaII CCGG 2 cut(s) 182, 612
HphI GGTGA 5 cut(s) 7, 152, 253, 305, 626
Hpy166II GTNNAC 2 cut(s) 188, 338
Hpy188I TCNGA 4 cut(s) 108, 178, 200, 244
Hpy188III TCNNGA 1 cut(s) 226
Hpy8I GTNNAC 2 cut(s) 188, 338
HpyAV CCTTC 5 cut(s) 22, 128, 173, 216, 701
HpyCH4III ACNGT 5 cut(s) 158, 293, 299, 464, 578
HpyCH4IV ACGT 1 cut(s) 685
HpyCH4V TGCA 3 cut(s) 84, 480, 598
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 144, 696
HpyF3I CTNAG 5 cut(s) 53, 177, 492, 502, 557
HpySE526I ACGT 1 cut(s) 685
Hsp92II CATG 3 cut(s) 84, 277, 459
KpnI GGTACC 1 cut(s) 436
Ksp22I TGATCA 1 cut(s) 631
Kzo9I GATC 4 cut(s) 244, 265, 399, 631
LmnI GCTCC 1 cut(s) 201
Lsp1109I GCAGC 2 cut(s) 77, 156
MaeI CTAG 6 cut(s) 287, 312, 333, 468, 582, 591
MaeII ACGT 1 cut(s) 685
MaeIII GTNAC 9 cut(s) 98, 158, 259, 293, 345, 446, 458, 676, 781
MalI GATC 4 cut(s) 246, 267, 401, 633
MboI GATC 4 cut(s) 244, 265, 399, 631
MboII GAAGA 3 cut(s) 345, 367, 401
MfeI CAATTG 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 198
MluCI AATT 4 cut(s) 23, 47, 280, 379
MlyI GAGTC 1 cut(s) 443
MnlI CCTC 5 cut(s) 68, 258, 511, 767, 802
MroXI GAANNNNTTC 1 cut(s) 205
MseI TTAA 1 cut(s) 111
MspI CCGG 2 cut(s) 182, 612
MspR9I CCNGG 2 cut(s) 183, 701
MunI CAATTG 1 cut(s) 379
MvaI CCWGG 1 cut(s) 701
MwoI GCNNNNNNNGC 3 cut(s) 71, 144, 696
NciI CCSGG 1 cut(s) 183
NdeII GATC 4 cut(s) 244, 265, 399, 631
NlaIII CATG 3 cut(s) 84, 277, 459
NlaIV GGNNCC 1 cut(s) 434
NmuCI GTSAC 7 cut(s) 158, 259, 293, 345, 446, 458, 781
PceI AGGCCT 1 cut(s) 316
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 3 cut(s) 551, 652, 732
PinAI ACCGGT 1 cut(s) 611
PkrI GCNGC 2 cut(s) 67, 146
PleI GAGTC 1 cut(s) 443
PpsI GAGTC 1 cut(s) 443
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 699
PspGI CCWGG 1 cut(s) 699
PspN4I GGNNCC 1 cut(s) 434
PsrI GAACNNNNNNTAC 2 cut(s) 511, 543
RsaI GTAC 4 cut(s) 337, 434, 520, 575
RsaNI GTAC 4 cut(s) 336, 433, 519, 574
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 111
SatI GCNGC 2 cut(s) 66, 145
Sau3AI GATC 4 cut(s) 244, 265, 399, 631
ScaI AGTACT 2 cut(s) 520, 575
SchI GAGTC 1 cut(s) 443
ScrFI CCNGG 2 cut(s) 183, 701
SduI GDGCHC 1 cut(s) 198
SgrAI CRCCGGYG 1 cut(s) 611
SmlI CTYRAG 2 cut(s) 69, 191
SmoI CTYRAG 2 cut(s) 69, 191
SpeI ACTAGT 2 cut(s) 332, 590
Sse9I AATT 4 cut(s) 23, 47, 280, 379
SseBI AGGCCT 1 cut(s) 316
SspMI CTAG 6 cut(s) 287, 312, 333, 468, 582, 591
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 1 cut(s) 316
StyD4I CCNGG 2 cut(s) 181, 699
TaaI ACNGT 5 cut(s) 158, 293, 299, 464, 578
TaiI ACGT 1 cut(s) 688
TaqI TCGA 2 cut(s) 304, 735
TasI AATT 4 cut(s) 23, 47, 280, 379
TatI WGTACW 3 cut(s) 335, 518, 573
TfiI GAWTC 3 cut(s) 551, 652, 732
Tru1I TTAA 1 cut(s) 111
Tru9I TTAA 1 cut(s) 111
TscAI CASTG 2 cut(s) 553, 701
TseFI GTSAC 7 cut(s) 158, 259, 293, 345, 446, 458, 781
TseI GCWGC 2 cut(s) 65, 144
Tsp45I GTSAC 7 cut(s) 158, 259, 293, 345, 446, 458, 781
TspDTI ATGAA 3 cut(s) 16, 147, 262
TspRI CASTG 2 cut(s) 553, 701
XapI RAATTY 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 6 cut(s) 287, 312, 333, 468, 582, 591
ZrmI AGTACT 2 cut(s) 520, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.