Rh5BG022900

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010019
Physical Location & Seq
Reverse (-)
1660546 .. 1661704
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG022900.1

Sequence Viewer

Length: 978 bp
ATGTCTCTAATGGCTTCTCCCTTCACCTACAAATTCATTTTACTCATTTCCTTTCAATTTCTCAGCCTCACAGCAGCTCAAGCCAGCCCATGCAGACCTTTTTGTGGTAACATTCCGATTAACTACCCTTTCGGCATTGATGAAGGCTGTGGCAGCCCTTATTACAGTCACCTTCTTCTCTGCTCTGAGCCGGGTAAACTTGAGCTCCGAACACCATCTGGCAGATACCCTGTCAAGAGCATAAGCTACTCCGATCCTCACATTCTTGTCACCGATCCTTTCATGTGGAATTGCCTAGACGGTGACAGTTTTCGACCAACTAGGCCTTTCAGCCTTGACACTAGTACACATTTGTCACTCTCTTCTCAAAATGACTACCTTTTCTTCAATTGTAGTGAAGAAAATGTGATCGTTCAGCCAAAGCCTATGTTCTGTGAAAGGTACCCCGAAAAGTGTGACTCATCATGTGACAGTTCTAGTTATCTTTGCAGGCACTTACCTGAGTGTGCCTCAGCATTGAAAAGAAGTACTTGCTGTTCTTACTATCCCAAAGCCACTGAATCCCTAAGGTTGATGTTGAAGTACTGTGCTAGTTATACTAGTGTGCATTGGAGAAACACCGGTGAAACACAGCCTGATGATCAAGTCCCCGAATATGGGATTCGGGTGGATTTTGATATTCCAGTTACCACACGTTGCCTTCACTGCCAGGACACATCCAAAGGTGGTGGAACTTGTGGATTCGACACCAAAACACAGAGTTTCTTGTGCCTATGTGAGAGAGGAAATGTGACTACTTATTGTAATGATGATAGCATTTCTAAGCATACGAGGAACGGAGTAATTGCAGGGACAGTTGCTGGTGTTTCAGTAGCAGGAGCCTTTGGAATTGGGGCAGGAATTTGGTACTTCAGGAAAATTAGAGCTAAAGCACCAGTCACATGTGGGGTTCAAAGCAATGAGAATAGGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

325

Amino Acids

36.14

Weight (kDa)

6.97

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 92 8.6e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AclWI GGATC 2 cut(s) 248, 269
AcsI RAATTY 2 cut(s) 32, 900
AcuI CTGAAG 1 cut(s) 895
AfaI GTAC 5 cut(s) 346, 443, 529, 584, 908
AfiI CCNNNNNNNGG 3 cut(s) 104, 656, 657
AflIII ACRYGT 2 cut(s) 692, 941
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 5 cut(s) 56, 388, 520, 580, 953
AhlI ACTAGT 2 cut(s) 341, 599
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 4 cut(s) 77, 205, 246, 926
AluI AGCT 4 cut(s) 77, 205, 246, 926
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 248, 269
AlwNI CAGNNNCTG 1 cut(s) 860
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 2 cut(s) 74, 153
ApoI RAATTY 2 cut(s) 32, 900
AsiGI ACCGGT 1 cut(s) 620
Asp718I GGTACC 1 cut(s) 441
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 5 cut(s) 16, 161, 262, 314, 635
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 441
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvCI CCTCAGC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 86, 165
BccI CCATC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 710
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 9
BcuI ACTAGT 2 cut(s) 341, 599
BfaI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
BisI GCNGC 2 cut(s) 75, 154
BlsI GCNGC 2 cut(s) 76, 155
BmcAI AGTACT 2 cut(s) 529, 584
Bme1390I CCNGG 2 cut(s) 192, 710
BmiI GGNNCC 2 cut(s) 443, 880
BmrFI CCNGG 2 cut(s) 192, 710
BplI GAGNNNNNCTC 2 cut(s) 494, 526
Bpu10I CCTNAGC 1 cut(s) 511
BpuEI CTTGAG 2 cut(s) 63, 221
BpuMI CCSGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 656, 657
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 2 cut(s) 683, 935
Bse21I CCTNAGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 964
BseBI CCWGG 1 cut(s) 710
BseGI GGATG 1 cut(s) 716
BseLI CCNNNNNNNGG 3 cut(s) 104, 656, 657
BseMI GCAATG 1 cut(s) 964
BseMII CTCAG 4 cut(s) 76, 177, 492, 525
BseNI ACTGG 2 cut(s) 683, 935
BseXI GCAGC 2 cut(s) 86, 165
BshFI GGCC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 441
BshTI ACCGGT 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 2 cut(s) 191, 621
BslFI GGGAC 2 cut(s) 632, 865
BslI CCNNNNNNNGG 3 cut(s) 104, 656, 657
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 632, 865
BsnI GGCC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 4 cut(s) 253, 274, 408, 640
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 4 cut(s) 75, 178, 493, 524
BspLI GGNNCC 2 cut(s) 443, 880
BspPI GGATC 2 cut(s) 248, 269
BspT107I GGYRCC 1 cut(s) 441
BsrDI GCAATG 1 cut(s) 964
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 2 cut(s) 683, 935
BssAI RCCGGY 1 cut(s) 620
BssMI GATC 4 cut(s) 253, 274, 408, 640
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 6 cut(s) 167, 302, 308, 473, 587, 856
Bst6I CTCTTC 1 cut(s) 367
BstC8I GCNNGC 2 cut(s) 85, 491
BstDEI CTNAG 6 cut(s) 62, 186, 501, 511, 566, 822
BstF5I GGATG 1 cut(s) 716
BstKTI GATC 4 cut(s) 256, 277, 411, 643
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 253, 274, 408, 640
BstMWI GCNNNNNNNGC 3 cut(s) 80, 153, 705
BstNI CCWGG 1 cut(s) 710
BstNSI RCATGY 1 cut(s) 945
BstSCI CCNGG 2 cut(s) 190, 708
BstV1I GCAGC 2 cut(s) 86, 165
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 325
BtsCI GGATG 1 cut(s) 716
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 555, 703
Cac8I GCNNGC 2 cut(s) 85, 491
CaiI CAGNNNCTG 1 cut(s) 860
Cfr10I RCCGGY 1 cut(s) 620
Csp6I GTAC 5 cut(s) 345, 442, 528, 583, 907
CspAI ACCGGT 1 cut(s) 620
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviAII CATG 4 cut(s) 90, 283, 465, 942
CviQI GTAC 5 cut(s) 345, 442, 528, 583, 907
DdeI CTNAG 6 cut(s) 62, 186, 501, 511, 566, 822
DpnI GATC 4 cut(s) 255, 276, 410, 642
DpnII GATC 4 cut(s) 253, 274, 408, 640
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco57I CTGAAG 1 cut(s) 895
Eco81I CCTNAGG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 708
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 4 cut(s) 93, 286, 468, 945
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FaqI GGGAC 2 cut(s) 632, 865
FatI CATG 4 cut(s) 89, 282, 464, 941
FbaI TGATCA 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 75, 154
FokI GGATG 1 cut(s) 703
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 75, 154
FspBI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
GluI GCNGC 2 cut(s) 75, 154
HaeIII GGCC 1 cut(s) 325
HapII CCGG 2 cut(s) 191, 621
Hin1II CATG 4 cut(s) 93, 286, 468, 945
HinfI GANTC 4 cut(s) 458, 560, 661, 741
HpaII CCGG 2 cut(s) 191, 621
HphI GGTGA 5 cut(s) 16, 161, 262, 314, 635
Hpy166II GTNNAC 2 cut(s) 197, 347
Hpy188I TCNGA 4 cut(s) 117, 187, 209, 253
Hpy188III TCNNGA 2 cut(s) 235, 913
Hpy8I GTNNAC 2 cut(s) 197, 347
HpyAV CCTTC 4 cut(s) 31, 137, 182, 710
HpyCH4III ACNGT 6 cut(s) 167, 302, 308, 473, 587, 856
HpyCH4IV ACGT 1 cut(s) 694
HpyCH4V TGCA 4 cut(s) 93, 489, 607, 848
HpyF10VI GCNNNNNNNGC 3 cut(s) 80, 153, 705
HpyF3I CTNAG 6 cut(s) 62, 186, 501, 511, 566, 822
HpySE526I ACGT 1 cut(s) 694
Hsp92II CATG 4 cut(s) 93, 286, 468, 945
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 4 cut(s) 253, 274, 408, 640
LmnI GCTCC 2 cut(s) 210, 878
Lsp1109I GCAGC 2 cut(s) 86, 165
MaeI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
MaeII ACGT 1 cut(s) 694
MalI GATC 4 cut(s) 255, 276, 410, 642
MboI GATC 4 cut(s) 253, 274, 408, 640
MboII GAAGA 4 cut(s) 167, 354, 376, 410
MfeI CAATTG 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 8 cut(s) 32, 56, 289, 388, 843, 888, 900, 918
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 77, 267, 520, 776, 825
MseI TTAA 1 cut(s) 120
MspI CCGG 2 cut(s) 191, 621
MspR9I CCNGG 2 cut(s) 192, 710
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 710
MwoI GCNNNNNNNGC 3 cut(s) 80, 153, 705
NciI CCSGG 1 cut(s) 192
NdeII GATC 4 cut(s) 253, 274, 408, 640
NlaIII CATG 4 cut(s) 93, 286, 468, 945
NlaIV GGNNCC 2 cut(s) 443, 880
NmuCI GTSAC 8 cut(s) 167, 268, 302, 354, 455, 467, 790, 937
NspI RCATGY 1 cut(s) 945
PceI AGGCCT 1 cut(s) 325
PciI ACATGT 1 cut(s) 941
PfeI GAWTC 3 cut(s) 560, 661, 741
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 2 cut(s) 76, 155
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PscI ACATGT 1 cut(s) 941
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 708
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 2 cut(s) 443, 880
PsrI GAACNNNNNNTAC 2 cut(s) 520, 552
PstNI CAGNNNCTG 1 cut(s) 860
RsaI GTAC 5 cut(s) 346, 443, 529, 584, 908
RsaNI GTAC 5 cut(s) 345, 442, 528, 583, 907
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 2 cut(s) 75, 154
Sau3AI GATC 4 cut(s) 253, 274, 408, 640
ScaI AGTACT 2 cut(s) 529, 584
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 192, 710
SduI GDGCHC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 620
SmlI CTYRAG 2 cut(s) 78, 200
SmoI CTYRAG 2 cut(s) 78, 200
SpeI ACTAGT 2 cut(s) 341, 599
Sse9I AATT 8 cut(s) 32, 56, 289, 388, 843, 888, 900, 918
SseBI AGGCCT 1 cut(s) 325
SspMI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 190, 708
TaaI ACNGT 6 cut(s) 167, 302, 308, 473, 587, 856
TaiI ACGT 1 cut(s) 697
TaqI TCGA 2 cut(s) 313, 744
TasI AATT 8 cut(s) 32, 56, 289, 388, 843, 888, 900, 918
TatI WGTACW 3 cut(s) 344, 527, 582
TfiI GAWTC 3 cut(s) 560, 661, 741
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
TscAI CASTG 2 cut(s) 562, 710
TseFI GTSAC 8 cut(s) 167, 268, 302, 354, 455, 467, 790, 937
TseI GCWGC 2 cut(s) 74, 153
Tsp45I GTSAC 8 cut(s) 167, 268, 302, 354, 455, 467, 790, 937
TspDTI ATGAA 3 cut(s) 25, 156, 271
TspGWI ACGGA 1 cut(s) 852
TspRI CASTG 2 cut(s) 562, 710
XapI RAATTY 2 cut(s) 32, 900
XceI RCATGY 1 cut(s) 945
XspI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
ZrmI AGTACT 2 cut(s) 529, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.