MD05G1349900.v1.1

Wall-associated receptor kinase galacturonan-binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010019
Physical Location & Seq
Forward (+)
46777070 .. 46778705
1636 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1349900.v1.1.491

Sequence Viewer

Length: 978 bp
ATGTCTCCTCTAACAACTCCTATGCCGTTTGTCCTGCTCATTTTCCTTACCCTTCCGTTTGTCAGCCTCACGGCACCTCAAGCCAGCCTCTGCAGAACTTCTTGCGGCAACATTCCCATCAACTACCCTTTCGGCATCGATGAGGGCTGTGGGAGTCCTTACTACAAAAACATCCTCGTATGCTCCGAATCCTCTAAACTCGAACTCCGAACACCTTCCGGAAGATACCCCGTTAAATCCATAAGCTACTCCGACCCTCACATTCTTCTCATCGATCCTTTCATGTGGAATTGCCTAGACGGTGACACATTCCGAGCCACAAGACCTTTTAGCCTCGACACGAGCACGCATTTATCTCTCTCTCCTCAAAACGACTACCTATTCTTTAATTGTAGTGAACAAAATGTGATCATTGAGCCAAAGCCTGTGTTTTGTGAGAGGTTTCCGGAGCGATGTGACTCTTCGTGTGACAGTTCTAGTTATCTTTGCAGGCACTTGCCTGAGTGCGCCTCAGCGTTGAGGACAAGTTCTTGCTGCTCTTACTATCCCAAAGCCACTGAGTCTCTGAGGTTGATGTTGAAGTATTGTGCTACTTATACTAGTGTGTATTGGAGAAGCAGTGGTGAAAATCAGCCGGATGATCAAGTTCCTGAATACGGAATTCGTGTCAATTTCGATATTCCGGTGACCACCCGTTGCCTTCAGTGTCAGGACACATCTAAAGGAGGCGGGACATGTGGATTTGACACGAAAACACTCAACTTCTGGTGCTTATGTGATAGAGGAAATGTCACTACGTATTGTAAAGATCACAGCATTTCTCAGAATAGGAGGAATGGAGTACTTGCAGGGACGATTAGTGGAGTGACAGTTGCAGGAGCCTTTGGAGTTGGAGCAGGAATTTGGTACTTGAAGAAAATGAGGGCTAAATCACCAGTAACATGTGGGGTTCAGAGCAATGAGAATAGGCTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind

Protein Analysis

326

Amino Acids

36.15

Weight (kDa)

7.74

Isoelectric Point (pI)

56.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 31 - 90 3.8e-12 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 73
AccIII TCCGGA 2 cut(s) 218, 445
AciI CCGC 2 cut(s) 105, 729
AclWI GGATC 1 cut(s) 269
AcsI RAATTY 2 cut(s) 660, 900
AcuI CTGAAG 1 cut(s) 686
AfaI GTAC 2 cut(s) 843, 908
AfiI CCNNNNNNNGG 1 cut(s) 656
AflIII ACRYGT 2 cut(s) 734, 941
AgsI TTSAA 2 cut(s) 580, 913
AhlI ACTAGT 1 cut(s) 599
AluBI AGCT 1 cut(s) 246
AluI AGCT 1 cut(s) 246
Alw21I GWGCWC 1 cut(s) 347
Alw26I GTCTC 2 cut(s) 9, 567
AlwI GGATC 1 cut(s) 269
AlwNI CAGNNNCTG 1 cut(s) 90
Aor13HI TCCGGA 2 cut(s) 218, 445
ApeKI GCWGC 1 cut(s) 534
ApoI RAATTY 2 cut(s) 660, 900
Asp700I GAANNNNTTC 1 cut(s) 214
AspLEI GCGC 1 cut(s) 509
AsuHPI GGTGA 4 cut(s) 314, 635, 697, 924
BanI GGYRCC 1 cut(s) 73
BauI CACGAG 1 cut(s) 340
Bbv12I GWGCWC 1 cut(s) 347
BbvCI CCTCAGC 1 cut(s) 511
BbvI GCAGC 1 cut(s) 521
BccI CCATC 1 cut(s) 125
BceAI ACGGC 2 cut(s) 10, 87
BclI TGATCA 2 cut(s) 408, 640
BcoDI GTCTC 2 cut(s) 9, 567
BcuI ACTAGT 1 cut(s) 599
BfaI CTAG 3 cut(s) 296, 477, 600
BfmI CTRYAG 1 cut(s) 91
BisI GCNGC 2 cut(s) 106, 535
BlsI GCNGC 2 cut(s) 107, 536
BmcAI AGTACT 1 cut(s) 843
BmiI GGNNCC 2 cut(s) 75, 880
BmsI GCATC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 494, 526
Bpu10I CCTNAGC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 63
Bsa29I ATCGAT 2 cut(s) 138, 273
BsaAI YACGTR 1 cut(s) 798
BsaWI WCCGGW 3 cut(s) 218, 445, 682
BsaXI ACNNNNNCTCC 6 cut(s) 189, 219, 604, 634, 855, 885
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse1I ACTGG 1 cut(s) 935
Bse3DI GCAATG 1 cut(s) 964
BseAI TCCGGA 2 cut(s) 218, 445
BseCI ATCGAT 2 cut(s) 138, 273
BseGI GGATG 2 cut(s) 171, 643
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMI GCAATG 1 cut(s) 964
BseMII CTCAG 5 cut(s) 492, 525, 549, 557, 836
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 1 cut(s) 354
BseXI GCAGC 1 cut(s) 521
BshNI GGYRCC 1 cut(s) 73
BshVI ATCGAT 2 cut(s) 138, 273
BsiHKAI GWGCWC 1 cut(s) 347
BsiSI CCGG 4 cut(s) 219, 446, 635, 683
BslFI GGGAC 2 cut(s) 745, 865
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 2 cut(s) 9, 567
BsmFI GGGAC 2 cut(s) 745, 865
Bsp1286I GDGCHC 1 cut(s) 347
Bsp13I TCCGGA 2 cut(s) 218, 445
Bsp143I GATC 4 cut(s) 274, 408, 640, 808
BspACI CCGC 2 cut(s) 105, 729
BspCNI CTCAG 5 cut(s) 493, 524, 550, 558, 835
BspDI ATCGAT 2 cut(s) 138, 273
BspEI TCCGGA 2 cut(s) 218, 445
BspLI GGNNCC 2 cut(s) 75, 880
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 269
BspT107I GGYRCC 1 cut(s) 73
BsrDI GCAATG 1 cut(s) 964
BsrI ACTGG 1 cut(s) 935
BssMI GATC 4 cut(s) 274, 408, 640, 808
BssSI CACGAG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 340
Bst4CI ACNGT 3 cut(s) 302, 473, 871
Bst6I CTCTTC 1 cut(s) 466
BstBAI YACGTR 1 cut(s) 798
BstC8I GCNNGC 3 cut(s) 85, 347, 491
BstDEI CTNAG 5 cut(s) 501, 511, 558, 566, 822
BstEII GGTNACC 1 cut(s) 685
BstF5I GGATG 2 cut(s) 171, 643
BstHHI GCGC 1 cut(s) 509
BstKTI GATC 4 cut(s) 277, 411, 643, 811
BstMAI GTCTC 2 cut(s) 9, 567
BstMBI GATC 4 cut(s) 274, 408, 640, 808
BstMWI GCNNNNNNNGC 1 cut(s) 80
BstNSI RCATGY 2 cut(s) 738, 945
BstPI GGTNACC 1 cut(s) 685
BstSFI CTRYAG 1 cut(s) 91
BstSNI TACGTA 1 cut(s) 798
BstV1I GCAGC 1 cut(s) 521
Bsu15I ATCGAT 2 cut(s) 138, 273
BsuTUI ATCGAT 2 cut(s) 138, 273
BtgZI GCGATG 1 cut(s) 466
BtsCI GGATG 2 cut(s) 171, 643
BtsI GCAGTG 1 cut(s) 625
BtsIMutI CAGTG 3 cut(s) 555, 625, 710
Cac8I GCNNGC 3 cut(s) 85, 347, 491
CaiI CAGNNNCTG 1 cut(s) 90
CfoI GCGC 1 cut(s) 509
ClaI ATCGAT 2 cut(s) 138, 273
Csp6I GTAC 2 cut(s) 842, 907
CviAII CATG 3 cut(s) 283, 735, 942
CviQI GTAC 2 cut(s) 842, 907
DdeI CTNAG 5 cut(s) 501, 511, 558, 566, 822
DpnI GATC 4 cut(s) 276, 410, 642, 810
DpnII GATC 4 cut(s) 274, 408, 640, 808
Eam1104I CTCTTC 1 cut(s) 466
EarI CTCTTC 1 cut(s) 466
Eco105I TACGTA 1 cut(s) 798
Eco57I CTGAAG 1 cut(s) 686
Eco91I GGTNACC 1 cut(s) 685
EcoO65I GGTNACC 1 cut(s) 685
EcoRI GAATTC 1 cut(s) 660
FaeI CATG 3 cut(s) 286, 738, 945
FaiI YATR 8 cut(s) 23, 181, 242, 284, 597, 736, 775, 943
FaqI GGGAC 2 cut(s) 745, 865
FatI CATG 3 cut(s) 282, 734, 941
FauI CCCGC 1 cut(s) 722
FbaI TGATCA 2 cut(s) 408, 640
Fnu4HI GCNGC 2 cut(s) 106, 535
FokI GGATG 2 cut(s) 158, 650
Fsp4HI GCNGC 2 cut(s) 106, 535
FspBI CTAG 3 cut(s) 296, 477, 600
GlaI GCGC 1 cut(s) 508
GluI GCNGC 2 cut(s) 106, 535
HapII CCGG 4 cut(s) 219, 446, 635, 683
HhaI GCGC 1 cut(s) 509
Hin1II CATG 3 cut(s) 286, 738, 945
Hin6I GCGC 1 cut(s) 507
HinP1I GCGC 1 cut(s) 507
HinfI GANTC 4 cut(s) 154, 188, 458, 560
HpaII CCGG 4 cut(s) 219, 446, 635, 683
HphI GGTGA 4 cut(s) 314, 635, 697, 924
Hpy166II GTNNAC 1 cut(s) 398
Hpy188I TCNGA 7 cut(s) 187, 209, 253, 314, 567, 825, 954
Hpy188III TCNNGA 4 cut(s) 219, 446, 650, 710
Hpy8I GTNNAC 1 cut(s) 398
HpyAV CCTTC 3 cut(s) 62, 225, 710
HpyCH4III ACNGT 3 cut(s) 302, 473, 871
HpyCH4IV ACGT 1 cut(s) 797
HpyCH4V TGCA 4 cut(s) 93, 489, 848, 875
HpyF10VI GCNNNNNNNGC 1 cut(s) 80
HpyF3I CTNAG 5 cut(s) 501, 511, 558, 566, 822
HpySE526I ACGT 1 cut(s) 797
Hsp92II CATG 3 cut(s) 286, 738, 945
HspAI GCGC 1 cut(s) 507
Kpn2I TCCGGA 2 cut(s) 218, 445
Ksp22I TGATCA 2 cut(s) 408, 640
Kzo9I GATC 4 cut(s) 274, 408, 640, 808
LmnI GCTCC 4 cut(s) 188, 448, 878, 893
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 144
MaeI CTAG 3 cut(s) 296, 477, 600
MaeII ACGT 1 cut(s) 797
MaeIII GTNAC 7 cut(s) 302, 455, 467, 685, 790, 865, 937
MalI GATC 4 cut(s) 276, 410, 642, 810
MboI GATC 4 cut(s) 274, 408, 640, 808
MboII GAAGA 4 cut(s) 234, 257, 453, 925
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 5 cut(s) 289, 388, 660, 670, 900
MlyI GAGTC 3 cut(s) 163, 452, 569
MmeI TCCRAC 2 cut(s) 276, 871
MroI TCCGGA 2 cut(s) 218, 445
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 2 cut(s) 234, 387
MspI CCGG 4 cut(s) 219, 446, 635, 683
MwoI GCNNNNNNNGC 1 cut(s) 80
NdeII GATC 4 cut(s) 274, 408, 640, 808
NlaIII CATG 3 cut(s) 286, 738, 945
NlaIV GGNNCC 2 cut(s) 75, 880
NmuCI GTSAC 6 cut(s) 302, 455, 467, 685, 790, 865
NspI RCATGY 2 cut(s) 738, 945
PciI ACATGT 2 cut(s) 734, 941
PcsI WCGNNNNNNNCGW 1 cut(s) 183
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 1 cut(s) 188
PkrI GCNGC 2 cut(s) 107, 536
PleI GAGTC 3 cut(s) 162, 452, 568
PpsI GAGTC 3 cut(s) 162, 452, 568
Ppu21I YACGTR 1 cut(s) 798
PscI ACATGT 2 cut(s) 734, 941
PspEI GGTNACC 1 cut(s) 685
PspN4I GGNNCC 2 cut(s) 75, 880
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 90
RsaI GTAC 2 cut(s) 843, 908
RsaNI GTAC 2 cut(s) 842, 907
SaqAI TTAA 2 cut(s) 234, 387
SatI GCNGC 2 cut(s) 106, 535
Sau3AI GATC 4 cut(s) 274, 408, 640, 808
ScaI AGTACT 1 cut(s) 843
SchI GAGTC 3 cut(s) 163, 452, 569
SduI GDGCHC 1 cut(s) 347
SetI ASST 8 cut(s) 79, 217, 248, 328, 381, 443, 572, 800
SfaNI GCATC 1 cut(s) 144
SfcI CTRYAG 1 cut(s) 91
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
SnaBI TACGTA 1 cut(s) 798
SpeI ACTAGT 1 cut(s) 599
Sse9I AATT 5 cut(s) 289, 388, 660, 670, 900
SsiI CCGC 2 cut(s) 105, 729
SspMI CTAG 3 cut(s) 296, 477, 600
TaaI ACNGT 3 cut(s) 302, 473, 871
TaiI ACGT 1 cut(s) 800
TaqI TCGA 5 cut(s) 138, 201, 273, 336, 675
TasI AATT 5 cut(s) 289, 388, 660, 670, 900
TatI WGTACW 1 cut(s) 841
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 1 cut(s) 188
Tru1I TTAA 2 cut(s) 234, 387
Tru9I TTAA 2 cut(s) 234, 387
TscAI CASTG 3 cut(s) 562, 625, 710
TseFI GTSAC 6 cut(s) 302, 455, 467, 685, 790, 865
TseI GCWGC 1 cut(s) 534
Tsp45I GTSAC 6 cut(s) 302, 455, 467, 685, 790, 865
TspDTI ATGAA 1 cut(s) 271
TspGWI ACGGA 2 cut(s) 45, 672
TspRI CASTG 3 cut(s) 562, 625, 710
XapI RAATTY 2 cut(s) 660, 900
XceI RCATGY 2 cut(s) 738, 945
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 3 cut(s) 296, 477, 600
ZrmI AGTACT 1 cut(s) 843
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.