Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Reverse (-)

50277628 .. 50286493

Gene Structure 8866 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1206400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3780 bp

ATGTCTTCATTGGATGATAATATTGATGAGCTATCAGTCATGCCAATGATTAGGTTTTCTGAATGGTTAGATGAGTTCTTGTGTCGTCTATTTTCCTTACTCTTACACCTGGAACCAAGCAGTGTTACGAATGAAGGCCTACATTCATCAGCAGTGTCAGGAACTTTTCTAGTTGAGGATGGTCCTTACTACTATTGCATGCTTGAAATCTTGCTCGGGAGACTTTCACAACCTCTATATAATCAGGCTTTGAAGAAGATCTCCAAGTTTGTGAAGACAAATATTCTTCCTGGGGCAATTTCAGAGGTTGGACTGCTTTGTTGTGCATGCGTTCATTCCAACCCAGAAGAAGCGGTTACTCAACTTGTCAAGCCAGTTTTATTGTCTGTTATTTCTTCTTTGGAAGGAACACCAGCTACAGGATTTGGAGGTAGAGGAATATGTGACACTTCAGTGTCAACTAAGGTTAAGCCAACAATTTCTCCAGCTCTTGAAACAGCAATTGACTATCAATTAAAAGTGGTATCAGTTGCCATAAGTTATGGAGGTCCTGCACTTCTGCGCTACAAGGATCACTTCAAAGAGGCTATACTTTCTGCTTTTGAGTCCCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGATCTCTCCTTGGAAGCCTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTGTTTTGCATCATCGAAATGCTGCTGCATTAGAGGAATGGATCAGCACCAAAGATTACTTGAATGATAAACCATTGGCGGCCCCCAAGTGGCATATCCCAAGTGTCGAAGAAGTTGAATTTGCAAATGAACTCTTGGACTTTCATTTTCGGATGGCTTTGGATGATCTTTCGAGAATATGTGAAACTAAACTCCACTCTGATCCAGGAGATGAGAAAGATCACTTGAAAGTGACTCTTCTACGTATAGATTCTTCATTGCAAGGTGTTTTGTCTTGTTTGCCTGACTTCGCCCCGTCCTCAAGGAATGGGACTGTTGAACATCCAAATCATGCTGCTTTTCTAATAGCTGGAGCAACAGGTTCAACTGTTGGTACCACTAAATTGCGGGAAAAAGCTGCTGACATTATACATGCAGCCTGCAAATACATACTAGAGAAAAAATCAGATGATAGCATTCTGTTGATACTCATTATTCGTATTATGGATGCTTTAGGAAACTATGGAAGTTCGGAATATGATGAGTGGTCAAATCACAGGCAGGCATGGAAACTAGAATCTGCTGCCATTGTAGAACCTTCAGTAAACTTTATAGTTTCAGCTCATTCTAAGGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCATTCATGCACAGTACTTGGAGATCATCACAATCATCTTATCACATATATCGTACTAATGGGAATTTCGGTCCACCAGACCATGTGAATCTTTTGGTGGATAATCTTCTAAATCTCTCATTGCATAGCTATGCAACTGTTCGTTTACTTGCTGGAAAATCTCTTTTAAAGATGATAAAGAGATGGCCATCCATGATTTCAAAGTGCGTCCTTTCTCTAACAGAGAATTTACGGAATCCCAAGTCACCAGAATATGCGGTGCTAGGTTCATGTGCAGTCCTTGCCACGCAGACAGTTCTCAAGCATTTGACAATGGACCCAAAGGCGTTCTCTTCTTGTATTCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAAACCCAGAAAGCAATCAATGAGCTTTTTGTCAAATACAACATCCACTTTGCTGGAGTATCTAGAAGCATTTTCAGGGCATCAGGCAATCATACAGAAGCACCAGATTTTGCAGATTTGGTTTCTCAGATCACTTCTATGAGTTTTGAATCTACCGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTCTACTCTTGTTGGCTATGTCATCTAGGAATGATCCAACTTCCTCCTCAAAAATTTTGAGTGAAACTGCAGGTCACTTCTTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTTGCAATTTCAGCTTTAAATACACTGTTAAAAGAATCACCTTATAAACTGTCTGCTGGGGAACAGACTCTTCCCTCTGGGAATTTGCAAGGAAGTAAAAAGTCATCCCTTGAAGGAGAATTAACGCAAATATTTCAGGAAGATGGTTTTTTCAATGAGACCTTGAACAGTCTTTCTCATGTTCATATTGTTACTGATGCTGAAAGTGCATCTTCTAGAGGAAATCATGGATCTTTTCAGAGCCTAGCAGACAAATCAATCACCCGATTTTATTTTGATTTCACCGCTTCATGGCCACGCACTCCTACTTGGATCTCTTTATTAGGGAGTGACACATTTTACTCAAACTTTGCGAGAATTTTCAAGCGGCTAATACAGGAATGTGGCATACCTGTTCTATTGTCACTGAAAAGCTCATTAGAGGAGTTTGTAAATGCCAAGGAGAGGTCTAAGCAGTGTGTTGCTGCTGAAGCATTGGCTGGAATATTGCATTCTGATGTTGACGGTATTTTAGTGGCATGGGACACTTGGATTTTGGCCCAGTTGCAGAATATCATCGTATCACAATCAGTAGAGTCAATACCTGAGTGGGCAGCCTGTATACGTTACGCTGTTACTGGGAAAGGGAAGCATGGAACGAGAGTTCCTGTTTTGAGGCAACCAGTACTGGATTGTTTGGCCACACCTTTAACTGGAACTGTAACCACGACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCGGCTGCACTTATAGAATTATCTCCACAGACAATGCCGTTCACTGAAGTACAGCTCCATTATAGACTTCTGGAGGAGCTGCTTAGTAGCATGTGCCATTCATCTGCCCAGGTAAGAGAAGCTTTAGGTGTTACTCTTGCAGTGTTGTGCTCTAACATCCAGCTTTATGAGTCATTTAATCATGAGCATTCACATGAGGGGGAAAAAAGAGATGTTGCCTACAAATTTGATGGGAAAAGTTGGGTTCAGTTTCTGAAAGAGAGAGCATCTGAAGTGCTGATGAATATTCAGAATACTTCCCAGTCTGACAGTTTGGAGACCGCAACAAATACTAGTTCTGAGAATGGGAATTCCAATAGTGATTCACAGGATGATATCAAGTGGATGGAGACGCTTTTCCATTTTATCATCTCATCTTTGAAGTCTGGGCGAATTTCATATCTTCTGGATGTTATTGTGGGACTTCTTTCTCCTGTAATATCCTTACAGGAAACATCAAGTAAAGATCTGTCAACCTTAGCGAAAGTAGCTTTTGAATTGCTGAAGTGGAGAATTTTCTGTGGACCTCATCTCCAGGAGGCTGTATCTGTGATCCTTTCTTCTGCCAATGACTCTAACTGGCGAATTAGATCTGCAACACTTACATATCTGCGAACTTTTATGTATAGGCACAGTTTCGTTCTCTCAAATGCGAAGAAGCAGCAAATTTGGAGAACTGTTGAAAATCTACTTTTAGACAACCAAGTAAGAGAGCATGCTGCTGCAGTTTTAGCGGGCCTAATGAAGGGTGGGGATGAAGATCTTGCTAAAGACTTTCGCGATAAAGCTTATACAGAAGCTACTATTCTTCAGAGGAAGAGAAAGCGGAGAACTTTGAGCTCAAGCCAGTCCATAGCTTCTATTCACGGTGCTGTACTTGCTTTGGTAGCTTCCGTGTTGTCAGCCCCGTATGATATGCCCAGGTACTTGGTTGCCCGAGCACGTTACTTTACTTGCTCGTTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1260

Amino Acids

139.82

Weight (kDa)

7.26

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	8 - 323	5.1e-19	Proteasome activator complex subunit 4, mid HEAT repeats region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2082
Acc36I	ACCTGC	1 cut(s)	1979
Acc65I	GGTACC	1 cut(s)	1073
AccB1I	GGYRCC	1 cut(s)	1073
AccI	GTMKAC	1 cut(s)	2638
AccII	CGCG	2 cut(s)	1327, 3594
AclWI	GGATC	7 cut(s)	579, 749, 896, 1946, 2275, 2357, 3361
AcoI	YGGCCR	3 cut(s)	1532, 2330, 2715
AcuI	CTGAAG	7 cut(s)	435, 1263, 2526, 2841, 3066, 3338, 3608
AfaI	GTAC	9 cut(s)	695, 1075, 1363, 1402, 1898, 2703, 2826, 3690, 3740
AfeI	AGCGCT	1 cut(s)	2762
AfiI	CCNNNNNNNGG	7 cut(s)	419, 615, 789, 2328, 2481, 2729, 3559
AhlI	ACTAGT	1 cut(s)	3107
AjnI	CCWGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
AleI	CACNNNNGTG	1 cut(s)	452
AloI	GAACNNNNNNTCC	6 cut(s)	654, 686, 2664, 2665, 2696, 2697
Alw21I	GWGCWC	3 cut(s)	2927, 3656, 3757
Alw26I	GTCTC	4 cut(s)	214, 2189, 3086, 3158
AlwI	GGATC	7 cut(s)	579, 749, 896, 1946, 2275, 2357, 3361
AlwNI	CAGNNNCTG	1 cut(s)	3028
Ama87I	CYCGRG	2 cut(s)	215, 3750
Aor51HI	AGCGCT	1 cut(s)	2762
AoxI	GGCC	8 cut(s)	136, 780, 1322, 1532, 2330, 2574, 2715, 3550
Asp700I	GAANNNNTTC	1 cut(s)	1699
Asp718I	GGTACC	1 cut(s)	1073
AspLEI	GCGC	2 cut(s)	564, 2763
AspS9I	GGNCC	8 cut(s)	182, 548, 781, 1418, 1663, 2575, 3338, 3550
AsuHPI	GGTGA	5 cut(s)	647, 1584, 2067, 2290, 2311
AvaI	CYCGRG	2 cut(s)	215, 3750
AvaII	GGWCC	5 cut(s)	182, 548, 1418, 1663, 3338
BaeGI	GKGCMC	1 cut(s)	1336
BalI	TGGCCA	3 cut(s)	1534, 2332, 2717
BanI	GGYRCC	1 cut(s)	1073
BanII	GRGCYC	1 cut(s)	3656
BarI	GAAGNNNNNNTAC	2 cut(s)	937, 969
BbsI	GAAGAC	1 cut(s)	281
Bbv12I	GWGCWC	3 cut(s)	2927, 3656, 3757
BccI	CCATC	9 cut(s)	173, 619, 847, 1524, 1543, 1900, 2174, 2999, 3154
BceAI	ACGGC	1 cut(s)	2797
BciT130I	CCWGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
BciVI	GTATCC	1 cut(s)	2028
BclI	TGATCA	2 cut(s)	637, 688
BcoDI	GTCTC	4 cut(s)	214, 2189, 3086, 3158
BcuI	ACTAGT	1 cut(s)	3107
BfaI	CTAG	9 cut(s)	170, 1133, 1253, 1610, 1788, 1944, 2253, 2282, 3108
BfmI	CTRYAG	4 cut(s)	417, 1986, 2748, 3537
BfoI	RGCGCY	1 cut(s)	2764
BfuAI	ACCTGC	1 cut(s)	1979
BfuI	GTATCC	1 cut(s)	2028
BglII	AGATCT	4 cut(s)	258, 3280, 3404, 3574
BmcAI	AGTACT	2 cut(s)	1363, 2703
Bme1390I	CCNGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
Bme18I	GGWCC	5 cut(s)	182, 548, 1418, 1663, 3338
BmeT110I	CYCGRG	2 cut(s)	215, 3750
BmgT120I	GGNCC	8 cut(s)	182, 548, 781, 1418, 1663, 2575, 3338, 3550
BmiI	GGNNCC	4 cut(s)	114, 783, 1075, 1665
BmrFI	CCNGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
BmrI	ACTGGG	3 cut(s)	2572, 2664, 3070
BmsI	GCATC	6 cut(s)	718, 1177, 1814, 2224, 2255, 3050
BmuI	ACTGGG	3 cut(s)	2572, 2664, 3070
BpiI	GAAGAC	1 cut(s)	281
BpmI	CTGGAG	5 cut(s)	468, 1071, 1800, 2867, 3332
Bpu10I	CCTNAGC	1 cut(s)	3292
BpuEI	CTTGAG	3 cut(s)	985, 1631, 3640
BsaAI	YACGTR	1 cut(s)	944
BsaBI	GATNNNNATC	2 cut(s)	1534, 3573
BsaI	GGTCTC	2 cut(s)	2189, 3086
BsaJI	CCNNGG	6 cut(s)	290, 658, 1325, 2475, 2883, 3734
BsaXI	ACNNNNNCTCC	4 cut(s)	466, 496, 3330, 3360
Bsc4I	CCNNNNNNNGG	7 cut(s)	419, 615, 789, 2328, 2481, 2729, 3559
Bse118I	RCCGGY	1 cut(s)	1880
Bse1I	ACTGG	9 cut(s)	374, 2578, 2659, 2699, 2709, 2734, 3076, 3398, 3661
Bse3DI	GCAATG	2 cut(s)	956, 1466
Bse8I	GATNNNNATC	2 cut(s)	1534, 3573
BseBI	CCWGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
BseDI	CCNNGG	6 cut(s)	290, 658, 1325, 2475, 2883, 3734
BseJI	GATNNNNATC	2 cut(s)	1534, 3573
BseLI	CCNNNNNNNGG	7 cut(s)	419, 615, 789, 2328, 2481, 2729, 3559
BseMI	GCAATG	2 cut(s)	956, 1466
BseMII	CTCAG	4 cut(s)	1865, 2039, 2613, 3105
BseNI	ACTGG	9 cut(s)	374, 2578, 2659, 2699, 2709, 2734, 3076, 3398, 3661
BseRI	GAGGAG	3 cut(s)	1954, 2474, 2864
BseSI	GKGCMC	1 cut(s)	1336
BseYI	CCCAGC	1 cut(s)	2093
BsgI	GTGCAG	3 cut(s)	537, 1641, 2766
Bsh1236I	CGCG	2 cut(s)	1327, 3594
BshFI	GGCC	8 cut(s)	138, 782, 1324, 1534, 2332, 2576, 2717, 3552
BshNI	GGYRCC	1 cut(s)	1073
BsiHKAI	GWGCWC	3 cut(s)	2927, 3656, 3757
BsiHKCI	CYCGRG	2 cut(s)	215, 3750
BsiSI	CCGG	1 cut(s)	1881
BslFI	GGGAC	4 cut(s)	592, 1024, 2573, 3249
BslI	CCNNNNNNNGG	7 cut(s)	419, 615, 789, 2328, 2481, 2729, 3559
BsmAI	GTCTC	4 cut(s)	214, 2189, 3086, 3158
BsmBI	CGTCTC	1 cut(s)	3158
BsmFI	GGGAC	4 cut(s)	592, 1024, 2573, 3249
BsmI	GAATGC	4 cut(s)	1155, 1349, 2527, 2962
BsnI	GGCC	8 cut(s)	138, 782, 1324, 1534, 2332, 2576, 2717, 3552
Bso31I	GGTCTC	2 cut(s)	2189, 3086
BsoBI	CYCGRG	2 cut(s)	215, 3750
Bsp1286I	GDGCHC	4 cut(s)	1336, 2927, 3656, 3757
Bsp68I	TCGCGA	1 cut(s)	3594
BspANI	GGCC	8 cut(s)	138, 782, 1324, 1534, 2332, 2576, 2717, 3552
BspCNI	CTCAG	4 cut(s)	1864, 2038, 2614, 3106
BspFNI	CGCG	2 cut(s)	1327, 3594
BspHI	TCATGA	2 cut(s)	1714, 2956
BspLI	GGNNCC	4 cut(s)	114, 783, 1075, 1665
BspMAI	CTGCAG	2 cut(s)	1990, 3541
BspMI	ACCTGC	1 cut(s)	1979
BspPI	GGATC	7 cut(s)	579, 749, 896, 1946, 2275, 2357, 3361
BspT107I	GGYRCC	1 cut(s)	1073
BspTNI	GGTCTC	2 cut(s)	2189, 3086
BsrDI	GCAATG	2 cut(s)	956, 1466
BsrFI	RCCGGY	1 cut(s)	1880
BsrI	ACTGG	9 cut(s)	374, 2578, 2659, 2699, 2709, 2734, 3076, 3398, 3661
BssAI	RCCGGY	1 cut(s)	1880
BssECI	CCNNGG	6 cut(s)	290, 658, 1325, 2475, 2883, 3734
BssNAI	GTATAC	1 cut(s)	2639
BssT1I	CCWWGG	2 cut(s)	658, 2475
Bst1107I	GTATAC	1 cut(s)	2639
Bst2UI	CCWGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
Bst6I	CTCTTC	5 cut(s)	942, 1310, 1684, 2112, 3626
BstAPI	GCANNNNNTGC	1 cut(s)	1628
BstBAI	YACGTR	1 cut(s)	944
BstC8I	GCNNGC	7 cut(s)	200, 328, 1120, 1242, 1886, 3531, 3550
BstDEI	CTNAG	9 cut(s)	462, 1308, 1851, 2025, 2487, 2622, 2858, 3114, 3292
BstDSI	CCRYGG	1 cut(s)	1325
BstENI	CCTNNNNNAGG	1 cut(s)	3557
BstFNI	CGCG	2 cut(s)	1327, 3594
BstH2I	RGCGCY	1 cut(s)	2764
BstHHI	GCGC	2 cut(s)	564, 2763
BstMAI	GTCTC	4 cut(s)	214, 2189, 3086, 3158
BstMWI	GCNNNNNNNGC	8 cut(s)	1628, 2048, 2243, 2507, 3302, 3545, 3692, 3701
BstNI	CCWGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
BstNSI	RCATGY	5 cut(s)	202, 330, 1115, 2869, 3533
BstSCI	CCNGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
BstSFI	CTRYAG	4 cut(s)	417, 1986, 2748, 3537
BstSLI	GKGCMC	1 cut(s)	1336
BstSNI	TACGTA	1 cut(s)	944
BstUI	CGCG	2 cut(s)	1327, 3594
BstV2I	GAAGAC	1 cut(s)	281
BstX2I	RGATCY	6 cut(s)	258, 2267, 2349, 3280, 3404, 3574
BstXI	CCANNNNNNTGG	2 cut(s)	1778, 3742
BstYI	RGATCY	6 cut(s)	258, 2267, 2349, 3280, 3404, 3574
BstZ17I	GTATAC	1 cut(s)	2639
BsuI	GTATCC	1 cut(s)	2028
BsuRI	GGCC	8 cut(s)	138, 782, 1324, 1534, 2332, 2576, 2717, 3552
BtgI	CCRYGG	1 cut(s)	1325
BtsI	GCAGTG	4 cut(s)	127, 159, 2498, 2922
BtsIMutI	CAGTG	9 cut(s)	127, 159, 459, 1721, 2060, 2441, 2498, 2817, 2922
BtuMI	TCGCGA	1 cut(s)	3594
BveI	ACCTGC	1 cut(s)	1979
Cac8I	GCNNGC	7 cut(s)	200, 328, 1120, 1242, 1886, 3531, 3550
CaiI	CAGNNNCTG	1 cut(s)	3028
CciI	TCATGA	2 cut(s)	1714, 2956
CfoI	GCGC	2 cut(s)	564, 2763
Cfr10I	RCCGGY	1 cut(s)	1880
Cfr13I	GGNCC	8 cut(s)	182, 548, 781, 1418, 1663, 2575, 3338, 3550
Cfr42I	CCGCGG	1 cut(s)	1328
CseI	GACGC	2 cut(s)	1543, 3175
Csp6I	GTAC	9 cut(s)	694, 1074, 1362, 1401, 1897, 2702, 2825, 3689, 3739
CviQI	GTAC	9 cut(s)	694, 1074, 1362, 1401, 1897, 2702, 2825, 3689, 3739
DdeI	CTNAG	9 cut(s)	462, 1308, 1851, 2025, 2487, 2622, 2858, 3114, 3292
DraI	TTTAAA	3 cut(s)	1515, 2010, 2055
EaeI	YGGCCR	3 cut(s)	1532, 2330, 2715
Eam1104I	CTCTTC	5 cut(s)	942, 1310, 1684, 2112, 3626
EarI	CTCTTC	5 cut(s)	942, 1310, 1684, 2112, 3626
Ecl136II	GAGCTC	1 cut(s)	3654
Eco105I	TACGTA	1 cut(s)	944
Eco130I	CCWWGG	2 cut(s)	658, 2475
Eco147I	AGGCCT	1 cut(s)	138
Eco24I	GRGCYC	1 cut(s)	3656
Eco31I	GGTCTC	2 cut(s)	2189, 3086
Eco32I	GATATC	1 cut(s)	3151
Eco47I	GGWCC	5 cut(s)	182, 548, 1418, 1663, 3338
Eco47III	AGCGCT	1 cut(s)	2762
Eco53kI	GAGCTC	1 cut(s)	3654
Eco57I	CTGAAG	7 cut(s)	435, 1263, 2526, 2841, 3066, 3338, 3608
Eco88I	CYCGRG	2 cut(s)	215, 3750
EcoICRI	GAGCTC	1 cut(s)	3654
EcoNI	CCTNNNNNAGG	1 cut(s)	3557
EcoO109I	RGGNCCY	1 cut(s)	548
EcoRI	GAATTC	2 cut(s)	1695, 3124
EcoRII	CCWGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
EcoRV	GATATC	1 cut(s)	3151
EcoT14I	CCWWGG	2 cut(s)	658, 2475
EcoT38I	GRGCYC	1 cut(s)	3656
ErhI	CCWWGG	2 cut(s)	658, 2475
Esp3I	CGTCTC	1 cut(s)	3158
FalI	AAGNNNNNCTT	8 cut(s)	560, 592, 921, 953, 2233, 2265, 2881, 2913
FaqI	GGGAC	4 cut(s)	592, 1024, 2573, 3249
FauI	CCCGC	2 cut(s)	1080, 3541
FbaI	TGATCA	2 cut(s)	637, 688
FblI	GTMKAC	1 cut(s)	2638
FriOI	GRGCYC	1 cut(s)	3656
FspBI	CTAG	9 cut(s)	170, 1133, 1253, 1610, 1788, 1944, 2253, 2282, 3108
GlaI	GCGC	2 cut(s)	563, 2762
GsaI	CCCAGC	1 cut(s)	2097
GsuI	CTGGAG	5 cut(s)	468, 1071, 1800, 2867, 3332
HaeII	RGCGCY	1 cut(s)	2764
HaeIII	GGCC	8 cut(s)	138, 782, 1324, 1534, 2332, 2576, 2717, 3552
HapII	CCGG	1 cut(s)	1881
HgaI	GACGC	2 cut(s)	1543, 3175
HhaI	GCGC	2 cut(s)	564, 2763
Hin6I	GCGC	2 cut(s)	562, 2761
HinP1I	GCGC	2 cut(s)	562, 2761
HincII	GTYRAC	3 cut(s)	459, 2539, 3288
HindII	GTYRAC	3 cut(s)	459, 2539, 3288
HindIII	AAGCTT	2 cut(s)	2895, 3600
HpaII	CCGG	1 cut(s)	1881
HphI	GGTGA	5 cut(s)	647, 1584, 2067, 2290, 2311
Hpy166II	GTNNAC	9 cut(s)	459, 1285, 1421, 1493, 2539, 2639, 2817, 3288, 3338
Hpy8I	GTNNAC	9 cut(s)	459, 1285, 1421, 1493, 2539, 2639, 2817, 3288, 3338
HpyAV	CCTTC	7 cut(s)	128, 398, 613, 1287, 1314, 2144, 3553
HpyCH4IV	ACGT	3 cut(s)	943, 2641, 3757
HpyF10VI	GCNNNNNNNGC	8 cut(s)	1628, 2048, 2243, 2507, 3302, 3545, 3692, 3701
HpyF3I	CTNAG	9 cut(s)	462, 1308, 1851, 2025, 2487, 2622, 2858, 3114, 3292
HpySE526I	ACGT	3 cut(s)	943, 2641, 3757
HspAI	GCGC	2 cut(s)	562, 2761
KpnI	GGTACC	1 cut(s)	1077
Ksp22I	TGATCA	2 cut(s)	637, 688
KspI	CCGCGG	1 cut(s)	1328
LmnI	GCTCC	4 cut(s)	629, 1052, 2835, 2851
LweI	GCATC	6 cut(s)	718, 1177, 1814, 2224, 2255, 3050
MaeI	CTAG	9 cut(s)	170, 1133, 1253, 1610, 1788, 1944, 2253, 2282, 3108
MaeII	ACGT	3 cut(s)	943, 2641, 3757
MfeI	CAATTG	1 cut(s)	501
MflI	RGATCY	6 cut(s)	258, 2267, 2349, 3280, 3404, 3574
MhlI	GDGCHC	4 cut(s)	1336, 2927, 3656, 3757
MlsI	TGGCCA	3 cut(s)	1534, 2332, 2717
MluNI	TGGCCA	3 cut(s)	1534, 2332, 2717
MlyI	GAGTC	6 cut(s)	614, 928, 2098, 2621, 2954, 3380
MmeI	TCCRAC	3 cut(s)	289, 363, 1979
Mox20I	TGGCCA	3 cut(s)	1534, 2332, 2717
MroXI	GAANNNNTTC	1 cut(s)	1699
MscI	TGGCCA	3 cut(s)	1534, 2332, 2717
MslI	CAYNNNNRTG	7 cut(s)	44, 452, 717, 1476, 1862, 2532, 2967
Msp20I	TGGCCA	3 cut(s)	1534, 2332, 2717
MspA1I	CMGCKG	1 cut(s)	1327
MspI	CCGG	1 cut(s)	1881
MspR9I	CCNGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
MunI	CAATTG	1 cut(s)	501
Mva1269I	GAATGC	4 cut(s)	1155, 1349, 2527, 2962
MvaI	CCWGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
MvnI	CGCG	2 cut(s)	1327, 3594
MwoI	GCNNNNNNNGC	8 cut(s)	1628, 2048, 2243, 2507, 3302, 3545, 3692, 3701
NlaIV	GGNNCC	4 cut(s)	114, 783, 1075, 1665
NmuCI	GTSAC	6 cut(s)	443, 931, 1590, 1991, 2366, 2439
NruI	TCGCGA	1 cut(s)	3594
NspI	RCATGY	5 cut(s)	202, 330, 1115, 2869, 3533
OliI	CACNNNNGTG	1 cut(s)	452
PaeI	GCATGC	3 cut(s)	202, 330, 3533
PagI	TCATGA	2 cut(s)	1714, 2956
PceI	AGGCCT	1 cut(s)	138
PctI	GAATGC	4 cut(s)	1155, 1349, 2527, 2962
PdmI	GAANNNNTTC	1 cut(s)	1699
PfeI	GAWTC	8 cut(s)	950, 1256, 1435, 1582, 1718, 1874, 2072, 3137
PfoI	TCCNGGA	2 cut(s)	904, 3348
PleI	GAGTC	6 cut(s)	613, 928, 2098, 2620, 2953, 3380
PpsI	GAGTC	6 cut(s)	613, 928, 2098, 2620, 2953, 3380
Ppu21I	YACGTR	1 cut(s)	944
PpuMI	RGGWCCY	1 cut(s)	548
PsiI	TTATAA	1 cut(s)	2082
Psp124BI	GAGCTC	1 cut(s)	3656
Psp5II	RGGWCCY	1 cut(s)	548
Psp6I	CCWGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
PspFI	CCCAGC	1 cut(s)	2093
PspGI	CCWGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
PspN4I	GGNNCC	4 cut(s)	114, 783, 1075, 1665
PspPI	GGNCC	8 cut(s)	182, 548, 781, 1418, 1663, 2575, 3338, 3550
PspPPI	RGGWCCY	1 cut(s)	548
PsrI	GAACNNNNNNTAC	2 cut(s)	400, 432
PstI	CTGCAG	2 cut(s)	1990, 3541
PstNI	CAGNNNCTG	1 cut(s)	3028
PsuI	RGATCY	6 cut(s)	258, 2267, 2349, 3280, 3404, 3574
RruI	TCGCGA	1 cut(s)	3594
RsaI	GTAC	9 cut(s)	695, 1075, 1363, 1402, 1898, 2703, 2826, 3690, 3740
RsaNI	GTAC	9 cut(s)	694, 1074, 1362, 1401, 1897, 2702, 2825, 3689, 3739
RseI	CAYNNNNRTG	7 cut(s)	44, 452, 717, 1476, 1862, 2532, 2967
SacI	GAGCTC	1 cut(s)	3656
SacII	CCGCGG	1 cut(s)	1328
Sau96I	GGNCC	8 cut(s)	182, 548, 781, 1418, 1663, 2575, 3338, 3550
ScaI	AGTACT	2 cut(s)	1363, 2703
SchI	GAGTC	6 cut(s)	614, 928, 2098, 2621, 2954, 3380
ScrFI	CCNGG	7 cut(s)	110, 291, 668, 906, 2885, 3350, 3736
SduI	GDGCHC	4 cut(s)	1336, 2927, 3656, 3757
SfaNI	GCATC	6 cut(s)	718, 1177, 1814, 2224, 2255, 3050
SfcI	CTRYAG	4 cut(s)	417, 1986, 2748, 3537
Sfr303I	CCGCGG	1 cut(s)	1328
SgrBI	CCGCGG	1 cut(s)	1328
SinI	GGWCC	5 cut(s)	182, 548, 1418, 1663, 3338
SmiMI	CAYNNNNRTG	7 cut(s)	44, 452, 717, 1476, 1862, 2532, 2967
SmlI	CTYRAG	3 cut(s)	1000, 1646, 3655
SmoI	CTYRAG	3 cut(s)	1000, 1646, 3655
SnaBI	TACGTA	1 cut(s)	944
SpeI	ACTAGT	1 cut(s)	3107
SphI	GCATGC	3 cut(s)	202, 330, 3533
SseBI	AGGCCT	1 cut(s)	138
SspI	AATATT	5 cut(s)	22, 283, 2169, 2523, 3061
SspMI	CTAG	9 cut(s)	170, 1133, 1253, 1610, 1788, 1944, 2253, 2282, 3108
SstI	GAGCTC	1 cut(s)	3656
StuI	AGGCCT	1 cut(s)	138
StyD4I	CCNGG	7 cut(s)	108, 289, 666, 904, 2883, 3348, 3734
StyI	CCWWGG	2 cut(s)	658, 2475
TaiI	ACGT	3 cut(s)	946, 2644, 3760
TaqI	TCGA	4 cut(s)	649, 715, 807, 872
TaqII	GACCGA	1 cut(s)	1406
TatI	WGTACW	5 cut(s)	693, 1361, 2701, 2824, 3688
TauI	GCSGC	3 cut(s)	782, 1327, 2407
TfiI	GAWTC	8 cut(s)	950, 1256, 1435, 1582, 1718, 1874, 2072, 3137
TscAI	CASTG	9 cut(s)	127, 159, 459, 1728, 2067, 2448, 2498, 2824, 2922
TseFI	GTSAC	6 cut(s)	443, 931, 1590, 1991, 2366, 2439
Tsp45I	GTSAC	6 cut(s)	443, 931, 1590, 1991, 2366, 2439
TspGWI	ACGGA	2 cut(s)	1594, 3697
TspRI	CASTG	9 cut(s)	127, 159, 459, 1728, 2067, 2448, 2498, 2824, 2922
VpaK11BI	GGWCC	5 cut(s)	182, 548, 1418, 1663, 3338
XagI	CCTNNNNNAGG	1 cut(s)	3557
XbaI	TCTAGA	2 cut(s)	1787, 2252
XceI	RCATGY	5 cut(s)	202, 330, 1115, 2869, 3533
XmiI	GTMKAC	1 cut(s)	2638
XmnI	GAANNNNTTC	1 cut(s)	1699
XspI	CTAG	9 cut(s)	170, 1133, 1253, 1610, 1788, 1944, 2253, 2282, 3108
ZrmI	AGTACT	2 cut(s)	1363, 2703

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.