Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Forward (+)

79649520 .. 79662691

Gene Structure 13172 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G045680.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5322 bp

ATGCATCTTTACAATGCTTGGCTTCCTCCACCTGTCGCCGAGGAGTCCATCAAGGAGAGGGACTCCTTCTCTCGCGTCGTCTCCTCCGTCAAGAACTCCTACCGCTCCGATGACCCTGATTCAGTCTATGCAACTCTCAAATGGGTCTCTGTCATTGACCTTTTCATCAAGGCAAAGAGTGAAGTGTCTATGGAAGATGCGAGTGGACTGATTGAATTTGGATTGGAGTTGTTTCATGTATCGGAGAATAAGCTTTATGCACAGGTTAGGTGGGGAAATATTCTTGTGAAATTACTCAACAAGTATCGGAAGAAGTTGTCGTTGAAGGTAAAGTGGCGCCCTTTGTATGATACTCTGATTCATACACATTTCAGTAGGAATACAGGTCCTGAAGGCTGGAGATTGAGACAACGGCATTTTGAGGCTACTACTTCTCTTGTTCGGTCTTGCCGGAAGTTCTTTCCCCCAGGCTCTGCCGTTGAGATATGGTCTGAGTTTAGATCTCTGTTGGAAAATCCTTGGCATAACTCATCCTTTGAGGGATCTGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGAATTCTTTACACATGATTGGATCAAGGAGTTAATGGACCTGTGGGACTCGATACCAAATTGCCAATTTTGGAATAGTCAATGGACAGCTATTATAGCTCGTGTTGTAAAGAACTACAGATTTATTGATTGGGAGAGTTACTTGCCTACACTTTTCACTAGATTCTTAAATATGTTTGAGGTTCCTGTTGCAAATGGAAGTGGATCGTATCCCTTTTCTGTGGATGTTCCGAGAAACACAAGGTTCTTGTTCTCCAATAGGACAGTTACCCCAGCAAAGGGCATTGCAAAGTCAATAGTCTATCTATTGAAACCTGGTAGCTCGGTGCTGGAGCACTTTGAAAAATTGGTCAACCTTTTAGAACAATATTACCATCCTTCTAACGGCGGCCGCTGGACTTACTCATTAGAGCGTTTTTTGCTCCATTTAGTAATTTCATTCCAGAAGCGCTTACAGAATGAACAATTGAAAGTAAATAAAGATAGTCAGGATGAACAGTATCTAGGAAAATCAGAAAGGCATTTCTTTGTTAAAGTGGTGCTGAAGTTAATTGATCGCGGTCAATATAGCAAGAATGAACATCTTTCTGAGACAGTAGCTGCTGCAACTTCAATTTTATCTTACGTGGAGCCTTCTCTAATCCTTCCTTTTGTTGCATCTCGCTTCCATATGGCATTGGAGACGATGACTGCCACCCACCAGTTGCAAGTTGCTGTGATGTCAGTAGCATTTGTTGGCCGTTCATTATTCCTCAAATCCTTGTCAACCTCTACAGTAAAATCAATTGATATTGGTAGTGGTGAAGCTGGTGATGAGTTCATTGAGCTACTGATGGTTTCGTTATCGAATGCATTACTTGGTATGGATGCCAATGATCCTCCTAAAACCTTGGCAACAATGCAATTAATTGGTTCTATATTTTCCAATATGTCTTCATTGGATGATGAGGTGTCAGTCATGCCAATGATTCGCTTTTCTGAATGGTTGGATGAGTTCTTCTGTCGCTTATTTGCCTTACTTCTACACTTGGAACCAAGCAGTGCTTTGAAGAAGATATCCAAGTTTGTCAAGACAAATATCCTTCCTGGGGCAATTGCAGAGGTCGGACTGCTTTGTTGTGCATGTGTTTATTCAAACCCAGAAGAAGCAGTTACTCAACTGGTTGAGCCAATTTTACTGTCTGTTATATCCTCTTTAGAAGGAACACCGTCTACAGGATTTGGAGGTAGAGGAATATGTGATGCTTCAGTTTCAACCAAGGCTAAACCGACGATTTCTCCGGCTCTTGAAACAGCAATTGATTATCAATTAAAAATATTATCAGTTGCCATAAGTTATGGAGGTCCTGCTCTTCTGCGATACAAGGATCAGTTCAAAGAGGCTGTTGTTTCTGCTTTTGAATCTCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGGTCTCTCCTTGGAAGCTTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTATTTTGCATCACCCAAATGCTACTGCATTAGAGGAATGGATCAGCTCAAAAGATTATTCAGACGATAAACCATTGATGGGCCCTAAATGGCATATCCCTAGTGTTGAGGAAGTTAAATTTGCAAATGAACTTTTGGACCTTCATTTCCGGTCTGCTTTGGATGATCTTCTAAGAATATGTCAAACTAAAGTACATTCTGATCCAGGAGATGAGAAGGAGCACTTAAAAGTGACTCTTTTGCGAATAGATTCTTCATTGCAAGGTGTTTTGACTTGCTTGCCCGACTTCACCCCATCATCAAGGAATGGAACTGTCGAAAATCCAAATCATGGTTCCTTTTTAATAGCAGGAGCAACAGGTGCAACTGTGGGCAACACTAAATTGCGGGAAAAAGCTGCTGAGATTATACATGCAGCCTGCAAGTATTTACTTGAGAACAAATCAGATGACAGCATCCTCTTGGTCCTCATCATTCGTATTATGGATGCTTTAGGAAATTATGGAAGTATGGAGTATGATGAATGGGCCAATCACAGGCAGGCATGGAAATTGGAATCTGCTGCCATTATAGAGCCTTCAATAAATTTTATTGTTTCAGCTCACTCTAAAGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCCTTCATGCACAATACTTGGAGATCATCCCAGTCATCCTACCATATATTTCGCACTGGGAATTTTGGTCCACCAGAACATGTGAATCTTTTGGTGGATGATCTTCTAAATCTCTCTTTGCATAGCTATGAAACTGTTCGAATACTTGCTGGGAAATATCTTTTAAAAATGATAAAGAGATGGCCATCTATGATTTCAAAGTGTGTTCTCTCTCACACTGAGAACTTACGAAACCCCAAGGCACCAGAATATGCAGTGCTTGGTTCTTGTGCAGTCCTTGCTACACAAACAGTTCTCAAGCATTTGACAATGGACCCTAAGTCATTCTCTTCATTTATCCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAAACCCAGAAAGCTATCAACGAGCTTTTTGTCAAATACAACATTCACTTTGCTGGAGTATCGAGAAGCTATTTCAATTCATCAGACAATCATGTTGATGCACCAGATTTTGCAGATTTGGTTTCTCAGATCAGTTCTATGAGTTTTGATTCTATGGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTTTATTGCTGTTGGCTATGGCATCTAGGAATGACCCAAGTTCCTCCTCCAAAATTCTGAGTGAAACTGCTGGTCACTTCCTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTAGCAATCTCAGCTCTAAATACGCTATTAAAAGAATCACCTTATAAACTGTCAGATGAAAAACAGAGTTTTTCCTCAGAGAAATTGCAAGGAGCTAGAAAATCGTCTCTTGAAGGAGAGTTAACTCAAATATTTCAGGAAGATGGATTTTTCAGTGAGACTTTGAATAGTCTTTCTCATGTTCATATAATATCTGATACTGAAAGCAGTACTAGAGGCAATCATGGGAACTCTTCTTTTCAGAGCCTAGCAGACAAATCAATCACCCGCTTTTATTTTGACTTTACCTCTTCATGGCCACGCACTCCAACTTGGATCTCTTTATTAGGAAGTGACACCTTTTACTCAAACTATGCGAGAATATTCAAACGGCTAATACAAGAATGTGGTATGCCCGTCGTAGTGGCACTGAAAAGTTCATTGGAGGAGTTCTCAAATGCCAAGGAGAGGTCCAAGCAGTGCGTTGCTGCTGAAGCTTTCGCTGGAATGTTGCATTCTGATGTTAATGGTATCAAAGAAGCATGGGATAATTGGATGGTGGTCCAGTTGCAGAACATAATTCTAGCTCATTCAGTAGAATCAATACCCGAATGGACAGCCTGCATACGTTATGCAGTTACCGGGAAAGGGAAATATGGAACTAGAGTTCCTCTTTTGAGACAACCAGTACTAGATTGTCTGGCTACACCTTTACCAGGAACAGTCACTACCACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCAGCCGCCCTTGTAGAACTATCTCCCCAGAAAATGCCATTAGCTGAAATTCAGCTCCACTATAGACTTCTGGAGGAGTTGCTCGGTAACATGTGCCATTCATCTGCCCAAGTAAGAGAAGCTATAGGTGTTGCCCTTTCTGTGTTGTGCTCTAACATTCGTCTTTATGAGTCATTTGATCATGACGGTTCAAATGGGAGTGCTAGTAAAGGTCTTGGCAACCAATTTGATGGGAGAAGTTGGGTTCTGTTTTTAAAAGAGCGAGCGTCGGAAGTGGTAATGAATATTCAGAATAGCACTCATTCTGACAGTATGGAAACCCCACAAAATATTACTCCTGAAAACGGACATTTGAATGGGGATTCACAAGCTGATGTCAAATGGATGGAAACGCTTTTCCATTTTATCATCTCATCGTTGAGGTCTGCGAGATCATCATATCTGGTGGATGTAATTGTGGGATTTCTTTATCCTGTGATATCATTGCAGGAAACTTCAAGTAAAGAACTGTCAACCTTGGCCAAGGCAGCTTTTGAGTTGCTAAAATGGAGGATTTTTTGGGGACCTCATCTCCAAGAGGCCGTATCTGTGATTCTTTCTTCTGCCAATGACTCTAACTGGCGCACTAGATCTGCAACATTGACATATCTGCGGACTTTTATGTACAGGCACACTTTCATTCTCTCAATTGGGGAGAAACAACAAATTTGGAGAACTGTTGAAAAGTTACTTGTAGACAACCAAGTTGAGGTAAGAGAACATGCTGCAGCTGTTTTGGCGGGCCTAACGAAGGGTGGCGATGAAGATCTAGCTAAAGACTTCCGTGACAAAGCTTACAAAGAAGCTACTGATCTTCAGAGGAAGAGAAAACGGAGAAATTTGAGTTCAAGCGAACCCATAGCTTCCATTCATGGTGCTGTACTTGCTTTAGTTGCTTCTGTTCTGTCAGCGCCTTATGATATGCCCAGTTGGTTGCCAGATCATGTCACTTTACTTGCTCGTTTTGCTGGAGAACCAGCACCAGTGAAATCTACGGTTACAAAAGCAGTTGCAGAATTTCGACGAACCCATGCTGATACATGGAACATTCAGAAAGACTCATTTACCGAAGAGCAACTCGAGGTTCTGGCTGATACTTCTTCTTCATCTTCATATTTTGCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1773

Amino Acids

199.2

Weight (kDa)

7.03

Isoelectric Point (pI)

43.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	310 - 506	4.7e-29	Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid	PF16507	543 - 784	5.3e-12	Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C	PF11919	1688 - 1773	5.9e-28	Proteasome activator complex subunit 4-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3462
AccB1I	GGYRCC	2 cut(s)	336, 2971
AccB7I	CCANNNNNTGG	1 cut(s)	2414
AccBSI	CCGCTC	1 cut(s)	105
AccI	GTMKAC	2 cut(s)	1799, 4893
AccII	CGCG	3 cut(s)	75, 1146, 2710
AclWI	GGATC	8 cut(s)	550, 617, 799, 1457, 1962, 2132, 2279, 3740
AcoI	YGGCCR	5 cut(s)	976, 1324, 2912, 3713, 4677
AcuI	CTGAAG	5 cut(s)	411, 1151, 1818, 3909, 4997
AcyI	GRCGYC	1 cut(s)	337
AfaI	GTAC	7 cut(s)	2078, 2277, 3278, 3628, 4086, 4823, 5079
AfeI	AGCGCT	2 cut(s)	1037, 4145
AflIII	ACRYGT	2 cut(s)	2809, 4248
AhdI	GACNNNNNGTC	1 cut(s)	3049
AjnI	CCWGG	6 cut(s)	466, 585, 901, 1672, 2287, 4111
AjuI	GAANNNNNNNTTGG	4 cut(s)	2401, 2433, 3821, 3853
AloI	GAACNNNNNNTCC	6 cut(s)	2037, 2069, 4047, 4048, 4079, 4080
Alw21I	GWGCWC	3 cut(s)	924, 2307, 4310
Alw26I	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2040, 3528, 3569, 4069
AlwI	GGATC	8 cut(s)	550, 617, 799, 1457, 1962, 2132, 2279, 3740
AlwNI	CAGNNNCTG	3 cut(s)	389, 473, 1187
Ama87I	CYCGRG	1 cut(s)	5276
Aor51HI	AGCGCT	2 cut(s)	1037, 4145
ApaI	GGGCCC	1 cut(s)	2168
ApeKI	GCWGC	9 cut(s)	1187, 1190, 2480, 2498, 2645, 3884, 4685, 4922, 4925
AseI	ATTAAT	1 cut(s)	1493
Asp700I	GAANNNNTTC	3 cut(s)	2332, 2865, 3079
AspLEI	GCGC	5 cut(s)	339, 1038, 4146, 4782, 5110
AsuC2I	CCSGG	1 cut(s)	4039
AsuHPI	GGTGA	7 cut(s)	1400, 1409, 2030, 2087, 2365, 3447, 3673
AsuII	TTCGAA	1 cut(s)	2869
AvaI	CYCGRG	1 cut(s)	5276
BaeGI	GKGCMC	2 cut(s)	2168, 2719
BalI	TGGCCA	3 cut(s)	2914, 3715, 4679
BanI	GGYRCC	2 cut(s)	336, 2971
BanII	GRGCYC	1 cut(s)	2168
BauI	CACGAG	1 cut(s)	687
BbsI	GAAGAC	1 cut(s)	1512
Bbv12I	GWGCWC	3 cut(s)	924, 2307, 4310
BbvI	GCAGC	9 cut(s)	1174, 1177, 2467, 2510, 2632, 3871, 4697, 4909, 4937
BceAI	ACGGC	6 cut(s)	428, 461, 988, 1311, 3803, 4724
BciT130I	CCWGG	6 cut(s)	468, 587, 903, 1674, 2289, 4113
BciVI	GTATCC	2 cut(s)	807, 3408
BclI	TGATCA	3 cut(s)	2020, 2071, 4336
BcnI	CCSGG	1 cut(s)	4039
BcoDI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2040, 3528, 3569, 4069
BfmI	CTRYAG	7 cut(s)	703, 1359, 1800, 4131, 4219, 4281, 4923
BfoI	RGCGCY	4 cut(s)	340, 1039, 4147, 5111
BfuI	GTATCC	2 cut(s)	807, 3408
BglII	AGATCT	3 cut(s)	500, 4787, 4963
BmcAI	AGTACT	2 cut(s)	3628, 4086
Bme1390I	CCNGG	7 cut(s)	468, 587, 903, 1674, 2289, 4039, 4113
BmeRI	GACNNNNNGTC	1 cut(s)	3049
BmeT110I	CYCGRG	1 cut(s)	5276
BmiI	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2166, 2419, 2973, 3045, 4722
BmrFI	CCNGG	7 cut(s)	468, 587, 903, 1674, 2289, 4039, 4113
BmrI	ACTGGG	3 cut(s)	2755, 2796, 5118
BmuI	ACTGGG	3 cut(s)	2755, 2796, 5118
BpiI	GAAGAC	1 cut(s)	1512
BplI	GAGNNNNNCTC	4 cut(s)	47, 79, 3833, 3865
BpmI	CTGGAG	5 cut(s)	418, 938, 3180, 4250, 5187
Bpu14I	TTCGAA	1 cut(s)	2869
BpuEI	CTTGAG	2 cut(s)	2537, 3011
BpuMI	CCSGG	1 cut(s)	4039
BsaAI	YACGTR	1 cut(s)	1213
BsaBI	GATNNNNATC	2 cut(s)	2914, 4962
BsaHI	GRCGYC	1 cut(s)	337
BsaI	GGTCTC	2 cut(s)	151, 2040
BsaWI	WCCGGW	1 cut(s)	2232
BsaXI	ACNNNNNCTCC	6 cut(s)	1849, 1879, 4348, 4378, 4713, 4743
Bse1I	ACTGG	9 cut(s)	1288, 1752, 2761, 2791, 3961, 4082, 4781, 5124, 5180
Bse3DI	GCAATG	3 cut(s)	870, 2339, 4640
Bse8I	GATNNNNATC	2 cut(s)	2914, 4962
BseBI	CCWGG	6 cut(s)	468, 587, 903, 1674, 2289, 4113
BseJI	GATNNNNATC	2 cut(s)	2914, 4962
BseMI	GCAATG	3 cut(s)	870, 2339, 4640
BseMII	CTCAG	9 cut(s)	483, 1167, 2475, 2940, 3245, 3347, 3419, 3441, 3507
BseNI	ACTGG	9 cut(s)	1288, 1752, 2761, 2791, 3961, 4082, 4781, 5124, 5180
BseRI	GAGGAG	5 cut(s)	56, 73, 3334, 3857, 4247
BseSI	GKGCMC	2 cut(s)	2168, 2719
BseX3I	CGGCCG	1 cut(s)	976
BseXI	GCAGC	9 cut(s)	1174, 1177, 2467, 2510, 2632, 3871, 4697, 4909, 4937
BseYI	CCCAGC	2 cut(s)	859, 2879
BsgI	GTGCAG	1 cut(s)	3021
Bsh1236I	CGCG	3 cut(s)	75, 1146, 2710
Bsh1285I	CGRYCG	1 cut(s)	979
BshNI	GGYRCC	2 cut(s)	336, 2971
BsiEI	CGRYCG	1 cut(s)	979
BsiHKAI	GWGCWC	3 cut(s)	924, 2307, 4310
BsiHKCI	CYCGRG	1 cut(s)	5276
BsiSI	CCGG	4 cut(s)	451, 1868, 2233, 4038
BslFI	GGGAC	3 cut(s)	74, 647, 4734
BsmAI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2040, 3528, 3569, 4069
BsmBI	CGTCTC	3 cut(s)	85, 1262, 3528
BsmFI	GGGAC	3 cut(s)	74, 647, 4734
BsmI	GAATGC	2 cut(s)	1441, 3910
Bso31I	GGTCTC	2 cut(s)	151, 2040
BsoBI	CYCGRG	1 cut(s)	5276
Bsp119I	TTCGAA	1 cut(s)	2869
Bsp120I	GGGCCC	1 cut(s)	2164
Bsp1286I	GDGCHC	5 cut(s)	924, 2168, 2307, 2719, 4310
Bsp1407I	TGTACA	1 cut(s)	4821
BspCNI	CTCAG	9 cut(s)	484, 1168, 2476, 2941, 3244, 3348, 3418, 3440, 3506
BspFNI	CGCG	3 cut(s)	75, 1146, 2710
BspHI	TCATGA	2 cut(s)	3094, 4339
BspLI	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2166, 2419, 2973, 3045, 4722
BspMAI	CTGCAG	1 cut(s)	4927
BspPI	GGATC	8 cut(s)	550, 617, 799, 1457, 1962, 2132, 2279, 3740
BspQI	GCTCTTC	2 cut(s)	1944, 5262
BspT104I	TTCGAA	1 cut(s)	2869
BspT107I	GGYRCC	2 cut(s)	336, 2971
BspTNI	GGTCTC	2 cut(s)	151, 2040
BsrBI	CCGCTC	1 cut(s)	105
BsrDI	GCAATG	3 cut(s)	870, 2339, 4640
BsrGI	TGTACA	1 cut(s)	4821
BsrI	ACTGG	9 cut(s)	1288, 1752, 2761, 2791, 3961, 4082, 4781, 5124, 5180
BssNI	GRCGYC	1 cut(s)	337
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	9 cut(s)	518, 1476, 1845, 2041, 2967, 3070, 3858, 4674, 4680
Bst2BI	CACGAG	1 cut(s)	687
Bst2UI	CCWGG	6 cut(s)	468, 587, 903, 1674, 2289, 4113
Bst6I	CTCTTC	7 cut(s)	1944, 2693, 3064, 3655, 3712, 5015, 5262
BstACI	GRCGYC	1 cut(s)	337
BstAPI	GCANNNNNTGC	2 cut(s)	2444, 3008
BstAUI	TGTACA	1 cut(s)	4821
BstBAI	YACGTR	1 cut(s)	1213
BstBI	TTCGAA	1 cut(s)	2869
BstC8I	GCNNGC	7 cut(s)	2363, 2503, 2625, 3266, 4018, 4422, 4939
BstDSI	CCRYGG	1 cut(s)	2708
BstENI	CCTNNNNNAGG	2 cut(s)	4110, 4946
BstFNI	CGCG	3 cut(s)	75, 1146, 2710
BstH2I	RGCGCY	4 cut(s)	340, 1039, 4147, 5111
BstHHI	GCGC	5 cut(s)	339, 1038, 4146, 4782, 5110
BstMAI	GTCTC	9 cut(s)	85, 151, 400, 1172, 1262, 2040, 3528, 3569, 4069
BstMCI	CGRYCG	1 cut(s)	979
BstNI	CCWGG	6 cut(s)	468, 587, 903, 1674, 2289, 4113
BstNSI	RCATGY	5 cut(s)	1713, 2498, 2813, 4252, 4922
BstSCI	CCNGG	7 cut(s)	466, 585, 901, 1672, 2287, 4037, 4111
BstSFI	CTRYAG	7 cut(s)	703, 1359, 1800, 4131, 4219, 4281, 4923
BstSLI	GKGCMC	2 cut(s)	2168, 2719
BstUI	CGCG	3 cut(s)	75, 1146, 2710
BstV1I	GCAGC	9 cut(s)	1174, 1177, 2467, 2510, 2632, 3871, 4697, 4909, 4937
BstV2I	GAAGAC	1 cut(s)	1512
BstX2I	RGATCY	5 cut(s)	500, 542, 3732, 4787, 4963
BstXI	CCANNNNNNTGG	1 cut(s)	4388
BstYI	RGATCY	5 cut(s)	500, 542, 3732, 4787, 4963
BstZI	CGGCCG	1 cut(s)	976
BsuI	GTATCC	2 cut(s)	807, 3408
BtgI	CCRYGG	1 cut(s)	2708
BtgZI	GCGATG	1 cut(s)	4971
BtsI	GCAGTG	3 cut(s)	1633, 2991, 3881
Cac8I	GCNNGC	7 cut(s)	2363, 2503, 2625, 3266, 4018, 4422, 4939
CaiI	CAGNNNCTG	3 cut(s)	389, 473, 1187
CciI	TCATGA	2 cut(s)	3094, 4339
CciNI	GCGGCCGC	1 cut(s)	976
CfoI	GCGC	5 cut(s)	339, 1038, 4146, 4782, 5110
Cfr42I	CCGCGG	1 cut(s)	2711
CseI	GACGC	2 cut(s)	64, 4413
CsiI	ACCWGGT	1 cut(s)	901
Csp6I	GTAC	7 cut(s)	2077, 2276, 3277, 3627, 4085, 4822, 5078
CspCI	CAANNNNNGTGG	2 cut(s)	4117, 4152
CviQI	GTAC	7 cut(s)	2077, 2276, 3277, 3627, 4085, 4822, 5078
DinI	GGCGCC	1 cut(s)	338
DraI	TTTAAA	3 cut(s)	2895, 3390, 4413
DriI	GACNNNNNGTC	1 cut(s)	3049
EaeI	YGGCCR	5 cut(s)	976, 1324, 2912, 3713, 4677
EagI	CGGCCG	1 cut(s)	976
Eam1104I	CTCTTC	7 cut(s)	1944, 2693, 3064, 3655, 3712, 5015, 5262
Eam1105I	GACNNNNNGTC	1 cut(s)	3049
EarI	CTCTTC	7 cut(s)	1944, 2693, 3064, 3655, 3712, 5015, 5262
EclXI	CGGCCG	1 cut(s)	976
Eco130I	CCWWGG	9 cut(s)	518, 1476, 1845, 2041, 2967, 3070, 3858, 4674, 4680
Eco147I	AGGCCT	1 cut(s)	2732
Eco24I	GRGCYC	1 cut(s)	2168
Eco31I	GGTCTC	2 cut(s)	151, 2040
Eco32I	GATATC	2 cut(s)	1644, 4638
Eco47III	AGCGCT	2 cut(s)	1037, 4145
Eco52I	CGGCCG	1 cut(s)	976
Eco57I	CTGAAG	5 cut(s)	411, 1151, 1818, 3909, 4997
Eco88I	CYCGRG	1 cut(s)	5276
EcoNI	CCTNNNNNAGG	2 cut(s)	4110, 4946
EcoO109I	RGGNCCY	4 cut(s)	386, 1931, 2165, 4721
EcoRI	GAATTC	2 cut(s)	590, 3075
EcoRII	CCWGG	6 cut(s)	466, 585, 901, 1672, 2287, 4111
EcoRV	GATATC	2 cut(s)	1644, 4638
EcoT14I	CCWWGG	9 cut(s)	518, 1476, 1845, 2041, 2967, 3070, 3858, 4674, 4680
EcoT22I	ATGCAT	2 cut(s)	6, 1441
EcoT38I	GRGCYC	1 cut(s)	2168
EgeI	GGCGCC	1 cut(s)	338
EheI	GGCGCC	1 cut(s)	338
ErhI	CCWWGG	9 cut(s)	518, 1476, 1845, 2041, 2967, 3070, 3858, 4674, 4680
Esp3I	CGTCTC	3 cut(s)	85, 1262, 3528
FalI	AAGNNNNNCTT	6 cut(s)	1615, 1647, 2291, 2323, 2304, 2336
FaqI	GGGAC	3 cut(s)	74, 647, 4734
FauI	CCCGC	3 cut(s)	2463, 3692, 4930
FauNDI	CATATG	1 cut(s)	1257
FbaI	TGATCA	3 cut(s)	2020, 2071, 4336
FblI	GTMKAC	2 cut(s)	1799, 4893
FriOI	GRGCYC	1 cut(s)	2168
GlaI	GCGC	5 cut(s)	338, 1037, 4145, 4781, 5109
GsaI	CCCAGC	2 cut(s)	863, 2883
GsuI	CTGGAG	5 cut(s)	418, 938, 3180, 4250, 5187
HaeII	RGCGCY	4 cut(s)	340, 1039, 4147, 5111
HapII	CCGG	4 cut(s)	451, 1868, 2233, 4038
HgaI	GACGC	2 cut(s)	64, 4413
HhaI	GCGC	5 cut(s)	339, 1038, 4146, 4782, 5110
Hin1I	GRCGYC	1 cut(s)	337
Hin6I	GCGC	5 cut(s)	337, 1036, 4144, 4780, 5108
HinP1I	GCGC	5 cut(s)	337, 1036, 4144, 4780, 5108
HincII	GTYRAC	4 cut(s)	940, 1353, 3540, 4671
HindII	GTYRAC	4 cut(s)	940, 1353, 3540, 4671
HindIII	AAGCTT	4 cut(s)	251, 2047, 3891, 4989
HpaI	GTTAAC	1 cut(s)	3540
HpaII	CCGG	4 cut(s)	451, 1868, 2233, 4038
HphI	GGTGA	7 cut(s)	1400, 1409, 2030, 2087, 2365, 3447, 3673
Hpy166II	GTNNAC	8 cut(s)	206, 940, 1353, 1800, 2801, 3540, 4671, 4894
Hpy8I	GTNNAC	8 cut(s)	206, 940, 1353, 1800, 2801, 3540, 4671, 4894
Hpy99I	CGWCG	5 cut(s)	80, 1861, 3818, 4429, 5223
HpyCH4IV	ACGT	2 cut(s)	1212, 4024
HpySE526I	ACGT	2 cut(s)	1212, 4024
Hsp92I	GRCGYC	1 cut(s)	337
HspAI	GCGC	5 cut(s)	337, 1036, 4144, 4780, 5108
KasI	GGCGCC	1 cut(s)	336
Ksp22I	TGATCA	3 cut(s)	2020, 2071, 4336
KspAI	GTTAAC	1 cut(s)	3540
KspI	CCGCGG	1 cut(s)	2711
LguI	GCTCTTC	2 cut(s)	1944, 5262
LmnI	GCTCC	9 cut(s)	110, 919, 1014, 1216, 2012, 2302, 2435, 3509, 4218
Lsp1109I	GCAGC	9 cut(s)	1174, 1177, 2467, 2510, 2632, 3871, 4697, 4909, 4937
MabI	ACCWGGT	1 cut(s)	901
MaeII	ACGT	2 cut(s)	1212, 4024
MbiI	CCGCTC	1 cut(s)	105
MfeI	CAATTG	5 cut(s)	1052, 1371, 1680, 1884, 4845
MflI	RGATCY	5 cut(s)	500, 542, 3732, 4787, 4963
MhlI	GDGCHC	5 cut(s)	924, 2168, 2307, 2719, 4310
MlsI	TGGCCA	3 cut(s)	2914, 3715, 4679
MluNI	TGGCCA	3 cut(s)	2914, 3715, 4679
Mly113I	GGCGCC	1 cut(s)	337
MlyI	GAGTC	7 cut(s)	53, 56, 629, 2311, 4337, 4763, 5249
MmeI	TCCRAC	5 cut(s)	489, 1554, 1672, 3749, 4407
Mox20I	TGGCCA	3 cut(s)	2914, 3715, 4679
Mph1103I	ATGCAT	2 cut(s)	6, 1441
MroXI	GAANNNNTTC	3 cut(s)	2332, 2865, 3079
MscI	TGGCCA	3 cut(s)	2914, 3715, 4679
MslI	CAYNNNNRTG	6 cut(s)	1550, 2100, 2856, 3201, 3915, 4512
Msp20I	TGGCCA	3 cut(s)	2914, 3715, 4679
MspA1I	CMGCKG	3 cut(s)	981, 2710, 4928
MspI	CCGG	4 cut(s)	451, 1868, 2233, 4038
MspR9I	CCNGG	7 cut(s)	468, 587, 903, 1674, 2289, 4039, 4113
MunI	CAATTG	5 cut(s)	1052, 1371, 1680, 1884, 4845
Mva1269I	GAATGC	2 cut(s)	1441, 3910
MvaI	CCWGG	6 cut(s)	468, 587, 903, 1674, 2289, 4113
MvnI	CGCG	3 cut(s)	75, 1146, 2710
NarI	GGCGCC	1 cut(s)	337
NciI	CCSGG	1 cut(s)	4039
NdeI	CATATG	1 cut(s)	1257
NlaIV	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2166, 2419, 2973, 3045, 4722
NmeAIII	GCCGAG	1 cut(s)	64
NmuCI	GTSAC	6 cut(s)	2314, 3371, 3749, 4120, 4982, 5143
NotI	GCGGCCGC	1 cut(s)	976
NsiI	ATGCAT	2 cut(s)	6, 1441
NspI	RCATGY	5 cut(s)	1713, 2498, 2813, 4252, 4922
NspV	TTCGAA	1 cut(s)	2869
PaeR7I	CTCGAG	1 cut(s)	5276
PagI	TCATGA	2 cut(s)	3094, 4339
PceI	AGGCCT	1 cut(s)	2732
PciI	ACATGT	2 cut(s)	2809, 4248
PciSI	GCTCTTC	2 cut(s)	1944, 5262
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PctI	GAATGC	2 cut(s)	1441, 3910
PdmI	GAANNNNTTC	3 cut(s)	2332, 2865, 3079
PflMI	CCANNNNNTGG	1 cut(s)	2414
PfoI	TCCNGGA	1 cut(s)	2287
PleI	GAGTC	7 cut(s)	52, 56, 629, 2311, 4336, 4763, 5249
PluTI	GGCGCC	1 cut(s)	340
PpsI	GAGTC	7 cut(s)	52, 56, 629, 2311, 4336, 4763, 5249
Ppu21I	YACGTR	1 cut(s)	1213
PpuMI	RGGWCCY	3 cut(s)	386, 1931, 4721
PscI	ACATGT	2 cut(s)	2809, 4248
PshBI	ATTAAT	1 cut(s)	1493
PsiI	TTATAA	1 cut(s)	3462
Psp5II	RGGWCCY	3 cut(s)	386, 1931, 4721
Psp6I	CCWGG	6 cut(s)	466, 585, 901, 1672, 2287, 4111
PspFI	CCCAGC	2 cut(s)	859, 2879
PspGI	CCWGG	6 cut(s)	466, 585, 901, 1672, 2287, 4111
PspN4I	GGNNCC	9 cut(s)	338, 771, 1218, 1620, 2166, 2419, 2973, 3045, 4722
PspOMI	GGGCCC	1 cut(s)	2164
PspPPI	RGGWCCY	3 cut(s)	386, 1931, 4721
PspXI	VCTCGAGB	1 cut(s)	5276
PsrI	GAACNNNNNNTAC	4 cut(s)	1783, 1815, 4108, 4140
PstI	CTGCAG	1 cut(s)	4927
PstNI	CAGNNNCTG	3 cut(s)	389, 473, 1187
PsuI	RGATCY	5 cut(s)	500, 542, 3732, 4787, 4963
PvuII	CAGCTG	1 cut(s)	4928
RsaI	GTAC	7 cut(s)	2078, 2277, 3278, 3628, 4086, 4823, 5079
RsaNI	GTAC	7 cut(s)	2077, 2276, 3277, 3627, 4085, 4822, 5078
RseI	CAYNNNNRTG	6 cut(s)	1550, 2100, 2856, 3201, 3915, 4512
SacII	CCGCGG	1 cut(s)	2711
SapI	GCTCTTC	2 cut(s)	1944, 5262
ScaI	AGTACT	2 cut(s)	3628, 4086
SchI	GAGTC	7 cut(s)	53, 56, 629, 2311, 4337, 4763, 5249
ScrFI	CCNGG	7 cut(s)	468, 587, 903, 1674, 2289, 4039, 4113
SduI	GDGCHC	5 cut(s)	924, 2168, 2307, 2719, 4310
SexAI	ACCWGGT	1 cut(s)	901
SfcI	CTRYAG	7 cut(s)	703, 1359, 1800, 4131, 4219, 4281, 4923
SfoI	GGCGCC	1 cut(s)	338
Sfr274I	CTCGAG	1 cut(s)	5276
Sfr303I	CCGCGG	1 cut(s)	2711
SfuI	TTCGAA	1 cut(s)	2869
SgrBI	CCGCGG	1 cut(s)	2711
SlaI	CTCGAG	1 cut(s)	5276
SmiMI	CAYNNNNRTG	6 cut(s)	1550, 2100, 2856, 3201, 3915, 4512
SmlI	CTYRAG	3 cut(s)	2516, 3026, 5276
SmoI	CTYRAG	3 cut(s)	2516, 3026, 5276
SseBI	AGGCCT	1 cut(s)	2732
SspDI	GGCGCC	1 cut(s)	336
SspI	AATATT	7 cut(s)	280, 956, 1905, 3549, 3780, 4444, 4489
StuI	AGGCCT	1 cut(s)	2732
StyD4I	CCNGG	7 cut(s)	466, 585, 901, 1672, 2287, 4037, 4111
StyI	CCWWGG	9 cut(s)	518, 1476, 1845, 2041, 2967, 3070, 3858, 4674, 4680
TaiI	ACGT	2 cut(s)	1215, 4027
TaqI	TCGA	7 cut(s)	638, 1433, 2400, 2869, 3167, 5218, 5277
TaqII	GACCGA	2 cut(s)	432, 2022
TatI	WGTACW	6 cut(s)	2076, 2275, 3626, 4084, 4821, 5077
TauI	GCSGC	4 cut(s)	978, 981, 2710, 4166
TseFI	GTSAC	6 cut(s)	2314, 3371, 3749, 4120, 4982, 5143
TseI	GCWGC	9 cut(s)	1187, 1190, 2480, 2498, 2645, 3884, 4685, 4922, 4925
Tsp45I	GTSAC	6 cut(s)	2314, 3371, 3749, 4120, 4982, 5143
TspGWI	ACGGA	4 cut(s)	76, 4518, 4970, 5044
Van91I	CCANNNNNTGG	1 cut(s)	2414
VspI	ATTAAT	1 cut(s)	1493
XagI	CCTNNNNNAGG	2 cut(s)	4110, 4946
XceI	RCATGY	5 cut(s)	1713, 2498, 2813, 4252, 4922
XhoI	CTCGAG	1 cut(s)	5276
XmiI	GTMKAC	2 cut(s)	1799, 4893
XmnI	GAANNNNTTC	3 cut(s)	2332, 2865, 3079
ZrmI	AGTACT	2 cut(s)	3628, 4086
Zsp2I	ATGCAT	2 cut(s)	6, 1441

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.