Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Forward (+)

81507042 .. 81522662

Gene Structure 15621 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG514100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5445 bp

ATGCATCTTTACAATGCTTGGCTTCCTCCACCTGTCGCCGAGGAGTCCATCAAGGAGAGGGACTCCTTCTCTCGCGTCGTCTCCTCCGTCAAGAACTCCTACCGCTCCGATGACCCTGATTCAGTCTATGCAACTCTCAAATGGGTCTCTGTCATTGACCTTTTCATCAAGGCAAAGAGTGAAGTGTCTATGGAAGATGCGAGTGGACTGATTGAGTTTGGATTGGAGTTGTTTCATGTATCGGAGAATAAGCTTTATGCACAGGTTAGGTGGGGAAATATTCTTGTGAAATTACTCAACAAGTATCGGAAGAAGTTGTCGTTGAAGGTAAAGTGGCGCCCTTTGTATGATACTCTGATTCATACACATTTCAGTAGGAATACAGGTCCTGAAGGCTGGAGATTGAGACAACGGCATTTTGAGGCTACTACTTCTCTTGTTCGGTCTTGCCGGAAGTTCTTTCCCCCGGGCTCTGCCGTTGAGATATGGTCTGAGTTTAGATCTCTGTTGGAAAATCCTTGGCATAACTCATCCTTTGAGGGATCTGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGAATTCTTTACACATGATTGGATCAAGGAGTTAATGGACCTGTGGGACTCGATACCAAATTGCCAATTTTGGAATAGTCAATGGACAGCTATTATAGCTCGTGTTGTAAAGAACTACAGATTTATTGATTGGGAGAGTTACTTGCCTACACTTTTCACTAGATTCTTAAATATGTTTGAGGTTCCTGTTGCAAATGGAAGTGGATCGTATCCCTTTTCTGTGGATGTTCCGAGAAACACAAGGTTCTTGTTCTCCAATAGGACAGTTACCCCAGCAAAGGGCATTGCAAAGTCAATAGTCTATCTATTGAAACCTGGTAGCTCGGTGCTGGAGCATTTTGAAAAATTGGTCAACCTTTTAGAACAATATTACCATCCTTCTAACGGCGGCCGCTGGACTTACTCATTAGAGCGTTTTTTGCTCCATTTAGTAATTTCATTCCAGAAGCGCTTACAGAATGAACAATTGAAAGTAAATAAAGATAGTCAGGATGAACAGTATCTAGGAAAATCAGAAAGACATTTCTTTGTTAAAGTGGTGCTGAAGTTAATTGATCGCGGTCAATATAGCAAGAATGAACATCTTTCTGAGACAGTAGCTGCTGCAACTTCAATTTTATCTTACGTGGAGCCTTCTCTAATCCTTCCTTTTGTTGCATCTCGCTTCCATATGGCATTGGAGACGATGACTGCCACCCACCAGTTGCAAGTTGCTGTGATGTCAGTAGCATTTGTTGGCCGTTCATTATTCCTCAAATCCTTGTCAACCTCTACAGTAAAATCAATTGATATTGGTAGTGGTGAAGCTGGTGATGAGTTCATTGAGCTACTGATGGTTTCGTTATCGAATGCATTACTTGGTATGGATGCCAATGATCCTCCTAAAACCTTGGCAACAATGCAATTAATTGGTTCTATATTTTCCAATATGTCTTCATTGGATGATGAGGTGTCAGTCATGCCAATGATTCGCTTTTCTGAATGGTTGGATGAGTTCTTCTGTCGCTTATTTGCCTTACTTCTACACTTGGAACCAAGCAGTGTTACGAATGAAGGCTTGCACTCATCAGCAACATCAGGAACTTTTCTGGTTGAGGATGGTCCTTATTACTATTGCATGCTTGAAATCTTGTTTGGAAGACTCTCAAAACCGCTCTATAATCAGGCTTTGAAGAAGATATCCAAGTTTGTCAAGACAAATATCCTTCCTGGGGCAATTGCAGAGGTCGGACTGCTTTGTTGTGCATGTGTTTATTCAAACCCAGAAGAAGCAGTTACTCAACTGGTTGAGCCAATTTTACTGTCTGTTATATCCTCTTTAGAAGGAACACCGTCTACAGGATTTGGAGGTAGAGGAATATGTGATGCTTCAGTTTCAACCAAGGCTAAACCGACAATTTCTCCGGCTCTTGAAACAGCAATTGATTATCAATTAAAAATATTATCAGTTGCCATAAGTTATGGAGGTCCTGCTCTTCTGCGATACAAGGATCAGTTCAAAGAGGCTGTTGTTTCTGCTTTTGAATCTCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGGTCTCTCCTTGGAAGCTTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTATTTTGCATCACCCAAATGCTACTGCATTAGAGGAATGGATCAGCTCAAAAGATTATTCAGACGATAAACCATTGATGGGCCCTAAATGGCATATCCCTAGTGTTGAGGAAGTTAAATTTGCAAATGAACTTTTGGACCTTCATTTCCGGTCGGCTTTGGATGATCTTCTAAGAATATGTCAAACTAAAGTACATTCTGATCCAGGAGATGAGAAGGAGCACTTAAAAGTGACTCTTTTGCGAATAGATTCTTCATTGCAAGGTGTTTTGACTTGCTTGCCCGACTTCACCCCATCATCAAGGAATGGAACTGTCGAAAATCCAAATCATGGTTCCTTTTTAATAGCAGGAGCAACAGGTGCAACTGTGGGCAACACTAAATTGCGGGAAAAAGCTGCTGAGATTATACATGCAGCCTGCAAGTATTTACTTGAGAACAAATCAGATGACAGCATCCTCTTGGTCCTCATCATTCGTATTATGGATGCTTTAGGAAATTATGGAAGTATGGAGTATGATGAATGGGCCAATCACAGGCAGGCATGGAAATTGGAATCTGCTGCCATTATAGAGCCTTCAATAAATTTTATTGTTTCAGCTCACTCTAAAGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCCTTCATGCACAATACTTGGAGATCATCCCAGTCATCCTACCATATATTTCGCACTGGGAATTTTGGTCCACCAGAACATGTGAATCTTTTGGTGGATGATCTTCTAAATCTCTCTTTGCATAGCTATGAAACTGTTCGAATACTTGCTGGGAAATATCTTTTAAAAATGATAAAGAGATGGCCATCTATGATTTCAAAGTGTGTTCTCTCTCACACTGAGAACTTACGAAACCCCAAGGCACCAGAATATGCAGTGCTTGGTTCTTGTGCAGTCCTTGCTACACAAACAGTTCTCAAGCATTTGACAATGGACCCTAAGTCATTCTCTTCATTTATCCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAAACCCAGAAAGCTATCAACGAGCTTTTTGTCAAATACAACATTCACTTTGCTGGAGTATCGAGAAGCTATTTCAATTCATCAGACAATCATGTTGATGCACCAGATTTTGCAGATTTGGTTTCTCAGATCAGTTCTATGAGTTTTGATTCTATGGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTTTATTGCTGTTGGCTATGGCATCTAGGAATGACCCAAGTTCCTCCTCCAAAATTCTGAGTGAAACTGCTGGTCACTTCCTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTAGCAATCTCAGCTCTAAATACGCTATTAAAAGAATCACCTTATAAACTGTCAGATGAAAAACAGAGTTTTTCCTCAGAGAAATTGCAAGGAGCTAGAAAATCGTCTCTTGAAGGAGAGTTAACTCAAATATTTCAGGAAGATGGATTTTTCAGTGAGACTTTGAATAGTCTTTCTCATGTTCATATAATATCTGATACTGAAAGCAGTACTAGAAGCAATCATGGGAACTCTTCTTTTCAGAGCCTAGCAGACAAATCAATCACCCGCTTTTATTTTGACTTTACCTCTTCATGGCCACGCACTCCAACTTGGATCTCTTTATTAGGAAGTGACACCTTTTACTCAAACTATGCGAGAATATTCAAACGGCTAATACAAGAATGTGGTATGCCCGTCGTAGTGGCACTGAAAAGTTCATTGGAGGAGTTCTCAAATGCCAAGGAGAGGTCCAAGCAGTGCGTTGCTGCTGAAGCTTTCGCTGGAATGTTGCATTCTGATGTTAATGGTATCAAAGAAGCATGGGATAATTGGATGGTGGTCCAGTTGCAGAACATAATTCTAGCTCATTCAGTAGAATCAATACCCGAATGGACAGCCTGCATACGTTATGCAGTTACCGGGAAAGGGAAATATGGAACTAGAGTTCCTCTTTTGAGACAACCAGTACTAGATTGTCTGGCTACACCTTTACCAGGAACAGTCACTACCACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCAGCCGCCCTTGTAGAACTATCTCCCCAGAAAATGCCATTGGCTGAAATTCAGCTCCACTATAGACTTCTGGAGGAGTTGCTCGGTAACATGTGCCATTCATCTGCCCAAGTAAGAGAAGCTATAGGTGTTGCCCTTTCTGTGTTGTGCTCTAACATTCGTCTTTATGAGTCATTTGATCATGACGGTTCAAATGGGAGTGCTAGTAAAGGTCTTGGCAACCAATTTGATGGGAGAAGTTGGGTTCTGTTTTTAAAAGAGCGAGCGTCGGAAGTGGTAATGAATATTCAGAATAGCACTCATTCTGACAGTATGGAAACCCCACAAAATATTACTCCTGAAAACGGACATTTGAATGGGGATTCACAAGCTGATGTCAAATGGATGGAAACGCTTTTCCATTTTATCATCTCATCGTTGAGGTCTGCGAGATCATCATATCTGGTGGATGTAATTGTGGGATTTCTTTATCCTGTGATATCATTGCAGGAAACTTCAAGTAAAGAACTGTCAACCTTGGCCAAGGCAGCTTTTGAGTTGCTAAAATGGAGGATTTTTTGGGGACCTCATCTCCAAGAGGCCGTATCTGTGATTCTTTCTTCTGCCAATGACTCTAACTGGCGCACTAGATCTGCAACATTGACATATCTGCGGACTTTTATGTACAGGCACACTTTCATTCTCTCAATTGGGGAGAAACAACAAATTTGGAGAACTGTTGAAAAGTTACTTGTAGACAACCAAGTTGAGGTAAGAGAACATGCTGCAGCTGTTTTGGCGGGCCTAACAAAGGGTGGCGATGAAGATCTAGCTAAAGACTTTCGTGACAAAGCTTACAAAGAAGCTACTGATCTTCAGAGGAAGAGAAAACGGAGAAATTTGAGTTCAAGCGAACCCATAGCTTCCATTCATGGTGCTGTACTTGCTTTAGTTGCTTCCGTTCTGTCAGCGCCTTATGATATGCCCAGTTGGTTGCCAGATCATGTCACTTTACTTGCTCGTTTTGCTGGAGAACCAGCACCAGTGAAATCTACGGTTACAAAAGCAGTTGCAGAATTTCGACGAACCCATGCTGATACATGGAACATTCAGAAAGACTCATTTACCGAAGAGCAACTCGAGGTTCTGGCTGATACTTCTTCTTCATCTTCATATTTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1814

Amino Acids

203.83

Weight (kDa)

6.89

Isoelectric Point (pI)

43.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	310 - 506	5.1e-29	Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid	PF16507	517 - 825	1.7e-19	Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C	PF11919	1729 - 1814	6.1e-28	Proteasome activator complex subunit 4-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3585
AccB1I	GGYRCC	2 cut(s)	336, 3094
AccB7I	CCANNNNNTGG	1 cut(s)	2537
AccBSI	CCGCTC	2 cut(s)	105, 1741
AccI	GTMKAC	2 cut(s)	1922, 5016
AccII	CGCG	3 cut(s)	75, 1146, 2833
AclWI	GGATC	8 cut(s)	550, 617, 799, 1457, 2085, 2255, 2402, 3863
AcoI	YGGCCR	5 cut(s)	976, 1324, 3035, 3836, 4800
AcuI	CTGAAG	5 cut(s)	411, 1151, 1941, 4032, 5120
AcyI	GRCGYC	1 cut(s)	337
AfaI	GTAC	7 cut(s)	2201, 2400, 3401, 3751, 4209, 4946, 5202
AfeI	AGCGCT	2 cut(s)	1037, 4268
AflIII	ACRYGT	2 cut(s)	2932, 4371
AhdI	GACNNNNNGTC	1 cut(s)	3172
AjnI	CCWGG	5 cut(s)	585, 901, 1795, 2410, 4234
AjuI	GAANNNNNNNTTGG	8 cut(s)	1704, 1736, 2524, 2556, 3944, 3976, 4304, 4336
AloI	GAACNNNNNNTCC	6 cut(s)	2160, 2192, 4170, 4171, 4202, 4203
Alw21I	GWGCWC	2 cut(s)	2430, 4433
Alw26I	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
AlwI	GGATC	8 cut(s)	550, 617, 799, 1457, 2085, 2255, 2402, 3863
AlwNI	CAGNNNCTG	2 cut(s)	389, 1187
Ama87I	CYCGRG	2 cut(s)	466, 5399
Aor51HI	AGCGCT	2 cut(s)	1037, 4268
ApaI	GGGCCC	1 cut(s)	2291
ApeKI	GCWGC	9 cut(s)	1187, 1190, 2603, 2621, 2768, 4007, 4808, 5045, 5048
AseI	ATTAAT	1 cut(s)	1493
Asp700I	GAANNNNTTC	3 cut(s)	2455, 2988, 3202
AspLEI	GCGC	5 cut(s)	339, 1038, 4269, 4905, 5233
AsuC2I	CCSGG	3 cut(s)	467, 468, 4162
AsuHPI	GGTGA	7 cut(s)	1400, 1409, 2153, 2210, 2488, 3570, 3796
AsuII	TTCGAA	1 cut(s)	2992
AvaI	CYCGRG	2 cut(s)	466, 5399
BaeGI	GKGCMC	2 cut(s)	2291, 2842
BalI	TGGCCA	3 cut(s)	3037, 3838, 4802
BanI	GGYRCC	2 cut(s)	336, 3094
BanII	GRGCYC	2 cut(s)	473, 2291
BauI	CACGAG	1 cut(s)	687
BbsI	GAAGAC	2 cut(s)	1512, 1732
Bbv12I	GWGCWC	2 cut(s)	2430, 4433
BbvI	GCAGC	9 cut(s)	1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BceAI	ACGGC	6 cut(s)	428, 461, 988, 1311, 3926, 4847
BciT130I	CCWGG	5 cut(s)	587, 903, 1797, 2412, 4236
BciVI	GTATCC	2 cut(s)	807, 3531
BclI	TGATCA	3 cut(s)	2143, 2194, 4459
BcnI	CCSGG	3 cut(s)	467, 468, 4162
BcoDI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BfmI	CTRYAG	7 cut(s)	703, 1359, 1923, 4254, 4342, 4404, 5046
BfoI	RGCGCY	4 cut(s)	340, 1039, 4270, 5234
BfuI	GTATCC	2 cut(s)	807, 3531
BglII	AGATCT	3 cut(s)	500, 4910, 5086
BmcAI	AGTACT	2 cut(s)	3751, 4209
Bme1390I	CCNGG	8 cut(s)	467, 468, 587, 903, 1797, 2412, 4162, 4236
BmeRI	GACNNNNNGTC	1 cut(s)	3172
BmeT110I	CYCGRG	2 cut(s)	466, 5399
BmiI	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
BmrFI	CCNGG	8 cut(s)	467, 468, 587, 903, 1797, 2412, 4162, 4236
BmrI	ACTGGG	3 cut(s)	2878, 2919, 5241
BmuI	ACTGGG	3 cut(s)	2878, 2919, 5241
BpiI	GAAGAC	2 cut(s)	1512, 1732
BplI	GAGNNNNNCTC	4 cut(s)	47, 79, 3956, 3988
BpmI	CTGGAG	5 cut(s)	418, 938, 3303, 4373, 5310
Bpu14I	TTCGAA	1 cut(s)	2992
BpuEI	CTTGAG	2 cut(s)	2660, 3134
BpuMI	CCSGG	3 cut(s)	467, 468, 4162
BsaAI	YACGTR	1 cut(s)	1213
BsaBI	GATNNNNATC	2 cut(s)	3037, 5085
BsaHI	GRCGYC	1 cut(s)	337
BsaI	GGTCTC	2 cut(s)	151, 2163
BsaWI	WCCGGW	1 cut(s)	2355
BsaXI	ACNNNNNCTCC	6 cut(s)	1972, 2002, 4471, 4501, 4836, 4866
Bse1I	ACTGG	9 cut(s)	1288, 1875, 2884, 2914, 4084, 4205, 4904, 5247, 5303
Bse3DI	GCAATG	3 cut(s)	870, 2462, 4763
Bse8I	GATNNNNATC	2 cut(s)	3037, 5085
BseBI	CCWGG	5 cut(s)	587, 903, 1797, 2412, 4236
BseJI	GATNNNNATC	2 cut(s)	3037, 5085
BseMI	GCAATG	3 cut(s)	870, 2462, 4763
BseMII	CTCAG	9 cut(s)	483, 1167, 2598, 3063, 3368, 3470, 3542, 3564, 3630
BseNI	ACTGG	9 cut(s)	1288, 1875, 2884, 2914, 4084, 4205, 4904, 5247, 5303
BseRI	GAGGAG	5 cut(s)	56, 73, 3457, 3980, 4370
BseSI	GKGCMC	2 cut(s)	2291, 2842
BseX3I	CGGCCG	1 cut(s)	976
BseXI	GCAGC	9 cut(s)	1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BseYI	CCCAGC	2 cut(s)	859, 3002
BsgI	GTGCAG	1 cut(s)	3144
Bsh1236I	CGCG	3 cut(s)	75, 1146, 2833
Bsh1285I	CGRYCG	2 cut(s)	979, 2360
BshNI	GGYRCC	2 cut(s)	336, 3094
BsiEI	CGRYCG	2 cut(s)	979, 2360
BsiHKAI	GWGCWC	2 cut(s)	2430, 4433
BsiHKCI	CYCGRG	2 cut(s)	466, 5399
BsiSI	CCGG	5 cut(s)	451, 467, 1991, 2356, 4161
BslFI	GGGAC	3 cut(s)	74, 647, 4857
BsmAI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BsmBI	CGTCTC	3 cut(s)	85, 1262, 3651
BsmFI	GGGAC	3 cut(s)	74, 647, 4857
BsmI	GAATGC	2 cut(s)	1441, 4033
Bso31I	GGTCTC	2 cut(s)	151, 2163
BsoBI	CYCGRG	2 cut(s)	466, 5399
Bsp119I	TTCGAA	1 cut(s)	2992
Bsp120I	GGGCCC	1 cut(s)	2287
Bsp1286I	GDGCHC	5 cut(s)	473, 2291, 2430, 2842, 4433
Bsp1407I	TGTACA	1 cut(s)	4944
BspCNI	CTCAG	9 cut(s)	484, 1168, 2599, 3064, 3367, 3471, 3541, 3563, 3629
BspFNI	CGCG	3 cut(s)	75, 1146, 2833
BspHI	TCATGA	2 cut(s)	3217, 4462
BspLI	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
BspMAI	CTGCAG	1 cut(s)	5050
BspPI	GGATC	8 cut(s)	550, 617, 799, 1457, 2085, 2255, 2402, 3863
BspQI	GCTCTTC	2 cut(s)	2067, 5385
BspT104I	TTCGAA	1 cut(s)	2992
BspT107I	GGYRCC	2 cut(s)	336, 3094
BspTNI	GGTCTC	2 cut(s)	151, 2163
BsrBI	CCGCTC	2 cut(s)	105, 1741
BsrDI	GCAATG	3 cut(s)	870, 2462, 4763
BsrGI	TGTACA	1 cut(s)	4944
BsrI	ACTGG	9 cut(s)	1288, 1875, 2884, 2914, 4084, 4205, 4904, 5247, 5303
BssNI	GRCGYC	1 cut(s)	337
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	9 cut(s)	518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Bst2BI	CACGAG	1 cut(s)	687
Bst2UI	CCWGG	5 cut(s)	587, 903, 1797, 2412, 4236
Bst6I	CTCTTC	7 cut(s)	2067, 2816, 3187, 3778, 3835, 5138, 5385
BstACI	GRCGYC	1 cut(s)	337
BstAPI	GCANNNNNTGC	2 cut(s)	2567, 3131
BstAUI	TGTACA	1 cut(s)	4944
BstBAI	YACGTR	1 cut(s)	1213
BstBI	TTCGAA	1 cut(s)	2992
BstC8I	GCNNGC	9 cut(s)	1646, 1706, 2486, 2626, 2748, 3389, 4141, 4545, 5062
BstDSI	CCRYGG	1 cut(s)	2831
BstENI	CCTNNNNNAGG	2 cut(s)	4233, 5069
BstFNI	CGCG	3 cut(s)	75, 1146, 2833
BstH2I	RGCGCY	4 cut(s)	340, 1039, 4270, 5234
BstHHI	GCGC	5 cut(s)	339, 1038, 4269, 4905, 5233
BstMAI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2163, 3651, 3692, 4192
BstMCI	CGRYCG	2 cut(s)	979, 2360
BstNI	CCWGG	5 cut(s)	587, 903, 1797, 2412, 4236
BstNSI	RCATGY	6 cut(s)	1708, 1836, 2621, 2936, 4375, 5045
BstSCI	CCNGG	8 cut(s)	465, 466, 585, 901, 1795, 2410, 4160, 4234
BstSFI	CTRYAG	7 cut(s)	703, 1359, 1923, 4254, 4342, 4404, 5046
BstSLI	GKGCMC	2 cut(s)	2291, 2842
BstUI	CGCG	3 cut(s)	75, 1146, 2833
BstV1I	GCAGC	9 cut(s)	1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
BstV2I	GAAGAC	2 cut(s)	1512, 1732
BstX2I	RGATCY	5 cut(s)	500, 542, 3855, 4910, 5086
BstXI	CCANNNNNNTGG	1 cut(s)	4511
BstYI	RGATCY	5 cut(s)	500, 542, 3855, 4910, 5086
BstZI	CGGCCG	1 cut(s)	976
BsuI	GTATCC	2 cut(s)	807, 3531
BtgI	CCRYGG	1 cut(s)	2831
BtgZI	GCGATG	1 cut(s)	5094
BtsI	GCAGTG	3 cut(s)	1633, 3114, 4004
Cac8I	GCNNGC	9 cut(s)	1646, 1706, 2486, 2626, 2748, 3389, 4141, 4545, 5062
CaiI	CAGNNNCTG	2 cut(s)	389, 1187
CciI	TCATGA	2 cut(s)	3217, 4462
CciNI	GCGGCCGC	1 cut(s)	976
CfoI	GCGC	5 cut(s)	339, 1038, 4269, 4905, 5233
Cfr42I	CCGCGG	1 cut(s)	2834
Cfr9I	CCCGGG	1 cut(s)	466
CseI	GACGC	2 cut(s)	64, 4536
CsiI	ACCWGGT	1 cut(s)	901
Csp6I	GTAC	7 cut(s)	2200, 2399, 3400, 3750, 4208, 4945, 5201
CspCI	CAANNNNNGTGG	2 cut(s)	4240, 4275
CviQI	GTAC	7 cut(s)	2200, 2399, 3400, 3750, 4208, 4945, 5201
DinI	GGCGCC	1 cut(s)	338
DraI	TTTAAA	3 cut(s)	3018, 3513, 4536
DriI	GACNNNNNGTC	1 cut(s)	3172
EaeI	YGGCCR	5 cut(s)	976, 1324, 3035, 3836, 4800
EagI	CGGCCG	1 cut(s)	976
Eam1104I	CTCTTC	7 cut(s)	2067, 2816, 3187, 3778, 3835, 5138, 5385
Eam1105I	GACNNNNNGTC	1 cut(s)	3172
EarI	CTCTTC	7 cut(s)	2067, 2816, 3187, 3778, 3835, 5138, 5385
EclXI	CGGCCG	1 cut(s)	976
Eco130I	CCWWGG	9 cut(s)	518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Eco147I	AGGCCT	1 cut(s)	2855
Eco24I	GRGCYC	2 cut(s)	473, 2291
Eco31I	GGTCTC	2 cut(s)	151, 2163
Eco32I	GATATC	2 cut(s)	1767, 4761
Eco47III	AGCGCT	2 cut(s)	1037, 4268
Eco52I	CGGCCG	1 cut(s)	976
Eco57I	CTGAAG	5 cut(s)	411, 1151, 1941, 4032, 5120
Eco88I	CYCGRG	2 cut(s)	466, 5399
EcoNI	CCTNNNNNAGG	2 cut(s)	4233, 5069
EcoO109I	RGGNCCY	4 cut(s)	386, 2054, 2288, 4844
EcoRI	GAATTC	2 cut(s)	590, 3198
EcoRII	CCWGG	5 cut(s)	585, 901, 1795, 2410, 4234
EcoRV	GATATC	2 cut(s)	1767, 4761
EcoT14I	CCWWGG	9 cut(s)	518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
EcoT22I	ATGCAT	2 cut(s)	6, 1441
EcoT38I	GRGCYC	2 cut(s)	473, 2291
EgeI	GGCGCC	1 cut(s)	338
EheI	GGCGCC	1 cut(s)	338
ErhI	CCWWGG	9 cut(s)	518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
Esp3I	CGTCTC	3 cut(s)	85, 1262, 3651
FalI	AAGNNNNNCTT	4 cut(s)	2414, 2446, 2427, 2459
FaqI	GGGAC	3 cut(s)	74, 647, 4857
FauI	CCCGC	3 cut(s)	2586, 3815, 5053
FauNDI	CATATG	1 cut(s)	1257
FbaI	TGATCA	3 cut(s)	2143, 2194, 4459
FblI	GTMKAC	2 cut(s)	1922, 5016
FriOI	GRGCYC	2 cut(s)	473, 2291
GlaI	GCGC	5 cut(s)	338, 1037, 4268, 4904, 5232
GsaI	CCCAGC	2 cut(s)	863, 3006
GsuI	CTGGAG	5 cut(s)	418, 938, 3303, 4373, 5310
HaeII	RGCGCY	4 cut(s)	340, 1039, 4270, 5234
HapII	CCGG	5 cut(s)	451, 467, 1991, 2356, 4161
HgaI	GACGC	2 cut(s)	64, 4536
HhaI	GCGC	5 cut(s)	339, 1038, 4269, 4905, 5233
Hin1I	GRCGYC	1 cut(s)	337
Hin6I	GCGC	5 cut(s)	337, 1036, 4267, 4903, 5231
HinP1I	GCGC	5 cut(s)	337, 1036, 4267, 4903, 5231
HincII	GTYRAC	4 cut(s)	940, 1353, 3663, 4794
HindII	GTYRAC	4 cut(s)	940, 1353, 3663, 4794
HindIII	AAGCTT	4 cut(s)	251, 2170, 4014, 5112
HpaI	GTTAAC	1 cut(s)	3663
HpaII	CCGG	5 cut(s)	451, 467, 1991, 2356, 4161
HphI	GGTGA	7 cut(s)	1400, 1409, 2153, 2210, 2488, 3570, 3796
Hpy166II	GTNNAC	8 cut(s)	206, 940, 1353, 1923, 2924, 3663, 4794, 5017
Hpy8I	GTNNAC	8 cut(s)	206, 940, 1353, 1923, 2924, 3663, 4794, 5017
Hpy99I	CGWCG	4 cut(s)	80, 3941, 4552, 5346
HpyCH4IV	ACGT	2 cut(s)	1212, 4147
HpySE526I	ACGT	2 cut(s)	1212, 4147
Hsp92I	GRCGYC	1 cut(s)	337
HspAI	GCGC	5 cut(s)	337, 1036, 4267, 4903, 5231
KasI	GGCGCC	1 cut(s)	336
Ksp22I	TGATCA	3 cut(s)	2143, 2194, 4459
KspAI	GTTAAC	1 cut(s)	3663
KspI	CCGCGG	1 cut(s)	2834
LguI	GCTCTTC	2 cut(s)	2067, 5385
LmnI	GCTCC	9 cut(s)	110, 919, 1014, 1216, 2135, 2425, 2558, 3632, 4341
Lsp1109I	GCAGC	9 cut(s)	1174, 1177, 2590, 2633, 2755, 3994, 4820, 5032, 5060
MabI	ACCWGGT	1 cut(s)	901
MaeII	ACGT	2 cut(s)	1212, 4147
MbiI	CCGCTC	2 cut(s)	105, 1741
MfeI	CAATTG	5 cut(s)	1052, 1371, 1803, 2007, 4968
MflI	RGATCY	5 cut(s)	500, 542, 3855, 4910, 5086
MhlI	GDGCHC	5 cut(s)	473, 2291, 2430, 2842, 4433
MlsI	TGGCCA	3 cut(s)	3037, 3838, 4802
MluNI	TGGCCA	3 cut(s)	3037, 3838, 4802
Mly113I	GGCGCC	1 cut(s)	337
MlyI	GAGTC	8 cut(s)	53, 56, 629, 1722, 2434, 4460, 4886, 5372
MmeI	TCCRAC	5 cut(s)	489, 1554, 1795, 3872, 4530
Mox20I	TGGCCA	3 cut(s)	3037, 3838, 4802
Mph1103I	ATGCAT	2 cut(s)	6, 1441
MroXI	GAANNNNTTC	3 cut(s)	2455, 2988, 3202
MscI	TGGCCA	3 cut(s)	3037, 3838, 4802
MslI	CAYNNNNRTG	6 cut(s)	1550, 2223, 2979, 3324, 4038, 4635
Msp20I	TGGCCA	3 cut(s)	3037, 3838, 4802
MspA1I	CMGCKG	3 cut(s)	981, 2833, 5051
MspI	CCGG	5 cut(s)	451, 467, 1991, 2356, 4161
MspR9I	CCNGG	8 cut(s)	467, 468, 587, 903, 1797, 2412, 4162, 4236
MunI	CAATTG	5 cut(s)	1052, 1371, 1803, 2007, 4968
Mva1269I	GAATGC	2 cut(s)	1441, 4033
MvaI	CCWGG	5 cut(s)	587, 903, 1797, 2412, 4236
MvnI	CGCG	3 cut(s)	75, 1146, 2833
NarI	GGCGCC	1 cut(s)	337
NciI	CCSGG	3 cut(s)	467, 468, 4162
NdeI	CATATG	1 cut(s)	1257
NlaIV	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
NmeAIII	GCCGAG	1 cut(s)	64
NmuCI	GTSAC	6 cut(s)	2437, 3494, 3872, 4243, 5105, 5266
NotI	GCGGCCGC	1 cut(s)	976
NsiI	ATGCAT	2 cut(s)	6, 1441
NspI	RCATGY	6 cut(s)	1708, 1836, 2621, 2936, 4375, 5045
NspV	TTCGAA	1 cut(s)	2992
PaeI	GCATGC	1 cut(s)	1708
PaeR7I	CTCGAG	1 cut(s)	5399
PagI	TCATGA	2 cut(s)	3217, 4462
PceI	AGGCCT	1 cut(s)	2855
PciI	ACATGT	2 cut(s)	2932, 4371
PciSI	GCTCTTC	2 cut(s)	2067, 5385
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PctI	GAATGC	2 cut(s)	1441, 4033
PdmI	GAANNNNTTC	3 cut(s)	2455, 2988, 3202
PflMI	CCANNNNNTGG	1 cut(s)	2537
PfoI	TCCNGGA	1 cut(s)	2410
PleI	GAGTC	8 cut(s)	52, 56, 629, 1722, 2434, 4459, 4886, 5372
PluTI	GGCGCC	1 cut(s)	340
PpsI	GAGTC	8 cut(s)	52, 56, 629, 1722, 2434, 4459, 4886, 5372
Ppu21I	YACGTR	1 cut(s)	1213
PpuMI	RGGWCCY	3 cut(s)	386, 2054, 4844
PscI	ACATGT	2 cut(s)	2932, 4371
PshBI	ATTAAT	1 cut(s)	1493
PsiI	TTATAA	1 cut(s)	3585
Psp5II	RGGWCCY	3 cut(s)	386, 2054, 4844
Psp6I	CCWGG	5 cut(s)	585, 901, 1795, 2410, 4234
PspFI	CCCAGC	2 cut(s)	859, 3002
PspGI	CCWGG	5 cut(s)	585, 901, 1795, 2410, 4234
PspN4I	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2289, 2542, 3096, 3168, 4845
PspOMI	GGGCCC	1 cut(s)	2287
PspPPI	RGGWCCY	3 cut(s)	386, 2054, 4844
PspXI	VCTCGAGB	1 cut(s)	5399
PsrI	GAACNNNNNNTAC	4 cut(s)	1906, 1938, 4231, 4263
PstI	CTGCAG	1 cut(s)	5050
PstNI	CAGNNNCTG	2 cut(s)	389, 1187
PsuI	RGATCY	5 cut(s)	500, 542, 3855, 4910, 5086
PvuII	CAGCTG	1 cut(s)	5051
RsaI	GTAC	7 cut(s)	2201, 2400, 3401, 3751, 4209, 4946, 5202
RsaNI	GTAC	7 cut(s)	2200, 2399, 3400, 3750, 4208, 4945, 5201
RseI	CAYNNNNRTG	6 cut(s)	1550, 2223, 2979, 3324, 4038, 4635
SacII	CCGCGG	1 cut(s)	2834
SapI	GCTCTTC	2 cut(s)	2067, 5385
ScaI	AGTACT	2 cut(s)	3751, 4209
SchI	GAGTC	8 cut(s)	53, 56, 629, 1722, 2434, 4460, 4886, 5372
ScrFI	CCNGG	8 cut(s)	467, 468, 587, 903, 1797, 2412, 4162, 4236
SduI	GDGCHC	5 cut(s)	473, 2291, 2430, 2842, 4433
SexAI	ACCWGGT	1 cut(s)	901
SfcI	CTRYAG	7 cut(s)	703, 1359, 1923, 4254, 4342, 4404, 5046
SfoI	GGCGCC	1 cut(s)	338
Sfr274I	CTCGAG	1 cut(s)	5399
Sfr303I	CCGCGG	1 cut(s)	2834
SfuI	TTCGAA	1 cut(s)	2992
SgrBI	CCGCGG	1 cut(s)	2834
SlaI	CTCGAG	1 cut(s)	5399
SmaI	CCCGGG	1 cut(s)	468
SmiMI	CAYNNNNRTG	6 cut(s)	1550, 2223, 2979, 3324, 4038, 4635
SmlI	CTYRAG	3 cut(s)	2639, 3149, 5399
SmoI	CTYRAG	3 cut(s)	2639, 3149, 5399
SphI	GCATGC	1 cut(s)	1708
SseBI	AGGCCT	1 cut(s)	2855
SspDI	GGCGCC	1 cut(s)	336
SspI	AATATT	7 cut(s)	280, 956, 2028, 3672, 3903, 4567, 4612
StuI	AGGCCT	1 cut(s)	2855
StyD4I	CCNGG	8 cut(s)	465, 466, 585, 901, 1795, 2410, 4160, 4234
StyI	CCWWGG	9 cut(s)	518, 1476, 1968, 2164, 3090, 3193, 3981, 4797, 4803
TaiI	ACGT	2 cut(s)	1215, 4150
TaqI	TCGA	7 cut(s)	638, 1433, 2523, 2992, 3290, 5341, 5400
TaqII	GACCGA	2 cut(s)	432, 2145
TatI	WGTACW	6 cut(s)	2199, 2398, 3749, 4207, 4944, 5200
TauI	GCSGC	4 cut(s)	978, 981, 2833, 4289
TseFI	GTSAC	6 cut(s)	2437, 3494, 3872, 4243, 5105, 5266
TseI	GCWGC	9 cut(s)	1187, 1190, 2603, 2621, 2768, 4007, 4808, 5045, 5048
Tsp45I	GTSAC	6 cut(s)	2437, 3494, 3872, 4243, 5105, 5266
TspGWI	ACGGA	4 cut(s)	76, 4641, 5167, 5209
TspMI	CCCGGG	1 cut(s)	466
Van91I	CCANNNNNTGG	1 cut(s)	2537
VspI	ATTAAT	1 cut(s)	1493
XagI	CCTNNNNNAGG	2 cut(s)	4233, 5069
XceI	RCATGY	6 cut(s)	1708, 1836, 2621, 2936, 4375, 5045
XhoI	CTCGAG	1 cut(s)	5399
XmaI	CCCGGG	1 cut(s)	466
XmiI	GTMKAC	2 cut(s)	1922, 5016
XmnI	GAANNNNTTC	3 cut(s)	2455, 2988, 3202
ZrmI	AGTACT	2 cut(s)	3751, 4209
Zsp2I	ATGCAT	2 cut(s)	6, 1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.