Rh5CG022200

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010019
Physical Location & Seq
Reverse (-)
1425878 .. 1427824
1947 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG022200.1

Sequence Viewer

Length: 849 bp
ATGTCTCTAATGGCTTCTCCCTTCACCTACAAATTCATTTTAATAATTTCCTTTCAATTTCTCAGCCTCACAGCAGCTCAACCCAGCCCATGCAGACCTTTTTGTGGTAACATTCCGATTAACTACCCTTTCGGCATTGATGAAGGCTGTGGCAGCCCTTATTACAGTCACCTTCTTGTCTGCTCTGAGCCGGGTAAACTTGAGCTCCGAACACCTTCTGGCAGATACCCTGTCAAGAGCATAAGCTACTCCGATCCTCACATTCTTGTCACCGATCCTTTCATGTGGAATTGCCTAGACGGTGACAGTTTTCGACCAACTAGGCCTTTCAGCCTTGATACTAGTACACATTTGTCACTCTCTTCTCAAAATGACTACCTTTTCTTCAATTGTAGTGAAGAAAATGTGATCGTTCAGCCAAAGCCTATGTTCTGTGAAAGGTACCCCGAAAAGTGTGACTCATCATGTGACAGTTCTAGTTATCTTTGCAGGCACTTACCTGAGTGTGCCTCAGCATTGAAAAGAAGTACTTGCTGTTCTTACTATCCCAAAGCCACTGAATCCCTAAGGTTGATGTTGAAGTACTGTGCTAGTTATACTAGTGTGCATTGGAGAAACACCGGTGAAACACAGCCTGATGATCAAGTCCCCGAATATGGGATTCGGGTGGATTTTGATATTCCAGTTACCACTCGTTGCCTTCACTGCCAGGACACATCCAAAGGTGGTGGAACTTGTGGATTCGACACCAAAACACAGAGTTTCTTGTGCCTATGTGAGAGAGGAAATGTGACTACTTATTGTAATGGTAAGTATGAGGCATTTGTATCAATTCTTAATGTTTTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

282

Amino Acids

31.74

Weight (kDa)

5.87

Isoelectric Point (pI)

50.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 92 4.4e-11 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 441
AccB1I GGYRCC 1 cut(s) 441
AclWI GGATC 2 cut(s) 248, 269
AcsI RAATTY 1 cut(s) 32
AfaI GTAC 4 cut(s) 346, 443, 529, 584
AfiI CCNNNNNNNGG 3 cut(s) 104, 656, 657
AgeI ACCGGT 1 cut(s) 620
AgsI TTSAA 4 cut(s) 56, 388, 520, 580
AhlI ACTAGT 2 cut(s) 341, 599
AjnI CCWGG 1 cut(s) 708
AluBI AGCT 3 cut(s) 77, 205, 246
AluI AGCT 3 cut(s) 77, 205, 246
Alw21I GWGCWC 1 cut(s) 207
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 2 cut(s) 248, 269
AoxI GGCC 1 cut(s) 323
ApeKI GCWGC 2 cut(s) 74, 153
ApoI RAATTY 1 cut(s) 32
AsiGI ACCGGT 1 cut(s) 620
Asp700I GAANNNNTTC 1 cut(s) 214
Asp718I GGTACC 1 cut(s) 441
AsuC2I CCSGG 1 cut(s) 192
AsuHPI GGTGA 5 cut(s) 16, 161, 262, 314, 635
AxyI CCTNAGG 1 cut(s) 566
BanI GGYRCC 1 cut(s) 441
BanII GRGCYC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 207
BbvCI CCTCAGC 1 cut(s) 511
BbvI GCAGC 2 cut(s) 86, 165
BciT130I CCWGG 1 cut(s) 710
BclI TGATCA 1 cut(s) 640
BcnI CCSGG 1 cut(s) 192
BcoDI GTCTC 1 cut(s) 9
BcuI ACTAGT 2 cut(s) 341, 599
BfaI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
BisI GCNGC 2 cut(s) 75, 154
BlsI GCNGC 2 cut(s) 76, 155
BmcAI AGTACT 2 cut(s) 529, 584
Bme1390I CCNGG 2 cut(s) 192, 710
BmiI GGNNCC 1 cut(s) 443
BmrFI CCNGG 2 cut(s) 192, 710
BplI GAGNNNNNCTC 2 cut(s) 494, 526
Bpu10I CCTNAGC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 221
BpuMI CCSGG 1 cut(s) 192
BsaWI WCCGGW 1 cut(s) 620
BsaXI ACNNNNNCTCC 2 cut(s) 189, 219
Bsc4I CCNNNNNNNGG 3 cut(s) 104, 656, 657
Bse118I RCCGGY 1 cut(s) 620
Bse1I ACTGG 1 cut(s) 683
Bse21I CCTNAGG 1 cut(s) 566
BseBI CCWGG 1 cut(s) 710
BseGI GGATG 1 cut(s) 716
BseLI CCNNNNNNNGG 3 cut(s) 104, 656, 657
BseMII CTCAG 4 cut(s) 76, 177, 492, 525
BseNI ACTGG 1 cut(s) 683
BseXI GCAGC 2 cut(s) 86, 165
BseYI CCCAGC 1 cut(s) 83
BshFI GGCC 1 cut(s) 325
BshNI GGYRCC 1 cut(s) 441
BshTI ACCGGT 1 cut(s) 620
BsiHKAI GWGCWC 1 cut(s) 207
BsiSI CCGG 2 cut(s) 191, 621
BslFI GGGAC 1 cut(s) 632
BslI CCNNNNNNNGG 3 cut(s) 104, 656, 657
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 632
BsnI GGCC 1 cut(s) 325
Bsp1286I GDGCHC 1 cut(s) 207
Bsp143I GATC 4 cut(s) 253, 274, 408, 640
BspANI GGCC 1 cut(s) 325
BspCNI CTCAG 4 cut(s) 75, 178, 493, 524
BspLI GGNNCC 1 cut(s) 443
BspPI GGATC 2 cut(s) 248, 269
BspT107I GGYRCC 1 cut(s) 441
BsrFI RCCGGY 1 cut(s) 620
BsrI ACTGG 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 620
BssMI GATC 4 cut(s) 253, 274, 408, 640
Bst2UI CCWGG 1 cut(s) 710
Bst4CI ACNGT 5 cut(s) 167, 302, 308, 473, 587
Bst6I CTCTTC 1 cut(s) 367
BstC8I GCNNGC 1 cut(s) 491
BstDEI CTNAG 5 cut(s) 62, 186, 501, 511, 566
BstF5I GGATG 1 cut(s) 716
BstKTI GATC 4 cut(s) 256, 277, 411, 643
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 4 cut(s) 253, 274, 408, 640
BstMWI GCNNNNNNNGC 2 cut(s) 153, 705
BstNI CCWGG 1 cut(s) 710
BstSCI CCNGG 2 cut(s) 190, 708
BstV1I GCAGC 2 cut(s) 86, 165
Bsu36I CCTNAGG 1 cut(s) 566
BsuRI GGCC 1 cut(s) 325
BtsCI GGATG 1 cut(s) 716
BtsI GCAGTG 1 cut(s) 703
BtsIMutI CAGTG 2 cut(s) 555, 703
Cac8I GCNNGC 1 cut(s) 491
Cfr10I RCCGGY 1 cut(s) 620
Csp6I GTAC 4 cut(s) 345, 442, 528, 583
CspAI ACCGGT 1 cut(s) 620
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviAII CATG 3 cut(s) 90, 283, 465
CviQI GTAC 4 cut(s) 345, 442, 528, 583
DdeI CTNAG 5 cut(s) 62, 186, 501, 511, 566
DpnI GATC 4 cut(s) 255, 276, 410, 642
DpnII GATC 4 cut(s) 253, 274, 408, 640
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 325
Eco24I GRGCYC 1 cut(s) 207
Eco53kI GAGCTC 1 cut(s) 205
Eco81I CCTNAGG 1 cut(s) 566
EcoICRI GAGCTC 1 cut(s) 205
EcoRII CCWGG 1 cut(s) 708
EcoT38I GRGCYC 1 cut(s) 207
FaeI CATG 3 cut(s) 93, 286, 468
FalI AAGNNNNNCTT 2 cut(s) 514, 546
FaqI GGGAC 1 cut(s) 632
FatI CATG 3 cut(s) 89, 282, 464
FbaI TGATCA 1 cut(s) 640
Fnu4HI GCNGC 2 cut(s) 75, 154
FokI GGATG 1 cut(s) 703
FriOI GRGCYC 1 cut(s) 207
Fsp4HI GCNGC 2 cut(s) 75, 154
FspBI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
GluI GCNGC 2 cut(s) 75, 154
GsaI CCCAGC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 325
HapII CCGG 2 cut(s) 191, 621
Hin1II CATG 3 cut(s) 93, 286, 468
HinfI GANTC 4 cut(s) 458, 560, 661, 741
HpaII CCGG 2 cut(s) 191, 621
HphI GGTGA 5 cut(s) 16, 161, 262, 314, 635
Hpy166II GTNNAC 2 cut(s) 197, 347
Hpy188I TCNGA 4 cut(s) 117, 187, 209, 253
Hpy188III TCNNGA 1 cut(s) 235
Hpy8I GTNNAC 2 cut(s) 197, 347
HpyAV CCTTC 5 cut(s) 31, 137, 182, 225, 710
HpyCH4III ACNGT 5 cut(s) 167, 302, 308, 473, 587
HpyCH4V TGCA 3 cut(s) 93, 489, 607
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 705
HpyF3I CTNAG 5 cut(s) 62, 186, 501, 511, 566
Hsp92II CATG 3 cut(s) 93, 286, 468
KpnI GGTACC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 640
Kzo9I GATC 4 cut(s) 253, 274, 408, 640
LmnI GCTCC 1 cut(s) 210
Lsp1109I GCAGC 2 cut(s) 86, 165
MaeI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
MaeIII GTNAC 9 cut(s) 107, 167, 268, 302, 354, 455, 467, 685, 790
MalI GATC 4 cut(s) 255, 276, 410, 642
MboI GATC 4 cut(s) 253, 274, 408, 640
MboII GAAGA 3 cut(s) 354, 376, 410
MfeI CAATTG 1 cut(s) 388
MhlI GDGCHC 1 cut(s) 207
MluCI AATT 6 cut(s) 32, 45, 56, 289, 388, 831
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 77, 267, 520, 776, 811
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 3 cut(s) 41, 120, 837
MspI CCGG 2 cut(s) 191, 621
MspR9I CCNGG 2 cut(s) 192, 710
MunI CAATTG 1 cut(s) 388
MvaI CCWGG 1 cut(s) 710
MwoI GCNNNNNNNGC 2 cut(s) 153, 705
NciI CCSGG 1 cut(s) 192
NdeII GATC 4 cut(s) 253, 274, 408, 640
NlaIII CATG 3 cut(s) 93, 286, 468
NlaIV GGNNCC 1 cut(s) 443
NmuCI GTSAC 7 cut(s) 167, 268, 302, 354, 455, 467, 790
PceI AGGCCT 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 3 cut(s) 560, 661, 741
PinAI ACCGGT 1 cut(s) 620
PkrI GCNGC 2 cut(s) 76, 155
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
Psp124BI GAGCTC 1 cut(s) 207
Psp6I CCWGG 1 cut(s) 708
PspFI CCCAGC 1 cut(s) 83
PspGI CCWGG 1 cut(s) 708
PspN4I GGNNCC 1 cut(s) 443
PsrI GAACNNNNNNTAC 2 cut(s) 520, 552
RsaI GTAC 4 cut(s) 346, 443, 529, 584
RsaNI GTAC 4 cut(s) 345, 442, 528, 583
SacI GAGCTC 1 cut(s) 207
SaqAI TTAA 3 cut(s) 41, 120, 837
SatI GCNGC 2 cut(s) 75, 154
Sau3AI GATC 4 cut(s) 253, 274, 408, 640
ScaI AGTACT 2 cut(s) 529, 584
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 2 cut(s) 192, 710
SduI GDGCHC 1 cut(s) 207
SgrAI CRCCGGYG 1 cut(s) 620
SmlI CTYRAG 1 cut(s) 200
SmoI CTYRAG 1 cut(s) 200
SpeI ACTAGT 2 cut(s) 341, 599
Sse9I AATT 6 cut(s) 32, 45, 56, 289, 388, 831
SseBI AGGCCT 1 cut(s) 325
SspMI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
SstI GAGCTC 1 cut(s) 207
StuI AGGCCT 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 190, 708
TaaI ACNGT 5 cut(s) 167, 302, 308, 473, 587
TaqI TCGA 2 cut(s) 313, 744
TasI AATT 6 cut(s) 32, 45, 56, 289, 388, 831
TatI WGTACW 3 cut(s) 344, 527, 582
TfiI GAWTC 3 cut(s) 560, 661, 741
Tru1I TTAA 3 cut(s) 41, 120, 837
Tru9I TTAA 3 cut(s) 41, 120, 837
TscAI CASTG 2 cut(s) 562, 710
TseFI GTSAC 7 cut(s) 167, 268, 302, 354, 455, 467, 790
TseI GCWGC 2 cut(s) 74, 153
Tsp45I GTSAC 7 cut(s) 167, 268, 302, 354, 455, 467, 790
TspDTI ATGAA 3 cut(s) 25, 156, 271
TspRI CASTG 2 cut(s) 562, 710
XapI RAATTY 1 cut(s) 32
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 6 cut(s) 296, 321, 342, 477, 591, 600
ZrmI AGTACT 2 cut(s) 529, 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.