Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006814

Physical Location & Seq

Genomic Coordinates Reverse (-)

3209518 .. 3222892

Gene Structure 13375 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1040600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 5433 bp

ATGCATCTCTACAATGCATGGCTGCCGCCACCGGTGGCCGAGGAGTCGAAGAAAGAGAAGGAGTCCTTCTCCTGGGTCGTCTCCTCCGTCAAGAACTCGTACAAACCCGACGACCCTGAATCCGTCTATGCTACTCTCAAATGGGTTTCTGTTATCGACCTTTTCGTCAAGGCGAAAAGTGAAGTATCCTTGGAAGATGTAACTGCCCTTGTTGAATTTGGATTGGAATTGTTTTATGTATCGCAAAATAAGCTTTATGCCCAGGTTAGATGGGGAAACATCCTAGTCAAACTACTCAATAAGCATCGAAAGAAGTTGTCTTTGAAATTAAAATGGCGGCCTTTGTACGATACATTGATCCATACACATTTCACTAGGAATACAGGTCCAGAAGGCTGGAGATTGAGACAACGGCATTTTGAGGCTACTACTTCTCTAGTTCGGTCCTGCCGGAAGTTCTTCCCACCAGGTTCTGCCTTTGAGATATGGTCCGAGTTTAGATCTTTATTGGAAAATCCCTGGCATAATTCATCCTTTGAGGGATCCGGGTTTGTTAGACTGTTTCTTCCTACAAACTTGGACAACCAGGACTTCTTCTCGCACGATTGGATTAAAGAGTTTTTCCGCCTCTGGGACTCGATACCGAATTGCCAATTTTGGAACAGTCAATGGGCGGCTGTTATAGCTCGTGTTGTTAAGAACTACAAGTTGATTGATTGGGAGTGTTACTTGCCCACACTTTTCACCAAATACTTAAATATGTTTGAGGTTCCTGTGGCAAATGGAGGTGGATCATATCCATTCTCTGTGGATGTTCCAAGAAACACAAGATTCTTGTTCTCCAATAAGACAGTTACCCCAGCAAAGGCCATCGCAAAGTCCATTGTCAATTTGCTAAGGCCTGGTAGCTCAGCACAGCAGCATTTTGAAAAATTGGTCAATCTCTTAGAACAGTATTACCATCCTTCTAATGGTGGTCGCTGGACTTATGCATTAGAACGTTTTTTGCTCTATTTGGTAATTACATTCCAGAAACGCTTACAGCGTGAACAACTCAACATTGATAAGGATGGACAGGATGAACAGTACCTAGGAAGATCAGAAAGGACGTTCTTTGTCAAGGTGGTGCTCAAGTTAATTGATCGTGGTCAATATAGCAAGAATGAACATCTTTCTGAAACAGTTGCTGCTGCAACTTCAATTTTGTCTTATGTGGAGCCTACTCTCGTCCTTCCTTTTGTCGCATCTCGCTTCCAAATGGCCTTGGAGACGATGACTGCCACCCACCAGTTGCAGATTGCAGTGACGTCGGTAGCATTTGTTGGGCGTTCATTGTTCCTCACATCCCTATCAAAATCAGTTGATCCTGGTAATGGTGATGAGTTCATAGAGCTTTTGATGGTTTCACTATCCAACGCATTACTTGGTATGGATGCCAATGATCCTCCTAAAACCTTAGCAACAATGCAATTAATTGGCTCTATATTTTCCAATATGTCTTCATTGGATGATAATATTGATGAGCTATCAGTCATGCCAATGATTAGGTTTTCTGAATGGTTAGATGAGTTCTTGTGTCGTCTATTTTCCTTACTCTTACACCTGGAACCAAGCAGTGTTACGAATGAAGGCCTACATTCATCAGCAGTGTCAGGAACTTTTCTAGTTGAGGATGGTCCTTACTACTATTGCATGCTTGAAATCTTGCTCGGGAGACTTTCACAACCTCTATATAATCAGGCTTTGAAGAAGATCTCCAAGTTTGTGAAGACAAATATTCTTCCTGGGGCAATTTCAGAGGTTGGACTGCTTTGTTGTGCATGCGTTCATTCCAACCCAGAAGAAGCGGTTACTCAACTTGTCAAGCCAGTTTTATTGTCTGTTATTTCTTCTTTGGAAGGAACACCAGCTACAGGATTTGGAGGTAGAGGAATATGTGACACTTCAGTGTCAACTAAGGTTAAGCCAACAATTTCTCCAGCTCTTGAAACAGCAATTGACTATCAATTAAAAGTGGTATCAGTTGCCATAAGTTATGGAGGTCCTGCACTTCTGCGCTACAAGGATCACTTCAAAGAGGCTATACTTTCTGCTTTTGAGTCCCCATCTTGGAAGGTCAATGGAGCTGGTGATCATCTTCTTCGATCTCTCCTTGGAAGCCTGGTTCTTTATTATCCTATTGATCAGTACAAGTGTGTTTTGCATCATCGAAATGCTGCTGCATTAGAGGAATGGATCAGCACCAAAGATTACTTGAATGATAAACCATTGGCGGCCCCCAAGTGGCATATCCCAAGTGTCGAAGAAGTTGAATTTGCAAATGAACTCTTGGACTTTCATTTTCGGATGGCTTTGGATGATCTTTCGAGAATATGTGAAACTAAACTCCACTCTGATCCAGGAGATGAGAAAGATCACTTGAAAGTGACTCTTCTACGTATAGATTCTTCATTGCAAGGTGTTTTGTCTTGTTTGCCTGACTTCGCCCCGTCCTCAAGGAATGGGACTGTTGAACATCCAAATCATGCTGCTTTTCTAATAGCTGGAGCAACAGGTTCAACTGTTGGTACCACTAAATTGCGGGAAAAAGCTGCTGACATTATACATGCAGCCTGCAAATACATACTAGAGAAAAAATCAGATGATAGCATTCTGTTGATACTCATTATTCGTATTATGGATGCTTTAGGAAACTATGGAAGTTCGGAATATGATGAGTGGTCAAATCACAGGCAGGCATGGAAACTAGAATCTGCTGCCATTGTAGAACCTTCAGTAAACTTTATAGTTTCAGCTCATTCTAAGGGGAAGAGAAGGCCGCGGTGGGCACTCATTGACAAGGCATTCATGCACAGTACTTGGAGATCATCACAATCATCTTATCACATATATCGTACTAATGGGAATTTCGGTCCACCAGACCATGTGAATCTTTTGGTGGATAATCTTCTAAATCTCTCATTGCATAGCTATGCAACTGTTCGTTTACTTGCTGGAAAATCTCTTTTAAAGATGATAAAGAGATGGCCATCCATGATTTCAAAGTGCGTCCTTTCTCTAACAGAGAATTTACGGAATCCCAAGTCACCAGAATATGCGGTGCTAGGTTCATGTGCAGTCCTTGCCACGCAGACAGTTCTCAAGCATTTGACAATGGACCCAAAGGCGTTCTCTTCTTGTATTCTTGGAATTCTTTCAAGCTCTCATCATGAATCACTGAAAACCCAGAAAGCAATCAATGAGCTTTTTGTCAAATACAACATCCACTTTGCTGGAGTATCTAGAAGCATTTTCAGGGCATCAGGCAATCATACAGAAGCACCAGATTTTGCAGATTTGGTTTCTCAGATCACTTCTATGAGTTTTGAATCTACCGGCTTGCATTGGAGGTACAATCTGATGGCTAATAGGGTTCTACTCTTGTTGGCTATGTCATCTAGGAATGATCCAACTTCCTCCTCAAAAATTTTGAGTGAAACTGCAGGTCACTTCTTAAAGAATTTAAAAAGTCAGCTTCCTCAGACAAGGATACTTGCAATTTCAGCTTTAAATACACTGTTAAAAGAATCACCTTATAAACTGTCTGCTGGGGAACAGACTCTTCCCTCTGGGAATTTGCAAGGAAGTAAAAAGTCATCCCTTGAAGGAGAATTAACGCAAATATTTCAGGAAGATGGTTTTTTCAATGAGACCTTGAACAGTCTTTCTCATGTTCATATTGTTACTGATGCTGAAAGTGCATCTTCTAGAGGAAATCATGGATCTTTTCAGAGCCTAGCAGACAAATCAATCACCCGATTTTATTTTGATTTCACCGCTTCATGGCCACGCACTCCTACTTGGATCTCTTTATTAGGGAGTGACACATTTTACTCAAACTTTGCGAGAATTTTCAAGCGGCTAATACAGGAATGTGGCATACCTGTTCTATTGTCACTGAAAAGCTCATTAGAGGAGTTTGTAAATGCCAAGGAGAGGTCTAAGCAGTGTGTTGCTGCTGAAGCATTGGCTGGAATATTGCATTCTGATGTTGACGGTATTTTAGTGGCATGGGACACTTGGATTTTGGCCCAGTTGCAGAATATCATCGTATCACAATCAGTAGAGTCAATACCTGAGTGGGCAGCCTGTATACGTTACGCTGTTACTGGGAAAGGGAAGCATGGAACGAGAGTTCCTGTTTTGAGGCAACCAGTACTGGATTGTTTGGCCACACCTTTAACTGGAACTGTAACCACGACTGTAGTTGCCAAGCGCTATGCTTTTCTTTCGGCTGCACTTATAGAATTATCTCCACAGACAATGCCGTTCACTGAAGTACAGCTCCATTATAGACTTCTGGAGGAGCTGCTTAGTAGCATGTGCCATTCATCTGCCCAGGTAAGAGAAGCTTTAGGTGTTACTCTTGCAGTGTTGTGCTCTAACATCCAGCTTTATGAGTCATTTAATCATGAGCATTCACATGAGGGGGAAAAAAGAGATGTTGCCTACAAATTTGATGGGAAAAGTTGGGTTCAGTTTCTGAAAGAGAGAGCATCTGAAGTGCTGATGAATATTCAGAATACTTCCCAGTCTGACAGTTTGGAGACCGCAACAAATACTAGTTCTGAGAATGGGAATTCCAATAGTGATTCACAGGATGATATCAAGTGGATGGAGACGCTTTTCCATTTTATCATCTCATCTTTGAAGTCTGGGCGAATTTCATATCTTCTGGATGTTATTGTGGGACTTCTTTCTCCTGTAATATCCTTACAGGAAACATCAAGTAAAGATCTGTCAACCTTAGCGAAAGTAGCTTTTGAATTGCTGAAGTGGAGAATTTTCTGTGGACCTCATCTCCAGGAGGCTGTATCTGTGATCCTTTCTTCTGCCAATGACTCTAACTGGCGAATTAGATCTGCAACACTTACATATCTGCGAACTTTTATGTATAGGCACAGTTTCGTTCTCTCAAATGCGAAGAAGCAGCAAATTTGGAGAACTGTTGAAAATCTACTTTTAGACAACCAAGTTGAGGTAAGAGAGCATGCTGCTGCAGTTTTAGCGGGCCTAATGAAGGGTGGGGATGAAGATCTTGCTAAAGACTTTCGCGATAAAGCTTATACAGAAGCTACTATTCTTCAGAGGAAGAGAAAGCGGAGAACTTTGAGCTCAAGCCAGTCCATAGCTTCTATTCACGGTGCTGTACTTGCTTTGGTAGCTTCCGTGTTGTCAGCCCCGTATGATATGCCCAGTTGGTTGCCCGAGCACGTTACTTTACTTGCTCGTTTTAGTGGAGAGCCATCGCCGGTGAAATCTACGGTTACAAAAGCAGTTGCAGAGTTTCGACGTACACATGCTGATACATGGAATTTTCAGAAAGATTCATTTACTGAAGAGCAACTTGAGGTTCTGGCTGATACGTCGTCTTCATCGTCGTATTTCGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000502 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0005839 GO:0006508 GO:0006807 GO:0008047 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009893 GO:0009987 GO:0010498 GO:0010499 GO:0010604 GO:0010952 GO:0016504 GO:0019222 GO:0019538 GO:0030162 GO:0030163 GO:0030234 GO:0031323 GO:0031325 GO:0032268 GO:0032270 GO:0032991 GO:0042393 GO:0043085 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044093 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0044877 GO:0045862 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051173 GO:0051246 GO:0051247 GO:0051336 GO:0051345 GO:0051603 GO:0052547 GO:0060255 GO:0061134 GO:0065007 GO:0065009 GO:0070577 GO:0070628 GO:0071704 GO:0080090 GO:0098772 GO:0140030 GO:0140033 GO:1901564 GO:1901565 GO:1901575 GO:1902494 GO:1905368 GO:1905369

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

BLM10_mid DUF3437

Protein Analysis

1811

Amino Acids

202.94

Weight (kDa)

7.34

Isoelectric Point (pI)

43.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HEAT_PSME4_mid	PF16507	310 - 500	7.9e-30	Proteasome activator complex subunit 4, mid HEAT repeats region
HEAT_PSME4_mid	PF16507	502 - 821	6.6e-19	Proteasome activator complex subunit 4, mid HEAT repeats region
PSME4_C	PF11919	1725 - 1810	3.3e-28	Proteasome activator complex subunit 4-like, C-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Orthologous Genes (Group: OG0006814)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G13330
fragaria_vesca	FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830 FvH4_3g41830
malus_domestica	MD00G1206400.v1.1 MD00G1206500.v1.1 MD00G1206600.v1.1 MD03G1040600.v1.1 MD11G1042000.v1.1
prunus_persica	Prupe.6G032900_v2.0.a1
pyrus_communis	pycom03g03130 pycom11g03610
rosa_chinensis	RchiOBHm_Chr5g0075021
rosa_laevigata	RLG00000036518
rosa_multiflora	Rmu_sc0006984.1_g000002
rosa_rugosa	Rorug05G0435500
rosa_samantha	Rh5AG492800 Rh5BG514100 Rh5CG537700 Rh5DG526400
rosa_wichuraiana	Rw5G045680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3576
AasI	GACNNNNNNGTC	1 cut(s)	164
AatII	GACGTC	1 cut(s)	1310
Acc36I	ACCTGC	1 cut(s)	3473
Acc65I	GGTACC	1 cut(s)	2567
AccB1I	GGYRCC	1 cut(s)	2567
AccI	GTMKAC	1 cut(s)	4132
AccII	CGCG	2 cut(s)	2821, 5094
AclI	AACGTT	1 cut(s)	1000
AcoI	YGGCCR	4 cut(s)	36, 3026, 3824, 4209
AcuI	CTGAAG	8 cut(s)	1929, 2757, 4020, 4335, 4560, 4832, 5108, 5397
AcyI	GRCGYC	1 cut(s)	1307
AfeI	AGCGCT	1 cut(s)	4256
AgeI	ACCGGT	1 cut(s)	31
AhlI	ACTAGT	1 cut(s)	4601
AleI	CACNNNNGTG	1 cut(s)	1946
AloI	GAACNNNNNNTCC	6 cut(s)	2148, 2180, 4158, 4159, 4190, 4191
Alw21I	GWGCWC	4 cut(s)	1131, 4421, 5156, 5253
Alw26I	GTCTC	7 cut(s)	85, 400, 1262, 1708, 3683, 4580, 4652
AlwNI	CAGNNNCTG	3 cut(s)	473, 1187, 4522
Ama87I	CYCGRG	2 cut(s)	1709, 5246
Aor51HI	AGCGCT	1 cut(s)	4256
AseI	ATTAAT	1 cut(s)	1472
AsiGI	ACCGGT	1 cut(s)	31
Asp700I	GAANNNNTTC	2 cut(s)	458, 3193
Asp718I	GGTACC	1 cut(s)	2567
AspA2I	CCTAGG	1 cut(s)	1090
AspLEI	GCGC	2 cut(s)	2058, 4257
AsuC2I	CCSGG	1 cut(s)	547
AsuHPI	GGTGA	8 cut(s)	736, 1388, 2141, 3078, 3561, 3784, 3805, 5305
AvaI	CYCGRG	2 cut(s)	1709, 5246
AvaII	GGWCC	8 cut(s)	386, 444, 489, 1676, 2042, 2912, 3157, 4832
AvrII	CCTAGG	1 cut(s)	1090
BaeGI	GKGCMC	1 cut(s)	2830
BalI	TGGCCA	3 cut(s)	3028, 3826, 4211
BamHI	GGATCC	1 cut(s)	542
BanI	GGYRCC	1 cut(s)	2567
BanII	GRGCYC	1 cut(s)	5156
BarI	GAAGNNNNNNTAC	2 cut(s)	2431, 2463
BauI	CACGAG	1 cut(s)	687
BbsI	GAAGAC	3 cut(s)	1491, 1775, 5403
Bbv12I	GWGCWC	4 cut(s)	1131, 4421, 5156, 5253
BceAI	ACGGC	2 cut(s)	428, 4291
BcgI	CGANNNNNNTGC	2 cut(s)	1290, 1324
BciVI	GTATCC	2 cut(s)	196, 3522
BclI	TGATCA	2 cut(s)	2131, 2182
BcnI	CCSGG	1 cut(s)	547
BcoDI	GTCTC	7 cut(s)	85, 400, 1262, 1708, 3683, 4580, 4652
BcuI	ACTAGT	1 cut(s)	4601
BfmI	CTRYAG	4 cut(s)	1911, 3480, 4242, 5037
BfoI	RGCGCY	1 cut(s)	4258
BfuAI	ACCTGC	1 cut(s)	3473
BfuI	GTATCC	2 cut(s)	196, 3522
BglII	AGATCT	5 cut(s)	500, 1752, 4774, 4898, 5074
BlnI	CCTAGG	1 cut(s)	1090
BlpI	GCTNAGC	1 cut(s)	910
BmcAI	AGTACT	2 cut(s)	2857, 4197
Bme18I	GGWCC	8 cut(s)	386, 444, 489, 1676, 2042, 2912, 3157, 4832
BmeT110I	CYCGRG	2 cut(s)	1709, 5246
BmiI	GGNNCC	7 cut(s)	544, 771, 1218, 1608, 2277, 2569, 3159
BmrI	ACTGGG	4 cut(s)	4066, 4158, 4564, 5229
BmuI	ACTGGG	4 cut(s)	4066, 4158, 4564, 5229
BpiI	GAAGAC	3 cut(s)	1491, 1775, 5403
BplI	GAGNNNNNCTC	2 cut(s)	53, 85
BpmI	CTGGAG	6 cut(s)	418, 1962, 2565, 3294, 4361, 4826
Bpu10I	CCTNAGC	3 cut(s)	896, 1456, 4786
Bpu1102I	GCTNAGC	1 cut(s)	910
BpuEI	CTTGAG	5 cut(s)	1115, 2479, 3125, 5140, 5408
BpuMI	CCSGG	1 cut(s)	547
BsaAI	YACGTR	1 cut(s)	2438
BsaBI	GATNNNNATC	2 cut(s)	3028, 5073
BsaHI	GRCGYC	1 cut(s)	1307
BsaI	GGTCTC	2 cut(s)	3683, 4580
BsaWI	WCCGGW	1 cut(s)	31
BsaXI	ACNNNNNCTCC	4 cut(s)	1960, 1990, 4824, 4854
Bse118I	RCCGGY	3 cut(s)	31, 3374, 5290
Bse3DI	GCAATG	2 cut(s)	2450, 2960
Bse8I	GATNNNNATC	2 cut(s)	3028, 5073
BseJI	GATNNNNATC	2 cut(s)	3028, 5073
BseMI	GCAATG	2 cut(s)	2450, 2960
BseMII	CTCAG	5 cut(s)	924, 3359, 3533, 4107, 4599
BseRI	GAGGAG	5 cut(s)	56, 73, 3448, 3968, 4358
BseSI	GKGCMC	1 cut(s)	2830
BseYI	CCCAGC	2 cut(s)	859, 3587
BsgI	GTGCAG	3 cut(s)	2031, 3135, 4260
Bsh1236I	CGCG	2 cut(s)	2821, 5094
BshNI	GGYRCC	1 cut(s)	2567
BshTI	ACCGGT	1 cut(s)	31
BsiHKAI	GWGCWC	4 cut(s)	1131, 4421, 5156, 5253
BsiHKCI	CYCGRG	2 cut(s)	1709, 5246
BsiSI	CCGG	5 cut(s)	32, 451, 546, 3375, 5291
BslFI	GGGAC	5 cut(s)	647, 2086, 2518, 4067, 4743
BsmAI	GTCTC	7 cut(s)	85, 400, 1262, 1708, 3683, 4580, 4652
BsmBI	CGTCTC	3 cut(s)	85, 1262, 4652
BsmFI	GGGAC	5 cut(s)	647, 2086, 2518, 4067, 4743
BsmI	GAATGC	4 cut(s)	2649, 2843, 4021, 4456
Bso31I	GGTCTC	2 cut(s)	3683, 4580
BsoBI	CYCGRG	2 cut(s)	1709, 5246
Bsp1286I	GDGCHC	5 cut(s)	1131, 2830, 4421, 5156, 5253
Bsp1720I	GCTNAGC	1 cut(s)	910
Bsp68I	TCGCGA	1 cut(s)	5094
BspCNI	CTCAG	5 cut(s)	923, 3358, 3532, 4108, 4600
BspFNI	CGCG	2 cut(s)	2821, 5094
BspHI	TCATGA	2 cut(s)	3208, 4450
BspLI	GGNNCC	7 cut(s)	544, 771, 1218, 1608, 2277, 2569, 3159
BspMAI	CTGCAG	2 cut(s)	3484, 5041
BspMI	ACCTGC	1 cut(s)	3473
BspQI	GCTCTTC	1 cut(s)	5373
BspT107I	GGYRCC	1 cut(s)	2567
BspTNI	GGTCTC	2 cut(s)	3683, 4580
BsrDI	GCAATG	2 cut(s)	2450, 2960
BsrFI	RCCGGY	3 cut(s)	31, 3374, 5290
BssAI	RCCGGY	3 cut(s)	31, 3374, 5290
BssNAI	GTATAC	1 cut(s)	4133
BssNI	GRCGYC	1 cut(s)	1307
BssSI	CACGAG	1 cut(s)	687
BssT1I	CCWWGG	5 cut(s)	189, 1090, 1263, 2152, 3969
Bst1107I	GTATAC	1 cut(s)	4133
Bst2BI	CACGAG	1 cut(s)	687
Bst6I	CTCTTC	6 cut(s)	2436, 2804, 3178, 3606, 5126, 5373
BstACI	GRCGYC	1 cut(s)	1307
BstAPI	GCANNNNNTGC	1 cut(s)	3122
BstBAI	YACGTR	1 cut(s)	2438
BstC8I	GCNNGC	7 cut(s)	1694, 1822, 2614, 2736, 3380, 5031, 5050
BstDSI	CCRYGG	1 cut(s)	2819
BstENI	CCTNNNNNAGG	1 cut(s)	5057
BstFNI	CGCG	2 cut(s)	2821, 5094
BstH2I	RGCGCY	1 cut(s)	4258
BstHHI	GCGC	2 cut(s)	2058, 4257
BstMAI	GTCTC	7 cut(s)	85, 400, 1262, 1708, 3683, 4580, 4652
BstNSI	RCATGY	6 cut(s)	1696, 1824, 2609, 4363, 5033, 5342
BstSFI	CTRYAG	4 cut(s)	1911, 3480, 4242, 5037
BstSLI	GKGCMC	1 cut(s)	2830
BstSNI	TACGTA	1 cut(s)	2438
BstUI	CGCG	2 cut(s)	2821, 5094
BstV2I	GAAGAC	3 cut(s)	1491, 1775, 5403
BstX2I	RGATCY	8 cut(s)	500, 542, 1752, 3761, 3843, 4774, 4898, 5074
BstXI	CCANNNNNNTGG	2 cut(s)	396, 3272
BstYI	RGATCY	8 cut(s)	500, 542, 1752, 3761, 3843, 4774, 4898, 5074
BstZ17I	GTATAC	1 cut(s)	4133
BsuI	GTATCC	2 cut(s)	196, 3522
BtgI	CCRYGG	1 cut(s)	2819
BtgZI	GCGATG	2 cut(s)	856, 5271
BtsI	GCAGTG	5 cut(s)	1308, 1621, 1653, 3992, 4416
BtuMI	TCGCGA	1 cut(s)	5094
BveI	ACCTGC	1 cut(s)	3473
Cac8I	GCNNGC	7 cut(s)	1694, 1822, 2614, 2736, 3380, 5031, 5050
CaiI	CAGNNNCTG	3 cut(s)	473, 1187, 4522
CciI	TCATGA	2 cut(s)	3208, 4450
CfoI	GCGC	2 cut(s)	2058, 4257
Cfr10I	RCCGGY	3 cut(s)	31, 3374, 5290
Cfr42I	CCGCGG	1 cut(s)	2822
CseI	GACGC	2 cut(s)	3037, 4669
CsiI	ACCWGGT	1 cut(s)	466
CspAI	ACCGGT	1 cut(s)	31
DraI	TTTAAA	3 cut(s)	3009, 3504, 3549
DrdI	GACNNNNNNGTC	1 cut(s)	164
DseDI	GACNNNNNNGTC	1 cut(s)	164
EaeI	YGGCCR	4 cut(s)	36, 3026, 3824, 4209
Eam1104I	CTCTTC	6 cut(s)	2436, 2804, 3178, 3606, 5126, 5373
EarI	CTCTTC	6 cut(s)	2436, 2804, 3178, 3606, 5126, 5373
EciI	GGCGGA	1 cut(s)	614
Ecl136II	GAGCTC	1 cut(s)	5154
Eco105I	TACGTA	1 cut(s)	2438
Eco130I	CCWWGG	5 cut(s)	189, 1090, 1263, 2152, 3969
Eco147I	AGGCCT	2 cut(s)	901, 1632
Eco24I	GRGCYC	1 cut(s)	5156
Eco31I	GGTCTC	2 cut(s)	3683, 4580
Eco32I	GATATC	1 cut(s)	4645
Eco47I	GGWCC	8 cut(s)	386, 444, 489, 1676, 2042, 2912, 3157, 4832
Eco47III	AGCGCT	1 cut(s)	4256
Eco53kI	GAGCTC	1 cut(s)	5154
Eco57I	CTGAAG	8 cut(s)	1929, 2757, 4020, 4335, 4560, 4832, 5108, 5397
Eco88I	CYCGRG	2 cut(s)	1709, 5246
EcoICRI	GAGCTC	1 cut(s)	5154
EcoNI	CCTNNNNNAGG	1 cut(s)	5057
EcoO109I	RGGNCCY	1 cut(s)	2042
EcoRI	GAATTC	2 cut(s)	3189, 4618
EcoRV	GATATC	1 cut(s)	4645
EcoT14I	CCWWGG	5 cut(s)	189, 1090, 1263, 2152, 3969
EcoT22I	ATGCAT	3 cut(s)	6, 19, 994
EcoT38I	GRGCYC	1 cut(s)	5156
ErhI	CCWWGG	5 cut(s)	189, 1090, 1263, 2152, 3969
Esp3I	CGTCTC	3 cut(s)	85, 1262, 4652
FaqI	GGGAC	5 cut(s)	647, 2086, 2518, 4067, 4743
FauI	CCCGC	2 cut(s)	2574, 5041
FbaI	TGATCA	2 cut(s)	2131, 2182
FblI	GTMKAC	1 cut(s)	4132
FriOI	GRGCYC	1 cut(s)	5156
GlaI	GCGC	2 cut(s)	2057, 4256
GsaI	CCCAGC	2 cut(s)	863, 3591
GsuI	CTGGAG	6 cut(s)	418, 1962, 2565, 3294, 4361, 4826
HaeII	RGCGCY	1 cut(s)	4258
HapII	CCGG	5 cut(s)	32, 451, 546, 3375, 5291
HgaI	GACGC	2 cut(s)	3037, 4669
HhaI	GCGC	2 cut(s)	2058, 4257
Hin1I	GRCGYC	1 cut(s)	1307
Hin6I	GCGC	2 cut(s)	2056, 4255
HinP1I	GCGC	2 cut(s)	2056, 4255
HincII	GTYRAC	3 cut(s)	1953, 4033, 4782
HindII	GTYRAC	3 cut(s)	1953, 4033, 4782
HindIII	AAGCTT	3 cut(s)	251, 4389, 5100
HpaII	CCGG	5 cut(s)	32, 451, 546, 3375, 5291
HphI	GGTGA	8 cut(s)	736, 1388, 2141, 3078, 3561, 3784, 3805, 5305
Hpy99I	CGWCG	5 cut(s)	113, 1312, 5334, 5410, 5422
HpyCH4IV	ACGT	8 cut(s)	1000, 1109, 1307, 2437, 4135, 5253, 5332, 5405
HpySE526I	ACGT	8 cut(s)	1000, 1109, 1307, 2437, 4135, 5253, 5332, 5405
Hsp92I	GRCGYC	1 cut(s)	1307
HspAI	GCGC	2 cut(s)	2056, 4255
KpnI	GGTACC	1 cut(s)	2571
Ksp22I	TGATCA	2 cut(s)	2131, 2182
KspI	CCGCGG	1 cut(s)	2822
LguI	GCTCTTC	1 cut(s)	5373
LmnI	GCTCC	5 cut(s)	1216, 2123, 2546, 4329, 4345
MabI	ACCWGGT	1 cut(s)	466
MaeII	ACGT	8 cut(s)	1000, 1109, 1307, 2437, 4135, 5253, 5332, 5405
MfeI	CAATTG	1 cut(s)	1995
MflI	RGATCY	8 cut(s)	500, 542, 1752, 3761, 3843, 4774, 4898, 5074
MhlI	GDGCHC	5 cut(s)	1131, 2830, 4421, 5156, 5253
MlsI	TGGCCA	3 cut(s)	3028, 3826, 4211
MluNI	TGGCCA	3 cut(s)	3028, 3826, 4211
MlyI	GAGTC	9 cut(s)	53, 71, 629, 2108, 2422, 3592, 4115, 4448, 4874
MmeI	TCCRAC	4 cut(s)	1437, 1783, 1857, 3473
Mox20I	TGGCCA	3 cut(s)	3028, 3826, 4211
Mph1103I	ATGCAT	3 cut(s)	6, 19, 994
MroXI	GAANNNNTTC	2 cut(s)	458, 3193
MscI	TGGCCA	3 cut(s)	3028, 3826, 4211
MslI	CAYNNNNRTG	7 cut(s)	1538, 1946, 2211, 2970, 3356, 4026, 4461
Msp20I	TGGCCA	3 cut(s)	3028, 3826, 4211
MspA1I	CMGCKG	1 cut(s)	2821
MspI	CCGG	5 cut(s)	32, 451, 546, 3375, 5291
MunI	CAATTG	1 cut(s)	1995
Mva1269I	GAATGC	4 cut(s)	2649, 2843, 4021, 4456
MvnI	CGCG	2 cut(s)	2821, 5094
NciI	CCSGG	1 cut(s)	547
NlaIV	GGNNCC	7 cut(s)	544, 771, 1218, 1608, 2277, 2569, 3159
NmeAIII	GCCGAG	1 cut(s)	64
NmuCI	GTSAC	7 cut(s)	1303, 1937, 2425, 3084, 3485, 3860, 3933
NruI	TCGCGA	1 cut(s)	5094
NsiI	ATGCAT	3 cut(s)	6, 19, 994
NspI	RCATGY	6 cut(s)	1696, 1824, 2609, 4363, 5033, 5342
OliI	CACNNNNGTG	1 cut(s)	1946
PaeI	GCATGC	3 cut(s)	1696, 1824, 5033
PagI	TCATGA	2 cut(s)	3208, 4450
PceI	AGGCCT	2 cut(s)	901, 1632
PciSI	GCTCTTC	1 cut(s)	5373
PcsI	WCGNNNNNNNCGW	4 cut(s)	84, 162, 1042, 5414
PctI	GAATGC	4 cut(s)	2649, 2843, 4021, 4456
PdmI	GAANNNNTTC	2 cut(s)	458, 3193
PfoI	TCCNGGA	2 cut(s)	2398, 4842
PinAI	ACCGGT	1 cut(s)	31
PleI	GAGTC	9 cut(s)	52, 70, 629, 2107, 2422, 3592, 4114, 4447, 4874
PpsI	GAGTC	9 cut(s)	52, 70, 629, 2107, 2422, 3592, 4114, 4447, 4874
Ppu21I	YACGTR	1 cut(s)	2438
PpuMI	RGGWCCY	1 cut(s)	2042
PshBI	ATTAAT	1 cut(s)	1472
PsiI	TTATAA	1 cut(s)	3576
Psp124BI	GAGCTC	1 cut(s)	5156
Psp1406I	AACGTT	1 cut(s)	1000
Psp5II	RGGWCCY	1 cut(s)	2042
PspFI	CCCAGC	2 cut(s)	859, 3587
PspN4I	GGNNCC	7 cut(s)	544, 771, 1218, 1608, 2277, 2569, 3159
PspPPI	RGGWCCY	1 cut(s)	2042
PsrI	GAACNNNNNNTAC	2 cut(s)	1894, 1926
PstI	CTGCAG	2 cut(s)	3484, 5041
PstNI	CAGNNNCTG	3 cut(s)	473, 1187, 4522
PsuI	RGATCY	8 cut(s)	500, 542, 1752, 3761, 3843, 4774, 4898, 5074
RruI	TCGCGA	1 cut(s)	5094
RseI	CAYNNNNRTG	7 cut(s)	1538, 1946, 2211, 2970, 3356, 4026, 4461
SacI	GAGCTC	1 cut(s)	5156
SacII	CCGCGG	1 cut(s)	2822
SapI	GCTCTTC	1 cut(s)	5373
ScaI	AGTACT	2 cut(s)	2857, 4197
SchI	GAGTC	9 cut(s)	53, 71, 629, 2108, 2422, 3592, 4115, 4448, 4874
SduI	GDGCHC	5 cut(s)	1131, 2830, 4421, 5156, 5253
SexAI	ACCWGGT	1 cut(s)	466
SfcI	CTRYAG	4 cut(s)	1911, 3480, 4242, 5037
Sfr303I	CCGCGG	1 cut(s)	2822
SgrAI	CRCCGGYG	2 cut(s)	31, 5290
SgrBI	CCGCGG	1 cut(s)	2822
SinI	GGWCC	8 cut(s)	386, 444, 489, 1676, 2042, 2912, 3157, 4832
SmiMI	CAYNNNNRTG	7 cut(s)	1538, 1946, 2211, 2970, 3356, 4026, 4461
SmlI	CTYRAG	5 cut(s)	1130, 2494, 3140, 5155, 5387
SmoI	CTYRAG	5 cut(s)	1130, 2494, 3140, 5155, 5387
SnaBI	TACGTA	1 cut(s)	2438
SpeI	ACTAGT	1 cut(s)	4601
SphI	GCATGC	3 cut(s)	1696, 1824, 5033
SseBI	AGGCCT	2 cut(s)	901, 1632
SspI	AATATT	5 cut(s)	1516, 1777, 3663, 4017, 4555
SstI	GAGCTC	1 cut(s)	5156
StuI	AGGCCT	2 cut(s)	901, 1632
StyI	CCWWGG	5 cut(s)	189, 1090, 1263, 2152, 3969
TaiI	ACGT	8 cut(s)	1003, 1112, 1310, 2440, 4138, 5256, 5335, 5408
TaqI	TCGA	9 cut(s)	47, 156, 307, 638, 2143, 2209, 2301, 2366, 5329
TaqII	GACCGA	2 cut(s)	432, 2900
TatI	WGTACW	5 cut(s)	2187, 2855, 4195, 4318, 5188
TauI	GCSGC	6 cut(s)	28, 340, 677, 2276, 2821, 3901
TseFI	GTSAC	7 cut(s)	1303, 1937, 2425, 3084, 3485, 3860, 3933
Tsp45I	GTSAC	7 cut(s)	1303, 1937, 2425, 3084, 3485, 3860, 3933
TspGWI	ACGGA	4 cut(s)	76, 112, 3088, 5197
VpaK11BI	GGWCC	8 cut(s)	386, 444, 489, 1676, 2042, 2912, 3157, 4832
VspI	ATTAAT	1 cut(s)	1472
XagI	CCTNNNNNAGG	1 cut(s)	5057
XbaI	TCTAGA	2 cut(s)	3281, 3746
XceI	RCATGY	6 cut(s)	1696, 1824, 2609, 4363, 5033, 5342
XcmI	CCANNNNNNNNNTGG	1 cut(s)	968
XmaJI	CCTAGG	1 cut(s)	1090
XmiI	GTMKAC	1 cut(s)	4132
XmnI	GAANNNNTTC	2 cut(s)	458, 3193
ZraI	GACGTC	1 cut(s)	1308
ZrmI	AGTACT	2 cut(s)	2857, 4197
Zsp2I	ATGCAT	3 cut(s)	6, 19, 994

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.